37
DAFTAR PUSTAKA
Akuodor, G.C., Idris-Usman M., Ugwu, T.C., Akpan, J.L., Irogbeyi L.A.,Iwuanyanwu, T.C., and Osunkwo U.A., 2010, Ethanolic leaf extract ofVerbena hastata produces antidiarrhoeal and gastrointestinal motilityslowing effects in albino rats,Journal of Medicinal Plants Research,4, 16,1624-1627.
Anarsis, W.,1999, Agribisnis Komoditas Salak, 2, Bumi Askara, Jakarta,
Andersen, Q.M., and Markham, K.R., 2006, Flavonoids, Chemistry, Biochemistryand Applications, 2, CRC Press, USA.
Anderson, J. E., Goetz, C. M., McLaughlin, J. L., and M. A. Suffness, 1991, “Ablind comparison of simple bench-top bioassays and human tumour cellcytotoxicities as antitumor prescreens,” PhytochemicalAnalysis, vol. 2, no.3, pp. 107–111
Anief, M., 2006, Ilmu Meracik Obat, Gadjah Mada University Press, Yogyakarta,169.
Aralas, S., Mohamed, M., and Bakar, M. F. A., 2009, Antioksidant Properties ofSelected Salak (Salacca Zalacca) Varieties in Sabah Malaysia, Nutritionand Food Science, 39
Atta., AH. Mouneir SM., 2004, Antidiarrhoeal activity of some Egyptianmedicinal plant extracts. Journal of Ethnopharmacology; 92; 303-309.
Backer, C.A., and Van de Brink, R.C.B., 1965, Flora of java,Volume II,Published Under the Auspeces of the Rijkher Barium, Leiden, 250-252.
Balaji, G., Chalamaiah, M., Reddy, Y.A., 2012, Antidiarrhoeal Activity ofEthanol and Aqueous Extracts of Carum copticum Seeds in ExperimentalRats, Asian Pacific Journal of Tropical Biomedicine, 1-5.
Carlo, G. D., Autore, G., Izzo, A. A, Maiolino, P., Mascolo, N., Viola P., Diurno,M. V., and Capasso, F., 1993, Inhibition of Intestinal Motility and secretionby Flavonoids in Mice and Rats; Structure-Activity Relationship, Journalof Pharmacy and Pharmacology, 45 (12), 1054-1059
Chitme, H.R., Chandra, R., Kaushik, S., 2004, Studies on anti-diarrhoeal activityof calotropis gigantea r.br.in experimental animals.7(1):70-75
Cseke, L.J., Kirakosyan, A., Kaufman, P.B., Warber, S.L., Duke, J.A., Brielmann,H.L., 2006, Natural Products from Plants, Second Edition, America, 25-27.
38
Depkes RI, 1985,Cara Pembuatan Simplisia, Departemen Kesehatan RepublikIndonesia, Jakarta, 3.
Depkes RI, 1986, Sediaan Galenik, Departemen Kesehatan Republik Indonesia,Jakarta, 6-7
Depkes RI, 2000, Parameter Standar Umum Ekstrak Tumbuhan Obat, CetakanPertama, 5, Departemen Kesehatan Republik Indonesia, Jakarta.
Depkes RI,2011,Buku Saku Petugas Kesehatan, 2, Departemen KesehatanRepublik Indonesia, Jakarta.
Dipiro, J.T., Talbert, R.L., Yee, G.C., Matzke, G.R., Wells, B.G., and Posey, L.M.,2005, Pharmacoterapy A Phatophysiology Approach, sixth edition, 677,Mc Graw Hill, USA.
Farthing, M.J.G., 2006, Antisekretory Drugs for Diarrheal Disease, ReviewArticle, Dig Dis, 24, 47-58
Federer, W., 1991, Statistics and Society: Data Collection and Interpretation,Second Edition, Marcel Dekker, New York.
Foegh, M.L., and Ramwell, P.W., 2001, The Eicosanoids: Prostaglandins,Thromboxanes, Leucotrienes, and Plateled Coumpounds dalam Katzung,B.G., Basic and Clinical Pharmacology, McGraw-Hill, USA, 316.
