CURRICULUM VITAE

1. Personal information.

Name: ANTONIO ARTIGUES SERRA.

Office address:

3901 Rainbow Blvd.

1058 HLSIC, MS 3030

Kansas City, KS, 66160

Phone: (913) 588 3487

E-mail: aartigues@kumc.edu

Home Address:

729 W 121 st street

Kansas City, MO 64145

Phone: (816) 941 7403

t_artigues@hotmail.com

2. Education.

Ph.D. in Biochemistry, School of Sciences, University of the Balearic Islands. 1987.

Pharmacy graduate and Master in Pharmacy in Pharmacy, School of Pharmacy, University of Navarra. 1982.

Biology graduate and Master in Biology, School of Sciences, University of Navarra. 1979.

3. Academic appointments or other significant work experience.

2004 – present Research Associate Professor. Director of the Mass Spectrometry Laboratory. Department of Biochemistry and Molecular Biology. School of Medicine. University of Kansas Medical Center. KS

2001 - 2004 Research Assistant Professor and Deputy Director of Research and Development. Mass Spectometry Facility. School of Biological Sciences, University of Missouri-Kansas City.

1996 - 2001 Supervisor of Macromolecular Analysis Core Facilities. School of Biological Sciences. University of Missouri-Kansas City. MO

1992-1996 Research instructor, Department of Molecular Biology and Biochemistry, University of Missouri-Kansas City. MO

1991 Fellow, Neurochemistry Department, School of Medicine, University of Alicante.

1988-1990 postdoctoral researcher, Department of Biochemistry and Molecular Biophysics, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia, USA.

4. Teaching assignments. 2009: Organized the second workshop on Mass spectrometry based

proteomics. Including the participation of Dr. O. Nadeau (KU Medical Center), D. Miller and J. Rogers (thermoFinnigan Institute)

Facilitator in the small group discussions (Huntington Disease) of the Genetics and Neoplasia (core 804)

2008: IGPBS Module 2: Mass spectrometry in proteomics. Application of

mass spectrometry to systems biology (6 impact hours)

Facilitator Small group discussions Huntington Disease (4 impact hour)

2007 IGPBS Module 4: Introduction to proteomics and mass spectrometry (4 impact hours)

2006 Foundations of medicine module 1. Author on Diagnostic applications of proteomics. Group leader on Diagnostic applications of proteomics (4 impact hours) and Mitochondrial DNA and Disease ( 4 impact hours)

2005 IGPBS Module 1: Introduction to proteomics and mass spectrometry.

Facilitator in the Problem based learning of the course BIOC 801 - Medical Biochemistry (Academic Year '06)

2004 IGPBS Module 1: Thermodynamics, protein structure and analysis of reaction/binding kinetics

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1996 Peptide and protein analysis by mass spectrometry (LS MBB 565 structure and function of proteins).

1993-1994: Practical lessons of FT-IR in the course # Chem. 437 (FT-IR), Department of Chemistry, University of Missouri-Kansas City.

5. Awards.

1991: Fellow, Neurochemistry Department, School of Medicine, University of Alicante..

1990: Honorific appointment at the Neurochemistry Department, School of Medicine, University of Alicante.

1987: Cum Laude, Ph. D. Degree. University of the Balearic Islands.

1983: Grant-in-aid of the "Comisión de Obras Sociales de SA NOSTRA".

1977-1978: Fellowship of the University of Navarra. 6. Training and Certificates

2000: Voyager Training Class. PE Biosystems. Foster City, CA.

1996: LCQ Operations Training Course. Finnigan Institute.

1989: Pharmacist specialist in clinical analysis.

1987: Health Diplomate. Pamplona.

7. Membership-Scientific, honorary and Professional Societies.

1995 - to date: Member of the American Chemical Society.

1994 - to date: Member of the Spanish Society of Biochemistry

1993 - to date: Visiting Faculty of the Neurochemistry Department, School of Medicine, University of Alicante.

1993 - to date: Member of the Spanish Society of Biophysics

1992 - to date: Member of the American Association for the Advancement of Science.

8. Technical experience

• Mass Spectrometry/Proteomics • Biological spectroscopy: Circular dichroism, Fluorescence and

Fluorescence polarization, Fourier Infrared Transform Spectroscopy (FT-IR), Stopped-flow

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• Protein purification: ultracentrifugation, Conventional Liquid Chromatography, HPLC, FPLC, Affinity chromatography, Isoelectricfocusing, SDS-PAGE, 2D-PAGE

• Protein mapping and peptide sequencing. • Mass Spectroscopy. Proteomics. • Differential Scanning Calorimetry • Analytical ultracentrifugation • Immunonological techniques: Antibodies production, Western Blot,

ELISA, RIA.

9. Academic Activities

Serving on Mary Ashley Rimmer Thesis Committee 10. Publications

27 Miller D.E.,Prasannan, C.B., Fenton, A.W, and Artigues, A. HDX Finder: automated analysis and datareproting of deuterium/hydrogen exchange mass spectrometry. J. Am. Soc. Mass Spectrom. 2Epub 2011 Nov 15

26 Saito, H-M Yan, A. Artigues, M.T. Villar, A. Farhood and H. Jaeschke (2010): Mechanism of protection by metallothionein against acetaminophen hepatotoxicity. Toxicol Appl Pharmacol. 242, 182-190. PMCID: PMC2789886.

25. Maria T. Villar, Danny E. Miller, Aron W. Fenton and Antonio Artigues (2010)

SAIDE: A Semi-Automated Interface for Hydrogen/Deuterium Exchange Mass Spectrometry Proteomica 6, 63-69

24 Cooper AJ, Krasnikov BF, Niatsetskaya ZV, Pinto JT, Callery PS, Villar MT, Artigues A, Bruschi SA. (2010): Cysteine S-conjugate beta-lyases: important roles in the metabolism of naturally occurring sulfur and selenium-containing compounds, xenobiotics and anticancer agents. Amino Acids. PMCID: PMC2898922.

23. C. Saito, H-M Yang, A. Artigues, M.T. Villar, A. Farhood and H. Jaeshke: Mechanism of protection by metallothionein agains acetaminophen hepatoxicity. Toxicol Appl Pharmacol. 2010 Oct 14. [Epub ahead of print]

22. Boulatnikov, I.G., Nadeau, O.W., Daniels, P.J., Sage, J.M., Jeyasingham, M.D., Villar, M.T., Artigues, A. and Carlson, G.M. (2008). The regulatory beta subunit of phosphorylase kinase interacts with glyceraldehyde-3-phosphate dehydrogenase. Biochemistry 47, 7228-7236.

21. O.W. Nadeau OW, G.J. Wyckoff, J.E. Paschall, A. Artigues, J. Sage, M.T. Villar, and G.M. Carlson: CrossSearch, a user-friendly search engine for detecting chemically cross-linked peptides in conjugated proteins. Mol Cell Proteomics. 2008 Feb 16.

20. H.H-T Hsu, A. Artigues, and M.T. Villar : Induction of calcification by serum depletion in cell culture: a model for focal calcification in aortas related to atherosclerosis. Lipids Health Dis. 2008 Jan 29;7:2.

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19. M. D. Jeyasingham, A. Artigues, O. W. Nadeau, and G.M. Carlson: Co-evolution of the regulation of contraction and energy production in Skeletal Muscle. J Mol Biol. 2008 Mar 28;377(3):623-9. Epub 2008 Jan 5.

19. O.W. Nadeau, D.W. Anderson, Q. Yang, A. Artigues, J.E. Paschall, G.J. Wyckoff, J.M. McClintock and G.M. Carlson: Evidence for the Location of the Allosteric Activation Switch in the Multisubunit phosphorylase kinase complex from mass spectrometric identification of chemically crosslinked peptides.J.Mol. Biol. 2007, 365: 1429-1445

18. A. Artigues, A. Iriarte, and M. Marino Martinez-Carrion: Identification of hsc70 binding sites in mitochondrial aspartate aminotransferase. Archives of Biochemistry and Biophysiscs. 2006, 450(1):30-8.

17. J. A. Oses-Prieto, M.T. Bengoechea-Alonso, A. Arftigues, A. Iriarte, and M. Martinez-Carrion: On the nature of the rate-limiting steps in the refolding of the cofactor-dependent protein aspartate aminotransferase. J. Biol. Chem. 278: 49988-4999 (2003)

16. A. Artigues, A. Iriarte and M. Martinez-Carrion: Binding to Chaperones allows import of a purified mitochondrial precursor into mitochondria. J. Biol. Chem. 277: 25047-25055 (2002).

15. J. P. Gorski, F.-T., L., A. Artigues, L.F. Castagna, and P. Osdoby: New Alternatively Spliced Form of Galectin-3, a Member of α -Galactoside-Binding Animal Lectin Family, Contains Predicted Transmembrane Spanning Domain and Leucine Zipper Motif J. Biol. Chem. 277: 18840-18848 (2002).

14. A. Artigues M. Bengoechea-Alonso, D.L. Crawford, A. Iriarte, and Martinez-Carrion, M: Biological implications of the different Hsp 70 binding properties of mitochondrial and cytosolic aspartate aminotransferase In Biochemistry and Molecular Biology of vitamin B6 and PQQ-dependent proteins. A.Iriarte, H.M. Kagan H.M. and M. Martinez-Carrion (eds.) Birkäuser Verlag Basel/Switzerland pp 111-116 (2000)

13. A. Artigues, D. L. Crawford, A. Iriarte and M. Martinez-Carrion: Divergent Hsc 70-binding properties of mitochondrial and cytosolic aspartate aminotransferase. Implications for their segregation to different cellular compartments. J. Biol. Chem. 273:33130-33134 (1998)

12. F. Donate, A. Artigues, A. Iriarte, and M. Martinez-Carrion: Opposite behavior of two isozymes when refolding in the presence of non-ionic detergents. Protein Science 7:1911-1820 (1998)

11. C. Torella, J. R. Mattingly, Jr., A. Artigues, A. Iriarte, and M. Martinez-Carrion: Insight into the conformation of folding intermediates of a protein trapped by GroEL. J. Biol. Chem. 273:3915-3925 (1998)

10. A. Artigues, A. Iriarte and Martinez-Carrion: Mapping the hsp 70 binding sites on a large mitochondrial precursor protein. In Techniques in Protein Chemistry VIII, D.R. Marshak (editor). Academic Press, pp 481-492 (1997).

9. A. Artigues, A. Iriarte and M. Martinez-Carrion: Refolding intermediates of acid-unfolded mitochondrial aspartate aminotransferase bind to Hsc70. J. Biol. Chem. 272:16852-1861 (1997).

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8. J. P. Gorsky, E. Kremer, J. Ruiz-Perez, G. E. Wise, and A. Artigues: Conformational analyses on soluble surface bound osteopontin. Ann N.Y. Acad Sci USA (1995) 760:12-23.

7. A. Artigues, A. Iriarte and M. Martinez-Carrion: Acid-induced reversible unfolding of mitochondrial aspartate aminotransferase. J. Biol. Chem. 269, 21990-21999 (1994).

6. A. Iriarte, A. Artigues, B. Lain, J. R. Mattingly, Jr. and M. Martinez-Carrion: Cytosolic factors and the twisting path from birth to berth in aspartate aminotransferases. In Biochemistry of Vitamin B6. G. Marino, G. Sannia and F. Bossa (eds.) Birkäuser Verlag Basel/Switzerland, pp 75-80 (1994).

5. A. Artigues, H. Farrant and V. Schirch: Role of the amino terminal region of serine hydroxymethyltransferase in determining its in vivo rate of turnover. J. Biol. Chem. 268, 13784-13790 (1993).

4. A. Artigues, A. Birkett and S. Verne: Evidence for the in vivo deamidation and isomerization of an asparaginyl residue in cytosolic serine hydroxymethyltransferase. J. Biol. Chem. 265, 4853-4858 (1990).

3. A. Artigues, M. T. Villar, A. Fernandez, J. A. Ferragut, and J. M. Gonzlez-Ros: Cholesterol modulates structural features of the nicotinic acetylcholine receptor in model reconstituted vesicles. Biochem. Biochim. Acta 985, 325-330 (1989).

2. M. T. Villar, A. Artigues, J. A. Ferragut and J. M. González-Ros: Phospholipase A2 hydrolysis products as membrane specific perturbants to probe structural features of nicotinic acetylcholine receptor. Biochem. Biochim. Acta. 938, 35-43 (1988).

1. A. Artigues, M. T. Villar, J. A. Ferragut and J. M. González-Ros: Thermal perturbation studies of membrane-bound acetylcholine receptor from Torpedo: Effects of cholinergic ligands and membrane perturbants. Arch. Biochem. Biopsy. 258, 33-41 (1987).

9.2 Invited conferences/lectures

2009: Emerging Trends presentation: Proteomics: Keys to Treatment, Cure & Beyond. School of Nursing, KU Medical Center.

2008: Protein structure and function by mass spectrometry. Centre de Regulacio Genomica. Barcelona, Spain

2007: Teaching workshop on protein mass spectrometry: Mass spectrometry studies on protein folding. University of Kansas. Lawrence, Kansas.

2006: Aplicaciones de la Espectrometria de Masas al Estudio de las Interacciones Proteina-Proteina, University of Alicante, Spain.

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2005: “Mass Spectrometry: a tool for protein dynamics”. Facultat de Veterinaria, Universitat Autonoma de Barcelona, Spain. May 2, 2005

2001: Regional Biophysics Collaboration: “Mass Spectrometry and Proteomics”. School of Biological Sciences, University of Missouri-Kansas City. February 22, 2001

1999: 10th international symposium on vitamin B6 and carbonyl catalysis and 4th meeting on PQQ and quinoproteins: “Divergent HSP70 Binding Properties of Two Aspartate Aminotransferase Isozymes: Biological Implications.” Santa Fe, New Mexico, USA. October 31-November 5, 1999.

9.3 Communications (posters)

Hydrogen-deuterium exchange mass spectrometry to identify allosterically relevant changes in proteins. Prassannan, C.B., Villar, M.T., Artigues, A., and Fenton A.W. 10th International Symposium on Mass Spectrometry in the Health and Life Sciences: Molecular and Cellular Proteomics. San Francisco, CA August 21-25 (2011).

The Structure of early folding intermediates of mitochondrila aspartate aminotransferase and its reduced form. Villar, M.T. and Artigues, A. 10th International Symposium on Mass Spectrometry in the Health and Life Sciences: Molecular and Cellular Proteomics. San Francisco, CA August 21-25 (2011).

Identification of regions of rabbit muccle pyruvate kinase important for allosteric regulation by phenylalanine detected by deuterium-hydrogen exchange mass spectrometry. Prassannan, C.B., Villar, M.T., Artigues, A., and Fenton A.W. 23rd ASMS Sanibel Conference. From fragmentation mechanisms to sequencing: Tandem Mass Spectrometry Based Peptide and Protein Identification. Sanibel, FL January 21-24 2012.

H. Changotra, A. Artigues and L. Hutt-Fletcher. The cellular protein p32 interacts with the cytoplasmic tail of EBV gM and is required for efficient egress of virions from the cell. 35th International Herpesvirus Workshop. Salt Lake City, Utah July 24-29 2010. .

L. Novikova, L. Stehno-Bittel, A. Artigues, M.T. Villar, S. J. Williams, H.-H. Huang, K. Kover and I. V. Smirnova. Pancreatic islet transplantation to treat diabetes – defining molecular tools to select suitable islets. Missouri Regional Life Sciences Summit, "Animal to Human Health Collaborations – Regional Partnerships for Innovation." Kansas City, MO March 8th, 2010.

M.T. Villar, D. E. Miller, A.W. Fenton and A. Artigues. Deuterium exchange mass spectrometry using a semiautomatic interface for data acquisition and automatic data analysis and reporting. ASMS 57th annual Conference on Mass Spectrometry and Allied Topics annual Conference. Philadelphia, PA, May 31- June 4, 2009.

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M M. T. Villar and A. Artigues: Folding dependence of a Mitochondrial Precursor Protein on elements of its primary sequence Protein Society meeting, San Diego, 2008.

D. E. Miller, A. Artigues and A. W. Fenton. H/DX-MS detection of changes in protein dynamics of pyruvate kinase elicited by binding of allosteric vs. nonallosteric effector analogs (oral presentation). 22nd Annual Gibbs Conference on Biothermodynamics October, 2008

Y Dong, PhD1, W Hou, PhD1, A Artigues, PhD2, M Villar, PhD2 and C P Weiner, MD, MBA1. Fetal adaptation to chronic hypoxemia (hpx) alters the Proteome of the fetal brain in a dose dependent fashion. Sgi 2008 annual scientific meeting.

M.T. Villar and A. Artigues. Refolding of cytosolic aspartate aminotransferase analyzed by hydrogen exchange and mass spectrometry. 21th Symposium of the Portein Society, July 21-25, 2007

M. T. Villar, Z. V. Niatsetskaya, A. J. L. Cooper, and A. Artigues Inactivation of rat liver mitochondrial aspartate aminotransferase by a halogenated cysteine conjugate 8th on Mass Spectrometry in the Health and Life Sciences. San Francisco, 2007

Owen Nadeau, Justin Paschall, Gerald Wyckoff, Antonio Artigues, Jessica Sage, Maria Teresa Villar, Gerald Carlson Detection of Intrasubunit Interactions in the Regulatory Beta Subunit in the exadecameric Phosphorylase Kinase Complex Using a Data-based Search Engine to Detect Chemically Cross-linked Peptides by Mass Spectrometry 21th Symposium of the Protein Society, July 21-25, 2007

O. W. Nadeau, A. Artigues, J.E. Paschall, G.J. Wyckoff and G.M. Carlson, “ Mapping a Phosphorylation-dependent Subunit Communication Network in the Hexadecameric Phosphorylase kinase Complex Using a Data-based Search engine to Detect Chemically Cross-linked Peptides by Mass Spectrometry.” Protein Science 15, Abs. # 457, 224, 2006

M. Jeyasingham, A. Artigues, G. Carlson: A Phosphoproteomic Approach to Identifying Kinases that use Phosphoenolpyruvate as a Phosphoryl Donor. 20th Symposium of the Portein Society, August 5-9, 2006

A. Artigues, J. A. Oses-Prieto, A. Iriarte, and M. Martinez-Carrion: Probing the molten globule structure of a globular protein on a Ion Clyclotron-Fourrier Transform Mass Spectrometer in combination with Hydrogen Exchange. 6th European Symposium of the Protein Society. Barcelona, Spain, April 30- May 4, 2005

Owen W. Nadeau, David Anderson, Qing Yang, Antonio Artigues and Gerald M. Carlson: The N-terminus of the regulatory β subunit of phosphorylase kinase mediates self-association of this subunit in the activated holoenzyme complex. 18th Symposium of the Protein Society, San Diego, CA: August 14-18, 2004.

J. A. Oses-Prieto, A. Artigues, A. Iriarte, M. Martinez-Carrion: Refolding of Aspartate Aminotransferase Analyzed by Hydrogen Exchange and Mass Spectrometry. 6th symposium on Mass Spectrometry in the Health and Life Sciences. San Francisco, 2003

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A. Artigues, A. Iriarte, and M. Martinez-Carrion: Binding to Chaperones and Import of a Purified precursor Protein into Mitochondria. 16th symposium of the Protein Society. San Diego, CA. August 17-21, 2002..

M. Jeyasingham, A. Artigues, N. Nadeau, and G. Carlson: Zero-Lengh Crosslinking of the Intrinsic Catalytic Gamma Subunit of Phosphorylase Kinase with Either its Regulatory Alpha or delta Subunits Is Oppositley Affected by Ca2+. 16th symposium of the Protein Society. August 17-21, 2002. San Diego, CA.

A. Artigues, A. Iriarte, and M. Martinez-Carrion:Import of a Mitochondrial Precursor Complexed to Hsc70 Into Isolated mitochondria. Fifth International Symposium on Mass Spectrometry in the Health and Life Sciences: Molecular and Cellular Proteomics. August 26-30, 2001 San Francisco, CA.

J. Oses-Prieto, A. Artigues, A. Iriarte and M. Martinez-Carrion: The presence of covalently attached cofactor can bias the interpretation of protein refolding kinetics.

FASEB, Experimetnal Biology 2001 Meetting. Orlando, Forida. USA (2001).

C. Torella, J. R. Mattingly, Jr., A. Artigues, A. Iriarte, and M. Martinez-Carrion: Insight into the conformation of folding intermediates of a protein trapped by GroEL. Forty-second Annual Meeting of the Biophysical Society. Kansas City, Missouri, USA (1998).

A. Artigues, A. Iriarte and M. Martinez-Carrion: Mapping the binding sites of hsp70 into a large mitochondrial precursor protein. Protein Society. San Joss, California, USA (1996).

A. Artigues, A. Iriarte and M. Martinez-Carrion: hsp70 can interact in vitro with an unfolded mitochondrial precursor protein but not with its cytosolic isozyme. Protein Society. Boston, MD, USA (1995).

A. Artigues, A. Iriarte and M. Martinez-Carrion: In vitro refolding of Mitochondrial Aspartate Aminotransferase. ASBMB/ACS Joint meeting. San Diego, California, USA. (1993).

A. Artigues, A. Fernandez and J. M. González-Ros: Purificación de subunidades del Receptor Nicotínico de Acetilcolina mediante electroelución contínua. Congreso de Neurociencias. Alicante. (1991).

A. Artigues and V. Schirch: Role of the aminoterminal region of serine hydroxymethyltransferase in determining its in vivo rate of degradation. FASEB Summer Research Conference on Folic acid, B12 and One Carbon Metabolism. Xaxon, Vermont, USA (1990).

A. Artigues and V. Schirch: Role of the aminoterminal region of serine hydroxymethyltransferase in determining its in vivo rate of degradation. ASBMB/AAI Joint meeting, New Orleans, USA (1990).

J. M. González-Ros, M. T. Villar, A. Artigues y J.A. Ferragut: Dependencia de parametros estructurales del receptor nicotínico de acetilcolina con su entorno

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lipídico. II Congreso de la Sociedad Española de Neurociencias. Barcelona, España (1987).

M. T. Villar, A. Artigues, J. M. González-Ros: Modificación de las propiedades estructurales del receptor nicotínico de acetilcolina por la Fosfolipasa A2. XIII Congreso Nacional de la Sociedad Espalñola de Bioquímica. Zaragoza, España (1986).

J. M. González-Ros, A. Artigues, M. T. Villar y J. A. Ferragut: Heat inactivation studies of membrane-bound AcChR from Torpedo: Effect of cholinergic ligands and membrane perturbants. 3td Symposium Handling of environmental and biological samples in chromatography. Palma de Mallorca, España (1986).

M. T. Villar, A. Artigues, J. Ferragut and J. M. González-Ros: Modificación de las propiedades del receptor nicotínico de acetilcolina por proteínas periféricas de membrana. XII Congreso Nacional de la Sociedad Española de Bioquímica. Valencia, España (1985)

Ongoing Research Support R01DE018713 (J. Wang, PI)

Bone sialoprotein in osteogenesis and bone regeneration

Goal: to study the mechanism of bone sialprotein-mediated osteogenesis and bone regeneration

Role: Collaborator

2R56DK032953-26 (G.M. Carlson, PI) Subunit Interactions of Phosphorylase Kinase

Goal: To study the structure, mechanism of action and regulation of phosphorylase kinase

Role: Collaborator

1 R01 DK078076-01A1 (A.W. Fenton, PI)

Dissecting Allostery in Pyruvate Kinase

Goal: To study the allsoteric regulation and dynamics of Pyruvate kinase

Role: Collaborator

Completed Research Support

NIH - R21 DK072393-02 (G.M. Carlson, PI)

9/01/05 - 6/30/08

Title: Proteomics to screen for a protein kinase that uses PEP

Role: Collaborator.

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NIH - 2 R56 HL067336-05A (K.R. Peterson, PI)

7/15/08 - 7/14/09

Title: Locus linked regulatory motifs of globin gene switching

Role: Other Significant Contributor

NIH - 5 P20 RR024214-02 (D.R. Abrahamson, PI; L. Christenson, subproject leader)

9/27/07 - 6/30/12

Title: Molecular Regulation of Cell Development and Differentiation

Role: Collaborator

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CURRICULUM VITAE

James P. Calvet

Address and Phone

Department of Biochemistry and Molecular Biology

6001 Wahl Hall East, Kidney Institute

University of Kansas Medical Center, MS3018

Kansas City, Kansas 66160

Tel: (913) 588-7424 Fax: (913) 588-9251

Email: jcalvet@kumc.edu

Date and Place of Birth: November 6, 1945; Washington, D.C.

Family Status: Married; three daughters

Education

University of Connecticut Ph.D. Genetics and 1975

Storrs, Connecticut Cell Biology

State University College M.A. Biology 1970

Plattsburgh, New York

Franklin Pierce College B.A. Biology 1968

Rindge, New Hampshire

Professional Experience

2012-present Deputy Director, Kidney Institute, University of Kansas Medical Center

2009-present Co-Leader, Cancer Biology Program, University of Kansas Cancer Center

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2009-2011 Interim Director, Kidney Institute, University of Kansas Medical Center

2005-present Director, Kansas Interdisciplinary Center for PKD Research, University of Kansas Medical Center

1994-present Professor (tenured), Department of Biochemistry and Molecular Biology, School of Medicine, University of Kansas Medical Center

1988-1994 Associate Professor (tenured), Department of Biochemistry and Molecular Biology, School of Medicine, University of Kansas Medical Center

1981-1988 Assistant Professor, Department of Biochemistry, School of Medicine, University of Kansas Medical Center

1978-1981 Senior Research Associate (with Dr. Thoru Pederson), Department of Cell Biology, Worcester Foundation for Experimental Biology

1975-1978 Postdoctoral Fellow in Cell Biology (with Dr. Thoru Pederson), American Cancer Society Postdoctoral Fellow (1976-1977), Worcester Foundation for Experimental Biology

Honors and Awards

Student Voice Award for Excellence in Teaching Medical Biochemistry, 1996-97.

Investigator Research Award, School of Medicine, KUMC 2004.

Chancellor’s Distinguished Teaching Award for Excellence in Teaching, University of Kansas, 2007

Chancellor’s Club Research Award, University of Kansas, 2009

Lillian Jean Kaplan International Prize for Advancement in the Understanding of Polycystic Kidney Disease, awarded jointly by the International Society of Nephrology and PKD Foundation, 2011.

John C. Davis Memorial Lecture, University of Kansas, 2012

Professional Societies

American Society of Biochemistry and Molecular Biology

American Society for Cell Biology

American Society of Nephrology

International Society of Nephrology

American Association for the Advancement of Science

Research Interests

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Molecular biology and molecular genetics of polycystic kidney disease; regulation of cell proliferation, signal transduction, transcriptional mechanisms, gene expression in normal and abnormal kidney development; cellular, molecular, and developmental biology; human genetics and cancer.

Publications (peer reviewed)

Laufer, H. and Calvet, J.P. (1972) Hormonal effects on chromosomal puffs and insect development. Gen. and Comp. Endocr. Suppl. 3, 137-148.

Calvet, J.P. and Clark, R.M. (1974) Immersion refractometric analysis of cultured Hyalophora cecropia hemocytes. Protoplasma 80, 29-40.

Calvet, J.P. and Pederson, T. (1977) Secondary structure of heterogeneous nuclear RNA: Two classes of double-stranded RNA in native ribonucleoprotein. Proc. Natl. Acad. Sci. U.S.A. 74, 3705-3709.

Calvet, J.P. and Pederson, T. (1978) Nucleoprotein organization of inverted repeat DNA transcripts in heterogeneous nuclear RNA-ribonucleoprotein particles from HeLa cells. J. Mol. Biol. 122, 361-378.

Calvet, J.P. and Pederson, T. (1979) Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by cross-linking with the psoralen derivative aminomethyltrioxsalen. Proc. Natl. Acad. Sci. U.S.A. 76, 755-759.

Calvet, J.P. and Pederson, T. (1979) Photochemical cross-linking of secondary structure in HeLa cell heterogeneous nuclear RNA in situ. Nuc. Acids Res. 6, 1993-2001.

Calvet, J.P. and Pederson, T. (1981) Base-pairing interactions between small nuclear RNAs and nuclear RNA precursors as revealed by psoralen cross-linking in vivo. Cell 26, 363-370.

Calvet, J.P., Meyer, L.M. and Pederson, T. (1982) Small nuclear RNA U2 is base-paired to heterogeneous nuclear RNA. Science 217, 456-458.

Roberts, M.P., Falvey, E., Calvet, J.P. and Pederson, T. (1983) A sequence related to 4.5S RNA and the B1 family of repeated DNA in the 5' flanking region of the mouse β-globin gene. J. Mol. Biol. 169, 975-982.

Kunkel, G., Maser, R.L., Calvet, J.P. and Pederson, T. (1986) U6 small nuclear RNA is transcribed by RNA polymerase III. Proc. Natl. Acad. Sci. U.S.A. 83, 8575-8579.

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Smardo, F.L., Jr. and Calvet, J.P. (1987) Human glutamate tRNA forms stable hybrids in vitro with 28S ribosomal RNA. Nuc. Acids Res. 15, 661-681.

Smardo, F.L., Jr. and Calvet, J.P. (1987) Sequence analysis of the glutamate tRNA family: evidence for pseudogenes. Gene 57, 213-220.

Andrews, G.K., Harding, M.A., Calvet, J.P. and Adamson, E.D. (1987) The heat shock response in HeLa cells is accompanied by elevated expression of the c-fos proto-oncogene. Mol. Cell. Biol. 7, 3452-3458.

Calvet, J.P. and Myers, J.A. (1987) In vivo secondary structure analysis of the small nuclear RNA U1 using psoralen cross-linking. J. Mol. Biol. 197, 543-553.

Cowley, B.D., Jr., Smardo, F.L., Jr., Grantham, J.J. and Calvet, J.P. (1987) Elevated c-myc proto-oncogene expression in autosomal recessive polycystic kidney disease. Proc. Natl. Acad. Sci. U.S.A. 84, 8394-8398.

Gattone, V.H., Calvet, J.P., Cowley, B.D., Jr., Evan, A.P., Shaver, T.S., Helmstadter, K. and Grantham, J.J. (1988) Autosomal recessive polycystic kidney disease in a murine model: a gross and microscopic description. Lab. Invest. 59, 231-238.

Cowley, B.D., Jr., Chadwick, L.J., Grantham, J.J. and Calvet, J.P. (1989) Sequential proto-oncogene expression in regenerating kidney following acute renal injury. J. Biol. Chem. 264, 8389-8393.

Maser, R.L. and Calvet, J.P. (1989) U3 small nuclear RNA can be psoralen cross-linked in vivo to the 5' external transcribed spacer of pre-ribosomal RNA. Proc. Natl. Acad. Sci. U.S.A. 86, 6523-6527.

Mangoo-Karim, R., Uchic, M.E., Grant, M., Shumate, W.A., Calvet, J.P., Park, C.H. and Grantham, J.J. (1989) Renal epithelial fluid secretion and cyst growth: The role of cyclic AMP. FASEB J. 3, 2629-2632.

Grantham, J.J., Mangoo-Karim, R., Uchic, M.E., Grant, M., Shumate, W.A., Park, C.H. and Calvet, J.P. (1989) Net fluid secretion by mammalian renal epithelial cells: stimulation by cAMP in polarized cultures derived from established renal cells and from normal and polycystic kidneys. Trans. Assoc. Am. Physicians 102, 158-162.

Gattone, V.H., Andrews, G.K., Niu Fu-wen, Chadwick, L.J., Klein, R.M. and Calvet, J.P. (1990) Defective epidermal growth factor gene expression in mice with polycystic kidney disease. Dev. Biol. 138, 225-230.

15

Takahashi, H., Calvet, J.P., Dittemore-Hoover, D., Yoshida, K., Grantham, J.J. and Gattone, V.H. (1991) A hereditary model of slowly progressive polycystic kidney disease in the mouse. J. Am. Soc. Nephrol. 1, 980-989.

Cowley, Jr., B.D., Chadwick, L.J., Grantham, J.J. and Calvet, J.P. (1991) Elevated proto-oncogene expression in polycystic kidneys of the C57BL/6J (cpk) mouse. J. Am. Soc. Nephrol. 1, 1048-1053.

Gattone, V.H. and Calvet, J.P. (1991) Murine infantile polycystic kidney disease: a role for reduced renal epidermal growth factor. Am. J. Kid. Dis. 17, 606-607.

Harding, M.A., Chadwick, L.J., Gattone, V.H. and Calvet, J.P. (1991) The SGP-2 gene is developmentally regulated in the mouse kidney and abnormally expressed in collecting duct cysts in polycystic kidney disease. Dev. Biol. 146, 483-490.

Harding, M.A., Gattone, V.H., Grantham, J.J. and Calvet, J.P. (1992) Localization of overexpressed c-myc mRNA in polycystic kidneys of the cpk mouse. Kidney Int. 41, 317-325.

Rankin, C.A., Grantham, J.J. and Calvet, J.P. (1992) c-fos expression is hypersensitive to serum-stimulation in cultured cystic kidney cells from the C57BL/6J-cpk mouse. J. Cell. Physiol. 152, 578-586.

Birkenmeier, E.H., Letts, V.A., Frankel, W.N., Magenheimer, B.S. and Calvet, J.P. (1993) Sulfated glycoprotein-2 (Sgp-2) maps to mouse chromosome 14. Mammalian Gen. 4, 131-132.

Cowley, Jr., B.D., Gudapaty, S., Kraybill, A.L., Harding, M.A., Calvet, J.P. and Gattone, V.H. (1993) Autosomal dominant polycystic kidney disease in the rat. Kidney Int. 43, 522-534.

Lowden, D.A., Lindemann, G.W., Merlino, G., Barash, B.D., Calvet, J.P. and Gattone, V.H. (1994) Renal cysts in transgenic mice expressing transforming growth factor-α. J. Lab. Clin. Med. 124, 386-394.

Maser, R.L., Magenheimer, B.S. and Calvet, J.P. (1994) Mouse plasma glutathione peroxidase: cDNA sequence analysis and renal proximal tubule expression and secretion. J. Biol. Chem. 269, 27066-27073.

Calvet, J.P. and Chadwick, L.J. (1994) Primary and secondary genetic responses following folic acid-induced acute renal injury in the mouse. J. Am. Soc. Nephrol. 5, 1324-1332.

Maser, R.L. and Calvet, J.P. (1995) Analysis of differential gene expression in the kidney by differential cDNA screening, subtractive cloning, and mRNA differential display. Sem. Nephrol. 15, 29-42.

Rankin, C.A., Ziemer, D.M., Maser, R.L., Foo, I. and Calvet, J.P. (1996) Growth characteristics of cells cultured from two murine models of polycystic kidney disease. In Vitro Cell. Dev. Biol. 32, 100-106.

Hou, X., Maser, R.L., Magenheimer, B.S. and Calvet, J.P. (1996) A mouse kidney- and liver-expressed cDNA having homology with a prokaryotic parathion hydrolase (phosphotriesterase) gene: abnormal expression in injured and polycystic kidneys. Gene 168, 157-163.

Gattone, V.H., Kuenstler, K.A., Lindemann, G.W., Lu, X., Cowley, B.D., Rankin, C.A. and Calvet, J.P. (1996) Renal expression of a transforming growth factor alpha transgene accelerates the progression of inherited, slowly progressive polycystic kidney disease in the mouse. J. Lab. Clin. Med. 127, 214-222.

Davidow, C.J., Maser, R.M., Rome, L.A., Calvet, J.P. and Grantham, J.J. (1996) The cystic fibrosis transmembrane conductance regulator (CFTR) mediates transepithelial fluid secretion by human autosomal dominant polycystic kidney disease (ADPKD) epithelium in vitro. Kidney Int. 50, 208-218.

Rankin, C.A., Suzuki, K., Itoh, Y., Ziemer, D.M., Grantham, J.J., Calvet, J.P. and Nagase, H. (1996) Matrix metalloproteinases and TIMPS in cultured C57BL/6J-cpk kidney tubules. Kidney Int. 50, 835-844.

Parnell, S.C., Magenheimer, B.S., Maser, R.L., Rankin, C.A., Smine, A., Okamoto, T. and Calvet, J.P. (1998) The polycystic kidney disease-1 protein, polycystin-1, binds and activates heterotrimeric G-proteins in vitro. Biochem. Biophys. Res. Comm. 251, 625-631.

Witzgall, R., Obermuller, N., Bolitz, U., Calvet, J.P., Cowley, B.D., Walker, C., Kriz, W., Gretz, N. and Bonventre, J.V. (1998) Kid-1 expression is high in differentiated renal proximal tubule cells and suppressed in cyst epithelia. Am. J. Physiol. 275, F928-F937.

Rankin, C.A., Itoh, Y., Tian, C., Ziemer, D.M., Calvet, J.P. and Gattone, V.H. (1999) Matrix metalloproteinase 2 in a murine model of infantile polycystic kidney disease. J. Am. Soc. Nephrol. 10, 210-217.

Parnell, S.C., Magenheimer, B.S., Maser, R.L. and Calvet, J.P. (1999) Identification of the major site of in vitro PKA phosphorylation in the polycystin-1 C-terminal cytosolic domain. Biochem. Biophys. Res. Comm. 259, 539-543.

Ricker, J.L., Gattone, V.H., Calvet, J.P. and Rankin, C.A. (2000) Development of autosomal recessive polycystic kidney disease in Balb-c cpk/cpk mice. J. Am. Soc. Nephrol. 11, 1837-1847.

Sutters, M., Yamaguchi, T., Maser, R.L., Magenheimer, B.S., St. John, P.L., Abrahamson, D.R., Grantham, J.J. and Calvet, J.P. (2001) Polycystin-1 transforms the cAMP growth-responsive phenotype of M-1 cells. Kidney Int. 60, 484-494.

Maser, R.L., Vassmer, D., Magenheimer, B.S. and Calvet, J.P. (2002) Oxidant stress and reduced antioxidant enzyme protection in polycystic kidney disease. J. Am. Soc. Nephrol. 13, 991-999.

Ledford, A.W., Brantley, J.G., Kemeny, G., Foreman, T.L., Quaggin, S.E., Igarashi, P., Oberhaus, S.M., Rodova, M., Calvet, J.P. and Vanden Heuvel, G.B. (2002) Deregulated expression of the homeobox

gene Cux-1 in transgenic mice results in down regulation of p27kip1 expression during nephrogenesis, glomerular abnormalities, and multiorgan hyperplasia. Dev. Biol. 245, 157-171.

Parnell, S.C., Magenheimer, B.S., Maser, R.L., Zien, C.A., Frischauf, A.-M. and Calvet, J.P. (2002) Polycystin-1 mediated activation of c-Jun-N-terminal kinase and AP-1 is regulated by heterotrimeric G proteins. J. Biol. Chem. 277, 19566-19572.

Wolf, D., Rodova, M., Miska, E.A., Calvet, J.P. and Kouzarides, T. (2002) Acetylation of -catenin by CREB-binding protein (CBP). J. Biol. Chem. 277, 25562-25567.

Rodova, M., Islam, M.R., Maser, R.L. and Calvet, J.P. (2002) The polycystic kidney disease-1 promoter is a target of the -catenin/T-cell factor pathway. J. Biol. Chem. 277, 29577-29583.

Islam, M.R., Rodova, M. and Calvet, J.P. (2002) A fast and efficient method of DNA fragment isolation from agarose gels without using commercial kits. Amer. Biotech. Lab. 20, 18.

Rodova, M., Islam, M.R., Peterson, K.R. and Calvet, J.P. (2003) Remarkable sequence conservation of the last intron in the PKD1 gene. Molec. Biol. Evol. 20, 1669-1674.

Le, N.H., Van Der Bent, P., Huls, G., Van De Wetering, M., Loghman-Adham, M., Ong, A.C., Calvet, J.P., Clevers, H., Breuning, M.H., Van Dam, H. and Peters, D.J. (2004) Aberrant polycystin-1 expression results in modification of AP-1 activity, while Wnt signaling remains unaffected. J. Biol. Chem. 279, 27472-27481.

Yamaguchi, T., Wallace, D.P., Magenheimer, B.S., Hempson, S.J., Grantham, J.J., and Calvet, J.P. (2004) Calcium restriction allows cAMP activation of the B-Raf/ERK pathway, switching cells to a cAMP-dependent growth-stimulated phenotype. J. Biol. Chem. 279, 40419-40430.

Puri, S., Magenheimer, B.S., Maser, R.L., Ryan, E., Zien, C.A., Walker, D.D., Wallace, D.P., Hempson, S.J., and Calvet, J.P. (2004) Polycystin-1 activates the calcineurin/NFAT (nuclear factor of activated T-cells) signaling pathway. J. Biol. Chem. 279, 55455-55464.

Puri, S., Rodova, M., Islam, M.R., Magenheimer, B.S., Maser, R.L., and Calvet, J.P. (2006) Ets factors regulate the polycystic kidney disease-1 (PKD1) promoter. Biochem. Biophys. Res. Comm. 342, 1005-1013.

Xiao, Z., Zhang, S., Malios, J., Zhou, G., Magenheimer, B.S., Guo, D., Dallas, S.L., Maser, R., Calvet, J.P., Bonewald, L., Quarles, L.D. (2006) Cilia-like structures and polycystin-1 in osteoblasts/osteocytes and associated abnormalities in skeletogenesis and Runx2 expression. J. Biol. Chem. 281, 30884-30895.

Van Bodegom, D., Saifudeen, Z., Dipp, S., Puri, S., Magenheimer, B.S., Calvet, J.P., and El-Dahr, S.S. (2006) The polycystic kidney disease-1 gene is a target for p53-mediated transcriptional repression. J. Biol. Chem. 281, 31234-31244.

Magenheimer, B.S., St. John, P.L., Isom, K.S., Abrahamson, D.R., De Lisle, R.C., Wallace, D.P., Maser, R.L., Grantham, J.J., and Calvet, J.P. (2006) Early embryonic renal tubules of wild-type and PKD kidneys respond to cAMP stimulation with CFTR/NKCC1-dependent cystic dilation. J. Am. Soc. Nephrol. 17, 3424-3437.

Nagao, S., Nishii, K., Yoshihara, D., Kurahashi, H., Nagaoka, K., Yamashita, T., Takahashi, H., Yamaguchi, T., Calvet, J.P., and Wallace, D.P. (2008) Calcium channel inhibition with verapamil accelerates polycystic kidney disease progression in the Cy/+ rat. Kidney Int. 73, 269-277.

Li, X., Magenheimer, B.S., Xia, S., Johnson, T., Wallace, D.P., Calvet, J.P., and Li, R. (2008) A tumor necrosis factor-alpha-mediated pathway promoting autosomal dominant polycystic kidney disease. Nature Medicine 14, 863-868.

Islam, M.R., Puri, S., Rodova, M., Magenheimer, B.S., Maser, R.L., and Calvet, J.P. (2008) Retinoic acid-dependent activation of the polycystic kidney disease-1 (PKD1) promoter. Am. J. Physiol. Renal Physiol. 295, F1845-1854.

Katayama, H., McGill, M., Kearns, A., Brzozowski, M., Degner, N., Harnett, B., Kornilayev, B., Matković-Čalogović, D., Holyoak, T., Calvet, J.P., Gogol, E.P., Seed, J., Fisher, M.T. (2009) Strategies for folding of affinity tagged proteins using GroEL and osmolytes. J. Struct. Funct. Genomics. 10(1), 57-66.

Nagao, S., Morita, M., Kugita, M., Yoshihara, D., Yamaguchi, T., Kurahashi, H., Calvet, J.P., and Wallace, D.P. (2010) Polycystic kidney disease in Han:SPRD Cy rats is associated with elevated expression and mislocalization of SamCystin. Am. J. Physiol. Renal Physiol. 299(5), F1078-1086.

Yamaguchi, T., Reif, G.A., Calvet, J.P., and Wallace, D.P. (2010) Sorafenib inhibits cAMP-dependent ERK activation, cell proliferation, and in vitro cyst growth of human ADPKD cyst epithelial cells. Am. J. Physiol. Renal Physiol. 299(5), F944-951.

Islam, M.R., Jimenez, T., Pelham, C., Rodova, M., Puri, S., Magenheimer, B.S., Maser, R.L., Widmann, C., and Calvet, J.P. (2010) MAP/ERK kinase kinase 1 (MEKK1) mediates transcriptional repression by interacting with polycystic kidney disease-1 (PKD1) promoter-bound p53 tumor suppressor protein. J. Biol. Chem. 285, 38818-38831.

Kugita, M., Nishii, K., Morita, M., Yoshihara, D., Kowa-Sugiyama, H., Yamada, K., Yamaguchi, T., Wallace, D.P., Calvet, J.P., Kurahashi, H., and Nagao, S. (2011) Global gene expression profiling in early-stage polycystic kidney disease in the Han:SPRD Cy rat identifies a role for RXR signaling. Am. J. Physiol. Renal Physiol. 300 (1), F177-F188.

Yoshihara, D., Kurahashi, H., Morita, M., Kugita, M., Hiki, Y., Aukema, H.M., Yamaguchi, T., Calvet, J.P., Wallace, D.P., and Nagao, S. (2011) PPAR-γ agonist ameliorates kidney and liver disease in an orthologous rat model of human autosomal recessive polycystic kidney disease. Am. J. Physiol. Renal Physiol. 300 (2), F465-474.

Fan, L.X., Li, X., Magenheimer, B.S., Calvet, J.P., Li, X. (2012) Inhibition of histone deacetylases targets the transcription regulator Id2 to attenuate cystic epithelial cell proliferation. Kidney Int. 81 (1), 76-85.

Parnell, S.C., Puri, S., Wallace, D.P., and Calvet, J.P. (2012) Protein phosphatase-1 in te ra cts with and dephosphorylates polycystin-1. PLoS ONE, 7 (6) e36798 (11 pages).

Yoshihara, D., Kugita, M., Yamaguchi,T., Aukema, H.M., Kurahashi, H., Morita, M., Hiki, Y., Calvet, J.P., Wallace, D.P., Toyohara, T., Abe, T., Nagao, S. (2012) Global Gene Expression Profiling in PPAR-γ Agonist-Treated Kidneys in an Orthologous Rat Model of Human Autosomal Recessive Polycystic Kidney Disease. PPAR Res. 2012, 695898 (10 pages).

Jansson, K., Nguyen, A., Magenheimer, B.S., Reif, G.A., Reddy, A.L., Bello-Reuss, E., Wallace, D.P., Calvet, J.P., and Blanco, G. (2012) Endogenous concentrations of ouabain act as a cofactor to stimulate fluid secretion and cyst growth of in vitro ADPKD models via cAMP and EGFR-Src-MEK pathways. Am. J. Physiol. Renal Physiol. 303 (7), F982-990.

Chapters, Reviews (invited)

Calvet, J.P. (1990) Molecular Biology, Gene Expression, and Medicine. In Topics in Renal Medicine: Inheritance of Kidney and Urinary Tract Diseases (Spitzer, A. and Avner, E.D., eds.) Kluwer, Boston, pp. 3-51. (book chapter).

Calvet, J.P. and Cowley, B.D., Jr. (1990) Proto-Oncogene Expression in Autosomal Recessive Polycystic Kidney Disease. In Advances in the Pathogenesis of Polycystic Kidney Disease (Carone, F.A. and Dobbie, J.W., eds.) TP&T, Chicago, pp. 33-36. (review).

Calvet, J.P. (1991) Molecular approaches for analyzing differential gene expression: differential cDNA library construction and screening. Pediatr. Nephrol. 5, 751-757. (basic science review).

Calvet, J.P. (1993) Polycystic kidney disease: primary extracellular matrix abnormality or defective cellular differentiation? Kidney Int. 43, 101-108. (review and opinion).

Calvet, J.P. (1993) Proto-Oncogenes and Abnormalities of Differentiation in PKD. In Proceedings of the Fifth International Workshop on Polycystic Kidney Disease (Gabow, P.A. and Grantham, J.J., eds.) Polycystic Kidney Research Foundation, Kansas City, pp. 108-113. (review and update).

Calvet, J.P. (1994) Injury and development in polycystic kidney disease. Curr. Opin. Nephrol. Hypertens.

3, 340-348. (review and opinion).

Calvet, J.P. (1995) Abnormal epithelial cell proliferation in renal cyst formation and growth: The maturation arrest hypothesis. Kidney Int. 47, 715-716. (review of meeting talk).

Calvet, J.P. (1996) Principles of Molecular Biology as Applied to the Study of Disease. In Polycystic Kidney Disease (Watson, M.L. and Torres, V.E., eds.) Oxford University Press, Oxford, pp. 3-38. (book chapter).

Calvet, J.P. (1998) Molecular genetics of polycystic kidney disease. J. Nephrol. 11, 24-34. (basic science

review).

Calvet, J.P. (1998) Comprehensive sequence analysis. Science 282, 1057-1058. (software review).

Grantham, J.J. and Calvet, J.P. (2001) Polycystic kidney disease: In danger of being X-rated? Proc. Natl.

Acad. Sci. USA 98, 790-792. (editorial commentary).

Calvet, J.P. and Grantham, J.J. (2001) The genetics and physiology of polycystic kidney disease. Sem.

Nephrol. 21, 107-123. (comprehensive review).

Calvet, J.P. (2002) Cilia in PKD — Letting it all hang out. J. Am. Soc. Nephrol. 13, 2614-2616. (invited

editorial review).

Maser, R.L., Magenheimer, B.S., Zien, C.A. and Calvet, J.P. (2003) Transient Transfection Assays for Analysis of Signal Transduction in Renal Cells. In Methods in Molecular Medicine, Renal Disease Techniques and Protocols. M.S. Goligorsky, ed. Humana Press, Totowa, N.J., pp. 205-217. (methods chapter).

Calvet, J.P. (2003) Ciliary signaling goes down the tubes. Nature Genetics 33, 113-114. (news & views).

Calvet, J.P. (2003) New insights into ciliary function: kidney cysts and photoreceptors. Proc. Natl. Acad.

Sci. USA 100, 5583-5585. (invited commentary).

Calvet, J.P. (2006) MEK inhibition holds promise for polycystic kidney disease. J. Am. Soc. Nephrol.

17(6):1498-1500. (editorial).

Calvet, J.P. (2008) Strategies to inhibit cyst formation in ADPKD. Clin. J. Am. Soc. Nephrol. 3, 1205-1211

(summary of invited talk). Abstracts

Calvet, J.P., Laufer, H., and Krider, H. (1976) Repetitive nucleotide sequences in the Chironomus genome. J. Cell Biol. 70, 291a (Abstract).

Calvet, J.P. and Pederson, T. (1976) Characterization of double-stranded sequences in HeLa cell heterogeneous nuclear RNA-ribonucleoprotein particles. J. Cell Biol. 70, 291a (Abstract).

Calvet, J.P. and Pederson, T. (1977) Heterogeneous nuclear RNA packaging proteins: Role in modulating the formation of hnRNA secondary structure. J. Cell Biol. 75, 414a (Abstract).

Calvet, J.P. and Pederson, T. (1977) Messenger RNA processing: Ribonucleoprotein organization of double-stranded RNA sequences in HeLa cell heterogeneous nuclear RNA. J. Cell Biol. 75, 414a (Abstract).

Calvet, J.P. and Pederson, T. (1978) Psoralens can be used to probe hnRNA secondary structure in living HeLa cells. J. Cell Biol. 79, 345a (Abstract).

Calvet, J.P. and Pederson, T. (1981) Interactions between small nuclear RNAs and high molecular weight precursors of mRNA and rRNA shown by cross-linking with psoralen in vivo. J. Cell Biol. 91, 137a (Abstract).

Calvet, J.P. and Myers, J.A. (1982) Small nuclear RNA U4 is a major psoralen cross-linked RNA species in HeLa cell nuclei. J. Cell Biol. 95, 479a (Abstract).

Calvet, J.P. and Myers, J.A. (1983) In vivo structure analysis of the small nuclear RNA U1 using psoralen cross-linking. Fed. Proc. 42, 1882 (Abstract).

Calvet, J.P. and Myers, J.A. (1983) In vivo secondary structure and intermolecular base-pairing interactions involving the small nuclear RNAs analyzed by psoralen cross-linking. RNA Processing, Cold Spring Harbor Laboratory, p. 21 (Abstract).

Smardo, F.L., Jr. and Calvet, J.P. (1983) Identification of a small RNA that hybridizes specifically with high molecular weight cytoplasmic RNA of HeLa cells. J. Cell Biol. 97, 358a (Abstract).

Smardo, F.L., Jr. and Calvet, J.P. (1984) Characterization of a 4S cytoplasmic RNA that hybridizes to 28S ribosomal RNA of HeLa cells. Fed. Proc. 43, 2514 (Abstract).

Calvet, J.P., Smardo, F.L., Jr. and Maser, R.L. (1984) Small RNAs that do and do not interact with ribosomal RNA. RNA Processing, Cold Spring Harbor Laboratory, p.29 (Abstract).

Maser, R.L. and Calvet, J.P. (1984) Nucleolar-specific U3 RNA interacts with nonribosomal nuclear RNA in vivo. J. Cell Biol. 99, 144a (Abstract).

Smardo, F.L., Jr. and Calvet, J.P. (1984) Base-pairing interactions between small nuclear RNAs and adenovirus 2 mRNA precursors. J. Cell Biol. 99, 144a (Abstract).

Maser, R.L. and Calvet, J.P. (1986) Nucleolar-specific U3 snRNA psoralen crosslinks to 28S-sized nonribosomal RNA. RNA Processing, Cold Spring Harbor Laboratory, p. 114 (Abstract).

Kunkel, G.R., Maser, R.L., Calvet, J.P. and Pederson, T. (1986) U6 small nuclear RNA is transcribed by RNA polymerase III. RNA Processing, Cold Spring Harbor Laboratory, p. 108 (Abstract).

Maser, R.L. and Calvet, J.P. (1987) U3 snRNA psoralen cross-links to RNA hybrid-selected by ETS/18S ribosomal RNA sequences. RNA Processing, Cold Spring Harbor Laboratory, p. 119 (Abstract).

Cowley, B.D., Jr., Smardo, F.L., Jr., Grantham, J.J. and Calvet, J.P. (1987) Elevated c-myc expression in mouse polycystic kidney disease. Kid. Int. 31, 163 (Abstract).

Maser, R.L. and Calvet, J.P. (1987) U3 snRNA psoralen cross-links to RNA hybrid-selected by ETS/18S ribosomal RNA sequences. J. Cell Biol. 105, 68a (Abstract).

Harding, M.A., Calvet, J.P., Adamson, E.D. and Andrews, G.K. (1987) The heat shock response in HeLa cells is accompanied by elevated expression of the c-fos proto-oncogene. J. Cell Biol. 105, 106a (Abstract).

Cowley, B.D., Jr., Grantham, J.J. and Calvet, J.P. (1988) Elevated proto-oncogene expression following acute renal injury. Kid. Int. 33, 152 (Abstract).

Cowley, B.D., Jr., Grantham, J.J. and Calvet, J.P. (1988) Elevated proto-oncogene expression in polycystic kidney disease. FASEB J. 2, A839 (Abstract).

Harding, M.A. and Calvet, J.P. (1988) Translational effects on the stability of c-fos and c-myc mRNAs. J. Cell Biol. 107, 100a (Abstract).

Maser, R.L. and Calvet, J.P. (1988) U3 snRNA interacts with the external transcribed spacer of the human ribosomal RNA precursor near the +414 early processing site. J. Cell Biol. 107, 320a (Abstract).

Gattone, V.H., Fu-wen, N., Andrews, G.K., Klein, R.M. and Calvet, J.P. (1989) Reduced ontogenic expression of epidermal growth factor (EGF) in a murine model of renal cystic disease. Kid. Int. 35, 311 (Abstract).

Grantham, J., Uchic, M., Mangoo-Karim, R., Shumate, W. and Calvet, J. (1989) Net fluid secretion by mammalian renal epithelial cells: Stimulation by cAMP in polarized cultures derived from established renal cell lines and from normal and polycystic kidneys. Clin. Res. 37, 612a (Abstract).

Calvet, J.P., Harding, M.A., Gattone, V.H. and Grantham, J.J. (1990) Localization of overexpressed c-myc mRNA in the C57Bl/6J cpk murine model of polycystic kidney disease. J. Am. Soc. Nephrol. 1, 623 (Abstract).

Harding, M.A., Chadwick, L.J., Gattone, V.H. and Calvet, J.P. (1990) The SGP-2 gene is developmentally regulated in the mouse kidney and is persistently expressed in collecting duct cysts in polycystic kidney disease. J. Am. Soc. Nephrol. 1, 631 (Abstract).

Maser, R.L., Magenheimer, B.S. and Calvet, J.P. (1991) Identification of differentially expressed mRNAs in polycystic kidneys of the cpk mouse. J. Am. Soc. Nephrol. 2, 257 (Abstract).

Rankin, C.A., Grantham, J.J. and Calvet, J.P. (1991) c-fos expression is hypersensitive to serum-stimulation in cultured cystic kidney cells from the C57BL/6J-cpk mouse. J. Am. Soc. Nephrol. 2, 259 (Abstract).

Lowden, D.A., Merlino, G.T., Calvet, J.P., Barash, B.D. and Gattone, V.H. (1991) Renal pathology in transforming growth factor-alpha (TGFα) transgenic mice. J. Am. Soc. Nephrol. 2, 440 (Abstract).

Maser, R.L., Magenheimer, B.S. and Calvet, J.P. (1992) Plasma glutathione peroxidase is a major kidney mRNA and is differentially expressed in normal and polycystic cpk kidneys. J. Am. Soc. Nephrol. 3, 299 (Abstract).

Lindemann, G.W., Foo-Hurwitz, I., Magenheimer, B.S., Rankin, C.A. and Calvet, J.P. (1992) Isolation of differentially expressed cDNAs in polycystic kidneys of the cpk mouse by subtractive cloning. J. Am. Soc. Nephrol. 3, 298 (Abstract).

Maser, R.L., Magenheimer, B.S., Lindemann, G.W., Hou, X., Rankin, C.A. and Calvet, J.P. (1993) Identification of differentially expressed cDNAs in the cystic kidneys of the C57BL/6J-cpk mouse. J. Am. Soc. Nephrol. 4, 817 (Abstract).

Magenheimer, B.S. and Calvet, J.P. (1993) Altered expression of the mRNAs for HNF-1α, HNF-1β, and HNF-4 transcription factors in the cystic kidneys of the C57BL/6J-cpk mouse. J. Am. Soc. Nephrol. 4, 817 (Abstract).

Foo, I., Davidow, C.J., Grantham, J.J. and Calvet, J.P. (1993) Effects of fibroblast-conditioned media on morphological change and expression of the SGP-2 gene in MDCK cells. J. Am. Soc. Nephrol. 4, 813 (Abstract).

Maser, R.L. and Calvet, J.P. (1993) Androgen-responsive gene expression in kidney and submandibular gland of cystic cpk mice. J. Am. Soc. Nephrol. 4, 817 (Abstract).

Rankin, C.A., Ziemer, D.M., Foo, I. and Calvet, J.P. (1993) Cultured cystic kidney cells from the C57BL/6J-cpk mouse show lower than normal proliferation and increased c-jun gene expression. J. Am. Soc. Nephrol. 4, 821 (Abstract).

Gattone, V.H., Kuenstler, K.A., Lindemann, G.W. and Calvet, J.P. (1994) Renal expression of a transforming growth factor-alpha (TGFα) transgene accelerates the progression of inherited polycystic kidney disease (PKD). J. Am. Soc. Nephrol. 5, 622 (Abstract).

Hou, X., Maser, R.L., Magenheimer, B.S. and Calvet, J.P. (1994) Identification of a novel gene that is abnormally expressed in the polycystic kidneys of the cpk mouse. J. Am. Soc. Nephrol. 5, 626 (Abstract).

Rankin, C.A., Suzuki, K., Ziemer, D.M., Calvet, J.P. and Nagase, H. (1994) Abnormally high levels of MMPs and TIMPs are synthesized by kidney cells cultured from C57BL/6J cpk mice. J. Am. Soc. Nephrol. 5, 626 (Abstract).

Davidow, C.J., Maser, R.L., Calvet, J.P. and Grantham, J.J. (1995) Cystic fibrosis transmembrane conductance regulator (CFTR) mediates transepithelial fluid secretion by human ADPKD cyst epithelium in vitro. J. Am. Soc. Nephrol. 6, 693.

Rankin, C.A., Suzuki, K., Ziemer, D.M., Grantham, J.J., Calvet, J.P. and Nagase, H. (1995) 72-kDa Gelatinase A (MMP-2) is found in cyst fluid of 32 human patients and three rodent models of PKD. J. Am. Soc. Nephrol. 6, 707.

Rankin, C.A., Sarras, M., Calvet, J.P. and DeLisle, R. (1995) The murine polycystic kidney disease gene, cpk, affects pancreatic development. J. Am. Soc. Nephrol. 6, 708.

Maser, R.L. and Calvet, J.P. (1996) Reduced expression of antioxidant and detoxicant enzyme genes in polycystic kidney disease (PKD). J. Am. Soc. Nephrol. 7, 1617.

Maser, R.L., Vassmer, D., Calvet, J.P. and Magenheimer, B.S. (1997) Early induction of heme oxygenase-1 (HO-1) in polycystic kidney disease (PKD). J. Am. Soc. Nephrol. 8, 376A.

Parnell, S.C. and Calvet, J.P. (1997) Phosphorylation of the PKD1 protein, polycystin-1. J. Am. Soc. Nephrol. 8, 378A.

Rankin, C.A., Ziemer, D.M., Tian, C., Itoh, Y., Nagase, H., Calvet, J.P. (1997) Matrix metalloproteinase-2 (MMP-2) expression in a murine model of polycystic kidney disease (PKD). J. Am. Soc. Nephrol. 8, 380A.

Maser, R.L., Vassmer, D., Magenheimer, B.S. and Calvet, J.P. (1998) Is induction of heme oxygenase-1 (HO-1) a protective mechanism in polycystic kidney disease (PKD)? J. Am. Soc. Nephrol. 9, 378A.

Parnell, S.C., Magenheimer, B.S., Maser, R.L., Rankin, C.A., Smine, A., Okamoto, T. and Calvet, J.P. (1998) The polycystic kidney disease-1 protein, polycystin-1, binds and activates heterotrimeric G-proteins. J. Am. Soc. Nephrol. 9, 380A.

Rankin, C.A., Maser, R.L. and Calvet, J.P (1998) Affinity-purified polycystin-1 antibodies for Western blotting and co-immuno-precipitation analysis. J. Am. Soc. Nephrol. 9, 381A.

Parnell, S.C., Magenheimer, B.S. and Calvet, J.P. (1999) PKA phosphorylation of the polycystin-1 protein. FASEB J. 13, A1406.

Maser, R.L., Sutters, M., Vassmer, D., Magenheimer, B.S. and Calvet, J.P. (1999) Polycystin-1 augments glucocorticoid induction of endogenous GST-Ya gene expression in stably transfected M-1 cells. J. Am. Soc. Nephrol. 10, 420A.

Sutters, M., Maser, R.L., Magenheimer, B.S., Gattone, V.H. and Calvet, J.P. (1999) Inducible expression of the C-terminal cytoplasmic domain of polycystin-1 in stably transfected M-1 mouse cortical collecting duct cells. J. Am. Soc. Nephrol. 10, 426A.

Calvet, J.P., Yamaguchi, T., Maser, R.L., Sutters, M. and Grantham, J.J. (2000) A stably transfected C-terminal polycystin-1 construct transforms the cAMP growth-responsive phenotype of M-1 cells. J. Am. Soc. Nephrol. 11, 387A.

Maser, R.L., Vassmer, D., Magenheimer, B.S. and Calvet, J.P. (2000) Reduced extracellular glutathione peroxidase enzyme and increased lipid peroxidation levels in polycystic kidney disease (PKD). J. Am. Soc. Nephrol. 11, 395A.

Parnell, S.C., Magenheimer, B.S., Maser, R.L. and Calvet, J.P. (2000) Polycytin-1 activation of c-Jun-N-terminal kinase and AP-1 is mediated by heterotrimeric G-proteins. J. Am. Soc. Nephrol. 11, 396A.

Parnell, S.C., Magenheimer, B.S., Maser, R.L. and Calvet, J.P. (2000) Polycystin-1 activation of c-Jun N-terminal kinase (JNK) and AP-1 is mediated by heterotrimeric G-proteins. FASEB J. 14, A1343.

Magenheimer, B.S., Rodova, M., Maser, R.L. and Calvet, J.P. (2001) Mutations affecting polycystin-1 C-terminal tail activation of the AP-1 transcription factor. J. Am. Soc. Nephrol. 12, 540A.

Nims, N., Magenheimer, B.S., Vassmer, D., Calvet, J.P. and Maser, R.L. (2001) Transmembrane domain analysis of polycystin-1. J. Am. Soc. Nephrol. 12, 541A.

Parnell, S.C., Magenheimer, B.S., Maser, R.L. and Calvet, J.P. (2001) Polycytin-1 activation of c-Jun-N-terminal kinase is mediated by Gi2 a nd G s ubunits of he te rotrime ric G prote ins . J. Am. Soc. Nephrol. 12, 543A.

Rodova, M., Islam, R., Maser, R.L. and Calvet, J.P. (2001) Initial characterization of the human PKD1 promoter and its induction by -catenin. J. Am. Soc. Nephrol. 12, 545A.

Yamaguchi, T., Wallace, D.P., Grantham, J.J. and Calvet, J.P. (2001) Changes in intracellular Ca2+ cause a phenotypic switch in the proliferative response of M-1 cells to cAMP. J. Am. Soc. Nephrol. 12, 548A.

Hein, R.C., Maser, R.L., Magenheimer, B.S. and Calvet, J.P. (2002) A chimeric polycystin-1 C-tail/PTH receptor construct constitutively activates AP-1 signaling through heterotrimeric G12/13 family proteins. J. Am. Soc. Nephrol. 13, 105A.

Magenheimer, B.S., Rodova, M., Slaughter, D.M., Maser, R.L. and Calvet, J.P. (2002) Polycystin-1 C-tail single amino acid mutations inhibit heterotrimeric G protein signaling. J. Am. Soc. Nephrol. 13, 18A.

Puri, S., Rodova, M., Islam, R., Maser, R.L. and Calvet, J.P. (2002) The human PKD1 promoter is regulated by Ets family transcription factors. J. Am. Soc. Nephrol. 13, 46A.

Yamaguchi, T., Wallace, D.P., Grantham, J.J. and Calvet, J.P. (2002) Decreased intracellular Ca2+, which causes an ADPKD-like switch in the proliferative response of M-1 cells to cAMP, is associated with increased activation of Src, B-Raf, and ERK, and increased B-Raf protein. J. Am. Soc. Nephrol. 13, 105A.

Nims, N., Vassmer, D., Magenheimer, B.S., Calvet, J.P. and Maser, R.L. (2002) Polycystin-1 transmembrane biogenesis. J. Am. Soc. Nephrol. 13, 105A.

Yamaguchi, T., Wallace, D.P., Grantham, J.J., and Calvet, J.P. (2003) Ca 2+ channel blockers transform normal renal epithelial cells to a cAMP growth-stimulation phenotype, and augment the mitogenic action of cAMP in ADPKD cyst epthelial cells. J. Am. Soc. Nephrol. 14, 576A.

Puri, S., Magenheimer, B.S., Zien, C.A., Walker, D.D., Maser, R.L., and Calvet, J.P. (2003) The polycystin-1 C-tail activates the calcineurin/NFAT pathway in a heterotrimeric G protein-dependent fashion. J. Am. Soc. Nephrol. 14, 576A.

El-Dahr, S., Dipp, S., Saifudeen, Z., Magenheimer, B.S., Puri, S., Calvet, J. (2004) p53 is a negative transcriptional regulator of the human polycystic kidney disease-1 (PKD-1) Gene. J. Am. Soc. Nephrol. 15, 219A.

Puri, S. and Calvet, J.P. (2004) The cyclooxygenase-2 (Cox-2) promoter is a target of polycystin-1 mediated signal transduction. J. Am. Soc. Nephrol. 15, 219A.

Magenheimer, B.S., St. John, P.L., Isom, K.S., Abrahamson, D.R., De Lisle, R.C., Wallace, D.P., Maser, R.L., Grantham, J.J., and Calvet, J.P. (2004) Cyclic AMP- and CFTR-dependent cystic dilation of embryonic renal tubules in metanephric organ culture. Am. Soc. Nephrol. 15, 651A.

Alcalay, N.I., Brantley, J.G., Sharma, M., Magenheimer, B.S., Calvet, J.P., and Vanden Heuvel, G.B. (2004) Ectopic expression of the homeobox gene Cux-1 rescues metanephric growth inhibition by cyclosporin A. Am. Soc. Nephrol. 15, 675A.

Magenheimer, B.S., Mehzabeen, N., and Calvet, J.P. (2005) Cyclic AMP-dependent ERK activation in metanephric organ culture in response to Ca2+ restriction and in wild-type vs. mutant Pkd1 kidneys. J. Am. Soc. Nephrol. 16, 584A.

Xiao, Z.S., Zhang, S.Q., Mahlios, J., Calvet, J.P., Bonewald, L.F., and Quarles, L.D. (2005) Polycystin-1: A novel mechanosensor in osteoblast/osteocytes coupled to Runx2. J. Am. Soc. Nephrol. 16, 585A.

Yamaguchi, T., Wallace, D.P., and Calvet, J.P. (2005) Inhibition of the calcineurin/NFAT signaling pathway allows cAMP-dependent stimulation of ERK in normal renal epithelial cells, mimicking the PKD phenotype. J. Am. Soc. Nephrol. 16, 587A.

Islam, M.R., Puri, S., Rodova, M., Magenheimer, B.S., Maser, R.L., and Calvet, J.P. (2006) Activation of the polycystic kidney disease-1 (PKD1) promoter by retinoic acid: Involvement of Sp1. J. Am. Soc. Nephrol. 17, 513A.

Xiao, Z.S., Zhang, S.Q., Mahlios, J., Zhou, G., Magenheimer, B.S., Maser, R.L., Calvet, J.P., Dallas, S.L., Guo, D.Y., Bonewald, L.F., and Quarles, L.D. (2006) Primary cilium and polycystin-1 in osteoblasts/osteocytes and associated abnormalities in skeletogenesis and Runx2 expression. J. Am. Soc. Nephrol. 17, 517A.

Magenheimer, B.S., Wallace, D.P., Maser, R.L., and Calvet, J.P. (2006) Cyclic AMP-stimulated cyst formation in heterozygous and homozygous Pkd1-deficient kidneys is dependent on CFTR-mediated chloride transport. J. Am. Soc. Nephrol. 17, 89A.

Wallace, D.P., Reif, G.A., Hempson, S.J., Quante, M.T., Calvet, J.P., and Yamaguchi, T. (2006) Periostin (osteocyte specific factor-2) increases ADPKD cell proliferation through the activation of v-integrin and -catenin/TCF signaling pathways. J. Am. Soc. Nephrol. 17, 703A.

Magenheimer, B.S., Wall, S.M, and Calvet, J.P. (2007) Role of CFTR and NKCC1-dependent fluid secretion in early cyst formation in mouse embryonic kidneys. J. Am. Soc. Nephrol. 18, 50A.

Xiao, Z., Zhang, S., Magenheimer, B.S., Calvet, J.P., and Quarles, L.D. (2007) Polycystin-1 stimulation of Runx2-II expression is mediated through the calcium-PI3K-Akt pathway. J. Am. Soc. Nephrol. 18, 127A.

Sundar, S.V., Magenheimer, B.S., and Calvet, J.P. (2007) Tuberin-polycystin-1 interactions and co-localization in primary cilia. J. Am. Soc. Nephrol. 18, 130A.

Nishii, K., Nagaoka, K., Yoshihara, D., Takahashi, H., Kogo, H., Yamada, K., Kowa, H., Yanagihara, I., Kurahashi, H., Yamashita, T., Yamaguchi, T., Calvet, J.P., Wallace, D.P., and Nagao, S. (2007) The mutation in NPHP3 associated with renal cysts in pcy mice disrupts protein interaction between NPHP3 and NPHP1 in cilia. J. Am. Soc. Nephrol. 18, 359A.

Lu, H., Wallace, D.P., Lei, X., Klaassen, C.D., and Calvet, J.P. (2007) C/EBP, a tra ns cription fa ctor involved in cell proliferation, is highly expressed in cystic tissue of human ADPKD kidneys. J. Am. Soc. Nephrol. 18, 778A.

Magenheimer, B.S., Sundar, S.V., Reif, G.A, Wallace, D.P., Maser, R.L., Georg, G.I., Tash, J.S., and Calvet, J.P. (2008) The Lonidamine Derivative Gamendazole Inhibits Cyst Growth in Pkd1-Deficient Kidneys in Metanephric Culture by Targeting both Fluid Secretion and Cell Proliferation. J. Am. Soc. Nephrol. 19, 26A.

Parnell, S.C. and Calvet, J.P. (2008) Protein Phosphatase-1 Inte ra cts with a nd De phos phoryla te s Polycystin-1. J. Am. Soc. Nephrol. 19, 131A.

Nguyen, A.-N., Wallace, D.P., Magenheimer, B.S., Jansson, K.P., Calvet, J.P., and Blanco, G. (2008) Ouabain exacerbates cystogenesis in ADPKD via the EGFR-Src-MEK pathway. J. Am. Soc. Nephrol. 19, 364A.

Sundar, S.V., Magenheimer, B.S., Reif, G.A., Wallace, D.P., Georg, G.I., Tash, J.S., and Calvet, J.P. (2008) Gamendazole Inhibits ADPKD Cell Proliferation and Induces Cytoskeletal Rearrangements. J. Am. Soc. Nephrol. 19, 364A.

Parnell, S.C. and Calvet, J.P. (2009) Mutation of the Protein Phosphatase-1 Binding Motif Disrupts Polycystin-1 Dephosphorylation. J. Am. Soc. Nephrol.

Sundar, S.V., Puri, S., Donnelly, A.C., Blagg, B.S.J., Reif, G.A., Wallace, D.P., and Calvet, J.P. (2009) The Novel Hsp90 Inhibitor KU−174 Targets the mTOR Pathway in ADPKD Cells and Reduces In Vitro Cyst Formation. J. Am. Soc. Nephrol.

Shah, D.B., Magenheimer, B.S., Sundar, S.V., Georg, G.I., and Calvet, J.P. (2009) A Potent Lonidamine Derivative JWS-1-190 Inhibits Cell Proliferation and Cyst Formation in Polycystic Kidney Disease. J. Am. Soc. Nephrol.

Anuj Gupta, Veena Puri, James P. Calvet and Sanjeev Puri. (2010) E2F family transcription factors regulate the polycystic kidney disease-1 gene promoter through an Ets binding element. J. Am. Soc. Nephrol.

Daisuke Yoshihara, Hiroki Kurahashi, Miwa Morita, Masanori Kugita, Yoshiyuki Hiki, Harold M. Aukema, Tamio Yamaguchi, James P. Calvet, Darren P. Wallace, and Shizuko Nagao. (2010) Thiazolidinedione Ameliorates Autosomal Recessive Polycystic Kidney and Liver Disease in PCK Rat, An Orthologous Model of Human ARPKD. J. Am. Soc. Nephrol.

Brenda S. Magenheimer, Shirin Sundar, Dar B. Shah, Sumanth Mulamalla, Gail Reif, Darren P. Wallace, Joseph S. Tash, Gunda I. Georg and James P. Calvet. (2010) Lonidamine Derivatives Have Properties Expected of Effective Drugs for Treating ADPKD. J. Am. Soc. Nephrol.

Cibele S. Pinto, Brenda S. Magenheimer, Gail A. Reif, James P. Calvet, Darren P. Wallace. (2010) Role of phosphodiesterases on cAMP-dependent ERK activation and cyst formation in PKD. J. Am. Soc. Nephrol.

Katherine Swenson-Fields, Darren P. Wallace, Carolyn J. Vivian, Cibele S. Pinto, Gail A. Reif, Brenda S. Magenheimer, James P. Calvet, Timothy A. Fields. (2010) Wnt5a is upregulated in ADPKD cyst epithelial cells and stimulates cAMP production and in vitro cyst growth. J. Am. Soc. Nephrol.

Robin L. Maser, Andreea Chiselita, Brenda S. Magenheimer, James P. Calvet, Donna M. Ziemer, Melissa Johnson, and Dianne Vassmer. (2010) Curcumin inhibits cAMP-mediated cystic dilation in Pkd1-deficient kidneys in metanephric organ culture. J. Am. Soc. Nephrol.

Stephen C. Parnell, Sanjeev Puri, Lance C. Brandenburgh, Darren P. Wallace, and James P. Calvet. (2010) ADPKD-Associated F4145V Mutation in Polycystin-1 Disrupts its Dephosphorylation by Protein Phosphatase-1. J. Am. Soc. Nephrol.

Brenda S. Magenheimer, Shirin Sundar, Sumanth Mulamalla, Monica K. Johnson, Gail Reif, Darren P. Wallace, and James P. Calvet. (2011) Lonidamine Reduces Cyst Size in Polycystic Kidney Disease. ASN Abstracts 2011.

Daisuke Yoshihara, Masanori Kugita, Hiroki Kurahashi, Miwa Morita, Yoshiyuki Hiki, Tamio Yamaguchi, Harold M. Aukema, Darren P. Wallace, James P. Calvet, Takafumi Toyohara, Takaaki Abe, and Shizuko Nagao. (2011) Global Gene Expression Profiling in Kidneys of PPAR- Agonis t-Treated PCK Rats, an Orthologous Model of Human ARPKD. ASN Abstracts 2011.

Xia Zhou, Lucy X. Fan, Wei Liu, William E. Sweeney, James P. Calvet, Ellis D. Avner, Xiaogang Li. (2011) Cilia Defects and Polyploidy Linked to Low PC1 Expression in Kidney Epithelia Cells Are Mediated by Increased Expression of Sirt2 and HDAC6. ASN Abstracts 2011.

Stephen C. Parnell, Brenda S. Magenheimer, Robin L. Maser, Mallory A. Havens, Lynn Magenheimer, Michelle Hastings, James P. Calvet. (2012) A Single Amino Acid Deletion in the Polycystin-1 (PC1) C-Tail Affects G-Protein Signaling and Causes PKD in Mice. ASN Abstracts 2012.

Tarundeep Kaur, Nidhi Mahajan, Veena Puri, Vivekanand Jha, Surinder Kumar Singla, James P. Calvet, Sanjeev Puri. (2012) Transforming Growth Factor Beta: A Molecular Target of Polycystin-1 and Nuclear Factor of Activated T-Cell Signaling. ASN Abstracts 2012.

Kyle Jansson, Brenda S. Magenheimer, James P. Calvet, Gustavo Blanco. (2012) Over-Expression of the Polycystin-1 (PC1) C-Tail Enhances the Sensitivity of M-1 Cells to the Effects of Ouabain. ASN Abstracts 2012.

Patents

DNA Encoding Mouse Phosphotriesterase-Related Protein; Patent # 5552313; Inventors: Calvet, J.P., Hou, X., Magenheimer, B.S., and Maser, R.L. Issue date: September 3, 1996.

Lonidamine Analogs and Treatment of Polycystic Kidney Disease; Application #12/395,521; Inventors: Georg, G.I., Tash, J.S., Chakrasali, R., Jakkaraj, S.R., and Calvet, J.P. Filing date: February 27, 2009.

Novobiocin Analogues and Treatment of Polycystic Kidney Disease; Application #12/894,654; Inventors: Calvet, James, P., Blagg, Brian, S., J., Sundar, Shirin, V., and Magenheimer, Brenda, S. Filing date: September 30, 2010.

Departmental and Institutional Activities

Participant in Molecular Genetics Symposium, Research Week, October 6, 1983; presented talk, "The Regulation of Eukaryotic Gene Expression by RNA Processing".

Recruiting Seminar, William Jewell College, October 18, 1983.

Director of Graduate Studies in Biochemistry and Molecular Biology (1984-1986; 1993-1995).

Member, KUMC Institutional Graduate Committee (1984-1986; 1993-1995).

Member, Standing Committee on Graduate Faculty Appointments and Authorizations (1984-1985).

Member, Departmental Immunobiochemist Search Committee (1985).

Member, LCME Subcommittee on Research (1985).

Member, Graduate School Five-Year Review Committee for Biochemistry (1986).

Chairman, Graduate School Five-Year Review Committee for Pathology (1986).

Member, Student Health Policy Committee (1986).

Basic Science Faculty Representative, Medical Class Selection Committee (1988-1990, 1992, 1993; Fall admissions 1989-1991).

Member, M.D./Ph.D. Advisory Committee (1989-1992).

Member, School of Medicine Academic Committee (1990-1993).

Member, KUMC Graduate Travel Scholarship Committee (1991-1994, 1996-1999).

Chairman, M.D./Ph.D. Academic Review Subcommittee (1991-1992).

Recruiting Seminar, William Jewell College, March 6, 1991.

Judge, KUMC Student Research Forum (1991, 1994-1996, 2006).

Member, KUMC Radiation Safety Committee (1991-1994).

Member, Philip Newmark Award Committee (1992).

Member, Search Committee for Senior Associate Dean for Academic Affairs (1994).

Member, Basic Science Core-Curriculum Feasibility and Planning Committee (1994-1995).

Chairman, Departmental Search Committee for Molecular Geneticist (recruited Dr. H. Serizawa) (1995-1996).

Member, IGPBS Modules 3 and 5 Committees (1996-1998).

Course Director, Module 5 “Regulation of Gene Expression and Development” of the IGPBS (1996).

Member, Search Committee for Chairman of the Department of Microbiology, Immunology, and Molecular Genetics (recruited Dr. Bill Narayan) (1994-1995; 1997-1999).

Member, Departmental Search Committee for Developmental Molecular Geneticist (1997-1998) (recruited Dr. Ken Peterson).

Member, Departmental Search Committee for X-Ray Crystallographer (recruited Dr. M. Sundaramoorthy) (1997-1999).

Chairman, Departmental P&T Committee (1999-2000, 2000-2001).

Member (Dean’s appointment), School of Medicine Promotion and Tenure Committee (1997-1999).

Instructor, Health Careers Pathways Program (1990-2002).

Member, Chair Review Committee for the Department of Pharmacology, Toxicology, and Therapeutics, (April/May, 2000).

