design and applications of luminescent logic systems
TRANSCRIPT
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Design and Applications of Luminescent Logic Systems
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generalization
integration
numeracy
games
reconfiguring
object identification
improved sensing
MolecularLogic Gates
Nimal Gunaratne, Colin McCoyNature 1993, 364, 42
George Boole1849-64, Cork
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Kim, Yoon,Choi, Zhang
Lehn
Bouas-Laurent, Desvergne,Bassani, McClenaghan Balzani, Credi,
Venturi, Gentili,
Bharadwaj, Khan,
Samanta
Adleman,Ghadiri,
de Silva,Yurke
Pina, Pischel
Garcia-
Brown
Raymo, Zhu,
Akkaya
Ji
Gunnlaugsson,MacDonaill
Birge, Stojanovic,Seeman,Avouris,Katz, Privman,Sokolov, Wang
Langford,Stefanovic,
Wasielewski,
Levine, Speiser,Shanzer,Willner,Shabat,Ashkenasy,Lotan,Van der Boom,Rabani, Shapiro, Eichen
Konermann Remacle
Reif
Diederich, Constable, Schluter
de SilvaWettig
Inestrosa,Jin
Schneider, Rurack,
Uchiyama, Aida,
Leigh,Callan
James, Stoddart, Parker, Beer, Chin, Grigg,Williams, Tucker, Zauner, Fallis, Aldridge
Szacilowski,
Chiu
Gust, Moore
Steer, Yeow,
Iwai, Okamoto,Sugimoto, Hirai,
Redmond,
Miranda,
Kimura,
Miyashita
Tian, Wang, Jin,
Lu, Ma
Lerner,Barbas,
Wu
Toma
Conrad, Liu, SmithBazan
Andreasson, Sun
Liu
Tanaka, Fujita,
Breaker,
Das
Kumar,
Lu
Zhang, Zhu, Yan, Li, Fu,
Huang, Zhang, Yu,
Mihara, Nojima,
Tomizaki, Matsui,
Pischel
Fujimoto, Akashi,
Qian, He
Yang
Benenson, Walt
Stoddart, Heath, Smolke
Vasquez-Lopez
Yuan, Fang,Voegtle, Schmittel,
Shi, Tong,
Hamilton
Doorn
Wang, Xing,
Kinbara
Miyashita, Hamachi
Samoc
Callan,
Perez-
Espana,
CoveraKolpashnikov,
Ihmels, Theato
Wang
Bhalla,
Upadhyay
Wu, Shi
Singh
Pallavicini, Fabbrizzi,
Kubo
Campagna, Quici
Evans
Johnson
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D’Souza
JiaSuzuki, Araki
Budyka
Alexander, Jones, Steed
Nandi
Jang
Alfonta
Humphrey
Magri
Hill, Ariga
Stoddart
Zink,
deSouter-Lecomte
Pasparakis
PandeySiri
Mayer, Nau, Schalley,
Dwyer
Sessler, Tour
Jiang
Ha, Lee, Kim, Oh
Plaxco
Seeberger, Schiller
Kandaswamy
Jiang
Zhang
Wang, Qu,Dong, Ren,
Zhu
Yamada, Ogawa,
Kong,
Ruiz-Molina
Huang
Zhang, Ye,
Pita
Ajayaghosh
Maeda
Tuntulani
Shvarev
HanleyDarwish,
Stokke
Wang, Jiang, Hu
Cheng
Leung
Flood
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011
100
OUTOUTIN
NOTYES
IN IN
OUT OUT
111
001
010
000
OUTIN2IN1
AND
IN1 IN2
OUT
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Luminescent molecular switchable systems can gather and process information
Optical response
Chemical stimulus level
Analog region
Digital 1
Digital 0
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Molecular Computation in a Small Nanospace
Seiichi Uchiyama, Gareth McClean,Kaoru Iwai (Nara Women’s University)
J. Am. Chem. Soc. 2005, 127, 8920
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3 nm
Soap micellewith ion cloud
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O
OO
O
O
N
fluorophorespacer spacerreceptor2
anchor
Na
receptor1
H
fluorophorespacer spacerreceptor2
anchor
Na+
receptor 1
H+
OSO3 N
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400 5000
400 500Wavelength (nm)
HH+
Na+
HH+
Na+
none
and
IF
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Combining Several Diagnostic Tests and Data Processing
in a “Lab-on-a-Molecule”
David Magri, Gareth McClean, Gareth Brown
