![Page 1: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/1.jpg)
ANCA Prepared by Dr. Anees Al-SaadiApril 2008
Clinical immunology principles and practice RICH, 2nd editionMethodological update ANCA.mhtwww.medicinenet.com › emedicine.medscape.com/,www.youtube.com
![Page 2: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/2.jpg)
Methodological update ANCA.mht
History ANCA auto antibodies related to
inflammatory disorders.
van der Woude et al. in 1985 showed ANCA related to Wegener's granulomatosis.
![Page 3: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/3.jpg)
Methodological update ANCA.mht
Antigens Enzymes used in the defense
against bacteria found in WBCs granules.
Most reactivities were found molecular weights ≈29kD & l4OkD.
![Page 4: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/4.jpg)
Methodological update ANCA.mht
Antigens
Two main enzymes in ANCA take part in the killing of bacteria by:
Proteinase 3 (PR3) showing cANCA pattern.
Myeloperoxidase (MPO) showing pANCA pattern.
![Page 5: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/5.jpg)
Methodological update ANCA.mht
Proteinase 3 (PR3)
Molecular weight of 29kD antigen: Which is proteinase . PR3 is a single protein chain that is
glycosylated. Is very similar to other serine proteases
like elastase and cathepsin G.
![Page 6: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/6.jpg)
Methodological update ANCA.mht
Myeloperoxidase (MPO)
Myeloperoxidase (MPO)
MPO is a dimer molecular weight of l4OkD consisting of one heavy & light chain .
It is glycosylated with mannose oligosaccharide chains.
Is cleaved by heating and has a green color .
![Page 7: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/7.jpg)
Methodological update ANCA.mht
Myeloperoxidase (MPO)
Many other antigens have been described to be associated with ANCA, i.e. elastase, lactoferrin cathepsin G, BPI defensins, HMG1/2, catalase etc.
![Page 8: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/8.jpg)
Methodological update ANCA.mht
Antigens associated with ANCA
![Page 9: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/9.jpg)
Methodological update ANCA.mht
Development of ANCA
It is poorly understood how ANCA are developed.
Two possible mechanisms of ANCA development are postulated:
Theory of molecular mimicry. Theory of defective apoptosis.
![Page 10: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/10.jpg)
Methodological update ANCA.mht
Development of ANCA
Theory of molecular mimicry.
Superantigens have the power to stimulate a strong immune response . THEY have regions that resemble self-antigens – this is the theory of molecular mimicry.
classical example in post group A streptococcal rheumatic heart disease, where there is similarity between M proteins of Streptococcus pyogenes to cardiac myosin and laminin.
Theory of defective apoptosis.
ANCA may be developed either via ineffective apoptosis or ineffective removal of apoptotic cell fragments, leading to the exposure of the immune system to molecules normally sequestered inside the cells. This theory solves the paradox of how it could be possible for antibodies to be raised against the intracellular antigenic targets of ANCA.[4]
![Page 11: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/11.jpg)
Methodological update ANCA.mht
Three patterns of ANCA
p-ANCA, show a perinuclear staining pattern
c-ANCAs, show a diffusely granular, cytoplasmic staining pattern
Atypical that develop against antigens other than MPO or PR3 will occasionally result in patchy staining
![Page 12: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/12.jpg)
Methodological update ANCA.mht
pANCA disease association :
• Primary vasculitisMicroscopic polyangitis Churg-Strauss syndrome Polyarteritis nodosa
• CollagenosisFelty's syndromeSLERheumatiod arthritisSjögren's syndrome
• Chronic inflammatory bowel disease
• Chronic liver disease
Primary sclerosing cholangitis
cANCA disease : association
• Primary vasculitis
• Wegener's granulomatosis
• Microscopic polyangitis
• Churg-Strauss syndrome.
Atypical
• Primary sclerosing cholangitis
• Primary biliary cirrhosis
• Autoimmune hepatitis
• SLE
• RA
![Page 13: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/13.jpg)
Wegener's granulomatosis
Definition
ClinicalPicture
Treatment
Wegener's granulomatosis is a uncommon type of (vasculitis). It classically involves the lungs, the nasal passages (sinuses), and the kidneys.
Nasal or oral inflammation: Lungs: abnormal chest X-ray .Kidneys: with microhematuria or red cell casts Biopsy: granulomatous inflammation Initial treatment is corticosteroids and oral CYC.On remission treatment is changed to azathioprine or methotrexate.Duration of therapy should be at least one year.
