dart-based linkage map of the solanum tuberosum ssp andigena accession lop-868 (cip 702853) cultivar...
TRANSCRIPT
![Page 1: DArT-based linkage map of the Solanum tuberosum ssp andigena accession LOP-868 (CIP 702853) Cultivar Name : Alca Tarma Chromosomes I to XII Maria V. Caraza](https://reader035.vdocuments.us/reader035/viewer/2022081513/56649e4d5503460f94b43b19/html5/thumbnails/1.jpg)
DArT-based linkage map of the
Solanum tuberosum ssp andigena accession LOP-868
(CIP 702853)Cultivar Name : Alca Tarma
Chromosomes I to XII
Maria V. Caraza SalasElisa J. Mihovilovich CastroMariela Aponte VilladomaMerideth Bonierbale
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Notes
Population size =174 dihaploid progenies derived from haploidization of LOP 868 with haploid inducer lines IVP-101 and 596001.4 of Solanum phureja Juz. et Bukasov
Total DArT markers mapped = 521 Mean DArT markers per chromosome = 43 Number of SSR markers mapped = 45 Mean SSR markers per chromosome = 4 Additionally:
A major gene denoted Rladg for resistance to PLRV identified in this accession mapped on chromosome V (Velásquez et al. 2007)
An AFLP marker P13M34.453 mapped close to the major gene for PLRV resistance
Position of QTL for late blight resistance identified by association mapping in a Solanum t. andigena-derived B1 Population on chromosomes I, II, III; and IX
Total length of the tetraploid map = 1211.6 cM
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Linkage map construction
The genetic map was constructed using the statistical program TetraploidMap (Hackett and Luo 2003)
Marker order was established by the simulated annealing algorithm of TetraploidMap at starting T = 10, a cooling factor of = 0.85 and M = 100n random changes at each T°
Chromosome assignment and polarity was established by mapping SSR markers and using “sol genomics network” maps; Potato meta-QTL consensus map and Solanum phureja diploid map 2010, as reference http://solgenomics.net/
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References
Hackett CA, Luo ZW (2003) TetraploidMap: construction of a linkage map in autotetraploid species. J Hered 94(4):358–359
Velásquez AC, Mihovilovich E, Bonierbale M. (2007) Genetic characterization and mapping of major gene resistance to potato leafroll virus in Solanum tuberosum ssp. andigena. Theor Appl Genet 114 (6):1051-1058
![Page 5: DArT-based linkage map of the Solanum tuberosum ssp andigena accession LOP-868 (CIP 702853) Cultivar Name : Alca Tarma Chromosomes I to XII Maria V. Caraza](https://reader035.vdocuments.us/reader035/viewer/2022081513/56649e4d5503460f94b43b19/html5/thumbnails/5.jpg)
Chromosome I
QTL for late blight resistance found in B1 population by association mapping
![Page 6: DArT-based linkage map of the Solanum tuberosum ssp andigena accession LOP-868 (CIP 702853) Cultivar Name : Alca Tarma Chromosomes I to XII Maria V. Caraza](https://reader035.vdocuments.us/reader035/viewer/2022081513/56649e4d5503460f94b43b19/html5/thumbnails/6.jpg)
Chromosome II
QTL for late blight resistance found in B1 population by association mapping
![Page 7: DArT-based linkage map of the Solanum tuberosum ssp andigena accession LOP-868 (CIP 702853) Cultivar Name : Alca Tarma Chromosomes I to XII Maria V. Caraza](https://reader035.vdocuments.us/reader035/viewer/2022081513/56649e4d5503460f94b43b19/html5/thumbnails/7.jpg)
Chromosome III
QTL for late blight resistance found in B1 population by association mapping
![Page 8: DArT-based linkage map of the Solanum tuberosum ssp andigena accession LOP-868 (CIP 702853) Cultivar Name : Alca Tarma Chromosomes I to XII Maria V. Caraza](https://reader035.vdocuments.us/reader035/viewer/2022081513/56649e4d5503460f94b43b19/html5/thumbnails/8.jpg)
Chromosome IV
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Chromosome V
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Chromosome VI
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Chromosome VII
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Chromosome VIII
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Chromosome IX
QTL for late blight resistance found in B1 population by association mapping
![Page 14: DArT-based linkage map of the Solanum tuberosum ssp andigena accession LOP-868 (CIP 702853) Cultivar Name : Alca Tarma Chromosomes I to XII Maria V. Caraza](https://reader035.vdocuments.us/reader035/viewer/2022081513/56649e4d5503460f94b43b19/html5/thumbnails/14.jpg)
Chromosome X
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Chromosome XI
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Chromosome XII