1

1. Faculty profile

Sr. No.

Faculty Designation Age

(years) Educational

qualifications

Teaching experience

(years)

Appointment date

No. of publicati

ons

1. R M Kothari Professor &

Principal 69 Ph.D. DBM 18 01/06/09 223

2. R K

Bhadekar

Asst.

Professor 41 Ph.D. 08 10/09/04 9

3. N K Bipinraj Lecturer 33 Ph.D. 04 01/08/05 4

4. D P Samarth Teaching

Assistant 26

M.Sc.

(Microbiology) 01 01/08/09 -

2. Infrastructure for teaching and learning

Laboratory facilities

Floor Laboratory particulars Dimensions Area (Sq. ft.)

Capacity

First

Microbial biotechnology (UG) 22.21 X 38.61 857.5 24

Microbial biotechnology (PG) 22.21 X 38.61 857.5 18

Research laboratory 22.21 X 28.77 638.98 08

Washing room 18.37 X 9.41 172.99 -

Incubation room 13.77 X 8.80 122.06 -

2

December 22nd 2008

Dr. M. K. Bhan,

Secretary, DBT

Government of India

visited the department

3

Practicals conducted :

Disinfection and sterilization

Pure culture techniques Microscopy Media preparation Isolation of

microorganisms Characterization Staining techniques Bioassays

Microbial biotechnology

(UG) Laboratory

4

Practicals conducted :

DNA isolation from pro and eukaryotes

RNA isolation Agarose gel

electrophoresis Restriction digestion,

ligation

Cloning PCR Southern

hybridization Primer designing

Microbial biotechnology

(PG) laboratory

5

Research laboratory

Projects underway:

Microbial production of

omega-3-fatty acid (In

collaboration with

IRSHA, BVDU) funded

by DBT, Govt. of India

Other projects are related

to:

Marine biotechnology

Probiotics

Antimicrobial

compounds from

medicinal plants

6

2009: Fermentation

and

scale up facility

established

7

3. Instrumental facilities

Major Equipment Make Cost (Rs.) Date of purchase

PCR Eppendorf 620801 15/09/04 Centrifuge Plastocrapt 488796 06/09/05 Gel documentation B-Genei 472388 23/08/06 Laminar air flow unit-03 Micro filt -02

Envair-01 239759 23/11/05,

09/09/08 Cooling Microfuge Remi 124763 14/11/06 Electrophoresis power supply-04

B-Genei 117896 22/08/05, 10/04/06

Orbital shaking incubator Remi 103140 05/10/05 Electronic balance-01 Mettler 84375 12/03/08 Microscope binocular -02 Olympus CH20 80550 24/08/05 Compound Microscopes – 5 Magnus 80265 08/09/09 Trans-illuminator-02 B-Genei 78570 19/07/06 Deep freeze (-400C) -01 Remi 66402 23/08/08 Ligation bath-01 B-Genei 48094 01/10/05 Hot air oven-02 Bio-Technics 44404 27/08/05 Sony VPL EX-7 LCD projector (Class room) -02

Space office system Pvt. Ltd.

44000 28/08/09

Incubators -03 Bio-Technics 42306 27/08/05 Refrigerator -02 LG 37400 03/09/04 Rota evaporator -01 Superfit 36110 15/05/07 Distillation unit (Glass-01) Local, 28245 05/10/05 Autoclave-01 Lab hosp 26530 04/01/05 Autoclave -01 From Hotel

Management 0 NA

Water bath -02 Bio-Technics 20429 14/09/05 Colorimeter -02 Equiptronics

Systronics 19850 16/12/08

04/09/04 Balance-02 Tapson, Master 19800 27/08/05 Water bath shaker-01 Neolab 17865 05/10/05 Digital pH meter-01 Coslab 15946 08/09/09 Total 2958684

Minor Micro-oven -01 Lloyd 8505 08/09/09 Digital pH meter-01 Global 8415 27/08/05 Vortex mixer-03 Neolab -01,

Remi -02 6589 23/09/05

24/02/07 Fixa-pipette set (5 µl , 25 µl, 50 µl, 100 µl, 500 µl & 1000 µl)