Presented talk at the Grant Writing Workshop entitled “How to Generate Novel Ideas,” April 6, 2000.

Mini Medical School presenter, September 26, 2000: “Human Genetic Disease: When the Blueprint of Life Gets Smudged.”

Member, Search Committee for the Dept. of Molecular and Integrated Physiology (recruited Dr. Gustavo Blanco) (2000-2001).

Co-Director, Murphy Society (1999-2002).

Chairman, Pathology Course Review Committee (2001-2002).

Member, School of Medicine Research Committee (1993-1996; 1999-2002; 2002-2005, 2006-2009).

Chairman, School of Medicine Research Committee (2001-2002).

Member, Faculty Assembly Research Committee (2001-2002; 2005-2006).

Chairman, Genetics Curriculum Subcommittee of the Education Council (2002-2004).

Member, Chair Review Committee for the Department of Anatomy and Cell Biology (2004).

Member, Departmental Search Committee for Molecular Geneticist (2004-2006) (recruited Dr. Joe Fontes).

Co-Chair, LCME Self-Study Committee: Institutional Setting: Academic Setting (2004-2005).

Member, School of Medicine Faculty Council (1992-1993; 2000-2006).

Member, Biomedical Research Training Program Committee (2005, 2006).

Co-Course Director, Medical Biochemistry 801/802 (2000-2001).

Course Director, Medical Biochemistry 801/802 (2001-2006) (Student Voice Award for the Best First-Year Course, 2003-2004).

Member, Years 3-4 Curriculum Subcommittee (1995-1997, 2006-2007).

Judge, Capitol Research Summit, Topeka, KS, February 17, 2011.

Interim Director, Kidney Institute (2009-2011) (jointly recruited Dr. Reena Rao and Dr. Pamela Tran).

Member, Search Committee for Director of the Kidney Institute and Director of the Division of Nephrology and Hypertension (2009-2010) (recruited Dr. Alan Yu).

Member, Search Committee for Chair of the Dept. of Microbiology, Immunology, and Molecular Genetics (2010-2011).

Ad Hoc Faculty Investigation and Hearing Committee, 2012.

Chair, Faculty Search Committee for the Department of Cancer Biology (2012) (recruited Dr. Joan Lewis-Wambi).

Temporary Interim Vice Chancellor for Research and Senior Associate Dean for Research and Graduate Studies (for Paul Terranova), April 2-16, 2012.

Workshop Presenter, Emotional Maturity in Science. Wednesday, November 28, 2012.

Member, Study Section for Frontiers/Clinical/Lied Pilot Grants. Friday, December 14, 2012.

Judge, Capitol Research Summit, Topeka, KS, February 14, 2013.

Present Activities – Departmental and Institutional

Member, Department of BMB AP&T Committee (1994-present).

Member, Department of BMB Graduate Committee (1983-1986; 1992-1996; 1999-present).

Module Director, Genetics & Neoplasia (2006-present).

Member, School of Medicine Phase I Curriculum Committee (2005-present).

Member, School of Medicine M.D./Ph.D. Committee (2008-present).

Co-Leader, Cancer Biology Program, KU Cancer Center (2009-present).

Deputy Director, Kidney Institute, University of Kansas Medical Center (2012-present).

Member, Postdoctoral Advisory/Travel Committee (2012-present).

National/International Activities

Student, Embryology Course and Post-Course Research, Marine Biological Laboratory, Woods Hole, MA; summer 1971.

Member, Local Arrangements Committee for the 24th annual meeting of the American Society for Cell Biology, Kansas City, MO, 1984.

Invited Lecturer, Department of Biology, University of Puerto Rico, Rio Piedras, PR, September 12-23, 1983; presented 6 lectures and conducted 2 laboratory sessions in the graduate course, Modern Topics in Biology (Molecular Biology).

NIH NIDDK Consultant, "Future Research Strategies Once the Polycystic Kidney Disease Gene is Isolated", March 20, 1987.

Instructor, Physiology: Cell and Molecular Biology Course, Marine Biological Laboratory, Woods Hole, MA, July 11- August 6, 1988.

Workshop Speaker, "An Introduction to Molecular Biology for Nephrologists", American Society of Nephrology, Washington, DC, November 30- December 1, 1989; presented talks, "Isolation of DNA and RNA" and "cDNA isolation/cDNA libraries", and conducted two problem-solving sessions.

Invited Symposium Speaker, "Factors Affecting the Progression of Renal Cystic Disorders", sponsored by the National Kidney Foundation; Washington, DC, Nov. 30, 1990; presented talk, "Mechanisms of Increased Cell Proliferation in Renal Disease".

Invited Symposium Speaker, "Cell and Molecular Biology of Basement Membranes in Health and Disease" sponsored by the International Society of Nephrology and the National Institutes of Health; Airlie, VA, September 19-22, 1991; presented talk, "Renal Cystic Disease".

Invited Symposium Speaker, "Molecular Genetics of Kidney Diseases" sponsored by the NIDDK; Reston, VA, March 28-30, 1992; presented talk in session entitled "Models of Genetic Diseases".

Invited Symposium Speaker, "The Fifth International Workshop on Polycystic Kidney Disease" sponsored by the Polycystic Kidney Research Foundation; Kansas City, MO, June 19-20, 1992; presented talk, "Proto-Oncogenes and Abnormalities of Differentiation in PKD".

Workshop Speaker, "Molecular Approaches to Urologic Diseases" sponsored by the American Urological Association Office of Education; Houston. TX, August 7-9, 1992; gave the after-dinner (keynote) presentation, entitled "Genes and the Development of Polycystic Kidney Disease".

Scientific Advisory Board, Polycystic Kidney Disease Foundation, Kansas City, MO (1993-1999).

Invited Symposium Speaker, "Abnormal Epithelial Cell Proliferation in Renal Cyst Formation and Growth: The Maturation Arrest Hypothesis"; Forefronts in Nephrology '94, Molecular Basis of Renal Cystic Disease, sponsored by the International Society of Nephrology, Niagara-on-the-Lake, Ontario, Canada, May 13-15, 1994.

NIH NIDDK Workshop to Plan the Future of PKD Research; Bethesda, MD; September

19, 1994.

NIH Biochemistry Study Section (Ad Hoc), February 22-24, 1995.

Conference Participant; 6th Annual PKD Conference; sponsored by the Polycystic Kidney Research Foundation, Washington, DC, June 23-25, 1995; presented talk entitled “Cell Biology 101".

Invited Grand Rounds Speaker, “Polycystic Kidney Disease”; Southern Illinois University School of Medicine, Springfield, IL, November 7, 1995.

Invited Symposium Speaker, “Cell Proliferation and Gene Expression Events Following Acute Renal Injury”; Symposium entitled “Cell Proliferation, Tissue Repair and Outcome of Toxic Injury”; 16th Annual Meeting of the American College of Toxicology, Vienna, VA, November 12-15, 1995.

Conference Participant; 7th Annual PKD Conference; sponsored by the Polycystic Kidney Research Foundation, Huntington Beach, CA, June 21-23, 1996; presented talk entitled “Cell Biology 101".

Conference Participant; 8th Annual PKD Conference; sponsored by the Polycystic Kidney Research Foundation, Nashville, TN, June 13-15, 1997; presented talk entitled “Cell Biology 101".

Session Chairman, “Cell Biology of PKD”, 1997 NIDDK Workshop on Polycystic Kidney Disease, September 10-11, 1997, Crystal City, VA; presented overview for session and presented a poster.

NIH Special Study Section ZRG4 GRM(07)L, NRSA Postdoctoral Fellowships (F32); January 12, 1998.

PKRF grant reviews, American Society of Nephrology, Miami Beach, FL, November 4, 1999.

PKRF grant reviews, American Society of Nephrology, Toronto, Canada, October 12, 2000.

External Scientific Advisory Board, Case Western Reserve University School of Medicine, NIH PKD Center, (1999-2006).

Keynote Speaker at the FASEB Summer Research Conference on “Molecular Mechanisms & Therapeutic Insights in Polycystic Kidney Disease,” August 10-15, 2002, Tucson, AZ. Keynote address entitled, “Mechanisms of Cystogenesis.” Also presented a regular session talk entitled, “Transcriptional Regulation of the PKD1 Gene.”

NIH Special Emphasis Panel ZDK1 GRB-6 (J1), “Program Project Applications in Kidney Development and PKD,” December 11, 2003, Linthicum, MD.

Invited Symposium Speaker, “Polycystin Signal Transduction”; Symposium entitled “International Symposium on Polycystic Kidney Disease”; one of 16 international invited speakers; Shanghai, Peoples' Republic of China, May 23-24, 2004.

Chair, NIH Special Emphasis Panel ZRG1 RUS-D 03, “PKD Science,” teleconference July 30, 2004.

Invited Discussion Meeting Speaker, “Cell Signalling in Polycystic Cells,” January 20, 2005, London, England.

Invited Speaker at the TSC/PKD Workshop “Polycystic Kidney Disease and Tuberous

Sclerosis Complex: Building Bridges to the Future” March 9-10, 2006, Chicago, IL. Presented talk “Ca2+ and cAMP signaling in PKD.”

Consultant for Clinical Tools, Inc. for their GeneticSolutions.com curriculum for medical students (2006-2007).

NIH CMBK Study Section (Ad Hoc), February 12, 2007.

Invited talk “Calcium and cAMP in PKD Cells and Embryonic Kidneys” ISN/Nature Genetics Forefronts Symposium on Nephrogenetics: From Development to Physiology” Danvers, MA, March 10, 2007.

Invited talk “Strategies to Inhibit Cyst Formation” Am. Soc. Nephrol. meeting in the Clinical Nephrology Conference “New Insights, Treatments, and Management Strategies for ADPKD” San Francisco, CA, November 3, 2007.

Site Visit Grant Reviewer, Fondazione Telethon, Milan, Italy, November 14-15, 2007.

Invited Symposium Speaker, “Cyclic AMP and MAPK Signaling in PKD”; Symposium entitled “Molecular Targets in Renal Disease”; Bamberg, Germany, October 17-19, 2008.

Invited talk “Cellular, In Vitro, and Ex Vivo Models of PKD” Polycystic Kidney Disease Drug Discovery: Challenges and Opportunities, April 8, 2010, Chicago, Ill.

NIH CMBK (Cellular and Molecular Biology of the Kidney) Study Section (Regular Member), 2007-2011.

Lillian Jean Kaplan International Prize for Advancement in the Understanding of Polycystic Kidney Disease awarded jointly by the International Society of Nephrology and PKD Foundation; presented invited talk “Polycystic Kidney Disease: A Calcium Signaling Disorder” Vancouver, B.C., April 11, 2011.

John C. Davis Memorial Lecture (keynote address) “Polycystic Kidney Disease: A Calcium Signaling Disorder” at the Department of Molecular Biosciences Graduate Student Symposium, University of Kansas, Lawrence, KS, August 17, 2012.

Speaker and Panel Discussant, PKD Webinar Virtual Convention, "What's New in PKD: A Live Broadcast Day of PKD Learning" Saturday, March 9, 2013.

Present Activities – National/International

Member, External Advisory Committee, P30 PKD Center, Univ. Alabama, Birmingham, 2007-present. (Chair, 2007, 2008, 2011, 2013)

Member, External Advisory Comm., Creighton Univ. Cancer and Smoking Diseases Research Program – LB595, 2008-present.

Co-organizer, FASEB Summer Research Conference: “Polycystic Kidney Disease, From Bench to Bedside” Saxtons River, VT, June 26–July 1, 2011.

Co-organizer, FASEB Summer Research Conference: “Polycystic Kidney Disease, From Bench to Bedside” Lucca, Italy, August 2014.

Member, Award Selection Committee for the Lillian Jean Kaplan International Prize for

Advancement in the Understanding of Polycystic Kidney Disease (2012). Meetings/Workshops/Conferences Attended

Mini-Symposium on Developmental Biology, Woods Hole, MA, July 7, 1981; presented invited symposium talk.

West Central States Biochemistry Conference, Lincoln, NE, October 23-24, 1981; presented platform talk.

American Society for Cell Biology, Anaheim, CA, November 9-13, 1981; presented platform talk.

RNA Processing, Cold Spring Harbor, NY, May 19-23, 1982.

American Society for Cell Biology, Baltimore, MD, November 30-December 4, 1982; chaired session entitled "RNA Processing, Ribonucleoprotein", and presented platform talk.

RNA Processing, Cold Spring Harbor, NY, May 18-22, 1983; presented platform talk.

American Society of Biological Chemists, San Francisco, CA, June 5-9, 1983; presented poster.

American Society for Cell Biology, San Antonio, TX, November 29-December 3, 1983; presented poster.

RNA Processing, Cold Spring Harbor, NY, May 16-20, 1984; presented poster.

West Central States Biochemistry Conference, Manhattan, KS, October 26-27, 1984; presented poster.

American Society for Cell Biology, Kansas City, MO, November 12-16, 1984; presented two posters.

RNA Processing, Cold Spring Harbor, NY, May 14-18, 1986; presented two posters.

West Central States Biochemistry Conference, Lawrence, KS, October 24-25, 1986; session chairman.

RNA Processing, Cold Spring Harbor, NY, May 13-17, 1987; presented poster.

Etiology and Pathogenesis of Polycystic Kidney Disease, Deerfield, IL, September 17-18, 1987; invited symposium speaker.

American Society for Cell Biology, St. Louis, MO, November 16-20, 1987; presented two posters.

Workshop on Control of Renal Growth, Bethesda, MD, March 7-8, 1988.

Baxter/PKRF Round Table, Overland Park, KS, September 16-17, 1988; invited speaker.

American Society for Cell Biology and American Society for Biochemistry and Molecular Biology, San Francisco, CA, January 29- February 2, 1989; presented two posters.

American Society of Nephrology, Washington, DC, Nov. 30- Dec. 1, 1989; Workshop

Speaker, "An Introduction to Molecular Biology for Nephrologists," presented talks, "Isolation of DNA and RNA" and "cDNA isolation/cDNA libraries", and conducted two problem-solving sessions.

National Kidney Foundation/ American Society of Nephrology, Washington, DC, November 29- December 3, 1990; presented one talk and was an author on another presented talk.

International Symposium on Cell and Molecular Biology of Basement Membranes in Health and Disease, Airlie, VA, September 19-22, 1991; presented one invited talk and one poster.

American Society of Nephrology, Baltimore, MD, November 17-20, 1991; author on three presented talks.

NIDDK Workshop "Molecular Genetics of Kidney Diseases", Reston, VA, March 28-30, 1992; presented talk.

The Fifth International Workshop on Polycystic Kidney Disease, Kansas City, MO, June 19-20, 1992; presented talk.

Conference sponsored by the American Urological Association Office of Education; Houston. TX, August 7-9, 1992; Workshop Speaker, "Molecular Approaches to Urologic Diseases," gave the after-dinner keynote presentation entitled "Genes and the Development of Polycystic Kidney Disease."

American Society of Nephrology, Baltimore, MD, November 15-18, 1992; author on one presented talk and one poster.

American Society of Nephrology, Boston, MA, November 14-17, 1993; author on one poster.

Forefronts in Nephrology '94, Molecular Basis of Renal Cystic Disease, sponsored by the International Society of Nephrology, Niagara-on-the-Lake, Ontario, Canada, May 13-15, 1994; Invited Symposium Speaker, "Abnormal Epithelial Cell Proliferation in Renal Cyst Formation and Growth: The Maturation Arrest Hypothesis."

American Society of Nephrology, Orlando, FL, October 26-29, 1994; author on one presented talk and two posters.

6th Annual PKD Conference; sponsored by the Polycystic Kidney Research Foundation, Washington, DC, June 23-25, 1995; presented talk entitled “Cell Biology 101."

Grand Rounds, Department of Medicine, Southern Illinois University School of Medicine, Springfield, IL, November 7, 1995.

American College of Toxicology 16th Annual Meeting, Vienna, VA, November 12-15, 1995; invited symposium speaker.

7th Annual PKD Conference; sponsored by the Polycystic Kidney Research Foundation, Huntington Beach, CA, June 21-23, 1996; presented talk entitled “Cell Biology 101."

American Society of Nephrology, New Orleans, LA, November 3-6, 1996; author on one poster; also attended the Basic Science Conference: Renal Developmental Biology, November 6-9, 1996.

8th Annual PKD Conference; sponsored by the Polycystic Kidney Research Foundation,

Nashville, TN, June 13-15, 1997; presented talk entitled “Cell Biology 101."

1997 NIDDK Workshop on Polycystic Kidney Disease, September 10-11, 1997, Crystal City, VA; presented overview for session entitled “Cell Biology of PKD” and presented a poster.

American Society of Nephrology, San Antonio, TX, November 2-5, 1997; author on three presentations.

American Society of Nephrology, Philadelphia, PA, October 25-28, 1998; author on three presentations.

American Society for Biochemistry and Molecular Biology, San Francisco, CA, May 16-20, 1999; presented one poster.

Fourth World Congress on Advances in Oncology, and Second International Symposium on Molecular Medicine, Athens, Greece, October 7-9, 1999; presented invited talk entitled “Signal transduction mediated by the polycystic kidney disease-1 protein.”

American Society of Nephrology, Miami Beach, FL, November 4-8, 1999; author on three presentations.

Fifth International Workshop on Polycystic Kidney Disease, Leiden/Noordwijkerhout, Holland, May 11-12, 2000; presented invited talk entitled “The polycystic kidney disease protein, polycystin-1, binds and activates heterotrimeric G-proteins in vitro and in vivo,” and session co-chair.

American Society of Nephrology, Toronto, Canada, October 13-16, 2000; author on three presentations; presented platform talk.

American Society of Nephrology, San Francisco, CA, October 10-17, 2001; author on five poster presentations.

FASEB Summer Research Conference on “Molecular Mechanisms & Therapeutic Insights in Polycystic Kidney Disease,” August 10-15, 2002, Tucson, AZ. Keynote address entitled, “Mechanisms of Cystogenesis” and a regular session talk entitled, “Transcriptional Regulation of the PKD1 Gene.”

American Society of Nephrology, Philadelphia, PA, October 30-November 4, 2002; author on two platform presentations, and three poster presentations.

American Society of Nephrology, San Diego, CA, November 12-17, 2003; author on two poster presentations, and presented invited symposium talk on Nov. 16th entited, “Beta-catenin and the Wnt signaling pathway in PKD.”

International Symposium on Polycystic Kidney Disease; one of 16 international invited speakers; Shanghai, Peoples' Republic of China, May 23-24, 2004; presented invited symposium talk, “Polycystin Signal Transduction.”

American Society of Nephrology, St. Louis, MO, October 27-November 1, 2005. Author on four posters.

Novartis Foundation Discussion Meeting, London, England; January 20-21, 2005; gave invited talk entitled “ Cell Signalling in Polycystic Cells.”

FASEB Summer Research Conference on “New Insights in PKD: Molecular Pathways, Pathogenic Mechanisms, and Translational Applications” at Saxton’s River, VT, August 8-11. Moderated one session.

American Society of Nephrology, Philadelphia, PA, November 10-12, 2005. Moderated

two sessions: “Polycystins: From Cell Signaling to Gene Regulation” and “PKD: Basic Biology.” Author on three posters.

TSC/PKD Workshop “Polycystic Kidney Disease and Tuberous Sclerosis Complex: Building Bridges to the Future” March 9-10, 2006, Chicago, IL. Presented talk “Ca2+ and cAMP signaling in PKD.”

American Society of Nephrology, San Diego, CA, November 14-19, 2006. Moderated one session: “Genetic and Molecular Insights in Renal Cyst Development.” Author on three posters and on one platform presentation (by Brenda Magenheimer).

American Society of Nephrology, San Francisco, CA, November 2-5, 2007. Invited speaker: “Strategies to Inhibit Cyst Formation in ADPKD.” Author on four posters and one platform presentation (by Brenda Magenheimer).

FASEB Summer Research Conference on “Polycystic Kidney Disease: Basic, Translational and Clinical Science” at Snowmass Village, CO, July 27 – August 1. Moderated one session.

American Society of Nephrology, Philadelphia, PA, November 4-9, 2008. Author on three posters and one platform presentation (by Brenda Magenheimer).

2nd International Symposium “Molecular Targets in Renal Disease” Bamberg, Germany, October 17-19, 2008.

Seminars Presented

Nuclear RNA: The Long and Short of It; Department of Biochemistry, University of Kansas, Lawrence, KS; November 16, 1982.

Nuclear RNA: Structure-Function Studies; Department of Biochemistry, University of Kansas Medical Center, Kansas City, KS; April 1, 1983.

Nuclear RNA/RNA Interactions and snRNA Function Analyzed by Psoralen Cross-linking; Department of Biology, University of Puerto Rico, Rio Piedras, PR; September 21, 1983.

In Vivo Secondary Structure and Intermolecular Base-Pairing Interactions Involving the Small Nuclear RNAs Analyzed by Psoralen Cross-linking; Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, MO; October 28, 1983.

Nuclear RNA-RNA Interactions Analyzed by Psoralen Cross-linking; Department of Pharmacology, Toxicology, and Therapeutics, University of Kansas Medical Center, Kansas City, KS; February 7, 1984.

The Use of Psoralen for Cross-linking RNA; Department of Internal Medicine, Division of Dermatology, University of Kansas Medical Center, Kansas City, KS; March 3, 1984.

RNA-RNA Interactions in the HeLa Cell Nucleus Analyzed by Psoralen Cross-linking; Department of Chemistry, Wichita State University, Wichita, KS; October 17, 1984.

In Vivo Secondary Structure and Intermolecular Base-Pairing Interactions Involving Small Nuclear RNAs Analyzed by Psoralen Cross-linking; Department of Human

Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston, TX; December 7, 1984.

The Use of DNA Probes in Diagnosing Infectious Diseases and in Identifying Disease-Causing Mutant Genes; Marion Laboratories, Inc., Kansas City, MO; December 20, 1984.

Interactions Between Small Nuclear RNAs and Precursors of mRNA and rRNA shown by Cross-linking with Psoralen In Vivo; Department of Biochemistry, Kansas State University, Manhattan, KS; March 25, 1985.

Interactions Between Small Nuclear RNAs and Precursors of mRNA and rRNA shown by Cross-linking with Psoralen; School of Basic Life Sciences, University of Missouri-Kansas City, Kansas City, MO; February 13, 1986.

Proto-Oncogene Expression in Polycystic Kidney Disease; Department of Biochemistry, University of Kansas Medical Center, Kansas City, KS; October 2, 1987.

Gene Expression After Toxic Renal Injury and in Polycystic Kidney Disease; Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, KS; November 22, 1988.

Gene Expression After Toxic Renal Injury and in Polycystic Kidney Disease; Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN; April 4, 1989.

Gene Expression After Toxic Renal Injury and in Polycystic Kidney Disease; Department of Physiology and Cell Biology, University of Kansas, Lawrence, KS; November 20, 1989.

Gene Expression Associated with Cell Growth and Differentiation in Polycystic Kidneys; Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS; May 16, 1990.

Gene Expression and Cell Differentiation in Polycystic Kidney Disease; Division of Biology, Kansas State University, Manhattan, KS; February 21, 1992.

Overview of Polycystic Kidney Disease Research at the University of Kansas Medical Center; Sigma Xi presentation, University of Kansas Medical Center, Kansas City, KS; November 19, 1992.

Gene Expression and Abnormalities of Differentiation in Polycystic Kidney Disease; Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS; January 20, 1993.

Polycystic Kidney Disease: A Disease of Many Genes; Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS; October 8, 1993.

Gene Expression and Cell Differentiation in Polycystic Kidney Disease; Worcester Foundation for Experimental Biology, Shrewsbury, MA; December 20, 1993.

Gene Expression and Abnormalities of Differentiation in Polycystic Kidney Disease; Department of Physiology, University of Kansas Medical Center, Kansas City, KS; March 14, 1994.

Cell Differentiation and Injury in Polycystic Kidney Disease; Genetics Society, University of Kansas, Lawrence, KS; April 29, 1994.

Abnormal Transcriptional Cascades in Polycystic Kidneys; Department of Microbiology,

Immunology, and Molecular Genetics, University of Kansas Medical Center, Kansas City, KS; November 17, 1994.

Molecular Genetics of Polycystic Kidney Disease; University of Kansas, Lawrence, KS; November 4, 1998.

A Molecular Analysis of Polycystic Kidney Disease; Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS; December 2, 1999.

Polycystic Kidney Disease: Hints About Protein Function; Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS; November 6, 2000.

Polycystic Kidney Disease: A New Paradigm in Signal Transduction; Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS, February 28, 2002.

Calcium and cAMP Signaling in Polycystic Kidney Disease; Boston University School of Medicine, February 9, 2005.

Calcium and cAMP Signaling in Polycystic Disease; University of Missouri at Kansas City School of Dental Medicine; Oral Biology, December 13, 2006.

The Central Role of cAMP in Polycystic Kidney Disease: Thoughts on Treatments; Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS; December 17, 2007.

Research Update; Kansas City Chapter, PKD Foundation Educational Seminar, March 29, 2008.

Targeting B-Raf in Polycystic Kidney Disease; Interdisciplinary Medical Research Conference at Internal Medicine Grand Rounds, University of Kansas Medical Center, Kansas City, KS; May, 14, 2008.

Cyclic AMP, Calcium, and MAPK Signaling in Polycystic Kidney Disease, Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN; November 18, 2008.

Strategies to Inhibit Cyst Formation in Polycystic Kidney Disease, Division of Nephrology and Hypertension, Mayo Clinic, Rochester, MN; November 18, 2008.

Strategies to Inhibit Cyst Formation in Polycystic Kidney Disease, Division of Nephrology, Medical College of Wisconsin, Milwaukee, WI; February 24, 2009.

Research Update; Kansas City Chapter, PKD Foundation Educational Seminar, March 30, 2009.

Research Update; Kansas City Chapter, PKD Foundation Educational Seminar, March 29, 2010.

Polycystic Kidney Disease: A Calcium Disorder Causing Cellular Transformation. The University of Kansas Cancer Center Seminar Series, February 14, 2012.

Research Update for the PKD Foundation Staff, February 21, 23, 2012.

Research Update for PKDF Volunteers Leadership Conference, Kansas City, MO, March 4, 2012.

Hot Topics, PKD: Pathogenesis to Therapeutics. Laboratory Animal Resources Staff,

Friday, December 14, 2012.

Faculty, Postdoctorals, and Students Mentored/Trained

a. Faculty:

Carolyn A. Rankin, Ph.D. 1986, Research Assistant Professor of Biochemistry and Molecular Biology, (10/1/88-6/30/98) (Grants obtained while associated with my lab: a $10,000 KUCC grant, "Abnormal Proto-oncogene Expression in Cystic Epithelial Cells", a $12,000 NSF grant, "Identification and Characterization of a Murine Transcriptional Regulator Gene", a $20,000 Flossie E. West grant, "Gene Activity During Early Stages of Transformation", and an NIH grant, "The Role of Immortalization Related Genes in PKD (DK44859, $473,812 direct costs, 5 years, 3/15/92 - 2/28/97).

Helen B. Lovell, M.D., 1976, Assistant Professor of Pediatrics, (7/1/89-6/30/90) (Grants obtained while associated with my lab: a $25,000, Department of Pediatrics Telethon grant, "Activated Proto-oncogenes and Cellular Transformation in Polycystic Kidney Disease" and a $16,500 Menorah Medical Center Foundation, Phillip S. Astrowe Trust grant, "Polycystic Kidney Disease Gene Isolation").

Robin L. Maser, Ph.D. 1988, Research Associate Professor of Biochemistry and Molecular Biology, (9/1/95-present) (Grants obtained while associated with my lab: a $25,000 PKRF grant, “Role of Glutathione Peroxidase in Progression of Polycystic Kidney Disease” and a $50,000 AHA grant, “Functional Analysis of a Novel, Potential Modifier Gene of Polycystic Kidney Disease”).

Rafiq Islam, Ph.D., Visiting Faculty (summers 2000-2006), currently Professor and Chair of Chemistry and Physics at Northwest Missouri State University, Maryville, MO. (Grant obtained based on work carried out in my lab: “MEKK1 Downregulates the PKD1 Promoter by a Novel Mechanism” NIH/NIDDK 1R15 DK069897-01 (R. Islam, P.I.) (J. Calvet, 5% effort) Total Direct Costs: $150,000; 03/15/2005 – 02/28/2008 (3 years).

Kathi Glauner, Ph.D., Research Assistant Professor of Biochemistry and Molecular Biology (currently a medical resident in the KU School of Medicine). (Grants obtained while associated with my lab: a $35,000 Lied Endowment Grant, “Tracking Interactions of the Polycystins” 2/1/05-1/31/06 (J. Calvet, Sponsor); a $50,000 Pilot & Feasibility Grant, “Defining Polycystin-1 & -2 Interactions” (a component of the PKD Center grant), awarded but declined because she left the institution to teach for a year at the University of Hawaii, Hilo.

Dubravka Matkovic-Calogovic, Ph.D. Fulbright Senior Scholar (J. Calvet,

Sponsor). (She is Professor of Chemistry at the University of Zagreb, Zagreb, Croatia.) Sept. 2007 to July 2008.

b. Postdoctorals:

Benjamin D. Cowley, Jr., M.D. 1981, Fellow in Nephrology, National Kidney Foundation Research Fellowship, Research Instructor in Medicine, (1985-1987); [National Research Council Fellow, NIH, 1987-1989; currently Professor of Internal Medicine and Director of Nephrology, Univ. of Oklahoma School of Medicine].

Robin L. Maser, Ph.D. 1988, Postdoctoral Fellow (1988-1993), Research Associate (1993-1995) (leave of absence 1/21/90-8/31/90 to serve as an Instructor in the Biology Department, William Jewell College, Liberty, MO); [currently Assistant Professor of Clinical Laboratory Sciences, University of Kansas Medical Center].

Michael A. Harding, Ph.D. 1991, Postdoctoral Fellow (1991-1992); [postdoctoral fellow with Dr. Webster K. Cavenee, Director of the Ludwig Institute, University of California, San Diego (1992-1997); currently a Research Assistant Professor, Department of Urology, University of Virginia]

Xiaoying Hou, M.D. 1990, Postdoctoral Fellow (1993-1994); [went to the Univ. of Alabama, Birmingham and became a postdoctoral fellow in Dr. Lisa Guay-Woodford’s lab].

Michael Sutters, M.D. 1992, Fellow in Nephrology and Internal Medicine Resident (1996-1999); [Assistant Professor of Medicine, Johns Hopkins University School of Medicine. Now in private practice.]

Marianna Rodova, Ph.D. 1983, Research Associate (1998-2002). [currently a Research Associate at KUMC].

Christopher A. Zien, Ph.D. 2000, Postdoctoral Fellow, (2000-2003). [currently employed in industry]

Sanjeev Puri, Ph.D. 1994, Research Associate, (2001-2006). [currently on the faculty in the Department of Biochemistry, Panjab University, Chandigarh, India.]

Ray Hein, Ph.D. 2002, Postdoctoral Fellow (2001-2003). [currently a laboratory manager at Clinical Reference Laboratory, Lenexa, KS].

Stephen C. Parnell, Ph.D. 2001, Postdoctoral Fellow (2006-present) [currently a Research Assistant Professor, KUMC].

Shirin V. Sundar, Ph.D. 2003, Postdoctoral Fellow (2006-2011).

Dar Shah, M.D., Nephrology Fellow (2008-2010).

Sumanth Mulamalla, M.D., Nephrology Fellow (2009-2011).

c. Graduate Students:

Fred L. Smardo, Jr., B.S. 1975, Ph.D. 1985, recipient of the 1985 Philip Newmark Award; Ph.D. Thesis "Molecular Interactions Between Different Classes of RNA from the Nucleus and Cytoplasm of Mammalian Cells", (1982-1985); [attended the University of Kansas School of Medicine, 1985-1989, M.D. 1989; postdoctoral fellow in the National Heart, Lung, and Blood Institute, NIH, 1989-1991; Resident in medicine at the Washington Hospital Center, Washington, DC, 1991-1994; Nephrology fellow at the Barnes Hospital, Washington University School of Medicine, St. Louis, MO, 1994-1996; [currently a nephrologist in private practice].

Robin L. Maser, B.A. 1980, Ph.D. 1988, Biomedical Sciences Scholar Award, National Science Foundation Graduate Fellowship, recipient of the 1986 Joe R. Kimmel Research Award; Ph.D. Thesis "Analysis of the Interaction Between U3 Small Nuclear RNA and Pre-Ribosomal RNA", (1982-1988); [currently Research Associate Professor of Biochemistry and Molecular Biology, University of Kansas Medical Center].

Michael A. Harding, B.S. 1982, Ph.D. (honors) 1991, KU Medical Alumni Distinguished Medical Teaching Fund Scholar Award, Nell A. Kimble Scholarship Award, recipient of the 1990 Philip Newmark Award, recipient of the 1990 Joe R. Kimmel Research Award (1983-1992); [postdoctoral fellow in my lab until 8/92; postdoctoral fellow with Dr. Webster K. Cavenee, Director of the Ludwig Institute, University of California, San Diego (1992-1997); currently a Research Assistant Professor with Dan Theodorescu, Department of Urology, University of Virginia].

Garrett W. Lindemann, B.A. 1985, Ph.D. 1995 (Genetics Program); [postdoctoral fellow at Microgenics: A Boehringer Mannheim Company, Concord, CA, then became a Senior Scientist at Microgenics; currently the owner of a consulting firm, Lindemann Research Consulting, Inc., Big Horn, WY].

Ivy Foo-Hurwitz, B.A. 1986, M.A. 1990, Ph.D. 1999, Kansas Health Foundation Predoctoral Fellowship; [postdoctoral fellow at Children’s Mercy Hospital, Kansas City, MO with Dr. Steve Leder; postdoctoral fellow at the University of Pennsylvania School of Medicine, Philadelphia, PA; postdoctoral fellow at the U. of NM].

Stephen C. Parnell, B.S. 1995, Ph.D. 2001. Kansas Health Foundation Predoctoral Fellowship (1995-2001); [postdoctoral fellow with Dr. Henrik Dohlman, University of North Carolina, Chapel Hill, NC; PKD Foundation Postdoctoral Fellow in the Kidney Institute, KUMC, currently Research Assistant Professor in the Department of Biochemistry and Molecular Biology].

Member, Dissertation Committees: John Ward (Microbiology) (Ph.D. defense, August 20, 1985), Jie Wu (Biochemistry) (Ph.D. defense, June 8, 1988), Lawrence Fernando (Biochemistry) (Ph.D. defense, July 5, 1989), John Lowe (Biochemistry) (Ph.D. defense, November 27, 1989), Gregg Schyuler (Biochemistry) (Ph.D. defense, December 6, 1989), Bernie Beall (Microbiology) (Ph.D. defense, January 16, 1990), T. Trirawatanapong (Biochemistry) (Ph.D. defense, July 6, 1990), Kent Dickeson (Biochemistry) (Ph.D. defense, July 17, 1991), Laurie Hanson (Pharmacology) (Ph.D. defense, April 16, 1992), Larry Dobbs (Biochemistry) (Ph.D. defense, December 15, 1992), Luwen Zhang

(Biochemistry) (Ph.D. defense, March 4, 1993), Sen Xiao (Biochemistry) (Ph.D. defense, April 20, 1993), Ying Peng (Biochemistry) (Ph.D. defense, November 15, 1995), Mini Kapoor (Biochemistry) (Ph.D. defense, October 20, 1995), Bingfang Yan (Pharmacology) (Ph.D. defense, January 19, 1995), Brian Jones (Pharmacology) (Ph.D. defense, April 2, 1996), Luchuan Liang (Biochemistry) (Ph.D. defense, November 30, 1995), Clarissa Davidow (Pharmacology) (Ph.D. defense, May 9, 1996), Denise Signorelli (Biochemistry) (Ph.D. defense, June 11, 1996), Kai Fu (Biochemistry) (Ph.D. defense, April 21, 1997), Shihyun You (Biochemistry) (Ph.D. defense, October 4, 2000), Matt H. Ackermann (Biochemistry) (Ph.D. defense, October 25, 2001) Gerald Call (Physiology) (Ph.D. defense, December 4, 2001), Susanna Harju (Biochemistry) (Ph.D. defense, November 28, 2003), Huimin Jiang (Biochemistry) (Ph.D. defense, April 2, 2004), Yue Xue (Pathology) (Ph.D. defense, June 9, 2004), Ben Weaver (Biochemistry) (Ph.D. defense, April 3, 2009), Subhash Naik (Biochemistry) (Ph.D. defense, November 29, 2012), Sally Salah (Anatomy & Cell Biology), Swathi Iyer (Physiology), Archana Ramon (Physiology), Adam Chazelle (Biochemistry).

Member, M.A. Thesis Committee for Mitch Litwer (Biochemistry) (M.A. thesis defense, December 16, 1982), Kirsten Schartzer (Biochemistry) (M.A. thesis defense, October 22, 1991), Haitao Li (Biochemistry) (M.A. thesis defense, November 27, 1995), Mousheng Xu (Biochemistry) (M.A. thesis defense, January 26, 1996), Yong Tang (Biochemistry) (M.A. thesis defense, November 25, 1996), Ning Deng (Pharmacology) (M.S. thesis defense, September 21, 1999), Michelle David (Biochemistry) (M.S. thesis defense, January 2000), Gregory Bartelma (Biochemistry) (M.S. thesis defense, June 6, 2001), S. Joshua Langmade (Biochemistry) (M.S. thesis defense, November 1, 2001), Anna Nunn (Biochemistry).

Lectures and/or Laboratories Presented in the Following Graduate Courses:

Biochemical Research Techniques: Molecular Genetics

Biochemical Research Techniques: Isotopes and Immunology

Biochemical Research Techniques: Cellular Biochemistry

Theories and Applications of Biochemical Techniques

Advanced Biochemistry

Molecular Genetics

Developmental Biology (Anatomy)

Molecular and Cellular Pharmacology (Pharmacology)

IGPBS Module 3 (Cell and Molecular Biology II)

IGPBS Module 5 (Regulation of Gene Expression and Development)

Carcinogenesis & Cancer Biology (KU Cancer Center)

d. Medical Students:

Kathy R. Gromer, Medical Student Summer Research Traineeship, (summer 1982).

Jonathan G. Wiens, Medical Student Summer Research Traineeship, (summer 1983).

Ellen M. Yetter, employed as student help, (summer, 1990).

Sunny Hwang-Hamilton, employed as student help (spring, 1991).

Charmetra R. Washington, employed as student help (fall 1991 through summer 1992).

Ngoclan T. Nguyen, Medical Student Summer Research Traineeship (with Dr. Rankin), (summer 1992).

Dann J. Fredrickson, Medical Student Summer Research Traineeship (with Dr. Rankin), (summer 1992).

Nils R. Hoernle, Medical Student Summer Research Traineeship, (summer 1996) (First Place - School of Medicine - Student Research Forum)

Joshua Anderson, Medical Student Summer Research Traineeship (summer 2002).

Ikuko Lindsey (Lacheo), Medical Student Summer Research Traineeship (summer 2003).

Regan DeHart, Medical Student Summer Research Traineeship (summer 2004).

Kyle Jansson, M.D./Ph.D. Student Rotation (summer 2007).

Jennifer Truong, Medical School Research Elective (UTMB) (winter 2009).

e. High School and Undergraduate Students:

Carl A. Foster (summer 1983) Biomedical Student Apprentice Program for Disadvantaged High School Students (through office of Affirmative Action, KUMC).

David P. Heitmeyer (recipient of American Society for Microbiology award at 33rd annual Greater Kansas City Science and Engineering Fair; recipient of the Kansas City Star Grand Award, American Society for Microbiology award, Area Medical Council Senior Division 2nd place award, and the United States Navy: Navy Science Award at the 34th Greater Kansas City Science and Engineering Fair; recipient of the General Motors Second Grand Award in Biochemistry, the Patent and Trademark Office award, and the American Chemical Society fourth place award at the 36th International Science and Engineering Fair, Shreveport, LA), (1983-1985 as a high school student; summers of 1986 and 1987 as an undergraduate student); [attended the University of Kansas 1985-1989, B.A. 1989, Harvard University, Ph.D. (chemistry) 1993; currently involved

in software development for course management at Harvard University and teaches in their Division of Continuing Education].

Blake L. Anderson (recipient of American Cancer Society award, United States Air Force Biochemistry award, and Science Pioneers Senior Biological Individual 3rd place award at the 35th Greater Kansas City Science and Engineering Fair), (1985-1986).

Amelia M. Calvet employed as student help (summer 1991).

Krista M. Shipley (spring and summer 1992).

Hilary Commack (summers 2000, 2001).

Terry Peterson (summers and vacations 2001-2007).

Monica Johnson (summers and vacations 2006-present).

Gautham Prakash (science fair and school project 2007-2011).

Rayyan Islam (school project 2008-2010).

Jessica Ebner (volunteer student 2009)

Lauren Brumley (volunteer student 2011)

Tyler Stephenson (Baker University graduate, summer 2012)

Comprehensive Exam and Thesis Committees

Chairman, Ph.D. Thesis Committees for Fred L. Smardo (Ph.D. defense, July 12, 1985), Robin L. Maser (Ph.D. defense, December 8, 1988), Michael A. Harding (Ph.D. defense, December 19, 1991), Garrett W. Lindemann (Ph.D. defense, September 15, 1995), Ivy Foo-Hurwitz (Ph.D. defense, June 4, 1999), Stephen C. Parnell (Ph.D. defense, May 23, 2001).

Member, Ph.D. Thesis Committees for John Ward (Microbiology) (Ph.D. defense, August 20, 1985), Jie Wu (Biochemistry) (Ph.D. defense, June 8, 1988), Lawrence Fernando (Biochemistry) (Ph.D. defense, July 5, 1989), John Lowe (Biochemistry) (Ph.D. defense, November 27, 1989), Gregg Schyuler (Biochemistry) (Ph.D. defense, December 6, 1989), Bernie Beall (Microbiology) (Ph.D. defense, January 16, 1990), T. Trirawatanapong (Biochemistry) (Ph.D. defense, July 6, 1990), Kent Dickeson (Biochemistry) (Ph.D. defense, July 17, 1991), Laurie Hanson (Pharmacology) (Ph.D. defense, April 16, 1992), Larry Dobbs (Biochemistry) (Ph.D. defense, December 15, 1992), Luwen Zhang (Biochemistry) (Ph.D. defense, March 4, 1993), Sen Xiao (Biochemistry) (Ph.D. defense, April 20, 1993), Ying Peng (Biochemistry) (Ph.D. defense, November 15, 1995), Mini Kapoor (Biochemistry) (Ph.D. defense, October 20, 1995), Bingfang Yan (Pharmacology) (Ph.D. defense, January 19, 1995), Brian Jones (Pharmacology) (Ph.D. defense, April 2, 1996), Luchuan Liang (Biochemistry) (Ph.D. defense, November 30, 1995), Clarissa Davidow (Pharmacology) (Ph.D. defense, May 9, 1996), Denise Signorelli (Biochemistry) (Ph.D. defense, June 11, 1996), Kai Fu (Biochemistry) (Ph.D. defense, April 21, 1997), Shihyun You (Biochemistry) (Ph.D. defense, October 4, 2000), Matt H. Ackermann

(Biochemistry) (Ph.D. defense, October 25, 2001) Gerald Call (Physiology) (Ph.D. defense, December 4, 2001), Susanna Harju (Biochemistry) (Ph.D. defense, November 28, 2003), Huimin Jiang (Biochemistry) (Ph.D. defense, April 2, 2004), Yue Xue (Pathology) (Ph.D. defense, June 9, 2004), Ben Weaver (Biochemistry) (Ph.D. defense, April 3, 2009), Subhash Naik (Biochemistry) (Ph.D. defense, November 29, 2012), Sally Salah (Anatomy & Cell Biology), Swathi Iyer (Physiology), Archana Ramon (Physiology), Adam Chazelle (Biochemistry).

Member, M.A. Thesis Committee for Mitch Litwer (Biochemistry) (M.A. thesis defense, December 16, 1982), Kirsten Schartzer (Biochemistry) (M.A. thesis defense, October 22, 1991), Haitao Li (Biochemistry) (M.A. thesis defense, November 27, 1995), Mousheng Xu (Biochemistry) (M.A. thesis defense, January 26, 1996), Yong Tang (Biochemistry) (M.A. thesis defense, November 25, 1996), Ning Deng (Pharmacology) (M.S. thesis defense, September 21, 1999), Michelle David (Biochemistry) (M.S. thesis defense, January 2000), Gregory Bartelma (Biochemistry) (M.S. thesis defense, June 6, 2001), S. Joshua Langmade (Biochemistry) (M.S. thesis defense, November 1, 2001), Anna Nunn (Biochemistry).

Chairman, Oral Comprehensive Committees for Robin Maser, Michael Harding, Susanna Harju, Scott Falke.

Member, Oral Comprehensive Committees for Sue Rockenbach (Microbiology), John Ward (Microbiology), David Dutton (Pharmacology), Larry Dobbs (Biochemistry), Jie Wu (Biochemistry), Michael Arlotto (Pharmacology), Lawrence Fernando (Biochemistry), Thaweesak Trirawatanapong (Biochemistry), Bernie Beall (Microbiology), Laurie Hanson (Pharmacology), Manisha Sahni (Microbiology), Sen Xiao (Biochemistry), Luwen Zhang (Biochemistry), Peter Bullock (Pharmacology), Brian Pfeiffer (Microbiology), Ying Peng (Biochemistry), Mini Kapoor (Biochemistry), Ivy Foo-Hurwitz (Biochemistry), Brian Jones (Pharmacology), Shihyun You (Biochemistry), Stephen Parnell (Biochemistry), Alex Dajkovic (Microbiology), Kellie McQueen (Biochemistry), Christina Newport (Microbiology), Kristina Ollison (Microbiology), Subhash Naik (Biochemistry), Sally Salah (Anatomy & Cell Biology), Swathi Iyer (Physiology), Archana Ramon (Physiology), Adam Chazelle (Biochemistry).

Research Personnel

Leslie M. Meyer, B.A., 1980-1981

Edwin D. Oliver, M.S., 1981-1983

Jill A. Myers, B.S., 1981-1983

Cynthia S. Jacob, B.S., 1984-1985

Shirley G. Crist-Orlando, B.S., 1986-1987

Alice W. Lee, B.S., Jan-Nov 1987

Laurie J. Chadwick, M.S., 1987-1990

Kristine K. Cross, B.S., 1988-1989

Wendy A. Shumate, B.A., May-Dec 1988 (on leave from medical school)

Douglas B. Larsen, 1991-1992 (joint supervision with Dr. Helen Lovell)

Dianne Vassmer, B.S. 1997-1999 (joint supervision with Dr. R. Maser)

Frank Kuhn, M.S. 2000-2001

Dana M. Slaughter, M.S. 2001-2002

Nurjahan Mehzabeen, B.S. 2004-2005 (joint supervision with Dr. Kathi Glauner)

Andreea Chisolita, B.S. 2007

Brenda S. Magenheimer, B.A., 1987-present

Monica Johnson, B.A., 2010-2011.

Lynn Magenheimer, M.A., 2012-present

Lectures and/or Laboratories Presented in the Following Courses

Medical Biochemistry 801, 802 (Course Director 2001-2006)

Biochemical Research Techniques: Molecular Genetics

Biochemical Research Techniques: Isotopes and Immunology

Biochemical Research Techniques: Cellular Biochemistry

Theories and Applications of Biochemical Techniques

Advanced Biochemistry

Molecular Genetics

Developmental Biology (Anatomy)

Molecular and Cellular Pharmacology (Pharmacology)

IGPBS Module 3 (Cell and Molecular Biology II)

IGPBS Module 5 (Regulation of Gene Expression and Development)

Foundations in Medicine

Genetics & Neoplasia (Module Director 2006-present)

Carcinogenesis & Cancer Biology (KU Cancer Center)

Funded Grants as Principal Investigator (or P.I. is associated with my lab)

Patterns of Gene Transcription in HeLa Cells; NIH R01 GM27265/30547;

$155,520 (direct costs); 1980-1983.

Patterns of Gene Transcription in HeLa Cells; BRSG; $15,010; 1981-1982.

RNA-RNA Interactions In Vivo Involving Small Nuclear RNAs and Large Precursors; MACCP; $5,000; 1981-1982.

Nuclear RNA-snRNA Interactions in Mammalian Cells; BRSG; $5,000; 1983-1984.

Nucleolus-Associated Messenger RNA Precursors; Speas Foundation; $19,750; 1984-1985.

Studies on Nucleolar DNA; Fraternal Order of Eagles; $5,000; 1984-1986.

Molecular Genetics of Nucleolar DNA; BRSG; $5,500; 1986.

Cyclic AMP Related Genetic Events in Polycystic Kidney Disease; PKR Foundation; $25,000; 1989-1990.

Molecular Genetics of Nucleolar DNA; NIH R01 GM36547; $181,616 (approx. direct costs); 3 years, Sept. 1, 1986 - Aug. 31, 1989 (with one year extension through Aug 31, 1990).

U3 snRNA-rRNA Interactions; BRSG; $10,000, 1 year, Apr. 1, 1990 - Mar. 31, 1991.

Mechanisms of Cell Proliferation in Polycystic Kidneys; NIH R01 DK37100; $340,922 (est. direct costs); 4 years, Jan. 1, 1987 - Dec. 31, 1990. Competitive renewal: Gene Activity in the Development of Polycystic Kidneys, $549,457 (direct costs); 4 years, Jan. 1, 1991 - Dec. 31, 1994 (with no-cost extension until Dec. 31, 1995).

Role of Glutathione Peroxidase in Progression of Polycystic Kidney Disease; PKRF; $25,000 (direct costs); 1 year, January 1, 1996 - December 31, 1996; Robin L. Maser, P.I.

Gene Activity and Developmental Regulation in Polycystic Kidney Disease; PKR Foundation; $50,000; 18 months, May 1, 1990 - Oct. 31, 1991; with renewal $50,000; 18 months, August 1, 1992 - January 31, 1994; with renewal , $60,000; 18 months, February 1, 1995 - August 31, 1996.

Renal Development and Injury in Polycystic Kidneys; KUMC Research Institute; $25,000; 1 year, July 1, 1995 - June 30, 1996.

Polycystin Function in Normal and PKD Cells; PKR Foundation; $75,000; 18 months, May 1, 1997 - September 30, 1998.

Functional Analysis of a Novel, Potential Modifier Gene of Polycystic Kidney Disease; AHA-KS Affiliate; $49,994 (direct costs); 2 years, July 1, 1996 - June 30, 1998; Robin L. Maser, P.I.

Mouse PKD1 Protein and its Biochemical Interactions; NIH R01 DK51047; $389,349 (direct costs); 4 years, Sept. 29, 1995 - Aug. 31, 1999 (with no-cost extension year until Aug. 31, 2000).

The membrane-associated structure of polycystin-1; Polycystic Kidney Research Foundation (PKRF 98011); $50,000 per year direct costs; January 1, 1999 - December 31, 2001; Robin L. Maser, P.I.

Molecular Mechanisms of Progressive Renal Disorders; NIH P01 DK53763;

$2,736,379 (program project direct costs); 5 years, June 25, 1998 - May 31, 2003; Jared J. Grantham, Program Director; Project 2: Molecular Mechanisms of Polycystin Function; $841,693 (Project 2 direct costs); James P. Calvet, P.I.

The PKD1 Promoter is a Target of the Beta-Catenin/TCF Pathway; Lied Endowed Basic Science Pilot Research Grant, KUMC Research Institute; 2 years, March 21, 2001 - March 20, 2003; $70,000; James P. Calvet, P.I.

Kansas Interdisciplinary Center for PKD Research; NIH P50 DK57301; $4,258,296 (center total costs); 6 years, Sept. 30, 1999 - Aug. 31, 2005; Jared J. Grantham, Center Director; Project 3: Polycystin G-protein signal transduction; $733,405 (Project 3 direct costs); James P. Calvet, P.I.

cAMP-Dependent B-Raf/ERK Activation in a Pkd1 Mouse Model; PKD Foundation; $117,000 (requested direct costs); 2 years, Jan. 1, 2005 - Dec. 31, 2006; James P. Calvet, P.I. (no cost extension year)

B-Raf Inhibition; Deciphera Pharmaceuticals, LLC. $75,000 (direct costs); 1 year (2008-2009), James P. Calvet, P.I.

Kansas Interdisciplinary Center for PKD Research; NIH P50 DK057301; $5,512,500 (total costs); 5 years, Sept. 30, 2005 - Aug. 31, 2010; no-cost extension to Aug. 31, 2011. James P. Calvet, P.I. and Center Director; Project 2: Polycystin-1 Mediated Calcium and cAMP Signaling; $687,500 (Project 2 direct costs); James P. Calvet, P.I.; Administrative Core, $125,000 (Core direct costs); James P. Calvet, P.I.

GERALD M. CARLSON

Professor and Chair

Department of Biochemistry and Molecular Biology

The University of Kansas Medical Center

Mail Stop 3030

3901 Rainbow Boulevard

Kansas City, KS 66160

Phone: (913) 588-7005

Fax: (913) 588-7007

E-Mail: gcarlson@kumc.edu

Personal Information

Born September 26, 1946

Marital Status Married (Susan) with two children: Michael (1970) and Sarah (1977)

Home Address 4501 Holmes Street, Kansas City, MO 64110-1524

Educational Background

1970 - 1975 Ph.D. in Biochemistry (Enzymology), Iowa State University

Department of Biochemistry and Biophysics,

Ames, IA 50011

1964 – 1969 B.S. in Biochemistry, Washington State University

Department of Chemistry, Pullman, WA 99164

Postdoctoral Training

1975 - 1978 Institute for Enzyme Research, University of Wisconsin

Madison, WI 53705

Academic Appointments

2003 - Professor and Chair

Department of Biochemistry and Molecular Biology

University of Kansas Medical Center

1997 - 2002 Marion Merrell Dow/Missouri Professor of Structural Biology and Head

Division of Molecular Biology and Biochemistry

School of Biological Sciences

The University of Missouri – Kansas City

1989 – 1997 Professor and Vice-Chairman

Department of Biochemistry, College of Medicine

The University of Tennessee, Memphis

1986 – 1989 Associate Professor, Department of Biochemistry

College of Medicine, The University of Tennessee, Memphis

1983 – 1986 Associate Professor, Department of Biochemistry

University of Mississippi Medical Center, Jackson, MS

1978 – 1982 Assistant to Associate Professor, Department of Chemistry

University of South Florida, Tampa, FL

Honors and Awards

J. Biol. Chem. corresponding author paper designated as a “Best of 2012” (2013)

Merton F. Utter Memorial Lecture, Case Western Reserve University School of Medicine (2012)

UMKC Trustees’ Faculty Fellow Award (2001)

Marion Merrell Dow/Missouri Endowed Professorship in Structural Biology (1997 - 2002)

Student Government Association of U.T. Memphis Excellence in Teaching Award

College of Graduate Health Sciences (1996)

Excellence in Research Award, U.T. Memphis Chapter of Sigma Xi (1992)

Distinguished Alumnus Award, Department of Biochemistry & Biophysics, Iowa State University (1990)

NIH Research Career Development Award (1983 – 1988)

Outstanding Assistant Professor Award for the College of Natural Sciences

University of South Florida (1982)

Individual NIH-NRSA Postdoctoral Fellowship (1976 – 1977)

Phi Kappa Phi (1973)

NSF Undergraduate Research Fellowship (1968)

Society Memberships

The Biophysical Society (2009)

The Protein Society (1994)

American Chemical Society (1988)

American Society for Biochemistry and Molecular Biology (1982)

Sigma Xi (1977)

President, U.T. Memphis Chapter (1996)

Executive Committee, U.T. Memphis Chapter (1995-1997)

Professional Activities

Editorial Appointments

Editorial Board, Journal of Biochemical and Microbial Technology (2012 - )

Editorial Board, Enzyme Research (2009 - )

Associate Editor, Journal of Biomolecular Techniques (1997 - )

Editorial Board, Journal of Biological Chemistry (1988 - 1993; 1996 - 2000)

Associate Editor, Techniques in Protein Chemistry V-VIII (1994 - 1997), Academic Press

Society Activities

Federation of American Societies for Experimental Biology (FASEB)

Finance Committee (2002 - 2005)

The Protein Society (PS)

Secretary/Treasurer (2002 - 2005)

Executive Council (1999 - 2006)

Publication Committee (2004 - 2005))

Abstract Programming Committee, Chair (1998 - 2001), Member (1997)

Association of Medical and Graduate Departments of Biochemistry (AMGDB)

Past President (2010)

President (2008, 2009)

President Elect (2007)

Board of Directors (2000 - 2002, 2007 - 2010)

National Caucus of Basic Biomedical Science Chairs (2007 - 2008), Member

American Society for Biochemistry and Molecular Biology (ASBMB)

Public Affairs Advisory Committee (2013 - 2016) Program Committee for 2001 Annual Meeting (2000) Miscellaneous National Meetings Organizer, Annual Meeting of Association of Medical and Graduate Departments

of Biochemistry San Juan, Puerto Rico (2008) Organizer, “Symposium on the Mechanisms of Cellular Regulation,” Iowa State

University (2000) Co-organizer, “Biochemical Endocrinology and Signal Transduction Symposium,”

U.T. Memphis (1994)

Grant Review Committees

Canada Foundation for Innovation (Research Hospital Fund Large-scale Institutional Endeavors)

Member Integrative Review Group (2008)

NIH Macromolecular Structure and Function C Study Section

Member (2007 - 2010)

Temporary Member (2005)

53

University of Missouri Research Board (state-wide system) (2001 - 2002)

NIH Physiological Chemistry Study Section

Member (1989 - 1993)

Special Reviewer (1995)

NIH Biomedical Sciences Study Section

Special Reviewer (1994)

NIH Review Panel for Program Project Grant Applications

University of California, San Diego (2007, 2012)

University of Nebraska Medical Center (1997)

Medical College of Ohio (1986)

University of Cincinnati (1986)

NSF Biochemistry Program Advisory Panel

Member (1985 - 1989)

External Advisory Committee

Protein Structure Function COBRE Grant, University of Kansas - Lawrence (2002 - 2003)

Examination Committees

National Board of Medical Examiners, Biochemistry Committee (1985 - 1989)

External Examiner for Dissertation Defenses

University of Calgary, Biochemistry Department (1996)

University of Miami School of Medicine, Biochemistry Department (1982)

Department and Program Review Committees

Wayne State University, Department of Biochemistry and Molecular Biology (2013)

Southern Illinois University School of Medicine, entire program of Department of Microbiology;

Molecular Biology, Microbiology and Biochemistry graduate program (2011)

Visiting Professorships

Pembroke State University, Biology Department

FASEB Visiting Scientist for Minority Institutions Program (1991)

University of South Florida Medical College, Biochemistry Department

54

Series of lectures on carbohydrate metabolism for medical students (1988)

Research Interests

We are studying how communication among subunits of the enzyme phosphorylase kinase (PhK) regulates its enzymatic activity. PhK, which functions in the cascade activation of glycogen breakdown, is a particularly attractive system to study regulatory mechanisms of this type because it is among the largest and most complex enzymes known. Of its 1.3 million Da mass, 90% has a regulatory role. Through allosteric sites on its three regulatory subunits, PhK integrates metabolic (ADP), hormonal (cAMP and Ca2+) and neural (Ca2+) signals, resulting in large changes in its activity. This activity change in response to diverse physiological signals allows for the tight control of glycogenolysis and subsequent energy production, e.g., in skeletal muscle PhK activation by Ca2+ ions couples contraction with energy production to sustain contraction.

We are determining, using a variety of approaches, the mechanisms for how these different signals alter intersubunit interactions and activity of PhK. Two-hybrid genetic screening, protein crosslinking coupled with mass spectrometry, and synthetic peptides are used to identify interacting regions of adjacent subunits. Immunoelectron microscopy with monoclonal antibodies is used to localize regions of subunits within PhK’s overall tetrahedral structure. Immunochemistry and chemical modification are used to identify regions of the protein that are influenced by the allosteric effectors. Finally, site-directed mutagenesis is used to define interacting residues between subunits and to introduce reporter groups. The results from these different experimental approaches coalesce to define the relationships between specific subunit interactions and the control of activity for this important regulatory enzyme of mammalian energy production.

Research Publications 1. Graves, D.J., Carlson, G.M., Skuster, J.R., Parrish, R.F., Carty, T.J. and Tessmer,

G.W. (1975) Pyridoxal phosphate-dependent conformational states of glycogen phosphorylase as probed by interconverting enzymes. J. Biol. Chem. 250, 2254-2258.

2. Carlson, G.M. and Graves, D.J. (1976) Site of action and biphasic effect of neutral salts in the phosphorylase kinase reaction. Biochemistry 15, 4476-4481.

3. Carlson, G.M. and Graves, D.J. (1976) Stimulation of phosphorylase kinase

autophosphorylation by peptide analogs of phosphorylase. J. Biol. Chem. 251, 7480-7486.

4. Colombo, G., Carlson, G.M. and Lardy, H.A. (1978) Phosphoenolpyruvate

carboxykinase (guanosine triphosphate) from rat liver cytosol. Separation of homogenous forms of the enzyme with high and low activity by chromatography on agarose-hexane-guanosine triphosphate. Biochemistry 17, 5321-5329.

55

5. Carlson, G.M., Colombo, G. and Lardy, H.A. (1978) A vicinal dithiol containing an

essential cysteine in phosphoenolpyruvate carboxykinase (guanosine triphosphate) from cytosol of rat liver. Biochemistry 17, 5329-5338.

6. Colombo, G., Carlson, G.M. and Lardy, H.A. (1981) Phosphoenolpyruvate

carboxykinase (guanosine 5'-triphosphate) from rat liver cytosol. Dual cation requirement for the carboxylation reaction. Biochemistry 20, 2749-2757.

7. King, M.M. and Carlson, G.M. (1981) Synergistic effect of Ca2+ and Mg2+ in

promoting an activity of phosphorylase kinase that is insensitive to ethylene glycol bis (β-aminoethyl ether)-N,N'-tetraacetic acid (EGTA). Arch. Biochem. Biophys. 209, 507-523.

8. King, M.M. and Carlson, G.M. (1981) Interaction of phosphorylase kinase with the

2',3'-dialdehyde derivative of adenosine triphosphate. I. Kinetics of inactivation. Biochemistry 20, 4382-4387.

9. King, M.M. and Carlson, G.M. (1981) Interaction of phosphorylase kinase with the

2',3'-dialdehyde derivative of adenosine triphosphate. II. Differential inactivation measured with various protein substrates. Biochemistry 20, 4387-4393.

10. King, M.M. and Carlson, G.M. (1981) Synergistic activation by Ca2+ and Mg2+ as the

primary cause for hysteresis in the phosphorylase kinase reactions. J. Biol. Chem. 256, 11058-11064.

11. King, M.M. and Carlson, G.M. (1982) Affinity labeling of rabbit skeletal muscle

phosphorylase kinase by 5'-(p-fluorosulfonylbenzoyl)adenosine. FEBS Lett. 140, 131-134.

12. King, M.M., Carlson, G.M. and Haley, B.E. (1983) Photoaffinity labeling of the β Subunit of phosphorylase kinase by 8-azidoadenosine 5'-triphosphate and its 2',3'-dialdehyde derivative. J. Biol. Chem. 257, 14058-14068.

13. King, M.M., Fitzgerald, T.J. and Carlson, G.M. (1983) Characterization of initial

autophosphorylation events in rabbit skeletal muscle phosphorylase kinase. J. Biol. Chem. 258, 9925-9930.

14. Cheng, A. and Carlson, G.M. (1983) Utilization of conical equilibrium dialysis cells to

shorten equilibration time. Anal. Biochem. 134, 505-511.

15. Fitzgerald, T.J. and Carlson, G.M. (1984) Activated states of phosphorylase kinase

as detected by the chemical crosslinker 1,5-difluoro-2,4-dinitrobenzene. J. Biol. Chem. 259, 3266-3274.

16. Carlson, G.M. (1984) Precautions when determining kinetically the order of

inactivation of enzymes by functionally irreversible inhibitors. Biochim. Biophys. Acta 789, 347-350.

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17. Cheng, A., Fitzgerald, T.J. and Carlson, G.M. (1985) Adenosine 5'-diphosphate as an allosteric effector of phosphorylase kinase from rabbit skeletal muscle. J. Biol. Chem. 260, 2535-2542.

18. Trempe, M.R., Carlson, G.M., Hainfeld, J.F., Furcinitti, P.S. and Wall, J.S. (1986)

Analyses of phosphorylase kinase by transmission and scanning transmission electron microscopy. J. Biol. Chem. 261, 2882-2889.

19. Trempe, M.R. and Carlson, G.M. (1987) Phosphorylase kinase conformers: detection

by proteases. J. Biol. Chem. 262, 4333-4340.

20. Fitzgerald, T.J., Trempe, M.R. and Carlson, G.M. (1987) Autophosphorylation of the

α subunit of phosphorylase kinase from rabbit skeletal muscle. J. Biol. Chem. 262, 11239-11246.

21. Paudel, H.K. and Carlson, G.M. (1987) Inhibition of the catalytic subunit of

phosphorylase kinase by its α/β subunits. J. Biol. Chem. 262, 11912-11915.

22. Cheng, A., Fitzgerald, T.J., Bhatnagar, D., Roskoski, R., Jr. and Carlson, G.M.

(1988) Allosteric nucleotide specificity of phosphorylase kinase: correlation of binding, conformational transitions, and activation. J. Biol. Chem. 263, 5534-5542.

23. Cheng, A. and Carlson, G.M. (1988) Competition between nucleoside diphosphates

and triphosphates at the catalytic and allosteric sites of phosphorylase kinase. J. Biol. Chem. 263, 5543-5549.

24. Paudel, H.K. and Carlson, G.M. (1988) Renaturation of phosphorylase kinase activity

from sodium dodecyl sulfate-polyacrylamide gels. Arch. Biochem. Biophys. 264, 641-646.

25. Lewis, C.T., Seyer, J.M. and Carlson, G.M. (1989) Cysteine-288: an essential,

hyperreactive thiol of cytosolic phosphoenolpyruvate carboxykinase (GTP). J. Biol. Chem. 264, 27-33.

26. Lewis, C.T., Haley, B.E. and Carlson, G.M. (1989) Formation of an intramolecular

cystine disulfide during the reaction of 8-azido-GTP with cytosolic phosphoenolpyruvate carboxykinase (GTP) causes inactivation without photolabeling. Biochemistry 28, 9248-9255.

27. Paudel, H.K. and Carlson, G.M. (1990) The quaternary structure of phosphorylase

kinase as influenced by low concentrations of urea: evidence for a structural role for calmodulin. Biochem. J. 268, 393-399.

28. Paudel, H.K. and Carlson, G.M. (1990) Functional and structural similarities between

the inhibitory region of troponin I coded by exon VII and the calmodulin-binding regulatory region of the catalytic subunit of phosphorylase kinase. Proc. Natl. Acad. Sci. U.S.A. 87,7285-7289.

57

29. Paudel, H.K. and Carlson, G.M. (1991) The ATPase activity of phosphorylase kinase

is regulated in parallel with its protein kinase activity. J. Biol. Chem. 266, 16524-16529.

30. Farrar, Y.J.K. and Carlson, G.M. (1991) Kinetic characterization of the

calmodulin-activated catalytic subunit of phosphorylase kinase. Biochemistry 30, 10274-10279.

31. Lewis, C.T., Seyer, J.M. and Carlson, G.M. (1992) Photochemical crosslinking of

guanosine 5’-triphosphate to phosphoenolpyruvate carboxykinase (GTP). Bioconjugate Chemistry 3, 160-166.

32. Lewis, C.T., Seyer, J.M., Cassell, R.G. and Carlson, G.M. (1993) Identification of

vicinal thiols of phosphoenolpyruvate carboxykinase (GTP). J. Biol. Chem. 268, 1628-1636.

33. Farrar, Y.J.K., Lukas, T.J., Craig, T.A., Watterson, D.M. and Carlson, G.M. (1993)

Features of calmodulin that are important in the activation of the catalytic subunit of phosphorylase kinase. J. Biol. Chem. 268, 4120-4125.

34. Sanchez, V.E. and Carlson, G.M. (1993) Isolation of an autoinhibitory region from the regulatory β-subunit of phosphorylase kinase. J. Biol. Chem. 268, 17889-17895.

35. Paudel, H.K., Xu, Y.-H., Jarrett, H.W. and Carlson, G.M. (1993) The model

calmodulin-binding peptide melittin inhibits phosphorylase kinase by interacting with its catalytic center. Biochemistry 32, 11865-11872.

36. Bender, P.K., Wang, Z. and Carlson, G.M. (1993) Two exons encode the calmodulin

binding domain in the mouse phosphorylase kinase catalytic subunit gene. Genetic Analysis 10, 99-101.

37. Wilkinson, D.A., Marion, T.N., Tillman, D.M., Norcum, M.T., Hainfeld, J.F., Seyer,

J.M. and Carlson, G.M. (1994) An epitope proximal to the carboxyl terminus of the α-subunit is located near the lobe tips of the phosphorylase kinase hexadecamer. J. Mol. Biol. 235, 974-982.

38. Wilkinson, D.A., Tonin, P., Shanske, S., Lombes, A., Carlson, G.M. and DiMauro, S.

(1994) Clinical and biochemical features of ten adult patients with muscle phosphorylase kinase deficiency. Neurology 44, 461-466.

39. Huang, S., Carlson, G.M. and Cheung, W.Y. (1994) Calmodulin-dependent enzymes

undergo a proton-induced conformational change that is associated with their interactions with calmodulin. J. Biol. Chem. 269, 7631-7638.

40. Norcum, M.T., Wilkinson, D.A., Carlson, M.C., Hainfeld, J.F. and Carlson, G.M.

(1994) Structure of phosphorylase kinase: a three-dimensional model derived from stained and unstained electron micrographs. J. Mol. Biol. 241, 94-102.

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41. Nadeau, O.W. and Carlson, G.M. (1994) Zero-length conformation-dependent cross-

linking of phosphorylase kinase subunits by transglutaminase. J. Biol. Chem. 269, 29670-29676.

42. Xu, Y.-H., Wilkinson, D.A. and Carlson, G.M. (1996) Divalent cations but not other

activators enhance phosphorylase kinase’s affinity for glycogen phosphorylase. Biochemistry 35, 5014-5021.

43. Willkinson, D.A., Norcum, M.T., Fitzgerald, T.J., Marion, T.N., Tillman, D.M. and

Carlson, G.M. (1997) Proximal regions of the catalytic γ and regulatory β subunits on the interior lobe face of phosphorylase kinase are structurally coupled to each other and with enzyme activation. J. Mol. Biol. 265, 319-329.

44. Nadeau, O.W., Sacks, D.B. and Carlson, G.M. (1997) Differential affinity cross-

linking of phosphorylase kinase by the geometric isomers of phenylenedimaleimide. J. Biol. Chem. 272, 29196-26201.

45. Nadeau, O.W., Sacks, D.B. and Carlson, G.M. (1997) The structural effects of

endogenous and exogenous Ca2+/calmodulin on phosphorylase kinase. J. Biol. Chem. 272, 26202-26209.

46. Ayers, N.A., Nadeau, O.W., Read, M.W., Ray, P. and Carlson, G.M. (1998) Effector-sensitive cross-linking of phosphorylase b kinase by the novel crosslinker 4-phenyl-1,2,4-triazoline-3,5-dione. Biochem. J. 331, 137-141.

47. Jeyasingham, M.D. and Carlson, G.M. (1998) Evaluation of phosphoenolpyruvate as

a phosphoryl group donor for phosphoproteins in skeletal muscle. Arch. Biochem. Biophys. 357, 285-292.

48. Nadeau, O.W., Traxler, K.W. and Carlson, G.M. (1998) Zero-length Crosslinking of

the β subunit of phosphorylase kinase to the N-terminal half of its regulatory α subunit. Biochem. Biophys. Res. Commun. 251, 637-641.

49. Wilkinson, D.A., Fitzgerald, T.J., Marion, T.N. and Carlson, G.M. (1999) Mg2+

induces conformational changes in the γ subunit of phosphorylase kinase, whether by itself or as part of the holoenzyme complex. J. Prot. Chem. 18, 157-164.

50. Nadeau, O.W., Traxler, K.W., Fee, L.R., Baldwin, B.A. and Carlson, G.M. (1999)

Activators of phosphorylase kinase alter the cross-linking of its catalytic subunit to the C-terminal one-sixth of its regulatory α subunit. Biochemistry 38, 2551-2559.

51. Xu, Y.-X. and Carlson, G.M. (1999) Structural features contributing to complex

formation between glycogen phosphorylase and phosphorylase kinase. Biochemistry 38, 9562-9569.

52. Ayers, N.A., Wilkinson, D.A., Fitzgerald, T.J. and Carlson, G.M. (1999) Self-

59

association of the alpha subunit of phosphorylase kinase as determined by two-hybrid screening. J. Biol. Chem. 274, 35583-35590.

53. Traxler, K.W., Norcum, M.T., Hainfeld, J.F. and Carlson, G.M. (2001) Direct

visualization of the calmodulin subunit of phosphorylase kinase via subunit exchange and electron microscopy. J. Struct. Biol. 135, 231-238.

54. Nadeau, O.W., Carlson, G.M. and Gogol, E.P. (2002) A Ca2+-dependent global

conformational change in the 3-D structure of phosphorylase kinase obtained from electron microscopy. Structure 10, 23-32.

55. Vénien-Bryan, C., Lowe, E.D., Boisset, N., Traxler, K.W., Johnson, L.N. and Carlson,

G.M. (2002) Three-dimensional structure of phosphorylase kinase at 22 Å resolution and its complex with glycogen phosphorylase b. Structure 10, 33-41.

56. Rice, N.A., Nadeau, O.W., Yang, Q. and Carlson, G.M. (2002) The calmodulin-

binding domain of the catalytic s ubunit of phos phoryla s e kina s e inte ra c inhibitory s ubunit: e vide nce for a Ca2+-sensitive network of quaternary interactions. J. Biol. Chem. 277, 14681-14687.

57. Andreeva, I.E., Rice, N.A. and Carlson, G.M. (2002) The regulatory s ubunit of

phosphorylase kinase may directly participate in the binding of glycogen phosphorylase. Biochemistry (Moscow) 67, 1197-1202.

58. Nadeau, O.W., Gogol, E.P. and Carlson, G.M. (2005) Cryoelectron Microscopy Reveals New Features in the Three-dimensional Structure of Phosphorylase Kinase. Protein Sci. 14, 914-920.

59. Priddy, T.S., MacDonald, B.A., Heller, W.T., Nadeau, O.W., Trewhella, J. and Carlson, G.M. (2005)

Ca2+-Induced Structural Changes in Phosphorylase Kinase Detected by Small-angle X-ray Scattering. Protein Sci. 14, 1039-1048. (Selected for cover)

60. Hilder, T.L., Carlson, G.M., Haystead, T.A.J., Krebs, E.G. and Graves, L.M. (2005) Caspase-3

Dependent Cleavage and Activation of Skeletal Muscle Phosphorylase b Kinase. Molec. Cell. Biochem. 275, 233-242.

61. Archila, S., King, M.A., Carlson, G.M. and Rice, N.A. (2006) The Cytoskeletal Organizing Protein Cdc42-interacting Protein 4 Associates with Phosphorylase Kinase in Skeletal Muscle. Biochem. Biophys. Res. Commun. 345, 1592-1599.

62. Nadeau, O.W., Anderson, D.W., Yang, Q., Artigues, A., Paschall, J.E., Wyckoff, G.J., McClintock, J.L. and Carlson, G.M. (2007) Evidence for the Location of the Allosteric Activation Switch in the Multisubunit Phosphorylase Kinase Complex from Mass

60

Spectrometric Identification of Chemically Crosslinked Peptides. J. Mol. Biol. 365, 1429-1445.

63. Priddy, T.S., MIddaugh, C.R. and Carlson, G.M. (2007) Electrostatic Changes in Phosphorylase Kinase Induced by Its Obligatory Allosteric Activator Ca2+. Protein Sci. 16, 517-527.

64. Priddy, T.S., Price, E.S., Johnson, C.K. and Carlson, G.M. (2007) Single Molecule Analyses of the Conformational Substates of Calmodulin Bound to the Phosphorylase Kinase Complex. Protein Sci. 16, 1017-1023.

65. Stiffin, R.S., Sullivan, S.M., Carlson, G.M. and Holyoak, T. (2008) Differential Inhibition of Cytosolic PEPCK by Substrate Analogues. Kinetic and Structural Characterization of Inhibitor Recognition. Biochemistry 47, 2099-2109.

66. Jeyasingham, M.D., Artigues, A., Nadeau, O.W. and Carlson, G.M. (2008) Structural Evidence for Co-evolution of the Regulation of Contraction and Energy Production in Skeletal Muscle. J. Mol. Biol. 377, 623-629.

67. Nadeau, O.W., Wyckoff, G.J., Paschall, J.E., Artigues, A., Sage, J., Villar, M.T. and Carlson, G.M. (2008) CrossSearch, a User-friendly Search Engine for Detecting Chemically Cross-linked Peptides in Conjugated Proteins. Mol. Cell. Proteomics 7, 739-749.

68. Boulatnikov, I.G., Nadeau, O.W., Daniels, P.J., Sage, J.M., Jeyasingham, M.D., Villar, M.T., Artigues, A. and Carlson, G.M. (2008) The Regulatory S ubunit of Phosphorylase Kinase Interacts with Glyceraldehyde-3-phosphate Dehydrogenase. Biochemistry 47, 7228-7236.

69. Liu, W., Priddy, T.S. and Carlson, G.M. (2008) Physicochemical Changes in Phosphorylase Kinase Associated with Its Activation. Protein Sci. 17, 2111-2119.

70. Boulatnikov, I.G., Peters, J.L., Nadeau, O.W., Sage, J.M., Daniels, P.J., Kumar, P., Walsh, D.A. and Carlson, G.M. (2009) Expressed Phosphorylase b Kinase and Its S ubcomple x a s Re gula tory Mode ls for the Ra bbit S ke le ta l Mus cle Holoenzyme. Biochemistry 48, 10183-10191.

71. Nadeau, O.W., Liu, W., Boulatnikov, I.G., Sage, J.M., Peters, J.L. and Carlson, G.M. (2010) The Glucoamylase Inhibitor Acarbose Is a Direct Activator of Phosphorylase Kinase. Biochemistry 49, 6505-6507.

72. Nadeau, O.W., Lane, L.A., Xu, D., Sage, J., Priddy, T.S., Artigues, A., Villar, M.T., Yang, Q., Robinson, C.V., Zhang, Y., and Carlson, G.M. (2012) The Structure and

61

Location of the Regulatory S ubunits in the ()4 Phosphorylase Kinase Complex. J. Biol. Chem. 287, 36651-36661.

[At publication selected as a Paper of the Week for being “in the top 2 percent

of manuscripts [reviewed] in a year in significance and overall importance”: J. Biol. Chem. 287, 36662 (2012)]

[In January 2013 selected as one of “only 22 papers – out of more than 4,000

published last year – to receive the designation ‘JBC’s Best of 2012’ (http://www.jbc.org/site/bestoftheyear/)”]

73. Lane, L.A., Nadeau, O.W., Carlson, G.M. and Robinson, C.V. (2012) Mass Spectrometry Reveals Differences in Stability and Subunit Interactions between Activated and Nonactivated Conformers of the ( )4 Phosphorylase Kinase Complex. Mol. Cell. Proteomics 11, 1768-1776.

74. Liu, W., Nadeau, O.W. and Carlson, G.M. (2013) Physicochemical Changes in Phosphorylase Kinase Induced by the Cationic Activator Mg2+. Protein Sci. 22, 444-454.

Reviews and Book Chapters 1. Carlson, G.M., Tabatabai, L.B. and Graves, D.J. (1976) The use of an alternative

substrate as a model system for the study of phosphorylase kinase. Metabolic Interconversion of Enzymes (S. Shaltiel, Ed.), pp. 50-59, Springer-Verlag, Heidelberg.

2. Carlson, G.M., Bechtel, P.J. and Graves, D.J. (1979) Chemical and regulatory

properties of phosphorylase kinase and cAMP-dependent protein kinase. Advances in Enzymology 50, 41-115.

3. Carlson, G.M. and Nadeau, O.W. (1999) Bifunctional crosslinking reagents. The

Encyclopedia of Molecular Biology (T.E. Creighton, Ed.), Vol. 1, pp. 279-281, Wiley, New York.

4. Carlson, G.M. and Nadeau, O.W. (1999) Crosslinking. The Encyclopedia of

Molecular Biology (T.E. Creighton, Ed.), Vol. 1, pp. 582-584, Wiley, New York.

5. Carlson, G.M. and Nadeau, O.W. (1999) Enzyme immobilization and conjugation.

The Encyclopedia of Molecular Biology (T.E. Creighton, Ed.), Vol. 2, p. 829, Wiley, New York.

6. Carlson, G.M. and Nadeau, O.W. (1999) Glutaraldehyde. The Encyclopedia of

Molecular Biology (T.E. Creighton, Ed.), Vol. 2, pp. 1019-1020, Wiley, New York.

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7. Rice, N.A. and Carlson, G.M. (2001) Phosphorylase kinase. The Encyclopedia of

Molecular Medicine (T.E. Creighton, Ed.), Vol. 4, pp. 2487-2490, Wiley, New York. 8. Nadeau, O.W. and Carlson, G.M. (2002) Chemical Cross-linking in Studying Protein-

protein Interactions. Protein-Protein Interactions (E.A. Golemis, Ed.), pp. 75-91, Cold Spring Harbor Laboratory Press, New York.

9. Nadeau, O.W. and Carlson, G.M. (2005) Protein Interactions Captured by Chemical Cross-linking.

Protein-protein Interactions: A Molecular Cloning Manual (E.A. Golemis, Ed., 2nd ed.), pp. 105-127, Cold Spring Harbor Laboratory Press, New York.