Hamilton, J.W., 2012, Pengaruh Pemberian Ekstrak Dimer Isoeugenol secara Oralterhadap Kualitas Spermatozoa Mencit (Mus musculus L.) Jantan GalurDDY, Skripsi, Universitas Indonesia, Depok.
Harborne, J.B., 1987., Metode Fitokimia, Penerbit ITB, Bandung, 102-105.
Havagiray, R., Ramesh, C., and Sadhna, K., 2004, Study of antidiarrhoeal activityof Calotropis gigantea R.B.R. in experimental animals, Journal ofPharmacy Pharmaceutical Science; 7: 70-75.
Heinrich, M., Barnes, J., Gibbons, S., Williamson, E. M., 2010, Farmakognosidan Fitoterapi, EGC, Jakarta, 6.
Hoegger, R., 1998,Training Papers Distillation with a Rotary Evaporator, 3-4,BUCHI Labortechnik AG, Switzerland.
Hossain, H., Hassan, M., Jahan, I.A., Nimmi, I., and Islam, A.,2012,Antidiarrhoeal Activity,Nitric Oxide Scavenging and Total TanninContent from the Bark of Ceriops decandra (Griff.) Ding Hou,InternationalJournalof Pharmaceutical Science and Research,1306-1311.
39
Ikawati, Z., 2008, Pengantar Farmakologi Molekuler, Gadjah Mada University Press,Yogyakarta, 50, 78-81.
Joseph, B, and Priya, M., 2011, Review on Nutritional and PharmacologicalProperties of Guava (Psidium guava Linn.), International Journal ofPharma and Bio Sciences, 1-7.
Kalaskar, M.G., Divekar, V.B., Chaugule, P.D., Surana, S.J., and Baheti, D.G., 2010,Studies On Anti-diarrheal Activity of Dalberjia sissooRoxb. inExperimental Animals, Pharmacologyonline, 1, 453-457.
Katzung, B.G., 2004, Farmakologi Dasar dan Klinik, diterjemahkan oleh StafDosen Farmakologi dan Fakultas Kedokteran Universitas Airlangga, EdisiVIII, 552-553, Salemba Medika, Jakarta.
Khairunnisa, 2011, Perbedaan Aktivitas Senyawa Antibakteri Ekstrak Teh Hijaudan Teh Hitam Terhadap Staphylococcus aureus Secara In Vitro,Skripsi,Universitas Pembangunan Nasional “Veteran’’, Jakarta.
Kumar, R., 1983, Chemical and Biochemical Nature of Fodder Tree Tannins.Journal of Agricultural and food chemistry, 31, 1364-1366.
Leontowich, M., Leontowich, H., Drzewiecki, Z., Haruenkit, R., Poovarodom, S.,Park, Y. S., Jung, S. T., Kang, S. G., Trakhtenberg, S., and Gorinstein, S.,2007, Two Exotic Fruits Positively Affect Rat’s Plasma Composition, FoodChemistry, 192-200.
Longanga, O.A., Vercruysse, A., and Foriers, A., 2000, Contribution to TheEthnobotanical, Phytochemical and Pharmacological Studies ofTraditionally Used Medicinal Plants in The Treatment of Dysentery andDiarrhoea in Lomela Area, Democratic Republic of Congo (DRC), JournalEthnopharmacol, 71, 3, 411-23.
Lutterodt, G.D., 1989, Inhibition of Gastrointestinal Release of Acetylcholine byQuercetin as a Possible Mode of Action of Psidium guajara leaf Extracts inthe Treatment of Acute Diarrhoea Disease, Journal Ethnopharmacology, 23,235-247.
Mandrowatie, E., 2016, Uji Efek Antimotilitas Usus Ekstrak Etanol Buah SalakPondoh (Salacca zalacca (Gaertn.)Voss) Pada Mencit Jantan Galur Balb-C,Skripsi, Universitas Wahid Hasyim, Semarang.
Mutshler, E., 1991, Dinamika Obat, ITB Bandung, 521-549.