Fixa-pipette & Bioera

6048 08/09/09

Variable pipette (10µl-02, 20µl-01, 100µl-02, 200µl-01 &

Pipette 23275 22/08/05

8

1000µl-01) Magnetic stirrer Spinit 4890 09/09/09 Digital colony counter-01 Elite 4275 24/02/07 Bench top cooler-02 (B-Genei) 3527 19/07/06 Magnetic stirrer-01 Neolab 2848 23/09/05 Mixer Phillips 2457 08/09/09 Hot plate-01 Bio-Technics 1197 27/08/05 Stopwatch-02 Racer 900 08/09/09 Total 72926

Project equipments Distillation unit (Steel-01) Local

Lab Enterprise 7834 18/02/09

Table top Centrifuge-01 Remi 16052 10/04/08 Colorimeter -01 Equiptronics 9450 16/12/08 Ultrasonicator Trans-o- sonic 90208 30/03/09 Lyophilizer Biotechnologies 295481 30/03/09 Fermentor Napro 1172712 31/03/09 Total 1591737

Grand total 4623347

9

4. Achivements

Reviews submitted by T. Y. B. Sc. students:

This activity was initiated for the T. Y. B.Sc. students to impart them

skills of understanding the scientific subjects, comprehending relevant

information in it and analytically summarizing the same in the form of a

brief review.

Sr.no. Title

Author

2005 - 2006 1 Flavonoids Kaveri Sidhu 2 Bacterial pigments Mangesh S. Sawant 3 Malaria, its cure and prevention Rajiv Choudhary 4 Single cell protein Sarika Mane

5 Dengue virus : genome sequencing , prevention & cure

Abhinav Mishra

6 Signal peptides in prokaryotes : Literature review

Namrata Ghaytidak

7 Carotenoids Tanya Soni

8 Geneti variance exhibited in the Indian population :its significance in pharmacogenomics

Kartik Rajan

9 Trichoderma as a bio Control agent Manish Kumar Pandey 10 Proteases Aduja Naik 11 Recombinant Interferon Veenita Shukla 12 Bio preservatives Manpreet Grewal 13 Hepatitis-C Jayant Pawar 14 DNA-fingerprinting Swapanaja Patil 15 Literatiure review osteoporosis Shewta Padhye

2006-2007 1 Recombinant Vaccines Deepali Singh 2 Detailed study of AIDS Amol Laddha 3 Biofertilizers Pradnya Nikam 4 Alternative therapy Jyoti Dipty 5 Bio Fertilizers & Bio Pestisides Anup Shah

6 Synthesis of commercial recombinant microbes

Sushmita Chawdhury

2007-2008

1 Food and drug administration Leena Hedge 2 Bio Fertilizer-Azotobacter Bhagyashree Rajapure

3 The study of gourds with special reference to ashgourds, bottle gourds, bitter gourds, ridge gourds, snake gourds.

Vandita D’souza

10

4 Value of waste from brightly coloured vegetables

Sonam S. Dalimbakar

5 Phytase : transforming a curse into a boon Rohan Kapoor 6 Flavonoids : Grape skin Priyanka Tiwari

7 Biodegradation of polytheyene by microorganisms

Ankita Singh

8 Diabetes: Its herbal remedies Sarita Kumari 9 Acacia concinna Neha Jaiswal 10 Xenobiotics: Recalcitrant compounds Bahul Gidde 11 Wine making Sagar Mahind 12 Wine & human health Suman Ranjan Jha 13 Rhizobium as a biofertilizer Twisha Singh

2008-2009 1 Current status of hepatitis C vaccine Debanjana Dutta 2 Steroids and their effect on human health Deepshikha Saraswat 3 Probiotics: Immunomodulatory effects Arti Kateria 4 Probiotics Amar Patil 5 Biological databases Neel Shah 6 Recovery of PUFA Shweta Arora 7 Types of pollution in Antartica region Tolnur Malkarjappe 8 Herbs as skin treatment Monica Athwani

9 Biodegradation of polyethylene by microorganisms

Parinita Jhawar

2009-2010

1 Trichoderma viride: Its cultivation and Applications

Aanchal Katoch

2 Pathogens: Sources, Incidents, Fatalities and Remedies

Ankita Narang

3 Transformation of Penicillin in to APA - Significance

Vijaya Lakshmi

M. Sc. projects completed

Sr. no. Title of project Name of student 2006 - 2007

1. Bio-diesel Deshmukh Siddharth 2. A review on Spirulina platensis Kadam Deepa 3. Citric acid production by Aspergillus

niger Sagolsem Bidhanchandra

4. A) Biofuels B) Extraction of lipids from marine microorganisms

Shaikh Anjuman

5. Enzymes in food industries Antibacterial activity of Tinospora cordifolia

Soregaonkar Samir

11

2007-2008

1. Extraction of polyunsaturated fatty acids from microbial sources

Agarwal Aditi, Manjulatha

2. RAPD analysis in Withania somnifera Jadhav Vipra

3.

