determining infectiousness of tb patients · content • determining tb infectiousness: –clinical...
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Determining Infectiousness
of TB Patients
Edward A. Nardell, MD
Brigham & Women’s Hospital, Division of Global Health Equity
Professor of Medicine, Global Health/Social Medicine,
Harvard Medical School
&
Professor of Environmental Health
Harvard School of Public Health
Partners In Health, Boston, USA
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TB Rates over the Last Century
Cape Town, New York, and London
Driven by transmission
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Content• Determining TB Infectiousness:
– Clinical – cough, cavitary CXR, smear pos.
• Mtb strain infectiousness, environment.
– Public health – contact investigation.
• Concentric circle approach
– Quantitative air sampling
• Cannot culture Mtb from ward air
– Can from artificial aerosols of BCG, e.g.
• Human to guinea pig transmission
• CASS – cough aerosol sampling
• Molecular detection of Mtb DNA
• Viable particle counting.
– CO2 monitoring – rebreathed air fraction
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Propagation of Mycobacterium tuberculosis
Aerobiology
Pathogenesis Infection &
Re-infection
disease
Host
resistance
Source
strength
Organism
number
viability
phenotype
Aerosolization Lung impaction
Immunization
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Wells’ Air Centrifuge, 1931
“On Airborne Infection, Study II. Droplets and Droplet Nuclei”
W. F. Wells*. Am J Hygiene, 1934:20. 611-18.
*Instructor, Sanitary Service, HSPH
Wells developed his air centrifuge to sample bacteria from air in a New England textile mill. Richard Riley was his student.
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Wells/Riley Experimental TB Ward• Riley RL, Mills C, Nyka W.
Aerial dissemination of tuberculosis – a two year study of contagion on a tuberculosis ward. Am J Hyg 1959; 70:185-196.
(reprinted as “classic” Am J Epidemiol 1995; 142:3-14)
• Riley RL. What nobody needs to know about airborne infection. (How It Really Happened) AJRCCM 2001; 163:7-8.
Quantitative air sampling for TB
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Wells/Riley Ward – Results (Exp 2)
• 2.6 GPs infected per month
– strict criteria
• Relative infectivity of patients*:
– Susceptible TB• 61 Untreated (29 GPs) 100
• 29 Treated (1 GP) 2
– Drug-resistant TB• 6 Untreated (14 GPs) 28
• 11 Treated (6 GPs) 5
*all smear positive patients, relative to the amount of time on the ward
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Human to GP results
• Mtb is airborne
• Highly variable infectiousness
– Mtb variability (Beijing, etc)
• Drug resistance – fitness cost?
– Source strength highly variable
• q = 1.25 vs 13 vs 60 vs 250 qph
• Effective treatment stops transmission
quickly
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The Airborne Infections Research (AIR) Facility
Witbank, Mpumalanga Province, SA
Dedicated 2005,
Rededicated 2015
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Airborne Transmission - Interventions
Aerobiology
Environmental stresses:
Temperature and humidity
radiation
Pathogenesis infectiondisease
Host
resistance
Source
strength
Organism
Treatment**
drug resistance
number
viabilityVirulence
Take off Landing
IsolationAdmin.
Controls
“F-A-S-T”
Dilution (ventilation)
(Filtration)
UVGI**
ImmunizationResp Protection
Masks on
patients
Treatment of latent infection
Dose
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AIR, Experimental Plan
A B
Odd days Even days
3 patient rooms
Plus common areas
Intervention on/off on alternative days
Guinea Pig Air Sampling
Pt. TB
RFLP
Guinea Pig
TB RFLP
UVGI or
other
intervention
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Ventilation ducts in patient rooms
Paddle Fans Assure Good Air Mixing
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ResultsUV1 Intervention Control
TST-1 0 1TST-2 0 3TST-3 0 5TST-4 0 0TOTAL 0 9
UV2 TST1 3 17 TST-2 12 30TST3 0 1TOTAL 15 48
*p<0.0005
Combined hazard ratio 4.9 (CI.95: 2.8, 8.6) or about 80% effective.
Note: Equivalent of adding 24 ACH to the AIR facility wards
AJRCCM – available on line
Haza
rd o
f beco
min
g infe
cted
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Riley study 30 W (nominal)
Per 200 sq ft floor area
Does not take lamp or
fixture efficiency
into account
NIOSH study
(primary)
30 – 50 µW/cm2 avg. UV
fluency rate (measured in
a horizontal plane at the
level of the lamp)
Hard to predict in advance
No standard measurement
method
NIOSH study 6.3 W total UVGI lamp
wattage per cubic meter
upper room volume
Does not take fixture
efficiency into account
AIR Facility
study (primary)
6 µW/cm2 avg. UV fluency
rate (for the entire room)
Requires gonioradiometry
of fixtures for input into
Visual-UV CAD program
(incorporates ray length)
AIR Facility
study
(practical)
15-20 mW total fixture
UV output/m3 room
volume
(or approx 20 mW/m3)
Requires Total Fixture UV
Output measurement
(supplied by manufacturer)
Must adjust for avg ray
length in a given room
New UVGI Dosing Criteria:
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How Effective are Surgical Masks on Patients?
For health care workersFor patients
Respirators
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Guinea
Pig
Gro
up
TST 0 TST 1 TST 2 TST 3 TST 4 Total
Intervent
ion
0 1 10 20 5 36
Control 0 4 15 39 11 69
How Effective Are Surgical Masks on Patients?
Approx 53% Effective
Dharmadhikari AS, et. al.
Am J Respir Crit Care Med.
2012 May 15;185(10):1104-9.
NIOSH funded
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What is Infection?
