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1'
Pitahaya'Diseases'
Gary'Bender'Ph.D.'Farm'Advisor'Emeritus'–'Subtropical'
HorEculture'UCCE'San'Diego'County'
Diseases'–'Cause&for&Concern?'
• Pitahaya'imported'into'California,'and'exported'from'California'(to'Hawaii'for'instance)'– Is'it'diseasePfree?'– How'can'we'know'it'is'disease'free?'– Are'we'ruining'our'industry??'
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California'has'a'Huge'Advantage!'In&Pitahaya&Produc4on'
• Our'dry'climate'allows'us'to'grow'this'fruit'without'fungicides'(at'least'unEl'something'new'comes'along)'
• The'tropical'countries'have'to'use'a'lot'of'fungicides,'much'like'avocado'producEon'
• This'is'the'one'and'only'advantage'to'a'prolonged'drought'in'California!'
Diseases'in'California'• Generally,'with'our'dry'
climate,'the'diseases'are'few,'however:'
• Plants'can'be'damaged'by'intense'light,'and'this'damaged'Essue'may'be'suscepEble'to'stem'rot'caused'by'a'bacterium''
• Plants'can'be'damaged'by'too'much'irrigaEon'water,'making'them'suscepEble'to'root'rot'caused'by'several'fungi'
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3'
Disease'Caused'by'Bacteria'• SoX'rots'of'the'stem'and'
blades'are'caused'by'Xanthomonas&campestris&and'Erwinia&carotovora&
• Several&countries&report&these&as&Enterobacteria&cloacae&(2009).&&Are&they&the&same??&
• Calcium'deficiency'in'Mexican'planEngs'has'been'shown'to'make'this'problem'worse'
SoX'Rots'• SoX'stem'rot'caused'by'
Erwinia&caratovora&in'Taiwan'matches'a'stem'rot'found'in'Calif.'
• InfecEon'starts'from'injured'areas'(sunburn,'other'diseases'spots'caused'by'anthracnose)'
• Control'includes'pruning'out'dying'stems'and'spraying'with'copper'sulfate'
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Diseases'Caused'by'Fungi'
• Dothierella'(Botryosphaeria)'• Anthracnose'(Colletotrichum)'• Bipolaris&• Fusarium&• Alternaria&• Phytophthora&
Botryosphaeria'Brown&Stem&Spot&Disesease'
• Botryosphaeria&dothidea&• First'reported'in'Mexico'
in'2003'• The'Fusicoccum&stage'was'
isolated'in'pure'culture,'rePinoculated'and'the'exact'same'symptoms'were'produced'
• Small'chloroEc'specks'which'enlarge'up'to'5'cm'in'diameter'
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8/26/14'
5'
Botryosphaeria'
• Picture'is'from'Thailand'
Botryosphaeria'Disease'Management'
• Prune'off'dead'limbs'and'dispose'away'from'the'planEngs.'Maintain'a'thick'layer'of'mulch'to'hasten'decomposiEon'of'fungi'on'the'ground.'Use'good'sanitaEon'and'opEmal'cultural'pracEces'to'minimize'disease.'
• When'weather'changes'from'cool'to'warm,'appropriately'modify'the'irrigaEon'program,'and'pay'special'abenEon'to'irrigaEon'needs'during'periods'of'hot'weather.'
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Anthracnose'• Colletotrichum&gloeosporioides&• Reported'in'S.'Florida'in'2007'• Reddish'brown'lesions'with'
conspicuous'chloroEc'haloes'developed'concentrically'on'the'edges'of'vine'ribs.''
• Lesion'centers'became'white'and'coalesced'to'rot'much'of'the'vine'column,'and'in'severe'cases,'only'the'vascular'column'in'the'vine'center'was'not'diseased'
– From'Palmateer,'Ploetz,'van'Santen'and'Correll'2007'
''
Management'of'Anthracnose'• Remove'diseased'
branches'• Clean'clippers'with'10%'
bleach'• If'high'rainfall'and'misty'
wet'condiEons,'apply'copper'hydroxide'prior'to'wet'condiEons.''Copper'will'reduce'new'infecEons,'but'will'not'“cure”'an'exisEng'infecEon.'
