Download - Additional figure 1 Ballini et al
Additional figure 1 Ballini et al
0N 1N 0N 1N 0N 1NSL4- CD203 SL4- CL32 SL4- Guy11
0N 1N 0N 1N 0N 1NSL4- TH16 SL4- US31 SL4-VT2
0N 1N 0N 1N 0N 1NSL5- CD203 SL5- CL32 SL5- Guy11
0N 1N 0N 1N 0N 1NSL5- TH16 SL5- US31 SL5-VT2
0N 1N 0N 1N 0N 1NSL19- CD203 SL19- CL32 SL19- Guy11
0N 1N 0N 1N 0N 1NSL19- TH16 SL19- US31 SL19-VT2
0N 1N 0N 1N 0N 1NKasalath-CD203 Kasalath-CL32 Kasalath-Guy11
0N 1N 0N 1N 0N 1NKasalath-TH16 Kasalath-US31 Kasalath-VT2
0N 1N 0N 1N 0N 1NNipponbare-CD203 Nipponbare-CL32 Nipponbare-Guy11
0N 1N 0N 1N 0N 1NNipponbare-TH16 Nipponbare-US31 Nipponbare-VT2
CD20
3CL
32GU
Y11
TH16
US31 VT
2CD
203
CL32
GUY1
1TH
16US
31 VT2
CD20
3CL
32GU
Y11
TH16
US31 VT
2CD
203
CL32
GUY1
1TH
16US
31 VT2
CD20
3CL
32GU
Y11
TH16
US31 VT
2
Nipponbare Kasalath CSSL4 CSSL5 CSSL19
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
0N 1N
Num
ber o
f sus
cepti
ble
lesio
n/le
af su
rfac
e ar
eaAdditional figure 2 Ballini et al
****
** *
**
** **
***
***
******
******
***
***
******
***
*** **
*
Additional Table 1 NIS across rice and M. oryzae diversity
Guy1
1CD
203
CL26
Nip
ponb
are
tem
pera
te ja
poni
caRo
bust
par
tial R
esis
tanc
eRo
bust
par
tial R
esis
tanc
eRo
bust
par
tial R
esis
tanc
eIR
64in
dica
Robu
st co
mpl
ete
Resi
stan
ceRo
bust
com
plet
e Re
sist
ance
Robu
st co
mpl
ete
Resi
stan
ceTr
embe
setr
opic
al ja
poni
caM
ore
susc
eptib
le w
ith N
itrog
enM
ore
susc
eptib
le w
ith N
itrog
enRo
bust
par
tial R
esis
tanc
eIR
8in
dica
Robu
st co
mpl
ete
Resi
stan
ceRo
bust
com
plet
e Re
sist
ance
Mor
e su
scep
tible
with
Nitr
ogen
Mor
ober
eken
trop
ical
japo
nica
Robu
st p
artia
l Res
ista
nce
Robu
st p
artia
l Res
ista
nce
Robu
st p
artia
l Res
ista
nce
Pao
Tou
Hung
indi
caM
ore
susc
eptib
le w
ith N
itrog
enM
ore
susc
eptib
le w
ith N
itrog
enRo
bust
par
tial R
esis
tanc
ePe
tita
jang
gut
indi
caM
ore
susc
eptib
le w
ith N
itrog
enna
Mor
e su
scep
tible
with
Nitr
ogen
Ta M
ao T
aote
mpe
rate
japo
nica
Mor
e su
scep
tible
with
Nitr
ogen
naM
ore
susc
eptib
le w
ith N
itrog
enAz
ucen
atr
opic
al ja
poni
caM
ore
susc
eptib
le w
ith N
itrog
enna
Robu
st p
artia
l Res
ista
nce
Mar
atel
lite
mpe
rate
japo
nica
Mor
e su
scep
tible
with
Nitr
ogen
naM
ore
susc
eptib
le w
ith N
itrog
enKa
sala
thAu
sM
ore
susc
eptib
le w
ith N
itrog
enM
ore
susc
eptib
le w
ith N
itrog
enM
ore
susc
eptib
le w
ith N
itrog
enCh
uan
4te
mpe
rate
japo
nica
Mor
e su
scep
tible
with
Nitr
ogen
Mor
e su
scep
tible
with
Nitr
ogen
Mor
e su
scep
tible
with
Nitr
ogen
Popo
t 165
indi
caM
ore
susc
eptib
le w
ith N
itrog
enM
ore
susc
eptib
le w
ith N
itrog
enM
ore
susc
eptib
le w
ith N
itrog
enPa
di B
oeno
rin
dica
Mor
e su
scep
tible
with
Nitr
ogen
Mor
e su
scep
tible
with
Nitr
ogen
Mor
e su
scep
tible