Extraction, isolation and identification of active ingredient from Abutilon pannosum and Canthium parviflorum and testing their

Phatan Ejaj

4. Comparitive studies on probiotic organisms

Dixit Gauri

5. Isolation & identification of salt tolerant nitrogen fixing microorganisms

Jadhav Girish G

2008-2009

1. Screening & characterization of bacteria with fibrinolytic activity from marine source

Hoda Sadal Aayadi, Maryam Ranjpour

2. Antifungal activity of Murraya kurraya koenigii

Majumder Debamita

3. Production of omega-3 fatty acids from marine microbial sources

Nirmal Pallavi

4. DNA profiling & antimicrobial activity of medicinal plants

Pawar Joyti & Anugade Sheela

5. Production of omega-3 fatty acids by micro- organisms & optimization of cultural conditions

Tiwari Neha

6. Isolation & characterization of marine microorganism producing poly-saturated fatty acid

Nikam Kapil

2009-2010

1. Screening of marine microorganisms for protease and lipase enzymes

Rachana

2. Elisha 3.

Screening of bioactive compounds from marine microbial sources

Ratna ganguli 4.

Sonal Majrekar

5. Shruti Shree

12

Projects completed by the students other than RGITBT:

5. Research profile

Ph. D. program

Sr. No. Name of

the

student

Topic of research Registration

year

Guide

1. Vipra V. Jadhav

Biotech potential of marine organisms

and yeasts for PUFA

2008 Dr. Mrs.

Rama Bhadekar 2. Dipti S.

Salunkhe Studies on

polyunsaturated 2008

Sr. no. Student name Theme of research Year M.Sc. Microbiology (Y. M. College, Pune)

1. Rohit Ajit Alasandkar Antimircobial activity of Tinospora cordifolia and isolation of its active principles

2007-08

2. H Abhishek Nair Study of lipid production by Rhodotorula rubra.

2007-08

3. Shantinath Khot Antimicrobial activity of Nothapodytes foetida and phytochemical studies

2007-08

4. Snehal Patil Screening of PUFA producing microorganisms from Indian Ocean

2008-09

5. Sandip Mulik Optimization of parameters for PUFA production in marine microorganisms from Indian Ocean.

2008-09

B.E. Chemical Engineering

1. Vishal Taneja

Studies on culture conditions of Rhodotorula rubra for biomass production

2007-08

2. Sunny Arora 2007-08

13

fatty acids from marine

microorganisms and oleaginous yeasts

Papers presented

R. Bhadekar, N. Meti, V. Jadhav, G. Patil and D.P.Nerkar In vitro cultivation and

RAPD analysis in Withania somnifera Paper presented at Global Ayurved

Conference organized by Bharati Vidyapeeth University , Pune, between January 9-

11, 2009.

BR Ehsan & N K Bipinraj, Antimicrobial activity of the ethanolic extract of B.

Laciniosa leaf, stem, fruit and seed. Afr. J Biotechnol., Global Ayurved Conference

and Health Expo , 2009.

Posters presented (International conferences)

Ejaj Pathan, D. P. Nerkar and R. K. Bhadekar. Studies on phytochemicals from

Abutilon pannosum, (Kasali). Poster presented at Indo-Swedish International

Conference on “Biotechnology for Sustainable Development” organized at NCL,

Pune between Jan. 7-9, 2008.

R. Bhadekar , N. Meti, M. Falaki Khalilsaraie, R. Alasandkar, and D. P. Nerkar,

Antimicrobial activity of stem, leaf and callus induced from leaves of Tinospora

cordifolia. Poster presented at Global Ayurved Conference organized by Bharati

Vidyapeeth University , Pune, between January 9-11, 2009.

Pallavi Nirmal, Vipra Jadhav, D. P. Nerkar and R. K. Bhadekar. Poster presented at

50th International Conference of Association of Microbiologists of India held at

NCL, Pune, between Dec. 15 -18, 2009

Laboratory manuals

Rama Bhadekar, Deepti Samarth and Dipti Salunkhe. Laboratory manual of

Microbiology for M.Sc. Biotechnology (Sem. I and II), Rajiv Gandhi Institute of I.T

and Biotechnology, 2010.

