development of industrially applicable techniques...healthcare s(ge health r(yasui kik tion...

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Page 1: Development of industrially applicable techniques...Healthcare s(GE Health R(YASUI KIK tion o-mail m ce, Biotech al Society is system, eriment n and analys lication o rotein Micr

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Page 2: Development of industrially applicable techniques...Healthcare s(GE Health R(YASUI KIK tion o-mail m ce, Biotech al Society is system, eriment n and analys lication o rotein Micr

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Page 3: Development of industrially applicable techniques...Healthcare s(GE Health R(YASUI KIK tion o-mail m ce, Biotech al Society is system, eriment n and analys lication o rotein Micr

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Page 4: Development of industrially applicable techniques...Healthcare s(GE Health R(YASUI KIK tion o-mail m ce, Biotech al Society is system, eriment n and analys lication o rotein Micr

Bioresources and Photochemistry

Name HAMADA Taisuke E-mail [email protected]

Status Professor

Affiliations The Chemical Society of Japan, Japan Society of Coordination Chemistry, The Electrochemical Society of Japan, Japanese Society of Engineering Education

Keywords Photochemistry, Physical chemistry, Coordination chemistry

Technical Support Skills

・ Measurement of photochemical property ・ Supercritical CO2 extraction ・ Photo energy conversion

Research Contents

[Dye sensitized solar cell] Solar energy have attracted much attentions because it is clean, and it is called green energy. Solar cell is device to convert photo-energy to electric energy. We have been studied a solar cell, which is called the dye sensitized solar cell (DSSC).

We have made DSSCs and studied the effect of the dye on the efficiency under irradiation of sunlight (AM 1.5G) from the solar simulator (Fig. 1). In this study, we have evaluated the photochemical and photoelectrical properties of the dye. Therefore, we are able to measure and to evaluate the photochemical property of various compounds in order to clarify a photochemical ability. [Super critical fluid extraction] We have been trying to obtain the useful compound such as poly-methoxy flavonoid (PMF) by means of super critical CO2 extraction method. The merit of this method is that the temperature for the extraction is relatively low and CO2 dose not remain in an extract.

Available Facilities and Equipment

Solar simulator (WACOM)

Supercritical CO2 extraction apparatus (AKICO)

Department of Bioresources Engineering

Okinawa National College of Technology

Page 5: Development of industrially applicable techniques...Healthcare s(GE Health R(YASUI KIK tion o-mail m ce, Biotech al Society is system, eriment n and analys lication o rotein Micr

Searching for Physiological functional substances from subtropical plants grow in Okinawa area. Name Kei Hirayama Ph.D. E-mail [email protected]

Status Professor

Affiliations Society for NeuroscienceJapanese Society for Engineering Education

Keywords Monoaminergic neurons, Alzheimer's disease, Noni (Morinda citrifolia)

Technical Support Skills

・ Animal tissue culture. ・ Neural primary tissue culture. ・ Scientific educations

Research Contents

Available Facilities and Equipment

Confocal fluorescence microscope Ti-U (Nikon)

Digital camera DS-Qi1-U3 NIS-D set (Nikon)

Bio-clean-bench MCV-B131F-PJ (Panasonic)

Tali image base cytometer T10796 (Life technologies)

Field of study • Nerve protective effects on nigrostriatal dopamine (NSDA) neurons by anti-oxidant

substances from subtropical plants.

• Nerve growth factor-like biological effects from plants grow in Okinawa area.

• GTP cyclohydrolase 1 (GTPCH1) transcription within NSDA neurons coupled to membrane

potential and cAMP production.

Research Objective My research interest is in 1. The general area of stimulus transcription coupling within monoaminergic neurons

and has focused on GTPCH1 as a model gene for study. GTPCH1 is the first and rate-limiting enzyme in the synthesis of tetrahydrobiopterin, the essential pteridine cofactor for tyrosine hydroxylase and the production of dopamine by NSDA neurons.

2. Reach out for scientific educations for children and elders.

Department of Bioresuorces Engineering

Okinawa National College of Technology

Page 6: Development of industrially applicable techniques...Healthcare s(GE Health R(YASUI KIK tion o-mail m ce, Biotech al Society is system, eriment n and analys lication o rotein Micr

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Page 7: Development of industrially applicable techniques...Healthcare s(GE Health R(YASUI KIK tion o-mail m ce, Biotech al Society is system, eriment n and analys lication o rotein Micr

Search for biodiversity of marine organisms and application to conservation Name Naoko Isomura E-mail [email protected]

Status Assistant Professor

Affiliations The Zoological Society of Japan, The Ecological Society of Japan, Japanese Coral Reef Society

Keywords Scleractinan corals, Reproduction, Genetic differentiation, Speciation

Technical Support Skills

・ Genotyping ・ Collecting marine organisms by SCUBA ・ Microbial community analysis by NGS

Research Contents In my study, my purposes are to search biodiversity of marine organisms, especially scleractinian corals, and to apply for conservation.

