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Page 1: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research
Page 2: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Cynthia Skema, Ph.D. University of Pennsylvania Cindy Skema is a botanist at the Morris Arboretum of University of Pennsylvania. She studies the evolution of plants in the field, herbarium, and lab, and enjoys communicating about plant science to the public. Dr. Skema earned her Ph.D. at Cornell University, and has worked in Madagascar and New Zealand.

Page 3: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Quick Guide to the

Botany of Beer Cindy Skema, Botanical Scientist

Page 4: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Most good things come from plants. Beer is one of them.

Page 5: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Most good things come from plants. Beer is one of them.

starch fermenter flavor/ preservative

= beer + + water +

Page 6: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

starch fermenter flavor/ preservative

= beer + +

Most good things come from plants. Beer is one of them.

water +

Page 7: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Barley: What is it?

© Champagne Whiskey

Page 8: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Barley: What is it?

• Hordeum vulgare

© Champagne Whiskey

Page 9: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Barley: What is it?

• Hordeum vulgare

• grass! Poaceae (wheat, rye, oats)

© Champagne Whiskey

Page 10: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Barley: Where is it?

• distribution: wild

• domestication: non-shattering (2 loci)

© University of Minnesota Institute of the Environment

Page 11: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Barley: Where is it?

• distribution: wild vs. cultivated

• domestication: non-shattering (2 loci)

© University of Minnesota Institute of the Environment

Page 12: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Barley: What we use…

Page 13: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Barley: What we use…

ovary

Page 14: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Barley: What we use…

stamens

1 2

3

1 2

3

ovary

Page 15: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Barley: What we use…

stamens

1 2

3

1 2

3

ovary palea

lemma

Page 16: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

• ovary of grass flower fruit, or ‘grain’

Barley: What we use…

stamens

1 2

3

1 2

3

ovary palea

lemma

Page 17: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

• ovary of grass flower fruit, or ‘grain’

• malting (fruit+)

– dry store (break dormancy)

– steep

– germinate

– dry (kill)

Barley: What we use…

endosperm

embryo

Page 18: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

• ovary of grass flower fruit, or ‘grain’

• malting (fruit+)

– dry store (break dormancy)

– steep

– germinate

– dry (kill)

Barley: What we use…

endosperm

root

scutellum embryo

shoot

Page 19: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

• ovary of grass flower fruit, or ‘grain’

• malting (fruit+)

– dry store (break dormancy)

– steep

– germinate

– dry (kill)

Barley: What we use…

endosperm

aleurone

pericarp/testa

scutellum embryo

root

shoot

Page 20: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

• ovary of grass flower fruit, or ‘grain’

• malting (fruit+)

– dry store (break dormancy)

– steep

– germinate

– dry (kill)

Barley: What we use…

endosperm

aleurone

pericarp/testa

husk (lemma + palea)

scutellum embryo

root

shoot

Page 21: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

• ovary of grass flower fruit, or ‘grain’

• malting (fruit+)

– dry store (break dormancy)

– steep (add water)

– germinate (modification)

– dry (kill)

Barley: What we use…

endosperm

aleurone

pericarp/testa

husk (lemma + palea)

scutellum embryo

root

shoot

Page 22: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

• ovary of grass flower fruit, or ‘grain’

• malting (fruit+)

– dry store (break dormancy)

– steep (add water)

– germinate (modification)

– dry (kill)

Barley: What we use…

endosperm

aleurone

pericarp/testa

husk (lemma + palea)

scutellum embryo

root

shoot

starch

Page 23: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Hops: What is it?

Page 24: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Hops: What is it?

• The Hemp Family, Cannabaceae

– Humulus, 2 spp.

• Humulus lupulus, 5 varieties

–Humulus lupulus var. lupulus

Page 25: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Hops: How is it?

• natural history:

– temperate perennial, twining herb (not a vine!) supported by trees, wind-pollinated

– dioecious, with heterogametic sex chromosomes

Page 26: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Hops: What we use...

• brewer’s ‘cone’ = botanist’s ‘inflorescence’

– bracts, bracteoles

Page 27: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Hops: What we use...

• brewer’s ‘cone’ = botanist’s ‘inflorescence’

– bracts, bracteoles

Page 28: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research
Page 29: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research
Page 30: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Hops: What we use...

• brewer’s ‘cone’ = botanist’s ‘inflorescence’

– bracts, bracteoles

– it’s all about the glands lupulin resin (lupulone, humulone acids)

Page 31: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Hops: What we use...

© Nagel et al., 2008, Plant Cell

strig

glands

bracteole

bract

Page 32: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Hops: What we use...

© Nagel et al., 2008, Plant Cell

strig

glands

bracteole

bract

unfertilized ovary

Page 33: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Hops: What we use...

© Nagel et al., 2008, Plant Cell

strig

glands

bracteole

bract

unfertilized ovary

Page 34: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Hops: What we use...

© Nagel et al., 2008, Plant Cell

strig

glands

bracteole

bract

unfertilized ovary

Page 35: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Hops: What we use...

© Nagel et al., 2008, Plant Cell

strig

glands

bracteole

bract

unfertilized ovary

Page 36: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Hops: What we use...

• brewer’s ‘cone’ = botanist’s ‘inflorescence’

– bracts, bracteoles

– it’s all about the glands lupulin resin (lupulone, humulone acids)

– why so many glands?

female only, bitterness

Page 37: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Hops: What we use...