• Check'the'registraEons!'
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8/26/14'
7'
Bipolaris'Fruit'Rot'• This'rot'begins'as'small'
tan,'circular'lesions'on'the'fruit'surface'and'as'the'diseases'progresses'the'lesions'enlarge'and'turn'black.''
• Usually'a'black'feltPlike'growth'of'the'fungus'can'be'observed'on'the'lesions.'Under'ideal'condiEons'(warm'and'humid),'the'fruit'develop'large'areas'of'soX'rot.'
Bipolaris'fruit'rot'
• The'lesions'are'caused'by'a'fungus,'Bipolaris&cac4vora.'This'species'causes'stem'and'fruit'rot'of'cacE'(including'pitahaya)'in'California,'Florida,'Europe'and'Japan.'
• Former'name'was'Helminthosporium'
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Bipolaris'fruit'rot'• The'disease'is'most'severe'on'mature'and'ripe'fruit.'While'young'stems'are'suscepEble'to'B.#cac&vora,'mature'stems'are'relaEvely'resistant'to'infecEon.'
• Bipolaris&rot'on'ornamental'cactus'is'most'severe'between'75P91'F.'
• 'In'general,'diseases'caused'by'Bipolaris'are'favored'by'humid'condiEons.''
• Inoculum'sources'include'diseased'plants'in'the'field'and'crop'residue.'Conidia'are'most'oXen'spread'by'wind,'irrigaEon'and'rain.'''
Bipolaris'fruit'rot'P'Florida'• Small,'circular,'light'brown,'depressed'lesions'expanded'to'form'large'areas'of'rot'on'flowers'and'fruit'in'7'to'10'days.''
• These'lesions'produced'large'amounts'of'dark'fungal'spores.'
• On'mature'plant'stems,'woundPinoculated'treatments'formed'1.8'to'3.4'mm'lesions,'but'nonwounded'inoculaEons'and'controls'were'negaEve.'Lesions'were'light'tan,'circular,'and'did'not'sporulate.'– T.'L.'B.'Tarnowski,'A.'J.'Palmateer,'and'J.'H.'Crane,'University'of'Florida,'Tropical'Research'and'EducaEon'Center,'Homestead'33031P3314''
''
''
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8/26/14'
9'
Bipolaris'fruit'rot'
• “The'high'incidence'of'fruit'rot'affecEng'commercial'operaEons'in'MiamiPDade'County'over'the'past'2'years'requires'an'effecEve'disease'management'strategy.”'– T.'L.'B.'Tarnowski,'A.'J.'Palmateer,'and'J.'H.'Crane,'University'of'Florida,'
Tropical'Research'and'EducaEon'Center,'Homestead'33031P3314''
''
''
Management'of'Bipolaris'fruit'rot'• Currently'there'are'no'fungicides'
labeled'for'use'on'pitahaya'in'California'and'Florida.''
• Cultural'management'includes'limiEng'canopy'wetness'by'irrigaEng'in'the'morning'so'plant'surfaces'can'dry'quickly'throughout'the'day.''
• Maintain'a'weed'free'planEng'and'remove'and'discard'diseased'plants'(i.e.'stems,'fruits,'and'flowers)'promptly'when'symptoms'occur.'
• (From&the&Sarasota&Fruit&and&Nut&Society)&
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Other'Diseases'
• Collar'rot'(Phytophthora&sp)'reported'in'Vietnam'
• Root'rots'caused'by'Fusarium&sp'and'Alternaria&sp.'
• Do'we'have'these'here'and'is'so,'how'bad'are'they?'
Pitahaya'plant'from'the'field''(July,'2013)'
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8/26/14'
11'
Outer'stem'cut'away'
InfecEon'in'the'xylem'and'phloem'
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8/26/14'
12'
Fusarium&conidia'in'pure'culture'(isolated'from'this'same'plant)&
Another'view,'with'mycellium'
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Which'Fusarium?'
• Fusarium'sp.'been'isolated'for'several'years'by'other'plant'pathologists'
• Dr.'Akif'Eskalen'is'the'first'to'idenEfy'to'species'by'analyzing'DNA'
• The'verdict?'