40
Ningsih, K.A., 2013, Uji Efek Antisekretori Ekstrak Etanol Daun Randu (Ceibapentandra (L.)Gaertn.) Pada Mencit Jantan Galur Balb/C Dengan MetodeCastor Oil Induced Enteropooling, Skripsi, Universitas Wahid Hasyim,Semarang
Nurina, C.I.A., Samingan., Iswandi., 2014, Uji Anti Mikroba Ekstrak Buah Salak(salacca edulis) Terhadap Bakteri Escherichia coli, 19-23.
Organization, W.H., 2004, World Health Report, WHO, 120-125.
Palombo, E.A., 2006, Phytochemicals from Traditional Medicinal Plants Used inthe Treatment of Diarrhoea : Modes of Action and Effects on IntestinalFunction, Phytother Res, 20, 9, 717-724.
Purnawati, D.M., 2006,Pengaruh Pemberian Jus Buah Tomat (licopersicumesculentum mill ) terhadap Jumlah Spermatozoa Mencit Strain Balb/CJantan yang dipapar Asap Rokok, Artikel Ilmiah, Universitas Diponegoro,Semarang.
Purnomo, H., 2000, Budidaya Salak Pondoh, Semarang, 1-5.
Rahman, K., Chowdhury, A U., Islam, M. T., Chrowdhury, A., Uddin, M. E., andSumi, C. D., 2015, Evaluation of antidiarrheal Activity of MethanolicExtract of Marantana aruninacea Linn. Leaves, Advances inPharmacological Sciences, 1-7
Robert, A., Nezamis, J.E, Lancaster, C., Hanchar, A.J., Klepper, M.S., 1976,Enteropooling assay; A test for diarrhea procedured by prostaglandin,Elsevier,11, 809-828.
Sahputra, F.M., 2008, Potensi Ekstrak Kulit dan Daging Buah Salak SebagaiAntidiabetes, Skripsi, Fakultas Matematika dan Ilmu Pengetahuan AlamIPB, Bogor.
Sanchez, M.F., Galvez, J., Gonzalez, M., Zarzuelo, A., and Barrett, K.E., 1997,Effects of quercetin on epithelial chloride secretion, Life Science, 61:2049-2055
Sarker, S.D., Nahar, L., 2009, Kimia untuk Mahasiswa Farmasi, diterjemahkanoleh Abdul Rohman, Pustaka Pelajar, Yogyakarta, 528.
Saunders, 2007, Antisekretori, Dorland’s Medical Dictionary for HealthConsumers, diambil dari http://www.thefreedictionary.com/sources.htm,diakses tanggal 20 oktober 2016.
41
Schott, C., MD, and Bono, M.J., MD, FACEP.,2011, Evaluation and Managementof Diarrhea in the ED,7-8.
Shiramane, R.S., Biradar, K.V., Chivde, B.V., Shambhulingayya, H.M., andGoud, V., 2011, In-vivo Antidiarrhoeal Activity of Ethanolic ExtractofDelonix Regia Flowers in Experimental Induced Diarrhoea inWistarAlbino Rats, International Journal of Research in Pharmacy AndChemistry, 1, 3, 2231-2781.
Shoba., FG., Thomas M., 2001, Study of antidiarrhoeal activity of four medicinalplants in castor oil induced diarrhoea, Journal of Ethnopharmacology, 76:73-76.
Sibuea, F.S.Y., 2015, Ekstraksi Tanin Dari Kluwak (pangium edule R.)Menggunakan Pelarut Etanol dan Aquades dan Aplikasinya sebagaiPewarna Makanan,Skripsi,Universitas Negeri Semarang, Semarang
Suhendi, A., Nurcahyanti, Muhtadi, dan Sutrisna, E.M., 2011, AktivitasAntihiperurisemia Ekstrak Air Jinten Hitam (Coleus ambonicus Lour) padaMencit Jantan Galur Balb-C dan Standardisasinya,Majalah FarmasiIndonesia, 22, 2, 77 – 84.
Suskendriyati, H., Wijayanti, A.,Hidayah, N.,Cahyuningdari,D.,2000,StudiMorfologi dan Hubungan Kekerabatan Varietas Salak Pondoh (Salaccazalacca (Gaert.) Voss) di Dataran Tinggi Sleman, 59-64.
Sutadi, S.M., 2003, Diare Kronik, Universitas Sumatera Utara, 2.
