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n ou ng o en a oSome Marine Organisms
fromIndia Against Species ofBacillus and Pseudomonas
S.H. Bhosale, V.L. Nagle, and .!. "agtapMarine Biotechnology 4, 111118, 2002 Springer-Verlag,
New York Inc
Anggun Ai#la No$a %&'()')*++-
Hasari Ingesti /idati %&0()0*1()+-
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P2N3AH4L4AN
Se5umlah esar organisme laut menghasil6aneragai ahan 6imia untu6 tu5uanpertahanan 77 Metaolit Se6under
8Fouling organism9 men#ea6an6erusa6an laut secara esar untu66epentingan 6omersial
4ntu6 menghindari pertumuhanmen5atuh6an organisme pada stru6tur laut77 memeri6sa proliferasi pere6at mi6roa,Bacillus dan Pseudomonas
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M2O32
:ollection,Identi;cation, and
Methanol2
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and Methanol2ole6si, identi;6asi dan 26stra6si metanol
organisme laut
iga puluh tu5uh spesies organisme laut %?ora danfauna- dari eragai pemu6iman sepan5angpesisir India, 6edalaman *@0(m dengan scua
di$ing.
Sampel dicuci dengan air ta=ar
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:ollection, Identi;cation, andMethanol2ole6si Identi;6asi26stra6si
dalam metanol1(
Lo6asi & !u5arat %+ Spesies-
Lo6asi 0 Mumai %&spesies-Lo6asi ' Catnagiri %'spesies-Lo6asi + Mal$an %+ spesies-Lo6asi * !oa %D spesies-
Lo6asi ) >ar=ar %' spesies-Lo6asi uticorin %'
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Eloral sampel di6ering6an di a=ah naungan dan die6stra6sidalam 1( larutan metanol.
26stra6si sampel he=an #aitu dipotong atau dihancur6an%potongan@potongan 6ecil- A6hir e6stra6si pada 1( larutanmetanol oleh metode perlo6asi 0 atau ' 6ali pada 8suhuamient9
26stra6 ini telah menguap er6urang di a=ah te6ananmengguna6an 6onsentrasi unit %Aspirator A@'S 2#ela
o6#o Ci6a6i6ai Ltd-
26stra6 metanol disimpan dalam otol 6aca disteril6an dandiguna6an untu6 analisis sifat antia6teri mere6a.
Spesimen tunggal diguna6an untu6 e6stra6si diaadi6an di airlaut dengan + formalin dan disimpan di a
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Antiacterial Studies =ithBioe
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hat!an "#$%&' (lter paper )i*c* o+ -!! )ia!eter
Stock Soltion &akra! )ite!patkan pa)a .gar /oell )engan p ,3
/ona ihii*i )ikr )ia!eter"!!' pa)a ek*trak 00 5g per )i*k
Stan)ar) )i*c* o+ penicillin-# "1 6 per )i*c', penicillin-#
"10 6 per )i*c', *trepto!ycin "10 5g per )i*c' an) a!picillin
"10 5g per )i*c' were
hii i f h
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Inhiition of Attachmentof Bacteria on !lass
Slides # Acti$e 2ultur murni di6ultur dalam &(( ml lau dalam Foells.
Setelah 0+ 5am in6uasi uda#a ini %stoc6- diguna6anuntu6 percoaan.
Sepersepuluh dari 6ultur diino6ulasi dalam &(( ml lauerentu6 6erucut #ang mengandung )( ml 6aldu Foells.3ua slide 6aca memili6i luas .0 cm0 %) cm G &.0 cm-masing@masing ditempat6an dalam termos.
Bioe
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Setelah 0+ 5am in6uasi, slide
6aca tunggal dihapus dari lausetiap e6sperimental dan
dicuci dengan 5umlah minimumsteril laut untu6 menghapusmedia dan longgar terpasang
a6teri.
Setelah +D 5am in6uasi untu6slide 6aca #ang tersisa.
Ba6teri dihitung oleh serialpengenceran seperti #ang
di5elas6an seelumn#a %:ollinsdan a#lor, &1)-.
sepersepuluh & G &(@+6onsentrasi %(.&@ml- dari serial
pengenceran di atas, adalaherlapis di piring petri steril
%Foells laut agar@agar-mengguna6an pen#ear.