Diseases'Caused'by'Viruses'• Viruses'are'much'smaller'than'bacteria.''• They'enter'the'cells'of'a'plant'and'are'mulEplied'by'the'
host.'Viruses'live'and'mulEply'only'within'living'cells.''• Viral'diseases'are'usually'introduced'by'the'use'of'infected'
plants'or'by'insects.'They'can'be'spread'to'healthy'plants'by'the'feeding'acEvity'of'sucking'insects'such'as'aphids'and'leanoppers,'or'on'the'hands'and'tools'of'maintenance'workers.'
• 'Currently'there'is'no'chemical'control'for'a'virus'once'it'infects'a'plant.'Although'most'viruses'are'specific'to'only'a'few'types'of'plants,'prompt'and'complete'removal'to'prevent'its'potenEal'spread'is'recommended'when'a'virus'is'discovered.'
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Cactus'Virus'X'
• During'a'survey'of'diseases'of'pitaya'in'Taiwan,'some'plants'were'found'with'systemic'mild'mobling'on'the'stems.'
• Transmission'by'sap'or'graXing,'no'insect,'mite''or'nematode'vectors'are'known'
Cactus'Virus'X'
• Long'flexous'rods'
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!First!Report!of!Cactus#virus#X!on!Hylocereus#undatus!
(Cactaceae)!in!Taiwan!February'2001,'Volume'85,'Number'2'
Pages''229.1'P'229.1''''• A'virus'was'mechanically'
transmibed'that'caused'necroEc'local'lesions'on'Chenopodium&amaran4color'and'chloroEc'lesions'on'C.&quinoa.'This'virus'also'caused'necroEc'lesions'with'chloroEc'halos'on'Gomphrena&globosa'and'small'chloroEc'spots'followed'by'systemic'infecEon'in'Celosia&argentea'
• Electron'microscopic'examinaEon'of'negaEvely'stained'extracts'from'diseased'plants'revealed'a'flexuous'rodPshaped'virus'with'a'length'of'480'to'520'nm.'
Control'of'Cactus'Virus'X'
• Once'the'plant'is'infected,'you'can’t'cure'the'plant'
• The'best'control'is'prevenEon'– Buy'diseasePfree'plants'– Propagate'from'seeds'(most'plant'viruses'do'not'pass'into'the'seed)'
– Clean'pruning'tools'with'10%'Chlorox'plus'soap'inPbetween'plants'
– Get'rid'of'prunings…do'not'give'them'away!''Hard!to!do!when!you!have!a!new!industry!'
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Diseases'Caused'by'Nematodes'Cactus'Root'Knot''
Thank&you!&
Gary'Bender'Farm'Advisor'[email protected]'''
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Viruses and Viral Diseases of Cacti and Succulents
Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology
Introduction
• Overview of viruses
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Introduction
• Overview of viruses • Common diseases and symptoms in plants
Introduction
• Overview of viruses • Common diseases and symptoms in plants • Cactus/succulent specific diseases
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Introduction
• Overview of viruses • Common diseases and symptoms in plants • Cactus/succulent specific diseases • Prevention and management
What are Viruses? – Small plant pathogens, 20-2000 nm (1 nm=25 millionth of an inch)
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Types of Plant Viruses
Viruses
– Composed of nucleic acid (DNA or RNA) surrounded by a self made protective coat protein
Tobacco mosaic virus Cucumber mosaic virus
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Viruses – Require host components and living cells to multiply (=replicate)
Viruses – Require host components and living cells to multiply (=replicate) – Can survive on surfaces for various times
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Viruses – Require host components and living cells to multiply (=replicate) – Can survive on surfaces for various times – Enter through wounds or via vectors
insects nematodes fungi HUMANS
Viruses – Require host components and living cells to multiply (=replicate) – Can survive on surfaces for various times – Enter through wounds or via vectors
insects nematodes fungi HUMANS • propagation • tools • any touching
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Viruses
– Over 1,000 species of plant viruses
Viruses
– Over 1,000 species of plant viruses – Each species may have many strains
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Viruses
– Over 1,000 species of plant viruses – Each species may have many strains – Named for first host and symptoms
Viruses
