Develop a Bandwidth Detection System to Monitor Metabolic Activity of Single Cells

Jerry Wilmink and Jonathon WellsAdvisor: Franz BaudenbacherBME 273, Group 9

Project Inception?

BioMEMS Device Funded by DARPA Cell based sensing

technology Monitor Cell

Metabolism Subnanoliter volumes

BioMEMS

Fabricated on Silicon Chip Peristaltic Pumps Fluidics Layer

Interdigitated Array electrodes

BioMEMS

Combine electrodes with flourescence probesQuantify and Classify the impact of various toxins on cell physiologyCBW Agents to develop signatures

Expected Expected Acidification Acidification Rate of a Single Rate of a Single Cell in a 0.1 nL Cell in a 0.1 nL Volume Volume

0.0 0.2 0.4 0.6 0.8 1.05.6

6.0

6.4

6.8

7.2

pHTime [s]

Project Overview

Step 1:Design the hardware for a 256 channel data acquisition system to read out the Hamamatsu photodiode array

Project Overview

Step 2:Design Labview program to control the data acquisition system and provide control signals for the valves driving the bioMEMS device

Project Overview

Step 3:Design Hardware to interface the photodiode array with an inverted microscope and the bioMEMS devices

Project Overview

Step 4:Devise a calibration protocol for the photodiode arraybandwidth of dye

Project Overview

Step 5:Record transmembrane potential of single cardiac myocytes with flourescent probes and record from electrochemical sensors

Progress

Research Darpa ProposalJournals, Internet etc

Began Step 1:Install LabviewInstall NI data acquisition boardSoder individual channel to corresponding photo diodeApply current to bioMEM to make sure it worked

Future Plans

Begin Step 2:Design Labview programThat enables specific channel identification That is read in from specific photodiodesWork with potentiostat readout and manipulate and quantify results