Total Integrated Liquid Handling
Air Displacement Pipetting Modules
®
Intelligent Liquid Handling
For Better Results
MOVING LIQUID IS EASY; PRECISION LIQUID
HANDLING IS HARDInstrument manufacturers frequently underestimate the scope of their
liquid handling needs which results in cost overruns, product redesign, and delayed product launch. In an industry where state-of-the-art changes
rapidly, speed to market means the difference between market leadership and a failed product launch. Let us show you how our ZEUS® modules
can get your project done on time and on budget.
Typical pipetting components and their manufacturers place the burden of precision and accuracy on the integrator. Fortunately, the ZEUS is not like any other
pipetting channel on the market and Hamilton is not like any other partner. Hamilton takes ownership of the pipetting process and has developed
an air displacement pipetting module based on the industry leading pipetting technology in our STAR™ liquid handler.
MORE THAN JUST A COMPONENT
Features ZEUS ZEUS LT Competitive Models
Pipette Drive
Pressure Sensor
Capacitive Sensor with Conductive Tips
Tip Ejector
Tip Sensor
Qualitative Pipette Monitoring
Liquid Class Definitions
Z-Axis
Liquid Level Identification
Aspiration and Dispense Tip Following
Container Geometry Definitions
COMES STANDARD OPT IONAL NOT AVAIL ABLE
Excellence Comes Standard
ZEUS LT
ZEUS
Pipetting channel including all critical pipetting functions and quick mounting hardware for connection to an existing Z-Axis.
Pipetting channel with integrated Z-Axis includes aspirate and dispense algorithms that coordinate movements, liquid classes, container geometries, and error handling.
3
Obtaining Accuracy through Air Displacement PipettingWHAT IS AIR DISPLACEMENT PIPETTING?Air displacement pipetting uses a piston to aspirate liquid into a disposable plastic tip. As the piston moves up, the air pressure in the tip is lowered and sample is pushed into the tip by the atmospheric pressure. With this method of pipetting, the sample only contacts the pipette tip which can be discarded between samples to eliminate cross contamination. ZEUS uses a solid state piston that improves performance and minimizes maintenance when compared to liquid filled air displacement systems.
PISTON
DISPOSABLETIP
SAMPLE
1. PISTON MOVES UP
2. A IR PRESSURE LOWERS ASPIR ATING L IQUID
3. T IP IS LOWERED AS L IQUID IS DR AWN INTO THE T IP
4
WHAT ARE THE CHALLENGES WITH AIR DISPLACEMENT PIPETTING? 1
2
3
Plunging the tip to a set height can overflow the vessel.
VESSEL OVERFLOW1
Inconsistent insertion of the tip results in excess liquid on the outside and impacts accuracy.
LIQUID FROM OUTSIDE OF THE TIP
3
Submerging the tip results in force, pushing liquid into the tip even when the plunger does not move.
OVER ASPIRATION2
Air is compressible. This creates challenges and makes achieving acceptable pipetting accuracy and precision difficult if all the variables are not considered and properly compensated for. Characteristics like density, surface tension, volatility, viscosity, and more all have an impact on pipetting accuracy. As seen below, aspirating from the surface of the liquid can also be an important consideration. Thankfully, ZEUS is designed to do all the thinking. Just enter the parameters and ZEUS handles the rest. If the proper parameters are unknown, Hamilton can even help with generating and validating liquid classes.
Not all samples are received with the same volume of liquid in the vessel. Pipetting from
a fixed height can result in significant inaccuracies. While Liquid Level
Detection (LLD) is common across pipetting components, ZEUS is
the only device that can actually find the surface of the liquid
by combining LLD with movement of the Z-Axis. Simple pipetting modules leave this up to the integrator which delays development timelines.
COMMON LLD ISSUES
WHY IS FINDING THE SURFACE OF THE LIQUID CRITICAL?
