department of biochemistry foundation module phase - 1
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Department of Biochemistry Foundation Module Phase - 1. Lipids. Soluble in non-polar solvents and insoluble in polar solvents. Lipids are not polymers. Fatty acids. They are basic building blocks of lipids(simplest lipids). Fatty acids are carboxylic acids. - PowerPoint PPT PresentationTRANSCRIPT
Department of BiochemistryFoundation Module Phase - 1
Lipids Soluble in non-polar solvents and insoluble in polar solvents. Lipids are not polymers.
Fatty acids •They are basic building blocks of lipids(simplest lipids).•Fatty acids are carboxylic acids.
Q.Fatty Acids are amphipathic molecules. Why?
A.Because they have both polar(hydrophilic) and non-polar(hydrophobic) portions
in their structure
Classification of fatty acids
Q. What is the chemical difference between saturated and unsaturated fatty acids?
A. Saturated fatty acids contain only single
bonds between carbons, whereas unsaturated fatty acids contain at
least one double bond.
Corn oil contains 86% polyunsaturated fatty acidsTherefore is a liquid at room temperature.
Olive oil contains monounsaturated fatty acids. Therefore is a liquid at room temperature, likely to solidify when refrigerated.
Saturated fatty acids (tropical oils, animal fats) are solids, or nearly solids at room temperature.
Unsaturated fats are preferred over saturated fatty acids for nutritional reasons.
Q.Would you expect a deep sea fish (living in cold water) or a shallow water fish (living in warmer water) to have a higher percentage of unsaturated fatty acids in its tissues?
A. deep sea fish
Q1.Give some examples for simple lipids
Q2.Find out some common examples for complex lipids
Fatty acids + Alcohol
Fatty acids + Alcohol+ X
The degree of saturation of a fatty acid affects particularly the fatty acid
melting point.
Q3.Unsaturated fatty acids have lower melting points than saturated fatty acids. Why is that?
Melting Points and Solubility in Water of Fatty Acids
Solubility in H2O
Chain Length
Melting Point
Q. What are
essential fatty
acids?
A. Fatty acids which have to be obtained from food, as human cells have no appropriate enzymes required to produce them internally.
Q4.What are the two families of essential fatty acids?
Good sources of ‘omega-3 fatty acids’
• Oily fish (salmon, mackerel, sardines, tuna) • Pumpkin seeds, sesame seeds• soybean oil
Good sources of ‘omega-6 fatty acids’•Most vegetable oil, Sunflower oil, Corn oil, Soybean oil•Cotton seeds oil•Pumpkin seeds•Nuts and cereals•Poultry, eggs•Avocado
Q5.Why are essential fatty acids good for
you?
Essential fatty acids are used by every cell in your body.
Provide energy
Structural components of biological membranes.
synthesize bile acids that aid
lipid solubilization
Serve as vitamins and hormones.
Lipids have four major functions which are physiologically important for human.
Glycerides (acylglycerols) are esters formed from glycerol and fatty acids.
Glycerides
Q6.What elements are found in lecithin and
sphingolipids?
Q7. Identify a-j
Q8.What are Eicosanoids?
B
A
Q9.Name the enzymes A& B.State the mechanisms by which aspirin and ibuprofen inhibit PGH2 Synthesis.
Steroids
•General structure of a steroid – four rings
Q10.What are the derivatives of steroids?
• Most abundant steroid in the body• Add methyl CH3- groups, alkyl chain,
and -OH to steroid nucleus
Cholesterol
Cholesterol in the Body,
• Cellular membranes• Myelin sheath, brain,
and nerve tissue• Bile salts• Hormones• Vitamin D
Q11 . Identify each lipid as: (1) phospholipid (2) steroid (3) triglyceride (4) sphingolipid
A. Cholesterol B. Glycerol, 2 fatty acids, phosphate, and choline C. Sphingosine, fatty acid, phosphate, and choline D. Estradiol E. Bile salts F. Most of plasma membranes
The diagram represents a phospholipid molecule
(i) A
(ii) B
(iii) C
Q12.Name the following parts of the molecule
1.Saponification Value
What are the main ingredients in your soap?
• One test tube is filled with Sunflower oil (A). • Coconut oil is placed in a second test tube.(B)• Add few drops of Hubl’s iodine for each solution. • Test tubes are shaken. • After some minutes a spatula full of starch powder is added
to each of the two test tubes. • The test tubes are again shaken.