10. Carlson, G.M. and Holyoak, T. (2009) Structural Insights into the Mechanism of Phosphoenolpyruvate Carboxykinase Catalysis. J. Biol. Chem. 284, 27037-27041. (Invited minireview)

11. Carlson, G.M. (2010) How I Became a Biochemist. IUBMB Life 62, 158-161. (Invited memoir)

12. Nadeau, O.W. and Carlson, G.M. (2012) Methods for Detecting Structural Changes in Large Protein

Complexes. Methods in Molecular Biology, Vol. 796 (Allostery: Methods and Protocols; A.W. Fenton,

Ed.), pp. 117-132, Humana Press, Totowa, NJ. Extramural Research Support

Below are listed only those grants on which GMC was Principal Investigator. The dollar amounts listed are for the actual awarded direct costs only. Additional grants not listed include several shared instrumentation grants, international travel grants, including a NATO award, administrative supplements from NIH, and competitive intramural grants from state and local sources.

National Institutes of Health

Phosphorylase kinase: role of autophosphorylation

(R01 AM26591/changed to AM32953 upon moving; years 1-2 of 32953)

1980 – 1983, $147,257 Direct Costs

Structure of phosphorylase kinase (R01 AM32953; years 3-7)

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1983 – 1988, $362,386 Direct Costs

Activation and conformation of phosphorylase kinase (K04 AM01234)

Research Career Development Award

1983 – 1988, $219,831 Direct Costs

Subunit interactions of phosphorylase kinase (R01 DK32953; years 8-12)

1989 – 1994, $654,292 Direct Costs

Subunit interactions of phosphorylase kinase (R01 DK32953; years 13-17)

1994 – 1999, $797,487 Direct Costs

Subunit interactions of phosphorylase kinase (R01 DK32953; years 18-21)

2000 – 2005, $915,969 Direct Costs

Subunit interactions of phosphorylase kinase (R01 DK32953; years 22-25)

2005 – 2009, $1,042,554 Direct Costs

Proteomics to Screen for a Protein Kinase that Uses PEP (R21 DK072393; Years 1-2)

2005 – 2007, $275,000 Direct Costs

Subunit interactions of phosphorylase kinase (R56 DK32953; year 26)

2009 – 2010, $333,333 Direct Costs

Subunit interactions of phosphorylase kinase (R01 DK32953; years 27-30)

2010 – 2014, $1,278,906 Direct Costs

National Science Foundation

Mapping the active site of phosphoenolpyruvate carboxykinase (DMB-850311)

1986 – 1990, $134,936 Direct Costs (Did not attempt to renew)

Muscular Dystrophy Association

Autophosphorylation of phosphorylase kinase from skeletal muscle

64

1979 – 1981, $50,043 Direct Costs

Interaction of phosphorylase kinase with adenine nucleotides and calcium and magnesium ions

1981 – 1983, $26,389 Direct Costs (Did not attempt to renew) Fellows and Graduate Students Trained Postdoctoral Associates

Kincaid, Margaret (2011 - )

Funke, Todd (2011 - 2012)

Peters, Jennifer (2007 - 2011)

Liu, Weiya (2006 - 2012)

Boulatnikov, Igor (2005 - 2011)

Falke, Scott (2004)

Daniels, Patrick (2003 - 2007)

Nadeau, Owen W. (1991 - 1997; 1999 - , Research Assistant Professor)

Jeyasingham, Marina D. (1987 - 1991; 1999 - 2009, Research Assistant Professor)

Traxler, Kenneth W. (1994 - 1997; Summers 1998 - 2003)

Yang, Qing (2000 - 2001)

Andreeva, Iraida E. (1999 - 2000)

Huang, Shengli (1997 - 1999)

Fee, Lanette (1995 - 1997)

Farrar, Young J.K. (1987-1989)

Paudel, Hemant K. (1985 - 1988, 1989 - 1991)

Norcum, Mona R. (Trempe) (1984 - 1986) Graduate Students

Thompson, Jackie (2013 - )

Rimmer, Mary Ashley (2012 - )

Priddy, Timothy S. Ph.D. in 2006

Anderson, David M.S. in 2002

Rice, Nancy A. (Ayers) Ph.D. in 1999

Xu, Yihong Ph.D. in 1994

65

Stiffin, Rose M. Ph.D. in 1994

Wilkinson, Deborah A. Ph.D. in 1993

Sanchez, Veronica E. Ph.D. in 1993

Lewis, Cristina T. Ph.D. in 1989

Cheng, Alexander Ph.D. in 1986

Herrera, Julio E. M.S. in 1986

Foster, Stephen M. M.S. in 1985

Fitzgerald, Thomas J. Ph.D. in 1985

King, Marita M. Ph.D. in 1981

Invited Scientific Presentations

Invited Presentations at International, National and Regional Meetings

"New Frontiers in Enzymology: Enzyme Complexes and Regulation," Plenary lecture,

Occidental Grand Xcaret Resort, Mexico (2013)

“Enzymes, Coenzymes & Metabolic Pathways” Gordon Conference,

Waterville Valley, NH (2012)

“8th International Symposium on Mass Spectrometry in the Health and Life Sciences:

Molecular and Cellular Proteomics,” San Francisco, CA (2007)

“Strategies for Building Multi-scale Structural Models of Cell Signaling Complexes,”

Los Alamos/UCSD Workshop, San Diego, CA (2001)

“Symposium on the Mechanisms of Cellular Regulation,” Ames, IA, (2000)

“North Dakota EPSCoR Conference on Protein-Protein Interactions,” Grand Forks (1998)

Annual Conference of the Association of Medical and Graduate Departments of Biochemistry, Belize City, Belize (1998)

ASBMB Fall Symposium on “Structure and Function of Protein Kinases and Phosphatases,” Keystone, CO (1992)

“12th Enzyme Mechanisms Conference,” San Diego (1991) Talk summarized in Bioorganic Chemistry 19, 190-224 (1991)

“Techniques, Advances and Utilization of Base Modified Nucleotide Photoaffinity Probes,” Subgroup Meeting at ASBMB/ASCB Joint Meeting, San Francisco (1989)

FASEB Summer Research Conference on “Protein Kinases,” Saxtons River, VT (1985)

66

“Between the Hope and the Cure,” Florida West Coast Chapter of Muscular Dystrophy Association, Sarasota (1980)

“Report from the Research Front,” Atlanta Chapter of Muscular Dystrophy Association, Atlanta (1980)

Invited Seminars at Universities, Institutes and Companies (Since 1990, Excluding Job Interviews)

2012 Case Western Reserve University, Department of Biochemistry

2010 Wichita State University, Departments of Chemistry and Biological Sciences

Missouri State University, Department of Chemistry

2009 University of Missouri – Kansas City, School of Biological Sciences,

Vanderbilt University, Department of Molecular Physiology and Biophysics

2008 Oklahoma State University, Department of Biochemistry and Molecular Biology University of California, Santa Barbara, Department of Chemistry

2006 University of Massachusetts Medical School, Dept. Biochemistry & Molecular Pharmacology

2005 Western Kentucky University, Department of Biology

University of Missouri – Columbia, Department of Biochemistry

2004 University of Missouri – Kansas City, School of Pharmacy

University of Utah, Department of Chemistry

Stowers Institute for Medical Research, Kansas City, MO

2003 University of South Florida, Department of Biochemistry and Molecular Biology

University of Kentucky, Department of Chemistry

2002 Indiana University School of Medicine, Department of Biochemistry and Molecular Biology

Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Montreal, Quebec

2001 University of Kansas, Department of Molecular Biosciences, Lawrence, KS 1999 Ruhr-Universitat Bochum, Institut fur Physiologische Chemie, Bochum,

Germany

1997 University of North Carolina (Chapel Hill), Pharmacology Department

Hexos, Inc., Bothell, WA

University of Kansas Medical Center, Biochemistry Department

67

Kansas State University, Department of Biochemistry 1996 URA CNRS (Tours), France 1995 University of South Florida, Department of Chemistry

1994 University of Mississippi Medical Center, Biochemistry Department

Virginia Polytechnic Institute, Biochemistry and Nutrition Department

Louisiana State University Medical Ctr (Shreveport), Biochemistry & Molecular Biology Dept. 1993 Indiana University School of Medicine, Department of Biochemistry and

Molecular Biology

1992 Baylor College of Medicine, Biochemistry Department

University of Wyoming, Molecular Biology Department

University of Arkansas for Medical Sciences, Biochemistry and Molecular Biology Department

1991 Memphis State University, Biology Department

Mississippi State University, Biochemistry and Molecular Biology Department

W. Alton Jones Cell Science Center, Lake Placid

University of Vermont, Department of Biochemistry

1990 Meharry Medical College, Biochemistry Department

Iowa State University, Biochemistry and Biophysics Department

Ohio State University, Biochemistry Program

University of Alberta, Biochemistry Department

University of Calgary, Cell Regulation Department

68

CURRICULUM VITAE

Name: Joan W. Conaway, Ph.D.

Born: May 8, 1956

New York, New York

Address: Stowers Institute for Medical Research

1000 E. 50th Street

Kansas City, MO 64110

Tel: 816-926-4091

jlc@stowers.org

Education:

1974-1978 A.B., Bryn Mawr College, in Chemistry and in Biology with honors from Haverford College

1979-1987 Ph.D., in Cell Biology, Stanford University School of Medicine, with Dr. Roger Kornberg

Post-Graduate Training:

1987-1988 DNAX Institute Research Fellow, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA.

Professional Appointments:

2001- Investigator, Stowers Institute for Medical Research

2001- Professor (Affiliate), Department of Biochemistry and Molecular Biology, University of Kansas Medical Center

2000 Interim Head, Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation

1998-2006 Adjunct Professor, Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center

69

1997-2001 Associate Investigator, Howard Hughes Medical Institute

1996-2001 Member, Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK.

1993-1996 Associate Member, Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK.

1991-1998 Adjunct Assistant Professor, Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center

1989-1993 Assistant Member, Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK.

1988-1989 Research Associate and Lecturer, Clayton Foundation Biochemical Institute, Department of Chemistry, University of Texas, Austin, TX.

Academic Activities:

Thesis Committees Biochemistry and Molecular Biology, University of Kansas Medical Center -

Matt Goering

Ray Camahort

Zhen Zhang (current)

Jackie Thompson (current)

Other KUMC departments

Shachi Bhatt

Kendall Smith

Jason Ross

Wen Tang

Evan Janzen

Todd Bradley

Honors:

1991 Edward L. and Thelma Gaylord Award for Scientific Excellence

1997 ASBMB-Amgen Award

2001 Burroughs-Wellcome Visiting Professorship, Saint Louis University School of Medicine

2002 Elected Fellow, American Academy of Arts and Sciences

2005 Helen Nelson Distinguished Investigatorship

Advisory Boards

70

Chairperson, Board of Scientific Counselors - Basic Science, National Cancer Institute, Bethesda, MD,

September 2011 - July 2015

Board of Scientific Counselors - Basic Science, National Cancer Institute, Bethesda, MD,

September 2010 - July 2015

Scientific Advisory Board, Institute for Genetics and Molecular and Cellular Biology (IGBMC)

Strasbourg, France, September 2010 -

Editorial Boards and Review Committees

Editorial Committee, Annual Review of Biochemistry, January 2007- present

Associate Editor, Journal of Biological Chemistry, September 1999- present

Member, NIH Molecular Biology Study Section, February 1994- June 1998

Ad Hoc Member, NIH Molecular Biology Study Section, February 1993

Editorial Board Member, Journal of Biological Chemistry, July 1993 - July 1998

Other Professional Activities:

American Society for Biochemistry and Molecular Biology Meetings Committee, 2002-2004; 2006-2009 (co-chair, small meetings subcommittee); 2009-2012 (committee chair).

Co-chair, Program Committee for the 2009 National Meeting of the American Society for Biochemistry and Molecular Biology.

Programming Consultant, 2006 Keystone Symposia Series

Co-organizer, 2005 and 2007 Cold Spring Harbor Ubiquitin Family Meeting

Council, American Society of Biochemistry and Molecular Biology, July 2004-July 2007.

Co-organizer, 2003 and 2005 Cold Spring Harbor Meeting on Mechanisms of Eukaryotic Transcription.

Co-Chair, Program Committee for the 2002 National Meeting of the American Society for Biochemistry and Molecular Biology, New Orleans, LA.

Chair, Selection Committee, FASEB Excellence in Science Award, 2001

Co-organizer, 2001 Keystone Symposium “Mechanisms of Eukaryotic Transcriptional Regulation.”

Member, Program Committee for the 2001 National Meeting of the American Society for Biochemistry and Molecular Biology, Orlando, FL.

Co-organizer, 1999 ASBMB Fall Symposium “Mechanism and Regulation of Transcription by RNA Polymerase II”

71

Member, Nominations Committee, American Society for Biochemistry and Molecular

Biology, 1999-2002

Member, Selection Committee, FASEB Excellence in Science Award, 1998 - 2000

Session chair, 1996 Gordon Conference on Molecular Genetics

Session chair, 1993 Gordon Conference on Nucleic Acids

Co-organizer, 1992 Keystone Symposium "Fundamental Mechanisms of Transcription"

Session chair, 1992 Gordon Conference on Nuclear Proteins, Chromatin Structure, and Gene Expression

BIBLIOGRAPHY

1. Conaway, J. W., Bond, M. W., and Conaway, R. C. (1987) An RNA polymerase II

transcription system from rat liver: Purification of an essential component. J. Biol. Chem. 262, 8293-8297.

2. Otsuka, T., Miyatake, S., Yokota, T., Conaway, J., Conaway, R., Arai, N., Lee, F. and

Arai, K. (1987) Organization of the chromosomal genes for interleukin-3 and granulocyte-macrophage colony stimulating factor and their expression in activated T cells. Lymphokines 13, 261-273.

3. Yokota, T., Miyatake, S., Hagiwara, H., Mosmann, T., Conaway, J., Conaway, R.,

Miyajima, A., Takebe, Y., Arai, N., Lee, F. and Arai, K. (1988) Isolation and characterization of the mouse interleukin-3 gene and its expression in activated T cells. Lymphokines 15, 393-408.

4. Conaway, R.C. and Conaway, J.W. (1988) ATP activates transcription initiation from

promoters by RNA polymerase II in a reversible step prior to RNA synthesis. J. Biol. Chem. 263, 2962-2968.

5. Conaway, J.W. and Conaway, R.C. (1989) A multisubunit transcription factor essential

for accurate initiation by RNA polymerase II. J. Biol. Chem. 264, 2357-2362.

6. Conaway, J.W., Conaway, R.C. and Muramatsu, M. Signal transduction and transcription

regulation, in Cell Technology. Tokyo: Shujunshu, 1989.

7. Conaway, R.C. and Conaway, J.W. (1989) An RNA polymerase II transcription factor has

an associated DNA-dependent ATPase (dATPase) activity strongly stimulated by the TATA region of promoters. Proc. Natl. Acad. Sci. USA 86, 7356-7360.

8. Conaway, J.W., Reines, D. and Conaway, R.C. (1990) Transcription initiated by RNA

polymerase II and purified transcription factors from liver: Cooperative action of transcription factors τ and ε in initial complex formation. J. Biol. Chem., 265, 7552-7558.

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9. Conaway, R.C. and Conaway, J.W. (1990) Transcription initiated by RNA polymerase II

and purified transcription factors from liver: Transcription factors α, βγ, and δ promote formation of intermediates in assembly of the functional preinitiation complex. J. Biol. Chem., 265, 7559-7563.

10. Conaway, J.W., Travis, E. and Conaway, R.C. (1990) Transcription initiated by RNA

polymerase II and purified transcription factors from liver: A complex set of promoter sequences governs formation of the initial complex. J. Biol. Chem., 265, 7564-7569.

11. Conaway, J.W. and Conaway, R.C. (1990) An RNA polymerase II transcription factor

shares functional properties with Escherichia coli σ70. Science 248, 1550-1553.

12. Conaway, J.W., Hanley, J.P., Garrett, K.P. and Conaway, R.C. (1991) Transcription

initiated by RNA polymerase II and transcription factors from liver: Structure and action of transcription factors ε and τ. J. Biol. Chem., 12, 7804-7811.

13. Conaway, R.C., Garrett, K.P., Hanley, J.P. and Conaway, J.W. (1991) Mechanism of

promoter selection by RNA polymerase II. Mammalian transcription factors α and βγ promote entry of polymerase into the preinitiation complex. Proc. Natl. Acad. Sci., 88, 6205-6209.

14. Conaway, J.W. and Conaway, R.C. (1991) Initiation of eukaryotic messenger RNA

synthesis. (mini-review) J. Biol. Chem., 266, 17721-17724.

15. Tsuboi, A., Conger, K., Garrett, K.P. Conaway, R.C., Conaway, J.W., and Arai, N. (1992).

RNA polymerase II initiation factor α from rat liver is almost identical to human TFIIB. Nucl. Acids Res., 20, 3250.

16. Conaway, J.W., Bradsher, J.N. and Conaway, R.C. (1992). Mechanism of assembly of

the RNA polymerase II preinitiation complex: Transcription factors δ and ε promote stable binding of the transcription apparatus to the initiator element. J. Biol. Chem., 267, 10142-10148.

17. Conaway, R.C., Bradsher, J.N. and Conaway, J.W. (1992). Mechanism of assembly of

the RNA polymerase II preinitiation complex: Evidence for a functional interaction of the carboxyl terminal domain of RNA polymerase II and a high molecular mass form of the TATA factor. J. Biol. Chem., 267, 8464-8467.

18. Serizawa, H., Conaway, R. C. and Conaway, J.W. (1992). A carboxyl-terminal-domain

kinase associated with RNA polymerase II transcription factor δ from rat liver. Proc. Natl. Acad. Sci. USA, 89, 7476-7480.

19. Garrett, K.P., Serizawa, H., Hanley, J.P., Bradsher, J.N., Tsuboi, A., Arai, N., Yokota, T.,

Arai, K., Conaway, R.C., Conaway, J.W. (1992) The C-terminus of RAP30 is similar in sequence to region 4 of bacterial sigma factors and is required for function. J. Biol. Chem. 267, 23942-23949.

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20. Conaway, R.C. and Conaway, J.W. (1993) General initiation factors for RNA polymerase

II. Ann. Rev. Biochem. 62, 161-190.

21. Serizawa, H., Conaway, J.W., and Conaway, R.C. (1993) Phosphorylation of C-terminal

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120. Sato, S., Tomomori-Sato, C., Parmely, T.J., Florens, L., Zybailov, B., Swanson, S.K., Banks, C.A.S., Jin, J., Cai, Y., Washburn, M.P., Conaway, J.W., and Conaway, R.C. (2004) A set of consensus mammalian Mediator subunits identified by multidimensional protein identification technology. Mol. Cell 14, 685-691.

121. Bourbon, H.M., Aguilera, A., Ansari, A.Z., Asturias, F.J., Berk, A.J., Bjorklund, S., Blackwell, T.K., Borggrefe,T., Carey, M., Carlson, M., Conaway, J.W., Conaway, R.C., Emmons, S.W., Fondell, J.D., Freedman, L.P., Fukaswa, T., Gustafsson, C.M., Han, M., He, X., Herman, P.K., Hinnebusch, A., Holmberg, S., Holstege, F.C., Jaehning, J.A., Kim, Y.J., Kuras, L., Leutz, A., Lis, J.T., Meisterernst, M., Naar, A.M., Nasmyth, K., Parvin, J.D., Ptashne, M., Reinberg, D., Ronne, H., Sadowski, I., Sakurai, H., Sipiczki, M., Sternberg, P.W., Stillman, D.J., Strich, R., Struhl, K., Svejstrup, J.Q., Tuck, S., Winston, F., Roeder, R.G., Kornberg, R.D. (2004) A unified nomenclature for protein subunits of Mediator complexes linking transcriptional regulators to RNA polymerase II. (2004) Mol. Cell 14, 553-557.

122. Yan, Q., Kamura, T., Cai, Y., Jin, J., Ivan, M., Conaway, J.W., and Conaway, R.C. (2004) Identification of Elongin C and Skp1 Sequences that Determine Cullin Selection, J. Biol. Chem. 279, 43019-43026, First published on-line July 27, 2004.

123. Gerber, M., Eissenberg, J.C., Kong, S., Tenney, K., Conaway, J.W., Conaway, R.C., Shilatifard, A., (2004) In vivo requirement of the RNA polymerase II elongation factor Elongin A for proper gene expression and development. Mol. Cell Biol. 24 9911-9919.

124. Blanchette, P., Cheng, C.Y., Yan, Q., Ketner, G., Ornelles, D.A., Dobner, T., Conaway, R.C., Conaway, J.W., and Branton, P.E. (2004) Both BC-box motifs of adenovirus protein E4orf6 are required to efficiently assemble an E3 ligase complex that degrades p53. Mol. Cell. Biol. 24, 9619-9629.

125. Kamura T.; Maenaka, K.; Kotoshiba, S.; Matsumoto, M.; Kohda, D.; Conaway, R.C.; Conaway, J.W.; Nakayama, K.I.; (2004) VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases. Genes Dev. 18 3055-3065.

126. Heuze, M.L.; Guibal, F.C.; Banks, C.A.; Conaway, J.W.; Conaway, R.C.; Cayre, Y.E.; Benecke, A.; Lutz, P.G.; (2005) ASB2 is an Elongin BC-interacting protein that can assemble with Cullin 5 and Rbx1 to reconstitute an E3 ubiquitin ligase complex. J.Biol.Chem. 280 5468-5474.

127. Gerber M.; Tenney, K.; Conaway, J.W.; Conaway, R.C. Eissenberg, J.C.; Shilatifard, A.; (2005) Regulation of heat shock gene expression by RNA polymerase II elongation factor, Elongin A. J.Biol.Chem. 280 4017-4020.

128. Conaway, J.W., Florens, L., Sato, S., Tomomori-Sato, C., Parmely, T.J., Yao, T., Swanson, S.K., Banks, C.A.S., Washburn, M.P., and Conaway, R.C. (2005) The Mammalian Mediator Complex. FEBS Lett. 579, 904-908 (special issue, Proceedings of the 130th Nobel Symposium on Molecular Mechanisms in Biological Regulation).

83

129. Cai, Y., Jin, J., Florens, L., Swanson, S.K., Kusch, T., Li, B., Workman, J.L., Washburn, M.P., Conaway, R.C., Conaway, J.W. (2005) The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 histone acetyltransferase and SRCAP complexes. J. Biol. Chem., 280 13665- 13670. First published on-line Apr. 8, 2005.

130. Conaway, R.C.; Sato, S.; Tomomori-Sato, C.; Yao, T.; Conaway, J.W.; (2005) The mammalian Mediator complex and its role in transcriptional regulation. Trends Biochem Sci. 30, 250-255.

131. Kong, S.E.; Banks, C.A.; Shilatifard, A.; Conaway J.W.; Conaway R.C.; (2005) ELL-associated factors 1 and 2 are positive regulators of RNA polymerase II elongation factor ELL. Proc. Natl. Acad. Sci. USA 102, 10094-10098.

132. Jin, J.; Cai, Y.; Yao, T.; Gottschalk, A.; Florens, L.; Swanson, S.K.; Gutierrez, J.L.; Coleman, M.K.; Workman, J.L.; Mushegian, A.; Washburn, M.P.; Conaway, R.C.; Conaway, J.W. (2005) A Mammalian Chromatin Remodeling Complex with Similarities to the Yeast INO80 Complex. J. Biol. Chem. 280, 41207-41212.

133. Jin, J., Cai, Y., Li,B., Conaway, R.C., Workman, J.L., Conaway, J.W., and Kusch, T. (2005) In and out: histone variant exchange in chromatin. Trends Biochem. Sci. 30 680-687.

134. Ruhl, D.D., Jin, J. Cai, Y., Swanson S.K., Florens, L., Washburn, M.P., Conaway, R.C., Conaway, J.W., and Chrivia, J.C. (2006) Purification of a human SRCAP complex that remodels chromatin by incorporating the histone variant H2A.Z into nucleosomes. Biochemistry 45, 5671-5677.

135. Cai, Y., Jin, J., Gottschalk, A.J., Yao, T., Conaway, J.W., and Conaway R.C. (2006) Purification and assay of the human INO80 and SRCAP chromatin remodeling complexes. Methods 40, 312-317.

136. Yao, T., Song, L., Xu, W., DeMartino, G.N., Florens, L., Swanson, S.K., Washburn, M.P., Conaway, R.C., Conaway, J.W.*, and Cohen, R.E.* (2006) Proteasome recruitment and activation of the Uch37 deubiqutinating enzyme by Adrm1. Nature Cell Biology 8, 994-1002 (Epub 2006 Aug13).

137. Charlet-Berguerand, N., Feuerhahn, S., Kong, S.E., Ziserman, H., Conaway, J.W., Conaway, R.C., Egly, J.M. (2006) RNA polymerase II bypass of oxidative DNA damage is regulated by transcription elongation factors. EMBO J. 25, 5481-5491

138. Paoletti,A.C., Parmely,T.J., Tomomori-Sato,C., Sato,S., Zhu,D., Conaway,R.C., Conaway,J.W., Florens,L., Washburn,M.P. (2006) Quantitative proteomic analysis of distinct mammalian Mediator complexes using normalized spectral abundance factors. Proc. Natl. Acad. Sci. U.S.A. 103, 18928-18933.

84

139. Svejstrup, J.Q., Conaway, R.C., Conway, J.W. (2006) RNA polymerase II: A “Nobel” enzyme

demystified. Mol. Cell 24, 637-642.

140. Banks, C.A., Kong, S.E., Spahr, H., Florens, L., Martin-Brown, S., Washburn, M.P., Conaway, J.W., Mushegian, A., Conaway, R.C. (2007) Identification and characterization of a Schizosaccharomyces pombe RNA polymerase II elongation factor with similarity to the metazoan transcription factor ELL. J. Biol. Chem. 282, 5761-5769 (Epub ahead of print Dec 8, 2006).

141. Cai, Y., Jin, J., Yao, T., Gottschalk, A.J., Swanson, S.K., Wu, S., Shi, Y., Washburn, M.P., Florens, L., Conaway, R.C., Conaway, J.W. (2007) YY1 functions with INO80 to activate transcription, Nat. Struct. Mol. Biol. 14, 872-874.

142. Koutelou, E., Sato, S., Tomomori-Sato, C., Florens, L., Swanson, S.K., Washburn, M.P., Kokkinaki, M., Conaway, R.C., Conaway, J.W.*, Moschonas, N.* (2008) Neuralized-like 1 targeted to the plasma membrane by N-myristoylation regulates the Notch ligand Jagged1. J. Biol. Chem. 283, 3846-3853 (Epub ahead of print Dec 12, 2007).

143. Mahrour, N., Redwine, W.B., Florens, L., Swanson, S.K., Martin-Brown, S., Bradford, W.D., Staehling-Hampton, K., Washburn, M.P., Conaway, R.C., and Conaway, J.W. (2008) Characterization of cullin-box sequences that direct recruitment of Cul2-Rbx1 and Cul5-Rbx2 modules to elongin BC-based ligases. J. Biol. Chem. 283, 8005-8013 (Epub ahead of print Jan 10, 2008).

144. Sardiu, M.E., Cai, Y., Jin, J., Swanson, S.K., Conaway, R.C., Conaway, J.W., Florens, L., and Washburn, M.P. (2008) Modularity and interaction strength in a local human protein network. Proc. Natl. Acad. Sci., U.S.A. 105, 1454-1459 (Epub ahead of print Feb 5, 2008).

145. Gottschalk, A.J., Conaway, R.C., and Conaway, J.W. (2008) New clues to actin function in chromatin regulation. Nat. Struct. Mol. Biol. 15, 432-433. (News & Views)

146. Yao, T., Song, L., Jin, J., Cai, Y., Takahashi, H., Swanson, S.K., Washburn, M.P., Florens, L., Conaway, R.C., Cohen, R.E, and .Conaway, J.W. (2008) Distinct modes of regulation of the Uch37 deubiquitinating enzyme in the proteasome and in the INO80 chromatin remodeling complex. Mol. Cell 31, 909-917.

147. Conaway, R.C. and Conaway J.W. (2009) The INO80 chromatin remodeling complex in transcription, replication and repair. Trends Biochem. Sci. 34, 71-77 (Epub ahead of print Dec 4, 2008).

148. Yasukawa, T., Kamura, T., Kitajima, S., Conaway, R.C., Conaway, J.W., and Aso, T. (2008) Mammalian Elongin A complex mediates DNA-damage-induced ubiqutitylation and degradation of Rpb1. EMBO J. 27, 3256-3266.

85

149. Ding, N., Tomomori-Sato, C., Sato, S., Conaway, R.C., Conaway, J.W., and Boyer, T.G. (2009)

MED19 and MED26 are synergistic functional targets of the RE1 silencing transcription factor in epigenetic silencing of neuronal gene expression. J. Biol. Chem. 284 2648-2656.

150. Conaway, R.C. and Conaway, J.W. (2009) Mediator comes out from the shadows. Structure, 17, 485-486. (invited commentary)

151. Aygun, O., Xu, X., Liu, Y., Takahashi, H., Kong, S.E., Conaway, R.C., Conaway, J.W., and Svejstrup, J.Q. (2009) Direct inhibition of RNA polymerase II transcription by RECQL5. J. Biol. Chem. 284, 23197-23203.

152. Gottschalk, A.J., Timinszky, G., Kong, S.E., Jin, J., Cai, Y., Swanson, S.K., Washburn, M.P., Florens, L., Ladurner, A.G., Conaway, J.W., Conaway, R.C. (2009) Poly(ADP-ribosyl)ation directs recruitment and activation of an ATP-dependent chromatin remodeler. Proc. Natl. Acad. Sci. U.S.A. 106, 13770-13774.

153. Takahashi, H., Martin-Brown, S., Washburn, M.P., Florens, L., Conaway, J.W., Conaway, R.C. (2009) Proteomics reveals a physical and functional link between hepatocyte nuclear factor 4α and TFIID. J. Biol. Chem. 284, 32405-32412.

154. Liu, Y. and Conaway, J.W. (2009) When transcription meets recombination: a lesson from the human RECQ helicases. F1000 Biology Reports 1,76.

155. Harreman, M., Taschner, M., Sigurdsson, S., Anindya, R., Reid, J., Somesh, B., Kong, S.E., Banks, C.A.S., Conaway, R.C., Conaway, J.W., Svejstrup, J.Q. (2009) Distinct ubiquitin ligases act sequentially for RNA polymerase II polyubiquitylation. Proc. Natl. Acad. Sci. U.S.A. 106, 20705-20710.

156. Cai, Y., Jin, J., Swanson, S.K., Cole, M.D., Choi, S.H., Florens, L., Washburn, M.P., Conaway, J.W., Conaway, R.C. (2010) Subunit composition and substrate specificity of a MOF-containing histone acetyltransferase distinct from the Male-Specific Lethal (MSL) complex. J. Biol. Chem. 285, 4268-4272.

157. Lin, C., Smith, E.R., Takahashi, H., Lai, K.C., Martin-Brown, S., Florens, L., Washburn, M.P., Conaway J.W., Conaway, R.C., Shilatifard, A. (2010) Aff4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia. Mol Cell 37, 429-437.

158. Capotosti, F., Guernier, S., Lammers, F., Wairdel, P., Cai, Y., Jin, J., Conaway, J.W., Conaway, R.C., and Herr, W. (2011) O-GlcNAc transferase catalyzes site-specific proteolysis of HCF-1. Cell 144, 376-388.

86

159. Chen, L., Cai, Y., Jin, J., Florens, L., Swanson, S.K., Washburn, M.P., Conaway, J.W., Conaway,

R.C. (2011) Subunit organization of the human INO80 chromatin remodeling complex: An evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling. J. Biol. Chem. 285, 4268-4272.

160. Mushegian, A. and Conaway, J.W. (2011) Thematic minireview series: computational systems biology. J. Biol. Chem. 286, 23621-23622.

161. Conaway, R.C. and Conaway, J.W. (2011) Function and regulation of the Mediator complex. Curr. Opin. Genet. Dev. 21, 225-230.

162. Takahashi, H., Parmely, T.J., Sato, S., Tomomori-Sato, C., Banks, C.A., Kong, S.E., Szutorisz, H., Swanson, S.K., Martin-Brown, S., Washburn, M.P., Florens, L., Seidel, C., Lin, C., Smith, E.R., Shilatifard, A., Conaway, R.C., Conaway, J.W. (2011) Role for the human Mediator subunit Med26 as a docking site for transcription elongation factors. Cell 146, 92-104.

163. Conaway, R.C. and Conaway, J.W. (2011) Origins and activity of the Mediator Complex (2011) Seminars in Cell and Developmental Biology 22, 729-734.

164. Costessi, A., Mahrour, N., Tijchon, E., Stunnenberg, R., Stoel, M.A., Jansen, P.W., Sela, D., Martin-Brown, S., Washburn, M.P., Florens, L., Conaway, J.W., Conaway, R.C.*, Stunnenberg, H.G.* (2011) The tumor antigen PRAME is a subunit of a Cul2 ubiquitin ligase and associates with active NFY promoters. EMBO J., 30, 3786-3798.

165. Conaway, R.C. and Conaway, J.W. Von Hippel-Lindau (VHL) Protein. In Encyclopedia of Biological Chemistry, 2nd Edition, W.J. Lennarz and M.D. Lane, Eds., Elsevier, 2012.

166. Sela, D., Chen, L., Martin-Brown, S., Washburn, M.P., Florens, L., Conaway, J.W., Conaway, R.C. (2012) Endoplasmic reticulum stress-responsive transcription factor ATF6α directs recruitment of the Mediator of RNA polymerase II transcription and multiple histone acetyltransferases. J. Biol. Chem. 287, 2848-28494.

167. Yasukawa,T., Bhatt,S., Takeuchi,T., Kawauchi,J., Takahashi,H., Tsutsui,A., Muraoka,T., Inoue,M., Tsuda,M., Kitajima,S., Conaway,R.C., Conaway,J.W., Trainor,P.A., Aso,T. (2012) Transcriptional elongation factor Elongin A regulates retinoic acid-induced gene expression during neuronal differentiation. Cell Rep. 2, 1129-1136.

168. Conaway, R.C. and Conaway, J.W. (2012) The Mediator complex and transcription elongation. Biochim Biophys Acta. doi:pii: S1874-9399(12)00161-7. 10.1016/j.bbagrm.2012.08.017.

169. Costessi, A., Mahrour, N., Sharma, V., Stunnenberg, R., Stoel, M.A., Tijchon, E., Conaway, J.W., Conaway, R.C., and Stunnenberg, H.G. (2012) PloS One 7:e42822.

87

170. Gottschalk, A.J., Trivedi, R.D., Conaway, J.W., and Conaway, R.C. (2012) Activation of the SNF2 family ATPase ALC1 by poly(ADP-ribose) in a stable nucleosome•PARP1•ALC1 intermediate. J. Biol. Chem. 287, 43527-43532.

171. Tomomori-Sato, C., Sato, S., Conaway, R.C., and Conaway, J.W. (2013) Immunoaffinity purification of protein complexes from mammalian cells. Methods Mol Biol. 977, 273-287.

172. Tsai, K.L., Sato, S., Tomomori-Sato, C., Conaway, R.C., Conaway, J.W., Asturias, F.J. (2013) A conserved Mediator-CDK8 kinase module association regulates Mediator-RNA polymerase II interaction. Nat. Struct. Mol. Biol., 20, 611-619.

173. Kawauchi, J., Inoue, M., Fukuda, M., Uchida, Y., Yasukawa, T., Conaway, R.C., Conaway, J.W., Aso, T., Kitajima,S. (2013) Transcriptional properties of mammalian Elongin A and its role in stress response. J Biol. Chem. 288, 24302-24315.

174. Sela, D., Conkright, J.J., Chen, L., Gilmore, J., Washburn, M.P., Florens, L., Conaway, R.C., Conaway, J.W. (2013) Role for human Mediator subunit MED25 in recruitment of mediator to promoters by endoplasmic reticulum stress-responsive transcription factor ATF6α. J. Biol. Chem. 288, 26179-26187.

175. Chen, L., Conaway, R.C., Conaway, J.W. (2013) Multiple modes of regulation of the human Ino80 Snf2 ATPase by subunits of the INO80 chromatin remodeling complex. Proc. Natl. Acad. Sci., USA, in press.

Invited Presentations

Meetings

1990 Invited speaker, Gordon Conference on Nuclear Proteins, Gene Regulation, and Chromatin Structure, Tilton, New Hampshire.

1993 Invited speaker, Second Annual Austin Spring Meeting, "The Transcription Machine: Assembly and Function," Austin, Texas.

1993 Invited speaker, Gordon Conference on Nucleic Acids, New Hampton, New Hampshire.

1994 Invited speaker, Keystone Symposium on "Basic Aspects of Transcription," Keystone, Colorado.

1995 Invited speaker, Gordon Conference on Nucleic Acids, New Hampton, New Hampshire.

1995 Special Lecturer, 10th Asagiri Symposium, Asagiri, Japan.

1996 Invited speaker, Keystone Symposium on Transcription, Taos, New Mexico.

88

1996 Invited speaker, 1996 Gordon Conference on Molecular Genetics, Newport, Rhode

Island

1996 Invited speaker, FASEB Meeting on Transcription, Snowmass, Colorado

1997 Invited speaker, Cold Spring Harbor Meeting on "Mechanisms of Eukaryotic Transcription," Cold Spring Harbor Laboratories, Cold Spring Harbor, New York.

1997 Invited speaker, Symposium on Basic Mechanisms of Transcription Initiation, Elongation, and Termination, 17th International Congress of Biochemistry and Molecular Biology and 1997 Annual Meeting of the American Society for Biochemistry and Molecular Biology, San Francisco, California.

1997 Invited speaker, American Association for Cancer Research Special Conference on Transcriptional Control of Proliferation, Differentiation, and Development, Bolton's Landing, New York

1997 Invited speaker, American Society for Nephrology Symposium on RNA Processing, San Antonio, Texas

1998 Invited speaker, Keystone Symposium on Transcriptional Mechanisms, Taos, New Mexico.

1998 Invited speaker, Ben May Cancer Biology Symposium, "Regulation of Gene Expression and Profileration", Chicago, Illinois

1998 Invited speaker, 63rd Cold Spring Harbor Symposium on Quantitative Biology, "Mechanisms of Transcription", Cold Spring Harbor, New York

1998 Invited speaker, FASEB Summer Research Conference on "Transcriptional Regulation during Cell Growth, Differentiation and Development", Snowmass Colorado.

1998 Invited speaker, EMBL Transcription Meeting, Heidelberg, Germany.

1999 Invited speaker, Keystone Symposium on the Molecular Basis of Cancer

1999 Invited speaker, Sixth Cold Spring Harbor Meeting on Mechanisms of Eukaryotic Transcription, Cold Spring Harbor, New York

1999 Invited speaker, Jaques Monod Conference on Transcription and Development, Roscoff, France.

2000 Invited Speaker, FASEB Summer Research Conference on "Transcriptional Regulation during Cell Growth, Differentiation and Development", Snowmass Colorado.

2000 Invited Speaker, Molecular Genetics Gordon Conference, Connecticut College, New London, CT.

2000 Invited speaker, Keystone Symposium on the Molecular Basis of Cancer 2001 Invited speaker, Keystone Symposium on Transcription Mechanisms, Santa Fe, NM 2002 Invited speaker, Seventh Cold Spring Harbor Meeting on Mechanisms of Eukaryotic

Transcription, Cold Spring Harbor, NY

2002 Invited speaker, FASEB Summer Research Conference on “Transcriptional Regulation during Cell Growth, Differentiation, and Development”, Saxton’s River, VT

2003 Invited speaker, Keystone Symposium on the Enzymology of Chromatin and Transcription, Santa Fe, NM

89

2003 Invited speaker, NIDDK workshop on “Ubiquitin and Ubiquitin-like Modifications in

Health and Disease. National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD.

2004 Invited speaker, Workshop on “Ubiquitin in Cancer and in Chronic Diseases.” The Hebrew University of Jerusalem and the Institute for Advanced Studies, Giv’at Ram, Jerusalem.

2004 Lecturer on “Regulation of Transcription Initiation and Elongation,” 2004-2007 Cold Spring Harbor Gene Expression Course, Cold Spring Harbor Laboratory.

2004 Invited speaker, FASEB Summer Research Conference on “Transcriptional Regulation During Cell Growth, Differentiation, and Development,” Saxton’s River, VT.

2004 Invited speaker, 6th EMBL Transcription Meeting, Heidelberg, Germany.

2004 Invited speaker, 130th Nobel Symposium (Chemistry) on “Molecular Mechanisms in Biological Systems,” Tällberg, Dalarna, Sweden.

2004 Keynote speaker, ASBMB Fall Symposium on “Transcriptional Regulation by Chromatin and RNA Polymerase II,” Granlibakken, Lake Tahoe, CA.

2005 Invited speaker and Discussion Leader, Nucleic Acids Gordon Conference, Newport, R. I.

2005 Plenary speaker, 7th International Symposium on Mass Spectrometry in the Health and Life Sciences

2006 Invited speaker, Keystone Symposium on “Nucleic Acid Enzymes,” Taos, NM

2006 Invited speaker, Keystone Symposium on “Regulation of Eukaryotic Transcription: From Chromatin to mRNA,” Taos, NM

2006 Plenary speaker, Midwest Meeting on Chromatin, Transcription, and Nuclear Dynamics, Iowas City, IA

2006 Invited speaker, FASEB Summer Research Conference on “Transcriptional Regulation During Cell Growth, Differentiation, and Development,” Saxton’s River, VT.

2006 Invited speaker, ASBMB Special Symposium, “Transcriptional Regulation by Chromatin and RNA Polymerase II,” Kiawah Island, SC.

2007 Invited speaker, Salk Institute, Fondation IPSEN, and Nature Symposium on Biological Complexity, “Diseases of Transcription,” La Jolla, CA

2007 Speaker, Cold Spring Harbor Meeting on Mechanisms of Eukaryotic Transcription, Cold Spring Harbor, NY

2007 Invited speaker, Joint Annual Meetings of the Molecular Biology Society of Japan and Japanese Biochemical Society, Yokohama, Japan

2008 Invited speaker, Keystone Symposium on “Regulatory Mechanisms in Eukaryotic Transcription,” Keystone, CO.

2008 Invited speaker, FASEB Summer Research Conference on “Transcriptional Regulation During Cell Growth, Differentiation, and Development,” Snowmass, CO.

2008 Invited speaker, Benzon Symposium No. 55, “Transcription, chromatin, and disease,” Copenhagen, Denmark

2008 Invited speaker, 8th EMBL Transcription Meeting, Heidelberg, Germany

90

2009 Invited speaker, “Proteomic characterization of macromolecular complexes involved

in DNA metabolism,” Trieste, Italy

2009 Invited speaker, Keystone Symposium on “Deregulation of transcription in cancer: Controlling cell fate decisions,” Killarney, Co. Kerry, Ireland.

2009 Invited speaker, Cold Spring Harbor Meeting on Mechanisms of Eukaryotic Transcription, Cold Spring Harbor, NY

2010 Invited speaker, Keystone Symposium "Dynamics of Eukaryotic Transcription During Development," Big Sky, Montana

2012 Invited speaker, ASBMB Annual Meeting, San Diego, CA.

2012 Invited speaker, ASBMB Special Symposium "Transcriptional Regulation: Chromatin and RNA Polymerase II," Snowbird, Utah.

2013 Invited speaker, Japanese Biochemical Society Hokkaido Division, Sapporo, Japan.

2013 Keynote speaker, Transcription Cycle Symposium, Hakone, Japan.

Seminars

1989 DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California.

1989 Department of Biology, Haverford College, Haverford, Pennsylvania.

1991 Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma.

1992 Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio.

1992 Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale, Illinois.

1992 Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma.

1992 University of Colorado Health Science Center, Denver, Colorado.

1993 Department of Biochemistry, University of Texas Southwestern Medical School, Dallas, Texas.

1993 Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia.

1993 Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, Canada.

1994 Department of Biochemistry and Molecular Biology, Texas Tech Health Sciences Center, Lubbock, Texas

1994 Fred Hutchinson Center for Cancer Research, Seattle, Washington.

1994 DNAX Research Institute for Molecular and Cell Biology, Palo Alto, CA.

1994 Tumor Biology Seminar Series, Stanford University School of Medicine, Stanford, CA.

1995 Department of Molecular and Cell Biology, University of California, Berkeley, CA.

91

1995 Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston,

TX.

1995 Department of Medicine, Louisiana State University Medical Center, Shreveport, LA.

1995 National Institute of Genetics, Mishima, Japan.

1995 Institute of Medical Sciences, University of Tokyo, Japan.

1995 Dana-Farber Cancer Institute, Boston, MA.

1995 Division of Immunology, Department of Medicine, Cornell Medical Center, New York, NY.

1996 Cancer Therapy and Research Center, San Antonio, Texas.

1996 Department of Chemistry and Biochemistry, University of Texas, Austin, Texas. 1996 Department of Biochemistry, School of Public Health, Johns Hopkins University, Baltimore, MD.

1996 Laboratory of Molecular Embryology, NICHD, NIH, Bethesda, MD.

1996 Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH.

1996 Department of Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA.

1996 Eukaryotic Gene Expression Course, Cold Spring Harbor Laboratories, Cold Spring Harbor, NY.

1996 Tumor Biology Seminar Series, Stanford University School of Medicine

1997 ACCESS Seminar Series, University of California, Los Angeles

1997 Department of Molecular Genetics, M. D. Anderson Center for Cancer Research, Houston, TX

1997 Huntsman Cancer Institute, University of Utah, Salt Lake City, UT

1997 Department of Pathology, University of Oklahoma Health Science Center, Oklahoma City, OK

1997 Cleveland Clinic, Cleveland OH.

1997 Banting and Best Institute, University of Toronto, Ontario, Canada

1997 ASBMB-Amgen Award Lecture, San Francisco, CA.

1997 Department of Medicine, Medical College of Georgia, Augusta, GA.

1997 Department of Microbiology, University of Texas-Southwestern Medical Center, Dallas, TX.

1997 Department of Biochemistry, Texas Tech University, Lubbock, TX.

1997 Department of Biochemistry, University of Kansas Medical Center, Kansas City, Kansas.

1998 Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY.

1998 Department of Biochemistry and Molecular Biology, University of Oklahoma Health Science Center, Oklahoma City, OK.

1998 Dana-Farber Cancer Institute, Boston, MA. 1998 Department of Biochemistry, Duke University School of Medicine, Durham, NC.

92

1999 Noble Foundation, Ardmore, OK.

1999 Department of Biochemistry, Louisiana State University Medical Center, New Orleans, LA.

1999 Department of Biochemistry, Michigan State University, East Lansing, MI.

1999 Department of Biochemistry, Tufts University School of Medicine, Boston, MA

1999 Department of Biological Sciences, Stanford University, Stanford, CA

1999 Department of Biochemistry, Saint Louis University School of Medicine, Saint Louis, MO

1999 Department of Tumor Cell Biology, St. Jude Children’s Research Hospital, Memphis, TN

2000 Department of Biochemistry, Oklahoma State University, Stillwater, OK

2000 Dean’s Distinguished Lecture and Department of Molecular and Cellular Physiology Seminar Series, University of Cincinnati School of Medicine, Cincinnati, OH

2000 The Salk Institute for Biological Studies, San Diego, CA

2000 Medical Scientist Lecture Series and Department of Biological Chemistry, University of California, Irvine, CA.

2000 National Cancer Institute, Bethesda, MD

2000 Stowers Institute for Medical Research, Kansas City, MO

2001 Huntsman Cancer Institute, University of Utah, Salt Lake City, UT

2001 Department of Biochemistry, Emory University School of Medicine, Atlanta, GA

2001 Department of Biochemistry, New Jersey Medical School, Newark, NJ

2001 Dean’s Distinguished Lecturer, Medical College of Ohio, Toledo, OH 2001 Burroughs-Wellcome Visting Professorship Lecture, Department of Biochemistry, Saint Louis

University School of Medicine, St. Louis, MO

2001 Distinguished Lecturer, University of Buffalo, Buffalo, NY

2001 Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS

2002 Ontario Cancer Institute, Toronto, Ontario, Canada 2002 Institut de Recherches Cliniques de Montreal, Montreal, Quebec, Canada

2002 Tumor Biology Seminar Series, Stanford University School of Medicine

2002 Department of Biological Sciences, University of Missouri, Columbia, MO

2003 Milton S. Hershey Medical Center College of Medicine, Hershey, PA

2003 Cold Spring Harbor Laboratories, Cold Spring Harbor, NY

2003 University of California, San Francisco, CA

2003 Department of Molecular Biology, Princeton University, Princeton, NJ

2003 Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH

2003 Graduate Program in Molecular, Cellular, and Developmental Biology, Ohio State University, Columbus, OH

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2004 Department of Biochemistry and Molecular Genetics, University of Illinois at

Chicago, Chicago, IL

2004 Department of Molecular Biosciences, University of Kansas, KS

2004 Cancer Center, St. Louis University, St. Louis, MO.

2005 Eppley Cancer Institute, Universty of Nebraska Medical Center, Omaha, NE

2005 Biochemistry and Molecular Biology Seminar Series, Mayo Clinic College of Medicine, Rochester, MN

2005 Pennington Biomedical Research Center, Baton Rouge, LA

2005 University of North Carolina Lineberger Comprehensive Cancer Center, Chapel Hill, NC

2005 Department of Biochemistry and Molecular Biology, University of Missouri, Columbia, MO

2005 Department of Pharmacology, University of Michigan, Ann Arbor, MI

2006 Department of Biochemistry, Dartmouth Medical School, Hanover, NH

2006 Laboratory of Gene Regulation and Development, NICHD, NIH, Bethesda, MD

2006 Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL

2006 Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO

2006 Department of Biochemistry, Vanderbilt University, Nashville, TN

2006 Frontiers in Biology Seminar Series, Biochemistry Department, Stanford University, Stanford, CA

2006 Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Iraklion, Crete.

2007 Department of Biochemistry and Molecular Biology, University of Kentucky, Lexington, KY

2007 Osaka University, Osaka, Japan

2007 Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan

2008 Department of Biochemistry and Molecular Genetics, University of Virginia

2008 Department of Chemistry and Biotechnology/Life Sciences Seminar, Beadle Center, University of Nebraska.

2008 Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK.

2009 Cancer Research UK, Clare Hall Laboratories, South Mimms, United Kingdom

2009 NIH Transcription Factor Interest Group, National Insitutes of Health, Bethesda, MD.

2010 Department of Structural Biology, University of Pittsburgh, Pittsburgh, PA

2010 Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO.

2011 "Science at the Edge Seminar Series," Michigan State University, East Lansing, MI

2011 School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO

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2011 Department of Pharmacology, New York University, New York, NY

2011 Cancer Center, Massachusetts General Hospital, Charlestown, MA

2011 Department of Biochemistry & Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD

2012 Department of Biochemistry & Molecular Biology, Oklahoma State University, Stillwater, OK.

2013 Department of Biochemistry, Duke University School of Medicine, Durham, NC

2013 Tokyo Medical and Dental University, Tokyo, Japan

2013 Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY

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Aron W. Fenton

Education B.S. Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, 1993

Ph.D. Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, 1999 (Dr. James B. Blair, advisor)

Postdoctorate, Biochemistry and Biophysics, Texas A&M University, College Station, TX (Dr. Gregory D. Reinhart, advisor)

Major Areas of Interest

Allosteric Regulation of Enzymes

Enzymatic Control of Metabolic Pathways

Protein Structure/Function in Signal Transduction Pathways

Metabolic Engineering of Cyanobacteria to Optimize Alkane (Diesel) Production

Research Experience

2010-present: Associate Professor, Department of Biochemistry and Molecular Biology, The University of Kansas Medical Center, Kansas City, KS. The Mechanism of allosteric regulation in pyruvate kinase. 2004-2010: Assistant Professor, Department of Biochemistry and Molecular Biology, The University of Kansas Medical Center, Kansas City, KS. Thermodynamics of allosteric regulation of pyruvate kinase. 2006: Visiting Scholar, School of Molecular and Microbial Biosciences, The University of Sydney, Sydney, Australia. Small angle x-ray scattering of pyruvate kinase (Dr. Jill Trewhella, host). 1999-2003: Postdoctoral Research Associate, Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX. Thermodynamics of allosteric regulation of E. coli phosphofructokinase (Dr. Gregory D. Reinhart, advisor).

Updated February 15, 2013 Department of Biochemistry and Molecular Biology The University of Kansas Medical Center MS 3030, 3901 Rainbow Boulevard Kansas City, Kansas 66160 (913)-588-7033 e-mail: afenton@kumc.edu

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1994–1999: Graduate Research Associate, Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK. Allosteric regulation of yeast pyruvate kinase (Dr. James B. Blair, advisor). 1994: Research Technician, Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK. Hormonal regulation of mammalian carbohydrate metabolism (Dr. James B. Blair, principle investigator). 1993: Undergraduate Research Technician, Department of Animal Science, Oklahoma State University, Stillwater, OK. Satiety factors in swine (Dr. Archie Clutter, principle investigator). 1992-1993: Special Research Student, Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK. Components of venom (Dr. George Odell, advisor).

Professional Memberships and Honors

Selected to co-organize the 2013 Gibbs Conference in Biothermodynamics

ACS-Kansas City chapter guest speaker to class of 2012 student honorees

Outstanding Instructor 2010-2011, The University of Kansas Medical Center, by graduate student vote

American Chemical Society

Biophysical Society

Protein Society

Oklahoma State University Alumni, Lifetime member

Phi Lambda Upsilon, National Honorary Chemical Society, Lifetime member

Gamma Sigma Delta, Honor Society of Agriculture, Lifetime member

American Institute of Chemists Award

College of Agriculture Fleming Scholarship

Teaching Experience

2012: Instructor in Interdisciplinary Graduate Program in Biomedical Sciences covering topics of ligand binding and enzyme kinetics, The University of Kansas Medical Center 2009-present: Medical School lecturer covering topics of Energy Metabolism, Medical Biochemistry, The University of Kansas Medical Center 2009-present: Instructor in Interdisciplinary Graduate Program in Biomedical Sciences covering topics of Energy Metabolism, The University of Kansas Medical Center 2008: Direct graduate course covering the thermodynamic aspects of

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allosteric and cooperative regulations. This course was taught as an independent study. 2006-2009: Develop and maintain an e-learning activity on enzymology for the use of medical student education, The University of Kansas Medical Center 2006-present: Invited topic lecture, “Allosteric regulation.” The University of Kansas-Edwards campus, Kansas City, KS 2005-present: Discussion Group Leader, Medical Biochemistry 801/802, The University of Kansas Medical Center 2004-2008: Instructor and Course Director, Module II Bchm892: Selected Topics in Cell Metabolism, Interdisciplinary Graduate Program in Biomedical Sciences, The University of Kansas Medical Center 1994-1999: Teaching Assistant, Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK

Visiting Scientists Host

David Pedergrass, 2005-2007 KUMC The University of Kansas-Edwards Campus

Postdoctoral Fellow Advisor

Charulata Prasannan 2010-2012 Ph.D. KUMC

Aileen Alontaga 2008-2010 Ph.D. KUMC

Graduate Student Advisor

Lu Chen 2008-Present Ph.D. KUMC/Stowers

Co-Advisor

Rushi Trivedi 2011-Present Ph.D. KUMC/Stowers

Co-Advisor

Graduate Student Committee Member

Mary Ashley Rimmer 2012-Present Ph.D. KUMC

John “J.P.” McGinnis 2012-Present Ph.D. KUMC/Stowers

Danny Miller 2012-Present Ph.D. KUMC/Stowers

Ram Kannan 2012-Present Ph.D. KUMC/Stowers

Evan Janzen 2011-Present Ph.D. KUMC/Stowers

Dan Parente 2010-Present Ph.D. KUMC

Mauricio Vargas-Uribe 2010-Present Ph.D. KUMC

Ben Deng 2009-2011 Ph.D. KUMC

Subhashchandra Naik 2008-2013 Ph.D. KUMC

Troy Johnson 2008-2012 Ph.D. KUMC

Ozan Kumru 2008 Ph.D. KUMC Qualifying committee only

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Hiroo Katayama 2006-2012 Ph.D. KUMC

Viktor Matskevich 2006 Ph.D. KUMC Qualifying committee only

Alex Dajković 2005 Ph.D. KUMC

Greg Bomhoff 2004-2005 M.S. KUMC

Graduate Student Research Rotation Advisor

Arjun Ishwar 2012 M.S. KUMC

Nancy Stiles 2011 Ph.D. KUMC

Cassandra Field 2011 Ph.D. KUMC

Jason Barnett 2010 Ph.D. KUMC

Mitchell McGill 2008 Ph.D. KUMC

Rachel Williams 2005 Ph.D. KUMC

Coleen Flynn 2005 Ph.D. KUMC

Andrew Bigley 2003 Ph.D. Texas A&M

Anand Venkatraman 2001 Ph.D. Texas A&M

Carrie Lagace 2000 Ph.D. Texas A&M

Peter Cornish 2000 Ph.D. Texas A&M

Jun-Sung Lim 1999 M.S. OSU

Gene Harris 1998 Ph.D. OSU

Bobby Gramling 1998 Ph.D. OSU

SoYeon Park 1995 M.S. OSU

Mellisa Sims 1994 Ph.D. OSU

Undergraduate Student Researcher Advisor

Nick Domann 2012- KU-Edwards Research Student, KUMC present

Wendy Alvarez 2012- Rockhurst Research Student, KUMC

present

Lin Lau 2012 Creighton University Student, KUMC

Michael Gagliano 2012 Kansas State University Student, KUMC

James Urness 2009- Rockhurst Research Student, KUMC 2012

Jena Skillet 2009 KU-Edwards Research Student, KUMC

Danny Miller 2008- Rockhurst Research Student, KUMC 2011

Kristen Morphew 2008 Johnson County Community College, KUMC

Julie Nguyen 2008 KU-Edwards Research Student, KUMC

Gilbert Ako 2008 KU-Edwards Research Student, KUMC

Tyler Marquart 2007 Rockhurst Research Student, KUMC

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Khedidja Beldjilali 2006- KU-Edwards Research Student, KUMC 2007

Kelly Thuet 2006- Rockhurst Research Student, KUMC 2007

Cynthia Costa 2006 KU-Edwards Research Student, KUMC

Natasha Kaur 2006 KU-Edwards Research Student, KUMC

John VanNice 2005 KU-Edwards Research Student, KUMC

Mathews Athiyal 2005 KU-Edwards Research Student, KUMC

Melinda Stump 2004 Visiting High School Teacher, KUMC

Gissel McDonald 2004 Visiting High School Teacher, KUMC

Ann Menefee 2002 Research Student, Texas A&M

Keisha Hardeman 2000 Anakletic Laboratory Program in Sciences, Texas A&M

Justin Turner 2000 Office of Naval Research Program, Texas A&M

Angela Krueger 1999 Oklahoma Partners in Biological Science, OSU

George Wiland 1998 Research Student, OSU

Catie Howard 1998 Research Student, OSU

Jeff Frazier 1997 Oklahoma Partners in Biological Science, OSU

Colby Fuser 1997 Oklahoma Partners in Biological Science, OSU

Caleb Lack 1997 Freshman Scholar, OSU

Amber Coulter 1997 Freshman Scholar, OSU

Lori Davis 1996 Wentz Scholar, OSU

Philip Morton 1996 Wentz Scholar, OSU

Windy Ives 1995 Wentz Scholar, OSU

Punsasi Rajakaiuna 1995 Research Student, OSU

John Carment 1994 Freshman Scholar, OSU

High School Student Researcher Advisor

Sang Lau 2012 ACS Seed High School Student, KUMC

Christine Shultz 2012 Olathe Biotech High School Student SMART team protein modeling, KUMC

Nehal Patel 2012 Olathe Biotech High School Student SMART team protein modeling, KUMC

Jennifer Wuelzer 2012 Olathe Biotech High School Student SMART team protein modeling, KUMC

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Bridgette Begot 2012 Olathe Biotech High School Student SMART team protein modeling, KUMC

Karla Torres 2010 ACS Seed High School Student, KUMC

Anndrea Fenton 2010 Olathe Biotech High School Student, KUMC

Michael Chopade 2009 Olathe Biotech High School Student, KUMC

Josh Smith 2009 Spring Hill High School Honors Student, KUMC

Holly Hrabik 2008 Olathe Biotech High School Student SMART team protein modeling, KUMC

Kristen Morphew 2008 Olathe Biotech High School Student SMART team protein modeling, KUMC

Irene Wang 2006- Olathe Biotech High School Student, 2007 KUMC

Jing Huang 2006 Olathe Biotech High School Student, KUMC

Doanh Tran 2004- ACS Seed High School Student, 2005 KUMC

Publications

1. Ishwar, A., Tang, Q., and Fenton, A.W. (2013) Distinguishing the interactions in the fructose-1,6-bisphosphate binding site of human liver pyruvate kinase that contribute to allostery. Submitted.

2. Prasannan, C.B., Villar, M.T., Artigues, A., and Fenton, A.W. (2013) Identification of regions of rabbit muscle pyruvate kinase important for allosteric regulation by phenylalanine, detected by H/D exchange mass spectrometry. Submitted.

3. Holyoak, T., Zhang, B., Deng, J., Tang, Q., Prasannan, C.B., Fenton, A.W. (2013) Energetic coupling between an oxidizable cysteine and the phosphorylatable N-terminus of human liver pyruvate kinase. Biochemistry 52, 466-467. –Featured article on Biochemistry web site.

4. Urness, J.M., Clapp, K.M., Timmons, J.C., Bai, X., Chandrasoma, N., Buszek, K.R., and Fenton, A.W. (2013) Distinguishing the chemical moiety of phosphoenolpyruvate that contributes to allostery in muscle pyruvate kinase. Biochemistry 52, 1-3.

5. Fenton, A.W. (2012) Identification of allosteric activating drug leads for human liver pyruvate kinase. Book chapter in Allostery: Methods and Protocol 796, 369-382 in the Methods in Molecular Biology series.

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6. Prasannan, C.B., Tang, Q., and Fenton, A.W. (2012) Allosteric regulation of human liver pyruvate kinase by peptides that mimic the phosphorylated/dephosphorylated N-terminus. Book chapter in Allostery: Methods and Protocol 796, 335-349 in the Methods in Molecular Biology series.

7. Miller, D.E., Prasannan, C.B., Villar, M.T., Fenton, A.W., and Artigues, A. (2012) HDXAnalyzer: Automated analysis and data reporting of deuterium/hydrogen exchange mass spectrometry. J Am Soc Mass Spectrom. 23, 425-429.

8. Prasannan, C.B. Artigues, A. and Fenton, A.W. (2011) Monitoring allostery in D2O: a necessary control in studies using hydrogen/deuterium-exchange to characterize allosteric regulation Anal Bioanal Chem, 401, 1083-1086..

9. Alontaga, A.Y., and Fenton, A.W. (2011) Effector Analogues Detect Varied Allosteric Roles for Conserved protein-effector interactions in pyruvate kinase isozymes. Biochemistry, 50, 1934-1939.

10. Villar, M.T., Miller, D.E., Fenton, A.W. and Artigues, A. (2010) SAIDE: A Semi-Automated Interface for Hydrogen/Deuterium Exchange Mass Spectrometry. Proteomica, 6, 63-69.

11. Fenton, A.W., Johnson, T.A., and Holyoak, T. (2010) The pyruvate kinase model system, a cautionary tale for the use of osmolyte perturbations to support conformational equilibria in allostery. Protein Sci. 19, 1796-800.

12. Fenton, A.W., Williams, R., and Trewhella, J. (2010) Changes in small-angle X-ray scattering parameters observed upon binding of ligand to rabbit muscle pyruvate kinase are not correlated with allosteric transitions. Biochemistry, 49, 7202-7209.

13. Fenton, A.W. and Alontaga, A.Y. (2009) The Impact of Ions on Allosteric Functions in Human Liver Pyruvate Kinase. Methods in Enzymology, 466, 83-106.

14. Fenton, A. W., and Reinhart, G. D. (2009) Disentangling the Web of Allosteric Communication: Quantification of the Four Unique Heterotropic Inhibiting Interactions in E. coli Phosphofructokinase. Biochemistry, 48, 12323-12328.

15. Fenton, A.W. and Tang, Q. (2009) An activating interaction between the unphosphorylated N-terminus of human liver pyruvate kinase and the main body of the protein is interrupted by phosphorylation.

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Biochemistry, 48, 3816-3818.

16. Fenton, A.W. and Hutchinson, M. (2009) The pH dependence of the allosteric response of human liver pyruvate kinase to fructose-1,6-bisphosphate, ATP, and alanine. Archives of Biochem. And Biophys. 484, 16-23. 17. Fenton, A. W. (2008) Allostery: an illustrated definition for the ‘second secret of life’, Trends in Biochem. Sci. 33, 420-425. 18. Williams, R., Holyoak, T., McDonald, G., Gui, C., and Fenton, A. W. (2006) Differentiating a Ligand’s Chemical Requirements for Allosteric Interactions from Those for Protein Binding; Phenylalanine Inhibition of Pyruvate Kinase. Biochemistry 45, 5421-5429 19. Pendergrass, D. C., Williams, R., Blair, J. B., and Fenton, A. W. (2006) Mining for Allosteric Information: Natural Mutations and Positional Sequence Conservation in Pyruvate Kinase. IUMBM Life 58, 31-38. 20. Fenton, A. W., Paricharttanakul, N. M., and Reinhart, G. D. (2004) Disentangling the Web of Allosteric Communication in a Homotetramer: Heterotropic Activation in Phosphofructokinase from Escherichia coli. Biochemistry 43, 14104-14110. 21. Fenton, A. W. and Reinhart, G. D. (2003) Mechanism of Substrate Inhibition in Escherichia coli Phosphofructokinase. Biochemistry 42, 12676-12681. 22. Fenton, A. W., Paricharttanakul, N. M., and Reinhart, G. D. (2003) Identification of Substrate Contact Residues Important for the Allosteric Regulation of Phosphofructokinase from E. coli. Biochemistry 42, 6453-6459. 23. Fenton, A. W., Paricharttanakul, N. M., and Reinhart, G. D. (2003) Allosteric Inhibition and Activation in E. coli Phosphofructokinase use the Same Binding Sites but Different Pathways. Biophysical Journal 84, 169a. 24. Fenton, A. W., and Reinhart, G. D. (2002) Isolation of a Single Activating Interaction in E. coli Phosphofructokinase. Biochemistry 41, 13410-13416. 25. Fenton, A. W., and Reinhart, G. D. (2002) Binding Energy is Not Conferred by Binding Site Residues Alone; the Binding Site of E. Coli Phosphofructokinase. Biophysical Journal 82, 329a. 26. Fenton, A. W., and Blair, J. B. (2002) Kinetic and Allosteric Consequences of Mutations in the Subunit and Domain Interfaces and the Allosteric Site of Yeast Pyruvate Kinase. Archives of Biochemistry and Biophysics 397, 28-39. -Cover Art.

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27. Fenton, A. W., and Reinhart, G. D. (2001) Quantification of Isolated Heterotropic Interactions Contributing to Allostery in Phosphofructokinase from E. coli. Biophysical Journal 80, 309a. 28. Fenton, A. W., and Reinhart, G. D. (2000) Formation of a Modified Tetramer of E. coli Phosphofructokinase with Only One Native Active Site and One Native Allosteric Site. Protein Science 9, 129. 29. Lambdin, J. M., Howell, D. E., Kocan, K. M., Murphey, D. R., Arnold, D. C., Fenton, A. W., Odell, G. V., and Ownby, C. L. (2000) The Venomous Hair Structure, Venom and Life Cycle of Lagoa Crispata, A Puss Caterpillar of Oklahoma. Toxicon 38, 1163-1189. 30. Odell, G. V., Fenton, A. W., Ownby, C. L., Doss, m. P., and Schmidt, J. O. (1999) The Role of Venom Citrate. Toxicon 37, 407-409. 31. Odell, G. V., Ferry, P. C., Vick, L. M., Fenton, A. W., Decker, L. S., Cowell, R. L., Ownby, C. L., and Gutierrez, J. M. (1998) Citrate Inhibition of Snake Venom Proteases. Toxicon 36, 1801-1806. 32. Fenton, A. W., West, P. R., Odell, G. V., Hudiburg, S. M., Ownby, C. L., Mills, J. N., Scroggins, B. T., and Shannon, S. D. (1995) Arthropod Venom Citrate Inhibits Phospholipase A2. Toxicon 33, 763-770.

Books Edited

1. Fenton, A.W. ed. (2012) Allostery: Methods and Protocol in the Methods in Molecular Biology series.

Poster Presentations

1. Aron W. Fenton, Todd Holyoak, Bing Zhang, Junpeng Deng, Charulata B. Prasannan and Qingling Tang (2013) Inhibition of human liver pyruvate kinase by phosphorylation and cysteine oxidation share a regulatory mechanism, removal of an activating interaction between the N-terminus and the main body of the protein. 23rd Enzyme Mechanisms Conference, Coronado, CA.

2. Aron W. Fenton, Todd Holyoak, Bing Zhang, Junpeng Deng, Charulata B. Prasannan and Qingling Tang (2012) Inhibition of human liver pyruvate kinase by phosphorylation and cysteine oxidation share a regulatory mechanism, removal of an activating interaction between the N-terminus and the main body of the protein. The Sixth Peptide Engineering Meeting (PEM6), Atlanta, GA.

3. Aron W. Fenton, Todd Holyoak, Charulata B. Prasannan and Qingling Tang (2012) Inhibition of human liver pyruvate kinase by phosphorylation at Ser12 and oxidation at Cys436 share a regulatory mechanism, removal of an activating interaction between the N-terminus and the main body of the protein. Southeastern Enzyme Conference, Atlanta, GA.

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4. Prasannan, C.B. Villar, M.T., Artigues, A. and Fenton, A.W. (2012) Hydrogen/deuterium exchange mass spectrometry identification of allosterically relevant changes in rabbit muscle pyruvate kinase Gordon Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH.

5. Fenton, A.W. and Tang, Q. (2011) Mapping allostery in liver pyruvate kinase using a whole protein, alanine-scan. Gordon Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH.

6. Fenton, A.W. Urness, J., Alontaga, A.Y., Thuet, K., Barnett, J. and Timmons, C. (2011) Identification of the allosteric response element(s) in the active site of mammalian pyruvate kinase isozymes. 22nd Enzyme Mechanisms Conference, St. Pete Beach, FL.

7. Fenton, A.W. and Tang, Q. (2009) Mapping Individual residues within the N-terminus that are energetically coupled to allosteric and active site functions of human liver pyruvate kinase. Keystone Symposia, Keystone, CO. 8. Fenton, A. W., Thuet, K., Williams, R. E., and Trewhella, J. (2007) Identifying Allosteric Specific Structural Changes in Rabbit Muscle Pyruvate Kinase. Midwest Regional ACS Meeting, Kansas City, MO. 9. Fenton, A. W., Thuet, K., Williams, R. E., and Trewhella, J. (2007) Identifying Allosteric Specific Structural Changes in Pyruvate Kinase. 21st Annual Gibolbs Conference on Biothermodynamics, Carbondale, Il. 10. Fenton, A. W. and Hutchinson, M. (2007) The Ser12 to Asp Mutation of Human Liver Pyruvate Kinase Mimics Regulatory Effects of Phosphorylation at Ser12. The 21st Symposium of the Protein Society, Boston MA. 11. Thuet, K., Williams, R. E., Trewhella, J., and Fenton, A. W. (2007) Non-allosteric Effector Analogs, Unique Tools for Identifying Allosteric Specific Structural Changes in Pyruvate Kinase. 51st Annual Biophysical Society Meeting, Baltimore, MD. 12. Hutchinson, M., Fan, L. and Fenton, A. W. (2006) Purification of Human Liver Pyruvate Kinase from a Newly Constructed E. coli Strain Deficient in Endogenous Pyruvate Kinase Activity. Midwest Enzyme Conference, Chicago, Il. 13. Thuet, K., Williams, R. E., Trewhella, J., and Fenton, A. W. (2006) Non-allosteric Analogs, Unique Tools for Identifying Allosteric Specific Structural Changes in Pyruvate Kinase. Midwest Enzyme Conference, Chicago, Il. 14. Dajkovic, Alex, Fenton, A., and Lutkenhaus, J. (2006) A model for cooperative polymerization in a linear polymer. 20th Annual Gibbs

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Conference on Biothermodynamics, Carbondale, Il. 15. Fenton, A. W., Williams, R., and Holyoak, T. (2006) Identification of the Allosteric Amino Acid Binding Site in Mammalian Pyruvate Kinase at 1.6Å. 50th Annual Biophysical Society Meeting, Salt Lake City, UT. 16. Williams, R., Pendergrass, D. C., Blair, J. B., and Fenton, A. W. (2005) Data Mining for Information on Allostery in Pyruvate Kinase Isozymes. Kansas City Area Life Sciences Research Day, Kansas City, KS. 17. Fenton, A. W. (2005) Differentiating the Chemical Requirements for Ligand-Induced Allosteric Inhibition from those of Ligand Binding: Phenylalanine Inhibition of Pyruvate Kinase in Phenylketonuria. Kansas City Area Life Sciences Research Day, Kansas City, KS. 18. Fenton, A. W. and McDonald, G. (2005) Differentiating the Chemical Requirements for Ligand-Induced Allosteric Inhibition from those of Ligand Binding; Phenylalanine Inhibition of Pyruvate Kinase. 19th Annual Gibbs Conference on Biothermodynamics, Carbondale, Il. 19. Fenton, A. W. (2005) Differentiating the Chemical Requirements for Ligand-Induced Allosteric Inhibition from those of Ligand Binding; Phenylalanine Inhibition of Pyruvate Kinase. 49th Annual Biophysical Society Meeting, Long Beach, CA. 20. Fenton, A. W., Paricharttanakul, N. M., and Reinhart, G. D. (2003) Allosteric Inhibition and Activation in E. coli Phosphofructokinase use the Same Binding Sites but Different Pathways. 47th Annual Biophysical Society Meeting, San Antonio, TX. 21. Fenton, A. W. and Reinhart, G. D. (2003) Hybrid Tetramers Reveal the Substrate Inhibition of E. coli Phosphofructokinase to be an Allosteric Mechanism, NOT a Kinetic Mechanism. 18th Enzyme Mechanisms Conference, Galveston Island, TX. 22. Fenton, A. W. and Reinhart, G. D. (2002) Binding Energy is Not Conferred by Binding Site Residues Alone; the Binding Site of E. Coli Phosphofructokinase. 46th Annual Biophysical Society Meeting, San Francisco, CA. 23. Fenton, A. W. and Reinhart, G. D. (2001) Quantification of Isolated Heterotropic Interactions Contributing to Allostery of Phosphofructokinase from E. coli. 6th Annual Structural Biology Symposium, Sealy Center for Structural Biology, Galveston, TX. 24. Fenton, A. W. and Reinhart, G. D. (2001) Quantification of Isolated Heterotropic Interactions Contributing to Allostery in Phosphofructokinase from E. coli. 45th Annual Biophysical Society Meeting, Boston, MA. 25. Fenton, A. W. and Reinhart, G. D. (2000) Formation of a Modified

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Tetramer of E. coli Phosphofructokinase with Only One Native Active Site and One Native Allosteric Site. 14th Protein Society Symposium, San Diego, CA.

Invited Scientific Presentations

ACS-Kansas City chapter guest speaker to class of 2012 student honorees

St. Marys University

Allosteric Regulation of Human Liver Pyruvate Kinase, Spring 2012

University of North Carolina-Charlotte, Department of Bioinformatics and Genomics

Allosteric Regulation of Human Liver Pyruvate Kinase, Fall 2011

University of Missouri, Department of Chemistry and Biochemistry

Allosteric Regulation of Human Liver Pyruvate Kinase, Spring 2011

Pittsburg State University, Department of Chemistry

Regulation of human liver pyruvate kinase by N-terminal modification, Fall 2010

Oklahoma State University, Department of Biochemistry and Molecular Biology

Allosteric Regulation of Pyruvate Kinase, Fall 2009

Notre Dame, Department of Chemistry

Allosteric Regulation of Pyruvate Kinase, Fall 2009

The University of Minnesota Duluth, Department of Chemistry and Biochemistry

Allosteric Regulation of Pyruvate Kinase, Fall 2009

Western Missouri State, Chemistry Department,

Allosteric Regulation of Pyruvate Kinase, Fall 2008 Kansas University, Department of Medicinal Chemistry, Allosteric Regulation of Pyruvate Kinase, Spring 2008 Southwestern Oklahoma State University, Chemistry Department,

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Allosteric Regulation of Pyruvate Kinase, Spring 2008 Pittsburg State University, Department of Chemistry, Differentiating a Ligand’s Chemical Requirements for Allosteric Interactions from Those for Protein Binding: Phenylalalnine Inhibition of Pyruvate Kinase, Fall 2006 Missouri State University, Department of Biomedical Sciences, Differentiating a Ligand’s Chemical Requirements for Allosteric Interactions from Those for Protein Binding: Phenylalalnine Inhibition of Pyruvate Kinase, Spring 2006 Wichita State University, Biology Department, “Characterizing the Allosteric Amino Acid Effector of Pyruvate Kinase, Spring 2005 Kansas University, Chemistry Department, “Characterizing the Allosteric Amino Acid Effector of Pyruvate Kinase, Spring 2005 University of Missouri-Kansas City, School of Biological Sciences, Allosteric Regulation in Glycolytic Enzymes, Fall 2004

Talks at Scientific Meetings

Biophysical Society Meeting 2013, Philadelphia, PA

Energetic coupling between an oxidizable cysteine and the phosphorylatable N-terminus of human liver pyruvate kinase

Keystone Symposia on Allosteric Regulation 2009, Keystone, CO

Mapping Individual residues within the N-terminus that are energetically coupled to allosteric and active site functions of human liver pyruvate kinase

Reviewing for Scientific Journals

Science, Reviewer PNAS, Reviewer Journal of Molecular Biology, Reviewer Journal of Biological Chemistry, Reviewer Biochemistry, Reviewer Biophysical Journal, Reviewer Journal of the American Society for Mass Spectrometry, Reviewer Biochemistry Journal, Reviewer Archives of Biochemistry and Biophysics, Reviewer Proteins: Structure, Function and Bioinformatics, Reviewer

Grant Peer Review

NSF Outside Reviewer

Meetings - 2013 (scheduled for Oct. 2013) Gibbs Conference in

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Organized Biothermodynamics, an invitation only meeting

2013 (scheduled for Nov. 2013) The Heartland Undergraduate Biochemistry (HUB) Forum, Kansas City Kansas, guest lecturer Michael Marletta 2012 The Heartland Undergraduate Biochemistry (HUB) Forum, Kansas City Kansas, guest lecturer Heidi Hamm 2011 The Heartland Undergraduate Biochemistry (HUB) Forum, Kansas City Kansas, guest lecturer Richard Hanson

Meetings – assigned duties

2013 Biophysical Society Meeting, Philadelphia, PA – Session Chair

2012 Gordon Conference on Enzymes, Coenzymes and Metabolic Pathways, Waterville Valley, NH. – Session Chair

Funding Current: RO1 DK78076 2/19/08-1/30/14 NIH/NIDDK Role: P.I. The goal of this project is to characterize the mechanism of allosteric regulation in human liver pyruvate kinase, the regulation of which is vital to controlling blood glucose. Current: NIH vis KUMC 7/1/12-6/30/13 University of Kansas Alzheimer’s Disease Center – Pilot Project Role: P.I. of Pilot Project The goal of this project is to determine if the cancer associated changes in isozyme expression of pyruvate kinase and covalent modifications of this protein that are associated with cell cycle re-entry are also present in Alzheimer’s brain. Past: P20 RR16475 (PI: Hunt, J.S.) 1/1/04-6/30/04 NIH/NCRR BRIN Role: K-BRIN recruitment package recipient The goal of this project was to characterize what regions of Phe are important for eliciting allosteric inhibition in pyruvate kinase from rabbit muscle.

Continued Education

Grant Writers’ Seminars and Workshops, LLC., 2004 COBRE Symposium on Protein Structure and Function (University of Kansas, Lawrence, KS), 2004

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Curriculum Vitae HARVEY F. FISHER

Professor of Biochemistry Director, Laboratory of

University of Kansas and Molecular Biochemistry

School of Medicine Department of Veterans

Affairs Medical Center

4801 Linwood Blvd.

Kansas City, MO 64128-2295

EDUCATION: 1940-1943 Major - Chemistry, Western Reserve University, Cleveland,

Ohio

1946-1947 B.S. - Western Reserve University, Cleveland, Ohio

1947-1952 Ph.D. - University of Chicago, Chicago, Illinois

PRECEPTORS: Professor Frank H. Westheimer

Professor Birgit Vennesland

THESIS TITLE: The Enzymatic Transfer of Hydrogen

POST GRADUATE POSITIONS: 1952-1954 Postdoctoral Fellow, Coll. Phys. and Surg., Columbia

University, New York, New York (Prof. David Rittenberg)

1954-1956 Project Associate, Department of Chemistry, University

of Wisconsin, Madison, Wisconsin (Prof. Robert Alberty)

1956-1957 Instructor of Chemistry, Department of Chemistry,

University of Massachusetts, Amherst, Massachusetts

1957-1963 Senior Associate, Edsel B. Ford Institute for Medical

Research, Detroit, Michigan

1963-1965 Associate Professor, University of Kansas Medical Center

Kansas City, Kansas

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1963-present Director, Laboratory of Molecular Biochemistry, Department of

Veterans Affairs Medical Center, Kansas City, Missouri

1965-present Professor of Biochemistry, University of Kansas Medical Center

Kansas City, Kansas

1997-present Adjunct Professor, School of Life Sciences, U. Missouri, Kansas

City, Missouri

RESEARCH INTERESTS:

1. Mechanism of Enzymatic Catalysis

2. Structure-function Relationships in Macromolecules

3. Thermodynamics of Biological Interactions, including Steady State-System and

Cooperative Effects

AWARDS AND FELLOWSHIPS:

1951-1952 Atomic Energy Commission Predoctoral Fellow

1955 (Declined) Damon Runyon Cancer Fellow

1961 Recipient of National Science Foundation Travel Award from

American Society of Biological Chemists to present paper

before the Fifth International Biochemical Congress,

Moscow, USSR

1967 Recipient of an award from the American Society of Biological

Chemists for travel to the Seventh International Congress

of Biochemists, Tokyo, Japan

1978 Veterans Administration Career Scientist Award

GRANT SUPPORT:

1962-1986 NSF (The Energetics of Enzyme Catalyzed Reactions)

1968-1993 NIH (Mechanisms of Enzymatic Catalysis)

1964-1997 VA National Peer Reviewed Support (Structure-Function Relationships in Bacterial Glutamate Dehydrogenase - $133,100 support this year)

1987 BRSG (Energetics of Enzyme-Ligand Interactions)

1990-1992 NSF (Support for Symposium on "The Thermodynamic Basis of

Protein Structure & Function)

1992-1995 NIH (Binding Energy Transduction in Enzymatic

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Catalysis)

1993-1996 NSF (Mechanisms of Enzymatic Catalysis)

1996-1999 NSF (Mechanisms of Enzymatic Catalysis - $71,429 support this

year)

1996 NSF (Supplement to Mechanisms of Enzymatic Catalysis,

Research Experience for Undergraduates - $3,260 support)

1997-1999 NSF (Supplement to Mechanisms of Enzymatic Catalysis,

Research Experience for Undergraduates - $4,000 support)

MEMBERSHIP IN PROFESSIONAL SOCIETIES:

American Society of Biological Chemists

Sigma Xi

AAAS

Biophysical Society

American Chemical Society

MEMBERSHIP ON NATIONAL & INTERNATIONAL COMMITTEES AND EDITORIAL BOARDS:

Veterans Administration Liaison Representative to NIH Biochemistry Study Section

(1965-1968)

Member, Veterans Administration National Basic Science Advisory Panel (1966-1968)

Member, Committee of Isoenzymes and Enzyme Subunits Established by the

Commission of Editors of Biochemical Journals of the International Union of

Biochemistry.

