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8 seqs/day
96 seqs/2 hrs
Bioinformatics for Genomics
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TAGAGCATCGATCGATGCTGCAGATGATGCTAGCATCGGCTAGGCGACG
ATCTCGTAGCTA
ATCTCGTAGCTAGCTACGACGTCTA
ATCTCGTAGCTAGCTA
ATCTCGTAGCTAGATCTCGTAGCTAGCATCTCGTAGCTAGCT
ATCTCGTAGCTAGCTACATCTCGTAGCTAGCTACGATCTCGTAGCTAGCTACGAATCTCGTAGCTAGCTACGACATCTCGTAGCTAGCTACGACGATCTCGTAGCTAGCTACGACGTATCTCGTAGCTAGCTACGACGTCATCTCGTAGCTAGCTACGACGTCT
ATCTCGTAGCTATCTCGTAGCTATCTCGTAGCTATCTCGTAGCTATCTCGTAGCTATCTCGTAGCTATCTCGTAGCTATCTCGTAGCTATCTCGTAGCTATCTCGTAGCTATCTCGTAGCTATCTCGTAGCTATCTCGTAGCTATCTCGTAGCT
A
G
C
T
A
C
G
A
C
G
T
C
T
A
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20
30
10
• Random base calling at the beggining or the end of read (Phred < 10)• Trimming (trim or trim_alt algorithms)
•Phred does the base calling
chromatogram
acgatctcgctagctgctactgtagccgcgattattcgcgatctacgtatatcgcgatcgatc
• Each base has assigned a chance of failure
• 1% = 0,01 = 10-2 = Phred 20
Base calling & trimming
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Start
End
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Goal: To document the presence of transcripts in a transcriptome [otorrin... in portuguese]
• EST = Expressed Sequence Tag
Partial sequencing of transcripts in EST genome projects
actgatcatctcgctgatgcgatc
work