Available Facilities and Equipment

Capillary Sequencer Next Generation Sequencer

Department of Bioresuorces Engineering

Okinawa National College of Technology

Page 8: Development of industrially applicable techniques...Healthcare s(GE Health R(YASUI KIK tion o-mail m ce, Biotech al Society is system, eriment n and analys lication o rotein Micr

Cloning of the gene for plant stress responsiveness Name SANMIYA Kazutsuka E-mail [email protected]

Status Associate professor

Affiliations The Japanese Society of Plant Physiologists The Molecular Biology Society of Japan

Keywords plant stress responsive gene isoprenoid heat shock protein saponin

Technical Support Skills

・ Gene cloning ・ Gene expression analysis ・ Transgenic plant

Research Contents FPPS gene heat shock protein gene UGT gene ● Cloning of the gene for FPPS

For the first time, the gene for chloroplast FPPS was cloned. FPPS is the key enzyme for isoprenoid biosynthesis. Isoprenoids play roles in plant stress response such as protection against sunlight.

● Heat tolerant transgenic tobacco The gene for heat shock protein was used for the transformation (Fig).

● Cloning of the gene for UGT For the first time, the gene for saponin biosynthesis was cloned. Saponin protects the Brassicaceae crops, cabbage, radish, broccoli against pests.

Available Facilities and Equipment

Electrophoresis apparatus Mupid (ADVANCE)

Gene expression analyser AE6933 (ATTO)

Thermal cycler TP450 (TaKaRa)

wild type

tobacco

heat tolerant

transgenic tobacco

Department of Bioresuorces Engineering

Okinawa National College of Technology

Page 9: Development of industrially applicable techniques...Healthcare s(GE Health R(YASUI KIK tion o-mail m ce, Biotech al Society is system, eriment n and analys lication o rotein Micr

Development of Possible Applications for Local Food Resources Name TANAKA Hiroshi E-mail [email protected]

Status Associate Professor

Affiliations Japanese Society for Bioscience, Biotechnology, and Agrochemistry The Japanese Society for Food Science and Technology

Keywords Local resources, Lactic acid bacteria, Food processing, Marketing

Technical Support Skills

・ Preparation of foods and/or ingredients ・ Nutrient analysis and functionality evaluation ・ Functionality of lactic acid bacteria ・ Development of marketing plan for new products

Research Contents

Available Facilities and Equipment

Lactic acid bacteria culture system

UPLC (Waters)

Cell culture system

Department of Bioresuorces Engineering

Okinawa National College of Technology

Page 10: Development of industrially applicable techniques...Healthcare s(GE Health R(YASUI KIK tion o-mail m ce, Biotech al Society is system, eriment n and analys lication o rotein Micr

Environmental impact assessment based on environmental responses of organisms

Name Akira Iguchi E-mail iguchi.a��������� ��� � ��

Status Assistant professor

Affiliations

Ecological Society of Japan, Japan Society on Water Environment, Japanese Association of Benthology, Society of Evolutionary Studies Japan, Tthe Biological Society of Okinawa, Japanese Coral Reef Society

Keywords Environmental response, Conservation, Environmental

impact assessment, Adaptation, Genome

Technical Support Skills Genetic analysis, Statistical analysis

Research

Contents

Establishment of environmental impact assessment using sessile organisms

I am aiming to conserve ecological systems which have been negatively impacted by recent environmental changes (global warming, ocean acidification, air pollution, eutrophication, etc) focusing on environmental responses of sessile organisms (e.g. plants, marine benthos) , and establish environmental impact assessment and management for bioresources.

Available Facilities and Equipment

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Page 11: Development of industrially applicable techniques...Healthcare s(GE Health R(YASUI KIK tion o-mail m ce, Biotech al Society is system, eriment n and analys lication o rotein Micr

Research Contents

High efficiency extraction and food processing using the shock wave Name Takemoto Ayumi E-mail [email protected]

Status Assistant Professor

Affiliations The Japan Society of High Pressure Science and Technology, The Japan Society of Cookery Science, The Materials Research Society of Japan

Keywords Shockwave processing, Milling, Extraction, Non-thermal sterilization

Technical Support Skills

・ Shock wave processing to biosystem ・ Non-thermal milling, Efficient extraction

Available Facilities and Equipment

Shock wave processing

Department of Bioresuorces Engineering

Okinawa National College of Technology