Liquid Level Detection
LLD requires a precise coordination between the pipette module and the Z-drive. Some pipette components claim LLD capabilities
when in reality all they provide is the raw capacitance or pressure output and the integrator is left with the real work. With simple high
level commands, ZEUS finds the surface of the liquid and positions the pipette accordingly, using capacitance (cLLD) for ionic solutions and a
pressure-based (pLLD) method for non-ionic solutions.5
Intelligent Liquid Handling by Design
ZEUS LT
POWER, RS-232, OR CAN COMMUNICATION, PRESSURE, AND CAPACITANCE S IGNAL
PIPE T T ING DRIVE
PIPE T T ING MODULE WITH ON-BOARD INTELL IGENCE
TIP SENSING EJECT SLEE VE
DISPOSABLE CO-RE PIPE T TE T IP
ZEUS LT BENEFITS & FEATURES
■ No carryover and cross-contamination: ZEUS uses CO-RE disposable tips for pipetting over a large volume range
■ ZEUS LT comes equipped with Qualitative Pipette Monitoring™, a tip presence sensor and both capacitive and pressure liquid level detection
■ Simplified component integration and decreased time to market
■ Reduced instrument maintenance and service with no valves, syringes or tubing required
What is ZEUS?ZEUS is a family of pipetting modules designed for integration into instrumentation requiring on-board pipetting. They perform single sample transfers and multiple dispenses (e.g., aliquoting) from 1 μL to 1 mL with the highest precision and accuracy. The ZEUS Pipetting Modules utilize Hamilton’s revolutionary air displacement pipetting technology and CO-RE™ (compressed O-ring expansion) tips.
6
2 STRAIGHT CONFIGURATION1
FOLDED CONFIGURATION2
RIGHT ANGLE CONFIGURATION3
3
ALTERNATIVE Z-AXIS CONFIGURATIONS
ZEUS is available in a variety of configurations to meet the needs of your instrument. To the right are a few examples of ZEUS configurations viewed from the top.
ZEUS
POWER, AND CAN COMMUNICATION
PIPE T T ING DRIVE
Z-DRIVE
PIPE T T ING MODULE WITH ON-BOARD INTELL IGENCE
TIP SENSING EJECT SLEE VE
DISPOSABLE CO-RE PIPE T TE T IP
ZEUS WITH INTEGRATED Z-AXISADDITIONAL BENEFITS & FEATURES
■ High level aspirate and dispense commands use liquid classes to synchronize pipette and Z-Axis movements to find the surface of the liquid and follow the liquid during pipetting according to the defined container geometry
■ The Z-Axis is strong enough to pierce foil or septa seals but delicate enough to detect a foil surface without leaving a mark
■ High resolution Z-Axis (0.1 mm) makes it possible to automatically teach critical deck locations with no operator interaction
■ Validated pipetting algorithms mean less time spent on pipetting and quicker product launches
INTEGR ATEDZ-A X IS
1
7
Pipetting PerformanceGuaranteed
ZEUS has a small pressure sensor integrated into the pipetting channel. This monitors the pressure in the air space between the piston and the liquid. By correlating the pressure curve with the movement of the piston a standard curve for successful aspiration or dispensing can be established and deviations from this standard that affect the accuracy and precision can be detected. This process is known as Qualitative Pipette Monitoring.
WHAT DOES SUCCESSFUL PIPETTING LOOK LIKE?
On the STAR Liquid Handling Platform Hamilton pioneered the art of translating pressure curves into intelligent actions. With ZEUS, the benefit of this knowledge is available for integration into any liquid handling application. Some pipetting modules are capable of reporting pressure data, but ZEUS is the only one capable of interpreting the data and providing actionable information back to the system.
QUALITATIVE PIPETTE MONITORING (QPM)
SUCCESSFUL DISPENSE
SUCCESSFUL ASPIRATION P
RESSURE
TIME
PRESSURE SENSOR
8
Volatile liquids like acetone and methanol can be extremely difficult to pipette using air displacement. These liquids tend to vaporize in the tip which raises the air pressure resulting in liquid dripping from the tip. ZEUS is equipped with Anti-Droplet Control (ADC). When enabled, the pressure is monitored in the tip. As the pressure rises, the piston is retracted to equalize the pressure and prevent dripping. The illustrations show the pressure curve when ADC is on and off.
PIPETTING VOLATILE LIQUIDS
CLOGGED TIP DETECTION
If the tip clogs during aspiration the pressure will spike down. As the plunger moves, sample is not being aspirated to help equalize the pressure.
INSUFFICIENT LIQUID DETECTION
If there is insufficient liquid in the tube, the pressure curve will start down as normal but then will return to a pressure much higher than the expected level represented by Δp in the graph. The higher pressure is a result of having less liquid in the tip due to an incomplete aspiration.
ASPIRATION OF FOAM
If ZEUS pipettes foam instead of liquid, the pressure sensor detects spikes during the aspiration. Based on the severity of the spikes, a threshold can be set for a failed aspiration.
WHAT TYPES OF PIPETTING ERRORS ARE DETECTED BY QPM?Below are some examples of how the pressure curves for various common pipetting errors will look relative to a successful aspiration.
