Department of

BiotechnologyAbout Us: The Department of Biotechnology was established in 2001 under Faculty of

Science and is currently under School of Biological Sciences. The MSc and PhD

programs of the department prepare quality human resource for research in academic

and industrial setting. The department provides comprehensive and quality educational

and research avenues to the students through its programs. The department’s current

research areas include plant biotechnology with focus on development of transgenic

plants and disease biology with emphasis on cancer biology and ion channel

physiology.

Mission: The Department of Biotechnology is committed for providing research and

educational programs of international standards to the students. We seek to provide rich

educational experience to the students that includes rigorous theoretical courses and

practicals to understand the concepts of biology. In addition, our department constantly

updates students with recent technological and technical advances in the field of

biotechnology. The ultimate objective of our department is to train students to use

biological concepts and develop innovative technologies for the benefit of mankind.

Vision: The department envisions to develop a robust interdisciplinary research

environment while maintaining personalized research environment to perform research

of national and international prominence. One of the major focus of the department will

be to effectively integrate the educational and research programs to deliver maximum

output in terms of student training and career development. The department foresees to

expand the research focus to areas including cancer biology, cell biology, DNA

replication and repair, stem cell research, gene expression, molecular medicine and

plant molecular biology. The goal of the department is to develop next generation young

scientists who will make break through discoveries and provide cost effective solutions

to various for human problems through creative and innovative research.

Research Laboratory Smart Classroom Plant Tissue Culture

Facu

ltyProf Subodh K. Jain

Head, Dept. of BiotechnologyProfessor, Dept. of Zoology PhD, BiochemistryEmail: subjain@gmail.com

Research interests: Molecular biology and stress physiology

Selected Publications

Bharat Neekhra, Divya Pandey, Meeta Mishra, Subodh Kumar Jain Molecular Marker

Approach In Honey Bee: A Review. Int J Pharm Bio Sci 2012 July; 3(3): (B) 261 – 271

(Impact Factor 0.47)

Jain, Subodh Kumar and Zelena Dora: Gender specific influence of endogenous glutamate

release on stress-induced fear in rats. (Accepted for publication on 5.11.2010 in Endocrine

Regulation, Bratislava, Slovak Vol. 45, 13-21 2011 ISSN: 0344-0338, Impact factor: 1.08)

Role and clinical significance of lymphocyte mitochondrial dysfunction in type 2 diabetes

mellitus. Translational Research (2011) vol.158 No.6, 344-359 (Impact Factor 2.763)

Mishra, P.K.; Raghuram, GV; Bhargava, A; Ahirwar, A; Samarth, R; Upadhyaya, R; Jain,

Subodh Kumar; Pathak, Neelam: In vitro and in vivo evaluation of the anticarcinogenic and

cancer chemopreventive potential of a flavonoid-rich fraction from a traditional Indian herb

Selaginella bryopteris. British Journal of Nutrition (2011), 106, 1154-1168. (Impact Factor

3.45)

Raghuram, G.V.; Pathak, N.;Jain, D.; Panwar, H.;Pandey, H.; Jain, Subodh Kumar Jain,

Mishra, P.K.: Molecular mechanisms of isocyanate induced oncogenic transformation in

ovarian epithelial cells. Reproductive Toxicology 30 (2010) 377-386. (Impact factor 2.957)

Current Research Grants

RAPD-PCR based biomarker characterization and genetic diversity of stored grain pest C.

maculatus and C. chinentis of Madhya Pradesh. Funded from MPCST

Design and validation of a clinical translational algorithm for molecular risk-assessment of

hepatocellular carcinoma. Funded from UGC

Students

Dr. Bharat Neekhra, Postdoctoral fellow

Abdul Anvesh Mansoori, PhD candidate

Facu

ltyDr Chandrama Prakash Upadhyaya

Assistant Professor Stage II

PhD, Botany

Email: cpupadhyay@gmail.com

Research interests: Plant Molecular Biology, Plant Functional Genomics, Stress Physiology

and Nutrigenomics

Selected Publications

Chandrama P Upadhyaya, M A Gururani, Ram Prasad and Ajit Verma (2013) A Cell Wall

Extract from Piriformospora indica Promotes Tuberization in Potato (Solanum tuberosum L.)