Syder, JD., and Merson, M.H, 1982, The magnitude of the global problem ofacute diarrheal disease; a riview of active surveillance data Bulletin of theWorl Health Organization; 60: 605-13.
Teja, J.N., Pradeep, D., Sumanth, N., Kumar, G.V., 2012, Anti-diarrhoeal Activityof Petroleum Ether extract of vanda Tessellata Leaves on castor oil-inducedDiarrhea in rats, 3, 99-102.
Tjay, T.H., dan Rahardja, K., 2007, Obat-Obat Penting; Khasiat, Penggunan, danEfek-efek Sampingnya, Edisi 4, Elex Media Komputindo, Jakarta, 288-297.
Tripathi, K.D., 1994, Essentials of Medical Pharmacology, Jaypee BrothersMedical Publishers (P), New Delhi, 775.
Tunarua, S., Althoff, T.F., Nusing, R.M., Diener, M., dan Offermannsa., S., 2012,Castor oil induces laxation and uterus contraction via ricinoleic acidactivating prostaglandin EP3 receptors, Germany, 9179–9184.
42
Veiga, Y. F., Zunino, L., Calixto, J. B., Pititucci, M. L., Pinato, A. C.,Phytochemical and antioedematogenic studies of commercial copaiba oilsavaible in Brazil. Phytother. Res. 15 (6), 476- 480
Voigt, R., 1995, Buku Pelajaran Teknologi Farmasi, Edisi 5, Gadjah MadaUniversity Press, Yogyakarta,551-584.
Wibowo, A., 2011,Efek Ekstrak Etanol Daging Buah Salak Pondoh(Salaccaedulis Reinw.)Sebagai Antidiare Pada Mencit Swiss Webster Jantan YangDiinduksi OleumRicini, Skripsi,Universitas Kristen Maranatha.
Wirakusumah, E. S., 2006, Buah dan Sayur untuk Terapi, Jakarta, 61-62.
Woodley, M.D., and Alison, M.D., 1995, Pedoman Pengobatan, YayasanEssentia Medica, Yogyakarta, 429-446.
Yasmeen, M., Prabhu B., Agashikar, N.V., 2010, Evaluation Of TheAntidiarrhoeal Activity Of The Leaves Of IxoraCoccinea Linn. In Rats,Journal Of Clinical and Diagnostic Research, 4, 3298-3303.
43
LAMPIRAN
43
Lampiran 1. Surat Keterangan Hasil Determinasi Tanaman Salak
44
Lampiran 1. Lanjutan
45
Lampiran 1. Lanjutan
46
Lampiran 2. Surat Keterangan Telah Melakukan Pemekatan Ekstrakdengan Rotary Evaporator di Laboratorium Fitokimia FakultasFarmasi Universitas Wahid Hasym Semarang
47
Lampiran 3. Surat Keterangan Telah Selesai Melakukan Penelitian diLaboratorium Farmakologi dan Toksikologi Fakultas FarmasiUniversitas Wahid Hasyim Semarang
48
Lampiran 4. Data Penimbangan Bahan
1. CMC-Na 0,5%
Berat kertas saring + CMC-Na = 0,845 mg
Berat kertas saring = 0,335 mg
Berat CMC-Na = 500mg/100mL
2. Ekstrak Etanol Buah Salak
Berat cawan porselen + ekstrak kental buah salak =32,888 gram
Berat cawan porselen =32,706 gram
Berat ekstrak kental buah salak = 0,182gram
3. Ekstrak Etanol Buah Salak
Berat cawan porselen + ekstrak kental buah salak =33,070 gram
Berat cawan porselen =32,706 gram
Berat ekstrak kental buah salak = 0,364 gram
4. Ekstrak Etanol Buah Salak
Berat cawan porselen + ekstrak kental buah salak =33,434 gram
Berat cawan porselen =32,706 gram
Berat ekstrak kental buah salak = 0,728 gram
49
Lampiran 5. Perhitungan Dosis Larutan Uji dan Volume Pemberian
a. Larutan kontrol negatif tanpa perlakuan (larutan NaCl 0,9%)
Dosis (mg) untuk mencit seberat 20g = mLmLxg
g5,025
1000
20
Jadi volume penyuntikan larutan NaCl sebanyak 0,5mL dan diberikan
secara peroral pada mencit seberat 20g.