Member, Veterans Administration Biochemistry-Biophysics Research Evaluation

Committee (1969-1971)

Member, Veterans Administration National Merit Review Board for Basic Sciences

(1981-1984)

National Correspondent, Public Policy Committee, American Society of Biological

Chemists, Division of Biological Chemistry, American Chemical Society

Chairman, Subcommittee on Extramural Grants for VA Research Established by the

Research and Education Council of the Veterans Administration

Member, Editorial Board, The Journal of Biological Chemistry (1973-1980)

Member, National Science Foundation Molecular Biology Advisory Panel (1974-1977)

Member, Editorial Board, Archives of Biochemistry and Biophysics (1981- )

Invited Expert, Editorial Board, Chemtracts (1992 - )

NSF Multi-User Instrument Panel (1996-1997)

Ad Hoc Member, FASEB Public Affairs Committee (1997)

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SYMPOSIA PARTICIPATION:

Symposium on the Mechanism of Action of the Pyridine Nucleotide-Dependent

Dehydrogenases in Honor of Hugo Theorell, University of Kentucky, (Invited

Speaker), March 16-19, 1965.

Seventh International Congress of Biochemistry, Tokyo, Japan (Invited Speaker),

August 19-25, 1967.

Symposium on Molecular Conformation of Proteins in Solution at the meeting of the

Division of Biological Chemistry of the American Chemical Society, Atlantic City,

New Jersey, (Chairman, Sept. 8-13, 1968).

Second Conference on Enzyme Mechanisms, Santa Barbara, California, (Invited

Speaker), December 16-29, 1970.

Intra-Science Symposium on the mechanisms of Enzyme Action, Santa Monica,

California (Invited Speaker), December 2-3, 1971.

Conference on Techniques of Microcalorimetric Investigations of Cellular Systems

with Special Reference to the Clinical Field, Lund, Sweden, (Session Chairman),

July 9-11, 1973.

International Symposium on New Applications of Microcalorimetry as Applied in

Biomedical Research, Bethesda, Maryland, (Invited Speaker), January 28-29, 1976.

Frank H. Westheimer Symposium on Enzymes, Cincinnati, Ohio, (Invited Speaker),

October 22, 1976.

Biochemistry Present and Future, Twelfth Midwest American Chemical Society

Meeting, Kansas City, Missouri, (Chairman), October 28-29, 1976.

International Symposium on Mechanisms of Oxidizing Enzymes, La Paz, Baja,

California Sur, Mexico, (Invited Speaker), December 5-7, 1977.

Symposium on Electron Transfer and Interfacial Behavior of Molecules by

Biological Importance, Electrochemical Society Meeting, St. Louis, Missouri

(Invited Speaker), May 13-14, 1980.

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IUPAC Conference on Chemical Thermodynamics/The 39th Annual Calorimetry

Conference, Hamilton, Canada, (Invited Speaker), August 13-17, 1984.

Watkins Midwest Biophysics and Biophysical Chemistry Conference, Wichita,

Kansas (Invited Speaker), October 3-4, 1986.

International Symposium on Thermodynamics Applied to Biological Systems,

Santa Margherita Ligure, Italy, September 11-17, 1988.

Molecular Mechanisms of Protein Recognition, Kyoto, Japan (Invited Speaker),

March 12-16, 1989.

International Symposium in Honor of Rufus Lumry, "The Thermodynamic Basis of Protein Structure and Function", Kansas City, Missouri (Local Chairman),

October 4-6, 1990.

Workshop on Transmembrane Electric Fields, Elkridge, Maryland (Invited Speaker)

October 23-26, 1990.

National Meeting of the Biophysical Society, February 1998, Kansas City (Local Chairman)

Co-Chairman and speaker, International Symposium on Mechanisms of Enzymatic Catalysis, Guilin, China (May 1998)

UNDERGRADUATE SCIENCE MENTORING

Science Pioneers (1991 - )

Ryan McCue, Summer Research Project (1990)

Peter Meyerdirk, “Unique Academic Project” and visit to lab (1995)

Chad Brocker, Shadow-ship Program (1995)

UMKC High School Science, Mathematics and Technology Institute, Phase II, tour of

laboratory (1997)

Robert Pierce, David Weiss, Lawrence Indyk, David Kristensen NSF Research

Experience for Undergraduates (1996-1998)

MAJOR PUBLICATIONS

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1. F. H. Westheimer, H.F. Fisher, E.E. Conn and B. Vennesland. The Enzymatic Transfer of

Hydrogen from Alcohol to DPN. J. Am. Chem. Soc., 73:2403, 1951.

2. H.F. Fisher, E.E. Conn, B. Vennesland and F.H. Westheimer. The Enzymatic Transfer of Hydrogen. I. The Reaction Catalyzed by Alcohol Dehydrogenase. J. Biol. Chem., 202:687, 1953.

3. F.A. Loewus, P. Ofner, H.F. Fisher, F.H. Westheimer and B. Vennesland. The Enzymatic Transfer to Hydrogen. II. The Reaction Catalyzed by Lactic Dehydrogenase. J. Biol. Chem., 202:699, 1953.

4. H.F. Fisher, A.I. Krasna and D. Rittenberg. The Interaction of Hydrogenase with Oxygen. J. Biol. Chem., 209:569, 1954.

5. H.F. Fisher, C. Frieden, J.S. McKinley McKee and R.A. Alberty. Concerning the Stereospecificity of the Fumarase Reaction and the Demonstration of a New intermediate. J. Am. Chem. Soc., 77:4436, 1955.

6. R.A. Alberty, W.G. Miller and H.F. Fisher. Studies on the Enzyme Fumarase. VI. The Incorporation of Deuterium into L-Malate During the Reaction in Deuterium Oxide. J. Am. Chem. Soc., 79:3973, 1957.

7. H.F. Fisher and L.L. McGregor. On the Nature of the Fluorescence of Enzyme-DPNH Complexes. Biochim. Biophys. Acta, 38:562, 1960.

8. H.F. Fisher. The Mechanism of the Glutamic Dehydrogenase Reaction. I. The Molecularity of the First Complex Formed. J. Biol. Chem., 235:1830, 1960.

9. H.F. Fisher and L.L. McGregor. The Nature of the Fluorescence of an Enzyme-Reduced Coenzyme-Reduced Substrate Complex. Biochim. Biophys Acta, 43:557, 1960.

10. H.F. Fisher and L.L. McGregor. The Role of the Ammonium Moiety in the Glutamic Dehydrogenase Reaction. Biochem. Biophys. Res. Commun., 3:629, 1960.

11. H.F. Fisher and L.L. McGregor. The Mechanism of the Glutamic Dehydrogenase Reaction. II. Substrate Specificity of the Enzyme. J. Biol. Chem., 236:791, 1961.

12. H.F. Fisher. The Appearance of Substrate Binding Terms in the Vmax Expression of the Enzyme Reactions. J. Theoret. Biol., 3:484, 1962.

13. H.F. Fisher, L.L. McGregor and U. Power. The Nature of the Alkaline Dissociation of the Glutamic Dehydrogenase Molecule. Biochem. Biophys. Res. Commun., 8:402, 1962.

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14. H.F. Fisher, L.L. McGregor land D.G. Cross. The Role of Tyrosyl-Hydrogen Bonds in the

Quaternary Structure of the Glutamic Dehydrogenase Molecule. Biochim. Biophys. Acta, 65:175, 1962.

15. H.F. Fisher, D.G. Cross and L.L. McGregor. Catalytic Activity of Subunits of Glutamic Dehydrogenase. Nature, 196:895, 1962.

16. H.F. Fisher. A Nomenclature for the Quaternary Structure of an Enzyme Molecule. Nature, 198:665, 1963.

17. H.F. Fisher. A Limiting Law Relating the Size and Shape of Protein Molecules to Their Composition. Proc. Nat. Acad. Sci., 51:1285-1291, 1964.

18. H.F. Fisher and D.G. Cross. Spectrophotometric Studies of the Quaternary Structure of Proteins. I. The Method of Concentration-Difference Spectra. Archives of Biochem. and Biophys., 110:217-221, 1965.

19. D.G. Cross and H.F. Fisher. Spectrophotometric Studies of the Quaternary Structure of Proteins. II. The , Split of Glutamic Dehydrogenase. Archives of Biochem. and Biophys., 110:222-226, 1965.

20. H.F. Fisher, D.G. Cross and L.L. McGregor. The Independence of the Substrate Specificity of Glutamic Dehydrogenase on its State of Aggregation. Biochim. Biophys. Acta, 99:165-167, 1965.

21. H.F. Fisher and D.G. Cross. The Involvement of Tryptophan Residue of Glutamate Dehydrogenase in the Binding of L-Glutamate. Biochem. Biophys. Res. Commun., 20:120-123, 1965.

22. H.F. Fisher, An Upper Limit to the Amount of Hydration of a Protein Molecule. A Corollary to the "Limiting Law of Protein Structure". Biochim. Biophys. Acta, 109:544-550, 1965.

23. W.C. McCabe and H.F. Fisher. Measurement of the Excluded Volume of Protein Molecules by Differential Spectroscopy in the Near Intra-Red. Nature, 207:1274-1276, 1965.

24. D.G. Cross and H.F. Fisher. The Spatial Location of Chromophoric Residues in L-Glutamate Dehydrogenase. Biochemistry, 5:880-885, 1966.

25. H.F. Fisher and D.G. Cross. Enzyme-Coenzyme Complexes of Pyridine Nucleotide-Linked Dehydrogenases. Science, 153:414-415, 1966.

26. H.F. Fisher. Geometrical Considerations for the Theory of Protein Structure - Symposium on Higher Order Structures in Biopolymers - Seventh International Congress of Biochemistry, Tokyo, 1967.

116

27. H.F. Fisher and L.L. McGregor. The Ability of Reduced Nicotinamide Mononucleotide to

Function as a Hydrogen Donor in the Glutamic Dehydrogenase Reaction. Biochem. Biophys. Res. Commun., 34:627-632, 1969.

28. D.G. Cross, and H.F. Fisher. Conformation and Conformational Changes of Reduced Diphosphospyridine Nucleotide in Solution. Biochemistry, 8:1147-1155, 1969.

29. H.F. Fisher and J.R. Bard. The Kinetic Measurement of the - Dissociation of Glutamate Dehydrogenase. Biochim. Biophys. Acta, 188:168-170, 1969.

30. H.F. Fisher, D.L. Adija and D.G. Cross. Dehydrogenase-Reduced Coenzyme Difference Spectra, Their Resolution and Relationship to the Stereospecificity of Hydrogen Transfer. Biochemistry, 8:4424-4431, 1969.

31. H.F. Fisher and J.R. Bard. An Intermediate in the pH-Induced Dissociation of Glutamate Dehydrogenase. Biochem. Biophys. Res. Commun., 37:581-586, 1969.

32. H.F. Fisher. "The Structure of the Glutamic Dehydrogenase Molecule and Its Subunits." in The Mechanism of Action of Dehydrogenases, eds., G.W.Schwert and A.D. Winer. The University Press of Kentucky, 223-253, 1970.

33. W.C. McCabe and H.F. Fisher. A Near-Infrared Spectroscopic Method for Investigating the Hydration of a Solute in Aqueous Solution. J. Phys. Chem., 74:2990-2998, 1970.

34. D.G. Cross and H.F. Fisher. The Mechanism of Glutamate Dehydrogenase Reaction. III. The Binding of Ligands at Multiple Subsites and Resulting Kinetics Effects. J. Biol. Chem., 245:2612-2621, 1970.

35. W.C. McCabe, S. Subramanian and H.F. Fisher. A Near-Infrared Spectroscopic Investigation of the Effect of Temperature on the Structure of Water. J. Phys. Chem., 74:4360-4369, 1970.

36. H.F. Fisher, R.E. Gates and D.G. Cross. A Ligand Exclusion Theory of Allosteric Effects. Nature, 228:247-249, 1970.

37. H.F. Fisher, J.R. Bard and R.A. Prough. Transient-State Intermediates Involved in the Hydride Transfer Step of the Glutamate Dehydrogenase Reaction. Biochem. Biophys. Res. Commun., 41:601-607, 1970.

38. S. Subramanian and H.F. Fisher. Splitting of Water Absorption Band by Perchlorate Anion - Assignment of High Frequency Band. Roczniki Chemii, 45:933-935, 1971.

39. A.M. Fiskin, E.F.J. van Bruggen and H.F. Fisher. Structure and Function of Oligomeric Dehydrogenases. Electron Microscopic Studies of Beef Liver Glutamate Dehydrogenase Quaternary Structure. Biochemistry, 10:2396-2408, 1971.

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40. R.E. Gates and H.F. Fisher. The Restrictions of Sequence on the Thickness of Globular Protein Molecules. Proc. Nat. Acad. Sci., 68:2928-2931, 1971.

41. S. Subramanian and H.F. Fisher. Detection of Polar Side Chain Hydration in Polypeptides by Near-Infrared Spectroscopy. Biopolymers, II:1305-1309, 1972.

42. S. Subramanian and H.F. Fisher. Near-Infrared Spectral Studies on the Effects of Perchlorate and Tetrafluoroborate Ions on Water Structure. J. Phys. Chem. 76:84-89, 1972.

43. H.F.Fisher, J.M. Culver and R.A. Prough. The Independence of Adenosine-5 -Diphosphate Binding and the State of Association of L-Glutamate Dehydrogenase. Biochem. Biophys. Res. Commun., 461:462-1468, 1972.

44. R. A. Prough, J.M. Culver and H.F. Fisher. Spectrophotometric Evidence for a Glutamate Dehydrogenase--L-Leucine Complex. Arch. Biochem. Biophys., 149:414-418, 1972.

45. R.A. Prough and H.F. Fisher. Specificity and Spectral Resolution of an L-Glutamate Dehydrogenase-Nonocarboxylic Amino Acid Complex. Biochemistry, 11:2479-2482, 1972.

46. A.H. Colen, R.A. Prough and H.F. Fisher. The Mechanism of Glutamate Dehydrogenase Reaction. IV. Evidence for Random and Rapid Binding of Substrate and Coenzyme in the Burst Phase. J. Biol. Chem., 247:7905-7909, 1972.

47. D.G. Cross, L.L. McGregor and H.F. Fisher. The Binding of -ketoglutarate in a Binary Complex and in a Ternary Complex with NADP+ by L-Glutamate Dehydrogenase. Biochim. Biophys. Acta, 289:28-36, 1972.

48. H.F. Fisher. "Glutamate Dehydrogenase--Ligand Complexes and Their Relationship to the Mechanism of the Reaction," in Advances in Enzymology, ed., A. Meister, Interscience Publishers, New York, 369-417, 1973.

49. H.F.Fisher and D.G. Cross. "Ligand Exclusion As An Alternate Theory of Allosteric Effects," in Intra-Science Chemistry Reports, ed., N. Kharasch, Intra-Science Research Foundation, Vol. 6, pp. 61-71, 1972.

50. R.A. Prough, A.H. Colen and H.F. Fisher. Spectrophotometric Observation of a Glutamate Dehydrogenase-L-Glutamate Complex. Biochim. Biophys. Acta, 284:16-19, 1972.

51. A. Brown, J.M. Culver and H.F.Fisher. Mechanism of Inactivation of L-Glutamate Dehydrogenase by Pyridoxal and Pyridoxal Phosphate. Biochemistry, 12:4367-4373, 1973.

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52. R.A. Prough, J.M. Culver and H.F. Fisher. The Mechanism of Activation of Glutamate

Dehydrogenase-Catalyzed Reactions by Two Different Cooperatively Bound Activators. J. Biol.Chem., 248:8528-8533, 1973.

53. A.H. Colen, D.G. Cross and H.F.Fisher. Two-Step Binding of Adenosine Diphosphate to L-Glutamate Dehydrogenase. Effect on Binding of Reduced Nicotinamide Adenine Diphosphate and on Enzymatic Catalysis. Biochemistry, 13:2341-2347, 1974.

54. A.H. Colen, R.R. Wilkinson and H.F.Fisher. Location of Deuterium Oxide Solvent Isotope Effects in the Glutamate Dehydrogenase Reaction. J. Biol. Chem., 250:5243-5246, 1975.

55. D.G. Cross, A. Brown, and H.F. Fisher. Hydrogen-Deuterium Exchange in Nucleosides and Nucleotides. A Mechanism for Exchange of the Exocyclic Amino Hydrogens of Adenosine. Biochemistry, 14:2745-2749, 1975.

56. S. Subramanian, D.C. Stickel and H.F. Fisher. Thermodynamics of Complex Formation Between Bovine Liver Glutamate Dehydrogenase and Analogs of ADP. J. Biol. Chem., 250:5885-5889, 1975

57. D.G. Cross, A. Brown and H.F. Fisher. Hydrogen Exchange at the Amide Group of Reduced Pyridine Nucleotides and the Inhibition of that Reaction by Dehydrogenases. J. Biol. Chem., 251:1785-1788, 1976.

58. A. Brown and H.F. Fisher. A Comparison of the Glutamate Dehydrogenase Catalyzed Oxidation of NADPH by Trinitrobenzene Sulfonate with the Uncatalyzed Reaction. J. Amer. Chem. Soc., 98:5682-5688, 1976.

59. A.H. Colen, R.R. Wilkinson and H.F. Fisher. The Transient-State Kinetics of L-Glutamate Dehydrogenase: pH Dependence of the Burst Rate Parameters. Biochim. Biophys. Acta, 481:377-383, 1977.

60. H.F. Fisher, D.C.Stickel, A. Brown and D. Cerretti. Determination of the Thermodynamic Parameters of Individual Steps of the Pyruvate-Oxime Formation by Rapid, Continuous Flow Microcalorimetry, J. Amer. Chem.Soc., 99:8180-8182, 1977.

61. A. Brown, A.H. Colen and H.F. Fisher. Effect of Ammonia on the Glutamate Dehydrogenase Catalyzed Oxidative Deamination of L-Glutamate: I. Production of an Ammonia-Containing Intermediate in the "Burst" Phase. Biochemistry, 17:2031-2034, 1978.

62. H.F. Fisher and A.H. Colen. "The Mechanism and Energetics of the Glutamate Dehydrogenase Reaction," in Developments in Biochemistry: II. Mechanisms of Oxidizing Enzymes. eds., T.P. Singer and R.N. Ondarza, Elsevier North Holland, Inc., 95-108, 1978.

63. S. Subramanian, D. Stickel, A.H. Colen and Harvey F. Fisher. Thermodynamics of Heterotropic Interactions. The Glutamate Dehydrogenase-NADPH-Glutamate Complex. J. Biol. Chem., 253:8369-8374, 1978.

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64. B.A. Bradley, A.H. Colen and H.F. Fisher. The Effects of Methanol on the Glutamate Dehydrogenase Reaction at 0 C. Biophys. J., 25:555-561, 1979.

65. A. Brown, A.H. Colen and H.F. Fisher. Effect of Ammonia on the Glutamate Dehydrogenase Catalyzed Oxidative Deamination of L-Glutamate. II. The Steady State. Biochemistry, 18:5924-5928, 1979.

66. H.F. Fisher, S. Subramanian, D. Stickel, and A.H. Colen. The Thermodynamics of a Negatively Interacting Allosteric Effector System: The Glutamate Dehydrogenase-NADPH-ADP Complexes. J. Biol. Chem., 255:2509-2513, 1980.

67. H.F. Fisher and D.C. Stickel. Thermodynamic Interaction Parameters of a Reactive Enzyme Ternary Complex: Glutamate Dehydrogenase--NADPH--Ketoglutarate. FEBS Letters, 113:11-14, 1980.

68. H.F. Fisher, D.C. Stickel and A.H.Colen. pH Dependent Thermodynamic Parameters of the Glutamate Dehydrogenase--Ketoglutarate-NADPH Complex. Biochim. Biophys. Acta, 615:27-33, 1980.

69. R.E. Johnson, P.J. Andree and H.F. Fisher. Cryoenzymological Studies of the Oxidative Deamination of L-Glutamate by Glutamate Dehydrogenase: Spectral Resolution of Transient and Product Complexes. J. Biol. Chem., 256:3817-3821, 1981.

70. R.E. Johnson, P.J. Andree and H.F. Fisher. Evidence for a Change in the Structure of Glutamate Dehydrogenase During its Catalytic Cycle Under Cryogenic Conditions. J. Biol. Chem., 256:6381-6384, 1981.

71. A.H. Colen, R.T. Medary and H.F. Fisher. Transient Phase Energetics of the Oxidative Deamination of L-Glutamate by L-Glutamate Dehydrogenase and NADP: A Reaction with a Large Negative Heat Capacity of Activation. Biopolymers, 20:879-889, 1981.

72. H.F. Fisher, A.H. Colen and R.T. Medary. Temperature-Dependent Cp Generated by a Shift in Equilibrium Between Macrostates of an Enzyme. Nature, 292:271-272, 1981.

73. R. Srinivasan, R.T. Medary, H.F. Fisher, D.J. Norris and R. Stewart. The Pyridinium-Dihydropyridine System. Reduction Potentials and the Mechanism of Oxidation of 1,4-Dihydropyridines by a Schiff Base. J. Am.Chem. Soc., 104:807-812, 1982.

74. T.S. Viswanathan, R.E. Johnson and H.F. Fisher. -Ketoglutaric Acid-Solution Structure and the Active Form for Reductive Amination by Bovine Liver Glutamate Dehydrogenase. Biochemistry, 21:339-345, 1982.

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75. T.S. Viswanathan, C.E. Hignite and H.F. Fisher. Determination of Carbonyl Oxygen Exchange

Rates in -Ketoacids by Gas Chromatography-Mass Spectrometry. Analytical Biochemistry, 123:295-302, 1982.

76. H.F. Fisher, R. Srinivasan and A.E. Rougvie. Glutamate Dehydrogenase Catalyzes the Reduction of A Schiff Base, -Pyrroline-2-Carboxylic Acid, by Reduced Pyridine Dinucleotide. J. Biol. Chem., 257:13208-13210, 1982.

77. A.H. Colen, R.E. Johnson and H.F. Fisher. The Effect of Cryosolvent on the Transient Kinetics of the Glutamate Dehydrogenase Reaction. Biochemistry, 21:6695-6699, 1982.

78. R. Srinivasan and H.F. Fisher. Configurational, Conformational and Solvent Effects on the Reduction of a Schiff Base by Reduced Pyridine Nucleotide Analogs. Arch. Biochem. Biophys., 223:453-457, 1983.

79. H.F. Fisher. "L-Glutamate Dehydrogenase from Bovine Liver" in Methods in Enzymology, ed., A. Meister, Academic Press, New York, 16-27, 1985.

80. H.F. Fisher, R.T. Medary, E.J. Wykes and C.S. Wolfe. Thermodynamic Interactions in the Glutamate Dehydrogenase-NADPH-Oxalylglycine Complex. J. Biol. Chem., 259:4105-4110, 1984.

81. T.S. Viswanathan and H.F. Fisher. Carbonyl Oxygen Exchange Evidence of Imine Formation in Glutamate Dehydrogenase Reaction and Identification of the "Occult Role" of NADPH. Proc. Natl. Acad. Sci., 81:2747-2751, 1984.

82. R. Srinivasan and H.F. Fisher. The Reversible Reduction of an -imino Acid to -amino Acid Catalyzed by Glutamate Dehydrogenase: The Effect of Ionizable Function Groups. Biochemistry, 24:618-622, 1985.

83. R. Srinivasan and H.F. Fisher. A Comparison of the Energetics of the Spontaneous and the Glutamate Dehydrogenase Catalyzed -Imino Acid---Amino Acid Interconversion. Biochemistry, 24:5356-5360, 1985.

84. R. Srinivasan and H.F. Fisher. Deuterium Isotope Effects for the Nonenzymatic and Glutamate Dehydrogenase-Catalyzed Reduction of an -imino Acid by NADH. J. Am. Chem. Soc., 107:4801-4805, 1985.

85. H.F. Fisher, S. Maniscalco, C. Wolfe, land R. Srinivasan. NADPH-Binding-Induced Proton Ionization as a Cause of Non-Linear Heat Capacity Changes in Glutamate Dehydrogenase. Biochemistry, 25:2910-2915, 1986.

86. P. Chalabi, S. Maniscalco, L.E. Cohn, and H.F. Fisher. The Effects of an Acetate Sensitive Anion Binding Site on NADPH Binding in Glutamate Dehydrogenase. Biochim. Biophys. Acta, 913:103-110, 1987.

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87. R. Srinivasan and H.F. Fisher. Structural Features Facilitating the Glutamate Dehydrogenase Catalyzed -imino Acid -amino Acid Interconversion. Arch. Biochem. Biophys., 246:743-750, 1986.

88. H.F. Fisher. "A Unifying Model of the Thermodynamics of Formation of Dehydrogenase-ligand Complexes" in Advances in Enzymology, Vol. 61, ed. A. Meister, John Wiley and Sons, 1-46, 1988.

89. H.F.Fisher, S. Pazhanisamy, R.T. Medary. The Anomalous Properties of the Glutamate Dehydrogenase--NADPH---Ketoglutarate Complex are not Ascribable to Carbonyl Addition Reactions. J. Biol. Chem., 262:11684-11687, 1987.

90. R. Srinivasan, T.S. Viswanathan, and H.F. Fisher. Mechanism of Formation of Bound -Iminoglutarate from -Ketoglutarate in the Glutamate Dehydrogenase Reaction. J.Biol. Chem., 263:2304-2308, 1988.

91. H.F. Fisher, S. Maniscalco, N. Singh, S.A. Adediran. Proton/Product Time Course Ratios: A New Approach to Transient-State Kinetic Analysis. J. Biol. Chem., 263:11704-11710, 1988.

92. H.F. Fisher and N. Singh. The Meaning of Interaction Parameters in Two-State Protein Complexes. J. Biol. Phys., 17:213-220, 1990.

93. H.F. Fisher and N. Singh. Transduction of Enzyme-Ligand Binding Energy into Catalytic Driving Force. FEBS Letters, 294:1-5, 1991.

94. H.F. Fisher, S. Maniscalco, N. Singh, R.N. Mehrotra, and R. Srinivasan. A Slow Obligatory Proton Release Step Precedes Hydride Transfer in the Liver Glutamate Dehydrogenase Catalytic Mechanism. Biochim. Biophys. Acta, 1119:52-56, 1992.

95. N. Singh, H.F. Fisher, and R.N. Mehrotra. Resolution of Rate Constants in a two-Step Binding Process with a Single Observable Relaxation. J. Phys. Chem., 96:5688-5689, 1992.

96. H.F. Fisher. (J.M. Sanchez-Ruiz, Theoretical Analysis of Lumry-Eyring Models in Differential Scanning Calorimetry, Biophys. J., 61:921, 1992) Reviewed in Chemtracts 3:313-318 (1992).

97. N. Singh, S. Maniscalco, and H.F. Fisher. The Real-Time Resolution of Proton-Related Transient-State Steps in an Enzymatic Reaction. J. Biol. Chem., 268:21-28 (1993)

98. H.F. Fisher and N. Singh. "Calorimetric Methods for Interpreting Protein-Ligand Interactions." in Methods in Enzymology, Vol. 259, eds. G.K. Ackers and M.L. Johnson, Academic Press, Inc., 194-221, 1995.

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99. N. Singh and H.F. Fisher. Protein-ligand Interactions as a Driving Force for a High-enthalpy

Two-state Transition in Glutamate Dehydrogenase: The Opposing Roles of Phosphate and Acetate Ions. Arch. Biochem. Biophys, 312:38-44 (1994).

100. S. Pazhanisamy, S.J. Maniscalco, N. Singh, and H.F. Fisher. A Kinetic Mechanism of the Allosteric Control of Enzyme-coenzyme Binding: Glutamate Dehydrogenase-NADPH-Phosphate-Acetate-Hydrogen Ion Interactions. Biochemistry , 33:10381-10385 (1994).

101. S.K. Saha, S.J. Maniscalco, N. Singh, H.F. Fisher. The Demonstration of a Glutamate Dehydrognease--NADP-L-Glutamate Charge-transfer Complex and Its Location on the Reaction Pathway. J. Biol. Chem. 269:29592-29597 (1994).

102. H.F. Fisher. (L. Xue, P. Talalay, and A.S. Mildvan, Studies of the Mechanism of the 5-3-Ketosteroid Isomerase Reaction by Substrate, Solvent, and Combined Kinetic Deuterium Isotope Effects on Wild-Type and Mutant Enzymes, Biochemistry, 29:7491, 1990; L Xue, A. Kuliopulos, A.S. Mildvan, and P. Talalay, Catalytic Mechanism of an Active-Site Mutant (D38N) of 5-3-Ketosteroid Isomerase. Direct Spectroscopic Evidence for Dienol Intermediates, Biochemistry, 30:4991, 1991; and L.Xue, P. Talalay, and A.S. Mildvan, Studies of the Catalytic Mechanism of an Active-Site Mutant (Y14F) of 5-3-Ketosteroid Isomerase by Kinetic Deuterium Isotope Effects, Biochemistry, 30:10858, 1991) Critical Reviews in Chemtracts, 6, 69-75 (1996).

103. H.F. Fisher and S.K. Saha. The Interpretation of Transient-State Kinetic Isotope Effects. Biochemistry, 35, 83-88 (1996).

104. S.J. Maniscalco, S.K. Saha, P. Vicedomine, and H.F. Fisher. A Difference in the Sequence of Steps in the Reactions Catalyzed by Two Closely Homologous Forms of Glutamate Dehydrogenase. Biochemistry, 35, 89-94 (1996).

105. N. Singh, Z. Liu, and H.F. Fisher. The Existence of a Hexameric Intermediate with

Molten-Globule-Like Properties in the Thermal Denaturation of Bovine-Liver Glutamate Dehydrogenase. Biophysical Chemistry, 63, 27-36 (1996) .

106. S.K. Saha, S.J. Maniscalco, and H.F. Fisher. Mechanistic Interpretation of Tryptophan Fluorescence Quenching in the Time Courses of Glutamate Dehydrogenase Catalyzed Reaction. Biochemistry, 35, 16483-16488 (1996).

107. H.F. Fisher and J. Tally. Isoergonic Cooperativity in Glutamate Dehydrogenase

Complexes: A New Form of Allostery. Biochemistry, 36 #36, 10807-10810 (1997).

108. S.K. Saha, S.J. Maniscalco, and H.F. Fisher. The Use of Multiwavelength Kinetic Analysis Approach to Identify and Characterize Intermediate Complexes in the Reductive Amination Reaction Catalyzed by Bovine Liver Glutamate Dehydrogenase. Biochim. Biophys. Acta, 1382, 8-12 (1998).

109. H.F. Fisher and J. Tally. Isoergonic Cooperativity—A Novel Form of Allostery. Methods in Enzymology, 295, 331-349, (1998).

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110. L. Indyk and H.F. Fisher. Theoretical Aspects of Isothermal Titration Calorimetry. Methods in

Enzymology, 295, 350-364 (1998).

111. H.F. Fisher, S.K. Saha, and S.J. Maniscalco, Identification and Characterization of Kinetically Competent Carbinolamine and α-Iminoglutarate Complexes in the Glutamate Dehydrogenase-Catalyzed Oxidation of L-Glutamate Using a Multiwavelength Transient State Approach, Biochemistry, 37, 14585-14590 (1998).

112. H.F. Fisher, Transient State Kinetic Approaches to the Resolution of Enzyme Mechanisms, in Enzymatic Mechanisms, eds. P.A. Frey and D.B. Northup IOS Press 264-277 (1998).

113. S.K. Saha, and H.F. Fisher, The Location of Active Site Opening and Closing Events in the Prehydride Transfer Phase of the Oxidative Deamination Reaction Catalyzed by Bovine Liver Glutamate Dehydrogenase Using a Novel pH Jump Approach. BBA 1431, 261-265 (1999).

114. H.F. Fisher, Protein Ligand Interactions Molecular Basis, Encyclopedia of Life Sciences, (In Press, 2000).

115. H.F. Fisher, Glutamate Dehydrogenase as a Molecular Machine, Advances in Enzymology, (In Press, 2000).

116. H.F. Fisher, Transient State Enzyme Kinetics (Accounts of Chemical Research,

Invited Paper, In Press)

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CURRICULUM VITAE Mark Thomas Fisher HOME ADDRESS: WORK ADDRESS: 4300 West 71st Terrace University of Kansas Medical Center Prairie Village, KS 66208 Department of Biochemistry and Molecular Biology (913) 432-1668 3901 Rainbow Boulevard

Kansas City, KS 66160-7421 Tel: (913) 588-6940 Fax: (913) 588-7440

DATE AND PLACE OF BIRTH: June 21, 1954; Spokane, WA Research interests: Roles of molecular chaperones in protein-folding and

oligomer assembly, Thermodynamics and kinetics of protein folding, second derivative UV spectroscopy, electron transfer in proteins

EDUCATION: 1976 - B.S. Microbiology, Purdue University, Lafayette, IN

1982 - B.S. Chemistry, Purdue University, Hammond, IN 1987 - Ph.D.Biochemistry, University of Illinois, Urbana, IL

PROFESSIONAL EXPERIENCE:

2008-present Professor, Department of Biochemistry and Molecular

Biology, University of Kansas Medical Center, Kansas City, KS

1997- 2008 Associate Professor, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS

1995-Present Adjunct Member of University of Missouri Doctoral Faculty

1992 - 1997 Assistant Professor, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center,

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Kansas City, KS

1987 - 1992 Staff Fellow, Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD

Jan-May 1987 Research Associate, Department of Biochemistry, University of Illinois

1983 - 1987 Research Assistant, Department of Biochemistry, University of Illinois

1982 - 1983 Teaching Assistant, Department of Biochemistry, University of Illinois

1981 - 1982 Undergraduate Research Assistant, Department of Chemistry, Purdue University

1981 - 1982 Laboratory Technician III, Department of Biochemistry, Rush-St. Luke's Presbyterian Hospital, Chicago, IL

Grant Study Sections Member NIH Special Study Section SPECIAL EMPHASIS PANEL ZRG1 BST-L (50) Targeting Disease Caused by Protein Misfolding or Misprocessing February 13-14, 2006 Member NSF Study Section SBIR/STTR Bioprocess I. Division of Industrial Innovation and Partnerships (IIP) January 31, 2007 Member NIH Special Study Section SPECIAL EMPHASIS PANEL ZRG1 BST-Q(52) Targeting Disease Caused by Protein Misfolding or Misprocessing June 8, 2007. Member NSF Study Section SBIR/STTR Bioprocess, Phase II. Division of Industrial Innovation and Partnerships (IIP) November 1, 2007, October 21, 2008. PATENTS Fisher Mark .T. and Voziyan Paul A. “Chaperonin and osmolyte protein folding and related screening methods” Patent # 6,887,682 United States Patent and Trademark Office issued December 2004 – Published May 6 2005.

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Submitted a continuation in Part (CIP) (additional claims to commercialized array – will be issued as another patent) Mark T. Fisher and Paul A Voziyan “Chaperonin and osmolyte protein folding and related screening methods” Attorney Docket No. 506275-0025. In process. Mark T. Fisher and Hiroo Katayama UNITED STATES Pending PATENT : OSMOLYTE MIXTURE FOR PROTEIN STABILIZATION, Attorney Docket No.: 506275-0044

Mark T. Fisher and Subhashchandra Naik UNITED STATES Pending PATENT: SYSTEMS AND METHODS FOR IDENTIFYING PROTEIN STABILIZERS Attorney Docket No

Current Funding:

D. Research Support (Current) • NIH T32A1070089 NIH Training Grant- Biotechnology Grant, (Mentor -

Graduate student Training Fellowship)

• NIH R01- CryoEM analysis of Anthrax Toxin Pore Complexes , March 1, 2011- Feb 29th 2014. 775,000 direct and indirect

• Leid Grant – Detection/ Prevention of Preaggregate Formation in Concentrated Therapeutics February 15, 2013-Feb 14, 2014. 30 K Direct costs D. Volkin coPI.

• KUCTC Proof of Concept grant “Designing Broad-Based HTS Platforms to Detect and Stabilize Partially Folded/Misfolded Protein Products.” April 1, 2013-March 31, 2014. 50 K D. Volkin coPI.

Completed (last 4 years) Institute for Advancing Medical Innovation. - “Lead Compound Identification of Direct Chemical Correctors of the Misfolding of Cystic Fibrosis Transmembrane Regulator using a Novel Chaperonin Screening System” $100,000 K Total. June 1, 2010- December 31, 2011. Oncimmune Industrial Refolding Contract – Successful Cancer Detection Product Development. Refolded 11 Oncogenic proteins. Sept 2008-Sept 2010 MCB 0445936 Fisher (PI) 07/15/05-07/14/09 National Science Foundation (5 publications)

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“Structural and Energetic Effects of Protein Substrate Binding on GroEL Chaperonin” Kansas City Life Sciences initiative – Hookworm Antigen development and Production July 2009-June 2010. $45,000 Courses taught: Biochem 801 Medical Biochemistry discussion groups IGPBS 891 Module 1 Biochem 923 Protein Structure and function Biochem 802 – Formal Seminar Course Biochem 805 - independent study biochemistry course Biochem 808 – Physical Biochemistry… renamed Methods for Analyzing Biomolecules Biochem 862 – Literature series – all Biochemistry Graduate students IGPBS Introductory lecture series GSMC 859- Research Rotations (2-4 credit hours) GSMC 852 -Introduction to Biomedical Research (2 credit hours) GSMC 858 - Introduction to Faculty Research (1 credit hour) GSMC 850 - Proteins and Metabolism (2 credit hours) Biochem Foundations in Medicine, Genetics and Neoplasia KU Course Molecular Biosciences - Protein Structure function course - Chaperone Proteins KU Mini College Course (KU Alumni) - Protein stability and disease. Outside teaching lectures UMKC Premedical Scholars Lectureship – two lectures William Jewell College – Invited teaching lecture – Protein folding and disease. Missouri State – Protein Folding – Current state of the art Missouri Western- Bacterial Toxin Lecture. Students Trained Past Ph.D. Student Direct Advisor (supervised thesis work directly) Chee Ming Low – MS – 1999 – Current Senior Scientist position at Pzifer US. Bryan Tieman – MS – 2001 Current Senior Scientist at Abbott Laboratory Scott Falke – Ph.D.- 2004 –Current Assoc. Professor in Biology at William

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Jewell. Greg Bomhoff – MS- 2007- Current High School Teacher- St Joseph Hiroo Kayatama – 6 papers 3 first author – Dropped out of Ph.D. program- medical reasons Summer 2012. Subhashchandra Naik –Ph.D. - 3 first author papers – plus 2 in preparation. Defended Dec 2012- Current Postdoctoral Fellow in My laboratory C) Ph.D. Supervisory and Final Dissertation Examination Committee (Member or Chairperson* for the following Students)

Name Date Completed on Campus

Program

Stephen Clum Ph.D. Fall 1997 Biochemistry and Molecular Biology

Chee Ming Low* MS Fall 1997 Biochemistry and Molecular Biology

LiLin Wang Ph.D. Fall 1998 Microbiology and Immunology

Bryan Tieman* MS Fall 1999 Biochemistry and Molecular Biology

Shihyun You Ph.D. Fall 2000 Biochemistry and Molecular Biology

Stephen Parnell Ph.D. Spring 2001 Biochemistry and Molecular Biology

Brian Lobo Ph.D. Fall 2002 KU Pharmaceutical Chemistry Department

Scott Falke* Ph.D. Fall 2003 Biochemistry and Molecular Biology

Huimin Jiang Ph.D. Spring 2004 Biochemistry and Molecular Biology

Roberto Vanacore* Ph.D. Fall 2005 Biochemistry and Molecular Biology

Melissa Khor Ph.D. Spring 2005 KU Pharmaceutical Chemistry Department

Alex Dajkovic Ph.D. Fall 2005 Microbiology and Immunology

Greg Bomhoff MS. Spring 2006 Biochemistry and Molecular Biology

Tim Priddy Ph.D. Fall 2006 UMKC Biochemistry Department

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Hiroo Katayama left due to Medical reasons 2012

Biochemistry and Molecular Biology (6 papers)

Subhashchandra Naik Ph.D.

Spring 2013 Biochemistry and Molecular Biology

Maurico Uribe-Vargas 2008 - Present Biochemistry and Molecular Biology

Alex McDowell 2009- Present Microbiology and Immunology

Stephanie Bishop 2011- present Pharmacology and Toxicology

Comprehensive examinations (Oral and written) – predoctoral program.* chair Name Date of Comp. Department

Steven Parnell Spring 1998

Biochemistry and Molecular Biology

Shihyun You Spring 1998 Biochemistry and Molecular Biology

Susanna Harju* Spring 1999

Biochemistry and Molecular Biology

Scott Falke Spring 2000

Biochemistry and Molecular Biology

Daniel Kim Spring 2000

Biochemistry and Molecular Biology

Roberto Vanacore* Spring 2001

Biochemistry and Molecular Biology

Ryan Shultze Spring 2005

Microbiology and Immunology

Hiroo Katayama July 2007

Biochemistry and Molecular Biology

Shuari Lu Spring 2008

Biochemistry and Molecular Biology

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Subhashchandra Naik Spring 2009

Biochemistry and Molecular Biology

Mauricio Vauge Uribe Spring 2010

Biochemistry and Molecular Biology

Alex McDowell Fall 2010

Molecular Biology and Immunology

Susan Brock

Spring 2014 Molecular Biology and Immunology

Past Ph.D. Candidate – IGPBS Rotation Students 6 week rotations and lab instruction with Presentations (starting October 2009) Fall 1992 Stephen Clum presented research results at the Student research forum in spring 1993, Fall 1992 one student Xuesong Yuan, included in Journal of Biological Chemistry article; Fisher, M.T., and Yuan, X. (1994) The Rates of Commitment to Renaturation of Rhodanese and Glutamine Synthetase in the Presence of the GroE Chaperonins. The Journal of Biological Chemistry 269, 29598-29601. Hitao Li Fall 1993; Simon Low Fall 1994, Joined my laboratory 1 paper Steven Parnell - Spring 1996, Joined Jim Calvets Laboratory Byran Tieman Fall 1997 Joined my laboratory – 2 papers, Scott Falke Fall - 1998 Joined my laboratory 3 papers Spring 2002 Nick Mueller, Greg Bomhoff. Joined my laboratory 2 papers Spring 2005 Aron Gottshalk – Joined Jon Conaway Laboratory Spring 2006 Hiroo Katayama Joined my laboratory 6 papers Fall, 2006 Jill Morris, Joined Page Gieger laboratory1 paper Spring 2007 Paul Welch, switched to MD student Summer 2007 Subhashchandran Naik, -Joined as Graduate student 3 papers 1 in preparation Summer 2007- MD/Ph.D. Student Bliss Hartnett Publication in Biopolymers, 2010, Fall 2007 – Mitchell Mcgill, Joined Pharm/tox Publication in J. Functional

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Genomics 2009. Fall 2009 Maurico Vega-Uribe Joined Alexey Ladokhin Spring 2010 2010 Julie Mitchell Joined Micro/Immunology dept Publication in Protein Science 2013, Cassandra Field November 2011, Joined Alexey Ladokhin laboratory Susan Brock Spring 2012, Joined Micro/Immunology Publication in Biochemistry 2013 Jackie Thompson October – December 2012. Presented at National Meeting Midwest stress Response, Joined Gerald Carlson Laboratory. (Additional Note: Not only advising but instruct student to present research at the end of their rotations 20 min presentation). Undergraduate students Advised. (summer and/or Fall internships). Cory Mclain – KU Lawrence Pharmacy School - KBRIN Grant – testing anti amyloid compounds and examining GroEL effects on amyloid formation. (June 2004). Ara Azad-Khorassani – KU Edwards campus (January 2005- 2 years) – testing chaperonin partitioning assay. Stephen Glaser – University of Kansas - Osmolytes and protein folding Josh Hamilton – University of Kansas- Osmolytes and protein folding Kate Prather – William Jewell College- 2 years- completed single ring purification. Marek Brzozowski – University of Kansas - Single particle analysis – Network specialist Jordan Jurcyk – Washington USingle particle analysis Andrew Kerns – K- State- GST-PEPCK folding Derek Low – K- State- GST-PKD purification and folding project. Nick Degner- University of Kansas - Chaperonin/Osmolyte Screen. 2010-2011 Jack Walker – Washington U.- Nanodisc preparation and protein insertion 2 summers 2011- 2012 Elizabeth Lindboe- Reed College -GroEL detection of antibody Preaggregates 2 summers 2011- 2012 Medical Scholar Advisor 2 summer rotations. Kirk Sloan – Medical Scholars Grant – testing anti amyloid compounds (July 2004) 1 paper coauthorship published. Jacob Hodges, Medical Scholars Grant – Chaperonin sink with TTR (July 2006)- 1 paper coauthorship published. Outside Research Activities High School students scholars Research Advisor - Chung Lee (Shawnee-Mission South High School) 12/94-

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5/98 Two time Grand Award Winner (as a freshman and Senior) - Kansas City Science and Engineering Fair Third Place - National competition Projects: "The Probing of ATP-Dependent Conformational Changes in GroEL Using Proteases" “The role of ATP in the Chaperonin mechanism - A four year study” Research Advisor – Katie George- High School Teacher - JCCC Tech Prep Grant 2 weeks July –concentrating folded proteins from chaperonin osmolyte system. Research advisor – Richard Kaufman – High School teacher – Normal mode flexible fitting (See NSF RET funding). Research Advisor Current – Roshan Bisarya - Olathe North High School – Osmolyte mixture studies with GFP rescue with GroEL. Past Postdoctoral Candidates trained Kirk Smith – Current Assistant Professor Paul Voziyan – Current Research Associate Professor at Vanderbilt- 6 papers. Inam Haque – Current research Assistant professor at VA Hospital Kansas City KS 1 paper Boris Kornilayev – Current Research Associate at UMKC Biochemistry 1 paper. Narahari Akkaladevi – July 2011- Sept 2013 - 3 papers published (2 in preparation) Srayanta Mukherjee - Dec. 2011 May 2013– 2 papers published 2 separate papers in preparation. Current Postdoctoral fellows: Wendy Lea – April 2013-Current Divya Amin – September 2013 Subhashchandra Naik- March 2013 PUBLICATIONS: Naik, S., Brock S., Akkaladevi N., Tally J., Gao P., Zhang N., Pentelute, B. L.,

Collier R. J., Fisher, M. T. (2013) “Monitoring the kinetics of the pH driven transition of the anthrax toxin prepore to the pore by biolayer inferferometry and surface plasmon resonance ” Biochemistry Sep 17;52(37):6335-47. (Biochemistry Journal Website Highlights)

Gogol EP, Akkaladevi N, Szerszen L, Mukherjee S, Chollet-Hinton L, Katayama H, Pentelute BL, Collier RJ, Fisher MT.(2013) “Three dimensional structure

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of the anthrax toxin translocon-lethal factor complex by cryo-electron microscopy.” Protein Sci. 2013 22:586-94 (Cover & Highlights)

Akkaladevi N, Hinton-Chollet L, Katayama H, Mitchell J, Szerszen L, Mukherjee S, Gogol EP, Pentelute BL, Collier RJ, Fisher MT. (2013) “Assembly of anthrax toxin pore: lethal-factor complexes into lipid nanodiscs.” Protein Sci. 22:492-501. (in new Methods and Applications paper)

Naik S., Zhang N, Phillip Gao P., and Fisher M.T. (2012) “On the Design of Broad Based Screening Assays to Identify Potential Pharmacological Chaperones of Protein Misfolding Diseases.” Current Opinions in Medicinal Chemistry vol 12:2504-22.

Henriques B.J., Fisher M.T., Bross P., Gomes C.M. (2011) “A polymorphic position in electron transfer flavoprotein modulates kinetic stability as evidenced by thermal stress.” FEBS Lett. 585(3):505-10.

Katayama H., Wang J., Tama F., Chollet L., Gogol E.P., Collier R.J., and Fisher M.T. (2010) “Three-Dimensional Structure of the Anthrax Toxin Pore Inserted into Lipid Nanodiscs and Lipid Vesicles” Proc. Natl. Acad. Sci. USA. 107, 3453-3457.

Naik S, Haque I, Degner N, Kornilayev B, Bomhoff G, Hodges J, Khorassani AA, Katayama H, Morris J, Kelly J, Seed J, Fisher MT. (2010) Identifying protein stabilizing ligands using GroEL. Biopolymers. 93, 237-51.

Katayama H., McGill M., Kearns A., Brzozowski M., Degner N., Harnett B., Kornilayev B., Matkovic-Calogovic D., Holyoak T., Calvet J.P., Gogol E.P., Seed J., and Fisher MT., (2009) “Strategies for folding of affinity tagged proteins using GroEL and osmolytes” J. Structural and Functional Genomics. 10, 57-66. epub Dec 2008.

Katayama, H. Janowiak, B.E., Brzozowski, M., Juryck, J., Falke, S., Gogol E.P., Collier, R.J., and Fisher, M.T. (2008) “GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore” Nat. Struc. and Mol. Biol. 15, 754-60. (Featured in Highlights).

Fisher, M.T. (2006) “Proline to the Rescue” Proc. Natl. Acad. Sci. 103, 13265-13266. Bomhoff G., Sloan K., McLain C., Gogol E.P., and Fisher M.T. (2006) "The

effects of the flavonoid Baicalein and osmolytes on the Mg 2+ Accelerated Aggregation/Fibrillation of Carboxymethylated Bovine 1SS-α-Lactalbumin" Archives of Biochemistry and Biophysics 453 75-86.

Fisher, M.T. (2006) "Molecular Roles of Chaperones in Assisted Folding and Assembly of Proteins" Genetic Engineering, Principles and Methods 27, 183-220.

Braun CS, Fisher MT, Tomalia DA, Koe GS, Koe JG, Middaugh CR (2005) “A stopped-flow kinetic study of the assembly of nonviral gene delivery complexes.” Biophys J. 88, 4146-58.

Falke. S.R., Tama F. Gogol. E.G. and Fisher M.T. (2005) “Structural Changes in

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GroEL Induced by Protein Substrate Binding J. Mol. Biol.. 348, 219-230.

Voziyan P.A , Johnston M., Chao A., Bomhoff G. and Fisher, M.T. (2005) “Designing a high throughput refolding array using a combination of the GroEL chaperonin and osmolytes. J. of Structural and Functional Genomics 6, 183-188 .

George, R., Kelly S.M., Price, N.C., Erbse A., Fisher, M.T. and Lund. P.A. (2004) “Three GroEL homologues from Rhizobium leguminosarum have distinct in vitro properties. Biochem. Biophys. Res. Comm. 324, 822-828.

Hui Koon Khor, H.K., Fisher, M.T., and Schoneich C. (2004) “Potential role of methionine sulfoxide in the inactivation of the chaperone GroEL by hypochlorous acid (HOCl) and peroxynitrite (ONOO-)” J. Biol. Chem., 279, 19486-19493.

Voziyan Paul and Fisher M.T. (2002) Polyols induce folding of GroEL-bound bacterial glutamine synthetase without ATP. Archives of Biochemistry and Biophysics 397, 293-297.

Tieman, B.C., Johnston M. and Fisher, M.T. (2001) “Differences in Chaperonin Assisted Renaturation of Structurally and Sequentially Homologous Forms of Malate Dehydrogenase”. J. Biol. Chem. 276 44541-44550.

Liegh-Edwards K, Kueltzo, L.A., Fisher M.T. and Middaugh C.R. (2001) “Complex Effects of Molecular Chaperones on the Aggregation and Refolding of Fibroblast Growth Factor-1 ” Archives of Biochemistry and Biophysics 393, 14-21.

Falke S.F., Fisher M.T. and Gogol E.G. (2001) “Classification and reconstruction of a heterogeneous set of electron microscopic images: A case study of GroEL-substrate complexes” J. Struct. Biol.133, 203-213.

Falke S.F., Fisher M.T. and Gogol E.G. (2001) “Analysis of GroEL- Glutamine synthetase complexes by Electron Microscopy” J. Mol. Biol.(cover) 308, 569-577

Voziyan P.A. and Fisher M.T. (2000) “GroE-mediated folding of glutamine synthetase under non-permissive conditions; off-pathway aggregation propensity does not determine the co-chaperonin requirement” Protein Science 9, 2405-2415.

Voziyan P.A. Jadhav, L and Fisher M.T. (2000) “ Refolding a glutamine synthetase truncation mutant in vitro: identifying superior conditions using a combination of chaperonins and osmolytes.” The Journal of Pharmaceutical Sciences 89, 1036-1045.

Smith K.E., Voziyan P.A., and Fisher M.T. (1998) “Partitioning of rhodanese onto GroEL: Chaperonin binds a reversibly oxidized form derived from the native protein” The Journal of Biological Chemistry 273 28677-28681.

Voziyan P., Tieman B. Low C. M, and Fisher, M.T. (1998) “Initial interactions between GroE chaperonin complexes with Protein folding intermediates: Comparison of Different Substrates The Journal of Biological Chemistry

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273, 25073-25078.

Fisher, M.T. (1998) “GroE chaperonin-assisted folding and assembly of dodecameric glutamine synthetase Biochemistry (Moscow) Review 63, n4 453-472.

E. Eisenstein, P. Reddy and M. T. Fisher Overexpression, Purification and Properties of GroES from Escherichia coli in Methods. Enz. Protein Folding: Catalysts, Accessory Proteins and Chaperones eds George E. Lorimer and Thomas O. Baldwin (1997) Volume 290 p119- 135.

Smith, K.E. and Fisher, M.T. (1995): Interactions between the groE chaperonins and Rhodanese: Multiple intermediates and Release and Rebinding. The Journal of Biological Chemistry 270, 21517-21523.

Fisher, M.T., and Yuan, X. (1994) The Rates of Commitment to Renaturation of Rhodanese and Glutamine Synthetase in the Presence of the GroE Chaperonins. The Journal of Biological Chemistry 269, 29598-29601.

Phadtare, S., Fisher, M.T., and Yarbrough, L.R. (1994) Refolding and Release of Tubulins by a Functional Immobilized groEL Column. Biochem. Biophys. Acta. 1208, 189-192.

Fisher, M.T., (1994) The Effect of GroES on the GroEL-Dependent Assembly of Dodecameric Glutamine Synthetase in the Presence of ATP and ADP. The Journal of Biological Chemistry 269, 13629-13636.

Fisher, M.T. (1993): On the assembly of dodecameric glutamine synthetase from stable chaperonin complexes. The Journal of Biological Chemistry 268, 13777-13779.

Fisher, M.T. (1992): Promotion of the in vitro renaturation of dodecameric glutamine synthetase from Escherichia coli in the presence of GroEL (Chaperonin-60) and ATP. Biochemistry 31, 3955-3963.

Fisher, M.T. and Stadtman, E.R. (1992): Oxidative modification of E. coli glutamine synthetase: Decreases in the thermodynamic stability of protein structure and specific changes in the active site conformation. The Journal of Biological Chemistry 267, 1872-1880.

Fisher, M.T. (1991): Differences in thermal stability between reduced and oxidized cytochrome b562 from Escherichia coli. Biochemistry 30, 10012-10018.

Han, M.K., Cyran, F.P., Fisher, M.T., Kim, S.H. and Ginsburg, A (1990): Xenopus transcription factor IIIA: Evidence for heterogeneity of Zn2+ binding affinities and specific labeling of cysteine 287. The Journal of Biological Chemistry 265, 13792-13799.

Shrake, A., Fisher, M.T., McFarland, P.J. and Ginsburg, A (1989): Partial unfolding of dodecameric glutamine synthetase from Escherichia coli: Temperature-induced, reversible transitions of two domains. Biochemistry 28, 6281-6294.

Stayton, P.S., Fisher, M.T. and Sligar, S.G. (1988): Determination of

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cytochrome b5 association reactions: Characterization of metmyoglobin and cytochrome P-450cam binding to genetically engineered cytochrome b5. The Journal of Biological Chemistry 263, 13544-13548.

Fisher, M.T. and Sligar, S.G. (1987): Temperature jump relaxation kinetics to the P-450cam spin equilibrium. Biochemistry 26, 4797-4803.

Fisher, M.T., White, R.E. and Sligar, S.G. (1986): Pressure dissociation of a protein-protein electron transfer complex. Journal of the American Chemical Society 108, 6835-6837.

Fisher, M.T. and Sligar, S.G (1985): Control of heme protein redox potential and reduction rate: Linear free energy relation between potential and ferric spin state equilibrium. Journal of the American Chemical Society 107, 5018-5019.

Fisher, M.T. and Sligar, S.G. (1985): Tyrosine motions in relation to the ferric spin equilibrium of cytochrome P-450cam. Biochemistry 24, 6696-6701.

Fisher, M.T., Scarlata, S.F. and Sligar, S.G (1985): High pressure investigations of cytochrome P-450 spin and substrate binding equilibria. Archives of Biochemistry and Biophysics 240, 456-466.

Book Chapters (not peer reviewed) Sligar, S., Fisher, M.T., and Carraway, K.(1986): Structure, function, and

fluctuations during P-450 electron transfer. In Cytochrome P-450: Biochemistry, Biophysics and Induction (Verezkey, L. and Magyar, K., eds.), Elsevier Developments in Biochemistry, Vol. 27, p. 49.

Murray, R., Fisher, M.T., Debrunner, P. and Sligar, S.(1985): Structure and chemistry of cytochrome P-450. In Metalloproteins (Harrison, P., ed.), Vol. I, Macmillan, London, pp. 157-206, 1985.

Fisher, M.T., Scarlata, S. and Sligar, S.(1985): Conformational changes of cytochrome P-450cam in relation to substrate binding and ferrin spin equilibria. In Microsomes and Drug Oxidations (Boobis, A., et al., eds.), Taylor and Francis, London, pp. 23-32, 1985.

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UNIVERSITY OF KANSAS SCHOOL OF MEDICINE Faculty Curriculum Vitae

I. PERSONAL DATA

First Name Joseph

Last name Fontes

Current Academic Rank Associate Professor

Department(s) Biochemistry and Molecular Biology

Office Address KLSIC 1075

Phone 913-588-9848

Fax 913-588-9896

Email jfontes@kumc.edu

PROFESSIONAL DEVELOPMENT

Undergraduate and Graduate Education

Years (Inclusive) Degree Institution

1983-1987 B.S. Pharmacology University of California, Santa Barbara

1987-1992 (December) Ph.D. Pharmacology and Toxicology

University of California, Davis

Postgraduate Education

Years (Inclusive) Degree Institution

January 1993 – July 1998 Post-doctoral Fellow University of California San Francisco/HHMI

Academic and Professional Appointments and Activities (List in chronological order. Please explain any discontinuity in professional experience)

Month and Year Position Institution

August 1998 – July 2004 Assistant Professor Cleveland State University

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August 2004 – December 2006

Associate Professor Cleveland State University

August 1998 – December 2006

Adjunct Investigator Lerner Research Institute of the Cleveland Clinic Foundation

January 2007 – Present Associate Professor University of Kansas Medical Center

July 2009 – Present Associate Professor, with Tenure

University of Kansas Medical Center

Professional Registration/Licensure

Year Number State

N/A

Professional Certification(s)

Date Board

N/A

Professional Societies and Affiliations

Date Organization (including offices held)

1987-Present American Association for the Advancement of Science

1991-Present American Society of Biochemistry and Molecular Biology

Honors and Awards (honorary societies, research awards, teaching and other awards)

Year Award

2009 Student Voice Award, Best Lecturer, Year 1, KUMC

2011 Student Voice Award, Best Lecturer, Year 1, KUMC

2012 Ruth Bohan Teaching Professorship

II. TEACHING ACTIVITIES (Evaluations and other evidence of quality teaching must be attached; this represents the

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teacher’s portfolio and should be accurately summarize ALL of your teaching activities.) See appendix 1 Brief statement of areas of teaching interest: Though I found the mechanics of teaching at a medical school very different than my previous experience at a comprehensive university, I have maintained my general teaching philosophy. Simply stated, I believe my job is to help students see the world in a new way. That is, my goal is for a student to encounter the world as a biologist. When approaching a problem, he or she should think like a scientist, applying the rules of scientific inquiry and his/her fund of knowledge to address that problem. If a student leaves a course (or series of classes) I have taught with a collection of facts but without a new way of seeing the world, I have not succeeded. My philosophy can be illustrated by describing my approach to lecturing in Foundations of Medicine. My teaching responsibility in Foundations is the “central dogma” of biology, i.e. DNA structure, repair, replication, transcription and protein translation. The over-arching theme of these lectures, which I state explicitly in my very first slide, is that life is “information made animate”. This is simply another way of saying that genotype is the basis of phenotype, certainly not a revolutionary idea. But getting students to view human beings as three-dimensional representation of a digital genetic code is challenging. I strictly adhere to this construct, returning to it repeatedly as the three-letter acronyms of molecular biology spool out during my lectures. I want students to view the molecular machinery that goes into maintaining and expressing DNA as serving the one purpose of creating a proxy (living being) to test the fitness of information in a given environment. If the information makes a successful being, it will be passed on. I firmly believe that this goes to the core of being a physician, that if one is to repair a human being, one needs to truly understand its biological reason for their existence. Of course this also applies to the microorganisms with which we interact. Many years after a student forgets what a given protein complex does, I hope they will retain the idea that the expression and maintenance of DNA is, on the biological level, the meaning of life. 1. Instruction:

Didactic (e.g.: lectures and formal presentations at KUMC)

Academic Year Course Title

Instruction Student

Type Hours No Type

2008-2009 Core 800 Foundations of Medicine

Lecture 10 175 M1

2008-2009 IGPBS 893

Module 3, Molecular Biology

Lecture 6 25 Graduate

2008-2009 BCHM 892

Adv Molecular Genetics

Lecture 8 5 Graduate

2009-2010 Core 800 Foundations of Medicine

Lecture 10 175 M1

2009-2010 IGPBS Module 2, Molecular

Lecture 6 24 Graduate

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Biology

2010-2011 Core 800 Foundations of Medicine

Lecture 10 175 M1

2010-2011 BCHM 892

Adv Molecular Genetics

Lecture 6 5 Graduate

2011-2012 Core 800 Foundations of Medicine

Lecture 10 191 M1

2012-2013 BCHM 892

Adv Molecular Genetics

Lecture 6 5 Graduate

2012-2013 Core 800 Foundations of Medicine

Lecture 15 211 M1

Nondidactic (e.g.: workshops, labs, and discussion groups)

Academic Year Course Title

Instruction Student

Type Hours No Type

1998-2006

(6 semesters)

Bio 688/888

Seminar in Molecular Biology

Discussion group

15 3-16 Graduate

2000-2001

(2 semesters)

Bio 435 Molecular Biology Techniques Laboratory

Laboratory 90 15 Undergrad

2007-2008 Core 800 Foundations of Medicine

Small Group

24 22 M1

2007-2008 Core 805 Genetics and Neoplasia

Small Group

12 22 M1

2008-2009 IGPBS 893

Module 3, Molecular Biology

Discussion group

4 22 Graduate

2008-2009 Core 800 Foundations of Medicine

Small Group

12 20 M1

2008-2009 IGPBS 893

Module 3, Molecular Biology

Discussion group

4 25 Graduate

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2009-2010 Core 800 Foundations

of Medicine Small Group

12 24 M1

2009-2010 Core 805 Genetics and Neoplasia

Small Group

12 24 M1

2010-2011 Core 800 Foundations of Medicine

Small Group

12 20 M1

2010-2011 Core 805 Genetics and Neoplasia

Small Group

12 20 M1

2011-2012 Core 800 Foundations of Medicine

Small Group

12 20 M1

2012-2013 Core 800 Foundations of Medicine

Small Group

12 20 M1

2012-2013 Core 805 Genetics and Neoplasia

Small Group

6 20 M1

D.

Master’s Theses and PhD Dissertations directed

Year Completed Student Name Thesis Title

Degree (Completed/In

process)

2003 Rajini Mudhasani

Regulation of MHC Class II Gene Transcription by BRG-1

Ph.D., completed (Cleveland State

Univ.)

2006 Wafa Al-Kandari

Regulation of MHC class II Gene Transcription by ZXDC

Ph.D. completed (Cleveland State

Univ.)

2006 Srikarthika

Jambunathan

Post-translational modification of the MHC II gene regulator, ZXDC

Ph.D. completed (Cleveland State

Univ.)

2005 Vandana Navalgund

The Role of ZXDA in MHC Class II Gene Transcription

M.S. completed (Cleveland State

Univ.)

2009 Rupa Koneni

Regulation of hematopoietic transcription factors Ph.D. completed

143

by ZXDC (Cleveland State

Univ.)

Supervision of Postdoctoral Fellows

Year Fellow Name Area of Study

2005-2006 Margot Freer, Ph.D. Regulation of MHC class II gene transcription

2008-2009 Soo-Dong Park, Ph.D. ZXDC interacting factors and function

2009- Jon Ramsey, Ph.D. ZXDC in myelopoiesis

Advising (Thesis or dissertation committees: student academic group/individual) only KUMC listed

Date Student or group

name Type of Student/group

2008-2011 Won-Mee Park Individual, dissertation committee

2009-2012 Anastasiia

Aleksandrova Individual, dissertation committee

2009- Lu Chen Individual, dissertation committee

2009-2011 Aaron Chen Individual, dissertation committee

2009-2011 Kyung-Tae Park Individual, dissertation committee

2009-2010 Yun Chen Individual, dissertation committee

2010-2012 Yi Feng Individual, dissertation committee

2010- Xing Ma Individual, dissertation committee

2012- Allen Chazelle Individual, dissertation committee

2012- Zhen Zhang Individual, dissertation committee

2012- Nairita Roy Individual, dissertation committee

2012- Wahid Mulla Individual, dissertation committee

2012- Min Yao Individual, dissertation committee

Other teaching activities

144

Date Teaching Function Student

8/21/2009 PhD Comprehensive Exam Lu Chen

4/8/2009 PhD Comprehensive Exam Yun Chen

10/06/2009

and 4/21/2010 PhD Comprehensive Exam Won Mee Park

11/09/2009 PhD Comprehensive Exam Kyung-Tae Park

12/17/2009 PhD Comprehensive Exam Anastasiia Aleksandrova

11/15/2010 PhD Comprehensive Exam Yi Feng

06/15/2011 PhD Comprehensive Exam Xing Ma

08/31/2012 PhD Comprehensive Exam Rachel Grau

2. Development of Educational Materials (Course materials e.g. syllabi, educational software packages, web sites, films, educational tapes and evaluation tools)

Year Title Description Intended Audience

2012 Case of the Week Conferences M1 Medical Students

3. Educational Leadership (Responsibility for courses and other leadership activities including mentoring of junior faculty. Please list faculty members mentored – letters from mentorees may be provided.) Director, CORE 800, Foundations of Medicine. 2008-2008

Member and Vice Chair, Phase I Committee

Faculty Mentor for Liskin Swint-Kruse, Ph.D., Department of Biochemistry and Molecular Biology

SERVICE ACTIVITIES See guidelines and instructions to applicants for definitions and suggested documentation of professional and academic service. Professional Service: The diverse area of professional service includes patient care. Applicants should select measures that most clearly and concisely document their accomplishments and the value of these activities to the Medical School and University. Measures of both quantity and quality of activities are required and if necessary, applicants should provide brief descriptions to assist reviewers. Measures of patient care activities include numbers of patients, time allocation in clinical activity, procedures completed, Relative Value Units (RVUs), and value to the School of

145

the clinical service. If the primary quality evaluation is the subjective assessment of peers, this should be available in letters from departmental colleagues, chair, or referees. The significance of professional service in the forms of task forces, committees and similar groups should be explained and the specific role of the applicant clarified. Professional consulting services must have academic credibility and clear service intent and not be performed primarily for personal profit. Grant Review Panels:

1. Chair, NIH Special Emphasis Panel (2013/01 ZRG1 GGG-E (90) A Gene Expression and Regulation Area October 2012

2. Regular Member, NIH study Section Molecular Genetics B (MGB), 2007-2011 3. Ad Hoc Member, NIH Special Emphasis Panel, Academic Research Enhancement

Awards (R15), October 2007 4. American Heart Association, Ohio Valley Affiliate, 2005-2007 5. Environmental Protection Agency, Molecular Biology and Biochemistry, March 2007

Ad hoc manuscript reviewer: 1. Molecular and Cellular Biology 2. Molecular and Cellular Biochemistry 3. Journal of Molecular Biology 4. Journal of Biological Chemistry 5. Biochemical and Biophysical Research Communications 6. BBA-Gene Regulatory Mechanisms 7. Oncogene

Ad hoc external tenure/promotion review 1. Virginia Commonwealth University, 2012 2. University of Wisconsin Parkside, 2012

August 2012, Johns Hopkins Press, reviewer biochemistry text book proposal

Academic Service: In academic service the contribution of the candidate to the academic community should be clearly documented. Names and dates of committees, task forces, or working groups should be provided. A concise description of the significance of the group and explanation of the role of the applicant should be provided. Activities related to Academic Societies should be documented in this section.

Active: 1. July 2008-Present, Member, Phase I Committee 2. July 2011-Present, Vice-Chair, Phase I Committee 3. Oct 2012-Present, Member, Phase I Curriculum Content Working Group 4. August 2012-Present, Member, LCME Self Study Committee, Student Affairs 5. August 2012-Present, Member, LCME Medical Students Self Study Committee 6. September 2012- Present, Member, Academic and Professionalism Committee 7. September 2012-Present, Member Admissions and MD Student Selection

Committees 8. May 2012-Present, Faculty Advisor, Phi Delta Epsilon Medical Fraternity 9. 2011-Present, Elected At-Large Member, Faculty Assembly 10. 2011-Present, Elected At-Large Member, Faculty Assembly Executive Committee 11. 2010-Present, Faculty Advisor, Delp Society and Career Advising Program

146

Past: 1. January-April 2012, Developed graduation competencies for KU SOM (with Dr.

Anne Walling) 2. August-October 2011, Chair, Renal-Endocrine Module Review Committee 3. 2010-2011, Interviewer, medical student admissions

REARCH and SCHOLARLY ACTIVITIES: Brief statement of areas of research and scholarly interest, including current projects: My research interests lie in the regulation of gene transcription, primarily of major histocompatibility complex class II (MHC II) genes. The long term chromatin modifying complexes involved in activating MHC II gene transcription.

Three years ago we identified a family of novel transcription factors (ZXDA, ZXDB and ZXDC) involved in the regulation of MHC II genes. Subsequent and current work has focused on (1) the mechanism by which the ZXD family regulates gene transcription (2) additional target genes regulated by the ZXD family and (3) the role of ZXDC in development, particularly hematopoiesis. We now have data indicating that ZXD family members are involved in macrophage lineage determination, playing an important role in the development of the immune system. The mechanisms by which the ZXD family regulates macrophage development, including any potential role in leukemogenesis will be the focus of my lab in the future. We will also continue to use the ZXD family to study basic aspects of transcriptional regulation of genes.

1. Grants and contracts (Information must include whether the nominee is the principal investigator or a co-investigator, names of all investigators, title of grant, funding source, dollar amount in direct costs, and years during which grant applies. Co-investigators must specify role). Provide four copies of abstracts and award notice or renewal for all grants or contracts awarded in last five years (See Appendix II) Grants and contracts awarded: (List in chronological order)

Principal Investigator Title of Grant

Funding Source

Direct Costs Years Status

Fontes

Chromatin Remodeling and

MHC class II gene transcription

NIH (NIAID) $50,000/yr

9/2002-8/2004 Complete

Fontes

Regulators and regulation of MHC

class II gene transcription

NIH (NIAID) $175,000/yr

4/2005-12/2009 Complete

Fontes The Role of ZXDC

in Myelopoiesis NIH (NIAID) $50,000/yr 3/2011-

2/2012 Active

Belousov

Fontes Co-investigator

(10%)

NOVEL MECHANISM FOR

GLUTAMATE-DEPENDENT

EXCITOTOXICITY

NIH (NINDS)

$226,500

7/2012-6/2014 Active

Grants and contracts submitted:

147

Principal

Investigator Investigators Title of Grant Funding Source

Direct Costs Years Status

Fontes

Mechanisms of inflammatory

gene regulation by the

transcription factor ZXDC

NIH (R01) $250,000/yr 5

Submitted Oct

2012/Status pending

Fontes

The Role of Zinc Finger X-

Linked Duplicated

(ZXD) Family Members in

Myelopoiesis NIH

(R01) $250,000/yr 5

Not funded

Fontes

Non-coding RNAs, CTCF

occupancy and transcriptional

start site selection

NIH (R21) $175,000/yr 2

Not funded

Fontes

The Role of the ZXD

Transcription Factor Family in

Myelopoiesis KUMC (Lied) $35,000 1 Not funded

Fontes

Expression of the transcription factor ZXDC in Acute Myeloid

Leukemia

KUMC (Cancer Center) $35,000 1 Not funded

Fontes

L. Stehno-Bittel,

Andrei Belousov

Mechanisms of Connexin-36

gene expression and

the pathophysiology

of diabetes NIH

(R21) $175,000/yr 2

Not funded/ Resubmission

pending

Fontes

Inflammatory gene regulation

by the transcription factor ZXDC

AHA Grant-in-Aid $75,000/yr 2

Scored, not funded

Fontes

Regulation of Inflammatory

Gene Transcription by

ZXDC NIH

(R01) $250,000/yr 5 Pending

148

2. Scholarly Publications

Full length, peer-reviewed articles in scientific journals: (Provide names of all authors, year, title, journal, volume, and inclusive pages. List in chronological order.) Articles published: (Provide 4 copies of each peer-reviewed article published within the last five years. Other articles may be provided at the applicant's discretion.)

1. Fontes JD, Strawhecker JM, Bills ND, Lewis RE, Hinrichs SH.

Phorbol esters modulate the phosphorylation of human T-cell leukemia virus type I Tax.

J Virol. 1993 Jul;67(7):4436-41.

2. Chang CH, Fontes JD, Peterlin M, Flavell RA.

Class II transactivator (CIITA) is sufficient for the inducible expression of major histocompatibility complex class II genes.

J Exp Med. 1994 Oct 1;180(4):1367-74.

3. Fontes JD, Jabrane-Ferrat N, Toth CR, Peterlin BM.

Binding and cooperative interactions between two B cell-specific transcriptional coactivators.

J Exp Med. 1996 Jun 1;183(6):2517-21.

4. Jabrane-Ferrat N, Fontes JD, Boss JM, Peterlin BM.

Complex architecture of major histocompatibility complex class II promoters: reiterated motifs and conserved protein-protein interactions.

Mol Cell Biol. 1996 Sep;16(9):4683-90.

5. Lim CS, Jabrane-Ferrat N, Fontes JD, Okamoto H, Garovoy MR, Peterlin BM, Hunt CA.

Sequence-independent inhibition of RNA transcription by DNA dumbbells and other decoys.

Nucleic Acids Res. 1997 Feb 1;25(3):575-81.

6. Fontes JD, Jiang B, Peterlin BM.

The class II trans-activator CIITA interacts with the TBP-associated factor TAFII32.

Nucleic Acids Res. 1997 Jun 15;25(12):2522-8.

7. Fontes JD, Jabrane-Ferrat N, Peterlin BM.

Assembly of functional regulatory complexes on MHC class II promoters in vivo.

J Mol Biol. 1997 Jul 18;270(3):336-45.

8. Fontes JD, Kanazawa S, Jean D, Peterlin BM.

149

Interactions between the class II transactivator and CREB binding protein increase transcription of major histocompatibility complex class II genes.

Mol Cell Biol. 1999 Jan;19(1):941-7.

9. Mudhasani R, Fontes JD.

Inhibition of class II trans-activator function by HIV-1 tat in mouse cells is independent of competition for binding to cyclin T1.

Mol Immunol. 2002 Jan;38(7):539-46.

10. Mudhasani R, Fontes JD.

The class II transactivator requires brahma-related gene 1 to activate transcription of major histocompatibility complex class II genes.

Mol Cell Biol. 2002 Jul;22(14):5019-26.

11. Mudhasani R, Fontes JD.

Multiple interactions between BRG1 and MHC class II promoter binding proteins.

Mol Immunol. 2005 Apr;42(6):673-82.

12. Al-Kandari W, Jambunathan S, Navalgund V, Koneni R, Freer M, Parimi N,

Mudhasani R, Fontes JD.

ZXDC, a novel zinc finger protein that binds CIITA and activates MHC gene transcription.

Mol Immunol. 2007 Jan;44(4):311-21.

13. Al-Kandari W, Koneni R, Navalgund V, Aleksandrova A, Jambunathan S, Fontes JD.

The zinc finger proteins ZXDA and ZXDC form a complex that binds CIITA and regulates MHC II gene transcription.

J Mol Biol. 2007 Jun 22;369(5):1175-87.

14. Jambunathan S, Fontes JD.

Sumoylation of the zinc finger protein ZXDC enhances the function of its transcriptional activation domain.

Biol Chem. 2007 Sep;388(9):965-72.

15. Aleksandrova A, Galkin O, Koneni R, Fontes JD.

An N- and C-terminal truncated isoform of zinc finger X-linked duplicated C protein represses MHC class II transcription.

Mol Cell Biochem. 2010 Apr;337(1-2):1-7. 16. Lu J, Wang K, Rodova M, Esteves R, Berry D, Lezi E, Crafter A, Barrett M, Cardoso S, Onyango I, Parker W, Fontes J, Burns J, Swerdlow R, Polymorphic Variation in Cytochrome Oxidase Subunit Genes.

150

J Alzheimers Dis. 2010 Jan;21(1):141-54. 17. Wang Y, Denisova JV, Kang KS, Fontes JD, Zhu BT, Belousov AB. Neuronal gap junctions are required for NMDA receptor-mediated excitotoxicity: implications in ischemic stroke. J Neurophysiol. 2010 Dec;104(6):3551-6. 18. Park WM, Wang Y, Park SD, Denisova JV, Fontes JD, Belousov AB. Interplay of Chemical Neurotransmitters Regulates Developmental Increase in Electrical Synapses Journal of Neuroscience 2011 Apr 20;31(16):5909-20. 19. Wang Y, Song JH, Denisova JV, Park WM, Fontes JD, Belousov AB. Neuronal gap junction coupling is regulated by glutamate and plays critical role in cell death during neuronal injury. J Neurosci. 2012 Jan 11;32(2):713-25. 20. Song JH, Wang Y, Fontes JD, Belousov AB. Regulation of connexin 36 expression during development Neurosci Lett. 2012 Mar 28; 513(1):17-9 21. Belousov AB, Wang Y, Song JH, Denisova JV, Berman NE, Fontes JD. Neuronal gap junctions play a role in the secondary neuronal death following controlled cortical impact. Neurosci Lett. 2012 Aug 22;524(1):16-9. Manuscripts in press: (Provide names of all authors, title, journal, and 4 photocopies of manuscript plus evidence of acceptance by journal) N/A Manuscripts submitted but not yet accepted: (Provide names of all authors, title, journal, and 4 photocopies plus evidence of receipt of manuscript by journal.)

Invited or non-peer-reviewed articles or reviews: (Provide names of all authors, year, title, journal, volume, and pages. If in press, provide documentation and 4 photocopies if published within the last five years.)

1. Fontes, JD, Kanazawa S, Nekrep N, Peterlin BM. The class II transactivator CIITA is a transcriptional integrator. Microbes Infect. 1999 Sept; 1(11):836-9

2. Nekrep N, Fontes JD, Geyer M, Peterlin BM.

When the lymphocyte loses its clothes.

Immunity. 2003 Apr;18(4):453-7. Review.

3. Fontes, JD and Belousov, AB.

Neuronal gap junctions: making and breaking connections during development and injury

Trends in Neurosciences 2012 in press

Books and book chapters: (Provide names of all authors, year, book title, chapter title, edition, publisher, and pages. If in press, provide documentation and 4 photocopies if published the last five years.)

151

1. Hinrichs, S.H., L Chen, J.D. Fontes and G. Jay. Human T-Lymphotropic Virus Type I

(HTLV-I): Studies of disease mechanisms in a transgenic mouse. In: The Human Retroviruses. Jay, G. and Gallo, R., eds. Academic Press, Washington D.C., 1991

2. Hinrichs SH, J.D. Fontes, N.D. Bills, P.D. Schneider (1993) Transgenic models of human cancer. Princess Takamatsu Symposia, 1991, 22:259-74.

3. Presentations and posters Oral paper presentations: (Provide names of all authors, title, sponsoring organization, extent of peer-review, and location and date of presentation.) PLEASE NOTE: Most of my academic career was spent at Cleveland State University, which is an excellent institution but generally not well funded. As such, there were essentially no institutional funds for travel. My grant resources were so precious that I chose to use them entirely to support my lab, rather than on travel. As such, my participation at scientific meetings was nil. Invited seminars at other universities: (Provide title, sponsoring organization or institution, and date of presentation.)