Via Enhanced Expression of Ca+2 Signaling Pathway and Lipoxygenase Gene. Appl

Biochem Biotechnol. Volume 170, Issue 4, pp 743-755. (Impact factor 2.6)

Mayank A Gururani, Chandrama Prakash Upadhyaya, Reto J strasser, & Se W

Park (2013) Evaluation of abiotic stress tolerance in transgenic potato plants with reduced

expression of PSII manganese stabilizing protein. Plant Science,198. 7–16. (Impact Factor

3.01)

Mayank A Gururani, Chandrama Prakash Upadhyaya, Jelli Venkatesh, V Baskar and Se

Won Park (2013) "Plant growth promoting rhizobacteria enhance abiotic stress tolerance in

Solanum tuberosum through inducing changes in the expression of ROS scavenging

enzymes and improved photosynthetic performance". Journal of Plant Growth

Regulation, June 2013, Volume 32, Issue 2, pp 245-258. (Impact Factor 2.91)

Mayank A Gururani, Chandrama Prakash Upadhyaya, Jelli Venkatesh, Reto J strasser, &

Se Won Park (2012) Physiological and biochemical responses of transgenic potato plants

with altered expression of PSII manganese stabilizing protein indicates its role in potato

tuberization. Plant Physiology and Biochemistry 58, 182–194. (Impact Factor 3.0)

Chandrama Prakash Upadhyaya, Jelli Venkatesh, Mayank Anand Gururani, Leonid Asnin,

Kavita Sharma, and Se Won Park (2011) Transgenic potato overproducing L-ascorbic acid

resisted an increase in methylglyoxal under salinity stress via maintaining higher reduced

glutathione level and glyoxalase enzyme activity. Biotech letters, 2011 Nov;33(11):2297-307.

(Impact Factor 1.61)

Current Research Grants

Isolation, characterization and over-expression of CaM, a calcium dependant protein in

tomato. Funded from DBT under RGYI project.

Investigating and probing RBC-Endothelial cell interaction through ex-vivo cerebral malaria

model to develop adjuvant therapy for malaria pathology. Funded from DBT under twinning

programme in collaboration with IIT Guwahati.

Students

Deepak Baghri, PhD Candidate

Facu

ltyDr Rajaneesh Anupam

Assistant Professor Stage II PhD, Biochemistry and Molecular BiologyEmail: rajaneeshanupam@gmail.com

Research interests: Cancer biology, viral host-protein interactions, anti cancer drugs

Selected Publications

Doureiri R*, Anupam R*, Kvaratskhelia M, Green-Church K, Lairmore MD, Green GL.

(2012) Comparative Host Protein Interactions with HTLV-1 p30 and HTLV-2 p28: Insights into Difference in Pathobiology of Human Retroviruses. Retrovirology. 9:64 (Impact factor

5.66) (* equal authors) Highly accessed

Anupam R, Datta A, Kesic M, Green-Church K, Shkriabai N, Kvaratskhelia M, Lairmore

MD. (2011) Human T-lymphotropic Virus Type 1 p30 Interacts with REGγ and Modulates

ATM (Ataxia Telangiectasia Mutated) to Promote Cell Survival. J Biol Chem. 286, 7661-8.

(Impact factor 4.65)

Anupam R, Denapoli L, Muchenditsi A, Hines JV. (2008) Identification of neomycin B-

binding site in T box antiterminator model RNA. Bioorg Med Chem. 16, 4466-70. (Impact

factor 3.15)

Anupam R, Nayek A, Green NJ, Grundy FJ, Henkin TM, Means JA, Bergmeier SC, Hines

JV. (2008) 4,5-Disubstituted oxazolidinones: High affinity molecular effectors of RNA

function. Bioorg Med Chem Lett. 18, 3541-4. (Impact factor 2.43)

Means J, Katz S, Nayek A, Anupam R, Hines JV, Bergmeier SC. (2006) Structure-activity

studies of oxazolidinone analogs as RNA-binding agents. Bioorg Med Chem Lett. 16, 3600-

4 (Impact factor 2.43)

Current Research Grants

Role of HTLV p30 and REG-gamma interaction in DNA damage regulation. Funded from

UGC under start-up grant scheme.

