dinabandhu andrews college - centrifugationtypes of rotor fixed angle rotors tubes are held at angle...
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CENTRIFUGATIONFor Sem IV
Continuation from last class lecture
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)5(ln)(2
922
upper
bottom
mpp r
r
rt
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2.1 Rate zonal Centrifuagation
• Here the gradient used has maximum density below that of least dense sedimentingparticle.
• Hence the density gradient is reasonably shalow
• The sample particle travel through the steep gradient and form discrete zones depending upon their sedimenting time
• Particles which differ in size but not in density are separated
rrv
mpp
22 )(
9
2
(min)(max) pm
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2.2 Isopycnic centrifugation
Also known as sedimentation equlibrium centrifugation
• Here the gradient used here has maximum density greater than that of most dense sedimenting particle.
rrv
mpp
22 )(
9
2
(max)(max) pm
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