J. Am. Chem. Soc. 2006, 128, 4950
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Full Blood CountDate : 20.08.2005
Description Result Units Reference Ranges RemarksHaemoglobin 8.70* g/dl 12.00 17.50 LowRBC 2.34* x 10x6/mm3 3.50 6.00 LowPCV 26.70* % 40.00 54.00 LowMCV 114.10* fl 70.00 96.00 HighMCH 37.20 pg 27.00 32.00 HighMCHC 32.60 g/dl 30.00 ….35.00 …NormalPLATELETS 140,000.00 mm3 150,000.00 450,000.00 LowWBC 8,200.00 mm3 4,000.00 11,000.00 NormalNEUTROPHILS48.00 % 40.00 75.00 …normalLYMPHOCYTES48.00 % 10.00 45.00 HighEOSINOPHILS 2.0 % 1.00 6.00 NormalMONOCYTES 2.00 % < 10.00 Normal
Performed by Sysmex/Hycell Automated Haematology Analyzers
Logical combination of ‘high’ and ‘low’
parameters identifies disease
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Spacer ReceptorReceptor Spacer SpacerFluorophore Receptor
O
OO
O
O
N
N CO2
CO2
-
-
HH+Na+
Zn2+
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Wavelength / nm
400 440 480 520
+
Na+ , H+ , Zn 2+
Na+ , H +
Na+ , Zn 2+
Na +
H+ , Zn 2+
Zn 2+
H
No inputs
0
IF
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1 (high, 0.020)1 (high)1 (high)1 (high)
0 (low, 0.006)1 (high)0 (low)1 (high)
0 (low, 0.007)0 (low)1 (high)1 (high)
0 (low, 0.006)0 (low)0 (low)1 (high)
0 (low, 0.003)1 (high)1 (high)0 (low)
0 (low, 0.002)1 (high)0 (low)0 (low)
0 (low, 0.001)0 (low)1 (high)0 (low)
0 (low, 0.001)0 (low)0 (low)0 (low)
Output Fluorn F
Input3
Zn2+
Input2
H+
Input1
Na+
3-Input AND Logic Truth Table
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Molecular Computational Identification (MCID)
of Small Objects in Populations
Sheenagh Weir, Bernie McKinney Dave Pears, Mark James (Avecia)
Nature Mater. 2006, 5, 787
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Radiofrequency Identification (RFID): The semiconductor technology approach to identifying
each object (Goods, People) in an entire population
1 mm x 1 mm RFID chip (Hitachi)
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EXCITATION WAVELENGTH: 368 nm
NNH
O
500Wavelength (nm)
Flu
ore
scen
ce
Inte
nsi
ty
385
100 Increasing pH
422
401
446
Prefabricated YES logic gate fixed to polymer bead (0.1 mm)
0
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Prefabricated logic gates fixed to polymer beads
NNH
O
YES O
NH
PASS 1NO
NH
NOT
0
3 5 7 9 11pH
100
IF
1
YES
PASS 1
NOT
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Arraying gates with identical fluorophores
and different logic type
2.5 % loading 2.5 % loading
Wavelength (nm)385 500
100
Increasing pH
422
401 446
O
NH
NNH
O
0
IF
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PASS 1 YES PASS 1 + YES
Input(H+)
Output (Fluorescence)
Output (Fluorescence)
Total Output(Fluorescence)
0 1 0 1
1 1 1 2
pKa : 4.4
0
20
40
60
80
100
2.5 4.5 6.5 8.5 10.5
pH
Em
iss
ion
(at
422
nm
)
OUT( Blue fluorescence)IN
(H+)OUT( Blue fluorescence)
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Multi-valued logic is available for molecular computational identification c.f. binary logic needed in semiconductor computing
Combinations of:Many (>5) excitation colours
Many (>5) emission colours
Many (>5) types (YES, NOT, PASS 1, AND,…)
Many (>5) chemical inputs (H+, Na+,…)
Many (>2) thresholds (pH 7, pH 4,…)
Many (>5) combinations (YES + PASS 1, YES + 2 PASS 1,…)
Conservatively, millions of m objects can be encoded!