with
Nitr
ogen
Mag
napo
rthe
ory
zae
isol
ate
rice accession
Additional Table 2 Recombinant lines establishing NIS1 on chromosome 1
11
24
44
49
RM11
219
RM59
31
RM40
6 RM
551
RM16
502
RM24
1 RM
3335
RM
2386
5 Ph
enot
ype
unde
r high
N4-
40-7
ka
s ka
s N
ip
Nip
N
ip
Nip
N
ip
Nip
M
ore
Susc
eptib
le4-
40-8
ka
s ka
s N
ip
Nip
N
ip
Nip
N
ip
Nip
M
ore
Susc
eptib
le4-
40-2
0 ka
s ka
s ka
s ka
s ka
s N
ip
Nip
N
ip
Mor
e Su
scep
tible
19-2
4 ka
s ka
s N
ip
Nip
N
ip
Nip
N
ip
Nip
M
ore
Susc
eptib
le
lines
chro
mos
ome
and
micr
osat
telite
mar
kers
Additional Table 3 Further mapping of the NIS1 locus
More susceptible in 1N No difference in 1N and 0N
Marqueur Position (Mbp) CSSL4 CSSL5 CSSL19 19-42-4 19-42-2 CSSL9
RM7075 15.12 Nip Nip Kas Kas Kas KasRM11007 17.95 Nip Nip Kas Kas Kas KasRM1196 21.90 Kas Nip Kas nd nd ndRM11219 22.16 Kas Nip Kas Nip Nip ndRM5718 22.93 Kas Nip Kas Nip Nip NipRM6716 23.12 Kas Nip Nip Nip Nip NipRM2318 24.14 Kas Nip Nip Nip Nip NipRM3440 27.19 Kas Kas Nip Nip Nip NipRM7419 27.70 Kas Nip Nip Nip Nip ndRM7566 27.77 Kas nd Kas Kas Nip ndRM11522 28.07 Kas Kas Kas Kas Nip NipRM11504 28.11 Kas Kas Kas Kas Nip NipRM11563 29.16 Kas Kas Kas Kas Nip NipRM5931 29.28 Kas Kas Kas Kas Nip NipRM11581 29.46 Kas Kas Kas Kas Nip NipRM11594 29.62 Kas Kas Kas Kas Nip NipRM6702 29.77 Kas Kas Kas Kas Nip NipRM3615 29.81 Kas Kas Kas Kas Nip NipRM1152 30.09 Kas Kas Kas Nip Nip ndRM11631 30.52 Kas Kas Kas Kas Nip ndRM11646 30.71 Kas Kas Kas Kas Nip ndRM1095 30.92 Kas Kas Kas Nip Nip ndRM11658 31.04 Nip Kas Kas Nip Nip ndRM8097 31.41 Nip Kas Kas nd nd ndRM11668 31.42 Nip Kas Kas Nip Nip nd
Concentration (mM) elements 0N 1N
Macronutrient
K2SO4 1.34 1.34KH2PO4 1.97 1.97NH4NO3 0.00 0.50MgSO4, 7H2O 1.53 1.53
Micronutrient
H3BO3 0.91 0.91MnSO4 0.50 0.50(NH4)6Mo7O24,4H2O 0.01 0.01CuSO4, 5H2O 0.08 0.08ZnSO4, 7H2O 0.31 0.31
Ferveg (6% Fe) H2O pH 5,5
Additional Table 4 Composition of fertilizing solution
Additional Table 5 Primer sequences for q-PCR experiments
Gene name Annotation Accession Sequence Forward Primer Sequence Reverse PrimerPR5 protein PR (pathogenesis related) Os12g43430 CGCTGCCCCGACGCTTAC ACGACTTGGTAGTTGCTGTTGCPBZ1 protein PR (pathogenesis related) Os12g36880 CCTGCCGAATACGCCTAAGATG AGAACACATTCAGACTTGCCTCTCOsMT2b Os05g02070 ACCGTCGTCGTCGTTGTCAG GCATGAGGAGATGGAGCAGGAGPAL Os02g41650 TTCTTGATCGCCCTGTGCCAAG TGCATCACGCAGTTCTTCACCGlucanase protein PR (pathogenesis related) Os01g71340 CGTTCCATATATAGTTTGGGTTTG TCAAGTTCATATTATTTGTCTCTGSubtilase protein PR (pathogenesis related) Os01g58290 CGGAAAGTTGCTGACCCATTCG GCTCGCCGCACCTTGATTGPR3 protein PR (pathogenesis related) Os04g41620 CGTGTCTGTGGAGAGCGTGGTC TCGTCGTTGGTGCGGTCATTGGPOX223 protein PR (pathogenesis related) Os07g48020 GCTCATCCCCATCAACAACT GTGCACTGGATTGACCAG