February 2000 Cleveland Clinic Foundation, Dept of Cell Biology September 2002 University of Toledo, Dept of Biological Sciences November 2007 Dept of Immunology, Micro and Molec. Genetics, KUMC September 2008 Rockhurst University, Kansas City, MO November 2008 Stowers Institute for Medical Research, Kansas City, MO March 2009 Dept of Anatomy and Cell Biology, KUMC May 2009 University of North Dakota, Dept of Chemistry and Biochemistry, Fargo, ND April 2010 Case Western Reserve University, Dept of Genetics, Cleveland OH Nov 2010 Northwest Missouri State Univeristy, Dept of Chemistry, Maryville, MO March 2011 Southern Illinois University SOM, Department of Biochemistry, Carbondale, IL November 2011 Cleveland State University, Department of Biological Science, Cleveland OH

152

CURRICULUM VITAE

Jennifer Gerton

Associate Investigator

Stowers Institute for Medical Research

1000 E. 50th Street

Kansas City, MO 64110

jeg@stowers.org

Education

1997 Stanford University, Stanford, CA, Ph.D. Microbiology and Immunology

1990 Stanford University, Stanford, CA, B.A. Human Biology

Positions Held

1/14-present Investigator, Stowers Institute for Medical Research

1/09-12/13 Associate Investigator, Stowers Institute for Medical Research

7/09-present Associate Professor, Department of Molecular Biology and Biochemistry, University of Kansas Medical School

7/02-12/08 Assistant Investigator, Stowers Institute for Medical Research

3/03-6/09 Assistant Professor, Department of Molecular Biology and Biochemistry, University of Kansas Medical School

8/00-6/02 UCSF Postdoctoral Fellow, topic: chromosome dynamics in S. cerevisiae

Advisor: Joseph DeRisi, Ph.D.

6/97-7/00 UNC Postdoctoral Fellow, topic: DNA recombination and repair in S. cerevisiae

Advisor: Thomas D. Petes, Ph.D.

9/90-6/97 Graduate student, topic: HIV-1 integrase

Advisor: Patrick O. Brown, M.D., Ph.D.

Teaching Experience

2013 SIMR courses, Genomics Module, Transcription and Chromatin Module

2011-2012 SIMR course, Cell division and Chromosome Biology

2009-2012 KU Med IGPBS core curriculum

2004, 2005 Biochemistry 922, Molecular Genetics, KUMed 2003 QB3 Microarray Course Instructor

2001, 2002 Assistant Instructor, Cold Spring Harbor Course “Making and using DNA microarrays”

153

Research Support 2013 CdLS Foundation grant

2008-2013 NIH R01: Molecular Mechanisms of Chromosome Segregation in Yeast

2007-2009 Hudson Prize

2003-2005 Basil O’Connor March of Dimes Starter Scholar Research Award

2002 Herb Boyer Postdoctoral Fellowship 1999-2001 American Cancer Society Postdoctoral Fellowship

2000 Gordon Conference Travel Scholarship 1. Juan March Travel Scholarship 1997-1998 UNC Lineberger Cancer Center Postdoctoral Fellowship 1995 Helena Anna Henzl Gabor Travel Scholarship 1991-1995 NIH Training Grant recipient 1989-1990 Howard Hughes Undergraduate Researcher

Professional Associations and Service 2013-2018 Editorial Board, JBC

2012-present SIMR Travel Award committee

2011-present CdLS Foundation Research Committee, CdLS Clinical Advisory Board

2011-present MGA, GCAT NIH study sections, ad hoc

2009 Genetic Mechanisms of Inheritance NSF Panel

2005-present member, American Society for Cell Biology

2003-present member, Genetics Society of America

2007-2010 SIMR Microarray Advisory Committee

2004-2010 SIMR Supergroup

2007-2008 Advisory Board for Allen Press

2005-2008 SIMR IACUC

2002-2005 UMKC yeast/fungal group

Workshop Organizer

2013 Ribosome Function and Ribosomopathies, held at SIMR

2011 Ribosome Function and Ribosomopathies, held at SIMR

2004 The Structural Biology of Chromosomes, held at SIMR

Students advised

KU Med students-Ph.D. graduation

Ray Camahort 2008

154

Matthew Goering 2010

Shuai Lu 2013

Open University students-Ph.D. graduation

Manjunatha Shivaraju 2011

Karthik Dhatchinomoorthy expected 2016

Paris VI-Ph.D. graduation

Musinu Zakari expected 2014

Stowers graduate school-Ph.D. graduation

Kobe Yuen expected 2017

Dissertation committees

KU Med students-Ph.D.

Kristin Watt

Ashleigh Fritz

Lili Pan

Evan Janzen

Aaron Gottschalk

Rushi Trivedi

Open University students-Ph.D.

Guangbo Chen

Dominic Heinecke

Jin Zhu

Stowers graduate schoo-Ph.D.l

Soon-Keat Ooi

Graduate classes taught 2013 SIMR course, Genomics Module

2013 SIMR course, Transcription and Chromatin Module

2011-2012 SIMR course, Cell division and Chromosome Biology

2009-2012 KU Med IGPBS core curriculum

2004, 2005 KU Med Biochemistry 922, Molecular Genetics

Publications

155

Xu B., Lee K.K., Zhang L., Gerton J.L. 2013 Stimulation of mTORC1 with L-leucine rescues defects associated with Roberts syndrome, PLoS Genet, 9(10):e1003857

Gerton J.L. 2012 Translational mechanisms at work in the cohesinopathies, Nucleus, 3(6), 520-5.

Chen Z., McCroskey S., Guo W., Li H., and Gerton J.L. 2012 A genetic screen to discover pathways affecting cohesin function in S. pombe identifies chromatin effectors, G3, 2(10), 1161-8.

Shivaraju M., Unruh J.R., Slaughter B.D., Mattingly M., Berman J., Gerton J.L. 2012 Cell cycle coupled oscillation of centromeric nucleosomes in yeast, Cell, 150 (2), 304-316.

Preview in Cell, 150(2), 245-247.

See Research Highlights in Nature Reviews Genetics and Nature Reviews Molecular Cell Biology, Aug 2012

Bose T., Lee K.K., Lu S., Xu B., Harris B., Slaughter B.D., Unruh J.R., Garrett A., McDowell W., Box A., Li H., Peak A., Ramachandran S., Seidel C., Gerton J.L. 2012 Cohesin proteins promote ribosomal RNA production and protein translation in yeast and human cells, PLoS Genet, 8(6):e1002749

Shivaraju M. and Gerton J.L. 2011 The dynamics of the Cse4 chaperone Scm3, Cell Cycle 10(22):3823-4.

Shivaraju M., Camahort R., Mattingly M., and Gerton J.L. 2011 Scm3 is a centromeric nucleosome assembly factor, J Biol Chem, 286 (14):12016-23.

Reviewed in Faculty of 1000

Hewawasam G. and Gerton J.L. 2011. Cse4 gets a kiss-of-death from Psh1, Cell Cycle 10(4):566-7

Hewawasam G., Shivaraju, M., Mattingly M., Venkatesh S., Martin-Brown, S., Florens, L., Workman J.L. and Gerton J.L. 2010. Psh1 is an E3 ubiquitin ligase that targets the centromeric histone variant Cse4, Mol Cell, 40(3):444-454.

Preview in Mol Cell, 40(3):351-352

Reviewed in Faculty of 1000

Xiong B., Lu S., and Gerton, J.L. 2010 Hos1 is a lysine deacetylase for the Smc3 subunit of cohesin, Curr Biology, 20(18):1660-1665.

Lu, S., Goering, M., Gard S., Xiong B., McNairn, A.J., Jaspersen S., and Gerton J.L. 2010 Eco1 is important for DNA damage repair in S. cerevisiae, Cell Cycle 9(16): 3315-3327.

156

Bose T., and Gerton, J.L. 2010 Cohesinopathies, gene expression and chromatin organization, J. Cell Biol, 189(2): 201-210.

Xiong B., and Gerton, J.L. 2010 Regulators of the cohesin network, Annual Review Biochemistry, 79: 131-153.

Gard S., Light W., Xiong B., Bose T., McNairn, A.J., Harris, B., Fleharty B., Seidel C., Brickner J., and Gerton J.L. 2009. Cohesinopathy mutations disrupt the subnuclear organization of chromatin, J. Cell Biol, 187(4): 455-462.

Featured as Editor’s Choice, Science, 326(5959), Dec 11, 2009

Reviewed in Faculty of 1000

Camahort R., Shivaraju, M., Mattingly M., Li B., Florens L., Nakanishi S., Zhu D., Shilatifard A., Workman J. and Gerton J.L. 2009. Cse4 is part of an octameric nucleosome in budding yeast, Mol Cell 35: 794-805.

Reviewed in Faculty of 1000

McNairn, A.J., and Gerton J.L. 2009. Intersection of ChIP and FLIP: genomic methods to study the dynamics of the cohesin pathway, Chromosome Research, 17(2):155-63.

McNairn, A.J., and Gerton J.L. 2008. Cohesinopathies: one ring, many obligations, Mutation Research, 647(1-2):103-11.

McNairn, A.J., and Gerton J.L. 2008. The chromosome glue gets a little stickier, Trends in Genetics 24(8): 382-389.

Bausch C., Noone S., Henry J.M., Gaudenz K., Sanderson B., Seidel C., and Gerton J.L. 2007. Transcription alters chromosomal locations of cohesin in S. cerevisiae, Mol Cell Biol 27(24):8522-32.

Camahort, R., Li B., Florens L., Swanson S.K., Washburn M. and Gerton, J.L. 2007. Scm3 is essential to recruit the histone H3 variant Cse4 to centromeres and to maintain a functional kinetochore, Mol Cell 26: 1-13.

See Preview in Cell, 129, 1047-1049, 2007

See Research Highlights in Nature Reviews Molecular Cell Biology, Aug 2007

Reviewed in Faculty of 1000

Gerton, J.L. 2007. Enhancing togetherness:Kinetochores and cohesion, Gene Dev 21(3):238-41.

157

Collins K.A., Camahort R., Seidel C., Gerton J.L., and Biggins, S. 2007. The overexpression of a Saccharomyces cerevisiae centromeric histone H3 variant mutant protein leads to a defect in kinetochore biorientation, Genetics 175:513-525.

Henry, J.M., Camhort, R., Rice D. Florens, L., Washburn, M. and Gerton, J.L. 2006. Mnd1/Hop2 facilitates Dmc1-dependent interhomolog crossover formation in meiosis of budding yeast, Mol Cell Biol 26(8): 2913-2923.

Mieczkowski, P. A., Dominska, M., Buck, M. J., Gerton, J.L., Lieb, J. D., Petes, T. D. 2006. A global analysis of the relationship between the binding of the Bas1p transcription factor and meiotic recombination activity in Saccharomyces cerevisiae, Mol Cell Biol 26(3):1014-27.

Li, B. Pattenden, S.G., Lee, D., Gutierrez, J., Chen, J., Seidel, C., Gerton, J.L., and Workman, J.L. 2005. Preferential occupancy of histone variant H2AZ at inactive promoters influence local histone modifications and chromatin remodeling, Proc Natl Acad Sci 102(51):18385-90.

Gerton, J.L. and Hawley, R.S. 2005. Homologous chromosome interactions in meiosis: diversity amidst conservation, Nat Rev Genet, 6(6):477-87.

Gerton, J.L. 2005. Chromosome cohesion: a cycle of holding together and falling apart, PLoS Biol, 3(3):e94.

Glynn, E.F., Megee, P.C., Yu, H.G., Mistrot, C., Unal, E., Koshland, D.E., DeRisi, J.L., Gerton, J.L. 2004. Genome-wide mapping of the cohesin complex in the yeast Saccharomyces cerevisiae, PLoS Biol 2(9):e259.

see News and Views in Nature, 430: 520-521, 2004.

see comment in Journal of Cell Biology, July 2004.

Reviewed in Faculty of 1000

Weber, S.A., Gerton, J.L., Polancic, J.E., DeRisi, J.L., Koshland, D., and Megee, P.C. 2004. The kinetochore is an enhancer of pericentric cohesin binding, PLoS Biol 2(9):e260.

Gerton, J.L. and DeRisi, J. 2002. Mnd1p: a spliced evolutionarily conserved protein required for meiotic recombination, Proc Natl Acad Sci 99:6895-6900.

Kearney, H., Kirkpatrick, D., Gerton, J.L., and Petes, T.D. 2001. Meiotic recombination involving heterozygous large insertions in S. cerevisiae: Formation and repair of large, unpaired DNA loops, Genetics 158:1457-1476.

Reviewed in Faculty of 1000

158

Gerton, J.L., DeRisi, J., Shroff, R., Lichten, M., Brown, P.O., and Petes, T.D. 2000. Global mapping of meiotic recombination hotspots and coldspots in the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci 97:11383-11390.

Gerton, J.L., Herschlag, D., and Brown, P.O. 1999. Stereospecificity of reactions catalyzed by HIV-1 integrase. J Biol Chem 274:33480-33487.

Gerton, J.L., Ohgi, S., Olsen, M., DeRisi, J., and Brown, P.O. 1998. Effects of mutations in residues near the active site of HIV-1 integrase on specific enzyme-substrate interactions. J Virol 72:5046-5055.

Gerton, J.L. and Brown, P.O. 1997. The core domain of HIV-1 integrase recognizes key features of its DNA substrates. J Biol Chem 272:25809-25815.

Ellison, V., Gerton, J., Vincent, K. and Brown, P.O. 1995. An essential interaction between distinct domains of HIV-1 integrase mediates assembly of the active multimer. J Biol Chem 270: 3320-3326.

Harrington, J., Hsieh, C., Gerton, J., Bosma, G., and Lieber, M. 1992. Analysis of the defect in DNA end joining in the murine scid mutation. Mol Cell Biol 12: 4758-4768.

Seminar Presentations 2014 Queenstown Research Week, New Zealand

2014 FASEB-Yeast Chromosome structure, replication, and segregation

2014 CdLS National Scientific symposium

2013 Duke University, University Program in Genetics and Genomics

2013 Cohesin Biology and the Cohesinopathies, Certosa di Pontignano, Italy 2013 The Dynamic Nucleus of the Cell: Chromatin, Chromosomes, and Disease, Northwestern

2013 University of Texas, San Antonio, Department of Molecular Medicine

2013 University of Kansas, Edwards, Molecular Biology lab course

2012 University of Virginia, Department of Microbiology, Immunology, and Cancer Biology

2012 EMBO Centromere and Kinetochore workshop

2012 Sanjay Gupta’s visit to SIMR

2012 CdLS National Scientific symposium

2012 Cell Cycle, Cold Spring Harbor

2011 NIH, NCI

2011 Memorial Sloan Kettering Cancer Center

2011 Cohesin Biology and the Cohesinopathies, Il Ciocco, Italy

2010 Midwest Yeast Meeting, Northwestern University

159

2010 FASEB Yeast chromosome structure, replication, and segregation

2010 CdLS National Scientific symposium

2010 University of Virginia, Symposium on Mechanisms of genome stability

2009 University of Iowa, Department of Biology

2009 Cohesin Biology and the Cohesinopathies, Certosa di Pontignano, Italy 2009 Blaffer Seminar Series, MD Anderson Cancer Center 2008 Dynamic Organization of Nuclear Function, Cold Spring Harbor 2008 Department of Microbiology and Molecular Genetics, UMDNJ

2008 American Society for Biochemistry and Molecular Biology Annual Meeting

2007 American Society for Human Genetics, Cohesinopathies

2007 Transcription, Chromatin, and Cancer, Stowers Institute for Medical Research

2007 Midwest Yeast Meeting, Northwestern University

2007 AWIS meeting, William Jewell College

2006 Dartmouth College, Department of Biological Sciences

2006 FASEB Research Conference on Yeast Chromosome structure, replication, and segregation

2006 University of Rochester, Department of Biology

2006 KUMed, Workshop on Open Access publishing

2006 Bioinformatics 3640, Rockhurst University

2005 Cold Spring Harbor, Eukaryotic Mechanisms of Transcription

2005 International Conference of Yeast Genetics and Molecular Biology

2005 American Society for Microbiology Annual Meeting

2005 William Jewell College

2004 St. Louis University Cancer Center

2004 FASEB Research Conference on Yeast Chromosome structure, replication, and segregation

2004 Gordon Research Conference on Meiosis

2004 Stowers Institute, Structural Biology of Chromosomes Symposium

2004 University of Kentucky, Department of Molecular and Biomedical Pharmacology

2003 University of Kansas Medical School, Department of Biochemistry and Biophysics

2003 University of Missouri, Kansas City

2002 University of Kansas, Lawrence

2002 University of North Carolina, Department of Biology

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February 2, 2015

UNIVERSITY OF KANSAS SCHOOL OF MEDICINE Faculty Curriculum Vitae

III. PERSONAL DATA

First Name Liskin

Last name Swint-Kruse

Current Academic Rank Associate Professor

Department(s) Biochemistry and Molecular Biology

Office Address 1077 KLSIC

Phone 913-588-0399

Fax 913-588-9896

Email lswint-kruse@kumc.edu

PROFESSIONAL DEVELOPMENT

Undergraduate Education

Years (Inclusive) Degree Institution

1986-1990

1990-1995

B.S., Chemistry

Ph.D., Biochemistry

Baylor University, Waco TX

The University of Iowa,

Iowa City, IA

Postgraduate Education

Years (Inclusive) Degree Institution

1995-1999

Postdoctoral interdisciplinary studies in Computational Biology

W. M. Keck Center for Computational Biology, Rice University (Houston TX) and The University of Houston (Houston, TX)

161

2000-2002

Postdoctoral studies in Biochemistry

Rice University, Houston, TX

Academic and Professional Appointments and Activities (List in chronological order. Please explain any discontinuity in professional experience)

Month and Year Position Institution

July 2002 – May 2004

May 2004 – Sept 2004

Sept 2004 – Jun 2009

July 2009 - present

July 2009 - present

July 2009 – present

Oct 2011 - present

Research scientist

Moving to KC;

Advance work for KUMC

Assistant professor

Courtesy appointment

Associate Professor

Graduate Director

Associate Director, Interdisciplinary Graduate Program in Biomedical Sciences

Rice University, Houston, TX

The University of Kansas School

of Medicine, Department of

Biochemistry

The University of Kansas - Lawrence, Department of Molecular Biosciences

The University of Kansas School

of Medicine, Department of

Biochemistry

The University of Kansas School

162

of Medicine, Department of

Biochemistry

The University of Kansas School of Medicine

Professional Societies and Affiliations

Date Organization (including offices held)

1990 – present

2004 – present

1994 – present

2004 – present

2004 – present

American Chemical Society

American Association for the Advancement of Science

Gibbs Society in Biothermodynamics

Biophysical Society

Protein Society

Honors and Awards (honorary societies, research awards, teaching and other awards)

Year Award

1986-1990 Welch Scholar (for exceptional students majoring in Chemistry in the state of TX)

1986-1990 National Merit Scholar

1987 CRC Freshman Chemistry Award (Baylor University)

1987 National Mathematical Modeling Contest, Honorable Mention (Baylor University)

1988 Chemistry Department Scholarship (Baylor University)

1988, 1989 Baylor University Undergraduate Research Symposium, Winner

1989 Top Scholar, University School (Baylor University)

1990 Phi Beta Kappa (Baylor University)

163

1990 American Institute of Chemists Top Senior Chemistry Major (Baylor University)

1990 B.S. with Honors (Honors Research Program) and Magna cum laude (Baylor University)

1990-1994 Iowa Fellow (awarded by The University of Iowa Graduate College to outstanding Ph.D. applicants)

1991 National Science Foundation Graduate Research Fellowship, Honorable Mention (The University of Iowa)

1995-1999 W. M. Keck Computational Biology NLM Postdoctoral Fellow, Rice University and The University of Houston

1996 Professor Clarence P. Berg Award for Outstanding Thesis, Department of Biochemistry, The University of Iowa

5/2001 6th Annual Structural Biology Symposium (Sealy Center, UTMB Galveston) Award for best postdoctoral poster

2002 Gulf Coast Consortium for Bioinformatics/Keck Center for Computational and Structural Biology 2002 Bioinformatics Symposium. 3rd place poster award, Rice University

2005-2006 Who's Who in Science and Engineering

2005 KUMC Searle Scholar nominee

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IV. TEACHING ACTIVITIES (Evaluations and other evidence of quality teaching must be attached) Brief statement of areas of teaching interest: I teach basic biochemical principles to M1 students. My goal is for these students to learn and apply basic principles and reasoning techniques, rather than memorizing long lists of details. For example, when introducing enzymes, I show the students how they can (1) learn the 6 basic chemistries carried out by enzymes (and indicated by each enzyme name) and co-enzymes, and then (2) use that information to rationalize what the reactants and products of a given reaction should be. I point out that this is not just useful for passing the STEP exam, but in an era of individualized patient medicine, MDs might receive a list of altered metabolites and a list of altered proteins for each patient; physicians will need to make educated correlations between the two lists and rationale hypotheses as to whether changes could be related to the presenting symptoms. In another example from lectures on membrane transport, I have these students correlate changes in water, sodium, and glucose uptake from the gut, in order to understand various causes and remedies for some diarrheas. In teaching laboratory research skills, such reasoning is equally important. In general, my strategy has been to allow each student to work as independently as possible, coming to me when they are “stuck” and giving me their interpretation of results before hearing mine. This of course depends on their individual progress, and summer undergraduate students require more guidance (from me or other lab members) than do post-doctoral associates. At all levels, I expect the student to place his or her work in the context of the larger goal of my lab’s research. Opportunities to show this expertise are given in lab meetings and summary reports (i.e. that of the American Chemical Society Project SEED program for economically disadvantaged high school students.) While I design experiments for high school and undergraduate students, I expect graduate students to assume more responsibility for experimental design over the course of their training, and post-doctoral associates even more so. I expect graduate students to write first drafts of their results for publications, and I expect post-doctoral associates to be full participants in the writing process.

Student honors: Dan Parente: Grisolia Award for Excellence in Biomedical Research by a Medical

Student, 2011

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4. Instruction:

Didactic (e.g.: lectures and formal presentations)

Academic Year Course Title

Instruction Student

Type Hours No Type

2006-2007 Foundations of Medicine

Lecturer Lecture 9 ~180 M1

2007-2008 Foundations of Medicine

Lecturer Lecture 13 186 M1

2008-2009 Foundations of Medicine

Lecturer Lecture 14 181 M1

2008-2009 Foundations of Medicine

Co-lecturer

Lecture “Integration of concepts”

3 181 M1

2009-2010 Foundations of Medicine

Lecturer Lecture 14 177 M1

2009-2010 Foundations of Medicine

Co-lecturer

Lecture “Integration of concepts”

3 177 M1

2010-2011 Foundations of Medicine

Lecturer Lecture 14 179 M1

2010-2011 Foundations of Medicine

Co-lecturer

Lecture “Integration of concepts”

3 179 M1

2011-2012 Foundations of Medicine

Lecturer Lecture 14 198 M1

2011-2012 Foundations of Medicine

Co-lecturer

Lecture “Integration of concepts”

3 198 M1

2012-2013 Foundations of Medicine

Lecturer Lecture 13 216 M1

2012-2013 Foundations of Medicine

Co-lecturer

Lecture “Integration of concepts”

3 216 M1

166

Nondidactic (e.g.: workshops, labs, and discussion groups)

Academic Year Course Title

Instruction Student

Type Hours No Type

2005-06 Med Biochem facilitator small group discussions 20

Group 1 = 6

Group 2 = 7

M1

2005-06 Med Biochem facilitator problem-based learning

4 9 M1

2006-07 Foundations of Medicine facilitator small group

discussions 12

Group 1 = 9

Group 2 = 9

M1

2006-07 Foundations of Medicine facilitator large group

discussion 4 ~90 M1

2006-07 Genetics/Neo. facilitator small group discussions 4

Group 1 = 9

Group 2 = 9

M1

2006-07 Foundations of Medicine remediation

one-on-one discussion 3 1 M1

2007-08 Foundations of Medicine facilitator small group

discussions 16

Group 1 = 9

Group 2 = 9

M1

2007-08 Genetics/Neo. facilitator small group discussions 4

Group 1 = 9

Group 2 = 9

M1

2008-09 Foundations of Medicine facilitator small group

discussions 8

Group 1 = 9

Group 2 = 9

M1

2009-10 Foundations of Medicine facilitator small group

discussions 8 Group 1 = 9 M1

167

Group 2 = 9

2009-10 Genetics/Neo. facilitator small group discussions 4

Group 1 = 9

Group 2 = 9

M1

2010-11 Foundations of Medicine facilitator small group

discussions 8

Group 1 = 8

Group 2 = 11

M1

2010-11 Genetics/Neo. facilitator small group discussions 4

Group 1 = 8

Group 2 = 11

M1

2011-12 Foundations of Medicine facilitator small group

discussions 8

Group 1 = 10

Group 2 = 11

M1

2011-12 Genetics/Neo. facilitator small group discussions 4

Grp 1 = ~10

Grp 2 = ~10

M1

2011, fall Special Topics in Biochemistry

Co-director

Supervise independent study for Ja-son Barnett

6 1 Graduate

2012-13 Foundations of Medicine facilitator small group

discussions 8

Group 1 = 12

Group 2 = 12

M1

2012-13 Genetics/Neo. facilitator small group discussions 4

Group 1 = 12

Group 2 = 12

M1

2012, fall Special Topics in Biochemistry

Co-director

Supervise independent study for Mary Ashley Rimmer

2 1 Graduate

168

2013, spring

Special Topics in Biochemistry

Director Supervise independent study for Allen Chazelle

1 1 Graduate

Master’s Theses and PhD Dissertations directed

Year Student Name Thesis Title

Degree (Complet

ed/In process)

2005-2006

Cole Stephens Binding of regulatory molecules to transcription regulatory proteins

Student withdrew

2010 - present

Daniel Parente TBD MD/PhD In progress

Supervision of Postdoctoral Fellows

Year Fellow Name Area of Study

2005-2007, 2009

Hongli Zhan protein structure/function relationship for transcription repressors

Advising (Thesis or dissertation committees: student academic group/individual)

Date Student or group name Type of Student/group

2004 Stephanie Fiedler IGPBS first year rotation

2005 Cole Stephens KU-Edwards Undergraduate research

2005-2009 Benjamin Weaver Comprehensive and Ph.D. thesis committees (BMB, Andrews lab)

2006-2008 Christina Newport Hester

Ph.D. thesis committee (Micro Immunol, Lutkenhaus lab)

2006 Melissa Ruhlman Rockhurst University undergraduate summer student

2006 Jennifer Lamoureux Rockhurst University undergraduate summer student

2006 Tamara Jimenez IGPBS first year rotation

2007, 2008 Linh Thai American Chemical Society Project SEED

169

Summer Research Internship Program for Economically Disadvantaged High School Students

2008 Reggie Nguyen Rockhurst University undergraduate summer student

2008 - 2012 Kristina Bridges Ph.D. thesis committee (Micro Immunol, Zueckert lab)

2008 - 2011 Kendall Smith Comprehensive and Ph.D. thesis committees (MD/PhD in Mol Physiol, Hawley lab)

2009 - present Bria Wilkins Comprehensive and Ph.D. thesis committees (Microbiology, KU-Lawrence, Egan lab)

2009 Mark Wooley Rockhurst University undergraduate summer student

2009 Daniel Parente MD/PhD summer rotation

2009 fall Renae Springe Practicum for MS in Molecular Biotechnology, Dept. of Clinical Laboratory Sciences

2009 fall Scott Tan High school volunteer student

2010 - present Shuai Lu Comprehensive and PhD thesis committees (BMB, Gerton lab)

2010 - present Veerendra Koppolu Comprehensive and Ph.D. thesis committee (Microbiology, KU-Lawrence, Egan lab)

2010 - 2011 Joshua Riepe Rockhurst University undergraduate student

2010 fall Evan Janzen IGPBS first year rotation

2010 - present Jacob Hessman KU-Edwards Undergraduate research

2011 – present Shishen Du Ph.D. thesis committee (Micro Immunol, Lutkenhaus lab)

2012 winter Jessica Shoop IGPBS first year rotation

2012 Feb Mary Ashley Rimmer IGPBS extra rotation

2012 spring Cassandra Field IGPBS first year rotation

2012 spring Allen Chazelle IGPBS first year rotation

2012 summer Bharadwaj Thirumalai High school student

2012 fall Jimmy Budjiardo KU-Lawrence, Program in Bioinformatics,

170

rotation

2012 fall Beth Jaskiewicz IGPBS first year rotation

2013 spring Matthew Stroh IGPBS first year rotation

2013 - present Saswati Biswas Comp. exam committee (Micro Immunol, Yankee lab)

2013 spring Murial Eaton Blue Valley ISD High school CAPS program, independent Biosciences research, outside mentor (teacher JP Whalen)

Other teaching activities

Date Title Place Teaching Function

2004-present presenter The University of Kansas Medical

School

Present opportunities for research in my lab

to 1st year IGPBS students each fall

semester

Summer 2005 advisor The University of Kansas Medical

School

Advise Wyandotte HS science teacher Mr.

Tyrone Bates on summer project,

develop adaptations to use in his classroom

Sept 21, 23, 2005

advisor The University of Kansas Medical

School

Met with 9 Wyandotte HS students to teach

them procedures to be adapted for their HS

classroom; with science teacher Mr.

Bates

May 19, 2006 organizer The University of Kansas Medical

School

SOM graduate student meeting with Dr.

Andrew Robertson to discuss career

opportunities (~15 students)

2006-2007 collaborator Missouri State University

Developed content for a research-based

course with Dr. Colette Witkowski; lectures (2006, 2007) to her

class over-viewing the research project

171

2009-present Graduate Director Dept. of Biochemistry

and Molecular Biology, KUMC

Advise and monitor progress of BMB graduate students

Spring 2011 Mentor, grant writing class

The University of Kansas Medical

School

Comment on presentations and

grant application by graduate student

Subhashchandra Naik

5. Development of Educational Materials (Course materials e.g. syllabi, educational software packages, web sites, films, educational tapes and evaluation tools)

Year Title Description Intended Audience

2006 Foundations of Medicine web activity “ELISA” M1

2007 Foundations of Medicine web activity “ELISA” M1

2007 Foundations of Medicine small group discussion “Cardiac Biomarkers”

M1

2008 Foundations of Medicine web activity “Weak acids”

M1

2008 Foundations of Medicine web activity “Erythrocyte membranes”

M1

2008 Foundations of Medicine web activity “ELISA” M1

2009 Foundations of Medicine web activity “Weak acids”

M1

2009 Foundations of Medicine web activity “Erythrocyte membranes”

M1

2009 Foundations of Medicine web activity “ELISA” M1

2010 Foundations of Medicine web activity “Weak acids”

M1

2010 Foundations of Medicine web activity “Erythrocyte membranes”

M1

2010 Foundations of Medicine web activity “ELISA” M1

2010 Foundations of Medicine web activity “Ischemia/Reperfusion”

M1

2010 Foundations of Medicine web activity “Enzymology”

M1

172

2011 Foundations of Medicine web activity “Weak

acids” M1

2011 Foundations of Medicine web activity “Erythrocyte membranes”

M1

2011 Foundations of Medicine web activity “ELISA” M1

2011 Foundations of Medicine web activity “Ischemia/Reperfusion”

M1

2011 Foundations of Medicine web activity “Enzymology”

M1

2012 Foundations of Medicine web activity “Amino acids”

M1

2012 Foundations of Medicine web activity “Weak acids”

M1

2012 Foundations of Medicine web activity “Erythrocyte membranes”

M1

2012 Foundations of Medicine web activity “ELISA” M1

2012 Foundations of Medicine web activity “Ischemia/Reperfusion”

M1

2012 Foundations of Medicine web activity “Enzymology Review”

M1

2012 Foundations of Medicine web activity “Fluid Mosaic Model”

M1

6. Educational Leadership (Responsibility for courses and other leadership activities including mentoring of junior faculty. Please list faculty members mentored – letters from mentorees may be provided.)

Peer teaching evaluation for Charlotte Vines, Ph. D. (KUMC, Dept. of Microbiology and Immunology), May 2009

Consulting with Sarah Bondos, Ph.D. (Texas A&M Health Science Center) about lecture materials for teaching first year medical students, summer 2009.

2009-2010 As BMB Graduate Director, led reform of BMB graduate student curriculum in response to changes in first year IGPBS curriculum.

173

V. SERVICE ACTIVITIES

Academic Service:

National and regional service

Biophysical Society, Committee for Professional Opportunities for Women (2013-present)

Ad hoc manuscript reviewer for the following journals:

Archives of Biochemistry and Biophysics 2009, 2011

Biochemistry 2004, 2005, 2006, 2009, 2011 (2 reviews), 2012 (2 reviews)

Biochimica et Biophysica Acta, 2011

Biophys J 2010. 2012

BMC Microbiology 2008

FEBS Letters (Federation of European Biochemical Societies) 2005

J. Amer. Chem. Soc. 2010

J. Biomolecular Structure and Dynamics 2008

J. Molecular Biology 2007, 2009 (4 reviews), 2010, 2011, 2012 (2 reviews)

Molecular Microbiology 2011, 2012 (2 reviews)

Nucleic Acids Research 2012

PLoS1 2009

pLoS Computation Biology 2013

Protein Engineering, Design, and Selection 2008, 2009

Protein Science 2007, 2010

Proteins: Structure, Function, Bioinformatics 2006, 2008

Trends in Biochemical Sciences 2008

NIH, Macromolecular Structure and Function B study section. 6/23-24/2005. Ad hoc reviewer (12 proposals)

NSF ad hoc reviewer

2006, Microbial Genetics (2 proposals)

2009, Molecular and Cellular Biophysics (1 proposal)

Cellular Organization (1 proposal)

2011, Molecular and Cellular Biophysics (2 proposals)

174

Netherlands Organisation for Scientific Research, ad hoc reviewer

2009, Division for Chemical Sciences (1 proposal)

Johnson County Community College, Biotechnology Advisory Committee; 5/2006-2009

Twice-yearly committee meetings to advise JCCC on areas related to their biotech education program

COBRE in Protein Structure and Function (KU-Lawrence)

Protein Purification Core Steering Committee, 2008-present

Hire and oversee director and staff for a facility that provides protein purification services to the Kansas research community

Conference Organizer

The 23rd Annual Gibbs Conference in Biothermodynamics, Oct 3-6, 2009, Carbondale, IL

Reviewer, National Research Council, Research Associateship Program

May 2011 – 3 proposals

Sept 2011 – 7 proposals

May 2012 – 3 proposals

Sept 2012 – 6 proposals

Dec 2012 – 1 proposal

Outside reviewer for promotion and tenure:

Northern Illinois University, Department of Chemistry and Biochemistry, 2011

Session organizer: “Saturday Night Thermo” for graduate students and post-docs

The 25th Annual Gibbs Conference in Biothermodynamics, Sep 17, 2011

The 26th Annual Gibbs Conference in Biothermodynamics, Sep 22, 2012 The 27th Annual Gibbs Conference in Biothermodynamics, Oct 5, 2013

Judge, student poster presentations.

2012 Biochemistry and Cell Biology 40th Anniversary Reunion Symposium, Rice University, Houston, TX

175

The University of Kansas Medical School service Biochemistry and Molecular Biology

Presentation of BMB research opportunities to 1st year IGPBS students

2004, 2005, 2006, 2007, 2008, 2009, 2010, 2011, 2012

Wrote justifications and participated in purchasing equipment for the Proteomics Center, 2004

Chair, Graduate recruiting committee, 2005-2009

Developed departmental procedure for contacting and tracking prospective graduate students

Updates to departmental website

Updates to departmental brochure

Recruiting booth at regional American Chemical Society meeting (KC, MO November, 2007)

Graduate Education Committee, 2005-present

Kimmel Award for Graduate Research, award committee, 2005

Internal grant reviews, 2005-2006

Promotion and Tenure Committee, 2009 - present

Departmental website coordinator, 2010 - present

School of Medicine

Member, junior faculty panel, LCME accreditation visit, 2005

LCME self-study, Medical Student Services subcommittee, 2012

Judge, Student Research Forum

April 2006, 1 section

April 2007, 2 sections

April 2008, 2 sections

176

IGPBS student recruiting weekend.

Presentation (overview of proteomics and structural biology at KUMC) and interviews, 2005

Presentation (overview of proteomics and structural biology at KUMC) and poster, 2007

Presentation (overview of proteomics and structural biology at KUMC), interviews, and poster, 2008

Recruiting and interviews, 2010

Organized 2012 weekend as IGPBS Assistant Director

Organized 2013 weekend as IGPBS Assistant Director

IGPBS Admissions Committee and interviews, 2009, 2011

IGPBS Advisory Board, fall 2009 - present

MD/PhD student recruiting visits

3 presentations overviewing proteomics and structural biology at KUMC, 2007

3 presentations overviewing proteomics and structural biology at KUMC, student interviews, 2008

MD/PhD Admissions Committee, fall 2008-present

Guide for KLSIC building tours, Jan 26, 2007

Selection committee for Ruth T Bohan teaching award, 2007

Wahl Society mentor for medical students, 2006-present

Biomedical Research Training Program, application review panel, May 2009, May 2010

WIMS (Women in Medicine and Science) departmental mentoring delegate, 2011-present

Department of Pharmacology and Toxicology, Search Committee for Departmental Chair, 2011

177

Academic and Professionalism Committee:

Student Promotions and Special Programs Subcommittee, 2011 – present

Vice chair, 2012-2013

MD/PhD Annual Retreat, July 13, 2012, Presentation on experiences with patents to students

School of Allied Health

Dept of Clinical Laboratory Sciences, Faculty Search Committee, winter 2007-2008

KUMC

Discussion with BIRCHW* scholars on grant budgeting, June 29, 2012

(*”Building Interdisciplinary Research Careers in Women's Health”)

Panelist for the discussion on Open Access Publishing, Oct 25, 2012

Community service

KC, KS Saturday science club, “Meet the mentor” with high school students, Feb 26, 2005

Westwood View Elementary School Science Night; 2005, 2006, 2007, 2008, 2009, 2010, 2011, 2012, 2013

Develop, supervise facilitators, and lead 8 hands-on activities for ~100 students

Presentations to PEO (a women’s philanthropic educational organization)

Give scientific background for current scientific/ethical issues and lead discussion

April 11, 2006; August 11, 2007; Mar 10, 2009; Apr 2011; March 2012; spring 2013

Blue Valley ISD, Center for Advanced Professional Studies Bioscience Program, speaker

Mar 24, 2011; Oct 10, 2011; Oct 18, 2012

Rockhurst University, Biotechnology course, guest lecturer

Nov 8, 2011

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VI. RESEARCH and SCHOLARLY ACTIVITIES:

Brief statement of areas of research and scholarly interest, including current projects: In sequence alignments of protein families, amino acid residues fall into three groups. Conserved residues convey a common function (e.g. DNA-binding). Nonconserved residues either fine-tune the distinct functions of homologous family members (e.g. recognition of different DNA sequences) or are “silent” (mutagenesis has no impact). Understanding the link between amino acid variation of nonconserved residues and variation in protein function is important for protein engineering and developing individualized patient therapies. These applications are impeded by difficulties in discriminating important nonconserved residues from silent residues. To differentiate these groups, myriad bioinformatics algorithms have been recently developed. The algorithms rely upon the untested assumption that a given site falls into a given group for every homologue. Further, the algorithms cannot predict which aspect of function is altered by each nonconserved position. For example, amino acid variation in a transcription repressor might alter affinity or selectivity for DNA or allosteric response to regulatory ligand.

My research bridges bioinformatics, biochemistry, and biophysics to identify important nonconserved residues. Using transcription repressors, we characterize in vivo function of multiple variants for multiple homologues. Chimeric proteins are used to simultaneously facilitate comparisons between homologues and to investigate contributions from residues that do not directly contact DNA. Results from mutagenesis test predicted locations, discover trends among homologues, and suggest improved strategies for sequence analyses. Biophysical experiments delineate which aspect of function is linked to each nonconserved residue and determine whether these roles are conserved among homologues. Future studies will extend to other protein families.

A second project is involved in determining whether a protein complex between the Cra transcription regulator and the FruK enzyme is a novel antibiotic target against γ-proteobacteria.

In addition to students, my lab has included a number of research scientists: Sarah Meinhardt, BS (2005-2013); Sudheer Tungtur, MS/MS (2006 - 2012); Ara Azad, BS (2007); Michael Manley, BS (2009 - present); Renae Springe, BS (2010).

179

4. Grants and contracts

(Information must include whether the nominee is the principal investigator or a co-investigator, names of all investigators, title of grant, funding source, dollar amount in direct costs, and years during which grant applies. Co-investigators must specify role). Provide four copies of abstracts and award notice or renewal for all grants or contracts awarded in last five years. Grants and contracts awarded: (List in chronological order)

Principal Investigator Investigators Title of Grant

Funding Source

Direct Costs Years Status

Hanzlik, Robert (KU-

Lawrence)

Michaelis, M Co-PI (KU-Lawrence)

Swint-Kruse (Investigator Subaward)

COBRE: Protein

Structure and Function

Subaward: Allosteric

determinants across the LacI/GalR

family

NIH/NCRR $300,000 to LSK

9/1/04-6/30/07

Completed

Swint-Kruse

KUMC travel award

KUMC $350 Jan 2005 Completed

Swint-Kruse (PI)

Functional effects of

exchanging domains and

linkers in transcription regulators

NIH/NIGMS $900,000 8/1/07-7/31/12

n.c.e. to

7/31/13

Funded

Swint-Kruse

KUMC travel award

KUMC $250 Jul 2007

Completed

Swint-Kruse

(PI)

ARRA Administrative Supplement

to: Functional effects of

exchanging domains and

linkers in transcription regulators

NIH/NIGMS $179,626 8/1/2009- 7/31/2011

Completed

Swint-Kruse

KUMC travel award

KUMC $300 Feb 2013 Completed

180

Grants and contracts submitted:

Principal Investigator Investigators Title of Grant

Funding Source

Direct Costs Years Status

Swint-Kruse

Intraprotein signal

transmission in Venus-flytrap

domains

NIH $1,125,000 4/1/06-3/3/11

Not funded

Swint-Kruse

Identifying determinants of unique function

in a family of transcription regulatory proteins

Searle Scholars Program

$240,000 6/1/06-5/31/08

Not funded

Swint-Kruse

Functional contributions of domain linkers in engineered transcription regulatory proteins.

NSF $552,000 2/1/07-1/31/12

Not funded

Swint-Kruse

Young Investigator

Award

Howard Hughes Medical Institute

$2,000,000 2009-2015 Not funded

Egan, Susan (KU-Lawrence)

Co-PI

Swint-Kruse

Design and guide

experiments of Aim 3

Transcription Activation at the rhaBAD

operon

NIH $21,000 to LSK

2011-2015 Oct 2009: 30%ile

June 2010:

22%ile

Not funded

Swint-Kruse

Identification of functional

properties in common to

members of a protein family

NIH $1,359,507 8/1/2012-7/31/2017

A0: Not

funded

A1: Not

181

funded

Swint-Kruse and

Aron Fenton

Swint-Kruse and Fenton

Protein polymorphisms: Distinguishing unimportant

from disease-causing

mutations

NIH $1,000,000 9/1/2012-8/31/2016

Not funded

Swint-Kruse

Experimental characterization

of nonconserved

protein positions: Key

data for advancing

bioinformatics analyses

NSF $720,000 1/1/2013 – 12/31/2016

Not funded

Susan Egan

PI: Joe Lutkenhaus

Swint-Kruse

collaborator

COBRE: Novel

Approaches for Control of

Microbial Pathogens

Pilot project:title

NIH Cost to LSK

01/01/2013– 06/30/2013

Not funded

Swint-Kruse

The Cra-FruK complex: A new antibiotic target

in gamma-proteobacteria

NIH $1,250,000 9/1/2013-8/31/2018

In review

182

5. Scholarly Publications

Full length, peer-reviewed articles in scientific journals: (Provide names of all authors, year, title, journal, volume, and inclusive pages. List in chronological order.)

Articles published:

Authors Year Title Journal Vol Pages

Swint, L. and A. D. Robertson

1993 "Thermodynamics of unfolding for turkey ovomucoid third domain: thermal and

chemical denaturation."

Protein Sci 2 2037-49

Swint-Kruse, L. and A. D. Robertson

1995 "Hydrogen bonds and the pH dependence of ovomucoid third domain

stability."

Biochemistry 34 4724-32

Swint-Kruse, L. and A. D. Robertson

1996 "Temperature and pH dependences of hydrogen exchange and global stability

for ovomucoid third domain."

Biochemistry 35 171-80

Falcon, C. M., Swint-Kruse, L. and K. S.

Matthews

1997 "Designed disulfide between N-terminal domains of lactose repressor disrupts

allosteric linkage."

J Biol Chem 272 26818-21

Swint-Kruse, L., K. S. Matthews, P. E. Smith

and B. M. Pettitt

1998 "Comparison of simulated and experimentally determined dynamics for

a variant of the Lacl DNA-binding domain, Nlac-P."

Biophys J 74 413-21

Swint-Kruse, L.*, C. R. Elam, J. W. Lin, D. R. Wycuff and K. Shive

Matthews

*Corresponding author

2001 "Plasticity of quaternary structure: twenty-two ways to form a LacI dimer."

Protein Sci 10 262-76

Swint-Kruse, L.*, C. Larson, B. M. Pettitt and

K. S. Matthews

*Corresponding author

2002 "Fine-tuning function: correlation of hinge domain interactions with functional

distinctions between LacI and PurR."

Protein Sci 11 778-94

Flynn, T. C.*, Swint-Kruse, L.*, Y. Kong, C. Booth, K. S. Matthews

and J. Ma

*Co-first authors

2003 "Allosteric transition pathways in the lactose repressor protein core domains: asymmetric motions in a homodimer."

Protein Sci 12 2523-41

Swint-Kruse, L., H. Zhan, B. M. Fairbanks, A. Maheshwari and K.

S. Matthews

2003 "Perturbation from a distance: mutations that alter LacI function through long-

range effects."

Biochemistry 42 14004-16

Swint-Kruse, L. 2004 "Using networks to identify fine structural differences between functionally distinct

Biochemistry 43 10886-

183

protein states." 95

Swint-Kruse, L. and K. S. Matthews

2004 "Thermodynamics, protein modification, and molecular dynamics in

characterizing lactose repressor protein: strategies for complex analyses of

protein structure-function."

Methods Enzymol

379 188-209

Swint-Kruse, L.* and C. S. Brown

*Corresponding author.

2005 "Resmap: automated representation of macromolecular interfaces as two-

dimensional networks."

Program download has been requested 72 times as of Feb 15, 2011.

Bioinformatics 21 3327-8

Swint-Kruse, L.*, H. Zhan* and K. S.

Matthews

*Co-first authors

2005 "Integrated insights from simulation, experiment, and mutational analysis yield new details of LacI function."

Biochemistry 44 11201-13

Zhan, H., Swint-Kruse, L. and K. S. Matthews

2006 "Extrinsic interactions dominate helical propensity in coupled binding and

folding of the lactose repressor protein hinge helix."

Biochemistry 45 5896-906

Kolin, A., V. Jevtic, Swint-Kruse, L. and S.

M. Egan

2007 "Linker regions of the RhaS and RhaR proteins."

J Bacteriol 189 269-71

Tungtur, S., S. M. Egan and L. Swint-Kruse

2007 "Functional consequences of exchanging domains between LacI and PurR are mediated by the intervening

linker sequence."

Proteins 68 375-88

Wilson, C. J., H. Zhan, L. Swint-Kruse and K.

S. Matthews

2007 "Ligand interactions with lactose repressor protein and the repressor-

operator complex: the effects of ionization and oligomerization on

binding."

Biophys Chem 126 94-105

Swint-Kruse, L. and H. F. Fisher

2008 "Enzymatic reaction sequences as coupled multiple traces on a multidimensional landscape."

Trends Biochem Sci

33 104-12

Taraban, M., H. Zhan, A. E. Whitten, D. B.

Langley, K. S. Matthews, L. Swint-

Kruse* and J. Trewhella*

*Co-corresponding authors

2008 "Ligand-induced conformational changes and conformational dynamics in the

solution structure of the lactose repressor protein."

J Mol Biol 376 466-81

184

Zhan, H., M. Taraban, J. Trewhella and L. Swint-

Kruse

2008 "Subdividing repressor function: DNA binding affinity, selectivity, and allostery

can be altered by amino acid substitution of nonconserved residues in

a LacI/GalR homologue."

Biochemistry 47 8058-69

Meinhardt, S. and L. Swint-Kruse

2008 "Experimental identification of specificity determinants in the domain linker of a

LacI/GalR protein: Bioinformatics-based predictions generate true positives and

false negatives."

Proteins 73 941-57

Tungtur, S., Meinhardt, S., and Swint-Kruse, L.

2010 “Comparing the functional roles of nonconserved sequence positions in homologous transcription repressors:

Implications for sequence/function analyses.”

J. Mol Biol 395 785-802

Tungtur, S., Parente, D.J., and Swint-Kruse,

L.

2011 “Functionally Important Positions Can Comprise the Majority of a Protein’s

Architecture.”

Proteins 79 1589-1608

Tungtur, S., Skinner, H., Zhan, H.L., Swint-

Kruse*, L., Beckett, D.*

*Co-corresponding authors

2011 In vivo tests of thermodynamic models of transcription repressor function.

Biophysical Chemistry

159 142-151

Meinhardt, S., Manley, M.W. Jr., Becker, N.A., Hessman, J.A., Maher,

J.L. III, and Swint-Kruse, L.,

2012 Novel insights from hybrid LacI/GalR proteins: Family-wide functional

attributes and biologically significant variation in transcription repression.

Nucleic Acids Research

40 11139-54

Manuscripts in press: (Provide names of all authors, title, journal, and 4 photocopies of manuscript plus evidence of acceptance by journal)

Authors Title Journal

Manuscripts submitted but not yet accepted: (Provide names of all authors, title, journal, and 4 photocopies plus evidence of receipt of manuscript by journal.)

Authors Title Journal

185

Invited or non-peer-reviewed articles or reviews: (Provide names of all authors, year, title, journal, volume, and pages. If in press, provide documentation and 4 photocopies if published within the last five years.)

Authors Year Title Journal Vol Pages

Matthews, K. S., C. M. Falcon and Swint-

Kruse, L.

2000 "Relieving repression."

Nature Structural Biology 7 184-7

Wilson, C. J., H. Zhan, L. Swint-Kruse and K.

S. Matthews

2007 "The lactose repressor system:

paradigms for regulation, allosteric behavior and protein

folding."

Cell Mol Life Sci 64 3-16

Swint-Kruse, L.* and Matthews, K. S.

*Corresponding author

2009 Allostery in the LacI/GalR Family: Variations on a Theme

Current Opinion in Microbiology

12 129-137

Books and book chapters: (Provide names of all authors, year, book title, chapter title, edition, publisher, and pages. If in press, provide documentation and 4 photocopies if published the last five years.)

Authors Year Book Title Chapter Title Edition Publisher Pages

Bondos, S., Swint-Kruse, L., & Matthews, K.

2000 McGraw-Hill Yearbook of Science and Technology 2000

“Protein Folding”

Licker, M., publisher, McGraw-Hill, New York

361-363

Swint-Kruse, L. and Matthews K. S.

2004 Encyclopedia of Biological Chemistry

“Gene expression in bacterial systems: lac operon.”

2 W.J. Lennarz and M.D.Lane, eds., Academic Press, San Diego, CA.

529-535

Swint-Kruse, L in: Krebs, JE, Goldstein, ES. and Kilpatrick, ST, eds

2011 Lewin's Genes X

Chapter Editor for Chapter 26: “The Operon”

10 Jones and Bartlett, publishers, Sudbury, MA

pp 735-766.

186

Swint-Kruse, L. and Matthews K. S.

2012 Encyclopedia of Biological Chemistry

Revisions to: “Gene expression in bacterial systems: lac operon.”

Volume 2

W.J. Lennarz and M.D.Lane, eds., Academic Press, San Diego, CA.

pp 529-534

Published abstracts: (Provide names of all authors, year, title, where published, volume, and pages.)

Authors Year Title Where published Volume Pages

Swint, L. and A. D. Robertson

1994 "Correlation of global and local stabilities of ovomucoid 3rd

domain."

Biophysical Journal 66 A181

SwintKruse, L., B. M. Pettitt and K. S.

Matthews

1997 "Design and tests of conformational change for a

transcriptional repressor protein."

Biophysical Journal 72 TH270

Zhan, H. L., L. Swint-Kruse, B. Fairbanks and K. S. Matthews

2003 "Mutations that affect allosteric transition in lactose repressor."

Biophysical Journal 84 501A

Matthews, K. S., L. Swint-Kruse, J.

Barry, C. Falcon, J. P. Ma and H. L. Zhan

2004 "Lactose repressor protein allosteric behavior: Role of

flexibility."

Biophysical Journal 86 354A

Zhan, H. L., L. Swint-Kruse and K. S.

Matthews

2004 "Mutations that affect allosteric regulation in LacI."

Biophysical Journal 86 81A

Zhan, H. L., L. Swint-Kruse and K. S.

Matthews

2005 "Blocking the path: Hydrophobic mutations at position 84 impair

inducibility in the lactose repressor protein."

Biophysical Journal 88 220A-221A

Swint-Kruse, L., H. L. Zhan, S. Tungtur and

S. Meinhardt

2007 "Functional effects of exchanging domains between homologous proteins are mediated by the

linker."

Biophysical Journal Supple-ment S

219A-

Taraban, M., H. L. Zhan, K. Matthews,

L. Swint-Kruse and J. Trewhella

2007 "Small angle scattering studies of LacI repressor."

Biophysical Journal Supple-ment S

52A

187

Swint-Kruse, L., Zhan, H., and

Tungtur, S

2008 “Determinants of unique repressor function”

Biophysical Journal 94

Supple-ment

Pos 2722

Swint-Kruse, L., Manley, M., Tungtur,

S., and Zhan, H.L

2011 Correlating in vitro measurements of protein-DNA binding affinities

with in vivo repression and impact on the growth rate of the host

organism.

Biophysical Journal 100(3), S1

321a

Swint-Kruse, L., and Parente, D.J.

2012 Multiple co-evolutionary networks have evolved on the common

tertiary scaffold of the LacI/GalR proteins.

Biophysical Journal 102(3), S1

184a

Swint-Kruse, Tungtur, Zhan,

Becker, Maher, and Riepe

2013 In vitro thermodynamics of DNA binding correlate with in vivo transcription repression by a synthetic LacI/GalR paralog

Biophysical Journal 102(4)

S1

xx

Other scholarly publications N/A

6. Presentations and posters Oral paper presentations: (Provide names of all authors, title, sponsoring organization, extent of peer-review, and location and date of presentation.)

Scientific papers presented at national and international meetings:

Since September, 2003

Asymmetric Allosteric Transition Pathway in the LacI Core Domain Homodimer

17th Annual Gibbs Conference in Biothermodynamics, Carbondale, IL, Sept 29, 2003.

Invited by organizers.

Subdividing repressor function: DNA binding affinity, selectivity, and allostery can be altered by amino acid substitution of nonconserved residues in a LacI/GalR homologue

22nd Annual Gibbs Conference in Biothermodynamics, Carbondale, IL, October 5, 2008

Invited by organizers.

Subdividing repressor function.

64th Calorimetry Conference, Santa Fe, NM, Jun 28-Jul 2, 2009. Invited by organizers.

188

Comparing the functional roles of nonconserved sequence positions in homologous transcription repressors: Implications for sequence/function analyses

Keystone symposium, Structural Genomics, Breckenridge, CO. Jan 10, 2010. Invited by

organizers from submitted abstract.

Bringing Science to the Layperson.

09/27/10 The 24th Annual Gibbs Conference on Biothermodynamics, Carbondale, IL.

Chosen from submitted abstracts.

Experimental Description of a Protein Family: Critical experiments for the post-genomic era

09/25/12 The 26th Annual Gibbs Conference on Biothermodynamics, Carbondale, IL.

Invited by the organizers.

Scientific papers presented at local and regional meetings: N/A Poster presentations: At national and international meetings: From April 2004 2/12/05-2/16/05 Biophysical Society 49th Annual Meeting, Long Beach, CA. Peer-reviewed. 1. “Blocking the path: Hydrophobic mutations at position 84 impair inducibility in the lactose repressor protein.” Zhan, Swint-Kruse, and Matthews. (1076-Pos) 2. “Using networks to identify functionally relevant differences between similar protein structures.” Swint-Kruse, (pos-L37) 7/30/05-8/03/05 19th Symposium of the Protein Society, Boston, MA. Peer-reviewed. “RESMAP: Automated Representation of Protein Interfaces as Two-Dimensional Networks Aids Detailed Comparison of Multiple Structures.” Swint-Kruse and Brown. (abstract #560).

189

2/18/06-2/22/06 Biophysical Society 50th Annual Meeting, Salt Lake City, UT. Peer-reviewed. 1. Functional consequences of domain swapping between the LacI/GalR transcription regulatory proteins. Swint-Kruse, Zhan, Meinhardt, and Tungtur (1671-Pos) 2. Extrinsic interactions dominate helical propensity in coupled binding and folding of lactose repressor protein hinge helix. Zhan, Swint-Kruse, and Matthews. (2434-Pos) 10-10/06 20th Annual Gibbs Conference in Biothermodynamics, Carbondale, IL. Peer reviewed. “Functional Consequences of Exchanging domains between LacI and PurR: The linker sequence modulates DNA-binding in a manner that is context-dependent upon the regulatory domain.” Swint-Kruse, Zhan, Tungtur, and Meinhardt. 3/2/2007-3/7/2007 Biophysical Society 51st Annual Meeting, Baltimore, MD. Peer reviewed. 1. Functional effects of exchanging domains between homologous proteins are mediated by the linker; Swint-Kruse, Zhan, Tungtur, Meinhardt (1027-Pos). 2. Small Angle Scattering Studies of LacI Repressor; Taraban, Zhan, Woodward, Swint-Kruse, Matthews, and Trewhella (238-Pos). 7/21/2007 - 7/26/2007. American Crystallographic Association Annual Meeting, Salt Lake City, UT. Peer-reviewed. Small Angle Scattering Studies of LacI Repressor. Taraban, Zhan, Matthews, Swint-Kruse, and Trewhella 7/21/2007-7/25/2007 21st Symposium of the Protein Society, Boston, MA. Peer reviewed. Modifying function in engineered transcription repressors. Swint-Kruse and Meinhardt. 9/29/2007 - 10/2/2007 21st Annual Gibbs Conference in Biothermodynamics, Carbondale, IL. Peer reviewed. Determinants of Unique Repressor Function. Swint-Kruse, Zhan, Tungtur, Taraban, and Trewhella. 2/6/2008 Biophysical Society 52nd Annual meeting, Long Beach, CA. Peer reviewed. Determinants of Unique Repressor Function. Swint-Kruse, Zhan, and Tungtur (2722-Pos). 06/11/08 – 06/12/08 Gordon Conference in Biopolymers, Newport, RI. Peer reviewed. Determinants of Unique Repressor Function. Swint-Kruse.

01/08/10 Keystone Symposium: Structural Genomics. Peer reviewed. Comparing the functional roles of nonconserved sequence positions in homologous transcription repressors: Implications for sequence/function analyses. Tungtur, Meinhardt, and Swint-Kruse.

09/26/10 The 24th Annual Gibbs Conference on Biothermodynamics, Carbondale, IL. Peer reviewed. Correlating in vitro

190

measurements of protein-DNA binding affinities with in vivo repression and impact on the growth rate of the host organism. Manley, Tungtur, and Swint-Kruse.

09/27/10 The 24th Annual Gibbs Conference on Biothermodynamics, Carbondale, IL. Peer reviewed. Comparison of the co-evolution networks between subfamilies of the LacI/GalR transcription repressor family. Parente, Tungtur, and Swint-Kruse.

3/7/2011 Biophysical Society 55th Annual meeting, Baltimore, MD. Correlating in vitro measurements of

protein-DNA binding affinities with in vivo repression and impact on the growth rate of the

host organism. Manley, Tungtur, Zhan, and Swint-Kruse.

09/18/11 The 25th Annual Gibbs Conference on Biothermodynamics, Comparison of co-evolutionary

network structure between subfamilies of the LacI/GalR protein family. Parente and Swint-

Kruse.

09/18/11 The 25th Annual Gibbs Conference on Biothermodynamics, Functional contributions of

nonconserved amino acids to homologs. Swint-Kruse and Tungtur.

01/15/12 – 1/20/12 Gordon Conference on Biomolecular Interactions and Meth., Galveston, TX,

Protein polymorphisms at nonconserved positions give rise to changes in in vitro protein-DNA

binding affinities that correlate with altered in vivo repression and organism growth rates.

Swint-Kruse, Manley, Zhan, and Tungtur

02/26/12 Biophysical Society 56th Annual meeting, Multiple co-evolutionary networks have evolved on

the common tertiary scaffold of the LacI/GalR proteins. Swint-Kruse and Parente

09/24/12 The 26th Annual Gibbs Conference on Biothermodynamics, Carbondale, IL. Experimental tests

for the existence of neutral positions in the LacI/GalR family. Parente and Swint-Kruse

02/06/13 Biophysical Society 57th Annual meeting, Philadelphia, PA. In vitro

thermodynamics of DNA binding correlate with in vivo transcription repression by a synthetic

LacI/GalR paralog. Swint-Kruse, Tungtur, Zhan, Becker, Maher, and Riepe.

191

06/16/13 – 06/21/13 Gordon Conference on Proteins, Holderness, NH. Nonconserved amino acids as rheostats for modifying transcription repressor proteins. Swint-Kruse, Meinhart, Manley, and Tungtur.

Poster presentations at local and regional meetings:

From April 2004

11/18/04 Faculty Research Day, KUMC, Kansas City, KS. Not peer reviewed.

“Tools to identify functionally relevant fine details in protein structures.” Swint-Kruse Fall, 2005 Faculty Research Day, KUMC, Kansas City, KS. Not peer reviewed. “RESMAP: Automated Representation of Protein Interfaces as Two-Dimensional Networks Aids Detailed Comparison of Multiple Structures.” Swint-Kruse and Brown 9/30/2006. Midwest Enzyme Chemistry Conference. Chicago, IL. Peer reviewed. “Enzymatic reaction sequences as coupled multiple traces on a multi-dimensional landscape” Fisher and Swint-Kruse 11/7/2007 - 11/9/2007. Midwest Regional American Chemical Society meeting. Kansas City, MO Peer reviewed. Determinants of Unique Repressor Function. Swint-Kruse, Zhan, Tungtur, Taraban, and Trewhella 11/06/2008 Faculty Research Day, KUMC, Kansas City, KS. Not peer reviewed. Determinants of Unique Repressor Function. Swint-Kruse, Zhan, Meinhardt, and Tungtur

11/03/2009 Faculty research day, KUMC. Not peer reviewed. Comparing the functional roles of nonconserved sequence positions in homologous transcription repressors: Implications for sequence/function analyses. Tungtur, Meinhardt, and Swint-Kruse

10/12/10 KUMC, Faculty Research Day. Correlating in vitro measurements of protein-DNA binding

affinities with in vivo repression and impact on the growth rate of the host organism. Manley,

Tungtur, and Swint-Kruse

10/07/12 Symposium on Protein Structure and Function, in conjunction with the 10th anniversary of

the COBRE in Protein Structure and Function, KU-Lawrence. Multiple co-evolutionary

networks have evolved on the common tertiary scaffold of the LacI/GalR proteins. Parente

192

and Swint-Kruse.

10/12/12 Biochemistry and Cell Biology 40th Anniversary Reunion Symposium, Rice University,

Houston, TX. Protein polymorphisms at nonconserved positions give rise to changes in in

vitro protein-DNA binding affinities that correlate with altered in vivo repression and

organism growth rates. Swint-Kruse, Meinhardt, Tungtur, Zhan, and Manley

Invited seminars at other universities:

Since October, 2003

Tools to identify functionally relevant fine details in protein structure.

W. M. Keck Center for Computational Biology Seminar Series, Houston, TX, Spring 2004

KU Med. School, Department of Biochem. Mol. Biol. Seminar Series, Sept 9, 2004

Using Networks to Identify Relevant Differences in Related Protein Structures.

KU-Lawrence COBRE in Protein Structure and Function, Jan 12, 2005

Understanding Allostery in Transcription Regulatory Proteins: Integrating Simulation, Experiment, and Mutational Analyses.

University of Missouri – Kansas City, School of Biological Sciences, Oct 27, 2005

Post-genomic Challenges in Understanding the Protein Structure/Function Relationship.

Missouri State University, Biomedical Sciences Departmental Seminar Series, Apr 28, 2006

Functional effects of exchanging domains and linkers in transcription regulators

KU-Lawrence COBRE in Protein Structure and Function, Nov 15, 2006

Identifying Functional Specificity Determinants for a Family of Transcription Regulators

Kansas State University, Biochemistry Departmental Seminar Series, Feb 14, 2007

Bridging Bioinformatics and Protein Engineering: The Functional Effects of Amino Acid Polymorphisms

Pittsburg State University, Chemistry Departmental Seminar Series, Nov 30, 2007

193

Bridging Bioinformatics and Biochemistry to Engineer Unique Repressor Functions

Texas A&M Health Science Center, College Station, TX, Department of Molecular and Cellular Medicine, Dec 2, 2008

University of Kansas, Lawrence, KS, Department of Molecular Biosciences, Jan 26, 2009

University of Texas Medical Branch, Galveston, TX, Department of Biochemistry and Molecular Biology, Jan 29, 2009

UT Health Science Center, San Antonio, TX, Department of Biochemistry, Sep 11, 2009

Using bioinformatics and protein engineering to elucidate the functional impact of protein polymorphisms

Kansas State University, Manhattan, KS, Department of Biochemistry, Feb 10, 2010

Functional Effects of Amino Acid Polymorphisms: Implications for Individualized Patient Therapies and Protein Engineering

Notre Dame University; Department of Chemistry and Biochemistry, Nov 5, 2010

Park University; Chemistry and Biology clubs, Nov 9, 2010

Wichita State University, Department of Chemistry, Nov 16, 2011

Experimental Description of a Protein Family: Critical experiments for the post-genomic era

Rice University, Department of Biochemistry and Cell Biology, May 14, 2012

Louisiana State University, Department of Biological Sciences, Nov 5, 2012

The LacI/GalR bacterial transcription regulators: Metabolic

surprises from experimental bioinformatics

The University of Kansas Medical Center, Department of Microbiology, Molecular Genetics,

and Immunology, Feb 12, 2013

194

7. Other evidence of scholarship

(includes clinical guidelines, policy documents, contributions to significant position statements by professional organizations, and development of national examinations) Patent: Matthews, K. S., Falcon, C. M., and Swint-Kruse, L. Lactose repressor proteins with increased operator DNA binding affinity. Filed 7/2002 and approved 4/16/2008. Application number 10/197,053.

195

UNIVERSITY OF KANSAS SCHOOL OF MEDICINE

Faculty Curriculum Vitae

Date: May 13, 2013

I. PERSONAL DATA: Instructions available online.

1. Applicant Information:

First Name: Alexey Middle Initial: S Last Name: Ladokhin Suffix: Degree(s): Ph.D., D.Sc. Current Academic Rank: Associate Professor Primary Department: Biochemistry and Molecular Biology Secondary Department: Office Address: 1073 KLSIC Phone: 913-588-0490 Fax: 913-588-9896 Email: aladokhin@kumc.edu

2. Professional Development: Instructions available online.

Undergraduate and Graduate Education: Years (inclusive) Degree Institution

1979-1984

1984-1989

BS (Physics)

Ph.D. (Biophysics)

Shevchenko National Univ., Kiev

Inst. of Biochemistry, NANU, Kiev

Postgraduate Education: Years (inclusive) Degree Institution

1990-1992

1992-1994

1994-2000

Postdoctoral Research Associate

Postdoctoral Fellow

Post-Graduate Researcher

University of Virginia, Charlottesville, VA

Johns Hopkins University, Baltimore, MD

University of California, Irvine, CA

Academic and Professional Appointments and Activities:

196

Month and Year Position Institution

2000-2004

2004-2008

2008-present

Asst. Biophysicist (Equivalent of Research Assistant Professor)

Assistant Professor

Associate Professor

University of California, Irvine, CA

Univ. of Kansas School of Medicine, Kansas City, KS

Univ. of Kansas School of Medicine, Kansas City, KS

Professional Registration/Licensure:

Year Number State

Professional Certification(s):

Date Board

Professional Societies and Affiliations: List by national and local level; include offices held

Date Organization

1989 – present

1990 – present

2011 - present

Ukrainian Biochemical Society

American Biophysical Society

American Chemical Society

Honors and Awards:

Year Award

1984

1988

1992

2002

Diploma with Honors in Molecular Physics, Shevchenko National University, Ukraine

Best Young Scientist Award, Institute of Biochemistry, Kiev, Ukraine

McCollum-Pratt Institute Fellowship, The Johns Hopkins Univ., Baltimore, MD

Doctor of Science Degree, National Academy of Sciences of Ukraine, Kiev, Ukraine

197

II. TEACHING ACTIVITIES: Instructions available online.

Teaching evaluations and other evidence of quality teaching must be provided and should accurately summarize ALL of your teaching activities. The absence of teaching evaluations may adversely affect your application for Promotion and/or Tenure. Philosophy of Teaching: Briefly describe your philosophy of teaching and how you apply it to different types of learners, e.g. medical students, graduate students, residents, fellows.

Although I define my field as molecular biophysics, my research is interdisciplinary and draws upon my knowledge of biochemistry and molecular biology, as well as chemistry and physics. So my teaching has to be multidisciplinary too. My expertise in these fields makes me confidently eager to teach courses as diverse as biochemistry, biophysics, protein structure and thermodynamics, physical methods in biomedical sciences, and membrane protein structure and function. I believe that students should be taught the very latest scientific concepts and that they should be exposed to an historical perspective on current theories (I implement these aspects in my graduate courses). The most important and difficult challenge in teaching is not so much imparting knowledge as demonstrating effectively how that knowledge is obtained from observation, experiment, and analysis.

1. Instruction:

Didactic: e.g. lectures and formal presentations

Academic Year

Title and Course

Number

Lecture or Presentation

Title

Instruction Learner

Type (e.g. lecture, grand

rounds, formal

presentation)

Hours (actual

instruction)

Number of

Learners

Type of Learner(s)

(e.g. medical students, graduate students, residents)

2003 Physiology 204

Concepts of Biophysics

Lecture 6 8 Graduate Students

2005 - 2009

IGPBS Module 1

Lecture 10-14 23-27 Graduate Students

2006 - present

Protein Structure-Function

BCHM 923

Lecture 12 3 Graduate Students

2012 - present

Methods for Analyzing Biomolecules

Lecture 12 5 Graduate Students

198

BCHM 808

2012 - present

Biochemistry Res-Lit Seminar

BCHM 862

Seminar 15 8 Graduate Students

Nondidactic: e.g. workshops, labs, and discussion groups

Academic Year

Title and Course

Number

Presentation

Title

Instruction Learner

Type (e.g. small

group, laboratory,

seminar, workshop,

journal club)

Hours (actual

instruction)

Number of

Learners

Type of Learner(s)

(e.g. medical students, graduate students, residents)

2005 BCHM 801 Medical Biochemistry

Small group discussions

8 7 Medical Students

2006 BCHM 802 Medical Biochemistry

Small group discussions

10 7 Medical Students

2006 - present

Foundations of Medicine

Medical Biochemistry

Small group discussions

16 10 Medical Students

2006 - present

Genetics & Neoplasia

Medical Biochemistry

Small group discussions

4 10 Medical Students

Clinical:

Academic Year

Instruction Learner Length of Service (e.g. 8 weeks x 6 clerkships; one month

per resident; 40

hours/week x 6 weeks)

Type (e.g. clerkship, rounding,

clinic, daily supervision,

teaching) Hours Number of Learners

Type of Learner

(e.g. medical students, residents, fellows)

199

Master’s Theses and PhD Dissertations Directed: List only those for which you are/were primarily responsible.

Year Student Name Thesis Title

Degree

(completed/in progress)

2010 – present

2012 – present

Mauricio Vargas-Uribe

Cassandra Field

TBD

TBD

In progress

In progress

Supervision of Postdoctoral Fellows: List only those for which you are/were primary supervisor.

Year Fellow Name Area of Study

2004 - 2006

2004 - 2008

2005 - 2006

2006 - present

2007 - present

2012 - present

Sergiy S. Palchevckyy

Yevgen O. Posokhov

Somes K. Das

Mykola V. Rodnin

Alexander Kyrychenko

Chiranjib Ghatak

Biochemistry

Biochemistry

Biochemistry

Biochemistry

Biochemistry

Biochemistry

Advising: Thesis or dissertation committees; student academic group/individual

Date Student or Group Name Type of Student/Group

2006 - 2012

2009 – present 2005 – 2011

Hiroo Katayama

Ozan Kumru

Daniel Parente

Subhaschandra Naik

Damien Pechak, Anna Thoma & Joshua Brettmann

PhD thesis committee

Ph.D. thesis committee

Ph.D. thesis committee (Swint-Kruse lab)

Ph.D. thesis committee (M. Fisher lab)

Summer students from William Jewell College

Other Teaching Activities: CME and Faculty Department Seminars

Date Title of

Presentation Location Teaching Function

Type of Learner

200

2. Development of Educational Materials:

Describe any process of peer-review or expert assessment to which materials have been subjected. Specifically document if the materials have been used or referenced by colleagues, especially at other institutions or otherwise disseminated. Examples of course materials include syllabi, educational software packages, web sites, films, educational tapes, and evaluation tools.

Year Title Description Intended Audience

2006 - present Properties of Amino Acids

(eLearning web-based activity)

Medical Students

3. Educational Leadership: Describe administrative responsibility for courses and other leadership activities, including formal mentoring of junior faculty. Please list faculty members mentored and your role as mentor- letters from mentorees may be included.

I am a Course Director for BCHM 808 “Methods for Analyzing Biomolecules” and BCHM 862 “Literature Seminar”

III. SERVICE ACTIVITIES: Instructions available online.

See Guidelines for Promotion and Tenure, available online, for definitions and suggested documentation of professional and academic service. 1. Professional Service (Patient Care):

The diverse area of Professional Service includes patient care. Select measures that most clearly and concisely document your accomplishments and the value of these activities to the School of Medicine and the University. Measure of both quantity and quality of activities are required. If necessary, applicants should provide brief descriptions to assist reviewers. Measures of patient care activities include: number of patients, time allocation in clinical activity, procedures completed, Relative Value Units (RVUs), and value to the School of clinical service. If the primary quality evaluation is the subjective assessment of peers, this should be demonstrated in letters from departmental colleagues, chair, or referees. The significance of professional service in the forms of task forces, committees (e.g. University of Kansas Hospital and University of Kansas Physicians) and similar groups should be explained and your specific role clarified. Professional consulting services must have academic credibility and clear service intent and not be performed primarily for personal profit.

201

2. Academic Service: In Academic Service, your contribution to the academic community should be clearly documented. Names and dates of committees, task forces, or working groups should be provided. A brief description of the significance of the group and explanation your role should be provided. Activities related to University of Kansas School of Medicine Academic Societies should be documented in this section. Editorial Board Member: Journal of Membrane Biology Biopolymers and Cell Journal, Ukraine External grant reviewer for: Centre National de la Recherche Scientifique, CNRS, France Agence Nationale de la Recherche, ANR, France Natural Sciences and Engineering Research Council of Canada

Reviewer for the following journals: Biochemistry, Biophysical Journal, JMB, BBA, Biopolymers and Cell, Analytical Biochemistry, PNAS, Nature Communications, JBC, JACS, Langmuir, Physical Chemistry, J. of Pharmacology Reviewed advanced chapters of the book “Structural Principles of Membrane Protein Function by Englemann, White and von Hanije for Garland Science Publishing Reviewer for the KUMC Biomedical Research Training Program In charge of Departmental Research Retreat since 2006 Organizer of Telluride Research Workshop “Membrane Protein Folding and Functioning”, Telluride CO, August 5-9. 2013 Co-Chair of Platform Sessions on Protein-Lipid Interactions at Biophysical Society Meetings in New Orleans, LA, 2000, Baltimore, MD 2007, and San Francisco, CA, 2010 Co-Chair of Platform Sessions on Membrane Proteins at Biophysical Society Meetings in Salt Lake City, UT, 2006 and Long Beach, CA 2008

202

Session Chair and Discussion Leader for Lipid and Lipid Protein Interactions, Gordon Conference on Protons and Membrane Reactions, Ventura, CA, 2012. Session Chair, American Chemical Society Symposium on “Interfacially Actrive Peptides, New Orleans, LA, 2013.

IV. RESEARCH AND SCHOLARLY ACTIVITIES: Instructions available online. Opening Statement: Provide a brief opening statement to orient the reviewers to the principal areas of scholarly activity and provide your perspective. A description of your most significant contributions to the literature is often helpful to the reviewers. My research is devoted to understanding of physical principles of protein-membrane interactions and of the nature of conformational switching during physiological functioning, such as cellular entry by bacterial toxins and host-defence peptides, and lately, the regulation of apoptosis. The highlights of my accomplishments are devided into the following three categories: A. Protein-Membrane Interactions: Physical Principles. My past and present research has been aimed at deciphering the physical principles that determine the structure, assembly and functioning of membrane proteins. Specifically, we explored the formative interactions of membrane interfaces on protein structure. Our main accomplishments are: (1) quantitative characterization of the interplay of electrostatic and hydrophobic interactions on membrane interfaces, (2) quantitative characterization of the energetics of secondary structure formation on membranes, (3) introduction and development of the interface-directed membrane insertion concept for non-constitutive proteins, (4) determination of mechanism of action of antimicrobial and other host-defense peptides. Recently, in collaboration with Jean-Luc Popot (CNRS, Paris, France), we have developed a number of approaches for measurements of membrane protein folding and stability, based on application of novel fluorinated surfactants and amphipols. By combining FCS-based partitioning measurements and a novel protocol for chaperoned insertion, we have determined the free energy for bilayer insertion of bacterial toxin and that of a single transmembrane helix and calculated the free energy of transfer of helical backbone into the hydrocarbon core of the lipid bilayer. These are the first thermodynamically sound measurement of this kind which establish a thermodynamic scale of the molecular processes involved in membrane insertion/refolding transitions. B. pH-Triggered Conformational Switching and Membrane Insertion of Diphtheria Toxin T-Domain. In the past several years the works coming from our lab had made a breakthrough in understanding the mechanism of conformational switching between soluble and membrane-inserted forms of Diphtheria Toxin T-Domain (DTT) achived by

203

protonation. We have identified several insertion/refolding intermediates of DTT and determined free energy differences and transition rates for the transitions along the refolding/insertion pathway. We have identified a critical histidine residue, H257, involved in conformational switching of the T-domain in solution and demonstrated that C-terminal histidines (especially 322) are critical for the later stages of translocation. Our most recent work combines experiment and molecular dynamics simulations to reveal the molecular mechanism behind the conformational switch in DTT. The importance of these findings are not limited to the field of bacterial action, but is also related to our understanding of similar proteins, such as those of the BCL-2 family, regulating apoptosis. The initial characterization of conformational switching during membrane insertion of the pro-survival BCL-XL protein is on currently on the way in my lab. C. Development of Spectroscopic Methods for Studies of Membrane Proteins. Over the years we have developed of a series of spectroscopic techniques for following structural dynamics of macromolecules and for structural and thermodynamic studies of membrane proteins and peptides. We have developed or advanced the following spectroscopic techniques utilized to obtain the results presented above: distribution analysis methodology for depth-dependent fluorescence quenching analysis of membrane penetration; the lifetime-based membrane topology method, quenching-enhanced titration method for membrane binding measurements; lifetime/steady-state FRET protocol for protein oligomerization on membranes; fluorescence lifetime quenching with transition ion metals as short-range conformational probes; chaperoned insertion protocol by fluorinated surfactants for thermodynamic analysis of stability and insertion and an FCS-based protocol for thermodynamic analysis of pH-dependent membrane interactions. Our lab has also participated in the development of a high-performance time-resolved fluorescence spectrometer. Currently we are developing a concerted approach for structural characterization of membrane proteins using fluorescence spectroscopy and Molecular Dynamics simulations.

1. Grants and Contracts:

Information must include whether you are the principal investigator or a co-investigator, names of all investigators, title of grant, funding source, dollar amount in direct costs, and years during which grant applies. Co-investigators must specify role. Provide the cover sheet, abstract, and Notice of Award in PDF for all grants or contracts awarded in last five years.

Previous Grants and Contracts Awarded: List in chronological order; oldest first, newest last; list the applicant’s name in BOLD.

Principal Investigator Investigators

Title of Grant/Contract

Funding Source

Direct Costs Inclusive

Years of Status

204

Award

Narayan Ladokin, A. Effect of Hemifluorinated Surfactants on Membrane Insertion/Folding of Diphtheria Toxin T-Domain

NIH/NCRRR 100,000

2004-2006

Completed

Ladokhin 5RO1GM069783 Competing supplement to: pH-Triggered Membrane Insertion of Proteins

NIH $422,228 2007-2009

Completed

Ladokhin 3RO1GM069783-05S1 ARRA administrative supplement to: pH-Triggered Membrane Insertion of Proteins

NIH $62,699 2009-2010

Completed

Ladokhin KUMC Research Institute bridging grant

KUMC $30,000 2010-2011

Completed

Current Grants and Contracts Awarded: List in chronological order; oldest first, newest last; list the applicant’s name in BOLD.

Principal Investigator Investigators

Title of Grant/Contract

Funding Source

Direct Costs

Inclusive Years of Award Status

Ladokhin Tobias, Kurnikova, Cocco

pH-Triggered Membrane Insertion of Proteins

NIH 988,000 2011-2015

Funded

Grants and Contracts Submitted: List in chronological order; oldest first, newest last; list the applicant’s name in BOLD.

Principal Investigators

Title of Funding Direct Inclusive Status

Comment [AL1]: Wendy, I need your help completing this column.

205

Investigator Grant/Contract Source Costs Years of

Award

Goldberg Ladokhin, Ritter, Khattree, Dang

Molecular scaffolding in photoreceptor renewal and retinal disease

NIH 1,250,000 2013 – 2018

Pending Review

2. Scholarly Publications:

Publications must be numbered in chronological order, oldest publication first, most recent publications last. In cases where there are multiple publications within a year, they should be listed in alphabetical order.