"umlah a6teri 6oloni %cfu-dihitung setelah 0+ 5am dan
din#ata6an seagai 5umlah cfuper sentimeter persegi.
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HASIL
Padinatetrastromatica
Stoechospermummarginatum
Alga Colat Alga !erah
Amphiroa"ragilissima
Cheliosporumsp.
Chondria
armata #aniaadherence
&) Spesies Elora
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HASIL
00 Spesies Eauna
$orgonian
%chinogorgiapseudoramopa $orgonian sp.
!olusa
Aplysia sp. Plane&is sulcatus
Aulenella"oramini"era 'aliclona sp. Petrosia sp. Psammaplysilla
purpurea Tandinia sp. Spongia o(cinalis
SpongesSo"t Corals
)o*ophytum sp.
Sarcophyton sp. Sinularia sp. Sinularia compressa Sinularia gi**erosa Sinularia ma&ima
Lainn#a
Bryo+oaAscidian sp.,adianthus sp.PentacerasterappinisCassiopeia sp.
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Strain a6teri #angdiguna6an
Bacilluscereus
Bacilluscirculans
Bacilluspumilus
Pseudomonasvesicularis
Pseudomonas putida
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Mildl# acti$e, @Dmm clear Kone
%- moderatel# acti$e, D@1mm clear Kone
%- signi;cantl# acti$e, 1@&&mm clear Kone
%- strongl# acti$e, &&@&'mm clear Kone
Hasil Pengu5ianterhadap
' 5enis sampel
H il P 5i
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Mildl# acti$e, @Dmm clear Kone
%- moderatel# acti$e, D@1mm clear Kone
%- signi;cantl# acti$e, 1@&&mm clear Kone
%- strongl# acti$e, &&@&'mm clear Kone
Hasil Pengu5ianterhadap
' 5enis sampel
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HASIL
26stra6 Psammaplysillapurpurea ersifat strongl#acti$e terhadap a6teriBacillus circulans
26stra6 Cassiopeia sp.ersifat signi;cantl# acti$e terhadapa6teri Pseudomonas putida
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HASILa.A6ti$itas antifouling
Psammaplysillapurpurea danPetrosia sp.
terhadap 5umlah6oloni Bacilluscirculans
*.Ativitas anti"ouling
Psammaplysillapurpurea danPetrosia sp.terhadap 5umlah
6oloni Pseudomonasputida
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Antiioti6 #ang diguna6an
Penicillin@!
& 4disc
Penicillin@!
&( 4disc
Streptom#cin
&( gdisc
Ampicillin
&( gdisc
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antia6teri
Bacilluscereus
Streptom#cin&(
gdisc
Bacilluscirculans
Streptom#cin &(
gdisc
Ampicillin&
( gdisc
Penicillin@
!&( 4disc
Penicillin@
! & 4disc
Bacilluspumilus
Streptom#cin &(
gdisc
Ampicillin&( gdisc
Pseudomonas
vesicularis
Streptom#cin &(
gdisc
Pseudomon
as putida
Streptom#cin &(
gdisc
Ampicillin&
( gdisc
Penicillin@
!&( 4disc
Penicillin@
! & 4disc
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Cymodocea rotundata
Stoechospermum
marginatum
Psammaplysilla
purpurea
Sinularia compressa
Petrosia sp.
Cassiopeia
sp.
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& 3 P il i 6 t
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&. 3ea Pengamilan spesies aca6 atauditarget6an
3ila6u6an secara aca6
0. #len Spesies #ang lain apa tida6memili6i 6emampuan antifouling
Spesies #ang lain 5uga memili6i 6emampuan
antifouling, namun han#a ) spesies terseut #angmemili6i 6emampuan menghamat pertumuhan6elima strain a6teri #ang diguna6an
'. Sarah >enapa diguna6an strain a6teri
terseut >elima strain a6teri terseut menghasil6an io;lm
#ang dapat men5adi sustrat untu6 mele6atn#a"ouling organisms
Q A