– Over 1,000 species of plant viruses – Each species may have many strains – Named for first host and symptoms • Tobacco mosaic virus (tobamoviruses)
• Tomato spotted wilt virus (tospoviruses)
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Viruses
– Over 1,000 species of plant viruses – Each species may have many strains – Named for first host and symptoms
– Host range may be narrow (few) or wide (many)
• Tobacco mosaic virus (tobamoviruses) • Tomato spotted wilt virus (tospoviruses)
ageratum amaranthus amaryllis anemone aster baby's breath begonia calceolaria calendula calla lily campanula china aster
Host range of Impatiens Necrotic Spot and Tomato Spotted Wilt Tospoviruses-over 1,000 total
Ornamentals chrysanthemum cineraria coleus columbine coreopsis cosmos cyclamen dahlia delphinium exacum forget-me-not gaillardia
geranium gladiolus gloxinia hydrangea impatiens lobelia marigold nasturtium N.G. impatiens peony petunia phlox
poppy primrose ranunculus salvia sinningia snapdragon stock tiger lily verbena zinnia
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broad bean cauliflower celery eggplant lettuce pea pepper potato snap bean spinach tomato
Vegetables
beggar ticks black nightshade morning glory burdock chickweed galinsoga lamb's quarters shepherd's purse sowthistle purslane yellow clover
Host range of Impatiens Necrotic Spot and Tomato Spotted Wilt Tospoviruses-over 1,000 total
Weeds
Viruses
– Mosaic/Mottle Types of Symptoms
Mandevilla Fern Hosta
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Viruses
– Mosaic/Mottle Types of Symptoms
Bean Lettuce Monocots
Viruses
– Ringspots/Line Patterns Types of Symptoms
Nemesia
Tobacco
Ann Chase
Impatiens
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Viruses
– Flower break
Types of Symptoms
Tulip flower breaking virus Angelonia flower break virus
Portulaca
Healthy Infected
– Leaf deformation
Viruses Types of Symptoms
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Viruses
– Fruit deformation Types of Symptoms
Viruses
– Stunting
Types of disease
Citrus tristeza virus
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Viruses Types of Symptoms
• None
Rose Mosaic
• Rose mosaic virus (=Apple mosaic virus) • Prunus necrotic ringspot virus • Arabis mosaic virus
Multiple viruses involved
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Viruses of Cacti and Succulents
Viruses of Cacti and Succulents
• Difficult to work with due to gelatinous tissue
![Page 32: 06 Bender- Pitahaya Diseasesof Cacti and Succulents Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology dmathews@ucr.edu](https://reader033.vdocuments.us/reader033/viewer/2022060301/5f08742d7e708231d42216f9/html5/thumbnails/32.jpg)
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Viruses of Cacti and Succulents
• Difficult to work with due to gelatinous tissue • Distribution of viruses can be uneven in plants
Viruses of Cacti and Succulents
• Difficult to work with due to gelatinous tissue • Distribution of viruses can be uneven in plants • Not a lot of research on these plant hosts
![Page 33: 06 Bender- Pitahaya Diseasesof Cacti and Succulents Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology dmathews@ucr.edu](https://reader033.vdocuments.us/reader033/viewer/2022060301/5f08742d7e708231d42216f9/html5/thumbnails/33.jpg)
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Viruses of Cacti and Succulents
Cactus virus X
Viruses of Cacti and Succulents
Cactus virus X • Infects many species in Cactaceae: Cereus, Saguaro, Opuntia, Zygocactus and Hylocereus
![Page 34: 06 Bender- Pitahaya Diseasesof Cacti and Succulents Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology dmathews@ucr.edu](https://reader033.vdocuments.us/reader033/viewer/2022060301/5f08742d7e708231d42216f9/html5/thumbnails/34.jpg)
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Viruses of Cacti and Succulents
Cactus virus X • Infects many species in Cactaceae: Cereus, Saguaro, Opuntia, Zygocactus and Hylocereus • Found worldwide
Viruses of Cacti and Succulents
Cactus virus X • Infects many species in Cactaceae: Cereus, Saguaro, Opuntia, Zygocactus and Hylocereus • Found worldwide • Symptoms range from none to distorted aereoles, deformed spines, necrosis, mottling