EMPTY TUBE THRESHOLD
EMPTY TUBE
SUCCESSFUL ASPIRATION
∆pMAX
PR
ES
SU
RE
T IME
TIP CLOGGED
CLOT THRESHOLD
SUCCESSFUL ASPIRATION
∆pMAX
PR
ES
SU
RE
T IME
FOAM ASPIRATION
SUCCESSFUL ASPIRATION
PLUNGERMOVEMENTSTOPS
PR
ES
SU
RE
T IME
Pressure reaches a maximum; a droplet is lost and pressure drops.
Piston retracts when pressure threshold is reached so no droplet forms.
PR
ES
SU
RE
T IME
PR
ES
SU
RE
T IME
ADC ON
VAPOR
LIQUID
ADC OFF
9
We Have a Tip for ThatHamilton offers a wide range of pipette tips to solve a variety of common automation challenges. Conductive, non- conductive, slim and piercing tips are available in a variety of different volumes and packaging options. Below is an example of some off the shelf tips. Contact Hamilton to discuss which tips are most appropriate for your application.
Standard Pipette Tips
Tip Volume
Description 10 μL 50 μL 150 μL* 250 μL* 300 μL 1 mL
Conductive
Conductive, Sterile
Conductive, Filtered
Conductive, Filtered, Sterile
Conductive, Stacked
Conductive, Stacked, Sterile
Wide Bore, Conductive
Slim Tip Conductive
Slim Tip, Conductive, Filtered
Non-Conductive, Clear stacked
*150 µL and 250 µL tip volumes are septum piercing design.
ZEUS Accuracy & Precision By Tip Size
Tip Volume Dispense Volume Accuracy Precision
10 µL 1 µL 5.0% 4.0%
10 µL 5 µL 2.5% 1.5%
10 µL 10 µL 1.5% 1.0%
10 µL 1 µL 5.0% 4.0%
50 µL 5 µL 2.5% 1.5%
50 µL 50 µL 2.0% 0.75%
300 µL 10 µL 5.0% 2.0%
300 µL 50 µL 2.0% 0.75%
300 µL 300 µL 1.0% 0.75%
1000 µL 10 µL 7.5% 3.5%
1000 µL 100 µL 2.0% 0.75%
1000 µL 1000 µL 1.0% 0.75%
* Pipetting specifications were determined gravimetrically using a high precision balance and strictly controlled environmental conditions: test temperature: 20 ± 2°C, relative humidity: 50% ± 5%, test liquid temperature range: ≤± 0.5°C of room temperature, balance used: Mettler Toledo MX5. The measurements were done in Hamilton Verification Solution (deionized water with 0.1% NaCl and 0.01% Tween detergent) using standard CO-RE conductive tips. A new tip was used for each pipetting cycle (aspiration and dispense). At least 10 points were taken for each volume and pipetting module. Volumes > 20 μL were dispensed in jet mode. Volumes ≤ 20 μL were dispensed in surface mode. Results may vary using other liquid or environmental conditions.
10
11
Hamilton Americas & Pacific RimHamilton Company Inc.4970 Energy WayReno, Nevada 89502 USATel: +1-775-858-3000Fax: [email protected]
Hamilton Europe, Asia, & AfricaHamilton Bonaduz A.G.Via Crusch 8CH-7402 Bonaduz, SwitzerlandTel: +41-58-610-10-10 Fax: +41-58-610-00-10 [email protected]
Web: www.hamiltoncompany.comUSA: 800-648-5950Europe: +41-58-610-10-10 To find a representative in your area, please visit hamiltoncompany.com/contacts.
©2017 Hamilton Company. All rights reserved. All trademarks are owned and/or registered by Hamilton Company in the U.S. and/or other countries.
Lit. No. L60037 Rev. B – 07/2017
Intelligent liquid handling for best results ZEUS (Z-Excursion Universal Sampler™) has been designed to take ownership of the entire pipetting process. It is based on Hamilton’s revolutionary air displace-ment pipetting technology and uses dis-posable CO-RE® tips to avoid carry-over.
With its integrated z-motion, ZEUS auto-matically picks up the tip, detects and follows the liquid level during aspiration and dispense. ZEUS adjusts its move-ments to the liquid type to ensure accu-rate and precise pipetting. The on-board Qualitative Pipetting Monitoring™ (QPM) actively prevents malfunctions. These high level capabilities allow customers to significantly shorten development time-lines and get product to market faster.