1. Mencit I
mLmLxg
g517,025
1000
7,20
2. Mencit II
mLmLxg
g502,025
1000
1,20
3. Mencit III
mLmLxg
g562,025
1000
5,22
4. Mencit IV
mLmLxg
g562,025
1000
5,22
5. Mencit V
mLmLxg
g625,025
1000
0,25
50
Lampiran 5. Lanjutan.
b.Kelompok kontrol negatif dengan perlakuan
Kelompok kontrol negatif dengan perlakuan diberikan castor oil dengan
volume penyuntikan sebanyak 1,0 mL peroral.
c. Suspensi ekstrak etanol dosis 182, 364, dan 728 mg/kgBB
Ekstrak etanol buah salak disuspensikan dalam larutan CMC-Na 0,5%.
Larutan CMC-Na 0,5% dibuat dengan cara menimbang 500 mg CMC-Na lalu
dilarutkan dalam aquadest ad 100 ml.
Suspensi ekstrak etanol buah salak dosis 182 mg/kgBB.
Diasumsikan berat mencit 20 g.
1000g
20gmLmgmLmgkgBBmgx /28,75,0/64,3/182
7,28 mg/mL × 25 mL = 182 mg
Perhitungan volume penyuntikan:
1. Mencit I
mgmgxg
g186,4182
1000
0,23
Vp= mLmLmg
mg575,0
/28,7
186,4
2. Mencit II
mgmgxg
g785,3182
1000
8,20
51
Lampiran 5. Lanjutan.
Vp= mLmLmg
mg519,0
/28,7
785,3
3. Mencit III
mgmgxg
g440,4182
1000
4,24
Vp= mLmLmg
mg609,0
/28,7
440,4
4. Mencit IV
mgmgxg
g513,4182
1000
8,224
Vp= mLmLmg
mg619,0
/28,7
513,4
5. Mencit V
mgmgxg
g949,3182
1000
7,21
Vp= mLmLmg
mg542,0
/28,7
949,3
Jadi volume penyuntikan untuk mencit I, II, III, IV, V adalah sebanyak
0,575; 0,519; 0,609; 0,619; 0,542 mL diberikan secara peroral.
52
Lampiran 5. Lanjutan.
Suspensi ekstrak etanol buah salak dosis 364 mg/kgBB.
Diasumsikan berat mencit 20 g.
1000g
20gmLmgmLmgkgBBmgx /56,145,0/28,7/364
14,56 mg/mL × 25 mL = 364 mg
Perhitungan volume penyuntikan
1. Mencit I
mgmgxg
g481,8364
1000
3,23
Vp= mLmLmg
mg582,0
/56,14
481,8
2. Mencit II
mgmgxg
g28,7364
1000
0,20
Vp= mLmLmg
mg5,0
/56,14
28,7
3. Mencit III
mgmgxg
g462,7364
1000
5,20
Vp= mLmLmg
mg512,0
/56,14
462,7
53
Lampiran 5. Lanjutan.
4. Mencit IV
mgmgxg
g881,8364
1000
4,24
Vp= mLmLmg
mg609,0
/56,14
881,8
5. Mencit V
mgmgxg
g28,7364
1000
0,20
Vp= mLmLmg
mg5,0
/56,14
28,7
Jadi volume penyuntikan untuk mencit I, II, III, IV, V adalah sebanyak
0,582; 0,5; 0,512; 0,609; 0,5 mL diberikan secara peroral.
Suspensi ekstrak etanol buah salak dosis 728 mg/kgBB.
Diasumsikan berat mencit 20 g.
1000g
20gmLmgmLmgkgBBmgx /12,295,0/56,14/728
29,12 mg/mL × 25 mL = 728 mg
54
Lampiran 5. Lanjutan.
Perhitungan volume penyuntikan
1. Mencit I
mgmgxg
g579,15728
1000
4,21
Vp= mLmLmg
mg534,0
/12,29
579,15
2. Mencit II
mgmgxg
g161,16728
1000
2,22
Vp= mLmLmg
mg554,0
/12,29
161,16
3. Mencit III
mgmgxg
g326,17728
1000
8,23
Vp= mLmLmg
mg594,0
/12,29
326,17
4. Mencit IV
mgmgxg
g399,17728
1000
9,23
Vp= mLmLmg
mg597,0
/12,29
399,17
55
Lampiran 5. Lanjutan.