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Hematogenous spread
Latent TB Infection
Stead’s “Unitary Theory” of tuberculosis pathogenesis
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Laboratory exposure 15 – 30 cfu
All animals clearly infected, all die
Palanisamy GS. et.al. Tuberculosis (2008).
Our GPs were sacrificed at
approximately 280 days
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Observations from AIR
Pilot Study1. 53 guinea pigs reverted their skin test back to 0 mm.
2. No guinea pigs that reverted had signs of TB or
pathology
0
5
10
15
20
0 4 8 12 18
GP 8
0
5
10
15
20
0 4 8 12 18
GP 33
Timing of TST (weeks) Timing of TST
(weeks)
TS
T s
ize
in
mm
TS
T s
ize
in
mm
0
20
40
60
80
100
TST1 2 3 4 5
33 guinea pigs reverted and had another episode of TST reactivity
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Nardell & Wallis, AJRCCM
2006:174
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Exposure – no infection (91, 25%)
Exposure – infection (271, 75%)
Exposure – infection – reversion (53,15%)
Exposure – infection – reversion - reinfection (33, 9%)
Exposure – infection – (reinfection) – disease (54,15%)
Tuberculosis, 2011. 91(4):
p. 329-38
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0
5
10
15
20
0 4 8 12 18
GP 338
0
5
10
15
20
0 4 8 12 18
GP 251
GP Evidence of Reinfection
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Don Smith Alterative Pathway
to Cavitary TB
Smith DW, et al. Rev Infect Dis 11: S385-S393 (1989)
Stead Theory
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Hospitals as Drug Resistant TB Factories
Drug Susceptible TBor
No TB at all
Drug Resistant or
DS TB
Health care workers and patients at great risk of infection and deathHIV co-infected at greatest risk
Half of the 500,000 new MDR cases per year are transmitted.
Hospitalized drug susceptible TB patients in Tomsk, Siberia
are more that 6 times more likely to develop MDR-TB
Glemanova, et al, Bull WHO, 2007; 85:703-711.
. Gila Kaplan, BMGF, “its all about transmission”
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Hospitals as Causes of Human Suffering
Referring to the Hotel-Dieu in Paris which had a mortality rate of 1 in 4 patients
“A fragment of space closed on itself, a place of internment of men and disease, its ceremonious but inept architecture multiplying the ills of its interior without preventing their outward diffusion, the hospital is more of a centre of death (foyer de mort) for the cities where it is sited, than a therapeutic agent for the population as a whole”
Ref: Medicine and Magnificence – British Hospital and Asylum Architecture, 1660 – 1815, by Christine Stevenson, Yale University Press, 2000, page 155
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Florence Nightingale 1820-1910
“Notes on Hospital Design 1859”
Key role of nurses in airborne
Infection control research
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Building Design and Engineering for
Airborne Infection Control (2008-2016)
One week courses this summer in Lima, Peru; and Pretoria, South Africa
Two-week course Harvard Course, Boston, Aug 1- 16, 2016
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Indus Hospital, MDR Clinic
Karachi, Pakistan
Butaro District Hospital,
Rwanda (PIH, MASS Design Group)
Alert Hospital, MDR Clinic
Addis Ababa, Ethiopia
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How Do We Know These Building
Designs Work – or Don’t?
• Hypothesis: Average personal shared air
experience by workers in different hospitals
reflects building design and use.
• Personal ambient CO2 monitoring represents
the balance between production (40,000
ppm) by occupants, and reduction by
ventilation (400 ppm outside)
– An index of shared air – indicator of risk of TB
infection if there is an infectious source
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Ethiopia: St. Peter’s New Building
Very large interior space – glass!!
No ventilation
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How do we know these designs work?
Current Assessment Tools
• Smoke tubes
• Flow meters
– Vanometers
• Tracer gases
– CO2 decay
Generally reflect a
moment in time
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Concept
• Avg personal ambient CO2 over 4 – 8 hrs
reflects avg rebreathed air experience.
– An index of airborne risk given a source of
infection
• Reflects building ventilation AND its use,
especially occupancy.
• Compare nurses doing the same job in two
different hospital settings
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Risk of indoor airborne infection transmission estimated from carbon dioxide
concentration. Rudnick SN1, Milton DK. Indoor Air. 2003 Sep;13(3):237-45.
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Other Hospital Evaluations
Planned in South Africa
• Tygerberg Hospital, Stellenbosch
• Kimberly Hospital
• New London Hospital
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New Transmission Issues
• Global warming – need for AC
– Split systems increasingly common – no ventilation
• Windows closed in ambulatory center, Mumbai
• MDR/XDR surgery – how to do it safely
– Backlog of cases in Peru – staff fearful
– Major concern of Lion Hospital, Ethiopia
– ORs normally positive pressure
– Isolation rooms normally negative pressure
• Role for GUV and PAPRs
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Cough Aerosol SamplingKevin Fennelly, MD
TB Patient
Each patient performs 2 cough sampling
sessions over 2 successive days
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Instantaneous Viable Particle
Detection SystemA new potential tool to evaluating air disinfection
• Particle counter – sorts
viable and non-viable
• Not Mtb specific –
environmental organisms in
facilities vs. Mtb under
aerosol chamber conditions
• Instantaneous read out
• Can be used on occupied
ward
• Detects NADH and riboflavin
as viability biomarkers
Sample
Airflow
Laser Beam
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Conclusions:• Determining TB Infectiousness:
– Clinical – cough, cavitary CXR, smear pos.
• Mtb strain infectiousness, environment.
– Public health – contact investigation.
• Concentric circle approach
– Quantitative air sampling
• Cannot culture Mtb from ward air
– Can from artificial aerosols of BCG, e.g.
• Human to guinea pig transmission
• CASS – cough aerosol sampling
• Molecular detection of Mtb DNA
• Viable particle counting.
– CO2 monitoring – rebreathed air fraction