ZEUS® Pipetting ModuleSpecification Sheet
Side Front
Plot Abs.: mbuehler 24.06.2013 13:49:32 Plot Abs.: mbuehler 24.06.2013 13:49:32
Top Bottom
Plot Abs.: mbuehler 24.06.2013 13:49:32
Plot Abs.: mbuehler 24.06.2013 13:49:32
ZEUS Product Drawings and Dimensions in mmStraight Configuration
Disposable CO-RE Tips*Part Number Description
235904-ZEUS 1000 µL tip non-filtered conductive 40 racks of 96
235905-ZEUS 1000 µL tip filtered conductive 40 racks of 96
235902-ZEUS 300 µL tip non-filtered conductive 60 racks of 96
235903-ZEUS 300 µL tip filtered conductive 60 racks of 96
235806-ZEUS 300 µL slim tip non-filtered conductive 40 racks of 96
235647-ZEUS 300 µL slim tip filtered conductive 40 racks of 96
235966-ZEUS 50 µL tip non-filtered conductive 60 racks of 96
235948-ZEUS 50 µL tip filtered conductive 60 racks of 96
235900-ZEUS 10 µL tip non-filtered conductive 60 racks of 96
235901-ZEUS 10 µL tip filtered conductive 60 racks of 96
*Other tips are available on request
Ordering Information
Tip ejection sleeveCO-RE LT adapter Holder for CO-RE tip rack
ZEUS Pipetting ModulePart Number Description Includes
800700 ZEUS CO-RE module
202977-0020 Test platform Holder for the ZEUS module, and Communication box
202977-0021 Holder for ZEUS module
202977-0022 Communication box USB / Hamilton CAN converter, power supply, cables and software CD
Accessories and Spare PartsPart Number Description
800336 Holder for CO-RE tip rack
800337 Tip ejection sleeve (1/pk)
800952 CO-RE LT adapter (1/pk)
Test platform
CERTIFIED
DNase-/RNase-free
Biological purity tested
non-pyrogenic
96 x 1000 μL CO-RE tips
For more information on ZEUS and other Hamilton OEM Components please visit
www.hamiltoncompany.com/OEM
Product SpecificationsDimensions 25 mm x 205 mm x 337 mm 45 mm x 118 mm x 337 mm 161 mm x 102 mm x 337 mm Width x Depth x Height
Straight configurations Folded configurations Right angle configuration (Single or parallel arrangement) (Single arrangement or as a pair) (Single arrangement)
*Pipetting specifications were determined gravimetrically using a high precision balance and strictly controlled environmental conditions: test temperature: 20 ± 2°C, relative humidity: 50% ± 5%, test liquid temperature range: ≤± 0.5°C of room temperature; balance used: Mettler Toledo MX5.
The measurements were done with Hamilton Verification Solution (deionized water with 0.1% NaCl and 0.01% Tween detergent) using standard CO-RE conductive tips. A new tip was used for each pipetting cycle (aspiration and dispense). At least 10 points were taken for each volume and pipetting module. Volumes > 20 μL were dispensed in jet mode. Volumes ≤ 20μL were dispensed in surface mode. Results may vary using other liquid or environmental conditions.
Pipetting Performance*Disposable Tip Size Pipetting Volume Accuracy Precision
10 μL 1 μL 5.0% 4.0%
10 μL 5 μL 2.5% 1.5%
10 μL 10 μL 1.5% 1.0%
50 μL 1 μL 5.0% 4.0%
50 μL 5 μL 2.5% 1.5%
50 μL 50 μL 2.0% 0.75%
300 μL 10 μL 5.0% 2.0%
300 μL 50 μL 2.0% 0.75%
300 μL 300 μL 1.0% 0.75%
1000 μL 10 μL 7.5% 3.5%
1000 μL 100 μL 2.0% 0.75%
1000 μL 1000 μL 1.0% 0.75%
Weight 1100 g
z-Drive Maximum z-Excursion 180 mm Resolution z-Axis 0.1 mm Maximum Speed z-Axis 350 mm/s
Pipetting Drive Volume Range 1 μL – 1000 μL Resolution 0.1 μL Maximum Speed 150 mm/s corresponding to 2’500 μL/s
Pipetting Mode Single and aliquot dispensing
Process Security Tip presence sensor Pressure-based and capacitive Liquid Level Detection (cLLD and pLLD) Qualitative Pipetting Monitoring (QPM) Anti-Droplet Control (ADC)
Operating Data Power requirement 48 VDC ± 5%, 1.8 A Temperature range 18°C – 32°C Relative humidity 40% – 80% (non-condensing) Noise level < 60 dBA, Indoor use only
Storage and Transportation Temperature range -25°C to +70°C Relative humidity 40% – 80% (non-condensing, indoors)
Communication CAN (Hamilton protocol), FCC connector Selectable baud rates 125, 500 kBaud (others on request) Device addressing up to 31 modules on a single CAN bus
Hamilton Americas & Pacific RimHamilton Company Inc.4970 Energy WayReno, Nevada 89502 USATel: +1-775-858-3000Fax: [email protected]
Hamilton Europe, Asia & AfricaHamilton Central Europe S.R.L.str. Hamilton no. 2-4307210 Giarmata, RomaniaTel: +40-356-635-055Fax: [email protected]
Web: www.hamiltoncompany.comUSA: 800-648-5950Europe: +40-356-635-055 To find a representative in your area, please visit hamiltoncompany.com.
