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SEM Por(olio
Alex Kuhn 11 December 2014
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Part I: Technique DemonstraAon
1. CriAcal Point Drying 2. Depth of Field 3. BackscaMer 4. Low Voltage Image of an Uncoated Sample 5. High MagnificaAon 6. Stereo Pair 7. Cryofracture 8. AestheAc
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Figure 1: Micrograph of an oyster mushroom gill that was prepared by criAcal point drying. OA 2, SS 12, WD 10mm, AV 10 kV.
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Figure 2: DemonstraAon of depth of field in SEM. OA 1, SS 16, WD 32 mm, AV 5 kV.
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Figure 3: a) Secondary electron micrograph of yarn in SEM. b) BackscaMer electron micrograph of (a). OA 2, SS 16, WD 23 mm, AV 5 kV for both images.
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Figure 4: Uncoated fingernail in SEM. OA 1, SS 8, WD 33 mm, AV 2 kV.
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Figure 5:Toothbrush bristle in SEM. OA 1, SS 8, WD 11 mm, AV 30 kV.
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Figure 6: Honey bee head in SEM. OA 2, SS 12, WD 22 mm, AV 10 kV.
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Figure 7: Oyster mushroom gill in SEM. Sample was prepared with criAcal point drying and freeze-‐fractured using liquid nitrogen. OA 1, SS 14, WD 31 mm, AV 10 kV.
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Figure 8: Spore aMached to yarn viewed in SEM. OA 1, SS 12, WD 29 mm, AV 12 kV.
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Part II: PublicaAon Micrographs 1. Biological Samples
Using Photoshop, I adjusted images of a pollen grain and oyster mushroom gills for gamma, contrast, brightness, and unsharp mask. I chose to take micrographs of mushroom gills because I have a strong interest in fungal ecology. I like the image of the pollen grain because it’s stuck on an insect, exemplifying the ubiquitous nature of pollen. The insect was air dried and spuMer coated with Au-‐Pd. The gills were fixed with glutaraldehyde and stained with osmium tetroxide before also being spuMer coated with Au-‐Pd.
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Figure 9: Pollen grain on an insect viewed in SEM. OA 2, SS 16, WD 13 mm, AV 12 kV.
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Figure 10: Freeze fractured mushroom gill viewed in SEM. OA 1, SS 14, WD 31 mm, AV 10 kV.
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Figure 11: Mycelium of an oyster mushroom viewed in SEM. OA 2, SS 12, WD 10 mm, AV 10 kV.
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Figure 12: Hymenium of an oyster mushroom viewed in SEM. OA 1, SS 16, WD 32 mm, AV 5 kV.
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Part II: PublicaAon Micrographs
2. Non-‐biological Samples
The following images are of yarn that was spuMer coated with Au-‐Pd. I chose to take micrographs of yarn because of my interest in knidng. On a macro-‐scale, yarn is so organized and clean. I was curious to see if this changed on the micro-‐scale. I found that, in some ways, it did change-‐ things got more chaoAc. However, in some ways, it remained the same-‐ there are similar paMerns of conAnuity within a single thread as there are in a knit sweater.
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Figure 13: Yarn and spore viewed in SEM. OA 1, SS 12, WD 29 mm, AV 12 kV.
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Figure 14: ParAcle aMached to yarn viewed in SEM. OA 2, SS 10, WD 23 mm, AV 5 kV.
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Figure 15: Cut end of yarn viewed in SEM. OA 2, SS 10, WD 23 mm, AV 5 kV.
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Figure 16: Cut ends of yarn viewed in SEM. OA 2, SS 14, WD 23 mm, AV 5 kV.