![Page 35: 06 Bender- Pitahaya Diseasesof Cacti and Succulents Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology dmathews@ucr.edu](https://reader033.vdocuments.us/reader033/viewer/2022060301/5f08742d7e708231d42216f9/html5/thumbnails/35.jpg)
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35'
Viruses of Cacti and Succulents
Cactus virus X • Infects many species in Cactaceae: Cereus, Saguaro, Opuntia, Zygocactus and Hylocereus • Found worldwide • Symptoms range from none to distorted aereoles, deformed spines, necrosis, mottling • 3 main strains known to date
Viruses of Cacti and Succulents
Cactus virus X • Infects many species in Cactaceae: Cereus, Saguaro, Opuntia, Zygocactus and Hylocereus • Found worldwide • Symptoms range from none to distorted aereoles, deformed spines, necrosis, mottling • 3 main strains known to date • Transmitted by grafting, mechanical, plant contact
![Page 36: 06 Bender- Pitahaya Diseasesof Cacti and Succulents Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology dmathews@ucr.edu](https://reader033.vdocuments.us/reader033/viewer/2022060301/5f08742d7e708231d42216f9/html5/thumbnails/36.jpg)
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Viruses of Cacti and Succulents Cactus virus X
• Infects many species in Cactaceae: Cereus, Saguaro, Opuntia, Zygocactus and Hylocereus • Found worldwide • Symptoms range from none to distorted aereoles, deformed spines, necrosis, mottling • 3 main strains known to date • Transmitted by grafting, mechanical, plant contact • NOT transmitted by seed; NO known vector
Viruses of Cacti and Succulents Cactus virus X
• Infects many species in Cactaceae: Cereus, Saguaro, Opuntia, Zygocactus and Hylocereus • Found worldwide • Symptoms range from none to distorted aereoles, deformed spines, necrosis, mottling • 3 main strains known to date • Transmitted by grafting, mechanical, plant contact • NOT transmitted by seed; NO known vector • Found in all plant parts including buds, flowers and fruit
![Page 37: 06 Bender- Pitahaya Diseasesof Cacti and Succulents Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology dmathews@ucr.edu](https://reader033.vdocuments.us/reader033/viewer/2022060301/5f08742d7e708231d42216f9/html5/thumbnails/37.jpg)
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Viruses of Cacti and Succulents Cactus virus X
• Infects many species in Cactaceae: Cereus, Saguaro, Opuntia, Zygocactus and Hylocereus • Found worldwide • Symptoms range from none to distorted aereoles, deformed spines, necrosis, mottling • 3 main strains known to date • Transmitted by grafting, mechanical, plant contact • NOT transmitted by seed; NO known vector • Found in all plant parts including buds, flowers and fruit • Relatively high infection levels in most hosts
Cactus virus X on Hylocereus (Dragon fruit)
![Page 38: 06 Bender- Pitahaya Diseasesof Cacti and Succulents Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology dmathews@ucr.edu](https://reader033.vdocuments.us/reader033/viewer/2022060301/5f08742d7e708231d42216f9/html5/thumbnails/38.jpg)
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Cactus virus X on Hylocereus (Dragon fruit)
Chlorosis and Mottling
Cactus virus X on Hylocereus (Dragon fruit)
Twisting of arms
![Page 39: 06 Bender- Pitahaya Diseasesof Cacti and Succulents Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology dmathews@ucr.edu](https://reader033.vdocuments.us/reader033/viewer/2022060301/5f08742d7e708231d42216f9/html5/thumbnails/39.jpg)
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Cactus virus X on Hylocereus (Dragon fruit)
Reddening Speckling
Viruses of Cacti and Succulents
Other Viruses • Zygocactus virus X, Schlumbergera virus X • Opuntia tobamovirus • Cactus 2 virus (carlavirus) • Saguaro cactus virus (carmovirus, sphere, AZ)
![Page 40: 06 Bender- Pitahaya Diseasesof Cacti and Succulents Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology dmathews@ucr.edu](https://reader033.vdocuments.us/reader033/viewer/2022060301/5f08742d7e708231d42216f9/html5/thumbnails/40.jpg)
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Management • No “cure”
Management • No “cure” • Destroy infected stock