Rama Bhadekar, Vipra Jadhav and Deepti Samarth. Laboratory manual of Genetic

Engineering for M.Sc. Biotechnology (Sem. III and IV), Rajiv Gandhi Institute of I.T

and Biotechnology, 2010.

14

Recent Publications

Dipti Salunkhe, Neha Tiwari, Sandeep Walujkar, Rama Bhadekar. (2010) Halomonas

sp. Nov., an EPA producing mesophilic marine isolate from the Indian Ocean. Polish

Journal of Microbiology (In press)

Vipra Vijay Jadhav, Manoj Mahadev Jamle, Pravin Dnyaneshwar Pawar, Mayur

Nimbadas Devare, Rama Kaustubh Bhadekar. Fatty acid profiles of PUFA producing

Antarctic bacteria: correlation with RAPD analysis. Annals of Microbiology (In

Press) DOI: 10.1007/s13213-010-0114-4.

Girish Gajanan

Jadhav, Dipti

Sambhaji Salunkhe, Devdas Punjaji Nerkar, Rama Kaustubh Bhadekar (2010)

Isolation and characterization of salt-tolerant nitrogen-fixing microorganisms from

food. EurAsian Journal of Biosciences, 4, 33 – 40.

Girish Gajanan Jadhav, Dipti Sambhaji Salunkhe, Devdas Punjaji Nerkar, Rama

Kaustubh Bhadekar (2010) Novel Staphylococcus sp isolated from wall scrapings of

historical building in India. Annals of Microbiology, 60, 197 – 201.

BR Ehsan, V Awad & N K Bipinraj, Antimicrobial activity of the ethanolic extract of

B. Laciniosa leaf, stem, fruit and seed. Afr. J Biotechnol., 8 (2009) 3565-3567.

E A Singh, SY Kamble and N K Bipinraj, Medicinal plants from newly reported

devraies (sacred groves) in Pune district. Ind. J Trad. Knowledge(in press).

A Z Abubaker, B R Ehsan and N K Bipinraj, Antimicrobial activity of six Indian

spices, ICFAI J.biotechnol (in press).

S Mukherjee, N M pawar, S Thopte, B Nagarkar, A D Bhujbal, R Chowdhury, P

Raina, N K Bipinraj & P K Pawar, Evaluation of anti- microbial potential of

Vayasthapana rasayana formulation on some pathogenic bacteria, J Toxicol Herbal

Med (in press).

Grants received

Ongoing project on “Microbial production of Omega-3 fatty acid” in collaboration

with IRSHA funded by DBT, Govt. of India.

Year Amount

15

Rs. 2 Lakhs for Molecular characterization of Indian strawberry cultivars, In-house

project

6. Executive summary

2008 – 09 23. 32 Lakhs

2009 – 10 3.0 Lakhs

2010 - 11 4.82 Lakhs

(awaited)

Sr. no. Category Accomplishments

1. No. of publications 14

2. T.Y. B.Sc. Reviews guided 50

3. No. of M.Sc. students guided for projects from 2006

43

4. No. of Ph. D. students 2

4. Papers / Posters presented 3 Papers, 3 Posters

5. Conferences/Workshops attended 9 conferences, 8Workshops

6. Collaborations

1. IRSHA, BVDU, Pune

2. NCCS, Pune

3. COEP, Pune

7. Grants received 28 Lakhs

16

7. Library profile

Collection of library books until 2009-10

Department Books

(2008-09) Books added

(2009-10) Total No. of

Books

Microbial Biotechnology 356 22 378

Subscribed Journals & Magazines

2009-2010 Sr. No. Journals

1 Current Science

2 The ICFAI Journal of Life sciences

3 The ICFAI Journal of Science & Technology

5 The ICFAI Journal of Biotechnology

6 The ICFAI Journal of Environmental Sciences

7 The ICFAI Journal of Genetics & Evolution

8 Journal of Biosciences

9 Bio Research International

10 Journal of Scientific and Industrial Research

11 Indian Journal of Experimental Biology

12 Research Journal of Biotechnology

14 Indian Journal of Biochemistry & Biophysics

15 Indian Journal of Biotechnology

16 Trends in Biotech

Magazines

1 Scientific American (India)

3 Chemical Weekly

4 Bio-spectrum

5 Down to Earth

7 Proteomics

8 Medicinal and Aromatic Plants Abstracts