© 2017 Hamilton Company. All rights reserved. All trademarks are owned and/or registered by Hamilton Company in the U.S. and/or other countries.
Lit. No. 608770/04 — 11/2017
18 mm
18 mm
18 mm
18 mm
ZEUS LT is the lite version of Hamilton’s industry leading OEM pipetting module ZEUS.
The module enables OEM customers to incorporate a wide range of intelligent pipetting features into their instruments. The strongly reduced size allows an easy integration even for small instruments where space is a crucial criterion. Qualitative Pipetting Monitoring™ (QPM) or on-board liquid level detection are essential requirements for manufacturers of analytical instruments and clinical diagnostic systems. ZEUS LT captures these demands while allowing the freedom to operate with an individual z-axis.
This makes ZEUS LT the ideal candidate for instrument developers who do not require ZEUS’ integrated z-axis.
ZEUS® LT Pipetting Module Specification Sheet
ACCURATE MODULE MOUNTING
TIP PRESENCE SENSOR
ANTI-DROPLET CONTROL (ADC™)
RS-232 AND CAN COMMUNICATION
STATUS LED
AUTOMATED TIP EJECTOR
WIDE VOLUME RANGE OF DISPOSABLE TIPS
QUALITATIVE PIPETTE MONITORING™
ON-BOARD CAPACITIVE AND PRESSURE LLD
C
88,5 0,5
min
305
with
9,
5
min
326
with
9,
5
min
335
with
9,
5
min
371
with
9,
5
12 (12x)
8 (3
x)
35
70
8 (3
x)
35
70
1 (3
x)
M2 (12x)
3,1 (3x)
12
26
35
7015
,5
2,2 (1
2x)
4,2 (12x)
5
2,5 (12x)
238,
2
282,
9
100
17
1000
µL T
ip
300µ
L S
lim T
ip
300µ
L Ti
p
50µL
Tip
10µL
Tip
reference surface
2/1
3 holes for positioningpins with 3 h6
ground contact
ZEUS LT Product Drawings and Dimensions (in mm)
Side Front
C
88,5 0,5
min
305
with
9,
5
min
326
with
9,
5
min
335
with
9,
5
min
371
with
9,
5
12 (12x)
8 (3
x)
35
70
8 (3
x)
35
70
1 (3
x)
M2 (12x)
3,1 (3x)
12
26
35
7015
,5
2,2 (1
2x)
4,2 (12x)
5
2,5 (12x)
238,
2
282,
9
100
17
1000
µL T
ip
300µ
L S
lim T
ip
300µ
L Ti
p
50µL
Tip
10µL
Tip
reference surface
2/1
3 holes for positioningpins with 3 h6
ground contact
C
88,5 0,5
min
305
with
9,
5
min
326
with
9,
5
min
335
with
9,
5
min
371
with
9,
5
12 (12x)
8 (3
x)
35
70
8 (3
x)
35
70
1 (3
x)
M2 (12x)
3,1 (3x)
12
26
35
7015
,5
2,2 (1
2x)
4,2 (12x)
5
2,5 (12x)
238,
2
282,
9
100
17
1000
µL T
ip
300µ
L S
lim T
ip
300µ
L Ti
p
50µL
Tip
10µL
Tip
reference surface
2/1
3 holes for positioningpins with 3 h6
ground contact
Bottom
Ordering Information
ZEUS LT Pipetting Module
Part Number Description
800888 ZEUS LT CO-RE module
800873 Demo cable for RS232
Accessories and Spare Parts
Part Number Description
800336 Holder for CO-RE tip rack
800337 Tip ejection sleeve CO-RE (1/pk)
800952 CO-RE LT adapter (1/pk)
Disposable CO-RE Tips*
Part Number Description
235904-ZEUS 1000 µL tip non-filtered conductive 40 racks of 96
235905-ZEUS 1000 µL tip filtered conductive 40 racks of 96
235902-ZEUS 300 µL tip non-filtered conductive 60 racks of 96
235903-ZEUS 300 µL tip filtered conductive 60 racks of 96
235806-ZEUS 300 µL slim tip non-filtered conductive 40 racks of 96
235647-ZEUS 300 µL slim tip filtered conductive 40 racks of 96
235966-ZEUS 50 µL tip non-filtered conductive 60 racks of 96
235948-ZEUS 50 µL tip filtered conductive 60 racks of 96
235900-ZEUS 10 µL tip non-filtered conductive 60 racks of 96
235901-ZEUS 10 µL tip filtered conductive 60 racks of 96
*Other tips are available on request.