Students

Priyanka Namdev, PhD Candidate

Facu

ltyDr Anamika Bhargava

Assistant Professor PhD, Medical SciencesEmail: abhargava@dhsgsu.ac.in

Research interests: Voltage gated calcium channels in physiology and pathophysiology, Cell

signaling.

Selected Publications

TingTing Hong, Huanghe Yang, Shan-Shan Zhang, Hee Cheol Cho, Mariya Kalashnikova,

Baiming Sun, Hao Zhang, Anamika Bhargava, Michael Grabe, Jeffrey Olgin, Julia Gorelik,

Eduardo Marban, Lily Y Jan & Robin M Shaw. Cardiac BIN1 folds T-tubule membrane,

controlling ion flux and limiting arrhythmia. Nature Medicine (2014) doi:10.1038/nm.3543.

(Impact factor 24.03)

Anamika Bhargava, Xianming Lin, Pavel Novak, Kinneri Mehta, Yuri Korchev, Mario

Delmar and Julia Gorelik. Super-resolution scanning patch-clamp reveals clustering of

functional ion channels in the adult ventricular myocyte. Circ Res. 2013 Apr 12; 112(8):

1112-20. (Impact factor 9.5)

Singh Anamika, Hildebrand ME, Garcia E and Snutch TP. TRPC antagonist SKF 96365 is

a potent blocker of T-type calcium channels. Br J Pharmacol. 2010 Jul;160(6):1464-75.

(Impact factor 4.4)

Singh Anamika, Gebhart M, Fritsch R, Sinnegger-Brauns M J, Poggiani C, Hoda J C,

Engel J, Romanin C, Striessning J and Koschak A. Fine-tuning of voltage- and Ca2+-

dependent gating of CaV1.3 L-type calcium channels by alternative splicing of a C-terminal

regulatory domain J Biol Chem. 2008 Jul 25;283(30):20733-44. (Impact factor 4.77)

Singh Anamika, Hamedinger D, Hoda J C, Gebhart M, Koschak A, Romanin C &

Striessning J. C-terminal modulator controls Ca2+-dependent gating of CaV1.4 L-type Ca2+

channels. Nat Neurosci. 2006 Sep;9(9):1108-16. (Impact factor 15.53)

Current Research Grants

Role of C-terminus of T-type voltage gated calcium channels in regulating channel activity

and expression”. Funded from DBT under under Bio-CARe scheme.

Students

Divya Rawat, PhD Candidate

Departmental Facilities

DNA Sequencer (CIF) Flow Cytometer (CIF) Plant Tissue Culture

Fridge and -20 Freezer FermentorRotary Shaker

Shaking IncubatorAutoclaveMicroscopePCR Machine

Laminar Air FlowCooling Centrifuge Electronic Balance

Central Facilities

Following instruments are available through the state-of-the-art Central

Instrumental Facility (CIF) of Dr. Hari Singh Gour Vishwavidyalaya:

Confocal Microscope

Atomic Force Microscope

Scanning Electron Microscope (SEM)

Transmission Electron Microscopy (TEM)

Micro-Raman spectrophotometer

Nuclear magnetic resonance (NMR)

Real Time PCR

Ultracentriguge

Nano spray dryer

Fourier transform infrared spectroscope (FTIR)

High performance thin layer chromatograph (HPTLC)

Courses Offered

The Department of Biotechnology currently offers the following

postgraduate courses. The admission to both the courses is

through national entrance exam conducted by Dr. Hari Singh Gour

Vishwavidyalaya during May-june every year.

MSc Biotechnology

Duration: 4 semesters

Total seats: 20

PhD Biotechnology

Duration: 3-4 years

Total seats: Variable depending on the availability with the faculty