david sampson school of electrical, electronic, & computer engineering

David Sampson

School of Electrical, Electronic,& Computer Engineering

FABLS, Macquarie University, 21 February 2005FABLS, Macquarie University, 21 February 2005 OBEL CapabilitiesOBEL Capabilities

Engineering capabilities:Optical engineering

Photonics – optical fibre technology

Building (portable) instruments

Clinical measurements














Science capabilities:Optical coherence tomography

Tissue optics – light scattering

In vivo imaging

Human upper airway

Skin cancer – melanoma

Two-photon microscopy

Gross morphology

In vivo, real-time

Few millimetre depths

Courtesy of M. Stante, P. Cappugi, University of Florence




In vivo imaging

Human upper airway



Mie scattering




In vivo imaging

Human upper airway



Optical coherencetomography

Confocal reflectancemicroscopy

Two-photon autofluorescencemicroscopy

Arch. Dermatol. 137, 923-929, 2001 J. Biomed. Opt. 8(3), 432–439, 2003 Opt. Lett. 28, 2064-2066, 2003




In vivo imaging

Human upper airway



Oesop

hagus

External nare

s

Nasal cavity

Nasopharynx

Hyp

opharynx

Oro

phayrnx

Sleep apnoea

Transverse section as probe is scanned along airway




In vivo imaging

Human upper airway



Melanoma in-situ


Two-photon autofluorescence microscopyTwo-photon autofluorescence microscopy

Depth

Phosphate-buffered saline

Optical clearing agent

•Contrast/resolution/penetration in thick samples – all methods limited by scattering

•Can you reduce scattering by a biocompatible optical clearing agent?

•Investigate optical clearing agents – mechanisms, dynamics, specificity

•What else can we do to enhance thick tissue/in vivo imaging?

•Realise an in vivo instrument and establish what can it do?


FABLS – what would make a difference?FABLS – what would make a difference?

• Access to life sciences expertise– make possible interdisciplinary research– solve the right problems

• Attract more graduate students

• Training opportunities for researchers– in labs– high-level postgrad lectures/courses

david sampson school of electrical, electronic, & computer engineering

Documents

airwayscience capabilities

thick samples

thick tissuein

right problemsattract