dmem formulation
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DMEM fomulationTRANSCRIPT
7/16/2019 DMEM Formulation
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Miltenyi Biotec S.L. (Spain)Phone 91 512 12 [email protected]
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Miltenyi Biotec Shanghai OfcePhone [email protected]
Miltenyi Biotec Inc.12740 Earhar t AvenueAuburn CA 95602, USAPhone 800 FOR MACS,530-888 8871Fax 530-888 [email protected]
Miltenyi Biotec GmbHFriedrich-Ebert-Str. 6851429 Bergisch Gladbach,GermanyPhone +49-2204-8306-0Fax [email protected]
Media formulations DMEM DMEM
with stable glutamine
130-091-437 130-091-438
Components g/L g/L
Inorganic saltsCaCl
2. 2H
2O 0.264 0.264
Fe(NO3)
3. 9H
2O 0.001 0.001
KCl 0.4 0.4
MgSO4
. 7H2O 0.2 0.2
NaCl 6.4 6.4
NaHCO3
3.7 3.7
NaH2PO
4.H
2O
0.125 0.125
Other componentsGlucose 4.5 4.5
Phenol red Na 0.0159 0.0159
Sodium Pyruvate 0.11 0.11Amino acidsL-Arginine.HCl 0.084 0.084
L-Cystine 0.048 0.048
L-Alanyl-L-Glutamine -- 0.868
Glycine 0.03 0.03
L-Histidine HCl.H2O 0.042 0.042
L-Isoleucine 0.105 0.105
L-Leucine 0.105 0.105
L-Lysine.HCL 0.146 0.146
L-Methionine 0.030 0.030
L-Phenylalanine 0.066 0.066
L-Serine 0.042 0.042
L-Threonine 0.095 0.095
L-Tryptophan 0.016 0.016
L-Tyrosine 0.072 0.072
L-Valine 0.094 0.094
VitaminsD-Ca Pantothenate 0.004 0.004
Choline Chloride 0.004 0.004
Folic acid 0.004 0.004
myo-Inositol 0.0072 0.0072
Nicotinamide 0.004 0.004
Pyridoxine.HCl 0.004 0.004
Riboavin 0.0004 0.0004
Thiamine.HCl 0.004 0.004
Reference:
1. Dulbecco et al. (1959) Virology 8: 396-397.