• brewer’s ‘cone’ = botanist’s ‘inflorescence’

– bracts, bracteoles

– it’s all about the glands lupulin resin (lupulone, humulone acids)

– why so many glands?

seed predation deterrence

Page 38: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Hops: What we use...

• brewer’s ‘cone’ = botanist’s ‘inflorescence’

– bracts, bracteoles

– it’s all about the glands lupulin resin (lupulone, humulone acids)

– why so many glands?

seed predation deterrence

flavor/preservative

Page 39: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

© Callens Honey Farm

The End Thank You!

Page 40: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research
Page 41: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Franklin Winslow Yards Brewing Company At age 9, Franklin began home brewing with his father. Years later, he finally figured out why his dad was in the doghouse with Mom after Frank got into the special soda one afternoon. After several years of cancer, HIV, and Anthrax research at the University of Pennsylvania, Frank realized he would always regret it if he did not give professional brewing a shot. In 2004, he took a brewing position at Manayunk Brewery and Restaurant, and later moved on to work as a brewer at Flying Fish in NJ. In 2007, Frank joined the Yards team and has been there ever since, though, he wears his brewers’ boots a lot less and the lab coat a lot more these days as the Director of Quality Assurance.

Page 42: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research
Page 43: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Matt Hall Yards Brewing Company Matt joined Yards in May 2010, after studying brewing science at the Siebel Institute of Technology and a brief stint selling beer for a local wholesaler . He started out on the bottling line hand loading bottles and moved on to the brewhouse as an assistant after only 8 months. After a few months as an assistant there, he moved into a full-time brewer position where he has been working since. Matt’s diploma course at Siebel involved intensive classroom study and hands on brewing experience in Chicago and at the Doemens Academy in Munich Germany. Matt brings this passion for the craft and technical knowledge to Yards every day, and has been a significant asset to the brewing team since he joined them in 2011.

Page 44: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research
Page 45: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Matthew Farber, Ph.D. University of the Sciences Dr. Matthew Farber is the creator and program director of the new Brewing Science Certificate Program at the University of the Sciences where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research focuses on the science of beer with a focus on yeast proteases and their influence on beer quality. He has a B.S. in Biology from Seton Hall University and a Ph.D. in Molecular, Cellular, and Developmental Biology from the University of Pittsburgh. An active member of the American Society of Brewing Chemists, Master Brewers Association, and American Homebrewers Association, Matt aspires to brew better beer through innovative applications of biotechnology.

Page 46: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research
Page 47: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Tim Roberts Yards Brewing Company Tim Roberts is currently the Head Brewer and Production Manager at Yards Brewing Company, and has been brewing professionally for over 15 years. His first exposure to craft beer was at the White Horse on Parson¹s Green in London, where he worked as a cellarman and barman in 1996. And in fact it was there where he first decided to make brewing a career. Tim joined Yards in 2008 where he is responsible for recipe formulation, handling and ordering of raw ingredients, scheduling the brewing and maintenance staff, and logistics for shipping. Also over the past years, Yards has garnered numerous awards for their beers, including World Beer Cup and Great American Beer Festival medals.

Page 48: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research
Page 49: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Fred Schaefer, Ph.D. University of the Sciences Fred Schaefer is an associate professor at the University of the Science in the Department of Chemistry and Biochemistry, a faculty member in the Brewing Science Program and Interim Director of the Forensic Science Program. By training, he is analytical chemist specializing in techniques for characterizing macromolecules. He received his B.A. from Franklin and Marshall College and his Ph.D. from the University of Wisconsin - Madison. He is interested in science activities for the general public; recent efforts include the activity-based “Table Top Science.”

Page 50: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Light Struck Beer

The Photochemistry of Skunked Beer

Fred Schaefer Department of Chemistry & Biochemistry University of the Sciences

Page 51: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Denis De Kekuleire, Interplay of Photochemistry and Beer: How Lightstruck Flavor Is Formed and How It Can Be Prevented, The Spectrum, Center for Photochemical Science, Bowling Green State University, Spring 2001.

Page 52: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research
Page 53: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Denis De Kekuleire, Interplay of Photochemistry and Beer: How Lightstruck Flavor Is Formed and How It Can Be Prevented, The Spectrum, Center for Photochemical Science, Bowling Green State University, Spring 2001.

Page 54: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Iron(III) Oxalate Actinometer

Photochemistry 2 Fe(C2O4)+(aq) → 2 Fe2+(aq) + 2 CO2(g) + C2O4

2-(aq) Color Development Fe2+(aq) + K+(aq) + Fe(CN)6

3-(aq) → KFeIII[FeII(CN)6](s) (Prussian Blue)

B.Z. Shakhashari, Chemical Demonstrations, vol. 5, University of Wisconsin Press, Madison (2011).

Page 55: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research
Page 56: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research

Glass for bottles

Brown Glass

• Iron, sulfur and carbon – Sulfur replaces some oxygen

– High carbon content gives reduced glass

– Color from Fe2+ - Fe3+ charge transfer

– Absorbs wavelength less than 450nm

Green Glass

• Chromium oxide

• Reduced glass gives uv protection.

Page 57: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research
Page 58: Cynthia Skema, Ph.D. University of Pennsylvaniasciencefestivals.org/media/activities_and_programs... · where he is a Teaching Postdoctoral Fellow under Dr. Peter Berget. His research