dna sequencing lecture
TRANSCRIPT
Some Basics of DNA Sequencing
Let us consider a very simplified example:
Let us say that our “gene” was the three-base
sequence: ATG
Since the beginning of any read is a bit noisy,
we want to start sequencing before the beginning
of our gene and beyond or after the end of our
gene. So, we will include the two bases upstream of
our gene and two bases downstream of the end of
our gene:
TTATGCA
The rest we will do on the board.