![Page 41: 06 Bender- Pitahaya Diseasesof Cacti and Succulents Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology dmathews@ucr.edu](https://reader033.vdocuments.us/reader033/viewer/2022060301/5f08742d7e708231d42216f9/html5/thumbnails/41.jpg)
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Management • No “cure” • Destroy infected stock • Avoid mechanical transmission: handling, tools, pots, remove plant debris
Management • No “cure” • Destroy infected stock • Avoid mechanical transmission: handling, tools, pots, remove plant debris • Decontaminate tools and surfaces with bleach, quaternary ammonium, chlorhexidine, etc (follow labels)
![Page 42: 06 Bender- Pitahaya Diseasesof Cacti and Succulents Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology dmathews@ucr.edu](https://reader033.vdocuments.us/reader033/viewer/2022060301/5f08742d7e708231d42216f9/html5/thumbnails/42.jpg)
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Avoidance/Testing Protocols
• Scout routinely for symptoms
Avoidance/Testing Protocols
• Scout routinely for symptoms • Isolate particularly susceptible varieties
![Page 43: 06 Bender- Pitahaya Diseasesof Cacti and Succulents Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology dmathews@ucr.edu](https://reader033.vdocuments.us/reader033/viewer/2022060301/5f08742d7e708231d42216f9/html5/thumbnails/43.jpg)
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Avoidance/Testing Protocols
• Scout routinely for symptoms • Isolate particularly susceptible varieties • Eliminate hosts outside GH, weeds, landscape plants
Avoidance/Testing Protocols
• Scout routinely for symptoms • Isolate particularly susceptible varieties • Eliminate hosts outside GH, weeds, landscape plants • Regular testing for most common pathogen/host combos
![Page 44: 06 Bender- Pitahaya Diseasesof Cacti and Succulents Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology dmathews@ucr.edu](https://reader033.vdocuments.us/reader033/viewer/2022060301/5f08742d7e708231d42216f9/html5/thumbnails/44.jpg)
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Avoidance/Testing Protocols
• Scout routinely for symptoms • Isolate particularly susceptible varieties • Eliminate hosts outside GH, weeds, landscape plants • Regular testing for most common pathogen/host combos • Collect younger leaves, with symptoms if available
Thank you
![Page 45: 06 Bender- Pitahaya Diseasesof Cacti and Succulents Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology dmathews@ucr.edu](https://reader033.vdocuments.us/reader033/viewer/2022060301/5f08742d7e708231d42216f9/html5/thumbnails/45.jpg)
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Production of Virus Free Ornamental Plants • Virus infected plants undergo meristem shoot tip isolation with or without heat therapy
Removal of apical dome for virus exclusion
![Page 46: 06 Bender- Pitahaya Diseasesof Cacti and Succulents Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology dmathews@ucr.edu](https://reader033.vdocuments.us/reader033/viewer/2022060301/5f08742d7e708231d42216f9/html5/thumbnails/46.jpg)
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Figure 2. Growth progression of isolated verbena shoot tips. Two days post isolation (A, arrow points to tiny shoot tip), 15 days (B), and 30 days (C).
A B C
Figure 2. Growth progression of isolated verbena shoot tips. Two days post isolation (A, arrow points to tiny shoot tip), 15 days (B), and 30 days (C).
AA BB CC
Isolation and Growth of Apical Meristems
2 days 2 weeks 1 month 6 weeks
Virus detection over time from a new “clean” variety • 18 lines of Diascia newly out of selection/clean up
![Page 47: 06 Bender- Pitahaya Diseasesof Cacti and Succulents Deborah Mathews, Ph.D. Cooperative Extension Specialist/Plant Pathologist Department of Plant Pathology and Microbiology dmathews@ucr.edu](https://reader033.vdocuments.us/reader033/viewer/2022060301/5f08742d7e708231d42216f9/html5/thumbnails/47.jpg)
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Virus detection over time from a new “clean” variety • 18 lines of Diascia newly out of selection/clean up • All 18 had tested negative twice by ELISA
Virus detection over time from a new “clean” variety • 18 lines of Diascia newly out of selection/clean up • All 18 had tested negative twice by ELISA • Kept isolated to avoid contamination