Articles published: Include full-length, peer-reviewed articles in scientific journals; this list should not include papers in preparation, submitted, or under revisions, nor should it include conference proceedings, published abstracts, and book reviews (which are listed separately). Provide names of all authors (applicant’s name in BOLD), year, title, journal, volume, and inclusive pages. Provide a PDF of each peer-review article published within the last five years. Other articles may be provided at the applicant’s discretion.

1. Demchenko, A. P., Ladokhin, A. S., Kostrzevska, E. G. & Dibrova, T. L., 1987, Structural dynamics in the environment of the tryptophan residue in melittin. Molecular Biology 21, 553 560.

2. Kamalov, V. F., Ladokhin, A. S. & Toleutaev, B. N., 1987, Intramolecular nanosecond dynamics of melittin. Proc. Acad. Sci. USSR 296, 742 745.

3. Demchenko, A. P. & Ladokhin, A. S., 1988, Red-edge excitation fluorescence spectroscopy of indole and tryptophan. Eur. Biophys. J. 15, 369 379.

4. Demchenko A. P. & Ladokhin, A. S., 1988, Temperature-dependent shift of fluorescence spectra without conformational changes in protein: Studies of dipole relaxation in the melittin molecule. Biochim. et Biophys. Acta. 955, 352 360

5. Ladokhin, A.S., Kostrzevska, E.G. & Demchenko, A.P., 1988, Interaction of melittin with phospholipid bilayer, Proc. Acad. Sci Ukraine, 11(C), 65-58

6. Hershman, P., Ladokhin, A.S., Lebedeva, N.V. & Chikishev, A. Yu, 1989, Nanosecond dynamics of protein: Time-resolved fluorescent spectroscopy. Bull. Moscow State University, Ser. 3 30, 47-52.

7. Ladokhin, A. S., Lebedeva, N.V. & Chikishev, A. Uy., 1989, Study of melittin membrane complex by time-resolved fluorescent spectroscopy. Biopolymers and Cell, 5, 100-102.

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8. Chikishev, A. Yu., Ladokhin, A.S., Lebedeva, N.B. & Toleutaev, B.N., 1990,

Picosecond fluorescence spectrochronography of protein melittin and melittin-membrane complexes. J. of Molecular Structure, 291, 347-352.

9. Ladokhin, A.S., 1990, Equilibrium dynamics in protein. Melittin intrinsic fluorescence study. Biopolymers and Cell, 6, 84-90.

10. Ladokhin, A. S., Wang, L., Steggles, A. W. & Holloway, P. W., 1991, Fluorescence study of a mutant cytochrome b5 with a single tryptophan in the membrane binding domain. Biochemistry 30, 10200–10206.

11. Ladokhin, A. S., Holloway, P. W. & Kostrzhevska, E. G., 1993, Distribution analysis of membrane penetration by depth-dependent fluorescence quenching. J. of Fluorescence 3, 195–197.

12. Ladokhin, A. S., Wang, L., Steggles, A. W., Malak, H. & Holloway, P. W., 1993, Fluorescence study of a temperature induced conversion from the “loose” to the “tight” binding form of membrane-bound cytochrome b5. Biochemistry 32, 6951–6956.

13. Tretyachenko-Ladokhina, V. G., Ladokhin, A. S., Wang, L., Steggles, A. W. & Holloway, P. W., 1993, Amino acid substitutions in the membrane-binding domain of cytochrome b5 alter its membrane-binding properties. Biochim. et Biophys. Acta 1153, 163–169.

14. Ladokhin, A. S. & Brand, L., 1995, Evidence for an excited-state reaction contributing to NADH fluorescence. J. of Fluorescence 5, 99–106.

15. Ladokhin, A. S. & Holloway, P. W., 1995, Fluorescence of membrane-bound tryptophan octyl ester. A model for studying intrinsic fluorescence of protein–membrane interactions. Biophys. J. 69, 506–517.

16. Ladokhin, A. S. & Holloway, P. W., 1995, Fluorescence quenching study of melittin-membrane interactions. Ukrainian Biochem. J. 67, 34–40.

17. Ladokhin, A. S., Wimley, W. C. & White, S. H., 1995, Leakage of membrane vesicle contents: Determination of mechanism using fluorescence requenching. Biophys. J. 69, 1964–1971.

18. Ladokhin, A. S., Selsted, M. E. & White, S. H., 1997, Bilayer interactions of indolicidin, a small antimicrobial peptide rich in tryptophan, proline, and basic amino acids. Biophys. J. 72, 794–805.

19. Ladokhin, A. S., Selsted, M. E. & White, S. H., 1997, Sizing membrane pores in lipid vesicles by leakage of co-encapsulated markers: Pore formation by melittin. Biophys. J. 72, 1762–1766.

20. Petrushenko, Z. M., Negrutskii, B. S., Ladokhin, A. S., Budkevich, T. V., Shalak, V. F. & El’skaya, A. V., 1997, Evidence for the formation of an unusual ternary complex of rabbit liver EF-1 with GDP and deacylated

tRNA. FEBS Letters 407, 13–17. 21. Wimley, W. C., Hristova, K., Ladokhin, A. S., Silvestro, L., Axelsen, P. H. &

White, S. H., 1998, Folding of -sheet membrane proteins: A hydrophobic hexapeptide model. J. Mol. Biol. 277, 1091–1110 .

207

22. Ladokhin, A. S., 1999, Analysis of protein and peptide penetration into

membranes by depth-dependent fluorescence quenching: Theoretical considerations. Biophys. J. 76, 946–955.

23. Ladokhin, A. S., 1999, Evaluation of lipid exposure of tryptophan residues in membrane peptides and proteins. Analytical Biochem. 276, 65–71.

24. Ladokhin, A. S., 1999, Red-edge excitation study of non-exponential fluorescence decay of indole in solution and in a protein. J. of Fluorescence 9, 1–10.

25. Ladokhin, A. S., 1999, Selsted, M. E. & White, S. H. CD spectra of indolicidin antimicrobial peptides suggest turns, not polyproline helix. Biochemistry 38, 12313–12319.

26. Ladokhin, A. S. & White, S. H., 1999, Folding of amphipathic -helices on membranes: Energetics of helix formation by melittin. J. Mol. Biol. 285, 1363–1369.

27. Ladokhin, A. S., Jayasinghe, S. & White, S. H., 2000, How to measure and analyze tryptophan fluorescence in membranes properly, and why bother? Analytical Biochem. 285, 235–245.

28. Osapay, K., Tran, D., Ladokhin, A. S., White, S. H., Henschen, A. H. & Selsted, M. E., 2000, Formation and characterization of a single Trp-Trp crosslink in indolicidin that confers protease stability without altering antimicrobial activity. J. Biol. Chem. 275, 12017–12022.

29. Ladokhin, A. S., 2001, On the interpretation of decay-associated fluorescence spectra in proteins. Biopolymers & Cell 17, 221–224.

30. Ladokhin, A. S. & White, S. H., 2001, Alphas and taus of tryptophan fluorescence in membranes. Biophys. J. 81, 99055–99058.

31. Ladokhin, A. S. & White, S. H., 2001, ‘Detergent-like’ permeabilization of anionic lipid vesicles by melittin. Biochim. et Biophys. Acta, 1514, 253–260.

32. Ladokhin, A. S. & White, S. H., 2001, Protein chemistry at membrane interfaces: Non-additivity of electrostatic and hydrophobic interactions. J. Mol. Biol. 309, 543–552.

33. Ladokhin, A. S., Isas, J. M., Haigler, H. T. & White, S. H., 2002, Determining the membrane topology of proteins: Insertion pathway of a transmembrane helix of annexin 12. Biochemistry 41, 13617-13626.

34. Ladokhin, A. S., Legmann, R., Collier, R. J. & White, S. H., 2004, Reversible refolding of the diphtheria toxin T-domain on lipid membranes. Biochemistry 43, 7451-7458.

35. Ladokhin, A. S. & White, S. H., 2004, Interfacial folding and membrane insertion of a designed helical peptide. Biochemistry 43, 5782-5791.

36. Ladokhin, A. S. and Haigler, H. T., 2005, Reversible Transition between the Surface Trimer and Membrane-Inserted Monomer of Annexin 12. Biochemistry 44, 34042-3409.

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37. Patel, D. R., Isas, J. M., Ladokhin, A. S., Jao, C. C., Kim, Y. E., Kirsch, T.,

Langen. R. and Haigler, H. T., 2005, The Conserved Core Domains of Annexins A1, A2, A5, and B12 Can Be Divided into Two Groups with Different Ca2+ -Dependent Membrane-Binding Properties. Biochemistry 44, 2833-2844.

38. Palchevskyy, S.S, Posokhov, Y.O., Olivier, B., Popot, J-L, Pucci, B. and Ladokhin, A. S., 2006 Chaperoning of insertion of membrane proteins into lipid bilayers by hemifluorinated surfactants: application to diphtheria toxin Biochemistry 45, 2629-2635.

39. Posokhov, Y.O. and Ladokhin, A. S., 2006, Lifetime Fluorescence Method for Determining Membrane Topology of Proteins, Analytical Biochem. 348, 87-93.

40. Fernandez-Vidal M., Jayasinghe S., Ladokhin A.S., White S.H., 2007, Folding Amphipathic Helices Into Membranes: Amphiphilicity Trumps Hydrophobicity, J. Mol. Biol. 370(3):459-70.

41. Posokhov, Y.O., Gottlieb, P.A. and Ladokhin, A. S., 2007, Quenching-enhanced fluorescence titration protocol for accurate determination of free energy of membrane binding, Analytical Biochem. 362, 290-292.

42. Posokhov, Y.O., Gottlieb, P.A., Morales, M.J., Sachs, F. and Ladokhin, A. S. ,2007, Is Lipid Bilayer Binding a Common Property of Inhibitor Cysteine Knot Ion-Channel Blockers? Biophysical Journal 93(4):L20-2.

43. Ladokhin, A. S., 2008, Insertion Intermediate of Annexin B12 is Prone to Aggregation on Membrane Interfaces. Biopolymers and Cell, 24(2):100-104.

44. Posokhov, Y.O., Merzlyakov, M., Hristova, K. and Ladokhin, A. S., 2008, A simple "proximity" correction for Förster resonance energy transfer efficiency determination in membranes using lifetime measurements, Analytical Biochem., 380, 134-136.

45. Posokhov, Y.O., Rodnin M.V., Das S.K., Pucci B., Ladokhin A.S., 2008, FCS study of the thermodynamics of membrane protein insertion into the lipid bilayer chaperoned by fluorinated surfactants. Biophysical Journal, 95, L54-6..

46. Posokhov, Y.O., Rodnin, M.V., Lu, L. and Ladokhin, A. S., 2008, Membrane Insertion Pathway of Annexin B12: Thermodynamic and Kinetic Characterization by FCS and Fluorescence Quenching. Biochemistry 47, 5078-87.

47. Rodnin, M.V., Posokhov, Y.O., Contino-Pepin, C., Brettmann, J., Kyrychenko, A., Palchevskyy, S.S.. Pucci, B., and Ladokhin, A. S., 2008, Interactions of fluorinated surfactants with diphtheria toxin T-domain: Testing new media for studies of membrane proteins. Biophysical Journal, 94, 4348-57.

48. Kyrychenko, A., Posokhov, Y.O., Rodnin M.V., Ladokhin A.S., 2009, Kinetic intermediate reveals staggered pH-dependent transitions along

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the membrane insertion pathway of the diphtheria toxin T-domain. Biochemistry, 48, 7584-94.

49. Kyrychenko A., Wu F., Thummel R.P., Waluk J., Ladokhin A.S., 2010, Partitioning and Localization of Environment-Sensitive 2-(2′-Pyridyl)- and 2-(2′-Pyrimidyl)-Indoles in Lipid Membranes: A Joint Refinement Using Fluorescence Measurements and Molecular Dynamics Simulations J. Phys. Chem. B 114, 13574-13584.

50. Ladokhin A.S., Fernandez-Vidal M., White S.H., 2010, CD Spectroscopy of Peptides and Proteins Bound to Large Unilamellar Vesicles, J. Membrane Biol. 236, 247-253.

51. Muretta J.M., Kyrychenko A., Ladokhin A.S., Kast D.J., Gillispie G.D., and Thomas D.D., 2010, High-performance time-resolved fluorescence by direct waveform recording, Rev. Sci. Instrum. 81, 103101.

52. Posokhov, Y.O., Kyrychenko, A., Ladokhin A.S., 2010, Steady-state and time-resolved fluorescence quenching with transition metal ions as short-distance probes for protein conformation. Analytical Biochem., 407, 284-286.

53. Rodnin M.V., Kyrychenko A., Kienker P., Sharma O., Posokhov Y.O., Collier R.J., Finkelstein A., Ladokhin A.S., 2010, Conformational Switching of the Diphtheria Toxin T Domain. J. Mol. Biol, 402, 1-7.

54. Fernandez-Vidal M., White S.H., Ladokhin A.S., 2011, Membrane partitioning: "classical" and "nonclassical" hydrophobic effects. J. Membrane Biol., 239, 5-14.

55. Kyrychenko A., Sevriukov I.Y, Syzova Z.A, Ladokhin A.S., 2011, Doroshenko A.O. Partitioning of 2,6-Bis(1H-Benzimidazol-2-yl)pyridine fluorophore into a phospholipid bilayer: Complementary use of fluorescence quenching studies and molecular dynamics simulations. Biophys. Chem, 154, 8-17.

56. Kumru OS, Schulze RJ, Rodnin MV, Ladokhin A.S., Zückert W.R., 2011, Surface localization determinants of Borrelia OspC/Vsp family lipoproteins J. Bacteriol. 193:2814-25.

57. Rodnin M.V., Kyrychenko A., Kienker P., Sharma O., Vargas-Uribe M., Collier R.J., Finkelstein A., Ladokhin A.S. Replacement of C-Terminal Histidines Uncouples Membrane Insertion and Translocation in Diphtheria Toxin T-Domain. Biophysical Journal, 101:L41-L43.

58. Almeida P., Ladokhin A.S., 2012, White S.H. Hydrogen-bond energetics drive helix formation in membrane interfaces. BBA, Special Issue “Membrane protein structure and function”, 1818:178-182.

59. Kyrychenko A., Rodnin M.V., Posokhov Y.O., Halt A., Pucci B., Killian J.A., Ladokhin A.S., 2012, Thermodynamic measurements of bilayer insertion of a single transmembrane helix chaperonned by fluorinated surfactants. J. Mol. Biol, 416:328-334.

60. Kyrychenko A., Rodnin M.V., Vargas-Uribe M., Sharma S.K., Durand G., Pucci B., Popot J.-L., Ladokhin A.S., 2012, Folding of diphtheria toxin T-

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domain in the presence of amphipols and fluorinated surfactants: Toward Thermodynamic measurements of membrane protein folding. BBA, Special Issue “Protein folding in membranes”, 1818:1006-12.

61. Kyrychenko A., Tobias D.J., Ladokhin A.S., 2013, Validation of Depth-Dependent Fluorescence Quenching in Membranes by Molecular Dynamics Simulation of Tryptophan Octyl Ester in POPC Bilayer. J. Phys. Chem. B., 117, 4770-8.

Manuscripts in press: Provide names of all authors (applicant’s name in BOLD), title, journal, and PDF of manuscript, plus evidence of acceptance by journal. 1. Kurnikov I., Kyrychenko A., Flores-Canales J., Rodnin M.V., Simakov N.,

Vargas-Uribe M., Posokhov Y.O., Kurnikova M., Ladokhin A.S. pH-Triggered conformational switching of the diphtheria toxin T-domain: The roles of N-terminal histidines. JMB.

2. Kyrychenko A., Ladokhin A.S. Molecular Dynamics Simulation of Depth Distribution of Spin-Labeled Phospholipids within Lipid Bilayer. J. Phys. Chem. B.

3. Vargas-Uribe M., Rodnin M.V., Kienker P., Finkelstein A., Ladokhin A.S. Crucial Role of H322 in the Folding of Diphtheria Toxin T-Domain into the Open-Channel State. Biochemistry.

Manuscripts submitted by not yet accepted: Provide names of all authors (applicant’s name in BOLD), title, journal, and PDF of manuscript, plus evidence of acceptance by journal. 1.

Invited or non-peer –reviewed articles or reviews: Provide names of all authors (applicant’s name in BOLD), year, title, journal, volume, and pages. If in press, provide documentation and PDF of article/review if published within the last five years.

1. Ladokhin, A. S., 1997, Distribution analysis of depth-dependent fluorescence quenching in membranes: A practical guide. Methods in Enzymology 278, 462–473.

2. Ladokhin, A. S., Wimley, W. C., Hristova, K., & White, S. H., 1997, Mechanism of leakage of contents of membrane vesicles determined by fluorescence requenching. Methods in Enzymology 278, 474–486.

3. White, S. H., Wimley, W. C., Ladokhin, A. S, & Hristova, K., 1998, Methods for determining the energetics of peptide–bilayer interactions. Methods in Enzymology 295, 62–87.

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4. White, S. H., Wimley, W. C., Ladokhin, A. S, & Hristova, K., 1998, Protein

folding in membranes: Pondering the nature of the bilayer milieu. Biol. Skr. Dan. Selsk. 49, 99–106.

5. White, S. H., Ladokhin, A. S, Jayasinghe, S. & Hristova, K., 2001, How membranes shape protein structure. J. Biol. Chem. 276, 32395–32398.

6. Ladokhin, A. S., 2009, Fluorescence spectroscopy in thermodynamic and kinetic analysis of pH-dependent membrane protein insertion. Methods in Enzymology 466, 19-42.

Books and book chapters: Provide names of all authors (applicant’s name in BOLD), year, book title, chapter title, edition, publisher, and pages. If in press, provide documentation and PDF of book/book chapter if published in last five years.

1. Ladokhin, A. S., 2000, Encyclopedia of Analytical Chemistry: Instrumentation and Applications, “Fluorescence spectroscopy in peptide and protein analysis”, 15 volume set editions, Wiley, 5762-5779.

2. London, E. & Ladokhin, A. S., 2002, Current Topics in Membranes: Peptide–Lipid Interactions, “Measuring the depth of amino acid residues in membrane-inserted peptides by fluorescence quenching”, 1 edition, Academic Press, 89-115.

Published Abstracts: Provide names of all authors (applicant’s name in BOLD), year, title, where published, volume and pages. If the same work is reflected in a published abstract and a presentation or poster, the work must only be listed once. 1.

Other Scholarly Publications: 1.

3. Presentations and Posters:

Presentations and posters must be number in chronological order, oldest first, most recent last.

Oral paper presentation: Provide names of all authors (applicant’s name in BOLD), title, sponsoring organization, extent of peer-review, and location and date of presentation.

Scientific papers presented at national and international meetings:

212

1. Ladokhin, A.S., ‘Fluorescence approaches for studying membrane

structure and dynamics.’ Biophysical Society Meeting, Baltimore, MD, 1999. Fluorescence Subgroup Panel Discussion

2. Ladokhin, A.S., Biophysical Society Meeting, New Orleans, LA (2000). Co-Chair of Platform Session on Protein–Lipid Interactions.

3. Ladokhin, A.S., “Reversible Refolding of the Diphtheria Toxin T-domain on Membranes”ASBMB Meeting, San Diego, CA, 2003

4. Ladokhin, A.S., Gordon Conference on Antimicrobial Peptides, Ventura, CA, 2005.

5. Ladokhin, A.S., American Chemical Society Meeting, San-Francisco, CA, 2006. , speaker

6. Ladokhin, A.S., Biophysical Society Meeting, Salt Lake City, UA, 2006. Co-Chair of Platform Session on Membrane Proteins.

7. Ladokhin, A.S., American Chemical Society Meeting, Atlanta, GA, 2006, speaker

8. Ladokhin, A.S., “pH-triggered membrane protein folding and insertion: Annexin B12 as a model” VI International Annexin Conference, Avalon, CA, 2007. Invited Speaker

9. Ladokhin, A.S., “Using novel fluorinated surfactants to chaperone membrane protein insertion” NIH/NIAAA/Lab. Mem. Biochem & Biophys., Bethesda, MD, 2007. (Lecture)

10. Ladokhin, A.S., Biophysical Society Meeting, Baltimore, MD, 2007. Co-Chair of Platform Session on Lipid-Protein Interactions.

11. Ladokhin, A.S., Biophysical Society Meeting, Long Beach, CA, 2008. Co-Chair of Platform Session on Membrane Proteins.

12. Ladokhin, A.S., 23rd Annual Gibbs Conference in Biothermodynamics, Carbondale, IL, 2009, invited speaker

13. Ladokhin, A.S., Gordon Conference on Biophysics of Membrane Transport, Waterville, ME, 2009, invited speaker

14. Ladokhin, A.S., Workshop on Biophysics of Ion Channels, Telluride, CO, 2009, invited speaker

15. Ladokhin, A.S., “Frontiers in Membrane and Membrane Protein Biophysics: Experiment and Theory” Symposium, Irvine, CA, 2010, speaker

16. Ladokhin, A.S., FASEB Meeting on Biophysics of Cellular Membrane, Saxtons River, Vermont 2010. Oral presentation selected from the abstracts

17. Ladokhin, A.S., Biophysical Society Meeting, San Francisco, CA, 2010. Co-Chair of Platform Session on Lipid-Protein Interactions.

18. Ladokhin, A.S., Biophysical Society Meeting, Baltimore, MD, 2011. Membrane Structure and Assembly Subgroup Symposium, Invited Speaker.

213

19. Ladokhin, A.S., “Conformational switching during pH-triggered

membrane protein insertion” FASEB Meeting on Biophysics of Cellular Membrane, Snowmass, CO, 2012

20. Ladokhin, A.S., Gordon Conference on Protons and Membrane Reactions, Ventura, CA, 2012, Session Chair and Discussion Leader for Lipid and Lipid Protein Interactions

21. Ladokhin, A.S., Membrane Protein Folding Symposium of the Biophysical Society, Seoul, South Korea, 2013

22. Ladokhin, A.S., “Interfacially Active Peptides”, American Chemical Society Symposium on New Orleans, LA 2013, Session Chair and invited speaker

Scientific papers presented at local and regional meetings: 1.

Poster Presentations: Provide names of all authors (applicant’s name in BOLD), title, sponsoring organization, extent of peer-review, and location and date of presentation.

Poster presentation at national and international meetings: 1. Poster presentation at local and regional meetings: 1.

Invited seminars at other universities: Provide title, sponsoring organization or institution, and date of presentation.

1. “Excited-state reactions and NADH fluorescence”, Agricultural University, Wageningen, The Netherlands, 1993

2. “Leakage of membrane vesicle contents: Determination of mechanism using fluorescence requenching”, Beckman Laser Institute, Irvine, CA, 1995

3. “Membrane permeabilization: Pieces of the puzzle”, Rutgers Univ., New Brunswick, NJ, 1998

4. “Membrane protein folding: Thermodynamic and kinetic control”, Institute of Molecular Biology and Genetics, Kiev, Ukraine, 2001

5. Lecture, SNRS, Institut de Biologie Physico-Chimique, Paris, 2005 6. “Membrane Protein Folding: from Basic Principles to Insertion

Pathways”, UMKC, Kansas City, MO, 2005

214

7. “Membrane Protein Folding: from Basic Principles to Insertion

Pathways”, University of Aalborg, Department of Life Sciences, Aalborg, Denmark, 2006

8. “Membrane Protein Folding: from Basic Principles to Chaperoned Insertion”, Tulane University, New Orleans, LA, 2007

9. “Why study single biomolecules?”, Institute of Molecular Biology and Genetics, Kiev, Ukraine, 2007

10. “pH-Triggered Membrane Protein Insertion: Thermodynamic and Kinetic Aspects”, Carnegie Mellon University, Pittsburgh, PA, 2009

11. “Thermodynamics of pH-Triggered Membrane Protein Insertion: what can we learn about membrane protein stability?” Boston University, Boston, MA, 2009

12. “Kinetic and thermodynamic studies of membrane insertion of diphtheris toxin T-domain “, SNRS, Institut de Biologie Physico-Chimique, Paris, France, 2010

13. “Fluorinated surfactants as chaperons for thermodynamic studies of membrane proteins“, Université de Avignon, Avignon, France, 2010

14. “Kinetic and thermodynamic studies of membrane insertion of diptheris toxin T-domain”, Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland, 2010

15. “Why study single biomolecules: One lab perspective”, Institute for Biotechnology, Shevchenko National University, Kiev, Ukraine, 2010

16. “pH-Triggered Conformational Switching and Membrane Insertion of Diphtheria Toxin T-Domain”, Department of Chemistry, UC-Irvine, 2013

17. “Deciphering pH-Triggered Membrane Protein Insertion with Experiment and Simulation”, Department of Chemistry, Washington Univ. St. Louis, 2013

18. “Deciphering pH-Triggered Membrane Protein Insertion with Experiment and Simulation”, Department of Chemistry, Univ. of Missouri-Columbia, 2013

4. Other Evidence of Scholarship:

Includes clinical guidelines, policy documents, contributions to significant position statements by professional organizations, and development of national examinations. 1.

VII. VIII. IX.

215

First Name Owen

Last name Nadeau

Current Academic Rank Research Assistant Professor

Department(s) Dept. of Biochemistry and Molecular Biology

Office Address 1061 Hemenway Building

Phone 913-588-3486

Fax 913-588-9896

Email onadeau@kumc.edu

PROFESSIONAL DEVELOPMENT

Undergraduate and Graduate Education

Years (Inclusive) Degree Institution

1977 – 1981 B.S., Chemistry Indiana University of Pennsylvania, Indiana, PA

1981-1984 M.S., Chemistry Indiana University of Pennsylvania, Indiana, PA

1984 – 1991 Ph.D., Biochemistry University of Vermont, Burlington, VT

Postgraduate Education

Years (Inclusive) Degree Institution

1991-1997 Postdoctoral Fellow (Biochemistry)

University of Tennessee, Memphis, TN

1997 Postdoctoral Fellow (Pathology)

University of Tennessee, Memphis, TN

Academic and Professional Appointments and Activities (List in chronological order. Please explain any discontinuity in professional experience)

Month and Year Position Institution

1982-1984 Teaching Assistant Indiana University of Pennsylvania, PA

216

1984-1988 Teaching Fellow (Chemistry) University of Vermont,

Burlington, VT

1988-1991 Research Fellow (Biochemistry

University of Vermont, Burlington, VT

1991 – 1997 Postdoctoral Fellow (Biochemistry and Molecular Biology)

University of Tennessee, Memphis, TN

1997-1999 Senior Scientist, Group Leader

Hexos Inc, Bothell, WA

1999-2002 Research Assistant Professor University of Missouri – Kansas City

20030- present Research Assistant Professor University of Kansas Medical Center

Professional Registration/Licensure

Year Number State

Professional Societies and Affiliations

Date Organization (including offices held)

1989-1995 Sigma Xi

1990- present Protein Society

2004 – present American Society for Mass Spectrometry

1995 – present American Society for Biochemistry and Molecular Biology

1982 - present American Chemical Society

Honors and Awards (honorary societies, research awards, teaching and other awards)

Year Award

1984 Academic Achievement Award, Indiana University of Pennsylvania

1984 Graduate Research Award, Indiana University of Pennsylvania

217

1993-1995 American Heart Associate, Tennessee Affiliate –

Postdoctoral Research Fellowship

au

X. TEACHING ACTIVITIES (Teaching evaluations and other evidence of quality teaching must be attached; this represents the teacher’s portfolio and should be accurately summarize ALL of your teaching activities.) Brief statement of areas of teaching interest:

7. Instruction:

Didactic (e.g.: lectures and formal presentations)

Academic Year Course Title

Instruction Student

Type Hours No Type

2004 BCHM 850

Protein-Protein Interactions

Lecture Graduate

2009 BCHM 923

Adv. Protein Structure and Function

Lecture 2 Graduate

2011 BCHM 923

Adv. Protein Structure and Function

Lecture 2 Graduate

Nondidactic (e.g.: workshops, labs, and discussion groups)

Academic Year Course Title

Instruction Student

Type Hours No Type

2006 Foundations Medicine (1st Yr SOM)

Facilitator Small Group Discussions

M1

2007 Foundations Medicine (1st Yr SOM)

Facilitator: Cancer Screening

Small Group Discussions

M1

218

2008 Genetics &

Neoplasia (1st Yr SOM)

Facilitator: Genetics/Neoplasia

Small Group Discussions

M1

2009 Foundations Medicine (1st Yr SOM)

Facilitator: Cancer Screening

Small Group Discussions

M1

2009 Foundations Medicine (1st Yr SOM)

Facilitator: Huntingtins

Small Group Discussions

M1

2010 Genetics & Neoplasia (1st Yr SOM)

Facilitator: Genetics/Neoplasia

Small Group Discussions

M1

Clinical

Year Hours

Student Length of Service

No Type

Master’s Theses and PhD Dissertations directed

Year Student Name Thesis Title Degree

(Completed/In process)

Supervision of Postdoctoral Fellows

Year Fellow Name Area of Study

Advising (Thesis or dissertation committees: student academic group/individual)

Date Student or group name Type of Student/group

Other teaching activities

219

Date Title Place Teaching Function

8. Development of Educational Materials (Course materials e.g. syllabi, educational software packages, web sites, films, educational tapes and evaluation tools)

Year Title Description Intended Audience

2007 Post-translational modification of Proteins Web resource for Medical

Curriculum

9. Educational Leadership (Responsibility for courses and other leadership activities including mentoring of junior faculty. Please list faculty members mentored – letters from mentorees may be provided.)

SERVICE ACTIVITIES See guidelines and instructions to applicants for definitions and suggested documentation of professional and academic service. Professional Service: The diverse area of professional service includes patient care. Applicants should select measures that most clearly and concisely document their accomplishments and the value of these activities to the Medical School and University. Measures of both quantity and quality of activities are required and if necessary, applicants should provide brief descriptions to assist reviewers. Measures of patient care activities include numbers of patients, time allocation in clinical activity, procedures completed, Relative Value Units (RVUs), and value to the School of the clinical service. If the primary quality evaluation is the subjective assessment of peers, this should be available in letters from departmental colleagues, chair, or referees. The significance of professional service in the forms of task forces, committees and similar groups should be explained and the specific role of the applicant clarified. Professional consulting services must have academic credibility and clear service intent and not be performed primarily for personal profit.

Academic Service: In academic service the contribution of the candidate to the academic community should be clearly documented. Names and dates of committees, task forces, or working groups should be provided. A concise description of the significance of the group and explanation of the role of the applicant should be provided. Activities related to Academic Societies should be documented in this section.

220

XI. RESEARCH and SCHOLARLY ACTIVITIES:

Brief statement of areas of research and scholarly interest, including current projects:

Our primary goal and continuing efforts are centered on determining how subunit interactions affect the activation state of phosphorylase kinase (PhK), a 1.3 MDa hexadecameric complex comprising four copies of four distinct subunits, termed α, β, γ and δ, which is endogenous calmodulin. In the PhK complex, the catalytic γ subunit is activated by neural (Ca2+), hormonal (cAMP and Ca2+) and metabolic (ADP) stimuli, which are integrated through allosteric sites on the regulatory α, β and δ subunits. This activation of PhK by diverse physiological signals allows for tight control of glycogenolysis. Activation of PhK by Ca2+ in skeletal muscle directly links muscle contraction with energy production in the cascade activation of glycogen utilization. To analyze the subunit interactions of PhK we have developed a data-based approach to detect protein-protein interactions using a combination of chemical cross-linking and mass spectrometric (MS) methodologies. Recent developments in MS technologies have significantly reduced the amount of starting material required to detect specific contact regions between interacting proteins, leading to a resurgence in classical protein chemical approaches, including chemical cross-linking. Despite the resolving power of MS, considerable computational analyses of digests from conjugates of interacting proteins are required to predict all the possible masses that can arise from monoderivatization, intramolecular cross-linking, incomplete digestion of the target proteins, hydrolysis and other side reactions associated with chemical cross-linking. Considering the complexity of the products formed, we have developed a search engine that predicts both conjugates and side-products of such reactions. The search engine automatically eliminates false positives arising from known competing side reactions, thus allowing for rapid screening of MS peptide maps of protein conjugates for potential cross-linked peptides, which are then ranked hierarchically by theoretical best match. Since several combinations of cross-linking can lead to identical mass assignments within the error limits of state-of-the-art MS technologies, all cross-linked peptides must be verified by fragmentation (MS/MS) analyses, which in turn generate even more complex forms of data output that often must be annotated by hand. To eliminate this ‘second’ rate limiting step in the method, we are currently expanding the search engine’s capability to generate potential fragment ion patterns for those cross-linked peptides that are predicted in the first round of the analysis. A web-based version of the engine is under construction, and the initial results can be viewed at http://funnybase.umkc.edu/prot_cross3/.

Grants and contracts (Information must include whether the nominee (name bolded) is the principal investigator or a co-investigator, names of all investigators, title of grant, funding source, dollar amount in direct costs, and years during which grant applies. Co-investigators must specify role). Provide the cover sheet, abstract and Notice of Award in PDF for all grants or contracts awarded in last five years (submit online).

8. Previous Grants and contracts awarded: (List in chronological order – oldest first, newest last)

221

Principal

Investigator Investigators Title of Grant

Funding Source

Direct Costs Years Status

Current Grants and contracts awarded: (List in chronological order – oldest first, newest last)

Principal Investigator Investigators

Title of Grant

Funding Source

Direct Costs Years Status

Grants and contracts submitted:

Principal Investigator Investigators Title of Grant

Funding Source

Direct Costs Years Status

Owen Nadeau and

Xiuxia Du

XlinkID: An Automated Data Analysis Platform for Structural Studies of Proteins Using Chemical Cross-Linking and

Tandem Mass Spectrometry NIH 123,000 1-4 pending

9. Scholarly Publications

Full length, peer-reviewed articles in scientific journals: (Provide names of all authors, year, title, journal, volume, and inclusive pages. The articles must be numbered in chronological order (oldest publications first, most recent publications last). In cases where there are multiple publications within a year, they should be listed by alphabetical order. The list should not include papers "in preparation," "submitted," or "under revisions", nor should it include conference proceedings, published abstracts, and book reviews (which need to be listed separately). Articles published: (Provide a PDF of each peer-reviewed article published within the last five years. Other articles may be provided at the applicant's discretion.)

1. Hubbard, J.L., Morneau, A., Burns, R.M. and Nadeau, O.W. (1991) Carbon-carbon Double Bond Formation from a cis-bis (chloromethyl) Complex, J. Am. Chem. Soc. 113:9180-9184.

2. Nadeau, O.W., Gump, D.W., Hendricks, G.M. and Meyer, D.H. (1992) Deposition of

Bismuth by Yersinia nterocolitica, Med. Microbiol. Immunol. 181:145-152.

222

3. Gump, D.W., Nadeau, O.W., Hendricks, G.M. and Meyer, D.H. (1992)“Evidence that

Bismuth Salts Reduce Invasion of Epithelial Cells by Enteroinvasive Bacteria, Med. Microbiol. Immunol. 181:131-143.

4. Nadeau, O.W. and Carlson, G.M. (1994) Zero-length Conformation-dependent Cross-

linking of Phosphorylase Kinase Subunits by Transglutaminase, J. Biol. Chem. 269:29670-2967.

5. Nadeau, O.W., Falick, A. and Woodworth, R.C. (1996) Structural Evidence For An Anion-

Directing Track in the Hen Ovotransferrin N-Lobe: Implications for Transferrin Synergistic Anion-Binding, Biochemistry 35:14294-14303.

6. Nadeau, O.W., Sacks, D.B. and Carlson, G.M. (1997)“Differential Affinity Cross-Linking of

Phosphorylase Kinase

Conformers by the Geometric Isomers of Phenylenedimaleimide, J. Biol. Chem. 272:26196-26201.

7. Domanski, P., Fish, E., Nadeau, O.W., Witte, M., Platanias, L.C., Yan, H., Krolewski, J.,

Pitha, P. and Colamonici,

O.R. (1997) A Region of the β Subunit of the Interferon a Receptor Different from Box 1 Interacts With Jak1 and Is Sufficient To Activate the Jak-Stat Pathway and Induce an Antiviral State, J. Biol. Chem. 272:26388-26393.

8. Nadeau, O.W., Sacks, D.B. and Carlson, G.M. (1997) The Structural Effects of

Endogenous and Exogenous

Ca2+/Calmodulin on Phosphorylase Kinase, J. Biol. Chem. 272:26202-26209.

9. Domanski, P., Nadeau, O.W., Platanias, L.C., Fish, E., Kellum, M., Pitha, P. and

Colamonici, O.R. (1998) Differential Use of the βL Subunit of the Type I Interferon Receptor Determines Signaling Specificity for IFNα2 and IFNβ, J. Biol. Chem. 273:3144-3147.

10. Platanias, L.C., Domanski, P., Nadeau, O.W., Yi, T., Uddin, S., Fish, E., Neel, B.G. and

Colamonici, O.R. (1998)

Identification of a Domain in the β Subunit of the Type I Interferon (IFN) Receptor that Exhibits a Negative

Regulatory Effect in the Growth Inhibitory Action of Type I IFNs, J. Biol. Chem. 273:5577-5581.

11. Ayers, N.A., Nadeau, O.W., Read, M.W., Ray, P. and Carlson, G.M. (1998)“Effector-

sensitive Cross-linking of

Phosphorylase-b Kinase by the Novel Cross-linker 4-phenyl-1,2,4-triazoline-3,5-dione, Biochem. J. 331:137-141.

12. Nadeau, O.W., Traxler, K.W. and Carlson, G.M. (1998) Zero-length Cross-linking of the β

Subunit of Phosphorylase Kinase to the N-terminal Half of its Regulatory α Subunit, Biochem. Biophys. Res. Commun. 251:637-641.

223

13. Nadeau, O.W., Traxler, K.W., Fee, L.R., Baldwin, B.B. and Carlson, G.M. (1999)

Activators of Phosphorylase Kinase Alter the Cross-linking of its Catalytic Subunit to the C-terminal 1/6th of its Regulatory α Subunit, Biochemistry 38:2551-2559.

14. Carlson, G.M. and Nadeau, O.W.(1999) Bifunctional Crosslinking Reagents, in The

Encyclopedia of Molecular

Biology, Ed. T.E. Creighton, Wiley, New York, pp 279-28.

15. Carlson, G.M. and Nadeau, O.W.(1999) Crosslinking, in The Encyclopedia of Molecular

Biology, Ed. T.E. Creighton, Wiley, New York, pp 582-584.

16. Carlson, G.M. and Nadeau, O.W. (1999) Enzyme Immobilization and Conjugation, in The

Encyclopedia of Molecular Biology, Ed. T.E. Creighton, Wiley, New York, pp 829.

17. Carlson, G.M. and Nadeau, O.W.(1999) “Gluteraldehyde,” in The Encyclopedia of

Molecular Biology, Ed. T.E.

Creighton, Wiley, New York, pp 1019-1020.

18. Nadeau, O.W., Domanski, P., Usacheva, A., Uddin, S., Platanias, L.C., Pitha, P., Raz, R., Levy, D., Majchrzak,

B., Fish, E. and Colamonici, O.R. (1999) The Proximal Tyrosines of the Cytoplasmic Domain of the β Chain of the Type I Interferon Receptor are Essential for Signal Transducer and Activator of Transcription (Stat) 2 Activation, J. Biol. Chem. 274:4045-4052.

19. Nadeau O. W., Carlson, G.M. and Gogol, E.P. (2002) A Ca2+-Dependent Global

Conformational Change in the

3D Structure of Phosphorylase Kinase Obtained from Electron Microscopy, Structure 10:23-32.

20. Rice, N.A., Nadeau, O.W., Yang, Q. and Carlson, G. M. (2002) The Calmodulin-binding

Domain of the Catalytic

γ Subunit of Phosphorylase Kinase Interacts with Its Inhibitory α Subunit, J. Biol. Chem. 277:14681-14687.

21. Nadeau, O.W. and Carlson, G.M. (2002) Chemical Crosslinking in Studying Protein-

protein Interactions, in

Protein-Protein Interactions, Chap. 6, Ed. Erica Golemis, Cold Spring Harbor Laboratory Press, New York, pp 75-91 .

22. Marshal, S, Nadeau, O.W. and Yamasaki, K. (2004) Dynamic Actions of Glucose and Glucosamine on ,

Hexosamine Biosynthesis in Isolated Adipocytes. J. Biol. Chem. 279: 35313-35319.

23. Priddy, T.S., MacDonald. B.A., Heller, W.T., Nadeau, O.W., Trewhella, J., and Gerald M. Carlson, (2005) Ca2+-

224

induced Structural Changes in Phosphorylase Kinase Detected by Small-angle X-ray

Scattering. Protein Sci 14,

1039-1048.

24. Nadeau, O. W., Gogol E. and Carlson, G. M. (2005) Cryoelectron Microscopy Reveals New Features in the

Three-dimensional Structure of Phosphorylase Kinase. Protein Sci 14, 914-920.

25. Marshall, S, Nadeau, O and Yamasaki, K, (2005) Glucosamine-induced Activation of Glycogen Biosynthesis in

Isolated Adipocytes: Evidence for a Rapid Allosteric Control Mechanism within the Hexoseamine Biosynthesis

Pathway, J. Biol. Chem. 280, 11018-11024.

26. Nadeau, O. W. and Carlson, G. M. (2005) Protein Interactions Captured by Chemical Cross-linking, in Protein-

Protein Interactions, 2nd ed, Chapter 7, Eds. Erica Golemis and Peter Adams, Cold Spring Harbor Laboratory

Press, New York pp 105-127.

27. Nadeau, O. W. (2006) Protein-protein interaction analysis: chemical cross-linking, in Encyclopedic Reference

of Genomics and Proteomics in Molecular Medicine, Eds. K. Ruckpaul and D. Ganten, Springer-Verlag,

Heidelberg, online version at http://www.springerlink.com/content/n4425664628508x7/fulltext.html.

28. Nadeau, O.W., Anderson, D.W., Yang, Q., Artigues, A., Paschall, J.E., Wyckoff, G.J., McClintock J.L., and

Carlson, G. M. (2007) Evidence for the Location of the Allosteric Activation Switch in the Multisubunit

Phosphorylase Kinase Complex from Mass Spectrometric Identification of Chemically Cross-linked Peptides.

J. Mol. Biol. 365, 1429-1445.

29. Owen W. Nadeau, Gerald J. Wyckoff, Justin E. Paschall, Antonio Artigues, Jessica Sage, Maria T. Villar and

Gerald M. Carlson, (2008) Cross Search, a User-friendly Search Engine for Detecting Chemically Cross-linked

Peptides in Conjugated Proteins, Mol. Cell. Proteomics, 7, 739-749.

30. Igor G. Boulatnikov, Owen W. Nadeau, Patrick J. Daniels, Jessica M. Sage, Marina D. Jeyasingham, Maria T.

225

Villar, Antonio Artigues, and Gerald M. Carlson, (2008) The Regulatory β

Subunit of Phosphorylase Kinase

Interacts with Glyceraldehyde-3-phosphate Dehydrogenase, Biochemistry 47, 7228 – 7236

31. Marina D. Jeyasingham, Antonio Artigues, Owen W. Nadeau and Gerald M. Carlson, (2008) Structural Evidence

for Co-Evolution of the Regulation of Contraction and Energy Production in Skeletal Muscle, J. Mol. Biol. 377,

623-629.

32. Tran, Q-K., Leonard, J., Black, D.J., Nadeau, O.W., Boulatnikov, I.G., and Persechini, A., (2009) Effects of

combined phosphorylation at Ser-617 and Ser-1179 in endothelial nitric-oxide synthase on EC50(Ca2+) values for

calmodulin binding and enzyme activation. J. Biol. Chem. 284, 11982-11899. PMID: 19251696

33. Boulatnikov, I.G., Peters, J.L., Nadeau, O.W., Sage, J.M., Daniels, P.J., Kumar, P., Walsh, D.A. and Carlson,

G.M. (2009) Expressed phosphorylase b kinase and its αγδ subcomplex as regulatory models for the rabbit

skeletal muscle holoenzyme. Biochemistry 48, 10183-10191. PMID: 19764815.

34. Nadeau, O.W., Liu, W., Boulatnikov, I.G., Sage, J.M., Peters, J.L. and Carlson, G.M. (2010) The glucoamylase

inhibitor acarbose is a direct activator of phosphorylase kinase. Biochemistry 49, 6505-6507.

Manuscripts in press: (Provide names of all authors, title, journal, and PDF of manuscript plus evidence of acceptance by journal) Manuscripts submitted but not yet accepted: (Provide names of all authors, title, journal, and PDF of manuscript plus evidence of receipt of manuscript by journal.)

Invited or non-peer-reviewed articles or reviews: (Provide names of all authors, year, title, journal, volume, and pages. If in press, provide documentation and PDF or article/review if published within the last five years.)

226

Books and book chapters: (Provide names of all authors, year, book title, chapter title, edition, publisher, and pages. If in press, provide documentation and PDF or book/book chapter if published the last five years.)

Nadeau, O.W. and Carlson, G.M. (2012) Methods for Detecting Structural Changes in Large Protein Complexes.

Methods in Molecular Biology, Vol. 796 (Allostery: Methods and Protocols; A.W. Fenton, Ed.), pp. 117-132, Humana Press, Totowa, NJ.

Published abstracts: (Provide names of all authors (applicant’s name bolded), year, title, where published, volume, and pages.) If the same work is reflected in a published abstract and a presentation or poster, the work must only be listed only once.

1. Nadeau, O.W. and Woodworth, R.C. (1990) A Bromopyruvate Affinity Labeled Peptide From The N-Terminal

Half-Molecule of Ovotransferrin, Poster session and program abstracts, Fourth Symposium of the Protein

Society.

2. Nadeau, O.W., Meyer, D.H. and Gump, D.W. (1990) Invasion of Epithelial Cells by

Enteroinvasive Escherichia

coli is Inhibited by Bismuth Subsalycilate, Poster session, American Society of Microbiology, Abstracts of annual

meeting.

3. Gump, D.W., Nadeau, O.W. and Meyer, D.H. (1990) Invasion of Epithelial Cells by

Yersinia enterocolitica is

Inhibited by Bismuth Subsalycilate, Program abstract of the 30th Interscience Conference Antimicrobial Agents &

Chemotherapy, Abstract 269.

4. Nadeau, O.W. and Carlson, G.M. (1993) Intramolecular Cross-linking of Phosphorylase

Kinase with

Transglutaminase, FASEB Journal, 7:A638.

5. Nadeau, O.W. , Sacks, D.B. and Carlson, G.M. (1995) Differential Affinity Cross-linking of

Phosphorylase Kinase

by Phenylenedimaleimide Isomers, Protein Science 4:147.

6. Nadeau, O.W., Sacks, D.B. and Carlson, G.M. (1995) Exogenous Calmodulin Promotes

Changes in the

Quaternary Structure of Phosphorylase Kinase, FASEB Journal 9:A1304

7. Nadeau, O.W. and Carlson, G.M. (1996) Ca2+ and Calmodulin Alter Interactions Between

the Catalytic Subunit of

Phosphorylase Kinase and the C-terminal Domain of its Regulatory α-subunit, FASEB Journal 10:A1268.

227

8. Ayers, N.A., Nadeau, O.W., Read, M.W., Ray, P. and Carlson, G.M. (1997) Chemical Cross-linking of

Phosphorylase-b Kinase by the Novel Cross-linker 4-phenyl-1,2,4-triazoline-3,5-dione, FASEB Journal 11:A1180.

9. Nadeau, O.W., Traxler, K.W. and Carlson, G.M. (1997) The β Subunit of Phosphorylase

Kinase Interacts with the

N-terminal Domain of its Regulatory α Subunit, Protein Science 6:146.

10. Nadeau, O.W., Carlson, G.M. and Gogol, E.P. (2001) Structural Evidence for a Global

Ca2+-dependent

Conformational Change in Phosphorylase Kinase, Protein Science 10:449.

11. Jeyasingham, M., Nadeau, O.W. and Carlson, G. M. (2002) Zero-length cross-linking of

the intrinsic catalytic

gamma subunit of phosphorylase kinase with either of its regulatory α or δ subunits is oppositely affected by Ca2+,

Protein Science 11:268.

12. Nadeau, O.W., Gogol, E.P. and Carlson, G. M. (2003) Cryo-electron Microscopy Reveals

New Features in the

3D-Structure of Phosphorylase kinase, Protein Science 12:156.

13. Nadeau, O.W., Anderson, D. B., Yang, Q., Artigues, A. and Carlson, G.M. (2004) The N-

terminus of the

regulatory β subunit of phosphorylase kinase mediates self-association of this subunit in the activated

holoenzyme complex, Protein Science 13, Abs. # 83, 77.

14. Nadeau, O.W., Paschall, J.E., Wyckoff, G.J., Artigues, A. and Carlson, G.M. (2005) A

data-based approach for

detecting protein-protein interactions by chemical cross-linking and mass spectrometry, Protein Science 14,

Abs. # 448, 215.

15. Nadeau, O.W., Paschall, J.E., Wyckoff, G.J., Artigues, A. and Carlson, G. (2006)

Mapping a Phosphorylation-

dependent Subunit Communication Network in the Hexadecameric Phosphorylase kinase Complex Using a

Data-based Search engine to Detect Chemically Cross-linked Peptides by Mass Spectrometry. Protein Science

15, Abs. # 457, 224.

16. Boulatnikov, I.G., Daniels, P.J., Nadeau, O.W., Kumar, P., Walsh, D.A., and Carlson,

G.M. (2006) Éxpressed αγδ

228

Subcomplex of the Phosphorylase b Kinase (PhK) Complex as a Model for Characterizing the PhK (αβγδ)4

Holoenzyme from Rabbit Skeletal Muscle. Protein Science 15, Abs. # 576, 268.

17. Owen W. Nadeau, Gerald J. Wyckoff, Justin E. Paschall, Antonio Artigues, Jessica

Sage, Maria T. Villar and

Gerald M. Carlson, (2007) Detection of Intrasubunit Interactions in the Regulatory β Subunit in the

Hexadecameric Phosphorylase Kinase Complex Using a Data-based Search Engine to Detect Chemically Cross-

linked Peptides by Mass Spectrometry, Protein Sci. 16 (Suppl. 1), 249

18. Owen W. Nadeau, Gerald J. Wyckoff, Justin E. Paschall, Antonio Artigues, Jessica

Sage, Maria T. Villar and

Gerald M. Carlson, (2007) Detection of Intrasubunit Cross-linking Interactions in the Regulatory Beta Subunit of

Phosphorylase Kinase Suggest a Possible Flip-flop Mechanism of Activation by Phosphorylation, Eighth

International Symposium on Mass Spectrometry in the Life Sciences, C.16

19. Owen W. Nadeau, Laura Lane, Carol V. Robinson and Gerald M. Carlson, (2010) Mass

spectrometry reveals a

subunit interaction map of the phosphorylase kinase complex, Protein Sci. 19 (Suppl. 1), 250.

20. Owen W. Nadeau, Weiya Liu, Dong Xu, Todd Funke, Jessica Sage, Yang Zhang and

Gerald M. Carlson, (2011)

The glucoamylase activity of the protein kinase phosphorylase kinase, Protein Sci. 20 (Suppl. 1), 180. Other scholarly publications

21. Presentations and posters in chronological order (oldest first, most recent last)

Oral paper presentations: (Provide names of all authors (applicant’s name bolded), title, sponsoring organization, extent of peer-review, and location and date of presentation.)

Scientific papers presented at national and international meetings: Scientific papers presented at local and regional meetings:

Poster presentations in chronological order (oldest first, most recent last): (Provide names of all authors (applicant’s name bolded), title, sponsoring organization, extent of peer-review and location and date of presentation.)

229

CURRICULUM VITAE

Kenneth Richard Peterson PERSONAL DATA Position: Professor Birthday: 5/15/56 Citizenship: United States Married, two children ADDRESS Department of Biochemistry and Molecular Biology MSN 3030 School of Medicine University of Kansas Medical Center 3901 Rainbow Boulevard Kansas City, Kansas 66160-7421 Tel.: (913) 588-6907 (Office) 588-5447 (Lab) 588-9896 (FAX) E-mail: kpeterson@kumc.edu EDUCATION Ph.D. University of Arizona, 1987 Dissertation advisor: Dr. David W. Mount

230

Dissertation topic: Characterization of mutations in the lexA gene of Escherichia coli K-12. M.S. Idaho State University, 1981 Thesis advisor: Dr. Larry D. Farrell Thesis topic: Attempted indirect induction of prophage lambda in E. coli

by nicked or gapped non-operator DNA. B.S. Northern Arizona University, 1979 Majors: Microbiology, Chemistry PROFESSIONAL APPOINTMENTS 2010 Director, Center for Epigenetics and Stem Cell Biology, Institute for

Reproductive Health and Regenerative Medicine, University of Kansas Medical Center, Kansas City, Kansas

2003- Vice-Chairman, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas

2003- Professor, Department of Biochemistry and Molecular Biology and Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas

1998-2003 Associate Professor, Department of Biochemistry and Molecular Biology and Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas

1996-1998 Research Associate Professor of Medicine, Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, Washington.

1996-1998 Adjunct Research Associate Professor of Genetics, Department of Genetics, University of Washington, Seattle, Washington.

1996 Adjunct Research Assistant Professor of Genetics, Department of Genetics, University of Washington, Seattle, Washington.

1992-1996 Research Assistant Professor of Medicine, Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, Washington.

1991-1997 Director, Phosphorimager Analysis Facility, Markey Molecular Medicine Center, University of Washington, Seattle, Washington.

1990-1992 Senior Fellow, Dr. George Stamatoyannopoulos, Division of Medical Genetics, University of Washington, Seattle, Washington.

1987-1990 Postdoctoral Research Associate, Dr. Margaret G. Kidwell, Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona.

1981-1986 Graduate Research Assistant, Dr. David W. Mount, Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona.

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1979-1981 Graduate Teaching Assistant, Department of Microbiology and

Biochemistry, Idaho State University, Pocatello, Idaho. MEMBERSHIP IN PROFESSIONAL SOCIETIES American Society of Hematology, 2001- American Association for Advancement of Science, 1993- American Society for Microbiology, 1980- American Society of Nephrology, 2004-2007 Genetics Society of America, 1988- International Society for Stem Cell Research, 2004- Sigma Xi, 1981- HONORS AND AWARDS Self Faculty Scholar award for research and mentoring, University of Kansas,

2001. Investigator Research Award, University of Kansas Medical Center, 2003. Fulbright Scholar grantee to Brazil, 2009-2010. SERVICE ACTIVITIES Departmental

Member, Graduate Committee, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 1998-present.

Member, Search Committee for Fluorescence Biochemist, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2003.

Member, Search Committee for Regulatory Biochemist , Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2003.

Chairman, Search Committees for Nucleic Acids Biochemist, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2003-2006.

232

Chairman, Graduate Committee, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2003-2004.

Member, Biochemistry and Molecular Biology Study Section, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2005-present.

Chairman, Appointments, Promotions and Tenure Committee, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2005-present.

Member, Search Committee for Senior Molecular Biologist, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2006-2007.

Member, BMB KLSIC Space Advisory Committee, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2007-present.

Participant, BMB Heartland Undergraduate Biochemistry forum, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2011-2012.

Institutional Member, Medical Thesis Committee, University of Washington, 1994-1998.

Faculty participant, Genetic Approaches to Aging Training Grant administered by Peter Rabinovitch and George M. Martin, Department of Pathology, University of Washington, 1996-1998.

Member, Committees to develop Program in Molecular Genetics and Therapeutics and Program in Developmental Biology, Task Force for Research, University of Kansas Medical Center, 1998, 2000.

Member, Graduate Faculty, University of Kansas Medical Center, 1998-present.

Member, Smith Mental Retardation and Developmental Disabilities Research Center (MRDDRC), University of Kansas Medical Center, 1998-2007.

Member, Advisory Board for the Transgenic and Genetic Technologies Support Facility, University of Kansas Medical Center, 1998-2004, 2005-present.

Co-Chair, 2009.

Chair, 2009-present.

Participant, Annual IGPBS Recruitment Weekend, University of Kansas Medical Center, 1999-2006.

Member, Harry R. Wahl Academic Society, University of Kansas Medical Center, 1999-2005.

233

Judge, Student Research Forum, University of Kansas Medical Center, 1999, 2002-2004, 2006-2010.

Member, Subcommittee to examine microarray technology at KUMC, 2000.

Member, Graduate Travel Committee, University of Kansas Medical Center, 2000-2005.

Member, Laboratory Animal Resources Review Committee, University of Kansas Medical Center, 2000.

Member, Laboratory Animal Resources Director Search Committee, University of Kansas Medical Center, 2000-2001.

Elected member, School of Medicine Research Committee, University of Kansas Medical Center, 2000-2003.

Vice-Chairman, 2001-2002.

Chairman, 2002-2003.

Member, Faculty Council, University of Kansas Medical Center, 2000-2003, 2004-2010.

Vice Chair-elect, 2008-2009.

Vice Chair, 2009-2010.

Member, Microarray Core Advisory Committee, University of Kansas Medical Center, 2001.

Member, Molecular Virologist Faculty Search Committee, Dept. Microbiology, Molec. Genetics & Immunology, University of Kansas Medical Center, 2002.

Member, Internal Advisory/Planning Committee, KUMC Center for Molecular Informatics, University of Kansas Medical Center, 2002.

Member, Medical Genetics Subcommittee of the Education Council, University of Kansas Medical Center, 2002-2004.

Member, Faculty Assembly Research Committee, University of Kansas Medical Center, 2002-2003.

Member, Executive Committee of the Medical Faculty, University of Kansas Medical Center, 2002-2003, 2004-2005, 2008-2010.

Member, Internal Advisory Committee, Institute of Maternal Fetal Biology, University of Kansas Medical Center, 2002-2010.

Member, Self Graduate Fellowship Selection Committee, University of Kansas, 2002-2004.

234

Member, Research Advisory Group, University of Kansas Medical Center, 2003.

Member, Admissions/Prematriculation Working Group, Medical Education Review, University of Kansas Medical Center, 2003.

Chairman, W. S. Sutton and Reathea Mae Resco Scholarships Selection Committee, University of Kansas Medical Center, 2003.

Member, Interdepartmental Graduate Program in Biological Sciences Advisory Board, University of Kansas Medical Center, 2003-2004.

Member, Medical Center Hearings Committee, University of Kansas Medical Center, 2004.

Member, Advisory Committee for the Molecular Resource Facility, University of Kansas Medical Center, 2004.

Member, School of Medicine Space Committee, University of Kansas Medical Center, 2004.

Member, Viral Pathogenesis Faculty Search Committee, Dept. Microbiology, Molec. Genetics & Immunology, University of Kansas Medical Center, 2005-2006.

Full member, Kansas Masonic Cancer Research Institute (KMCRI), Basic Science Research Program/Risk Factors for Carcinogenesis, University of Kansas Medical Center, 2005-2009.

Associate member, University of Kansas Cancer Center (KUCC), Risk Factors for Carcinogenesis Research Program, University of Kansas Medical Center, 2009-present.

Member, Facility Director Search Committee, Transgenic and Gene Targeting Facility, University of Kansas Medical Center, 2005.

Member, Cancer/Developmental Biology Search Committee, Dept. Molecular & Integrative Physiology, University of Kansas Medical Center, 2005-2006.

Member, Peter T. Bohan Lecture Selection Committee, University of Kansas Medical Center, 2005.

Member, Cancer and Developmental Biology Seminar Series Committee, Institute of Maternal-Fetal Biology, University of Kansas Medical Center, 2005.

Chair, Institutional Human Stem Cell Research Oversight Committee, University of Kansas Medical Center, 2006-present.

Reviewer, Biomedical Research Training Program, University of Kansas Medical Center, 2007.

Primary Mentor, Department of Pediatrics Faculty Mentoring Program, University of Kansas Medical Center, 2007.

235

Design Director, siRNA Library Core, University of Kansas Cancer Center (KUCC), University of Kansas Medical Center, 2007-2009.

Member, Bioinformatics Search Committee, University of Kansas Medical Center, 2008.

Co-Director, IGPS Module 3: Molecular Biology course, University of Kansas Medical Center, 2008-2009.

Member, KUMC Budget Advisory Committee, University of Kansas Medical Center, 2009.

Member, Furlough Plan Development Committee, University of Kansas Medical Center, 2009-2010.

Member, Executive Research Board, Institute for Reproductive Health and Regenerative Medicine, University of Kansas Medical Center, 2010-present.

Member, High Throughput Genomics Facility Advisory Committee, University of Kansas Medical Center, 2011-present.

Member, Kidney Institute Internal Executive Advisory Committee, University of Kansas Medical Center, 2012-present.

Member, LCME Educational Resources Self-Study Committee, University of Kansas Medical Center, 2012.

Professional

Extramural scientific reviews:

Grants:

Ad hoc reviewer, Wellcome Trust grants.

Ad hoc reviewer, National Science Foundation grants.

State of Louisiana Board of Regents Support Fund Research and Development Program, 2000.

External Advisory Committee, University of South Alabama Comprehensive Sickle Cell Center, 2001.

Member, Hall Grant Annual Progress Report Review Committee, Kansas City metropolitan area, 2002.

Ad hoc reviewer, NIH MBRS SCORE Program grant proposal, 2002-2003.

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Ad hoc reviewer, Muscular Dystrophy Campaign grant proposal, 2003.

Ad hoc member, NIH Hematopoiesis (HP) Study Section, 2003.

Ad hoc member, NIH Erythrocyte and Leukocyte Biology (ELB) Study Section, 2004.

Ad hoc member, NIDDK Grant Review Board ZDK1 GRB-B (M1) and ZDK1 GRB-B (M2), 2004.

Reviewer, UK Biotechnology and Biological Sciences Research Council grant proposal, 2004.

Ad hoc reviewer, Special Emphasis Panel ZRG1 HEME-B 02 M, Globin Gene Transcription, Hemostasis and Thrombosis (HT) Study Section, 2005.

Ad hoc reviewer, Special Emphasis Panel, ZRG1 HEME-B 02 M, Hemoglobin Stabilizing Protein, Hemostasis and Thrombosis (HT) Study Section, 2005.

Reviewer, Special Emphasis Panel, Assay Development for High Throughput Molecular Screening RFA, NINDS, 2006.

Ad hoc reviewer, Special Emphasis Panel, ZDK1 GRB-9 (J1), Hematopoietic Stem Cells, NIDDK, 2006.

Ad hoc reviewer, Special Emphasis Panel, ZDK1 GRB-6 (M3) 1, A Search for Genes that Regulate Stem Cells, NIDDK, 2008.

Reviewer, Netherlands Genomics Initiative Horizon Program grant proposal, 2008.

Ad hoc reviewer, PPG Special Emphasis Panel, HLBP 1 Workgroup 014, Cryopreserved Erythroblast Products for Human Transfusion, NHLBI, 2009.

Ad hoc reviewer, Special Emphasis Panel, ZHL1 CSR-R (O5), Grand Opportunity"(GO): Testing of Mechanistic Hypotheses Generated by Findings from Genetic and Genomic Studies of Heart, Vascular, Lung, and Blood Disorders, NHLBI, 2009.

Ad hoc reviewer, Special Emphasis Panel, ZHL1 CSR-R (O4), Grand Opportunity"(GO): Next Steps in Gene Discovery: Building upon GWAS, NHLBI, 2009.

Ad hoc reviewer, Special Emphasis Panel, ZHL CSR-R (F1), Patient Oriented Research Career Enhancement Awards, NHLBI, 2009.

Ad hoc reviewer, Special Emphasis Panel, ZRG1 VH-F (03) M, Erythrocyte Biology Teleconference, NIH CSR, 2010.

Ad hoc reviewer, Special Emphasis Panel, ZDK1 GRB-9 (M1), Hematology Program Projects Teleconference, NIDDK, 2010.

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Chair/Ad hoc reviewer, Special Emphasis Panel/Scientific Review Group 2011/01

ZRG1 CB-C (40) P, NIGMS P01 Internet Assisted Review, NIH CSR, 2010.

Ad hoc reviewer, Special Emphasis Panel/Scientific Review Group 2011/05 HLBP Workgroup 014, NHLBI P01 Review, NHLBI, 2011

Ad hoc reviewer, Special Emphasis Panel/Scientific Review Group 2011/05 ZDK1 GRB-6 (M3) 1, Hemoglobinopathies Program Projects Teleconference, NIDDK, 2011. Ad hoc member, NIH Molecular Genetics B (MGB) Study Section, 2012.

International Reviewer, Portuguese Foundation for Science and Technology, 2012.

Scientific Meetings:

Abstract reviewer, Thalassemia and Globin Gene Regulation Session, American Society of Hematology 50th Anniversary Annual Meeting, 2008

Journals:

Guest Editor, Anemia, Sickle Cell Disease: Genetics, Cellular and Molecular Mechanisms, and Therapies special issue, 2011-2012.

Ad hoc reviewer – American Journal of Hematology, Biochemical Genetics, Biochemical Journal, BioTechniques, Biology of the Neonate, Blood, Blood Cells, Molecules and Diseases, Cell, Cellular and Molecular Biology, Cellular and Molecular Biology Letters, British Journal of Haematology, Cellular and Molecular Biology, Developmental Dynamics, EMBO Journal, EMBO Reports, European Journal of Haematology, Experimental Hematology, Genesis, Gene Therapy, Genetics, Genomics, Haematologica, Human Molecular Genetics, Journal of Biological Chemistry, Journal of Cellular Biochemistry, Journal of Molecular Medicine, Molecular and Cellular Biology, Nature, Nature Protocols, Nucleic Acids Research, PLoS One, Proceedings of the National Academy of Sciences USA, Transgenic Research.

Outside

Lectures on Hematology and Microbiology, Assumption-St. Bridget School, Seattle, WA, 1992-1998.

Co-founder and co-chairman, Assumption-St. Bridget School Science Parent Club, 1997-1998. Organization received National Catholic Education

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Association annual national award for Parents in Partnership with the school, 1999-2000. Scientist mentor, Science Education Partnership, grant-funded teacher-scientist program of Fred Hutchinson Cancer Research Center, 1997, 1998.

Lectures on Molecular Biology and Genetics, Bentwood Elementary School, Overland Park, KS., 1999, 2000, California Trail Jr. High, Overland Park, KS., 2000.

Judge, Kansas Junior Academy of Science District 3 and Shawnee Mission School District Research and Development Forum, 1999, 2000, 2002-2004.

Judge, Shawnee Mission Research and Development Forum, 1999, 2000, 2002, 2003.

Judge, Science Pioneers Greater Kansas City Science & Engineering Fair, 2000-2004, 2006, 2009, 2012-2013.

Member, Proteomics Proposal Task Force, Kansas City Area Life Sciences Institute, 2001.

KUMC Representative, Kansas City Proteomics Consortium, Kansas City Area Life Sciences Institute, 2001-2002.

Lecture, Science Pioneers’ Saturday Science Seminar program, 2003.

Co-Chairman, Stem Cell Symposium, University of Kansas Medical Center and Stowers Institute for Medical Research, 2004.

Member, Program Committee, Stem Cells and Developmental Biology mini-symposium, Life Sciences Research Day, Kansas City Area Life Sciences Institute, 2004-2005.

Teacher and Mentor, NHLBI Summer Institute Program to Increase Diversity (SIPID) – Red Blood Cell Disorders, University of Texas at Dallas, 2007-2010.

Teacher and Mentor, PRIDE Summer Institute Programs to Increase Diversity among Individuals Engaged in Health-Related Research, Georgia Health Sciences University, 2011-2012.

Lecture on Gene Regulation, Center for Advanced Professional Studies (CAPS), Blue Valley School District, Overland Park, KS, 2012-2013.

RESEARCH SUPPORT Extramural: Current

239

PI: 1. 1 R01 DK081290-A1, 09/01/08 – 08/31/12 (No cost extension to 08/31/13),

$212,500 (Annual direct costs), $850,000 (Total direct costs), “Transactivation of Fetal Hemoglobin,” PI Kenneth Peterson.

2. 1 X01 MH100830-01, 12/01/12-11/30/13, No annual direct costs, “HTS for HbF Inducers in Human Beta-globin YAC Transgenic Mice Bone Marrow Cells,” PI Kenneth Peterson

Co-PI, Co-I, Consultant, or Mentor: 1. 2 R01 HL069234-06A2, 09/01/08 – 05/31/12 (No cost extension to

05/31/13), $285,250 (Annual direct costs), $75,000 (Subcontract to Dr. Peterson), “Gamma Globin Induction: Molecular and Cellular Based Strategies,” Co-investigator with Betty S. Pace, University of Texas at Dallas (5% effort).

2. 9 P20 GM104936-06, 09/01/12 – 06/30/17, $1,460,187 (Annual direct costs), $7,263,467 (Total direct costs), “Molecular Regulation of Cell Development and Differentiation,” Core C, Molecular Profiling Core Director for Dale R. Abrahamson(10% effort).

3. 8 P20 GM103549-06, 07/01/11 – 06/30/16, $1,499,831 (Annual direct costs), $7,499,155 (Total direct costs), “Nuclear Receptors in Liver Health and Disease,” Consultant to Hartmut Jaeschke, mentor to Luciano DiTacchio (4% effort).

Pending PI: 1. 1 R01 DK095448-01A1, 04/01/13 – 03/31/18, $250,000 (Annual direct

costs), $1,250,000 (Total direct costs), “Linkage Between Zinc Metabolism and Fetal Hemoglobin Synthesis,” PI Kenneth Peterson.

2. 1 R01 HL11126401-01A1, 07/01/13 – 06/30/18, $250,000 (Annual direct costs), $1,250,000 (Total direct costs), “Mechanisms of HbF Activation by Non-deletional HPFH,” PI Kenneth Peterson.

Co-PI, Co-I, Consultant, or Mentor: 1. 1 R01 HL117993-01, 04/01/13 – 03/31/18, $250,000 (Annual direct costs),

$1,250,000 (Total direct costs), “Transcriptional Regulation of Hematopoiesis by O-GlcNAcylation,” Multi-PI with Chad Slawson.

2. AHA 13GRNT16910016, 07/01/13 – 06/30/15, $71,500 (Annual direct costs), $143,000 (Total direct costs), “O-GlcNAcylation Regulates the Mi2/NuRD Gene Repressor Complex,” Multi-PI with Chad Slawson.

3. 1 R01 DK100595-01, 09/01/13 – 08/31/16, $200,000 (Annual direct costs), $600,000 (Total direct costs), “Regulation of Globin Gene Switching by O-GlcNAc Post-Translational Modification,” Multi-PI with Chad Slawson.

Past

240

PI: 1. P01 HL053750, 09/30/94 – 09/29/99, $84,179 (Annual direct costs),

$405,532 (Total direct costs), “Gene Therapy for Sickle Cell Disease,” Project 5, Production of a sickle cell mouse model, project PI Kenneth Peterson.

2. R01 DK053510, 02/1/98 – 01/31/02 (No cost extension to 01/31/03), $178,478 (Annual direct costs), $667,159 (Total direct costs), “Role of the LCR in Human β-globin Gene Regulation,” PI Kenneth Peterson.

3. P50 DK057301, 09/30/99 – 08/31/01 (No cost extension to 08/31/02), $50,000 (Annual direct costs), $100,000 (Total direct costs), “Kansas Interdisciplinary Center for PKD Research,” Project 6, Test of the two-hit hypothesis in a PKD mouse model, project PI Kenneth Peterson.

4. 3 R01 HL067336-03S1 supplement, 09/22/03 – 05/31/05, $50,000 (Annual direct costs), $100,000 (Total direct costs), “Regulation of Globin Gene Switching in Human ES Cells,” PI Kenneth Peterson.

5. 5 R01 HL067336, 06/15/01 – 05/31/05 (no-cost extension to 05/31/07), $225,000 (Annual direct costs), $900,000 (Total direct costs), “Locus-linked Regulatory Motifs of Globin Gene Switching,” PI Kenneth Peterson.

6. 5 R01 DK061804, 09/30/01 – 05/31/05 (no-cost extension to 05/31/07), $200,000 (Annual direct costs), $800,000 (Total direct costs), “Molecular Control of Fetal γ-globin Gene Expression,” PI Kenneth Peterson.

7. Self Faculty Scholar Award, 07/1/01 – 06/30/04 (no cost extension to 06/30/07) $50,000 (Annual direct costs), $150,000 (Total direct costs), "Studies of β-like Globin Gene Switching," PI Kenneth Peterson.

8. 1 R56 DK081290, 09/24/07 – 08/31/08, $75,000 (Annual direct costs), $75,000 (Total direct costs), “Transactivation of Fetal Hemoglobin,” PI Kenneth Peterson.

9. AHA 0850193Z, 01/01/08 – 12/31/09, $71,500 (Annual direct costs), $143,000 (Total direct costs), “TSPYL1: A Therapeutic Target for Treating SCD-related Stroke,” PI Kenneth Peterson.

10. 3 R01 DK081290-02S1, 01/18/10 – 12/31/10, $77,553 (Total direct costs), “Transactivation of Fetal Hemoglobin, ARRA Administrative Supplement” PI Kenneth Peterson.

11. 2 R56 HL067336-05A2, 07/15/08-06/30/11, $250,000 (Annual direct costs), $250,00 (Total direct costs), “Locus-linked Regulatory Motifs of Globin Gene Switching,” PI Kenneth Peterson.

12. 3 R01 DK081290-01A1S1, 07/01/09 – 10/31/11, $14,240 (Annual direct costs), $24,480 (Total direct costs), “Transactivation of Fetal Hemoglobin, ARRA Administrative Supplement for Students” PI Kenneth Peterson.

Co-PI, Co-I, Consultant, or Mentor: 1. R01 DK030852, 06/01/95 – 05/31/99, $328,700 (Annual direct costs),

$1,314,800 (Total direct costs), “Studies of Globin Gene Switching and Erythroid Differentiation,” Co-investigator with Thalia Papayannopoulou.

2. R01 HL046557, 12/01/95 – 11/30/99, $180,640 (Annual direct costs), $722,560 (Total direct costs), “Biological Properties and Regulation of Stem Cells,” Co-Investigator with Thalia Papayannopoulou.

241

3. R01 HL020899, 04/01/97 – 03/31/02, $281,508 (Annual direct costs),

$1,529,085 (Total direct costs), “Cellular Mechanisms of HbF Regulation,” Co-Investigator with George Stamatoyannopoulos and Qiliang Li.

4. R01 DK045365, 08/01/97 – 07/31/02, $293,430 (Annual direct costs), $1,589,309 (Total direct costs), “Molecular Control of Globin Gene Switching,” Co-Investigator with George Stamatoyannopoulos and Qiliang Li.

5. 2 U54 HD033994, 04/01/01 – 03/31/06, $114,398 (Annual direct costs), $607,355 (Total direct costs), "Center for Reproductive Sciences," Project 1, Regulation of SF1 in the Gonads, Co-investigator with Leslie L. Heckert (5% effort).

6. 3 P01 HD039878-03S1 supplement, 04/01/04 - 03/31/06, $75,000 (Annual direct costs), $150,000 (Total direct costs), “Biology at the Maternal-Fetal Interface,” Co-investigator with Michael J. Soares (5% effort).

7. FY2006-056 COBRE Center for Cancer Experimental Therapeutics Project Award, 12/01/05 – 04/30/06, $52,000 (Annual direct costs), “Phenotypic screens of the TGFβ tumor suppressor pathway in mouse ES cells,” Mentor to Jay L. Vivian.

8. 5 R01 AR047233, 08/01/00 – 07/31/05 (no-cost extension to 07/31/06), $188,000 (Annual direct costs), $940,000 (Total direct costs), "Hoxc13 and Hair Follicle Morphogenesis," Co-investigator with Alan R. Godwin (5% effort).

9. P50 DK057301, 09/30/99 – 08/31/05 (no-cost extension to 08/31/06), $144,567 (Annual direct costs), $675,356 (Total direct costs), “Kansas Interdisciplinary Center for PKD Research,” Project 3, Polycystin G-protein signal transduction, Co-investigator with James P, Calvet (5% effort).

10. 5 R01 DK063975, 03/31/02 – 01/31/08, $189,636 (Annual direct costs), $984,936 (Total direct costs), “A Mouse Model of Acrodermatitis Enteropathica,” Co-investigator with Glen K. Andrews (10% effort).

11. 5 P50 DK057301, 09/30/05 – 08/31/10, $137,500 (Annual direct costs), $750,000 (Total direct costs), “Kansas Interdisciplinary Center for PKD Research,” Project 2, Polycystin-1 mediated calcium and cAMP signaling, Consultant to James P. Calvet (5% effort).

12. 1 P20 RR021940, 06/01/06 – 04/30/11, $1,499,831 (Annual direct costs), $7,499,155 (Total direct costs), “Nuclear Receptors in Liver Health and Disease,” Consultant to Curtis D. Klaassen, mentor to Grace L. Guo and Partha Krishnamurthy (8% effort).

13. 1 P20 RR024214, 09/27/07 – 06/30/12, $1,500,000 (Annual direct costs), $7,500,000 (Total direct costs), “Molecular Regulation of Cell Development and Differentiation,” Core C, Molecular Biology Core Director for Dale R. Abrahamson and mentor to Patrick E. Fields (20% effort).

Internal: Current:

242

Past: 1. Shared Use Biomedical Research Equipment, 11/22/99, $48,660,

“Purchase of a Qualitative and Quantitative PCR LightCycler System,” University of Kansas Medical Center, Co-Applicant with Glen K. Andrews.

2. Shared Use Biomedical Research Equipment, 11/20/03, $2,532, “Purchase of Two Vertical Laminar Airflow Workstations in the Transgenic and Gene-targeting Institutional Facility,” University of Kansas Medical Center, Co-Applicant with Wenhao Xu and Alan R. Godwin.

3. P20 Proposal Development, 08/05/04, $18,750, “Midwest Regional Exploratory Center for Human Embryonic Stem Cell Research,” KUMC Research Institute, University of Kansas Medical Center.

4. Faculty Travel Award, 10/13/06, $779, 15th Conference on Hemoglobin Switching, KUMC Research Institute, University of Kansas Medical Center.

5. Bridging Grant Winter 2006 RFP, 04/01/06 – 03/31/07, $35,000, “Molecular Control of Fetal γ-globin Gene Expression,” KUMC Research Institute, University of Kansas Medical Center.

6. Bridging Grant RFP, 04/18/06 – 04/30/07, $35,000, “Molecular Control of Fetal γ-globin Gene Expression – Selection of Transactivators,” K-INBRE, University of Kansas Medical Center.

7. Faculty Travel Award, 09/24/07, $235, 49th American Society of Hematology Annual Meeting, University of Kansas Medical Center.

8. Faculty Travel Award, 10/10/08, $346, 50th American Society of Hematology Annual Meeting, University of Kansas Medical Center.

9. Bridging Grant Fall 2007 RFP, 01/01/08 – 12/31/08, $35,000, “Transactivation of Fetal Hemoglobin,” KUMC Research Institute, University of Kansas Medical Center.

10. Faculty Travel Award, 09/14/10,$700, 17th Hemoglobin Switching Conference, KUMC Research Institute, University of Kansas Medical Center.

11. Lied Endowed Basic Science Program, 02/01/10 – 01/31/11, $35,000, “Transcriptional Regulation of the LAMA5 Gene,” University of Kansas Medical Center, Co-investigator with Dale R. Abrahamson.

Pending: PUBLICATIONS (88 total) Papers in Peer Reviewed Journals

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1. Peterson, K. R., K. F. Wertman, D. W. Mount, and M. G. Marinus. 1985.

Viability of Escherichia coli DNA adenine methylase (dam) mutants requires increased expression of specific genes in the SOS regulon. Mol. Gen. Genet. 201:14-19.

2. Peterson, K. R. and D. W. Mount. 1986. Differential repression of SOS

genes by unstable LexA41 (Tsl-1) protein causes a "split-phenotype" in Escherichia coli K-12. J. Mol. Biol. 193:27-40.

3. Peterson, K. R., N. Ossanna, and D. W. Mount. 1988. The Escherichia

coli K-12 lexA2 gene encodes a hypo-cleavable repressor. J. Bacteriol. 170:1975-1977.

4. Ennis, D. G., K. R. Peterson, and D. W. Mount. 1988. Increased

expression of the Escherichia coli umuDC operon restores SOS mutagenesis in lexA41 cells. Mol. Gen. Genet. 213:541-544.

5. de Frutos, R., K. Kimura, and K. R. Peterson. 1989. In situ hybridization

of Drosophila polytene chromosomes with digoxigenin-dUTP labeled probes. Trends in Genetics 5:336.

6. Daniels, S. D., K. R. Peterson, L. D. Strausbaugh, M. G. Kidwell, and A.

Chovnick. 1990. Evidence for horizontal transmission of the P transposable element between Drosophila species. Genetics 124:339-355.

7. de Frutos, R., K. Kimura, and K. R. Peterson. 1990. In situ hybridization

of Drosophila polytene chromosomes with digoxigenin-dUTP labeled probes. Methods Mol. Cell. Biol. 2:32-36.

8. Houck, M., J. Clark, K. R. Peterson, and M. G. Kidwell. 1991. Possible

horizontal transfer of Drosophila genes by the mite Proctolaelaps regalis. Science 253:1125-1129.

9. Wojciechowski, M. F., K. R. Peterson, and P. E. Love. 1991. Regulation

of the SOS response in Bacillus subtilis: Evidence for a LexA repressor homolog. J. Bacteriol. 173:6489-6498.

10. de Frutos, R., K. R. Peterson, and M. G. Kidwell. 1991. Distribution of

Drosophila melanogaster transposable element sequences in species of the obscura group. Chromosoma 101:293-300.

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11. Gnirke, A., C. Huxley, K. Peterson, and M. V. Olson. 1993. Microinjection

of intact 200-500 kb fragments of YAC DNA into mammalian cells. Genomics 15:659-667.

12. Peterson, K. R., C. H. Clegg, C. Huxley, B. M. Josephson, H. S. Haugen,

T. Furukawa, and G. Stamatoyannopoulos. 1993. Transgenic mice containing a 248 kb human β locus yeast artificial chromosome display proper developmental control of human globin genes. Proc. Natl. Acad. Sci. USA 90:7593-7597.