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acid
alkali
A
A
B
B
C
C
D
D
E
E
F
F
G
G
I
I
J
J
A; PASS 1 B; NOT C; PASS 1 D; PASS 1 + YES (1:1) E; YES F; NOT G; PASS 1I; YES J; PASS 0
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“Plug-and-Play” Logic via Self-Assembly
Vinny Vance, Catherine Dobbin, Boontana Wannalerse
Chem. Commun. 2009, 1386
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_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
__ _
__
__
R
L
S RL
Self-Assembled YES Logic:
Covalently bound YES Logic:
Assembler: Non-ionic Detergent Micelle
N
N
2
N
N
n-C9H19
n-C9H19
RuII
OOH
10
O-
Long-lived (100 ns) lumophore
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O-
pKa = 9.9
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_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
__ _
__
__
R 1
L
R2
SS R1L R2
Self-Assembled AND Logic:
Covalently bound AND Logic:
O-
N
CO2 CO2
On-C8H17
- -
pKa = 9.9 pKa = 5.8 Log Ca2+ = 1.5
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11; 10-8 M H+ and 0.2 M Ca2+ 01; 10-12 M H+ and 0.2 M Ca2+
10; 10-8 M H+ only
00; 10-12 M H+ only
O-
N
CO2 CO2
On-C8H17
- -
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_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
_
__ _
__
__
R
L
S RL
Self-Assembled OR Logic:
Covalently bound OR Logic:
Non-selective Receptor
N
CO2 CO2
On-C8H17
- -
binds H+ or Ca2+
pKa = 5.8 Log Ca = 1.5
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11; 10-4 M H+ and 0.2 M Ca2+ 10; 10-4 M H+ only
01; 10-8 M H+ and 0.2 M Ca2+
00; 10-8 M H+ only
N
CO2 CO2
On-C8H17
- -
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Logic Gates withTemperature Input
Seiichi Uchiyama, Narumi KawaiKaoru Iwai (Nara Women’s University)
J. Am. Chem. Soc. 2004, 126, 3032
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Temperature-sensitive co-polymer
O
O
N
SO2NMe2
NO
N
Co-polymerizable polarity sensor(Emission weakens in water)
NH
O
NMe2
NH
O
and
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20 30 40
Temperature / C
0
IF
10
pH 7
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●
20 40 60
Temperature / C
0 80
pH 9
pH 8
pH 7
pH 5
0
IF
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2-Input INHIBIT Logic Truth Table
0 (low; 1)1 (high; 10-5 M)1 (high; 35 C)
1 (high; 10)0 (low; 10-9 M)1 (high; 35 C)
0 (low; 1)1 (high; 10-5 M)0 (low; 10 C)
0 (low; 1)0 (low; 10-9 M)0 (low; 10 C)
Output
Fluorescence
Input2
H+
Input1
Temperature
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ProvidenceProvidence
LuckLuck
SerendipitySerendipity
EPSRCEPSRC
DENIDENI
EUROPEAN COMMISSIONEUROPEAN COMMISSION
JAPAN SOCIETY FOR JAPAN SOCIETY FOR THE PROMOTION OF SCIENCETHE PROMOTION OF SCIENCE