CO-RE LT adapter
Holder for CO-RE tip rack
For more information on ZEUS LT and other Hamilton OEM Components please visit
www.hamiltoncompany.com/OEM
Tip ejection sleeve
CERTIFIED
DNase-/RNase-free
Biological purity tested
non-pyrogenic
96 x 1000 μL CO-RE tips
Hamilton Americas & Pacific Rim4970 Energy WayReno, Nevada 89502 USATel: +1-775-858-3000Fax: [email protected]
Hamilton Europe, Asia & AfricaVia Crusch 8CH-7402 Bonaduz, SwitzerlandTel: +41-58-610-10-10Fax: [email protected]
Web: www.hamiltoncompany.comUSA: 800-648-5950Europe: +41-58-610-10-10 To find a representative in your area, please visit www.hamiltoncompany.com.
Specifications
Dimensions Width x Depth x Height 17 x 100 x 283 mm
Weight 410 g
Pipetting Drive Volume Range 1 µL – 1’000 µL
Resolution 0.1 µL
Maximum Speed 1’500 µL/s
Pipetting Mode Single and Aliquot Dispensing
Process Security Tip Presence Sensor
Pressure-based and Capacitive Liquid Level Detection (pLLD and cLLD)
On board Qualitative Pipetting Monitoring (QPM)
Anti-Droplet Control (ADC)
Operating Data Power Requirement 24 VDC ± 5%, 200 mA
Temperature Range 18°C – 32°C
Relative Humidity 40% – 80% (non-condensing)
Noise Level < 60 dBA
Indoor use only
Storage and Transportation Temperature Range -25°C ± 70°C
Relative humidity 40% - 80% (non-condensing, indoors)
Communication RS232 Full duplex, STX ETX or CR-LF protocol Selectable baud rate (19’200 or 38’400)
CAN Hamilton protocol
Device Addressing Up to 31 modules in single CAN bus Up to 32 modules with RS232
Pipetting Performance*
Disposable Tip Size Pipetting Volume Accuracy [A] Precision CV
10 μL 1 μL 5.0% 4.0%
10 μL 5 μL 2.5% 1.5%
10 μL 10 μL 1.5% 1.0%
50 μL 1 μL 5.0% 4.0%
50 μL 5 μL 2.5% 1.5%
50 μL 50 μL 2.0% 0.75%
300 μL 10 μL 5.0% 2.0%
300 μL 50 μL 2.0% 0.75%
300 μL 300 μL 1.0% 0.75%
1000 μL 10 μL 7.5% 3.5%
1000 μL 100 μL 2.0% 0.75%
1000 μL 1000 μL 1.0% 0.75%
© 2016 Hamilton Company. All rights reserved.
Lit. No. 695216/00 — 11/2016
*Pipetting specifications were determined gravimetrically using a high precision balance and strictly controlled environmental conditions: test temperature: 20°C ± 2°C, relative humidity: 50% ± 5%, test liquid temperature range: ≤ ± 0.5°C of room temperature, balance used: Mettler Toledo MX5. The measurements were done with Hamilton Verification Solution (deionized water with 0.1% NaCl and 0.01% Tween detergent) using standard CO-RE conductive tips. A new tip was used for each pipetting cycle (aspiration and dispense). At least 10 points were taken for each volume and pipetting module. Volumes > 20 μL were dispensed in jet mode. Volumes ≤ 20 μL were dispensed in surface mode. Results may vary using other liquid or environmental conditions. Results may also vary with incorrect usage of the z-axis.