5. Mencit V
mgmgxg
g215,15728
1000
9,20
Vp= mLmLmg
mg522,0
/12,29
215,15
Jadi volume penyuntikan untuk mencit I, II, III, IV, V adalah sebanyak
0,534; 0,554; 0,594; 0,597; 0,522 mL diberikan secara peroral.
d. Perhitungan Persentase Efek Antisekretori
1. Dosis 182mg
a. Mencit I
%05,41%10095,0
56,095,0
x
b. Mencit II
%89,37%10095,0
59,095,0
x
c. Mencit III
%84,36%10095,0
60,095,0
x
d. Mencit IV
%84,36%10095,0
60,095,0
x
56
e. Mencit V
%74,34%10095,0
62,095,0
x
2. Dosis 364 mg
a. Mencit I
%47,49%10095,0
48,095,0
x
b. Mencit II
%84,36%10095,0
60,095,0
x
c. Mencit III
%74,34%10095,0
62,095,0
x
d. Mencit IV
%74,34%10095,0
62,095,0
x
e. Mencit V
%63,32%10095,0
64,095,0
x
3. Dosis 728 mg
a. Mencit I
%10,42%10095,0
57,095,0
x
57
Lampiran 5. Lanjutan.
b. Mencit II
%58,31%10095,0
65,095,0
x
c. Mencit III
%53,30%10095,0
66,095,0
x
d. Mencit IV
%53,30%10095,0
66,095,0
x
e. Mencit V
%47,29%10095,0
67,095,0
x
58
Lampiran6. Data Volume Isi Usus
mencit Volume isi usus (mL)KNTP KNDP EEBS 1 EEBS 2 EEBS 3
1 0,10 0,90 0,56 0,48 0,572 0,14 0,91 0,59 0,60 0,65
3 0,15 0,92 0,60 0,62 0,66
4 0,15 1,00 0,60 0,62 0,66
5 0,21 1,03 0,62 0,64 0,67
rata-rata± SE 0,15±0,018 0,95±0,026 0,59±0,010 0,59±0,029 0,64±0,018
Keterangan :Kntp : NaCl 0,9% 25 mL/kgBBKndp : castor oil 1,0 mL.EEBS 1 : ekstrak etanol buah salak pondoh dosis 182 mg/kgBB + castor oil 1,0 mL.EEBS 2 : ekstrak etanol buah salak pondoh dosis 364 mg/kgBB + castor oil 1,0 mL.EEBS 3 : ekstrak etanol buah salak pondoh dosis 728 mg/kgBB +castor oil 1,0 mL.
59
Lampiran 7. Data Persentase Efek Antisekretori
mencit %Efek antisekretoriEEBS 1 EEBS 2 EEBS 3
1 41,05 49,47 42,102 37,89 36,84 31,583 36,84 34,74 30,53
4 36,84 34,74 30,53
5 34,74 32,63 29,47rata-rata± SE 37,47 ± 1,030 37,64 ± 3,020 32,84 ± 2,338
Keterangan :EEBS 1 : ekstrak etanol buah salak pondoh dosis 182 mg/kgBB + castor oil 1,0 mL.EEBS 2 : ekstrak etanol buah salak pondoh dosis 364 mg/kgBB + castor oil 1,0 mL.EEBS 3 : ekstrak etanol buah salak pondoh dosis 728 mg/kgBB +castor oil 1,0 mL.
60
Lampiran 8. Hasil Uji Normalitas dan Homogenitas Data Volume Isi UsusSecara Statistik
Descriptives
volume usus
N Mean
Std.