13. Peterson, K. R. and G. Stamatoyannopoulos. 1993. Role of gene order in

the developmental control of human γ and β globin gene expression. Mol. Cell. Biol. 13:4836-4843.

14. Peterson, K. R. and D. W. Mount. 1993. Analysis of the genetic

requirements for viability of Escherichia coli K-12 DNA adenine methylase (dam) mutants. J. Bacteriol. 175:7505-7508.

15. Peterson, K. R., G. Zitnik, C. Huxley, C. H. Lowrey, A. Gnirke, K. A.

Leppig, T. Papayannopoulou, and G. Stamatoyannopoulos. 1993. Use of YACs for studying control of gene expression: Correct regulation of the genes of a human β-globin locus YAC following transfer to mouse erythroleukemia cell lines. Proc. Natl. Acad. Sci. USA 90:11207-11211.

16. Pace, B., Q. Li, K. Peterson, and G. Stamatoyannopoulos. 1994. α-amino butyric acid fails to reactivate the totally silenced γ gene of the β locus YAC transgenic mouse. Blood 84:4344-4353.

17. Zitnik, G., K. R. Peterson, G. Stamatoyannopoulos, and T.

Papayannopoulou. 1995. Effects of butyrate and glucocorticoids on γ to β globin gene switching in somatic cell hybrids. Mol. Cell. Biol. 15:790-795.

18. Peterson, K. R., Q. Li, C. H. Clegg, T. Furukawa, P. A. Navas, E. J. Norton,

T. G. Kimbrough, and G. Stamatoyannopoulos. 1995. Use of yeast artificial chromosomes (YACs) in studies of mammalian development: Production of β-globin locus mice carrying human globin developmental mutants. Proc. Natl. Acad. Sci. USA 92:5655-5659.

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19. Furukawa, T., P. A. Navas, B. M. Josephson, K. R. Peterson, T.

Papayannopoulou, and G. Stamatoyannopoulos. 1995. Coexpression of ε, Gγ and Aγ globin mRNA in embryonic red blood cells from a single copy β-YAC transgenic mouse. Blood Cells, Molecules, & Disease 21:168-178.

20. Loring, J. F., C. Paszty, A. Rose, T. K. McIntosh, H. Murai, J. E. S. Pierce,

S. R. Schramm, E. M. Rubin, V. M.-Y. Lee, J. Q. Trojanowski, and K. R. Peterson. 1996. Rational design of an animal model for Alzheimer’s disease: Introduction of multiple human genomic transgenes to reproduce AD pathology in a rodent. Neurobiol. Aging 17:173-182.

21. Yang, Y., K. R. Peterson, T. Papayannopoulou, and G.

Stamatoyannopoulos. 1996. Human CD34+ cell EST database: Single pass sequencing of 402 clones from a directional cDNA library. Exptl. Hematology 24:605-612.

22. Peterson, K. R., C. H. Clegg, P. A. Navas, E. J. Norton, T. G. Kimbrough,

and G. Stamatoyannopoulos. 1996. Effect of deletion of 5’HS3 or 5’HS2 of the human β-globin locus control region on the developmental regulation of globin gene expression in β-globin locus yeast artificial chromosome transgenic mice. Proc. Natl. Acad. Sci. USA 93:6605-6609.

23. Li, Q., C. Clegg, K. Peterson, N. Raich, and G. Stamatoyannopoulos.

1997. Regulation of the human β-globin locus by human GATA-1 as measured in a binary transgenic mouse model. Proc. Natl. Acad. Sci. USA 94:2444-2448.

24. Blau, C. A., K. R. Peterson, J. G. Drachman, and D. Spencer. 1997. A

proliferative switch for genetically modified cells. Proc. Natl. Acad. Sci. USA 94:3076-3081.

25. La Spada, A. R., K. R. Peterson, S. A. Meadows, M. E. McClain, G. Jeng,

R. S. Chmelar, H. A. Haugen, K. Chen, M. J. Singer, D. Moore, B. J. Trask, K. H. Fischbeck, C. H. Clegg, and G. S. McKnight. 1998. Androgen receptor YAC transgenic mice carrying CAG 45 alleles show trinucleotide repeat instability. Human Mol. Genet. 7:959-967.

26. Navas, P. A., K. R. Peterson, Q. Li, E. Skarpidi, A. Rohde, S. E. Shaw, C.

H. Clegg, H. Asano, and G. Stamatoyannopoulos. 1998. Developmental specificity of the interaction between the locus control region and embryonic or fetal globin genes in transgenic mice with an HS3 core deletion. Mol. Cell. Biol. 18:4188-4196.

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27. Peterson, K. R. 1998. Rapid screening of recombinant DNA clones using

a single colony micro-lysate/restriction enzyme analysis protocol. Biotechniques 25:26-28.

28. Peterson, K. R., P. A. Navas, Q. Li, and G. Stamatoyannopoulos. 1998.

LCR-dependent gene expression in β-globin YAC transgenics: Detailed structural studies validate functional analysis even in the presence of fragmented YACs. Human Mol. Genet. 7:2079-2088.

29. Vassilopoulos, G., P. A. Navas, E. Skarpidi, K. R. Peterson, C. H. Lowrey,

T. Papayannopoulou, and G. Stamatoyannopoulos. 1999. Correct function of the locus control region may require passage through a nonerythroid cellular environment. Blood 93:703-712.

30. Papayannopoulou, T., G. V. Priestly, A. Rohde, K. R. Peterson, and B.

Nakamoto. 2000. Hemopoietic lineage commitment decisions: In vivo evidence from a transgenic mouse model harboring µLCR-βpro-lacZ as a transgene. Blood 95:1274-1282.

31. Perkins, A. C., K. R. Peterson, G. Stamatoyannopoulos, H. E. Witkowska,

and S. H. Orkin. 2000. Fetal expression of a human Aγ globin transgene rescues globin chain imbalance but not hemolysis in EKLF null mouse embryos. Blood 95:1827-1833.

32. Poorkaj, P., K. R. Peterson, and G. D. Schellenberg. 2000. Single-step

conversion of P1 and P1 artificial chromosome clones into yeast artificial chromosomes. Genomics 68:106-110.

33. Harju, S. J. and K. R. Peterson. 2001. Sensitive ribonuclease protection

assay employing glycogen as a carrier and a single inactivation/precipitation step. BioTechniques 30:1198-1204.

34. Navas, P. A., K. R. Peterson, Q. Li, M. McArthur, and G.

Stamatoyannopoulos. 2001. The 5'HS4 core element of the human β-globin locus control region is required for high level β-globin gene expression in definitive erythroid cells. J. Mol. Biol. 312:17-26.

35. Navas, P. A., Q. Li, K. R. Peterson, R. A. Swank, A. Rohde, J. Roy, and G.

Stamatoyannopoulos. 2002. Activation of the β-like globin genes is dependent on the presence of the β-locus control region. Human Mol. Genet. 11:893-903.

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36. Rodova, M., M. R. Islam, K. R. Peterson, and J. P. Calvet. 2003.

Remarkable sequence conservation of a small intron in the PKD1 gene. Mol. Biol. Evol. 20:1669-1674.

37. Navas, P. A., R. Swank, M. Yu, K. R. Peterson, and G.

Stamatoyannopoulos. 2003. Mutation of a transcriptional motif of a distant regulatory element reduces the expression of embryonic and fetal globin genes. Human Mol. Genet. 12:2941-2948.

38. Peterson, K. R., H. Fedosyuk, L. Zelenchuk, B. Nakamoto, E. Yannaki, G.

Stamatoyannopoulos, S. Ciciotte, L. L. Peters, L. M. Scott, and T. Papayannopoulou. 2004. Transgenic Cre expression mice for generation of erythroid-specific gene alterations. Genesis 39:1-9.

39. Harju, S. J., H. Fedosyuk, and K. R. Peterson. 2004. Rapid isolation of

yeast genomic DNA: Bust n’ Grab. MCB Biotechnol. 4:8-13. 40. Fang, X., J. Sun, P. Xiang, M. Yu, P. A. Navas, K. R. Peterson, G.

Stamatoyannopoulos, and Q. Li. 2005. Synergistic and additive properties of the β-globin LCR revealed by 5'HS3 deletion mutations: Implication on LCR chromatin architecture. Mol. Cell. Biol. 25:7033-7041.

41. Karpova, T., J. Presley, R. R. Manimaran, S. P. Scherrer, L. Tajeda, K. R.

Peterson, and L. L. Heckert. 2005. A Ftz-F1-containing yeast artificial chromosome recapitulates expression of steroidogenic factor 1 in vivo. Molec. Endocrinol. 19:2549-2563.

42. Harju, S. J., P. A. Navas, G. Stamatoyannopoulos, and K. R. Peterson.

2005. Genome architecture of human β-globin locus affects developmental regulation of gene expression. Mol. Cell. Biol. 25:8765-8778.

43. Blau, C. A., C. F. Barbas, III, A. Bomhoff, R. Neades, J. Yan, P. A. Navas,

and K. R. Peterson. 2005. γ-globin gene expression in CID-dependent multi-potential cells established from β-YAC transgenic mice. J. Biol. Chem. 280:36642-36647.

44. Navas, P. A., Q. Li, K. R. Peterson, and G. Stamatoyannopoulos. 2006.

Investigations of a human embryonic globin gene silencing element using YAC transgenic mice. Exp. Biol. Med. 231:328-334.

45. de Andrade, T. G., K. R. Peterson, A. F. Cunha, L. S. Moreira, A. Fattori,

S. T. O. Saad, and F. F. Costa. 2006. Identification of novel candidate

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genes for globin regulation in erythroid cells containing large deletions of the human β-globin gene cluster. Blood Cells, Mol. & Dis. 37:82-90.

46. Fedosyuk, H. and K. R. Peterson. 2007. Deletion of the human β-globin

LCR 5’HS4 or 5’HS1 differentially affects β-like globin gene expression in β-YAC transgenic mice. Blood Cells, Mol. Dis.39:44-55.

47. Peterson, K. R. 2007. Preparation of YAC DNA for transgenesis of mice.

Nature Protocols 2:3009-3015. 48. Harju-Baker, S.*, F. C. Costa*, H. Fedosyuk, R. Neades, and K. R.

Peterson. 2008. Silencing of Aγ-globin gene expression during adult definitive erythropoiesis by GATA-1-FOG-1-Mi2 complex binding at the -566 GATA site. Mol. Cell. Biol. 28:3101-3113. PMCID: PMC2423176 *co-1st authors

49. Chin, J. Y., J. Y. Kuan, P. S. Lonkar, D. S. Krause, M. M. Seidman, K. R.

Peterson, P. E. Nielsen, R. Kole, and P. M. Glazer. 2008. Correction of a splice-site mutation in the β-globin gene stimulated by triplex-forming peptide nucleic acids. Proc. Natl. Acad. Sci. USA 105:13514-13519. PMCID: PMC2533221.

50. Desmarets, M., C. M. Cadwell, K. R. Peterson, R. Neades, and J. C.

Zimring. 2009. Minor histocompatibility antigens on transfused leukoreduced units of red blood cells induce bone marrow transplant rejection. Blood 114:2315-2322. PMCID: PMC2745850

51. Tschulena, U., K. R. Peterson, H. Fedosyuk, B. Gonzalez, and C. F.

Barbas III. 2009. Positive selection of DNA-protein interactions in mammalian cells through phenotypic coupling with retrovirus production. Nature Struct. Mol. Biol. 16:1195-1199. PMCID: PMC2880176

52. Heruth, D. P., T. Hawkins, M. I. Gibson, D. P. Logsdon, I. V. Sokolovsky, N.

N. Nsumu, S. L. Major, B. Fegley, G. M. Woods, K. B. Lewing, K. A. Neville, K. Cornetta, K. R. Peterson, and R. A. White. 2010. Mutation in erythroid specific transcription factor KLF1 causes hereditary spherocytosis in the Nan hemolytic anemia mouse model. Genomics 96:303-307.

53. Chen, J., K. R. Peterson, C. Iancu-Rubin, and J. J. Bieker. 2010. Design

of embedded chimeric peptide nucleic acids that efficiently enter and accurately target and reactivate gene expression in vivo. Proc. Natl. Acad. Sci. USA 107:16846-16851. PMCID: PMC2947906

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54. Banzon, V., V. Ibanez, K. Vaitkus, M.-A. Ruiz, K. Peterson, J. DeSimone,

and D. Lavelle. 2011. siDNMT1 increases γ-globin expression in chemical-inducer-of-dimerization (CID)-dependent mouse β-YAC bone marrow cells and in baboon erythroid progenitor cell cultures. Exptl. Hematol. 239:26-36.

55. Giardine, B., J. Borg, D. R. Higgs, K. R. Peterson, S. Philipsen, D. Maglott,

B. K. Singleton, D. J. Anstee, A. N. Basak, B. Clark, F. C. Costa, P. Faustino, H. Fedosyuk, A. E. Felice, A. Francina, M. V. E. Gallivan, M. Georgitsi, R. J. Gibbons, P. C. Giordano, C. L. Harteveld, J. D. Hoyer, P. Joly, E. Kanavakis, P. Kollia, S. Menzel, W. Miller, K. Moradkhani, J. Old, A. Papachatzopoulou, M. N. Papadakis, P. Papadopoulos, S. Pavlovic, M. Radmilovic, C. Riemer, I. Schrijver, M. Stojiljkovic, S. L. Thein, J. Traeger-Synodinos, R. Tully, T. Wada, J. Waye, C. Wiemann, B. Zukic, D. H. K. Chui, H. Wajcman, R. C. Hardison, and G. P. Patrinos. 2011. Systematic documentation of human genetic variation using the microattribution approach. Nature Genet. 43:295-301.

56. Steenhard, B. M., A. Zelenchuk, L. Stroganova, K. Isom, P. L. St. John, G.

K. Andrews, K. R. Peterson, and D. R. Abrahamson. 2011. Transgenic expression of human LAMA5 suppresses murine Lama5 mRNA and Laminin α5 protein deposition. PLoS ONE 6:e23926. PMCID: PMC3168496

57. Costa, F. C., H. Fedosyuk, R. Neades, J. Bravo de los Rios, C. F. Barbas

III, and K. R. Peterson. 2012. Induction of fetal hemoglobin in vivo mediated by a synthetic γ-globin zinc finger activator. Anemia 2012:e507894. doi:10.1155/2012/507894. PMCID: PMC3384929

58. Peterson, K. R., H. Fedosyuk, and S. Harju-Baker. 2012. LCR 5’

hypersensitive site specificity for globin gene activation within the active chromatin hub. Nucleic Acids Res. 40:11256-11269. doi:10.1093/nar/gks900. PMCID: PMC3526258

59. Costa, F. C., H. Fedosyuk, A. M. Chazelle, R. Y. Neades, and K. R.

Peterson. 2012. Mi2β is required for γ-globin gene silencing: Temporal assembly of a GATA-1-FOG-1-Mi2 repressor complex in β-YAC transgenic mice. PLoS Genet. 8:e1003155. doi:10.1371/journal.pgen.1003155. PMCID: PMC3527334

Reviews (Peer-reviewed) 1. Ossanna, N., K. R. Peterson, and D. W. Mount. 1986. Genetics of DNA

repair in Bacteria. Trends in Genetics 2:55-58.

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2. Ossanna, N., K. R. Peterson, and D. W. Mount. 1987. UV-inducible SOS

response in Escherichia coli. Photochem. and Photobiol. 45:905-908. 3. Peterson, K. R., N. Ossanna, A. T. Thliveris, D. G. Ennis, and D. W.

Mount. 1988. Derepression of specific SOS genes promotes DNA repair and mutagenesis in Escherichia coli. J. Bacteriol. 170:1-4.

4. McCormick, S. P. A., K. R. Peterson, R. E. Hammer, C. H. Clegg, and S.

G. Young. 1996. Generation of transgenic mice from yeast artificial chromosome DNA modified by gene targeting. Trends Cardiovasc. Med. 6:16-24.

5. Peterson, K. R., C. H. Clegg, Q. Li, and G. Stamatoyannopoulos. 1997.

Production of transgenic mice with yeast artificial chromosomes. Trends in Genetics 13:61-66.

6. Li, Q., S. Harju, and K. R. Peterson. 1999. Locus control regions: Coming

of age at a decade plus. Trends in Genetics 15:403-408. 7. Harju, S. J., K. J. McQueen, and K. R. Peterson. 2002. Chromatin

structure and control of β-like globin gene switching. Exptl. Biol. Med. 227:683-700.

8. Li, Q., K. R. Peterson, X. Fang, and G. Stamatoyannopoulos. 2002.

Locus control regions. Blood 100:3077-3086. 9. Peterson, K. R. 2003. Transgenic mice carrying yeast artificial

chromosomes. Expert Rev. Molec. Med. 5:1-25. 10. Peterson, K. R. 2003. Hemoglobin switching: looping along.

BloodMed.com December 24, 2003. Book Chapters and Invited Contributions 1. Mount, D. W., K. F. Wertman, K. R. Peterson, J. W. Little, B. E. Markham,

and J. E. Harper. 1983. Regulation of the SOS response of Escherichia coli by the lexA and recA genes. In: D. H. Hamer and M. J. Rosenberg (ed.), Gene Expression, Alan R. Liss, Inc., New York, N.Y., pp. 135-143.

2. Mount, D. W., K. F. Wertman, D. G. Ennis, K. R. Peterson, B. L. Fisher,

and G. Lyons. 1983. Genetic analysis of the SOS response of Escherichia

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coli. In: E. Friedberg and B. A. Bridges (ed.), Cellular Responses to DNA Damage, Alan R. Liss, Inc., New York, N.Y., pp. 343-352.

3. Peterson, K. R., A. K. Ganesan, and D. W. Mount. 1986. Differential

expression of SOS genes in an E. coli mutant producing unstable LexA protein enhances excision repair but inhibits mutagenesis. In: L. Grossman and A. C. Upton (ed.), Mechanisms of DNA Damage and Repair, Plenum Press, New York, N.Y., pp. 265-272.

4. Kidwell, M. G., K. R. Peterson, and S. B. Daniels. 1990. Evolution of P

elements in the genus Drosophila. In: M. T. Clegg and S. J. O'Brian (ed.), Molecular Evolution, Alan R. Liss, Inc., New York, N.Y., pp. 107-116.

5. Kidwell, M. G. and K. R. Peterson. 1991. Evolution of transposable

elements in Drosophila. In: L. Warren and H. Koprowski (ed.), New Perspectives on Evolution, Wiley-Liss, Inc., New York, N.Y., pp. 139-154.

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6. Peterson, K. R., C. H. Clegg, C. Huxley, B. M. Josephson, H. S. Haugen,

T. Furukawa, and G. Stamatoyannopoulos. 1993. Transgenic mice containing a 248-kilobase human β locus yeast artificial chromosome display proper developmental control of human globin genes. Trans. Assoc. Amer. Phys. 106:101-109.

7. Castro, O. L., L. S. Chicoye, J. Greenberg, J. Haynes, Jr., and K. R.

Peterson. (S. Hart, ed.) 1994. Brighter horizons for sickle cell disease. Patient Care 28:26-44.

8. Stamatoyannopoulos, G., Q. Li., C. Clegg, and K. R. Peterson. 1995.

Molecular control of globin gene switching. In: Y. Beuzard, B. Lubin, and J. Rosa (ed.), Sickle cell disease and thalassemias: New trends in therapy, John Libbey Eurotext, Montrouge, France, pp. 3-8.

9. Peterson, K. R., C. H. Clegg, Q. Li, P. A. Navas, E. J. Norton, K. A. Leppig,

and G. Stamatoyannopoulos. 1995. Analysis of hemoglobin switching in transgenic mice using β-globin locus yeast artificial chromosomes. In: G. Stamatoyannopoulos (ed.), Proceedings of the. Ninth Conference on Hemoglobin Switching, Intercept Ltd., U.K., pp. 45-58.

10. Papayannopoulou, Th., G. Zitnick, K. R. Peterson, and G.

Stamatoyannopoulos. 1995. Analysis of hemoglobin switching in somatic cell hybrids. In: G. Stamatoyannopoulos (ed.), Proceedings of the. Ninth Conference on Hemoglobin Switching, Intercept Ltd., U.K., pp. 291-298.

11. Peterson, K. R. 1997. Production and analysis of transgenic mice containing

yeast artificial chromosomes. In: J. K. Setlow (ed.), Genetic Engineering, Principles and Methods, Vol. 19, Plenum Press, N. Y., pp. 235-255.

12. Li, Q., K. R. Peterson, and G. Stamatoyannopoulos. 1998. Developmental control

of ε- and γ-globin genes. Ann. N. Y. Sci. 850:10-17. 13. Peterson, K. R., P. A. Navas, and G. Stamatoyannopoulos. 1998. β-YAC

transgenic mice for studying LCR function. Ann. N. Y. Sci. 850:28-37. 14. Peterson, K. R. 1999. Use of YACs to express genes in transgenic mice.

Methods Enzymol. 306:186-203. 15. Jakobovits, A., B. T. Lamb, and K. R. Peterson. 1999. Production of transgenic

mice with yeast artificial chromosomes. In: R. S. Tuan and C. W. Lo (ed.), Methods in Molecular Biology, Vol. 136: Developmental Biology Protocols, Vol. II, Humana Press, Totowa, N. J., pp. 435-453.

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16. Peterson, K. R. 2003. Hemoglobin switching: New insights. Curr. Opin.

Hematol. 10:123-129. 17. Blau, C. A. and K. R. Peterson. 2006. Establishment of cell lines that

exhibit correct ontogenic stage-specific gene expression profiles from tissues of YAC transgenic mice using chemically induced growth signals. In: A. MacKenzie (ed.), Methods in Molecular Biology, Vol. 349: YAC Protocols, 2nd ed., Humana Press, Totowa, N.J., pp. 163-173.

18. Paw, B. H., S.-K. Choe, F. C. Costa, S. V. Sundar, and K. R. Peterson.

2006. Vertebrate Models for Sickle Cell Disease Research. In: B. S. Pace (ed.), Renaissance of Sickle Cell Disease Research in the Genomic Era, World Scientific and Imperial College Press, London, U.K., pp. 237-257.

19. Pace, B. S., S. F. Ofori-Acquah, and K. R. Peterson. 2012. Sickle cell

disease: Genetics, cellular and molecular mechanisms, and therapies. Anemia 2012:e143594. doi:10.1155/2012/143594. PMCID: PMC3432324.

Manuscripts Submitted 1. Debrand, E., L. Chakalova, J. Miles, Y. Dai, B. Goyenechea, S. Dye, C. S.

Osborne, A. Horton, S. Harju-Baker, K. R. Peterson, and P. Fraser. 2012. An intergenic non-coding RNA promoter required for histone modifications in the human beta-globin chromatin domain. PLoS One (Submitted).

Manuscripts in Preparation 1. Peterson, K. R., F. C. Costa, H. Fedosyuk, R. Y. Neades, A. M. Chazelle,

L. Zelenchuk, A. H. Fonteles, P. Dalal, A. Roy, R. Chaguturu, B. Li, and B. S. Pace. 2013. A cell-based high-throughput screen for novel inducers of fetal hemoglobin for treatment of sickle cell disease, Cooley’s anemia and β-thalassemias. Blood (In preparation).

Published Abstracts 1. Mount, D. W., J. W. Little, K. F. Wertman, K. Peterson, G. Lyons, and H.

Ginsberg. 1983. Genetics of the SOS response of E. coli. J. Cell. Biochem. supplement 7B, UCLA Symposia abstracts:163.

2. Peterson, K. R. and D. W. Mount. 1987. Repair of DNA double-strand

breaks in Escherichia coli K-12 DNA adenine methylase (dam-) mutants

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occurs via a recBC-dependent or a recN-dependent pathway. Genetics 116:s38. Annual Meeting of Genetics Society of America, San Francisco, CA.

3. Kidwell, M. G., K. R. Peterson, and S. B. Daniels. 1989. Evolution of P

elements in the genus Drosophila. J. Cell. Biochem., supplement 13C, UCLA Symposia abstracts:88.

4. Peterson, K. R. and G. Stamatoyannopoulos. 1992. The temporal order of

gene expression during development is not determined by the spatial order of genes in a locus. Am. J. Hum. Genet. 51:A54. Presented at Annual Meeting of the American Society of Human Genetics, San Francisco, CA.

5. Peterson, K. R., B. M. Josephson, and G. Stamatoyannopoulos. 1992.

The temporal order of γ and β globin gene expression is independent of their spatial order. Blood 80:243a. Presented at the Annual Meeting of the American Society of Hematology, Anaheim, CA.

6. Zitnik, G., K. Peterson, G. Stamatoyannopoulos, and Th.

Papayannapoulou. 1992. Dexamethasone accelerates switching in human fetal erythroid x MEL hybrids. Blood 80:81a. Annual Meeting of the American Society of Human Genetics, San Francisco, CA.

7. Peterson, K. R., C. H. Clegg, C. Huxley, B. M. Josephson, H. S. Haugen,

T. Furukawa, and G. Stamatoyannopoulos. 1993. Transgenic mice containing a 248 kb human β locus YAC display proper developmental control of human globin genes. Clin. Res. 41:258a. Presented at the Plenary Session of the Annual Meeting of the Association of American Physicians, Washington, D.C.

8. Peterson, K. R., G. Zitnik, C. H. Clegg, A. Gnirke, C. Huxley, K. A. Leppig,

T. Papayannopoulou, and G. Stamatoyannopoulos. 1993. Use of YACs for studies of development: Correct developmental regulation of a 248 kb β globin locus YAC following transfer into mouse erythroleukemia (MEL) cells and into transgenic mice. Am. J. Hum. Genet. 53:A5. Presented at the Plenary Session of the Annual Meeting of the American Society of Human Genetics, New Orleans, LA.

9. Blau, C. A., K. R. Peterson, and G. Stamatoyannopoulos. 1993.

Functional redundancy of silencers in the ε globin promoter. Blood

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82:219a. Annual Meeting of the American Society of Hematology, St. Louis, MO.

10. Furukawa, T., G. Zitnik, K. Leppig, K. Peterson, B. Josephson, Th.

Papayannopoulou, and G. Stamatoyannopoulos. 1993. The LCR can interact with two genes of a single β globin locus. Blood 82:435a. Annual Meeting of the American Society of Hematology, St. Louis, MO.

11. Pace, B., Q. Li, K. Peterson, and G. Stamatoyannopoulos. 1993. On the

mechanism of γ gene induction by butyrate: Role of the LCR and evidence that butyrate cannot reactivate in vivo silenced γ genes. Blood 82:312a. Annual Meeting of the American Society of Hematology, St. Louis, MO.

12. Peterson, K. R., C. H. Clegg, P. A. Navas, T. Furukawa, and G.

Stamatoyannopoulos. 1994. Evidence that YACs can be used for functional studies of regulatory elements of multigenic loci: Production of YAC transgenic mice with -117 Aγ HPFH phenotype. Blood 84:218a. Presented at the Annual Meeting of the American Society of Hematology, Nashville, TN.

13. Peterson, K. R., P. A. Navas, C. H. Clegg, and G. Stamatoyannopoulos.

1995. Use of β-YAC mice for studying LCR function: Effect of 5’ HS 2 or HS 3 deletions on globin gene expression during development. Blood 86:586a. Presented at the Annual Meeting of the American Society of Hematology, Seattle, WA.

14. Zitnik, G., K. R. Peterson, G. Stamatoyannopoulos, and T.

Papayannopoulou. 1995. The silencing of γ-globin gene expression in a β-globin locus YAC can be arrested by α-aminobutyric acid. Blood 86:587a. Annual Meeting of the American Society of Hematology, Seattle, WA.

15. La Spada, A. R., K. Peterson, G. Jeng, K. Chen, K. Fischbeck, and G. S.

McKnight. 1996. Instability of CAG repeat expansions introduced into yeast artificial chromosomes. Am. J. Hum. Genet. 59:A53. Annual Meeting of the American Society of Human Genetics, San Francisco, CA.

16. Blau, C. A., K. R. Peterson, J. G. Drachman, and D. Spencer. 1996. A

drug dependent proliferative switch for genetically modified cells. Blood 88:542a. Annual Meeting of the American Society of Hematology, Orlando, FL.

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17. Navas, P. A., C. H. Clegg, E. Skarpidi, K. R. Peterson, and G.

Stamatoyannopoulos. 1996. β-YAC transgenic mice carrying a deletion of 234 bp core sequence of hypersensitive site 3: Relevance to function of the LCR. Blood 88:462a. Annual Meeting of the American Society of Hematology, Orlando, FL.

18. La Spada, A. R., K. Peterson, M. E. McClain, G. Jeng, S. A. Meadows, K.

Chen, M. Singer, B. Trask, K. H. Fischbeck, and C. H. McKnight. 1997. CAG repeat expansions in androgen receptor YAC transgenic mice are moderately unstable. Am. J. Hum. Genet. 61:A312. Annual Meeting of the American Society of Human Genetics, Baltimore, MD.

19. Navas, P. A., K. R. Peterson, A. Rohde, H. Asano, C. Clegg, E. Skarpidi,

and G. Stamatoyannopoulos. 1997. Transgenic mice with HS3 core deletion: Direct evidence that specific sequences of the LCR have specific developmental roles. Blood 90:274a. Annual Meeting of the American Society of Hematology, San Diego, CA.

20. Papayannopoulou, Th., B. Nakamoto, K. Peterson, and G. Priestley. 1997.

Functional in vivo evidence that an erythroid specific enhancer can be activated in all types of hemopoietic progenitors with extinction later in white cells but consolidation of expression in erythroid and megakaryocytic cells. Blood 90:428a. Annual Meeting of the American Society of Hematology, San Diego, CA.

21. Peterson, K. R., Y. Ji, C. Fernandez-Kodama, and T. Papayannopoulou. 1997.

Deletion of a loxP-flanked human βS gene in erythroid cells following erythroid-specific Cre recombinase expression in transgenic mice. Blood 90:132a. Annual Meeting of the American Society of Hematology, San Diego, CA.

22. Peterson, K. R., C. H. Clegg, P. A. Navas, T. G. Kimbrough, G. M. Alvino,

and G. Stamatoyannopoulos. 1997. Cis-control of globin gene switching resides in gene-specific sequences, rather than gene order. Blood 90:275a. Annual Meeting of the American Society of Hematology, San Diego, CA.

23. Vassilopoulos, G., P. Navas, E. Skarpidi, K. Peterson, and G.

Stamatoyannopoulos. 1997. Normal function of the LCR may require activation in a non erythroid cell environment. Blood 90:274a. Annual Meeting of the American Society of Hematology, San Diego, CA.

24. Jin, L., N. Siritanaratkul, D. W. Emery, R. E. Richard, G. V. Priestley, K. R.

Peterson, K. Kaushansky, T. Papayannopoulou, and C. A. Blau. 1998.

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Reversible immortalization of multipotential, primary hemopoietic cells. Blood Cells, Molecules, & Disease 24:465. Eleventh Conference on Hemoglobin Switching, Orcas Island, WA.

25. Navas, P. A., K. R. Peterson, Q. Li, E. Skarpidi, A. Rohde, S. E. Shaw, C.

H. Clegg, H. Asano, and G. Stamatoyannopoulos. 1998. Transgenic mice with HS3 core deletion: Evidence for developmental specificity of the interaction between the LCR and the embryonic or fetal globin genes. Blood Cells, Molecules, & Disease 24:508. Eleventh Conference on Hemoglobin Switching, Orcas Island, WA.

26. Peterson, K. R., P. A. Navas, C. H. Clegg, T. G. Kimbrough, G. M. Alvino,

A. Rohde, and G. Stamatoyannopoulos. 1998. Cis-control of globin gene switching resides in gene-specific sequences, as well as in gene order. Blood Cells, Molecules, & Disease 24:480-481. Eleventh Conference on Hemoglobin Switching, Orcas Island, WA.

27. Peterson, K. R., P. A. Navas, Q. Li, and G. Stamatoyannopoulos. 1998.

LCR-dependent gene expression in 248 Kb and 155 Kb β-globin YAC transgenics. Blood Cells, Molecules, & Disease 24:511. Eleventh Conference on Hemoglobin Switching, Orcas Island, WA.

28. Sabatino, D. E., K. Peterson, A. P. Cline, L. J. Garrett, P. G. Gallagher, B.

G. Forget, G. Stamatoyannopoulos, and D. M. Bodine. 1998. Substitution of the β-spectrin promoter for the Aγ-globin promoter in transgenic mice in a cosmid construct allows expression of human β-globin mRNA in transgenic mouse yolk sac derived red cells. Blood Cells, Molecules, & Disease 24:513. Eleventh Conference on Hemoglobin Switching, Orcas Island, WA.

29. Vassilopoulos, G., P. A. Navas, E. Skarpidi, K. R. Peterson, C. H. Lowrey,

T. Papayannopoulou, and G. Stamatoyannopoulos. 1998. Correct function of the LCR may require passage through a non-erythroid cellular environment. Blood Cells, Molecules, & Disease 24:519. Eleventh Conference on Hemoglobin Switching, Orcas Island, WA.

30. Sabatino, D. E., K. Peterson, A. P. Cline, L. J. Garrett, P. G. Gallagher,

and D. M. Bodine. 1998. Substitution of the β-spectrin promoter for the Aγ-globin promoter in a YAC construct allows expression of human β-globin in yolk sac derived red cells in transgenic mice. Blood. Annual Meeting of the American Society of Hematology, Miami Beach, FL.

31. Peterson, K. R., P. A. Navas, and G. Stamatoyannopoulos. 1999. Cis-

control of globin gene switching resides in gene-specific sequences, as well

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as in gene order. Blood 94:416a. Annual Meeting of the American Society of Hematology, New Orleans, LA.

32. Peterson, K.R. 2000. Role of gene-specific sequences and gene order in

cis-regulation of globin gene switching. Blood Cells, Molecules & Diseases 26:502. Twelfth Conference on Hemoglobin Switching, Orcas Island, WA.

33. Harju, S., P. A. Navas, G. Stamatoyannopoulos, and K. R. Peterson.

2000. Cis-control of globin gene switching resides in gene-specific sequences, as well as in gene order. Blood Cells, Molecules & Diseases 26:517. Twelfth Conference on Hemoglobin Switching, Orcas Island, WA.

34. Peterson, K. R., S. Harju, P. A. Navas, and G. Stamatoyannopoulos.

2002. Role of γ-globin gene silencing and chromatin sub-domains in globin gene switching. Blood 100:47a. Annual Meeting of the American Society of Hematology, Philadelphia, PN.

35. Peterson, K.R., S. Harju, P. A. Navas, and G. Stamatoyannopoulos. 2003.

Role of γ-globin gene silencing and chromatin sub-domains in globin gene switching. Blood Cells, Molecules & Diseases 31:161. Thirteenth Conference on Hemoglobin Switching, Oxford, UK.

36. Harju, S., P. A. Navas, G. Stamtoyannopoulos, and K. R. Peterson. 2003.

Role of γ-globin gene silencing and chromatin sub-domains in globin gene switching. Blood Cells, Molecules & Diseases 31:145-146. Thirteenth Conference on Hemoglobin Switching, Oxford, UK.

37. Harju, S., K. J. McQueen, H. Fedosyuk, and K. R. Peterson. 2003.

Deletion of LCR 5’HS4 does not markedly affect β-like globin gene expression in β-YAC transgenic mice. Blood Cells, Molecules & Diseases 31:145. Thirteenth Conference on Hemoglobin Switching, Oxford, UK.

38. Peterson, K. R., J. Yan, P. A. Navas, and C. A. Blau. 2003. Establishment

of CID-dependent progenitor cell lines from β-YAC transgenic mice. Blood Cells, Molecules & Diseases 31:161. Thirteenth Conference on Hemoglobin Switching, Oxford, UK.

39. Navas, P. A., R. Swank, K. R. Peterson, and G. Stamatoyannopoulos.

2003. Mutation of a GT motif in the HS3 core element in transgenic mice: Evidence for stage-specific role of GT6 in ε- and γ-globin gene expression during development. Blood Cells, Molecules & Diseases 31:156. Thirteenth Conference on Hemoglobin Switching, Oxford, UK.

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40. Peterson, K. R., J. Yan, P. A. Navas, and C. A. Blau. 2003. γ-globin gene

expression in CID-dependent multi-potential cell lines established from β-YAC transgenic mice. Blood 102:266a. Annual Meeting of the American Society of Hematology, San Diego, CA.

41. Fang, X., J. Sun, G. Barkass, P. A. Navas, K. R. Peterson, G.

Stamatoyannopoulos, and Q. Li. 2003. The 5’HS3 core element of the β-globin LCR is required for formation of hyperacetylated histone domains in the LCR and δ/β-globin gene region during adult erythropoiesis. Blood 102:516a. Annual Meeting of the American Society of Hematology, San Diego, CA.

42. Harju, S., H. Fedosyuk, and K. R. Peterson. 2004. Effect of LCR 5’HS4

and 5’HS1 deletions on β-like globin gene expression in β-YAC transgenic mice. Blood 104:342a. Annual Meeting of the American Society of Hematology, San Diego, CA.

43. Peterson, K. R., H. Fedosyuk, and S. Harju. 2004. LCR 5’HS3 displays

specificity for ε-globin gene activation during primitive erythropoiesis and γ-globin gene activation during fetal definitive erythropoiesis. Blood 104:343a. Annual Meeting of the American Society of Hematology, San Diego, CA.

44. Harju, S. and K. R. Peterson. 2004. In vivo and in vitro analysis of an Aγ-

globin gene silencer in human β-YAC transgenic mice. Blood 104:345a. Annual Meeting of the American Society of Hematology, San Diego, CA.

45. Peterson, K. R., J. Yan, P. A. Navas, C. F. Barbas, III, and C. A. Blau.

2005. γ-globin gene expression in CID-dependent multipotential cells established from β-YAC transgenic mice. Blood Cells, Molecules & Diseases 34:116. Fourteenth Conference on Hemoglobin Switching, Orcas Island, WA.

46. Harju, S., H. Fedosyuk, and K. R. Peterson. 2005. Effect of LCR 5’HS4

and 5’HS1 deletions on β-like globin gene expression in β-YAC transgenic mice. Blood Cells, Molecules & Diseases 34:92. Fourteenth Conference on Hemoglobin Switching, Orcas Island, WA.

47. Harju, S. and K. R. Peterson. 2005. In vivo and in vitro analysis of an Aγ-

globin gene silencer in human β-YAC transgenic mice. Blood Cells, Molecules & Diseases 34:92-93. Fourteenth Conference on Hemoglobin Switching, Orcas Island, WA.

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48. Peterson, K. R., H. Fedosyuk, and S. Harju. 2005. LCR 5’HS3 displays

specificity for ε-globin gene activation during primitive erythropoiesis and γ-globin gene activation during fetal definitive erythropoiesis. Blood Cells, Molecules & Diseases 34:115-116. Fourteenth Conference on Hemoglobin Switching, Orcas Island, WA.

49. Fraser, P., L. Chakalove, J. Miles, D. Carter, A. Horton, E. Debrand, B.

Goyenechea, J. A. Mitchell, C. Kattamis, S. Harju, K. Peterson, and C. S. Osborne. 2005. β-globin locus transcription. Blood Cells, Molecules & Diseases 34:87. Fourteenth Conference on Hemoglobin Switching, Orcas Island, WA.

50. Peterson, K. R., H. Fedosyuk, and S. Harju. 2005. Human β-globin locus

control region hypersensitive site specificity for globin gene activation during erythropoiesis. Blood 106:1010a. Annual Meeting of the American Society of Hematology, Atlanta, GA.

51. Costa, F. C., S. Harju, H. Fedosyuk, L. Zelenchuk, R. Neades, and K. R.

Peterson. 2006. Silencing of Aγ-globin gene expression during adult definitive erythropoiesis is mediated by GATA-1 binding. Blood Cells, Molecules & Disease 38:170-171. Fifteenth Conference on Hemoglobin Switching, Oxford, UK.

52. Peterson, K. R., H. Fedosyuk, and S. Harju. 2006. Human β-globin locus

control region hypersensitive site specificity for globin gene activation during erythropoiesis. Blood Cells, Molecules & Disease 38:170. Fifteenth Conference on Hemoglobin Switching, Oxford, UK.

53. Lavelle, D., V. Banzon, T. Kousnetzova, M. Hankewych, K. R. Peterson,

and J. DeSimone. 2007. RNAi targeting MBD2 increases γ-globin expression in a CID-dependent human β-YAC murine bone marrow cell line. Blood 110:525a. Annual Meeting of the American Society of Hematology, Atlanta, GA.

54. Peterson, K. R., F. C. Costa, and S. Harju-Baker. 2007. Silencing of γ-

globin gene expression during adult definitive erythropoiesis is mediated by a GATA-1 repressor complex. Blood 110:87a. Annual Meeting of the American Society of Hematology, Atlanta, GA.

55. Costa, F. C., H. Fedosyuk, R. Neades, L. Zelenchuk, G. C. Maniscalco, and

K. R. Peterson. 2008. Transactivation of γ-globin gene expression by novel transcription co-factors: TSPYL1, FGIF and MTF-1. Blood Cells, Molecules & Disease, in press. Sixteenth Conference on Hemoglobin Switching, Asilomar, CA.

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56. Costa, F. C., H. Fedosyuk, L. Zelenchuk, and K. R. Peterson. 2008. Modulation of GATA-1 repressor activity at the Aγ-globin-566 GATA silencer during fetal/adult erythropoiesis. Blood Cells, Molecules & Disease, in press. Sixteenth Conference on Hemoglobin Switching, Asilomar, CA.

57. Peterson, K. R., H. Fedosyuk, and F. C. Costa. 2008. Hereditary

persistence of fetal hemoglobin: old, new and future mutations in the Aγ-globin gene-proximal region. Blood 112:492a. Annual Meeting of the American Society of Hematology, San Francisco, CA.

58. Ibanez, V., V. Banzon, T. Kousnetzova, K. Vaitkus, K. Peterson, J.

DeSimone, and D. Lavelle. 2008. The role of DNA damage/stress response pathways in the mechanism of action of decitabine. Blood 112:490a. Annual Meeting of the American Society of Hematology, San Francisco, CA.

59. Stavrou, E. F., E. Lagadinou, E. Papapetrou, N. Zoumbos, C. Barbas III, K.

Peterson, and A. Athanassiadou. 2008. S/MAR based episomal vector mediates induction of gamma-globin expression by a specific zinc-finger activator in K562 and murine progenitor cells. In Press. European Hematology Association Congress, Copenhagen, Denmark.

60. Stavrou, E. F., E. Lagadinou, E. Papapetrou, N. Zoumbos, C. Barbas III, K.

Peterson, and A. Athanassiadou. 2008. S/MAR based episomal vector mediates induction of gamma-globin expression by a specific zinc-finger activator in K562 and murine progenitor cells. Human Gene Therapy 19:1113. European Society of Gene and Cell Therapy Annual Congress, Brugge, Belgium.

61. Costa, F. C., H. Fedosyuk, and K. R. Peterson. 2009. The -566 Aγ-globin HPFH mutation disrupts temporal repression of fetal hemoglobin synthesis during fetal liver definitive erythropoiesis. Blood 114:797. Annual Meeting of the American Society of Hematology, New Orleans, LA. Also published on-line in F1000 Poster Bank (http://posters.f1000.com/).

62. Banzon, B., V. Ibanez, K. Vaitkus, K. Peterson, J. DeSimone, and D. Lavelle. 2009. siRNA targeting DNA Methyltransferase increases ε- and γ-globin expression. Blood 114:402. Annual Meeting of the American Society of Hematology, New Orleans, LA.

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63. Stavrou, E. F., E. Lagadinou, N. Zoumbos, A. Spyridonidis, C. Barbas III, K.

Peterson, and A. Athanassiadou. 2010. Development of an S/MAR based episomal vector of a specific zinc-finger activator that mediates gamma-globin gene activation, for the gene therapy of hemoglobinopathies. Molecular Therapy 18:S132. Annual Meeting of the American Society of Gene and Cell Therapy, Washington, DC.

64. White, R. A., D. P. Heruth, T. Hawkins, D. Logsdon, M. Gibson, I.

Sokolovsky, N. Nsumu, S. Major, B. Fegley, G. Woods, K. Lewing, K. Neville, K. Cornetta, and K. Peterson. 2010. Mutation in erythroid specific transcription factor KLF1 causes hereditary spherocytosis in the Nan (neonatal anemia) hemolytic anemia mouse model. Blood 116:1320. Annual Meeting of the American Society of Hematology, Orlando, FL.

65. Peterson, K. R., F. C. Costa, H. Fedosyuk, R. Neades, J. Bravo de los

Rios, A. Fonteles, L. Zelenchuk, P. Dalal, and G. C. Maniscalco. 2011. Induction of fetal hemoglobin by transcriptional co-activators MTF-1 and TSPYL1. Blood (In press). Annual Meeting of the American Society of Hematology, San Diego, CA.

Electronic submissions 1. McQueen, K. J. and K. R. Peterson. 2001. Mus musculus polycystin 1

(Pkd1) gene, exons 39 through 46 and partial cds; and tuberous sclerosis 2 (Tcs2) gene, partial cds. GenBank AF333927.

INVITED TALKS 1992 DNX Symposium on Hemoglobin, Princeton, NJ. Regulation of

hemoglobin switching in transgenic mice. 1992 Eighth Hemoglobin Switching Conference, Orcas Island, WA. Importance

of spatial order of γ and β globin genes in temporal order of expression. 1994 Gladstone Institute of Cardiovascular Disease, University of California San

Francisco, San Francisco, CA. Use of yeast artificial chromosomes (YACs) in the production of transgenic mice.

1994 GenPharm International, Mountain View, CA. Generation of transgenic mice using YACs.

1994 Ninth Conference on Hemoglobin Switching, Orcas Island, WA. Transgenic Mice Using β Locus YACs.

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1995 Gordon Conference on Red Cells, Plymouth, NH. Production of β-globin

locus YAC transgenic mice carrying human globin developmental mutants.

1996 Engineered Animal Models, Baltimore, MD. Production of β-globin locus YAC transgenic mice carrying human globin developmental mutants.

1996 Tenth Conference on Hemoglobin Switching, Orcas Island, WA. Use of YACs for studies of switching.

1997 Seventh Cooley’s Anemia Symposium, Cambridge, MA. β-YAC transgenic mice for studying LCR function.

1998 Keystone Symposium on Genomics and Drug Discovery, Santa Fe, NM. Analysis of developmental regulation in YAC transgenic mice.

1998 Distinguished Scientist Seminar, University of South Alabama, Mobile, AL. Analysis of human β-globin gene regulation in YAC transgenic mice.

1998 Eleventh Conference on Hemoglobin Switching, Orcas Island, WA. Cis-control of globin gene switching resides in gene-specific sequences, rather than gene order.

1998 Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS. Cis-control of globin gene switching.

1999 Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS. Role of cis-linked sequences and locus organization in regulation of β-globin gene expression.

1999 Department of Veterans Affairs Medical Center, Kansas City, MO. Developmental regulation of β-globin gene expression.

2000 School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO. Developmental regulation of β-globin gene expression: Role of cis-linked sequences and locus organization.

2000 Twelfth Conference on Hemoglobin Switching, Orcas Island, WA. Role of gene-specific sequences and gene order in cis-regulation of globin gene switching.

2001 Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, KS. Locus-linked regulatory motifs of human β-globin gene switching.

2002 Thirteenth Conference on Hemoglobin Switching, Oxford, UK. Role of γ-globin gene silencing and chromatin sub-domains in globin gene switching.

2002 Stowers Institute for Medical Research, Kansas City, MO. Molecular control of β-globin gene switching.

2003 Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS. Long-range regulation of globin gene expression by the locus control region.

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2003 Division of Genetic Medicine, Vanderbilt University, Nashville, TN.

Molecular control of human β-globin gene switching. 2003 Department of Biology, University of Louisiana at Lafayette, Lafayette, LA.

Molecular control of human β-globin gene switching. 2003 Investigator Research Award, University of Kansas Medical Center,

Kansas City, KS. Long-range activation of β-globin gene expression by the locus control region.

2004 Fourteenth Conference on Hemoglobin Switching, Orcas Island, WA. LCR HS Specificity for globin gene activation.

2004 Institute of Biomedical Sciences and Technology, University of Texas at Dallas, Richardson, TX. Cis-acting determinants of γ-globin gene activation.

2005 Greater Kansas City Association of Family & Consumer Sciences and Business Professionals in Family & Consumer Sciences, Kansas City, MO. Science and ethics: The intertwined debate on stem cells using technology to enhance quality of life.

2005 Division of Genetic Medicine, Vanderbilt University, Nashville, TN. Molecular control of fetal globin gene expression.

2005 Kansas City Metropolitan Bar Association Annual Bench-Bar and Boardroom Conference, Lake of the Ozarks, MO. The embryonic stem cell research debate – conflicts in law, science and ethics.

2005 Region Eight Conference of the American Medical Student Association, Kansas City, MO. The embryonic stem cell research debate – conflicts in science, medicine, ethics, law, and public policy.

2006 Fifteenth Conference on Hemoglobin Switching, Oxford, UK. Silencing of Aγ-globin gene expression during adult definitive erythropoiesis is mediated by GATA-1 binding.

2007 Transcription, Chromatin and Cancer, Stowers Institute for Medical Research, Kansas City, MO. GATA-1-mediated silencing of fetal hemoglobin expression.

2008 Sixteenth Conference on Hemoglobin Switching, Asilomar, CA. Transactivation of γ-globin gene expression by novel transcription co-factors: TSPYL1, FGIF AND MTF-1.

2008 Intercultural Center, Kansas City Kansas Community College, Kansas City, KS. Human stem cells.

2009 Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS. Transactivation and repression of fetal hemoglobin expression: a bimodal approach to treat sickle cell disease.

265

2009 Department of Molecular and Cell Biology, University of Texas at Dallas,

Richardson, TX. Transactivation and repression of fetal hemoglobin expression: a bimodal approach to treat sickle cell disease.

2010 Department of Biological Sciences, Kansas City Kansas Community College, Kansas City, KS. Human Stem Cell Research.

2010 Department of Biology, University of Louisiana at Lafayette, Lafayette, LA. Activation of silent genes for the treatment of disease: A paradigm from globin gene switching.

2010 Seventeenth Conference on Hemoglobin Switching, Oxford, UK. Induction of fetal hemoglobin by MTF-1.

2011 Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS. Transcriptional activation of γ-globin: The therapeutic approach for treatment of sickle cell disease.

2012 Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL. Fetal hemoglobin: regulation, activation and treatment for sickle cell disease.

TEACHING ACTIVITIES (49 contact hours total) Current Graduate Student Courses GSMC 850, Proteins and Metabolism (2009-present), 6 contact hours IGPBS 898, Introduction to Faculty Research (1998-present), 1 contact hour BCHM 922, Advanced Molecular Genetics (2000-present), Course Coordinator

(2012), 17 contact hours PATH 803, Stem Cell Biology, (2005-present), 2 contact hours PATH 805, Epigenetics (2009-present), 3 contact hours Current Medical Student Courses Module 1, Foundations of Medicine, Small Group Author (2006-present), 2

contact hours Module 1, Foundations of Medicine, Small Group Discussion (2006-present), 8

contact hours Module 2, Genetics and Neoplasia, Lecture (2006-present), 4 contact hours Module 2, Genetics and Neoplasia, Small Group Discussion (2006-present), 4

contact hours 266

Module 2, Genetics and Neoplasia, Homework Discussion (2006-present), 1

contact hour Module 2, Genetics and Neoplasia, Review session (2006-present), 1 contact

hour Previous Graduate Student Courses BCHM 862, Literature Seminar (1999-2012), Course Coordinator, 10 contact

hours BCHM 893, Module 3 of the IGPBS: Molecular Biology (2000-2008), 13 contact

hours; Co-Director of the course (2008) BCHM 894, Module 4 of the IGPBS: Cell and Developmental Biology (2000-

2005), 4 contact hours BCHM 894, Module 4 of the IGPBS: Cell and Developmental Biology (2007-

2008), 2 contact hours Previous Medical Student Courses BIOC 801/802 Medical Biochemistry Small Group Discussion (1999-2006), 21

contact hours Medical Biochemistry 801/Medical Physiology 801/Introduction to Clinical

Medicine 801 PBL (2005), 4 contact hours (2 listed in Small Group Discussion)

BIOC 802 Medical Biochemistry (1999-2006), 3 contact hours Module 2, Genetics and Neoplasia, Large Group Discussion (2006-2011), 1

contact hour Genetics lecture, Surgical Residents (2008), 1 contact hour FELLOWS & STUDENTS DIRECTED/MENTORING

Postdoctoral Trainees

Patrick A. Navas, Division of Medical Genetics, University of Washington, 1993-1998.

267

Yuanen Ji, Division of Medical Genetics, University of Washington, 1995-1997.

Susan L. Harlocker, Division of Medical Genetics, University of Washington, 1998.

Auste Geddes, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2001-2003. Biomedical Research Training Program Postdoctoral Scholar, 2003.

Rita Lee, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2002-2003.

Susanna Harju, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2003-2004.

Shirin V. Sundar, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2005-2006.

Flávia C. Costa, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2005-2012. Biomedical Research Training Program Postdoctoral Scholar, 2006-2008.

Doctoral Graduate Students

Susanna Harju, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 1998-2003. Ph.D., 10/03/03. Biomedical Research Training Program Predoctoral Scholar.

Kellie J. McQueen, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 1999-2001. Biomedical Research Training Program Predoctoral Scholar.

Rachel L. Grau, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2009-2010.

Allen Chazelle, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2012-present.

Masters Graduate Students

Anna Nunn, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2002-2007. M.S., 04/23/07.

268

Rotation Students

Yi Yang, Department of Genetics, University of Washington, 1997.

Huimin Jiang, Interdisciplinary Graduate Program in Biomedical Sciences (IGPBS), University of Kansas Medical Center, 2nd rotation, 1998-1999.

Kellie J. McQueen, IGPBS, University of Kansas Medical Center, 2nd rotation, 1998-1999.

Greg J. Bartelma, IGPBS, University of Kansas Medical Center, 3rd rotation, 1999-2000.

S. Joshua Langmade, IGPBS, University of Kansas Medical Center, 3rd rotation, 1999-2000.

Irma Gresshoff, IGPBS, University of Kansas Medical Center, 1st rotation, 2000-2001.

Christina Newport, IGPBS, University of Kansas Medical Center, 1st rotation, 2001-2002.

Anna Nunn, IGPBS, University of Kansas Medical Center, 2nd rotation, 2001-2002.

Kara Wagoner, IGPBS, University of Kansas Medical Center, 2nd rotation, 2002-2003.

Raymond Camahort, IGPBS, University of Kansas Medical Center, 2nd rotation, 2003-2004.

Andrew Ralya, IGPBS, University of Kansas Medical Center, 1st rotation, 2004-2005.

Nathan Bushue, IGPBS, University of Kansas Medical Center, 4th rotation, Fall 2011; co-mentored with Dr. Chad Slawson.

Allen Chazelle, IGPBS, University of Kansas Medical Center, 1st rotation, Fall 2011.

Nancy Stiles, University of Kansas Medical Center, 3rd rotation, Spring 2012.

Medical Student Trainees

Joel D. Ackerman, Summer Research Traineeship for Medical Students, University of Kansas Medical Center, 2000.

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James M. Howard, Summer Research Traineeship for Medical Students, University of Kansas Medical Center, 2001; Winner, Santiago Grisolia Award for Excellence in Biochemistry Research by a Medical Student, 2002.

Bennett Berning, Summer Research Traineeship for Medical Students, University of Kansas Medical Center, 2012.

High School Students

David Nicholl, Mentorship Academy Program, Blue Valley School District, Overland Park, KS, 1999-2000.

Parmita Dalal, Science Fair Project, Shawnee Mission Northwest High School, Lenexa, KS, 2000-2002; Winner, Grand Award (and five other awards), 51st Greater Kansas City Science and Engineering Fair, 2002; Fourth place (of 65) in Biology Division of International Science Fair, 2002.

Maria Martinez, Volunteer, 2003.

Taras Zelenchuk, Volunteer and Employee, Shawnee Mission East High School, Prairie Village, KS, 2003-2004.

Patrick Sturdivant, Volunteer and Employee, Olathe East High School, Olathe, KS, 2004.

Ali Heitmann, Employee, Liberty High School, Liberty, MO, 2004-2005.

Gabriella Maniscalco, Volunteer Internship and Employee, Oak Park High School, Kansas City, MO, 2006-2008; Awarded Gold Medallion Diploma and Academic Honors.

Prarthana Dalal, Science Fair Project, Shawnee Mission East High School, Prairie Village, KS, 2008-2011; First Place, Kansas-Nebraska-Oklahoma Regional Junior Science and Humanities Symposium, 2009, 2010, 2011; Winner, Grand Award (and two other awards), 58th Greater Kansas City Science and Engineering Fair, 2009; Winner, Grand Award (and six other awards), 59th Greater Kansas City Science and Engineering Fair, 2010; Technology Showcase Award, Award of Distinction, Vic Regnier Award, Research and Development Forum, 2010; Special Award – AVASC Foundation, Fourth place – Medicine and Health Sciences Category, International Science and Engineering Fair, 2010; Regional Finalist, Siemens Competition, 2010; Winner, Grand Award (and two other awards), 60th Greater Kansas City Science and Engineering Fair, 2011; First Place, Junior Science and Humanities Symposium, 2011; Vic Regnier Award, Shawnee Mission School District Research and Development Forum, 2011; Third place Grand Award – Medical Research Category, Intel International Science and Engineering Fair, 2011; First place, International BioGENEius Challenge, 2011.

Yuliya Matskevych, Employee, Shawnee Mission Northwest High School, Shawnee Mission, KS, 2010-12.

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Emily Binshtok, Volunteer, Blue Valley West High School, Overland Park, KS, 2011.

Gaspar Maisonet, Volunteer, Olathe Northwest High School, Overland Park, KS, 2012.

University Undergraduate Students

Hanluen Kao, Employee, University of Kansas, Lawrence, KS, 2003.

Taras Zelenchuk, Employee, Shawnee Mission East High School, Prairie Village, KS and University of Kansas, Lawrence, KS, 2005-2006.

Patrick Sturdivant, Employee, University of Kansas, Lawrence, KS, 2005-2006; Recipient of University of Kansas Cancer Center Summer Student Research Training Program award, 2007.

Kelsey Simpson, Employee, University of Kansas, Lawrence, KS, 2008.

Kayleigh Peterson, Employee, University of Kansas, Lawrence, KS, 2008-2010.

Gabriella Maniscalco, Employee, St. Louis University, St. Louis, MO, 2009.

Johana, Bravo de los Rios, Employee, University of Kansas, Lawrence, KS, 2010.

Other

Julia Draper, Volunteer, Graduate of Rhodes College, Memphis, TN, 2011-2012.

Tyler J. Stephenson, Kidney Institute-funded, Laboratory-based Summer Research Position, Graduate of Baker University, Baldwin City, KS, 2012.

Matt Parker, Graduate of University of Kansas, Lawrence, KS, 2013.

Dean’s Scholars Faculty Mentor

Trinh Le (Amy) Doan, University of Kansas Honors Program, Lawrence, KS, 2004-2006.

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Visiting Scholars

Tiago de Andrade, Graduate Student, Molecular Biology and Gene Therapy Laboratory, HEMOCENTRO (UNICAMP), Cidade Universitária Zeferino Vaz, Campinas, Brazil, 2004. Luciana Sarmento Moreira, Graduate Student, Molecular Biology and Gene Therapy Laboratory, HEMOCENTRO (UNICAMP), Cidade Universitária Zeferino Vaz, Campinas, Brazil, 2004.

Mentored Junior Faculty

Grace L. Guo, Ph.D., Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, 2004-2008.

Aron W. Fenton, Ph.D., Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2004-present.

Liskin Swint-Kruse, Ph.D., Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2004-2009.

Jay L. Vivian, Ph.D., Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, 2005-2006.

Mukta Sharma, M.D., M.P.H., Department of Pediatrics, University of Kansas Medical Center, 2006-2009; Mentor, NHLBI Summer Institute Program to Increase Diversity (SIPID) – Red Blood Cell Disorders, University of Texas at Dallas, 2007-2009; Primary Mentor, Department of Pediatrics Faculty Mentoring Program, University of Kansas Medical Center, 2007-2008.

Patrick E. Fields, Ph.D., Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, 2007-2011.

Partha Krishnamurthy, Ph.D., Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, 2007-2011.

Xiaobo Zhong, Ph.D., Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, 2008-2010.

Valerie Schroeder, M.D., Department of Pediatric Cardiology, University of Kansas Medical Center, 2009.

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Jyoti Panicker, M.D., Department of Pediatric Hematology/Oncology, University of Kansas Medical Center, 2011-present.

Stephen Parnell, Ph.D. Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2011-present.

Levi H. C. Makala, D.V.M., M.B.A., Ph.D., Department of Pediatrics, Hematology/Oncology Section, Georgia Health Sciences University; PRIDE Summer Institute Programs to Increase Diversity among Individuals Engaged in Health-Related Research – Functional and Applied Genomics of Blood Disorders, Georgia Health Sciences University, 2011-present.

Omar Aljitawi, M.D., Division of Hematology/Oncology, Department of Internal Medicine, University of Kansas Medical Center, 2012-present.

Comprehensive Exam Committees

Student Department Mentor Date

Susanna Harju Biochem./Mol. Biol. Kenneth R. Peterson 06/01/99

Huimin Jiang Biochem./Mol. Biol. Glen K. Andrews 05/08/00

Kellie J. McQueen Biochem./Mol. Biol. Kenneth R. Peterson 07/19/00

Julie A. Carlsten Anatomy/Cell Biol. Douglas E. Wright 10/24/00

Ryan Thummel Molec./Integr. Physiol. Alan R. Godwin 11/30/01

Huaijin Zhou Micro./Mol. Genet./Immun. Joe Lutkenhaus 01/17/02

Brian P. Hermann Molec./Integr. Physiol. Leslie L. Heckert 02/27/02

Jing Liu Stowers Institute/ Arcady Mushegian 05/16/03

Micro./Mol. Genet./Immun.

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Peizhen Song Molec./Integr. Physiol. Alan R. Godwin Withdrew

Benjamin Weaver Biochem./Mol. Biol. Glen K. Andrews 04/19/05

Rachel Buckley Stowers Institute/ Robb Krumlauf 04/21/05

Anatomy/Cell Biol.

Matthew Goering Stowers Institute/ Jennifer Gerton 05/17/05

Biochem./Mol. Biol.

Laura Galinañes-García Stowers Institute/ Robb Krumlauf Withdrew

Anatomy/Cell Biol.

Raymond Camahort Stowers Institute/ Jennifer Gerton 02/27/06

Biochem./Mol. Biol.

Aaron Gottschalk Stowers Institute/ Joan Conaway 05/26/06

Biochem./Mol. Biol.

Bang Shen Micro./Mol. Genet./Immun. Joe Lutkenhaus 11/07/07

Subhashchandra Naik Biochem./Mol. Biol. Mark T. Fisher 05/08/09

Lu Chen Stowers Institute/ Joan Conaway 08/20/09

Biochem./Mol. Biol.

Shuai Lu Stowers Institute/ Jennifer Gerton 11/03/10

Biochem./Mol. Biol.

Valentine Agbor Molec./Integr. Physiol. Leslie L. Heckert 11/08/10

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Yi Feng Pathology/Lab. Med. Patrick E. Fields 11/15/10

Elizabeth Dille Molec./Integr. Physiol. Leslie L. Heckert 12/09/10

Mauricio Vargas Uribe Biochem./Mol. Biol. Alexey S. Ladokhin 12/06/11

Julie Mitchell Micro./Mol. Genet./Immun. Thomas Yankee 03/19/12

Rushi Trivedi Stowers Institute/ Joan Conaway 05/11/12

Biochem./Mol. Biol.

Allen Chazelle Biochem./Mol. Biol. Kenneth R. Peterson

Zhen Zhang Biochem./Mol. Biol. Chad Slawson

Nairita Roy Molec./Integr. Physiol. Russell Swerdlow

Nehemiah Alvarez Pathology/Lab. Med. Patrick E. Fields

Dissertation/Thesis Committees

Student Department Mentor Dates

Susanna Harju Biochem./Mol. Biol. Kenneth R. Peterson 10/01/98-10/03/03

Kellie J. McQueen Biochem./Mol. Biol. Kenneth R. Peterson 01/15/99-06/30/01

Huimin Jiang Biochem./Mol. Biol. Glen K. Andrews 02/19/01-04/02/04

S. Joshua Langmade Biochem./Mol. Biol. (M.S.) Glen K. Andrews 03/07/01-11/01/01

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Huaijin Zhou Micro./Mol. Genet./Immun. Joe Lutkenhaus 04/22/02-12/08/04

Anna Nunn Biochem./Mol. Biol. (M.S.) Kenneth R. Peterson 06/01/02-04/23/07

Ryan Thummel Molec./Integr. Physiol. Alan R. Godwin 06/24/02-06/25/04

Xinghao Wang Stowers Institute/ Robb Krumlauf 08/12/02-08/01/03

Anatomy/Cell Biol. (M.S.)

Brian P. Hermann Molec./Integr. Physiol. Leslie L. Heckert 02/24/03-10/04/05

Jing Liu Stowers Institute/ Arcady Mushegian 05/16/03-04/18/05

Micro./Mol. Genet./Immun.

Dahzi Liu Stowers Institute/ Ting Xie 05/24/04-12/05/05

Anatomy/Cell Biol. (M.S.)

Benjamin Weaver Biochem./Mol. Biol. Glen K. Andrews 04/19/05-04/03/09

Rachel Buckley Stowers Institute/ Robb Krumlauf 04/21/05-05/23/08

Anatomy/Cell Biol.

Matthew Goering Stowers Institute/ Jennifer Gerton 05/17/05-12/03/10

Biochem./Mol. Biol.

Daniel Kirilly Stowers Institute/ Ting Xie 11/22/05-07/19/06

Anatomy/Cell Biol.

Raymond Camahort Stowers Institute/ Jennifer Gerton 02/27/06-12/14/08

Biochem./Mol. Biol.

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Aaron Gottschalk Stowers Institute/ Joan Conaway 05/26/06-05/27/10

Biochem./Mol. Biol.

Elizabeth Dille Molec./Integr. Physiol. Leslie L. Heckert 08/07/07-present

Bang Shen Micro./Mol. Genet./Immun. Joe Lutkenhaus 09/06/07-04/01/10

Subhashchandra Naik Biochem./Mol. Biol. Mark T. Fisher 03/31/08-11/29/12

Yi Feng Pathology/Lab. Med. Patrick E. Fields 11/12/08-09/18/12

Lu Chen Stowers Institute/ Joan Conaway 04/15/09-present

Biochem./Mol. Biol.

Valentine Agbor Molec./Integr. Physiol. Leslie L. Heckert 04/17/09-08/31/12

Shuai Lu Stowers Institute/ Jennifer Gerton 10/05/09-present

Biochem./Mol. Biol.

Mauricio Vargas Uribe Biochem./Mol. Biol. Alexey S. Ladokhin 01/05/11-present

Rushi Trivedi Stowers Institute/ Joan Conaway 08/30/11-present

Biochem./Mol. Biol.

Julie Mitchell Micro./Mol. Genet./Immun. Thomas Yankee 10/03/11-present

Nairita Roy Pathology/Lab. Med. Soumen Paul 10/19/11-04/02/12

Molec./Integr. Physiol. Russell Swerdlow 08/28/12-present

Allen Chazelle Biochem./Mol. Biol. Kenneth R. Peterson 05/11/12-present

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Zhen Zhang Biochem./Mol. Biol. Chad Slawson 08/09/12-present

Nehemiah Alvarez Pathology/Lab. Med. Patrick E. Fields 02/11/13-present

International Dissertation/Thesis Examination Committees

2011 Yuen Tien Tan, PhD. dissertation, Mentor: Professor S. M. Jane, Department of Medicine (RMH), University of Melbourne, Victoria, Australia

2013 Fiona Claire, Ph.D. dissertation, Co-mentors: Professor S. M. Jane and Associate Professor D. J. Curtis, Department of Medicine (RMH), University of Melbourne, Victoria, Australia

Educational Materials Development

Web materials for BIOC 802 lectures on developmental regulation, animal

models, gene therapy, and human stem cells, 2000-2006. Syllabus for BCHM 893, IGPBS Module 3 lectures on RNA splicing, 2000-2008. Syllabus for BCHM 894, IGPBS Module 4 lectures on gene regulation, 2001-

2005. Development and implementation of new advanced graduate curriculum,

Department of Biochemistry and Molecular Biology, 2003-2004. Development and implementation of new BCHM 893, IGPBS Module 3

curriculum, 2004. Syllabus for BCHM 893, IGPBS Module 3 lectures on DNA replication and repair,

2004-2008. Sickle Cell Disease Small Group Discussion unit, Module 1, Foundations of

Medicine, 2006-2012. Web materials for Module 2, Genetics and Neoplasia lectures and large group

discussions, 2006-2012. Lectures and Laboratory Methods, NHLBI Summer Institute Program to Increase

Diversity (SIPID) – Red Blood Cell Disorders, University of Texas at Dallas, 2007-2010.

Course schedule and paper discussion guidelines, BCHM 893, IGPBS Module 3, 2008.

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Syllabus for GSMC 850, Proteins and Metabolism lectures on regulation of

chromatin structure, structure of chromosomes, genome evolution and student literature discussion, 2009-2012.

Lectures and Laboratory Methods, PRIDE Summer Institute Programs to Increase Diversity among Individuals Engaged in Health-Related Research, Georgia Health Sciences University, 2011-2012. Lecture, Center for Advanced Professional Studies (CAPS), Blue Valley School District, Overland Park, KS, 2012-2013.

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Curriculum Vitae

Chad Slawson

Department of Biochemistry and Molecular Biology Office: (913) 945-6468

University of Kansas School of Medicine Fax: (913) 588-9896

3901 Rainbow Blvd. MS3030

Kansas City, KS 66160 Email: cslawson@kumc.edu

u Education

2007 Postdoctoral fellow in the laboratory of Dr. Gerald W. Hart, Department of Biological Chemistry, The Johns Hopkins School of Medicine

2002 Ph.D. University of South Florida, Department of Chemistry

Chemistry Mentor: Robert Potter • Dissertation Title: Characterization of Novel Single Sugar Protein

Modifications in Proliferative Systems

1994 B.S. Indiana University

Biochemistry

Professional Experience

• November 2012 to present: Associate Member, Cancer Biology, The University of

Kansas Cancer Center • December 2011 to present: Member Institute for Reproductive Health and Regenerative

Medicine, The University of Kansas Medical Center • January 2011 to present: Assistant Professor in the Department of Biochemistry and

Molecular Biology, The University of Kansas Medical Center • August 2011 to June 2012: Consultant - Mediomics, LLC. St. Louis, MO • September 2007 to 2010: Research Associate in the laboratory of Gerald W. Hart,

Department of Biological Chemistry, The Johns Hopkins School of Medicine

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Awards and Honors

• Best Thematic Poster: DNA Replication, Recombination, Repair presented by the JBC at

the 2012 Experimental Biology Meeting • Department of Biological Chemistry representative to Post-Doctorial Council, The Johns

Hopkins School of Medicine, Fall 2005 • American Society for Biochemistry and Molecular Biology Travel Award, June 2004 • Conference Chair, 1st Annual Raymond N. Castle Student Research Conference,

Department of Chemistry, University of South Florida, April 28, 2001 • Graduate Representative to the Department of Chemistry, University of South Florida,

August 2000 – 2001 • Tharpe Travel Award: Department of Chemistry, University of South Florida, November

2000 • Tharpe Summer Fellowship: Department of Chemistry, University of South Florida, May

2000 • Provost Teaching Award Honorable Mention: University of South Florida, April 2000 • Tharpe Teaching Award: Department of Chemistry, University of South Florida, May 1998 • Provost Teaching Award Nominee: University of South Florida, April 1998 and 1999

Professional Affiliation

• American Heart Association – Member, 2013 • American Society for the Advancement of Science – Member, 2011 • Society for Glycobiology – Member, 2008 • American Chemical Society – Member, 2007 • American Society for Biochemistry and Molecular Biology – Member, 2006

Publications

1. Tan, E., Caro, S., Potnis, A., Lanza, C., and Slawson, C. (2013) O-GlcNAc cycling regulates mitotic spindle organization. Sci. Signal (submitted).

2. McGreal, S., Bhushan, B., Walesky, C., Cleveland, J., Gallagher, T., Zhang, Z., Slawson, C., and Apte, A. (2013) Increased Hepatic O-GlcNAcylation Aggravates Acetaminophen-induced Liver injury. Toxicological Sciences (In Review).

3. Gao, X., Wang, X., Pham, T.H., Feuerbacher, L.A., Lubos, M.L., Huang, M., Olsen, R., Mushegian, A., Slawson, C., and Hardwidge, P.R. (2013) NleB, a bacterial effector with glycosyltransferase activity, targets GAPDH function to inhibit NF-κB activation. Cell Host & Microbe 13:1-13. PMC3553500

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4. Slawson, C., and Hart, GW. (2011) O-GlcNAc signaling: implications for cancer cell biology. Nat. Rev. Cancer, 11: 678-684.

5. Hart, G.W., Slawson, C., Ramirez-Correa, G., and Lagerlof, O. (2011) Crosstalk Between O-GlcNAcylation and Phosphorylation: Roles in Signaling, Transcription, and Chronic Disease. Annual Reviews in Biochemistry, 80: 825-858.

6. Wells, L.*, Slawson, C.*, and Hart, G.W. (2011) The E2F-1 Associated

Retinoblastoma-Susceptibility Gene Product is Modified by O-GlcNAc. Amino Acids 40:877-883. * Each author contributed equally to the manuscript.

7. Slawson, C., Copeland, R.J., and Hart, G.W. (2010) O-GlcNAc Siganling:

A Metabolic Link Between Diabetes and Cancer? Trends in Biochem. Sci. 35:547-555.

8. Wang, Z.*, Udeshi, N.D.*, Slawson, C.*, Compton, P.D., Sakabe, K.,

Cheung, W.D., Shabanowitz, J., Hunt, D.F., and Hart G.W. (2010) O-GlcNAcylation Regulates Mitotic Spindle/Midbody Phosphorylation. Sci Signal 3, ra2. * Each author contributed equally to the manuscript. Featured in a Science Signaling perspective, M. C. Hall, Proteomics Modifies Our Understanding of Cell Cycle Complexity. Sci. Signal. 3, pe4 (2010).

9. Li, X., Molina, H., Huang, H., Zhang, Y-Y., Liu, M., Qian, S-W., Slawson,

C., Dias, W.B., Pandey, A., Hart, G.W., Lane, M.D., and Tang, Q-Q. (2009) O-Linked N-acetylglucosamine modification on C/EBPβ: role during adipocyte differentiation. J. Biol. Chem. 284:19248-19254.

10. Slawson, C. Lakshmanan, T., Knapp, S., and Hart, G.W. (2008) A mitotic

GlcNAcylation/Phosphorylation signaling complex alters the post-translational state of the cytoskeletal protein vimentin. Mol. Biol. Cell 19:4130-4140. Selected for cover and previewed in ASCB InCyctes.

11. Hart, G.W., Housley, M.P., and Slawson, C. (2007) Cycling of O-Linked β-

N-acetylglucosamine on nucleocytoplasmic proteins. Nature 446:1017-1022.

12. Slawson, C., Housley, M.P., and Hart, G.W. (2006) O-GlcNAc cycling:

how a single sugar post-translational modification is changing how we think about signaling networks. J. Cell. Biochem. 97:71-83. Selected for cover.

13. Slawson, C., Zachara, N.E., Vosseller, K., Cheung, W.D., Lane, M.D., and

Hart, G.W. (2005) Perturbations in O-linked β-N-acetylglucosamine protein

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modification cause severe defects in mitotic progression and cytokinesis. J. Biol. Chem. 280:32944-32956.

14. Slawson, C., and Hart, G.W. (2003) Dynamic interplay between O-GlcNAc

and O-Phosphate: the sweet side of protein regulation. Curr. Opin. Struct. Biol. 12:631-636.

15. Slawson, C., Shafii, S., Amburgey, J., and Potter, R.L. (2002)

Characterization of the O-GlcNAc protein modification in Xenopus laevis oocytes during oogenesis and progesterone stimulated maturation. Biochim. Biophys. Acta 1573:121-129.

16. Slawson, C., Pidala, J., and Potter, R.L. (2001) Increased N-Acetyl-β-

glucosaminidase activity in primary breast carcinomas corresponds to a decrease in N-acetylglucosamine containing proteins. Biochim Biophys Acta 1537:147-57.

17. Slawson, C., Stewart, J., and Potter, R.L. (2001) Biocatalytic lactone

generation in genetically engineered E. coli and identification of products by gas chromatography – mass spectroscopy. J. Chem. Edu. 78:1533-1544.

Research Funding Ongoing Research Support:

1. P20 RR024214-04 (D. Abrahamson) Molecular Regulation of Cell Development and

Differentiation

This application establishes a multidisciplinary Center for Biomedical Research Excellence (COBRE) with a developmental biology emphasis. Funds are requested to support 3 scientific core facilities and an Administrative Core, 5 research projects, and provide for a strong junior faculty recruiting and mentoring program. There are no funds in this grant that provide research support for the Abrahamson laboratory.

Chad Slawson receives $147,000 (for up to 3 years) with 50% effort on the project “Targeting and Regulation of O-GlcNAc Transferase at M Phase”.

2. P30AG035982 (R. Swedlow)

NIH/NIA

This application established the University of Kansas School of Medicine Alzheimer’s Center. The goal of the KUADC is to support Alzheimer’s and brain aging research. Chad Slawson has received a $30,000 pilot grant for initial research into how mitochondria disrupt O-GlcNAc

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signaling in Alzheimer’s diseases. The title is the following: Alzheimer’s Mitochondria Defects Alter O-GlcNAc Signaling

Invited Seminars

1. Slawson, Chad. “The O-GlcNAc Post-Translational Modification: New Insights into Cellular Function”. KUMC Cancer Center, May 7, 2013.

2. Slawson, Chad. “The O-GlcNAc Post-Translational Modification: A Critical Regulator of Cellular Function”. University of Kansas Biophysics Seminar, Department of Physics and Astronomy, March 28, 2013.

3. Slawson, Chad. “O-GlcNAc Signaling Regulates Mitochondrial Function: Implications for Alzheimer’s Disease”. KUMC Translational Research Forum, March 22, 2013.

4. Slawson, Chad. “The O-GlcNAc Post-Translational Modification: A Critical Regulator of Cellular Function”. Kansas State University, Department of Diagnostic Medicine and Pathobiology, March 14, 2013.

5. Slawson, Chad. “O-GlcNAc Signaling Regulates Mitochondrial Function:

Implications for Alzheimer’s Disease.” KUMC Alzheimer’s Center Research Colloquium, Dec. 14, 2012.

6. Slawson, Chad. “Novel Roles for O-GlcNAc Signaling in the Regulation of Mitosis and Gene Transcription”. University of Lausanne, Switzerland, Center for Integrative Genomics, Aug. 31, 2012.

7. Slawson, Chad. “The O-GlcNAc Post-Translational Modification is a Key Regulator of Cellular Function”. University of Kansas School of Medicine, Department of Pharmacology, Jan. 31, 2012.

8. Slawson, Chad. “Controlling Cell Growth and Development with Sugar!” Northwest Missouri State University, Department of Chemistry, Nov. 17, 2011.

9. Slawson, Chad. “O-GlcNAc Signaling in Growth and Development”. University of Kansas, Protein-Structure Core, Nov. 2, 2011.

10. Slawson, Chad. “O-GlcNAcylation: A New Way to Regulate Mitosis”. Pittsburgh State University, Department of Chemistry, March 4, 2011.

11. Slawson, Chad and Hart, Gerald W. “Controlling M Phase Progression by O-GlcNAcylation”. University of Kansas, Department of Biochemistry and Molecular Biology, April 5, 2010.

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12. Slawson, Chad; Wang, Zihao; Udeshi, Namrata; Compton, Philip;

Shabanowitz, Jeffery; Hunt, Donald F.; and Hart, Gerald. “Extensive Crosstalk Between GlcNAcylation and Phosphorylation Regulates Cytokinesis”. FASEB Summer Research Conference, July 29, 2009.

13. Slawson, Chad and Hart, Gerald W. “The Sweet Side of M Phase: The Regulation of Mitosis by O-GlcNAc”. Texas A&M University, Department of Biochemistry and Biophysics, February 9, 2009.

14. Slawson, Chad and Hart, Gerald W. “Dynamic Regulation of the Cell Cycle by O-GlcNAc Post-translational Modifications”. University of Iowa, Department Anatomy and Cell Biology, November 24, 2008.

15. Slawson, Chad and Hart, Gerald W. “The Emerging Role of O-GlcNAc in

Mitotic Regulation”. 2008 Society for Glycobiology Annual Meeting, November 13, 2008.

16. Slawson, Chad and Hart, Gerald W. “O-GlcNAc Transferase and O-

GlcNAcase Form a Mitotic Complex with Aurora Kinase B at M Phase to Regulate the Post-translational Modifications of the Intermediate Filament Protein Vimentin”. National Institute of Health Glycoscience Day, Bethesda, MD. May 9, 2008.

17. Slawson, Chad and Hart, Gerald W. “Cell Cycle Regulation by O-GlcNAc

Post-translational Modifications”. Oklahoma Medical Research Foundation, Program in Cell Cycle and Cancer Biology, February 27, 2008.

18. Slawson, Chad and Hart, Gerald W. “The Evolving Role of O-GlcNAc in

Cell Cycle Control and mTOR Signaling.” Colorado State University, Department of Biochemistry And Molecular Biology, February 21, 2008.

19. Slawson, Chad and Hart, Gerald W. “Regulation of the Cell Cycle and

mTOR Signaling by O-GlcNAcylation.” University of Memphis, Department of Biology, February 7, 2008.

20. Slawson, Chad and Hart, Gerald W. “New Frontiers in Protein Regulation:

The Role of O-GlcNAc in Cell Cycle Control and mTOR Signaling.” University of Missouri, Department of Biochemistry, Jan. 29, 2008.