![Page 14: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/14.jpg)
Methodological update ANCA.mht
Microscopic polyangitis
Definition • Microscopic
polyangiitis (MPA) is vasculitis of small vessels.
Features• Renal GN
79%.• May progress
to irreversible rapid progressive renal damage If not treated .
• Weight loss 73%.
• Fever 55%.
• Pulmonary: alveolar hemorrha
ge.
Treatment• daily/
intermittent I.v cyclophosphamide 6-12months .
• Methotraxate or azathioprine in remission stage of the disease.
![Page 15: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/15.jpg)
Methodological update ANCA.mht
Churg-Strauss syndrome .
Definition
• An eosinophil-rich form of granulomatous inflammation mainly involving respiratory tract
• Asthma and eosinophilia.
Features
• 1. A prodromal phase• Allergic
disease.
• 2. Eosinophilia-tissue infiltration phase:• Eosinophilia.
• 3. Vasculitic phase : • Systemic
necrotizing vasculitis .
Treatment
• Treatment is prednisone for mild cases , systemic involvement cyclophosphamide to be added.
• Clinical remission seen in 90% of the cases and recurrences seen in 25%
![Page 16: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/16.jpg)
Applications of Antineutrophil Cytoplasmic Antibody.mht
Applications of ANCA
The application of ANCA useful in both the diagnosis and monitoring of disease activity .
![Page 17: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/17.jpg)
Methodological update ANCA.mht
Applications of ANCA Churg-Strauss Syndrome
Wegener's Granulomatosis
Microscopic Polyangiitis ANCA
10% 80% 40 % PR3-ANCA
60% 20% 50% MPO-ANCA
30% 5% 10% Negative
![Page 18: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/18.jpg)
Applications of ANCA Prevalence of ANCA in renal
vasculitis ANCA test positivity (%) Type of renal
vasculitis C-ANCA P-ANCA
10-20% 10-20% Polyarteritis nodosa
10-20% 50-80% Microscopic polyangitis
80-90% 10-20% Wegener`s granulomatosis
10-20% 50-80% Necrotizing and crescentic GN
Clinical immunology principles and practice RICH, 2nd edition .
![Page 19: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/19.jpg)
Applications of ANCA
In Juvenile rheumatoid arthritis : Occur in 10-20% of active conditions. Showing atypical pattern. Does not correlate with clinical
parameters.
Clinical immunology principles and practice RICH, 2nd edition pr
![Page 20: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/20.jpg)
Applications of ANCA
Autoimmune hepatitis : Showing p-ANCA pattern. Seen in 65%-96% in AIH-1 not in AIH-
2. Not correlated with disease activity and
liver function test.
Clinical immunology principles and practice RICH, 2nd edition pr
![Page 21: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/21.jpg)
Applications of ANCA
Inflammatory bowel disease: Showing p-ANCA pattern more seen
in UC than CD. Not been used for distinguishing the 2
diseases .
Clinical immunology principles and practice RICH, 2nd edition pr
![Page 22: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/22.jpg)
AccessMedicine - ANCA induced by drugs.mht
Applications of ANCA Drug-induced ANCA:
Picture of ANCA-associated vasculitis (AAV).
Propylthiouracil is a well-documented cause of drug-induced AAV.
Other drugs:▪ Hydralazine,sulfasalazine.▪ minocycline, D-penicillamine.▪ ciprofloxacin, phenytoin.▪ clozapine, allopurinol, and pantoprazole.
![Page 23: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/23.jpg)
AccessMedicine - ANCA induced by drugs.mht
Applications of ANCA
Usually of p-ANCA pattern.
Slow resolving condition.
Good responding to low dose of immunosuppressive agents.
![Page 24: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/24.jpg)
Applications of ANCA
Prognostic significance of ANCAs:
Increasing ANCA titers do not reliably predict relapse.
Nevertheless, many clinicians assume that ANCA titers are reliable indicators of disease activity !.
ANCA\Clinical Applications of Antineutrophil Cytoplasmic Antibody.mht
![Page 25: ANCA Anti-neutrophil cytoplasmic antibodies (ANCAs)](https://reader034.vdocuments.us/reader034/viewer/2022052523/55506e5cb4c90524138b49a5/html5/thumbnails/25.jpg)
Thank You!
www.youtube.com
Histopathology Lung --Wegener granulomatosis - icyou health videos.flv
Mark Lesko's Story - Wegener's Granulomatosis.flv