Deviation Std. Error
95% Confidence Interval for Mean
Minimum MaximumLower Bound Upper Bound
kntp 5 .1500 .03937 .01761 .1011 .1989 .10 .21
kndp 5 .9520 .05891 .02634 .8789 1.0251 .90 1.03
dosis182 5 .5940 .02191 .00980 .5668 .6212 .56 .62
dosis364 5 .5920 .06419 .02871 .5123 .6717 .48 .64
dosis728 5 .6420 .04087 .01828 .5913 .6927 .57 .67
Total 25 .5860 .26470 .05294 .4767 .6953 .10 1.03
Tests of Normality
Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
kntp .300 5 .161 .920 5 .530
kndp .307 5 .140 .842 5 .171
eedbs182 .228 5 .200* .932 5 .607
eedbs364 .350 5 .045 .750 5 .030
eedbs728 .378 5 .019 .710 5 .012
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Volume isi usus tidak terdistribusi normal pada dosis 364, 728 mg/kgBB.
Test of Homogeneity of Variances
volume usus
Levene Statistic df1 df2 Sig.
1.504 4 20 .239
Volume isi usus memiliki varian yang homogen
61
Lampiran 8. LanjutanKruskal-Wallis Test
Ranks
kelompok N Mean Rank
volume usus kntp 5 3.00
kndp 5 23.00
dosis182 5 10.40
dosis364 5 12.20
dosis728 5 16.40
Total 25
Test Statisticsa,b
volume usus
Chi-Square 20.290
df 4
Asymp. Sig. .000
a. Kruskal Wallis Test
b. Grouping Variable: kelompok
Kelompok kontrol negatif dengan perlakuan menunjukan hasil yang berbedabermakna (p<0,05) dengankelompok yang diberi perlakuan ekstrak etanol buahsalak pondohdan mempunyai nilai signifikasi 0,000 (p<0,05).Dapat ditarik kesimpulan bahwa kelompok perlakuan yang diberi ekstrak etanolbuah salak pondoh memiliki efek antisekretori.
Mann-Whitney Test
Ranks
kelomp
ok N Mean Rank Sum of Ranks
volume usus kntp 5 3.00 15.00
kndp 5 8.00 40.00
Total 10
62
Lampiran 8. Lanjutan
Test Statisticsb
volume usus
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.619
Asymp. Sig. (2-tailed) .009
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: kelompokHasil uji Mann-Whitney menunjukan kelompok kontrol negatif tanpa perlakuanberbeda bermakna (p<0,05) dengan kelompok kontrol negatif dengan perlakuan.Dapat ditarik kesimpulan bahwa kelompok kontrol negatif dengan perlakuanmampu menginduksi sekresi cairan dan elektrolit ke dalam usus.
Ranks
kelompok N Mean Rank Sum of Ranks
volume usus kndp 5 8.00 40.00
dosis182 5 3.00 15.00
Total 10
Test Statisticsb
volume usus
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.619
Asymp. Sig. (2-tailed) .009
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: kelompok
63
Lampiran 8. Lanjutan
Ranks
kelompok N Mean Rank Sum of Ranks
volume usus kndp 5 8.00 40.00
dosis364 5 3.00 15.00
Total 10
Test Statisticsb
volume usus
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.619
Asymp. Sig. (2-tailed) .009
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Ranks
kelompok N Mean Rank Sum of Ranks
volume usus kndp 5 8.00 40.00
dosis728 5 3.00 15.00
Total 10
Test Statisticsb
volume usus
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.619
Asymp. Sig. (2-tailed) .009
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: kelompok
64
Lampiran 8. Lanjutan
Ranks
kelompok N Mean Rank Sum of Ranks
volume usus dosis182 5 4.60 23.00
dosis364 5 6.40 32.00
Total 10
Test Statisticsb
volume usus
Mann-Whitney U 8.000
Wilcoxon W 23.000
Z -.964
Asymp. Sig. (2-tailed) .335
Exact Sig. [2*(1-tailed Sig.)] .421a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Ranks
kelompok N Mean Rank Sum of Ranks
volume usus dosis364 5 3.80 19.00
dosis728 5 7.20 36.00
Total 10
Test Statisticsb
volume usus
Mann-Whitney U 4.000
Wilcoxon W 19.000
Z -1.786
Asymp. Sig. (2-tailed) .074
Exact Sig. [2*(1-tailed Sig.)] .095a
a. Not corrected for ties.
b. Grouping Variable: kelompok
65
Lampiran 8. Lanjutan.