A B
Q13.what is the observation?
Foods prepared with edible oil develop a kind of smell and undergo changes in taste after some time. What is the reason for this?
Rancidity
Hydrolytic Oxidative
Partial hydrolysis of TAG Oxidation of UFA @double bond positions.
FFA Glycerol(mono/di) Peroxides
Aldehyde Ketones
Objectionable odour + Taste
Health issues associated with lipids
Trans fat•Unsaturated fat with trans-isomer fatty acid.•Not essential fatty acids
•Naturally found in animal products where they are produced by bacteria in the gut of ruminant animals (eg: cow, goat, sheep) •Therefore some trans fatty acids are present in meat, milk and other dairy products.•These are mostly C18 monounsaturated fatty acid (vaccenic acid).• Daily intake of 5g trans fat increases the risk of heart disease by 25%
Trans fatty acids are commonly found in…..
Sun flower oil
How are trans fats made artificially?
Trans fats are made through the chemical process called hydrogenation of vegetable oils. Hydrogenation solidifies liquid oils and increases the shelf life and the flavour stability of oils and foods that contain them.
Margarine
ANSWERS
a.Fats: Fatty acids + Glycerol
b. Waxes: Fatty acids + Long chain alcohol
Phosphoric acid residue
Phosphoric acid residueCholine
Carbohydrate
Alcohol
Glycerol
Glycerol
Sphingosine
Fatty acids
Fatty acids
Fatty acids
Fatty acids
a.Phospholipidex:1.Glycero phospholipid
ex: Lecithin
2.Sphingophospho lipid
b.Glycolipids(Glycosphingo lipid)
XAlcoholFatty acidsExample
Presence of one or more double bonds in hydrocarbon chain of unsaturated fatty acids results “ BENDS” in the molecules. These molecules do not stack well. As a result of that inter-molecular interactions become much weaker than saturated fatty acids leading to lower melting points.
1. ω-3 (or omega-3 or n-3)2. ω-6 (omega-6, n-6.)
• Alpha-linolenic acid – a polyunsaturated omega-3 fatty acid
• Linoleic acid -an unsaturated omega-6 fatty acid.
• Control immune system, blood pressure, nervous system, inflammatory reactions, blood clotting and hormonal functions.
• Regulate a large number of mechanisms including increasing the fluidity of cell membranes and improving the ability of selective permeability and help to keep toxin out and bring nutrients into cells.
• Influence the activation of cell genes and act as second messengers and produce good eicosanoids.
• Furthermore, a diet rich in EFAs can be helpful in many diseases( Rheumatoid arthritis, Inflammatory bowel disease)
Lecithin
• a: Oligosaccharide• b: Glycoprotein• c: Glycolipids• d: Head of phospholipid• e:Tail of phospholipid• f: Phospholipid bilayer• g: Filament• h:Peripheral protein• i: Cholesterol• j: Transmembrane protein
Eicosanoids are a family of powerful, hormone-like compoundsproduced in the body from essential fatty acids . . .
• They have specific effects on target cells close to their site of formation.
• They are rapidly degraded, so they are not transported to distal sites within the body.
• Examples: prostaglandins, prostacyclins, thromboxanes, leukotrienes, epoxyeicosatrienoic acids (EETs).
• Roles in inflammation, fever, regulation of blood pressure, blood clotting, immune system modulation, control of reproductive processes & tissue growth, sleep/wake cycle regulation.
A: LipoxygenaseB:PGH2 synthase
Aspirin and Ibuprofen (a Non-steroid anti inflammatory drug) Inhibit PGH2 Synthase enzyme.
Note: Aspirin irreversibly acetylates and thus inactivates the enzyme whereas the action of Ibuprofen is reversible.
• Steroid hormones• Cholesterol• Vitamin D• Bile
A. steroidB. phospholipidC. sphingolipidD. steroidE. steroidF. phospholipid
2 Fatty acidsGlycerolPhosphate group & N- base
Discussion and background:
Sunflower oil contains unsaturated fatty acids. Iodine adds across the double bonds in an unsaturated bond. Coconut oil consists mainly of glycerides from saturated fattyacids (e.g. palmitic and lauric acid).
Observation:The sample containing Coconut oil turns intensely blue(iodine-starch complex).In Sunflower oil all the iodine will be consumed.Consequently no colour change can be observed after the addition of starch.