dna replication bio10
TRANSCRIPT
DNA Replication, Transcription and Translation
Turning genes into proteins
DNA Replication
DNA replication
– Takes place in the nucleus
– Requires 2 enzymes
Purpose- to make copies of DNA
How is DNA replication related to mitosis?
Complimentary Base Pairing
• The 2 sides of DNA are not exactly the same, they are complimentary.
• If on one side of the DNA strand there is the nucleotide Cytosine, the opposite side will have Guanine, Adenine always pairs with Thymine.
• Remember Chargaff’s rule!
DNA Replication
DNA is replicated through…
1) The unwinding of DNA’s double helix.
2) Separation of strands by helicase
3) Formation of new complementary strands by DNA polymerase.
Step 2- enzyme DNA polymerase adds complimentary base pairs to the original
parent strand.
Step 1- an enzyme, helicase, opens up the original DNA molecule.
helicase
There are now 2 new DNA molecules each with an original parent strand.
Replication Review
• Where does replication take place?
• What are the enzymes involved and what do they do during replication?
• What is a gene?
• Approximately how many genes do you have?
• How is DNA replication related to mitosis?
• Explain complimentary base pairing. Which scientist helped to prove this? (hint: there is a rule named after him)