Ranks
kelompok N Mean Rank Sum of Ranks
volume usus dosis182 5 3.80 19.00
dosis728 5 7.20 36.00
Total 10
Test Statisticsb
volume usus
Mann-Whitney U 4.000
Wilcoxon W 19.000
Z -1.786
Asymp. Sig. (2-tailed) .074
Exact Sig. [2*(1-tailed Sig.)] .095a
a. Not corrected for ties.
b. Grouping Variable: kelompok
66
Lampiran 9. Hasil Tes Normalitas dan Homogenitas Data %efek antisekretoriSecara Statistik
Descriptives
efek
N Mean Std. Deviation Std. Error
95% Confidence Interval for Mean
Minimum MaximumLower Bound Upper Bound
dosis182 5 37.4720 2.30432 1.03052 34.6108 40.3332 34.74 41.05
dosis364 5 37.6840 6.75462 3.02076 29.2970 46.0710 32.63 49.47
dosis728 5 32.8420 5.22887 2.33842 26.3495 39.3345 29.47 42.10
Total 15 35.9993 5.26431 1.35924 33.0841 38.9146 29.47 49.47
Tests of Normality
kelompok
Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
efek dosis182 .228 5 .200* .931 5 .605
dosis364 .350 5 .045 .750 5 .030
dosis728 .395 5 .010 .684 5 .006
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
%Efek antisekretori tidak terdistribusi normal pada dosis 364, 728mg/kgBB.
Test of Homogeneity of Variances
efek
Levene Statistic df1 df2 Sig.
1.257 2 12 .319
%efek antisekretori memiliki varian yang homogen
67
Lampiran 10. Hasil uji Kruskal-Wallis dan Mann-Whitney %efek
antisekretori
Kruskal-Wallis Test
Ranks
kelompok N Mean Rank
efek dosis182 5 10.40
dosis364 5 8.80
dosis728 5 4.80
Total 15
Test Statisticsa,b
efek
Chi-Square 4.228
df 2
Asymp. Sig. .121
a. Kruskal Wallis Test
b. Grouping Variable:
kelompok
Mann-Whitney TestRanks
kelompok N Mean Rank Sum of Ranks
efek dosis182 5 6.40 32.00
dosis364 5 4.60 23.00
Total 10
68
Lampiran 10. Lanjutan
Test Statisticsb
Efek
Mann-Whitney U 8.000
Wilcoxon W 23.000
Z -.964
Asymp. Sig. (2-tailed) .335
Exact Sig. [2*(1-tailed Sig.)] .421a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Ranks
kelompok N Mean Rank Sum of Ranks
efek dosis364 5 7.20 36.00
dosis728 5 3.80 19.00
Total 10
Test Statisticsb
Efek
Mann-Whitney U 4.000
Wilcoxon W 19.000
Z -1.786
Asymp. Sig. (2-tailed) .074
Exact Sig. [2*(1-tailed Sig.)] .095a
a. Not corrected for ties.
b. Grouping Variable: kelompok
69
Lampiran 10. Lanjutan
Ranks
kelompok N Mean Rank Sum of Ranks
efek dosis182 5 7.00 35.00
dosis728 5 4.00 20.00
Total 10
Test Statisticsb
Efek
Mann-Whitney U 5.000
Wilcoxon W 20.000
Z -1.576
Asymp. Sig. (2-tailed) .115
Exact Sig. [2*(1-tailed Sig.)] .151a
a. Not corrected for ties.
b. Grouping Variable: kelompok
70
Lampiran 11. Dokumentasi Penelitian
a. Timbangan Listrik (HENHERR)
b.Proses pembuatan serbuk buah salak pondoh dengan blender
71
Lampiran 11. Lanjutan
c. Pengecekan kadar air dengan moisture balance
d. Maserat hasil maserasi simplisia buah salak pondoh
72
Lampiran 11. Lanjutan
e. Proses evaporasi dengan menggunakan alat rotary evaporator
f. Ekstrak etanol buah salak
73
Lampiran 11. Lanjutan
g. Castor oil
h. Pemberian sediaan uji secara peroral
74
Lampiran 11. Lanjutan
i. Pembedahan hewan uji
j. Volume isi usus