21. Slawson, Chad and Gerald Hart. “A New Look at Growth and Proliferation:

The Role of O-GlcNAc in Cell Cycle Control and mTOR Signaling.” Virginia Commonwealth University School of Medicine, Jan. 17, 2008.

22. Slawson, Chad and Hart, Gerald W. “The Emerging Role O-GlcNAc in Cell

Cycle Control and mTOR Signaling.” University of Akron, Department of Chemistry, Dec. 3, 2007.

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23. Slawson, Chad and Hart, Gerald W. “Vimentin is a Target of an O-

GlcNAc/O-Phosphate Signaling Complex at M phase.” 2007 American Society for Biochemistry and Molecular Biology Meeting, Washington D.C. April 30, 2007.

24. Slawson, Chad and Hart, Gerald W. “Dynamic Interplay Between O-

GlcNAc and O-Phosphate: Implications for Cancer, Diabetes, and Cardiovascular Disease.” Opening Lecture of the 17th Annual University of Alabama Birmingham Vascular Biology and Hypertension Symposium, Birmingham, AL. September 20, 2006.

25. Slawson, Chad; Pidala, Joseph; and Potter, Robert. “Decreases in N-

acetylglucosamine Modified Proteins Correlate with Increased N-acetyl-β-glucosaminidase Activity in Primary Breast Tumors.” 2000 Suncoast Biomolecular Science Conference, Tampa, FL. October 15, 2000.

26. Slawson, Chad and Potter, Robert. “Teaching the Undergraduate Science

Laboratory.” Graduate Teaching Workshop, Center for Teaching Enhancement, University of South Florida, Tampa, FL. August 2000.

27. Slawson, Chad and Potter, Robert. “Human Breast Tumors Show

Increased Cytoplasmic Protein Glycosylation,” 1998 Suncoast Biomolecular Science Conference, Tampa, FL. October 9, 1998.

28. Slawson, Chad and Potter, Robert. “N-acetylglucosamine, Novel

Cytoplasmic Sugar Modification at Potential Phosphorylation Sites: Proliferative Induced Changes in Modification.” Moffitt Breast Cancer Research Conference, Tampa, FL. July 23, 1996.

Poster Presentations

1. Antonio Artigues, Ee Phie Tan, Maria Villar, J. Lu, Eva Selfridge, Russell Swerdlow, and Chad Slawson. O-GlcNAc Signaling Regulates Mitochondrial Function: Implications for Alzheimer’s Disease. ASMBM Special Symposia, Post-Translational Modifications: Detection and Physiological Role, October 11-14, 2012

2. Ee Phie Tan, Anish Potnis, and Chad Slawson. The Regulation of Aurora Kinase B by O-GlcNAcylation. Experimental Biology Meeting, April 21, 2012.

3. Melody Chambers and Chad Slawson. Identification of OGT Interacting Proteins at M Phase. Experimental Biology Meeting, April 21, 2012. Won best poster in the DNA Replication, Recombination, Repair thematic session.

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4. Stephen Whelan, Ee Phie Tan, Catherine Costello, Mark McComb, and

Chad Slawson. Quantitative Proteomic Analysis of Proteins and Post-Translational Modifications During the Metaphase-Anaphase Transition after Altered O-GlcNAcylation. Experimental Biology Meeting, April 21, 2012

5. Xiaofei Gao, Rachel Olsen, Chad Slawson, Philip R. Hardwidge. A

bacterial virulence factor targets TRAF proteins to impair the host immune response. Harnessing Immunity to Prevent & Treat Disease Cold Spring Harbor Meeting 2011

6. Ramirez-Correa, Genraro; Slawson, Chad; Gong, Weidong: Hart, Gerald

W.; and Murphy, Anne M. Increased Cardiac O-GlcNAc Transferase and O-GlcNAcase Association to Actin, Tropomysin and MLC-1in Diabetes: A Mechanism for O-GlcNAc Mediated Myofilament Calcium Desensitization. Annual Biophysics Society Meeting, March 7, 2011.

7. Slawson, Chad; Wang, Zihao; Udeshi, Namrata; Compton, Philip; Shabanowitz, Jeffery; Hunt, Donald F.; and Hart, Gerald. “Extensive Crosstalk Between GlcNAcylation and Phosphorylation Regulates Cytokinesis”. FASEB Summer Research Conference, July 29, 2009.

8. Slawson, Chad; Wang, Zihao; Udeshi, Namrata; Compton, Philip; Shabanowitz, Jeffery; Hunt, Donald F.; and Hart, Gerald. “Identification and Quantification of Phosphorylated and O-GlcNAcylated Proteins Associated with Mitotic Spindles and Midbodies during Cytokinesis”. 2009 American Society for Mass Spectrometry Annual Meeting, June 2, 2009.

9. Slawson, Chad and Hart, Gerald. “The Emerging Role of O-GlcNAc in Mitotic

Regulation”. 2008 Society for Glycobiology Annual Meeting, November 13, 2008.

10. Slawson, Chad and Gerald Hart. “O-GlcNAc Transferase and O-GlcNAcase Form a

Mitotic Complex with Aurora Kinase B at M Phase to Regulate the Post-translational Modifications of the Intermediate Filament Protein Vimentin”. National Institute of Health Glycoscience Day, Bethesda, MD. May 9, 2008.

11. Slawson, Chad and Hart, Gerald W. “Vimentin is a Target of an O-GlcNAc/O-Phosphate

Signaling Complex at M phase.” 2007 American Society for Biochemistry and Molecular Biology Meeting, Washington D.C. April 30, 2007.

12. Slawson, Chad and Hart, Gerald W. “O-GlcNAc Transferase is a Critical Regulator of Cytokinesis: 2006 American Society for Biochemistry and Molecular Biology Meeting, San Francisco, CA. April 1, 2006.

13. Slawson, Chad; Zachara, Natasha; Cheung, Win D.; Vosseller, Keith.; Lane, Malcolm D.; and Hart, Gerald W. “Dynamic Regulation of O-GlcNAc During the Mammalian Cell Cyle” 2004 American Society for Biochemistry and Molecular Biology Meeting, Boston, MA. June 17, 2004.

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14. Slawson, Chad; Whelan, Stephen; and Potter, Robert. “Relationship Between Nuclear and Cytoplasmic Protein O-Glycosylation and Mitogen Activated Protein Kinase Activity in Breast Cancer Cell Lines.” 2001 American Society for Biochemistry and Molecular Biology Meeting, Orlando, FL. April 3, 2001.

15. Slawson, Chad; Pidala, Joseph; and Potter, Robert. “Increased N-Acetyl-β-glucosaminidase Activity in Primary Breast Tumors Corresponds to a Decrease in Serine/Threonine N-Acetylglucosamine Modified Proteins.” 2000 American Society for Cell Biology Meeting, San Francisco, CA. December 9, 2000.

16. Slawson, Chad; Pidala, Joseph; and Potter, Robert. “Increased N-

Acetylglucosaminidase Activity in Breast Tumor Tissue.” 2000 Annual American Chemical Society Meeting Florida Chapter, Orlando, FL. May 13, 2000.

17. Slawson, Chad; Beakey, Paul; and Potter, Robert. “Novel Cytoplasmic Sugar

Modification at Potential Protein Phosphorylation Sites: Proliferative Induced Changes in N-acetylglucosamine Modification.” 1996 Joint Annual American Chemical Society Meeting, Orlando, FL. August 26, 1996.

18. Slawson, Chad; Beakey, Paul; and Potter, Robert. “Proliferative Induced Changes in the

Level of N-acetylglucosamine Protein Modification.” 1996 Suncoast Biomolecular Science Conference, Tampa, FL. November 4, 1996.

Student Presentations

1. Zhen Zhang, Ee Phie Tan, Anish Potnis, Nathan Bushue, Flavia Costa, Kenneth

Peterson, and Chad Slawson. “O-GlcNAcylation Regulates γ-Globin Transcription”. ASMBM Special Symposia, Post-Translational Modifications: Detection and Physiological Role, October 11-14, 2012

Teaching Experience

• Lecturer, University of Kansas School of Medicine, Biochemistry, BCHM 922, October 15-24, 2012

• Co-director, University of Kansas School of Medicine, Biochemistry 862, August 2012 - present

• Lecturer, University of Kansas School of Medicine, Cell Communication: Receptor Tyrosine Kinases, March 2012 - present.

• Lecturer, University of Kansas School of Medicine, Foundations of Medicine, Apoptosis Lecture, 2011-present; Cell Cycle Lecture, 2011 - present; and Mitosis & Meiosis Lecture, 2011- present

• Small Group Discussion Leader, University of Kansas School of Medicine, Foundations of Medicine, Sickle Cell Disease, 2011 - present; Mitochondrial DNA & Disease, 2011 – present, and Huntington’s Disease, 2012 - present

• Lecturer, University of Kansas School of Medicine, Pre-matriculation Course, Cell Cycle Lecture, 2011 - present

• Instructor, University of South Florida, Biochemistry Laboratory, 1996 – 2002

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• Instructor, University of South Florida, General Chemistry Laboratory, 1994-1996

Mentoring

Current Students

• Ee Phie Tan, 1st year Interdisciplinary Graduate Program Biomedical Science

Student, University of Kansas School of Medicine, Project: O-GlcNAcylation Regulates Mitoic Spindle Function, 2012-present

• Zhen Zhang, 2nd year Interdisciplinary Graduate Program Biomedical Science Graduate Student, University of Kansas School of Medicine, Project: Transcriptional Regulation by O-GlcNAc, 2012-present

Past Students

• Chris Lanza, Undergraduate Researcher from Santa Cruz University, University of Kansas

School of Medicine, Project: Spindle Function is Regulated by O-GlcNAcase, 2012 • Melody Chambers, Undergraduate Researcher from Rockhurst University, University of

Kansas School of Medicine, Project: Determining the OGT Mitotic Interactome, 2011 • Anish Potnis, Undergraduate Researcher from Georgia Institute of Technology, University

of Kansas School of Medicine, Project: The Role of O-GlcNAcylation in Regulating the Aurora Kinase B Phosphorylation Circuit, 2011 and 2012

• Olof Lagerlof, Visiting Medical Student from the University of Gothenburg Sweden, Johns Hopkins Medical Institute, Project: The Effects on Neuronal Cell Differentiation by O-GlcNAc, 2007

• Luna Alamar, Biochemistry, Cellular, and Molecular Biology Ph.D. Program Laboratory Rotation, Johns Hopkins Medical Institute, Project: O-GlcNAcylation of Protein RB, 2007

• John Bullen, Biochemistry, Cellular, and Molecular Biology Ph.D. Program Laboratory Rotation, Johns Hopkins Medical Institute, Project: The Role of O-GlcNAc on Glut-4 Vesicle Transport, 2006

• Jeremy Rotty, Biochemistry, Cellular, and Molecular Biology Ph.D. Program Laboratory Rotation, Johns Hopkins Medical Institute, Project: TATA-Binding Protein is Modified by O-GlcNAc, 2005

• Michael Housley, Biochemistry, Cellular, and Molecular Biology Ph.D. Program Laboratory Rotation, Johns Hopkins Medical Institute, Project: O-GlcNAcylation in Yeast, 2003

• Susan Shafii, Honors Chemistry, University of South Florida, Project: O-GlcNAcase Activity in Progesterone Stimulated Xenopus laevis Oocytes, 2002

• James Amburgey, Honors Chemistry, University of South Florida, Project: Characterization of O-GlcNAcylation During Xenopus laevis Oocyte Maturation, 2001

• Joseph Padila, Honors Chemistry, University of South Florida, Project: Increased N-Acetylglucosaminidase Activity in Human Breast Tumors, 2000

• Pedro Troya, Honors Chemistry, University of South Florida, Project: Characterization of O-GlcNAc Modified Proteins in Human Breast Tumor Tissue, 2000

• Timothy Ragan, Honors Chemistry, University of South Florida, Project: Novel Partial Purification of Soluble Monoglycosylated Proteins, 1999

• Javier Lopez, High School Math and Science Camp Individual Honors, University of South Florida, Project: Measurement of O-GlcNAc in Xenopus laevis Oocyte Stages, 1998

• Fay Yao, High School Math and Science Camp Individual Honors, University of South Florida, Project: Measurement of O-GlcNAc in Xenopus laevis Oocyte Stages, 1998

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• Chakree Tanjaroon, Honors Chemistry, University of South Florida, Project: Detection of

Protein Glycosylation in the Cytoplasm and Nucleoplasm of Xenopus laevis Oocytes, 1998 • William Munroe, Honors Chemistry, University of South Florida, Project: Investigating the

Role of Nucleoside Diphosphate Kinase in Xenopus laevis Oocyte Maturation, 1998 • Parul Khator, High School Math and Science Camp Individual Honors, University of South

Florida, Project: Measurement of O-GlcNAc in Stage 6 Xenopus laevis Oocytes, 1997

Student Committees

• Tanja Bhuiyan, Dissertation Committee, Dr. Winship Herr Laboratory, Center for

Integrative Genomics, University of Lausanne, Switzerland, Spring 2012 - present

• Allen Chazelle, Dissertation Committee, Dr. Kenneth Peterson Laboratory, University of Kansas School of Medicine, Spring 2012 – present

• Eva Selfridge, Dissertation Committee, Dr. Russell Swerdlow Laboratory, University of Kansas School of Medicine, Fall 2011- present

• Jason Barnett, Orals Committee, Dr. Aron Fenton Laboratory, University of Kansas School of Medicine, Fall 2011

Service

University of Kansas

• Academic and Professionalism Committee: Students Promotions and Special

Programs Sub-committee, University of Kansas School of Medicine, Fall 2012 – present

• Medical Student Interviews, University of Kansas School of Medicine, Fall 2012 - present

• Heartland Undergraduate Biochemistry Forum Committee, University of Kansas School of Medicine, Spring 2011 – present

Scientific Service

• Session Chair, PTM Identification Methodology and Tools: ASMBM Special

Symposia, Post-Translational Modifications: Detection and Physiological Role, October 11-14, 2012

• Ad Hoc reviewer for the Journal of Proteomics Research 2012 - present • Ad Hoc reviewer for the Journal of Biological Chemistry 2011 - present

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Date: 11/15/2013

UNIVERSITY OF KANSAS SCHOOL OF MEDICINE Faculty Curriculum Vitae

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I. PERSONAL DATA

First Name: Last name:

Jinxi Wang

Current Academic Rank: Department(s):

Office Address: Phone:

Professor (tenured) Orthopedic Surgery (Primary), Biochemistry and Molecular Biology (Joint appointment) 3901 Rainbow Boulevard, Smith East 4010A, Mail-Stop 3017, Kansas City, KS 66160 913-588-0870

Fax: Email:

913-495-7773 jwang@kumc.edu

PROFESSIONAL DEVELOPMENT

Undergraduate and Graduate Education

Years Degree Institution

1987 M.D. (Medicine) Suzhou Medical College, Suzhou, China

1991 Ph.D. (Cell Biology) Suzhou Medical College and Loma Linda University, Loma Linda, CA

Postgraduate Education

Years (Inclusive) Degree Institution

1987-1992 Orthopaedic Residency Suzhou Medical College Hospital

1992-1995 Advanced Coursework in Bone Biology Harvard Medical School, Boston, MA

1992-1996 Research Fellow in Molecular and Cellular Biology of Skeletal Tissues

Harvard Medical School and Children’s Hospital, Boston, MA

Academic and Professional Appointments and Activities (List in chronological order. Please explain any discontinuity in professional experience)

Month and Year Position Institution

July/1996 - Dec/2004 Instructor in Orthopaedics Harvard Medical School, Boston, MA

July/1996 - Dec/2004 Staff Scientist, Department of Orthopaedic Surgery

Children’s Hospital, Boston, MA

Dec/2004 - June/2012 Associate Professor of Orthopedic Surgery (primary appointment)

University of Kansas, School of Medicine, Kansas City, KS

Jan/2006 – June/2012 Associate Professor of Biochemistry & Molecular Biology (secondary appointment)

University of Kansas, School of Medicine, Kansas City, KS

Dec/2004 - present Director, The Harrington Laboratory for Molecular Orthopedics

University of Kansas, School of Medicine, Kansas City, KS

Nov/2011 - present Mary A. & Paul R. Harrington Distinguished Professor in Molecular Orthopedics

University of Kansas, School of Medicine, Kansas City, KS

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July/2012 - present Professor of Orthopedic Surgery (primary) and

Biochemistry & Molecular Biology (secondary) University of Kansas, School of Medicine, Kansas City, KS

Professional Registration/Licensure N/A (I am not practicing medicine)

Year Number State

N/A

Professional Certification(s) N/A

Date Board

N/A

Professional Societies and Affiliations

Date Organization (including offices held)

1995- present Member, American Society for Bone and Mineral Research

1996- present Member, International Bone and Mineral Society

2002- present Member, International Association for Biological & Medical Research (IABMR)

2002- 2012 Board Director (for development and membership) of IABMR

2003- present Active Member, Orthopedic Research Society (U.S.A.)

2007- 2008 President-Elect, Sigma Xi Scientific Research Society KUMC Chapter

2008- 2009 President, Sigma Xi Scientific Research Society KUMC Chapter

2009-2010 Past-Present, Sigma Xi Scientific Research Society KUMC Chapter

2012-present Member, Osteoarthritis Research Society International

Honors and Awards (honorary societies, research awards, teaching and other awards)

Year Award

1991 Young Investigator Research Award, granted by the Department of Public Health, Jiangsu Province, R. R. China

1994 Research Award for Outstanding Research Achievement in Pathogenesis of Osteoarthritis Following Osteonecrosis of the Femoral Head, granted by the Ministry of Health, P. R. China

1998 Young Investigator Award, granted by the Executive Board of the 6th

International Conference on Chemistry and Biology of Mineralized Tissues, Vittel, France

1998 National Institutes of Health (NIH, U.S.A.) Research Service Award 2011 The Mary A. & Paul R. Harrington, MD, Distinguished Professorship

in Molecular Orthopedics

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II. TEACHING ACTIVITIES

(Teaching evaluations and other evidence of quality teaching must be attached; this represents the teacher’s portfolio and should be accurately summarize ALL of your teaching activities.)

Brief statement of areas of teaching interest:

I thoroughly enjoy my opportunities to teach and participate in the training of learners in musculoskeletal biology and diseases. I have been teaching medical, dental and graduate students, orthopedic residents, and postdoctoral research fellows since 1996 in the following areas: (a) bone and cartilage biology, (b) bone and cartilage regeneration, (c) mechanisms for mineralization of bone and teeth, and (d) specific skeletal diseases such as osteonecrosis and osteoarthritis.

During my own training and school courses, I found that teachers who focused on problem solving skills and utilized multiple sources, such as textbooks, additional materials, and personal experiences, stimulated my intellectual curiosity and ability to function as a lifetime learner. In my lectures and laboratory teaching, I use heuristic educational styles to encourage my students, postdoctoral fellows, and residents not only to memorize the basic knowledge from textbooks and lectures, but also to be creative, innovative problem solvers. I try to stimulate higher learning beyond textbooks. I also use various methods and vary my expectations for different learners at bachelor, graduate, and postgraduate levels to meet their specific needs.

At KU School of Medicine, one of my major teaching activities has been focused on teaching orthopedic residents in the area of orthopedic basic sciences with a focus of skeletal biology. My M.D., Ph.D., and orthopedic residency training background enables me to effectively integrate orthopedic basic sciences with clinical application through both formal lectures and group discussion (e.g. Journal Club which is focused on publications from the Journal of Bone & Joint Surgery and other orthopedic journals). As an instructor for the Foundations of Medicine and Musculoskeletal Modules at KU School of Medicine, I have presented Bone & Cartilage Histopathology to 1st year medical students and Bone

& Joint Histopathology to 2nd year medical students, respectively.

In addition, I teach histology, cell biology, biochemistry, and molecular biology of skeletal tissues, as well as manuscript and grant writing, to pre-medical students, medical and graduate students, postdoctoral fellows, and orthopedic residents through daily research activity in the orthopedic research laboratory.

Instruction:

Didactic (e.g.: lectures and formal presentations)

Academic Year

Course

Title Instruction Student

Type Hours No Type

2001- 2004

Oral Biology 604B

New trends in mineralized tissue regeneration

Lecture 12/year 25-30 Dental Students, Harvard Dental School

2005- Present

Orthopedic Basic Sciences

Bone and cartilage development

Lecture 3-6/year 15-20 KU Orthopedic Residents

2005- Present

Orthopedic Basic Sciences

Basic biology of skeletal cells

Same 3-6/year 15-20 KU Orthopedic Residents

2005- Present

Orthopedic Basic Sciences

Bone modeling and remodeling

Same 3-6/year 15-20 KU Orthopedic Residents

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2005- Present

Orthopedic Basic Sciences

Function of the growth plate

Same 3-6/year 15-20 KU Orthopedic Residents

2005- Present

Orthopedic Basic Sciences

Cartilage composition & metabolism

Same 3-6/year 15-20 KU Orthopedic Residents

2005- Present

Orthopedic Basic Sciences

Articular cartilage repair

Same 3-6/year 15-20 KU Orthopedic Residents

2005- Present

Orthopedic Basic Sciences

Osteoarthritis (etiology & biochemistry)

Same 3-6/year 15-20 KU Orthopedic Residents

2005- Present

Orthopedic Basic Sciences

Fracture healing Same 3-6/year 15-20 KU Orthopedic Residents

2005- Present

Orthopedic Basic Sciences

Bone defect repair Same 3-6/year 15-20 KU Orthopedic Residents

2005- Present

Orthopedic Basic Sciences

Basics of molecular biology

Same 3-6/year 15-20 KU Orthopedic Residents

2005- Present

Orthopedic Basic Sciences

Bone biology lab techniques

Same 3-6/year 15-20 KU Orthopedic Residents

2011- Foundations of Medicine Module

Bone & Cartilage Histopathology

Presentation 3/year 25 1st year medical students, KU School of Medicine

2011- Musculoskeletal Module

Bone & Joint Histopathology

Presentation 3/year 25 2nd year medical students, KU School of Medicine

2012- Advanced Rehabilitation Sciences

Structure of synovial joints and osteoarthritis

Presentation 3/year 3 Graduate students, KU School of Health Professions

Nondidactic (e.g.: workshops, labs, and discussion groups)

Academic Year

Course

Title Instruction Student

Type Hours

No Type

1996-2004 Bone Histology Microstructure of mouse joints

Lab discussion groups

60/year 1 - 10 Harvard graduate and med students

1999-2004 Bone Histology Microstructure of cortical bone

Lab discussion groups

30/year 1 - 6 Harvard graduate and med students

2005-2006 Bone Biology Bone mineralization

Research lab training

80/year 1 Andy Anderson, KU medical student

2006-2007 Bone Biology Bone regeneration

Research lab training

80/year 1 John Yost, KU Pre- medical student

2008

(May-July)

Bone Biology Bone regeneration

Research lab training

10/week 1 John Yost, KU medical student

2009

(May-July)

Cartilage Biology

Osteoarthritis Research lab training

10/week 1 M. Kareem Shaath, KU Pre-medical student

2009-2010 Cartilage Biology

Osteoarthritis Research lab training

10/week 1 Brian Gardner, KU premedical student

2010 (May-July)

Cartilage Biology

Osteoarthritis Research lab training

10/week 1 M. Kareem Shaath, KU medical student

2010-2011 Cartilage Biology

Human osteoarthritis

Research lab training

10/week 1 James Bernard, KU premedical student

2011 (May-July)

Cartilage Biology

chondrocyte differentiation

Research lab training

10/week 1 Clayton Theleman KU medical student

2011 (May-July)

Bone Biology BSP in osteogenesis

Research lab training

10/week 1 John Garlish, KU medical student

2007- Present

Journal Club Clinical & basic orthopedics

Discussion groups 8/year 15-20 KU orthopedic residents

Clinical: N/A

Year

Hours Student Length of Service

No Type N/A

Master’s Theses and PhD Dissertations directed

Year

Student Name

Thesis Title

Degree (Completed/In process)

1996-1999

John C. Huang

Protein kinases and physpho- proteins in

developing enamel

Doctor of Medical Sciences Harvard School of Dental

Medicine - Completed

1998-2000

Susan So Osteopontin and bone sialoprotein as a

function of turkey tendon mineralization

Master in Medical Sciences Harvard School of Dental

Medicine - Completed

2000-2002

Margo T. Kusienski Immunohistological and biochemical studies on dentin phosphoproteins

Master in Medical Sciences Harvard School of Dental

Medicine - Completed

2001-2003

Joseph Hung The efficacy of alloplastic bone implants

on the healing of rat calvarial defects

Master in Medical Sciences Harvard School of Dental

Medicine - Completed

2001-2003

Robert J. Bruzzichesi Characterization of extracellular matrix

phosphoproteins of cementum

Master in Medical Sciences Harvard School of Dental

Medicine - Completed

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2013- Nathan Wilson SPECC1L modulation of adherens

junctions for neural crest cell function during craniofacial morphogenesis

PhD in Cell Biology University of Kansas

School of Medicine – In process

2013-

Sushma

Jadalannagari

Decellularized Wharton’s Jelly Matrix to accelerate bone Healing in a murine

calvarial bone defect model

PhD in Biomaterials and Tissue Engineering

Supervision of Postdoctoral Fellows/Residents

Year Fellow/Resident Name Area of Study

2001-2002 Jun Yan, M.D. Osteonecrosis and osteoarthritis

2002-2005 Jochen Hofstaetter, M.D. Osteonecrosis and osteoarthritis

2002-2004 Lan Xu, Ph.D. In vivo function of bone sialoprotein

2006-2007 Rama Garimella, Ph.D. Cellular and molecular biology of skeletal tissues

2006-2010 Marianna Rodova, Ph.D. Cellular and molecular biology of skeletal tissues

2008-2010

Brent Woodbury, M.D. (Orthopedic Resident)

Molecular mechanisms of osteoarthritis

2009-2010

J. Paul Schroeppel, M.D. (Orthopedic Resident)

Articular cartilage biology and its repair

2010-2013

William Kramer, M.D. (Orthopedic Resident)

Mechanisms of post-traumatic osteoarthritis

2011-

Kenneth Caldwell, M.D. (Orthopedic Resident)

Articular cartilage repair

2011- Mingcai Zhang, Ph.D. Bone sialoprotein and osteogenesis

2012- Yi Feng, Ph.D. NFAT1 deficiency and osteoarthritis

2013-

Paul Cowan, M.D. (Orthopedic Resident)

Biomaterials in cranial bone repair

Advising (Thesis or dissertation defense committees: student academic group/individual)

Date Student or group name Type of Student/group

04/23/1999

John C. Huang’s dissertation committee

Ph.D. Student, Harvard School of Dental Medicine

04/05/2000 Susan So’s thesis committee Master Student, Harvard School of Dental Medicine

05/02/2002 Dasha Ellezian’s thesis committee Master Student, Harvard School of Dental Medicine

TBD

Nathan Wilson’s dissertation committee

PhD Student, University of Kansas School of Medicine

TBD

Sushma Jadalannagari’s dissertation committee

PhD Student, University of Kansas School of Engineering

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Other teaching activities

Date Title Place Teaching Function

June-July/2010

Bone biochemistry and molecular biology

Harrington Laboratory for Molecular Orthopedics, KUMC

Supervised Armand Heyns, post-baccalaureate intern

Development of Educational Materials

(Course materials e.g. syllabi, educational software packages, web sites, films, educational tapes and evaluation tools)

In addition to the development of PowerPoint and handout teaching materials for orthopedic resident lectures, I have developed a number of laboratory protocols to help students and fellows/residents understand basic principles and practical experimental procedures. Most of these procedures are developed for my specific

projects and are technically difficult. My protocols have made these complex procedures easy to understand and follow, which have significantly shortened students/trainees’ learning time and improved their research performance. These protocols are listed below.

Year Title Description Intended Audience

2006

Purification of bone sialoprotein from bovine bones Pre-med, med/grad students,

research fellow, residents

2007 Isolation of RNA from rodent articular cartilage tissue Same

2008 RNA/DNA isolation from human cartilage tissue Same

2009 RNA isolation from cultured articular chondrocytes Same

2010 Purification of bone sialoprotein from rat bones Same

Educational Leadership

(Responsibility for courses and other leadership activities including mentoring of junior faculty.

Please list faculty members mentored)

I joined the faculty of Orthopedic Surgery in 2004. My major mission was to establish and develop a new bone biology laboratory for both research and education. As a lab director, I have developed and led a new biology laboratory named “The Harrington Laboratory for Molecular Orthopedics.” This laboratory is well organized and equipped with instruments not only for scientific research but also for education, such as micro-dissection and presentation of bone and cartilage cells, bone microstructure, function of various skeletal cells, and histopathology of skeletal tissues. This educational lab setting has provided a learning environment to greatly help medical students and orthopedic residents better understand the morphology and cellular/molecular biology of the skeletal system, which is an important part of their required examinations. For example, Step 1 of USMLE (for medical students) and Orthopedic In-Training Examination (OITE, for orthopedic residents) contain basic sciences of the skeletal system.

At the national level, I have been serving as a member of the New Investigator Mentoring Committee of the Orthopedic Research Society (ORS, USA) since February of 2012 to assist junior faculty members and new scientists with grant writing and career development. I have pre-reviewed grant applications from junior faculty members at other universities and provided my comments and suggestions to help

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them improve their proposals prior to official submission. In addition, I have given lectures at the Grant Writing Workshops sponsored by ORS.

III. SERVICE ACTIVITIES

See guidelines and instructions to applicants for definitions and suggested documentation of professional and academic service.

Professional Service: N/A

The diverse area of professional service includes patient care. Applicants should select measures that most clearly and concisely document their accomplishments and the value of these activities to the Medical School and University. Measures of both quantity and quality of activities are required and if necessary, applicants should provide brief descriptions to assist reviewers. Measures of patient care activities include numbers of patients, time allocation in clinical activity, procedures completed, Relative Value Units (RVUs), and value to the School of the clinical service. If the primary quality evaluation is the subjective assessment of peers, this should be available in letters from departmental colleagues, chair, or referees.

The significance of professional service in the forms of task forces, committees and similar groups should be explained and the specific role of the applicant clarified.

Professional consulting services must have academic credibility and clear service intent and not be performed primarily for personal profit.

Academic Service:

In academic service the contribution of the candidate to the academic community should be clearly documented. Names and dates of committees, task forces, or working groups should be provided. A concise description of the significance of the group and explanation of the role of the applicant should be provided.

Activities related to Academic Societies should be documented in this section.

Intramural service.

(A) I have served on the Research Committee in the Orthopedic Surgery Department at KUMC to assist the Committee with organization and supervision of student and resident research since 2005.

(B) I served on the KUMC Biomedical Research Training Grant Applications Review Committee and reviewed grant applications from postdoctoral fellows and pre-doctoral students in 2007, 2008, and 2010.

(c) I have served as a regular voting member of the KUMC Institutional Animal Care and Use Committee (IACUC), representing the Surgery Departments, since February 1, 2011 and will continue this service until January 31, 2014. As an IACUC Committee member, I am responsible for reviewing animal protocols, addenda, and standard of animal procedures. Also, I am required to attend IACUS meetings and inspect animal facilities.

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Extramural service.

(A) Committee member/officer of professional organizations

2002-2012 Board Director for development and membership of International Association for Biological and Medical Research (IABMR)

2007-2008 President-Elect of Sigma Xi Research Society KUMC Chapter (Sigma Xi is an

international scientific research society with more than 60,000 active members)

2008 - 2009 President of Sigma Xi Research Society KUMC Chapter

2012- Member, the New Investigator Mentoring Committee, Orthopedic Research Society (ORS, USA)

(B) Conference organizing committee/session chair

2007 - 2009 Member of Organizing Committee, Sigma Xi KUMC Chapter monthly seminar

May/2008 Program Chair, Sigma Xi KUMC Chapter Annual Symposium

Aug/2012 Organizing Committee Member and Scientific Advisor, 2012 International Conference on Orthopedics and Rheumatology, August 13-15, 2012, Chicago, IL

Aug/2012 Session Chair: Regenerative and Molecular Orthopedics Session, 2012 International Conference on Orthopedics and Rheumatology, August 13-15, 2012, Chicago, IL

Jan/2013 Session Moderator: New Investigator Recognition Award Session 2 – Bone.

ORS 2013 Annual Meeting, San Antonio, TX, USA, January 26-29, 2013.

Oct/2013 Session Moderator: Oral Poster Presentations - Late-Breaking

ASBMR 2013 Annual Meeting, Baltimore, MD, USA, October 4-7, 2013.

(C) Abstract reviewer 2008 Reviewed 120 Abstracts in the area of “Disorders of Bone and Mineral Metabolism” for

the ASBMR (the American Society for Bone and Mineral Research) 30th Annual Meeting, Montreal, Canada, September 12-16, 2008.

2009 Reviewed 94 abstracts in the same area mentioned above for the ASBMR 31st Annual Meeting, Denver, CO, USA, September 11-15, 2009.

2011 Reviewed 51 abstracts in the areas of cartilage, synovium, meniscus, and osteoarthritis for the ORS 2012 Annual Meeting.

(D) Manuscript reviewer for the following

journals/books: 1997 - Journal of Cellular

Engineering

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2006 - Biomaterials, Biochemical Journal

2008 - A book chapter entitled “DNA Methylation and Osteoarthritis” in the Book Epigenetics of Aging

2009 - Acta Biomaterialia, Journal of Orthopaedic Research

2010 - Journal of Bone and Mineral Research, Calcified Tissue International

2012 - Arthritis & Rheumatism, Molecular Endocrinology, Journal of Orthopaedic Surgery and Research

2013 - Arthritis Research and Therapy

(E) Editorial Board

2011 - International Journal of Clinical and Experimental Medicine (Editor for bone

research) 2011 - Scientific World Journal (Editor for biomaterials and skeletal tissue

repair)

2011 - Frontiers in Craniofacial Biology

2012 - Journal of Bone and Mineral Research (JBMR)

(F) Grant Reviewer

2011- National Institutes of Health (NIH): Ad Hoc Member of NIH Skeletal Biology Structure and Regeneration (SBSR) Study Section.

2012- Israel National Science Foundation: reviewer for grant applications in skeletal biology and

diseases. 2012- Netherlands Organization for Health Research and Development, Independent

Grant Reviewer 2012- NIH: Ad Hoc Member of Special Emphasis Panel, Skeletal Pathology and

Orthopedics

2012- US Department of Defense (DoD), Peer Reviewed Medical Research Program (PRMRP), Member of the Peer Review Panel for Investigator Initiated Research Award (IIRA) in the topic area of post-traumatic osteoarthritis.

2013- NIH: Ad Hoc Member of NIH Skeletal Biology Development and Disease (SBDD) Study

Section. 2013- NIH: Ad Hoc Reviewer for the NIH Director’s Early Independence Awards (DP5

grants).

IV. RESEARCH and SCHOLARLY ACTIVITIES:

Brief statement of areas of research and scholarly interest, including current projects:

1. Bone sialoprotein and bone regeneration. Bone sialoprotein (BSP) is one of the major non-collagenous extracellular matrix (ECM) proteins in bone and tooth. BSP is highly expressed in developing and postnatal regenerating bone, but its precise function is largely unclear. My research group was the first to report that implantation of BSP with reconstituted type-I collagen stimulated osteoblast differentiation and bone formation during the repair of cranial bone defects in rodents. In contrast, no bone formation was observed when BSP-collagen was implanted into the thoracic subcutaneous soft tissue. The osteogenic bioactivity of BSP in stimulating bone regeneration is an unprecedented result; the mechanisms of tissue-specific BSP action remain unclear. The major goal of our studies is to explore the molecular and cellular

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mechanisms of tissue- specific BSP action in osteogenesis and its application for enhancing bone regeneration. Successful completion of this project will significantly advance our understanding of the mechanisms of BSP-specific biological activities, which will possibly lead to improved methods for the treatment of bone defects in humans. Moreover, understanding the cell type- and bone environment-dependent effects of BSP on osteogenesis may provide new insights into the long-standing question of why metastasis of BSP-expressing tumors is preferential to bone. This research project is currently supported by an NIH/NIDCR grant (R01 DE018713, PI: Jinxi Wang).

2. Pathogenetic mechanisms and therapeutics for osteoarthritis. Osteoarthritis (OA) is the most common form of joint disease. No proven pharmacologic therapy is currently available to prevent the initiation or reverse the progression of OA, largely because the root causes of OA remain unclear. My research interest in OA was initiated during the early studies for my Ph.D. thesis on the pathogenesis of secondary OA following osteonecrosis of the femoral head in humans. Recently, my research group discovered that mice lacking transcription factor NFAT1 exhibit normal skeletal development but display most of the characteristics of OA seen in humans. The objective of this project is to explore the molecular and cellular mechanisms underlying the pathogenesis of NFAT1 deficiency-associated OA in mice and possibly in humans. Successful completion of this project may provide a better understanding of the pathogenesis of OA and identify target cells of NFAT1 in the joint, thereby establishing a rationale for development of preventive and therapeutic strategies using NFAT1 as a molecular target for NFAT1 deficiency-induced OA. This project is currently supported by an NIH R01 grant (R01 AR059088, PI: Jinxi Wang).

3. Post-traumatic osteoarthritis. The objective of this project is to examine whether NFAT1 expression level in joint tissues affects the time of onset and severity of post-traumatic knee OA after surgical transection of the medial meniscotibial ligament in mice. This project is currently supported by a DoD grant (PI: Jinxi Wang).

4. Regulatory mechanisms of chondrocyte differentiation and articular cartilage regeneration. Currently, efforts to repair damaged articular cartilage face major obstacles due to limited intrinsic repair capacity of the tissue. Animal and human studies have demonstrated that a full thickness defect of articular cartilage penetrated through the subchondral bone to the bone marrow spaces can be repaired morphologically through the proliferation and differentiation of bone marrow mesenchymal stem cells into cartilage cells which synthesize a cartilage matrix or by implanting chondrocyte-seeded biomaterials with or without growth factors using tissue engineering technology. However, the repaired articular cartilage tissue degenerates with reduced expression levels of cartilage markers after several months, and the joints with articular cartilage lesions eventually develop osteoarthritis. The objective of this project is to investigate the regulatory mechanisms of chondrocyte differentiation and articular cartilage regeneration in mice and humans, thereby developing more effective therapeutic strategies for the healing of articular cartilage defects.

Through these projects, I have developed research expertise in the areas of bone cell differentiation and bone regeneration, chondrocyte function and articular cartilage regeneration, and pathogenesis of osteoarthritis.

1 Grants and contracts

(Information must include whether the nominee (name bolded) is the principal investigator or a co-

investigator, names of all investigators, title of grant, funding source, dollar amount in direct costs, and

years during which grant applies. Co- investigators must specify role). Provide the cover sheet, abstract

and Notice of Award in PDF for all grants or contracts awarded in last five years (submit online).

Previous Grants and contracts awarded: (List in chronological order – oldest first, newest last)

302

Principal

Investigator

Investigators

Title of Grant Funding Source

Direct Costs

Years

Status

Yaotang Wu

Jinxi Wang (Co-PI)

(in vivo tissue analyses)

Evaluation of solid state MRI of bone mineral and matrix

Orthopaedic Research & Education Foundation

(OREF)

$150,000

1997-2000

Completed

Jinxi Wang

Melvin Glimcher (Sponsor)

In vivo molecular and cellular responses to

osteogenic factors

NIH Research Fellowship

$116,000 1998-2001

Completed

Melvin Glimcher

Jinxi Wang

Jinxi Wang (Co-PI, animal

surgery and histopathology)

Use of alendronate to prevent osteoarthritis following osteonecro- sis of the femoral head

Arthritis Foundation

$150,000 2000-2003

Completed

Jinxi Wang

Osteogenic potential of bone sialoprotein

OREF $100,000

2001-2004

Completed

Melvin Glimcher

Jinxi Wang (Co-I, in vivo

mineralization) Yaotang Wu

(MRI analysis)

Nature of bone mineral: inception, maturation, aging

NIH/NIA R01 AG014701

$2,986,000

1997-2008

Completed

Jinxi Wang

Mechanism of Site- Specific Bone Differentiation Pathways

NIH/NIAMS

R03AR052088

$150,000 2006-2009

Completed

Current Grants and contracts awarded: (List in chronological order – oldest first, newest last)

Principal Investigator

Investigators

Title of Grant

Funding Source

Direct Costs

Years

Status

Jinxi Wang

Paul Trainor (signaling study)

Gerry Carlson (protein chem)

Bone Sialoprotein in Osteogenesis and Bone Regeneration

NIH/NIDCR

R01 DE018713

$1,032,000

2010-2015

Active

Jinxi Wang

H.C. Anderson (bone pathol)

Xiaobo Zhong

Transcription Factor NFAT1 Deficiency and Osteoarthritis

NIH/NIAMS R01

AR059088

$1,125,000

2011-2016

Active

(epigenetics) Cory Berkland (KU Lawrence)

Jinxi Wang

(PI on Subcontract )

Jinxi Wang (implantation

of biomaterials and bone healing

analyses)

Integrative Colloidal Gels for Cranial Defect Repair

NIH/NIDCR

R01 DE022472

$1, 035,000

2012-2016

Active

303

Jinxi Wang

Ken Brandt and A. Ahad

Haleem, (clinical

consultation)

Deficiency of Transcription Factor

NFAT1 and Posttraumatic Osteoarthritis

Department of Defense

(DoD)

W81XWH- 1210304

$750,000

2012-2015

Active

Grants and contracts submitted:

Principal Investigator

Investigators

Title of Grant

Funding Source

Direct Costs

Years

Status

Xinmai Yang

Jinxi Wang

(subcontract PI)

Mitigate Osteoarthritis by

Shear Wave

DoD

$500,000

2014-2017

Pending

2 Scholarly Publications

Full length, peer-reviewed articles in scientific journals: (Provide names of all authors, year, title, journal, volume, and inclusive pages. The articles must be numbered in chronological order (oldest publications first, most recent publications last). In cases where there are multiple publications within a year, they should be listed by alphabetical order. The list should not include papers "in preparation," "submitted," or "under revisions", nor should it include conference proceedings, published abstracts, and book reviews (which need to be listed separately).

Articles published: (Provide a PDF of each peer-reviewed article (PRA) published within the last five years. Other articles may be provided at the applicant's discretion.)

01PRA - Wang J, Dong T, Tang T, Zu G. 99m-Tc-MDP bone scintigraphy for diagnosis of femoral head

necrosis after femoral neck fractures. Jiangsu Med J 1989; 15:194-196.

02PRA - Wang J, Xu L, Dong T. Arthoroscopic examination and surgery of the knee. Jiangsu Med J 1989; 15:579-581.

03PRA - Wang J, Dong T, Liu Z. A histological study of avascular necrosis and repair of the femoral head after femoral neck fractures. Acta Acad Suzhou 1990; 10:263-267.

04PRA - Wang J, Dong T, Tang T. A combination of clinical and research training: A new orthopaedic residency program. Chin J Orthop 1990; 10:308-309.

05PRA - Wang J, Dong T, Liu Z. Pathologic observations of osteoarthritis following avascular necrosis of the femoral head. J Clin Exp Pathol 1991; 7:111-114.

06PRA - Wang J, Dong T, Liu Z. Correlations of intramedullary haemorrhage and avascular necrosis of the femoral head. J Bone Joint Injury 1992; 7:7-10.

07PRA - Sang S, Jian Y, Wang J. The scintigraphic changes in the repair process of experimentally induced avascular necrosis of the femoral head. Chin J Nucl Med 1993; 13:102-104.

304

08PRA - Wang J, Tong T, Chen X, Dong Q, Wong W, Dai M, Chen M. A biomechanical study on the

repairing process of avascular necrosis of the femoral head in dogs. Chin J Surg 1993; 31:374-377.

09PRA - Dong QR, Wang JX, Dong TH. Early diagnosis by scintigraphy of segmental collapse of the femur head following femur neck fracture. J Radiol 1994; 75:423-425.

10PRA - Wang J, Dong Q, Dong T, Wu Y, Sang S. The changes in radionuclide bone imaging during the repair process of the human femoral head necrosis after femoral neck fracture. Chin J Orthop 1994; 14:751-754.

11PRA - Dong Q, Wang J, Dong T. Prediction of segmental collapse of femoral head after femoral neck fracture by scintimetry. Clin J Surg 1994; 32:520-522.

12PRA - Dong Q, Wang JX, Dong T. Early diagnosis by scintigraphy of femur head necrosis after femur neck fracture. Chirurgie 1995; 120:194-197.

13PRA - Wang J, Dong T, Tang T, Zu G. Results of vascularized (muscle) pedicle bone grafts for femoral head necrosis after femoral neck fracture: Three to nine-year follow-up in 87 cases. Acta Acad Med Suzhou 1995; 1:102-109.

14PRA - Dong T, Wang J. Implantation of hydroxyapatite-methyl-methyacrylate in the treatment of avascular necrosis of the femoral head: Experimental study and preliminary clinical application. Acta Acad Med Suzhou 1995; 1:1-8.

15PRA - Dong Q, Wang J, Zheng Z, Dong T, He G. Early diagnosis for segmental collapse of the femoral head after femoral neck fracture by scintimetry. Acta Acad Med Suzhou 1995; 1:117-121.

16PRA - Wang J, Glimcher MJ, Mah J, Zhou HY,Salih E. Expression of bone microsomal casein kinase II, bone sialoprotein, and osteopontin during the repair of calvarial defects. Bone 1998; 22:621-628.

17PRA - Wu Y, Ackerman JL, Chesler DA, Li J, Neer RM, Wang J, Glimcher MJ. Evaluation of bone mineral density using three dimensional solid state phosphorus-31 NMR projection imaging. Calcif Tissue Int 1998; 62:512-518.

18PRA - Wang J, Kennedy JG, Kasser JR, Glimcher MJ, Salih E. Novel biological property of purified native bone sialoprotein in bone repair of a calvarial defect. Orthop Trans 1999; 22:951-952.

19PRA - Wu Y, Chesler DA, Glimcher MJ, Garrido L, Wang J, Jiang HJ, Ackerman JL. Multinuclear solid-state three-dimensional MRI of bone and synthetic calcium phosphates. Proc Natl Acad Sci USA 1999; 96:1574-1578.

20PRA - Wang J, Mah J, Zhou HY, Glimcher MJ, Salih E. Interrelationship Between bone microsomal casein kinase II, bone sialoprotein, osteopontin and mineral accumulation. Orthop Trans 1999; 22:832-833.

21PRA - Wang J, Glimcher MJ. Characterization of matrix-induced osteogenesis in rat calvarial bone defects.

Part I: Differences in the cellular response to demineralized bone matrix implanted in calvarial defects and n subcutaneous sites. Calcif Tissue Int 1999; 65:156-165.

305

22PRA - Wang J, Glimcher MJ. Characterization of matrix-induced osteogenesis in rat calvarial bone defects.

Part II: Origins of bone forming cells. Calcif Tissue Int 1999; 65:486-493.

23PRA - Ranger AM, Gerstenfeld LC, Wang J, Kon T, Bae H, Gravallese EM, Glimcher MJ, Glimcher LH. The Nuclear factor of activated T cells (NFAT) transcription factor NFATp (NFATc2) is a repressor of chondrogenesis. J Exp Med 2000; 191:9-21.

24PRA - Wang J. Spatial orientation of the microscopic elements of cortical repair bone. Clin Orthop 2000; 374:265-277.

25PRA - Wang J, Yang R, Gerstenfeld LC, Glimcher MJ. Characterization of matrix-induced osteogenesis in rat calvarial bone defects. Part III: Gene and protein expression. Calcif Tissue Int 2000; 67:314-320.

26PRA - Dong T, Liu S, Zhu G, Wei W, Wang J. Implantation of hydroxyapatite methylmethacrylate complex in the prevention of degenerative osteoarthritis following osteonecrosis of the femoral head. Chin J Orthop 2002; 22:84-87.

27PRA - Salih E, Wang J (Co-first-author), Mah J, Fluckiger R. Natural variation in the extent of phosphorylation of bone phosphoproteins as a function of in vivo new bone formation induced by demineralized bone matrix in soft tissue and bony environments. Biochem J 2002; 364:465-474.

28PRA - Wang Y, Wang J, Xu Z, Wu S, Lu X, Li G. An experimental study of cranial bone repair elicited by implantation of bone-healing herb extract. Chin J Orthop Traum 2003; 16(6):346-348.

29PRA - Wang J, Glimcher MJ. Spatial differences in the cellular response to demineralized bone matrix implanted in long bone defects of rabbits. Connect Tissue Res 2003; 44(suppl. 1):365-366.

30PRA - MacLean HE, Kim JI, Glimcher MJ, Wang J, Kronenberg HM, Glimcher LH. Absense of transcription factor c-maf causes abnormal terminal differentiation of hypertrophic chondrocytes during endochondral bone development. Dev Biol 2003; 262:51-63.

31PRA - Mah J, Hung J, Wang J, Salih E. The efficacy of various alloplastic bone grafts on the healing of rat calvarial defects. European J Orthodontics 2004; 26: 475-482.

32PRA - Wang J , Zhou HY, Salih E, Xu L, Wunderlich L, Gu X, Hofstaetter JG, Torres M, Glimcher MJ. Site- specific In vivo calcification and osteogenesis stimulated by bone sialoprotein. Calcif Tissue Int 2006; 79:179-189.

33PRA - Hofstaetter JG, Wang J, Yan J, Glimcher MJ. Changes in bone microarchitecture and bone mineral density following experimental osteonecrosis of the hip in rabbits. Cells Tissues Organs 2006; 184:138-147.

34PRA - Xu L, Anderson AL, Q Lu, Wang J (corresponding author). Role of fibrillar structure of collagenous carrier in bone sialoprotein-mediated matrix mineralization and osteoblast differentiation. Biomaterials 2007; 28:750-761.

306

35PRA - Garimella R, Kacena M, Tague S, Wang J, Horowitz M, Anderson HC. Expression of bone

morphogenetic proteins and their receptors in the bone marrow megakaryocytes of GATA-1low

mice: A possible role in osteosclerosis. J Histochem Cytochem 2007; 55:745-52.

36PRA - Garimella R, Tague S, Zhang J, Belibi F, Nahar N, Sun B, Insogna K, Wang J, Anderson HC. Expression and synthesis of bone morphogenetic proteins by osteoclasts: A possible path to anabolic bone remodeling. J Histochem Cytochem 2008; 56:569-77.

37PRA - Hofstaetter JG, Wang J, Yan J, Glimcher MJ. The effects of alendronate in the treatment of experimental osteonecrosis of the hip in adult rabbits. Osteoarthritis Cartilage 2009; 17:362-70.

38PRA - Wang J, Gardner BM, Lu Q, Rodova M, Woodbury BG, Yost JG, Roby KF, Pinson DM, Tawfik O, Anderson HC. Transcription factor NFAT1 deficiency causes osteoarthritis through dysfunction of adult articular chondrocytes. J Pathol 2009; 219:163-72.

39PRA - Wang Q, Wang J, Lu Q, Detamore MS, Berkland C. Injectable PLGA based colloidal gels for zero-order dexamethasone release in cranial defects. Biomaterials 2010; 31:4980-86.

40PRA - Schroeppel JP, Crist JD, Anderson HC, Wang J. Molecular regulation of articular chondrocyte function and its significance in osteoarthritis. Histol Histopathol: Cell Mol Biol 2011; 26:377-94.

41PRA Kramer WC , Hendricks KJ, Wang J. Pathogenetic mechanisms of posttraumatic

osteoarthritis: Opportunities for early intervention. Int J Clin Exp Med 2011; 4:285-98.

42PRA - Rodova M, Lu Q, Li Y, Woodbury BG, Crist JD, Gardner BM,Yost JG, Zhong XB, Anderson HC, Wang J. Nfat1 regulates adult articular chondrocyte function through its age-dependent expression mediated by epigenetic histone methylation. J Bone and Miner Res 2011; 26:1974-86.

43PRA- Nahar NN, Tague SE, Wang J, Danley M, Garimella R, Anderson HC. Histological characterization of bone marrow in ectopic bone, induced by devitalized Saos-2 human osteosarcoma cells. Int J Clin Exp Med 2013; 6:119-25.

44PRA- Kruger TE, Miller AH, Wang J. Collagen scaffolds in bone sialoprotein-mediated bone regeneration.

Scientific World J 2013; Mar 31, E-Pub; doi:10.1155/2013/812718.

45PRA- Kruger TE, Miller AH, Godwin AK, Wang J. Bone sialoprotein and osteopontin in bone metastasis of osteotropic cancers. Crit Rev Oncol Hematol 2013 Sep 7. doi: 10.1016/j.critrevonc.2013.08.013. [Epub ahead of print]

Manuscripts in press: (MIP, provide names of all authors, title, journal, and PDF of manuscript plus evidence of acceptance by journal.)

307

Manuscripts submitted but not yet accepted: (MS, provide names of all authors, title, journal, and PDF of manuscript plus evidence of receipt of manuscript by journal.)

1MS- Zhang M, Lu Q, Crist J, Theleman C, Zhong X, Brandt K, Wang J. Histone methylation-mediated

decrease in NFAT1 expression causes dysfunction of articular chondrocytes in aged mice. Submitted to Arthritis & Rheumatism.

Invited or non-peer-reviewed articles or reviews: (Provide names of all authors, year, title, journal, volume, and pages. If in press, provide documentation and PDF or article/review if published within the last five years.)

Books and book chapters: (Provide names of all authors, year, book title, chapter title, edition, publisher, and pages. If in press, provide documentation and PDF or book/book chapter if published the last five years.)

1) Wang J, Mah J, Glimcher MJ, Salih E. Biochemical changes during new bone formation in the cranial and subcutaneous tissue environments. In: Davidovitch Z and Mah J editors. Biological mechanisms of tooth eruption, resorption and replacement by implants. EBSCO Media, Birmingham, Alabama, 1998. p. 123- 132.

2) Wang J, Dong T. Pathophysiology and pathogenesis of osteonecrosis of the femoral head. In: Zu CX, Chan ZG, Wong GT, and Liao YM editors. Diagnosis and treatment of avascular necrosis of the femoral head: The advanced courses for clinical medicine. Chansao: Hunan Science and Technology Publishing House; 1999. p. 81-88.

3) Wang J, Dong T. Biomechanical changes during the repair process of avascular necrosis of the femoral head. In: Zu CX, Chan ZG, Wong GT, and Liao YM editors. Diagnosis and treatment of avascular necrosis of the femoral head: The advanced courses for clinical medicine. Chansao: Hunan Science and Technology Publishing House; 1999. p. 88-93.

4) Wang J, Dong T. Temporal changes in radionuclide bone imaging during the repair process of the human femoral head necrosis after femoral neck fracture. In: Zu CX, Chan ZG, Wong GT, and Liao YM editors. Diagnosis and treatment of avascular necrosis of the femoral head: The advanced courses for clinical medicine. Chansao: Hunan Science and Technology Publishing House; 1999. p. 108-112.

5) Zhou H-Y, Glimcher MJ, Wang J, Salih E. A novel glycosylated phosphoprotein, osteometrin. In: Goldberg M, Boskey AL, Robinson C, editors. Proc of the Sixth International Conference on the Chemistry and Biology of Mineralized Tissues. Rosemont, IL, Amer Acad Othop Surg, 2000. p.185-188.

6) Wang J, Glimcher MJ. Biomaterial-elicited bone repair and bone remodeling in cranial defects. In: Davidovitch Z and Mah J, editors. Biological mechanisms of tooth eruption, resorption and replacement by implants. EBSCO Media, Birmingham, 2000. p.9-16.

7) Wang J, Zhou H-Y, Salih E, Gu X, Xu L, Hofstaetter JG, Glimcher MJ. Bone sialoprotein-collagen complex elicits mineralization and ossification in a bone defect model. In: Landis W and Sodek J (eds.), Proceeding of 8th International Conference on the Chemistry and Biology of Mineralized Tissues. University of Toronto Press, Toronto, 2004; pp 139-142.

8) Wang J, Dong T. Non-traumatic osteonecrosis of the femoral head. In: Dong T, Lu S, Jie S and Li Z (eds), Surgery of Hip Joint. Zhengzhou University Press, Zhengzhou, 2005; pp391-403.

308

9) Wang J, Kramer WC, Schroeppel JP. Transcriptional regulation of articular chondrocyte function and

its implication in osteoarthritis. In: Rothschild BM (ed), Principles of Osteoarthritis, ISBN 979-953-51-0063-8. InTech, 2012; pp473-496.

Published abstracts: (Provide names of all authors (applicant’s name bolded), year, title, where published, volume, and pages.) If the same work is reflected in a published abstract and a presentation or poster, the work must only be listed only once.

01PA - Wang J, Tong T, Tang T, Zu G. Results of vascularized (muscle) pedicle bone grafts for femoral head necrosis after femoral neck fracture: Three to nine-year follow up in 87 cases. Proceedings of the Seventh Annual Meeting of Orthopaedic Trauma Association, Seattle, 1991; pp115.

02PA - Wang J, Maniwa S, Glimcher MJ. Temporal and spatial independence of bone and cartilage induction by demineralized bone powder in cranial defects and subcutaneous tissue of rats. J Bone Min Res 1994; 9(Supple 1):S181.

03PA - Wu Y, Ackerman JL, Chesler DA, Neer R, Wang J, Glimcher MJ. Three dimensional bone mineral density measurement by solid state phosphorus-31 magnetic resonance imaging. J Bone Min Res 1995; 10(Supple 1):S477.

04PA - Cao Y, Wang J, Perkins M, Vacanti CA. Injectable bone. Trans of 42nd Ann. Meet. Orthop Res Soc 1996;21:619.

05PA - Wang J, Glimcher MJ. Biochemical characterization of matrix-induced osteogenesis in rat cranial bone defects. J Bone Min Res 1996; 11(Supple 1):S406.

06PA - Salih E, Wang J, Glimcher MJ. Factor-independent protein kinase and its correlation with calvarial defect repair induced by demineralized bone. J Bone Min Res 1996; 11(Supple 1):S407.

07PA - Wang J, Mah J, Glimcher MJ, Salih E. Microsomal casein kinase II, bone sialoprotein, osteopontin and their correlation with ossification during calvarial bone repair. J Bone Min Res 1997; 12(Suppl 1):S426.

08PA - Wang J, Yang R, Gerstenfeld L, Glimcher MJ. Molecular characteristics of bone differentiation pathways during matrix-induced osteogenesis in rat cranial defects. J Bone Min Res 1997; 12(Suppl 1):S201.

09PA - Wu Y, Chesler DA, Ackerman JL, Wang J, Glimcher MJ. Solid components of bone mtrix measured by proton magnetic resonance imaging. J Bone Min Res 2001;16(Suppl 1):S463.

309

10PA - Wang J, Salih E, Zhou H-Y, Hofstaetter J, Glimcher MJ. Temporal and spatial characteristics of

bone sialoprotein-elicited in vivo biomineralization and ossification. Trans 49th Ann Meeting, Orthop Res Soc 2003; 28:Paper #143.

11PA - Wang J, Xu L, Wunderlich L, Glimcher LH, Glimcher MJ. Absence of nuclear factor of activated T Cells (NFAT) transcription factor NFATp enhances endochondral ossification during healing of long bone defects. Trans 50th Ann Meeting of Orthop Res Soc 2004; 29:Paper #66.

12PA - Hofstaetter JG, Wang J, Yan J, Glimcher MJ. Changes in bone micro-architecture and mineral density following experimental osteonecrosis of the femoral head in rabbits. Proceedings of the 5th Combined Meeting of the Orthopedic Research Societies of Canada, the U.S., Japan, and Europe, Banff, Canada, 2004; 5:128.

13PA - Wang J, Anderson AL, Lu Q. Cell-type-dependent effects of bone sialoprotein on osteoblast differentiation. J Bone Miner Res 2006; 21(Suppl 1):S346.

14PA - Yost JG, Lu Q, Rodova M, Wang J. Bone Sialoprotein-mediated osteoblast differentiation and mineralization is principally dependent on the intrinsic characteristics of responding cells. J Bone Miner Res 2007; 22(Suppl 1):S267.

15PA - Rodova M, Gardner BM, Lu Q, Yost JG, Wang J. Runx2 and Canonical Wnt signaling cooperatively regulate BMP-induced differentiation pathways of adult dural cells into osteoblasts or chondrocytes. J Bone Miner Res 2008; 23 (Suppl 1):S384.

16PA - Yost JG, Lu Q, Gardner BM, Rodova M, Wang J. Transcription factor NFATp regulates BMP-induced chondrocyte differentiation in a tissue-specific manner. J Bone Miner Res 2008; 23 (Suppl 1):S248.

17PA - Wang J, Rodova M, Gardner BM, Lu Q, Trainor PA, Yost JD. Wnt/β-catenin signaling plays an essential role in bone sialoprotein-elicited osteoblast differentiation and mineralization. Bone 2009; 44(Suppl 1):S158-9.

18PA - Wang J, Gardner BM, Lu Q, Rodova M, Woodbury BG, Yost JG, Li Y, Zhong XB, Roby KF, Pinson DM, Tawfik O, Anderson HC. Transcription factor NFAT1 deficiency causes osteoarthritis. J Bone Miner Res 2009; 24(Suppl 1):S59.

19PA - Rodova M, Lu Q, Li Y, Woodbury BG, Crist JD, Gardner BM, Yost JG, Zhong XB, Anderson HC, Wang J. Dynamic Effects of Nfat1 and Sox9 on Articular Chondrocyte Function Associate with Their Age- Dependent Expression and Epigenetic Histone Modifications. J Bone Miner Res 2010; 25(Suppl 1):S247.

20PA- Wang J, Rodova M, Lu Q, Woodbury BG, Zhong XB, Anderson HC. Nfat1 regulates adult articular chondrocyte function through its age-dependent expression mediated by epigenetic histone methylation. Bone 2011; 48(Suppl 2):S142.

21PA- Wang J, Lu Q, Rodova M, Crist JD. Bone sialoprotein stimulates intramembranous ossification through Wnt/beta-catenin signaling pathway during bone repair. Bone 2011; 48(Suppl 2):S113.

310

22PA- Lu Q, Rodova M, Bernard J, Wang J. Bone sialoprotein and Runx2 are interdependent

during osteogenic differentiation of adult mesenchymal stem cells. J Bone Miner Res 2011; 26(Suppl 1):S448.

23PA- Wang J, Gardner B, Lu Q, Shaath M, Brandt K, Anderson H. Deficiency of NFAT1 transcription factor causes osteoarthritis with alterations in articular cartilage and subchondral bone in adult mice.

Osteoarthritis and Cartilage 2012; 20(Suppl 1):S60.

24PA Lu Q, Furomoto B, Anderson HC, Wang J. Bone and muscle interations during the progression of Nfat1 deficiency-mediated osteoarthritis. J Bone Miner Res 2012; 27(Suppl 1):S168.

25PA Wang J, Lu Q, Shaath MK, Bernard J. Bone sialoprotein is essential for osteoblastic cell differentiation and maturation. J Bone Miner Res 2012; 27(Suppl 1):S279.

26PA Wang J, Lu Q, Yost J, Miller A, Garlich J. Bone sialoprotein stimulates the differentiation of dura- derived osteoprogenitor cells into osteoblasts during cranial bone repair. BoneKey 2013; 10 (Suppl1):S80.

27PA Zhang M, Lu Q, Caldwell K, Crist J, Theleman C, Wang J. Decreased Nfat1 expression contributes to dysfunction of articular chondrocytes in aging mice. J Bone Miner Res 2013; 28(Suppl 1):S195.

28PA Zhang M, Lu Q, Bernard J, Miller A, Wang J. Epigenetic regulation of age-dependent Sox9 expression in mouse articular cartilage. J Bone Miner Res 2013; 28(Suppl 1):S362-363.

Other scholarly publications

3 Presentations and posters in chronological order (oldest first, most recent last)

Oral paper presentations: (Provide names of all authors (applicant’s name bolded), title, sponsoring organization, extent of peer-review, and location and date of presentation.)

Scientific papers presented at national and international meetings: 1) Wang J, Glimcher MJ. Spatial independence of bone and cartilage induction by demineralized bone

matrix. International Conference on Bone Morphogenetic Proteins and Bone Reconstruction, Bordeaux, France, June 15-16, 1995.

2) Wang J, Glimcher MJ. Biomaterial-elicited bone repair and bone remodeling in cranial defects. 3rd

International Conference on Biological Mechanisms of Tooth Movement and Craniofacial Adaptation, Seoul, Korea, October 7-10, 1999.

3) Wang J, Salih E, Zhou H-Y, Hofstaetter J, Glimcher MJ. Temporal and spatial characteristics of bone sialoprotein-elicited in vivo biomineralization and ossification. 49th Annual Meeting of Orthopaedic Research Society, New Orleans, LA, USA, Feb. 2-5, 2003, Paper #143.

4) Wang J. Bone sialoprotein-elicited biomineralization and ossification. The 2nd Wittgenstein

311

Conference: Bone and Cartilage in Health and Disease, Vienna, Austria, October 17-19, 2003.

5) Wang J, Xu L, Wunderlich L, Glimcher LH, Glimcher MJ. Absence of nuclear factor of activated T Cells (NFAT) transcription factor NFATp enhances endochondral ossification during healing of long bone defects. 50th Annual Meeting of Orthopaedic Research Society, The Moscone Convention Center, San Francisco, CA, USA, March 7-10, 2004.

6) Wang J, Zhou HY, Salih E, Gu X, Xu L, Hofstaetter JG, Glimcher MJ. Bone sialoprotein-collagen complex elicits mineralization and ossification in a bone defect model. 8th ICCBMT, Banff, Alberta, Canada, Oct. 17- 22, 2004.

7) Hofstaetter JG, Wang J, Samuel RE, Glimcher MJ. Alendronate in the treatment of experimental osteonecrosis. 51th Annual Meeting of Orthopaedic Reseach Society, Washington DC, USA, Feb. 20-23, 2005.

8) Wang J, Rodova M, Lu Q, Gardner BM. Runx2 and canonical Wnt signaling in osteoblast or chondrocyte differentiation. The 3rd International Congress of Chinese Orthopedic Association Suzhou, China, Nov. 13- 16, 2008.

9) Wang J, Gardner BM, Lu Q, Rodova M, Woodbury BG, Yost JG, Roby KF, Pinson DM, Tawfik O, Anderson HC. Transcription factor NFAT1 deficiency causes osteoarthritis through dysfunction of adult articular chondrocytes. 31st Annual Meeting of the American Society for Bone and Mineral Research, Denver, CO, USA, Mar 11-15, 2009.

10) Wang Q, Wang J, Lu Q, Detamore M, Berkland C. Injectable colloidal gels offer zero-order dexamethasone release and cranial bone defect filling. The 37th Annual Meeting and Exposition of the Controlled Release Society (CRS), Portland, Oregon, USA, July 10-14, 2010.

11) Wang J, Lu Q, Rodova M, Crist JD, Trainor PA. Bone sialoprotein stimulates intramembranous ossification during cranial bone repair. 2011 Annual Meeting of the Orthopaedic Research Society, Long Beach, CA, USA, January 13-16, 2011.

12) Wang J, Anderson HC, Lu Q, Zhang M, Furomoto B, Shaath MK. Deficiency of Nfat1 transcription factor causes osteoarthritis through dysfunction of cells in multiple joint tissues. 2012 International Conference on Orthopedics and Rheumatology, Chicago, IL, USA, August 13-15, 2012.

13) Zhang M, Lu Q, Caldwell KL, Crist JD, Theleman CL, Wang J. Histone Methylation-Mediated Decrease in Nfat1 Expression Contributes to Dysfunction of Articular Chondrocytes in Aging Mice. 2013 Annual Meeting of the Orthopaedic Research Society, San Antonio, TX, USA, January 26-29, 2013.

14) Lu Q, Gardner B, Zhang M, Anderson HC, Wang J. Transcription factor Nfat1 regulates the expression of multiple catabolic and anabolic molecules in articular cartilage. 2nd Joint Meeting of the International Bone and Mineral Society and the Japanese Society for Bone and Mineral Research, Kobe, Japan, May 28 - June 1, 2013.

15) Lu Q, Yost J, Miller A, Wang J. Bone sialoprotein-collagen implants promote cranial bone repair by stimulating the differentiation of osteoprogenitior cells into mature osteoblasts. 8th Combined Meeting of Orthopaedic Research Societies, Venice, Italy, October 13-16, 2013.

Scientific papers presented at local and regional meetings:

1) Wang J. Bone sialoprotein and bone regeneration. Orthopaedic Research Seminar Series for

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Harvard Research Community, Boston, MA, USA, December 3, 2004.

2) Wang J, Gardner BM, Lu Q, Rodova M, Woodbury BG, Yost JG, Roby KF, Pinson DM, Tawfik O, Anderson HC. Transcription factor NFAT1 deficiency causes osteoarthritis through dysfunction of adult articular chondrocytes. Bone Day, University of Missouri at Kansas City School of Dentistry, Sept. 4, 2009.

3) Shaath MK, Wang J. Nfat1 is a critical transcription factor for regulating the function of adult articular chondrocytes. 33rd Annual Student Research Forum, University of Kansas Medical Center, March 31, 2011.

4) Kramer WC, Wang J. Transcription factor Nfat1 and posttraumatic osteoarthritis in mice. 2011 KU Medical Center Resident Research Day, May 5, 2011.

5) Kramer, WC, Wang J. Nfat1 deficiency is a risk factor for the development of posttraumatic osteoarthritis. 2011 Annual Meeting of Kansas Orthopedic Society, Kansas City, KS, Sept. 16, 2011.

6) Garlich J, Wang J. Role of Runx2 transcription factor in bone sialoprotein-mediated osteogenic differentiation. 2012 Annual Student Research Forum, University of Kansas Medical Center, March 29, 2012.

7) Theleman C, Wang J. Nfat1 deficiency causes osteoarthritic subchondral bone changes through pathological differentiation of bone marrow stem cells. 2012 Annual Student Research Forum, University of Kansas Medical Center, March 29, 2012.

8) Caldwell K, Wang J. The role of Nfat1 in the healing of osteochondral defects. 2012 Annual Meeting of Kansas State Orthopedic Society, Wichita, KS, Sept. 14, 2012.

9) Wang J. Epigenetic regulation of Nfat1 expression in articular chondrocytes and its implication in osteoarthritis. Institute for Reproductive Health and Regenerative Medicine. Kansas City, KS, February 20, 2013.

10) Wang J. Molecular regulation of chondrocyte differentiation. Department of Anatomy and Cell Biology Seminar, University of Kansas School of Medicine, Kansas City, KS, May 9, 2013.

Poster presentations in chronological order (oldest first, most recent last): (Provide names of all authors (applicant’s name bolded), title, sponsoring organization, extent of peer-review and location and date of presentation.)

Poster presentations at national and international meetings: 1) Wang J, Tong T, Tang T, Zu G. Results of vascularized (muscle) pedicle bone grafts for femoral head

necrosis after femoral neck fracture: Three to nine-year follow up in 87 cases. Seventh Annual Meeting of Orthopaedic Trauma Association, Seattle, Washington, Oct. 31- Nov. 2, 1991.

2) Wang J, Maniwa S, Glimcher MJ. Temporal and spatial independence of bone and cartilage induction by demineralized bone powder in cranial defects and subcutaneous tissue of rats. 16th Annual Meeting of the American Society for Bone and Mineral Research, Kansas City, MO, Sept. 9-13, 1994.

3) Wu Y, Ackerman JL, Chesler DA, Neer R, Wang J, Glimcher MJ. Three dimensional bone mineral density measurement by solid state phosphorus-31 magnetic resonance imaging. 17th Annual Meeting of the American Society for Bone and Mineral Research, New York, Sept. 9-13, 1995.

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4) Wang J, Glimcher MJ. Biochemical characterization of matrix-induced osteogenesis in rat cranial bone defects. 18th Annual Meeting of the American Society for Bone and Mineral Research, Seattle, Washington, Sept. 7-11, 1996.

5) Salih E, Wang J, Glimcher MJ. Factor-independent protein kinase and its correlation with calvarial defect repair induced by demineralized bone. 18th Annual Meeting of the American Society for Bone and Mineral Research, Seattle, Washington, Sept. 7-11, 1996.

6) Wang J, Mah J, Glimcher MJ, Salih E. Microsomal casein kinase II, bone sialoprotein, osteopontin and their correlation with ossification during calvarial bone repair. 19th Annual Meeting of the American Society for Bone and Mineral Research, Cincinnati, OH, 19th Annual Meeting of the American Society for Bone and Mineral Research, Cincinnati, OH, USA, Sept. 10-14, 1997.

7) Wang J, Yang R, Gerstenfeld L, Glimcher MJ. Molecular characteristics of bone differentiation pathways during matrix-induced osteogenesis in rat cranial defects (peer-reviewed). 19th Annual Meeting of the American Society for Bone and Mineral Research, Cincinnati, OH, USA, Sept. 10-14, 1997.

8) Wu Y, Chesler DA, Ackerman JL, Wang J, Glimcher MJ. Solid components of bone mtrix measured by proton magnetic resonance imaging. 23th Annual Meeting of the American Society for Bone and Mineral Research, Phoenix, AZ, USA, Oct. 12-16, 2001.

9) Hofstaetter JG, Wang J, Yan J, Glimcher MJ. Changes in bone micro-architecture and mineral density following experimental osteonecrosis of the femoral head in rabbits. 5th Combined Meeting of the Orthopedic Research Societies of Canada, the U.S., Japan, and Europe, Banff, Canada, Oct. 2-5, 2004.

10) Wang J, Anderson AL, Lu Q. Cell-type-dependent effects of bone sialoprotein on osteoblast differentiation (peer-reviewed). 28th Annual Meeting of the American Society for Bone and Mineral Research, Philadelphia, PA, USA, Sept. 15-19, 2006.

11) Yost JG, Lu Q, Rodova M, Wang J. Bone Sialoprotein-mediated osteoblast differentiation and mineralization is principally dependent on the intrinsic characteristics of responding cells. Honolulu, Hawaii, USA, Sept. 16-19, 2007.

12) Wang J, Rodova M, Gardner BM, Lu Q, Yost JG. Transcriptional regulation of articular chondrocyte activity. Segal North American Osteoarthritis Workshop, Rush University, Chicago, IL, USA, May 2-4, 2008.

13) Rodova M, Gardner BM, Lu Q, Yost JG, Wang J. Runx2 and Canonical Wnt signaling cooperatively regulate BMP-induced differentiation pathways of adult dural cells into osteoblasts or chondrocytes. 30th Annual Meeting of the American Society for Bone and Mineral Research, Montreal, Canada, Sept. 12-16, 2008.

14) Yost JG, Lu Q, Gardner BM, Rodova M, Wang J. transcription factor NFATp regulates BMP-induced

chondrocyte differentiation in a tissue-specific manner. 30th Annual Meeting of the American Society for Bone and Mineral Research, Montreal, Canada, Sept. 12-16, 2008.

15) Wang J, Rodova M, Gardner BM, Lu Q, Trainor PA, Yost JD. Wnt/β-catenin signaling plays an essential role in bone sialoprotein-elicited osteoblast differentiation and mineralization. 2nd Joint Meeting of the International Bone and Mineral Society and The Australian & New Zealand Bone & Mineral Society, Sydney, Australia, March 21-25, 2009.

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16) Wang Q, Wang J, Detamore M, Berkland C. Injectable colloidal gels for drug delivery to bone defects.

2009 Annual Meeting of the American Association of Pharmaceutical Scientists (AAPS), Los Angeles, California, November 11-14, 2009.

17) Woodbury BW, Rodova M, Lu Q, Gardner BM, Yost JG, Wang J. Runx2 and Wnt/β-catenin signaling cooperatively suppress chondrogenic differentiation during BMP-induced cranial bone repair. 56th

Annual Meeting of the Orthopaedic Research Society, New Orleans, LA, March 6-9, 2010.

18) Rodova M, Lu Q, Li Y, Woodbury B, Crist J, Gardner B, Yost J, Zhong X, Anderson HC, Wang J. Dynamic effects of Nfat1 and Sox9 on articular chondrocyte function associate with their age-dependent expression and epigenetic histone modifications. 32th Annual Meeting of the American Society for Bone and Mineral Research, Toronto, Canada, Oct. 15-19, 2010.

19) Kramer WC, Lu Q, Wang J. Transcription factor Nfat1 deficiency: A risk factor for progression of posttraumatic osteoarthritis in mice. 58th Annual Meeting of the Orthopedic Research Society, San Francisco, CA, Feb. 4-7, 2012.

20) Lu Q, Furomoto B, Anderson HC, Wang J. Bone and muscle interactions during the progression of Nfat1 deficiency-mediated osteoarthritis. American Society for Bone and Mineral Research Topical Meeting: Bone and Skeletal Muscle Interactions, Kansas City, MO, July 17-18, 2012

21) Lu Q, Yost JG, Gardner BM, Wang J. Contribution of the dura to bone sialoprotein-mediated cranial bone regeneration. 2012 International Conference on Orthopedics and Rheumatology, Chicago, IL, USA, August 13-15, 2012.

22) Zhang M, Lu Q, Bernard J, Miller AH, Wang J. Epigenetic regulation of age-dependent Sox9 expression in mouse articular cartilage. 2013 Annual Meeting of the American Society for Bone and Mineral Research, Baltimore, MD, USA, October 4-7, 2013.

23) Zhang M, Lu Q, Caldwell K, Crist J, Theleman C, Wang J. Decreased Nfat1 expression contributes to dysfunction of articular chondrocytes in aging mice. 2013 Annual Meeting of the American Society for Bone and Mineral Research, Baltimore, MD, USA, October 4-7, 2013.

Poster presentations at local and regional meetings:

1) Wang J, Anderson A, Lu Q. Cell-type-dependent effects of bone sialoprotein on osteoblast differentiation. University of Kansas Medical Center Faculty Research Day, Kansas City, KS, November 9, 2006.

2) Rodova M, Gardner BM, Lu Q, Yost JG, Wang J. Runx2 and Canonical Wnt signaling cooperatively

regulate BMP-induced differentiation pathways of adult dural cells into osteoblasts or chondrocytes. University of Kansas Medical Center Faculty Research Day, Kansas City, KS, November 6, 2008.

3) Yost JG, Lu Q, Gardner BM, Rodova M, Wang J. Transcription factor NFATp regulates BMP-

induced chondrocyte differentiation in a tissue-specific manner. University of Kansas Medical Center Faculty Research Day, Kansas City, KS, November 6, 2008.

4) Wang J, Rodova M, Gardner BM, Lu Q, Trainor PA, Yost JG. Wnt/β-catenin signaling plays an essential role

in bone sialoprotein-elicited osteoblast differentiation and mineralization. University of Kansas Medical Center Faculty Research Day, Kansas City, KS, November 3, 2009.

5) Wang J, Woodbury BW, Rodova M, Lu Q, Gardner BM, Yost JG. Runx2 and Wnt/β-catenin signaling

cooperatively suppress chondrogenic differentiation of adult mesenchymal stem cells. University of

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Kansas Medical Center Faculty Research Day, Kansas City, KS, November 12, 2010.

6) Wang J, Lu Q, Yost JG, Miller AH, Garlich J. Bone Sialoprotein stimulates the differentiation of dura-

derived osteoprogenitor cells into osteoblasts during cranial bone repair. Bone and Muscle Day, University of Missouri at Kansas City, School of Dentistry, Kansas City, MO, September 27, 2013.

7) Zhang M, Lu Q, Bernard J, Miller AH, Wang J. Epigenetic regulation of age-dependent Sox9 expression

in mouse articular cartilage. Bone and Muscle Day, University of Missouri at Kansas City, School of Dentistry, Kansas City, MO, September 27, 2013.

8) Zhang M, Lu Q, Caldwell K, Crist J, Theleman C, Wang J. Decreased Nfat1 expression contributes to

dysfunction of articular chondrocytes in aging mice. Bone and Muscle Day, University of Missouri at Kansas City, School of Dentistry, Kansas City, MO, September 27, 2013.

Invited seminars at other universities or institutions: (Provide title, sponsoring organization or institution, and date of presentation.)

1) Cellular and molecular response to osteogenic morphogens implanted in bone defects. Sponsored by School of Dentistry, Dankook University, Chunan City, South Korea, October 9, 1999.

2) Orthopedic tissue engineering. Sponsored by Soochow University Medical School, Suzhou, P. R. China, June 20, 2002.

3) Cellular and molecular biology of bone repair. Sponsored by West Virginia University School of Medicine, Morgantown, WV, May 13, 2004.

4) Genetic engineering in bone and cartilage regeneration. Sponsored by the Orthopedic Training Program, the Ministry of Health, Suzhou, P.R. China, September 14, 2004.

5) In vivo function of bone sialoprotein in mineralization and osteogenesis. Sponsored by School of Dentistry, University of Missouri at Kansas City, Kansas City, MO, June 21, 2006.

6) Advances in molecular orthopedics. Sponsored by the First Affiliated Hospital of Soochow University, Suzhou, P. R. China, December 30, 2007.

7) Tissue-specific response to bone morphogenetic protein. Sponsored by Soochow University Medical School, Suzhou, P. R. China, November 16, 2008.

8) Osteoarthritis: from mouse to man. Sponsored by the First Affiliated Hospital of Soochow University, Suzhou, P. R. China, July 26, 2010.

9) Transcription factor NFAT1 Deficiency and osteoarthritis. Sponsored by Rush University Medical Center, Chicago, IL, March 25, 2011.

10) The role of Wnt and BMP signaling pathways in bone sialoprotein-mediated osteogenesis. Sponsored by Stowers Institute for Medical Research, Kansas City, MO, September 23, 2011.

11) Mechanisms of bone sialoprotein-specific osteogenic action. Sponsored by School of Dentistry, University of Missouri at Kansas City, Kansas City, MO, November 30, 2011.

12) Grant applications for medical research: Opportunities and challenges. Sponsored by Soochow University Medical School, Suzhou, P.R. China, March 9, 2012.

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13) NFAT signaling in bone marrow stem Cells. Sponsored by Stowers Institute for Medical Research,

Kansas City, MO, May 7, 2012.

14) Posttraumatic osteoarthritis: A keynote lecture. Invited by the Organizing Committee of the 2012 International Conference on Orthopedics and Rheumatology, Chicago, IL, USA, August 13, 2012.

15) U.S Department of Defense (DoD) grant applications. Invited by the Orthopaedic Research Society (ORS) and the Orthopaedic Research and Education Foundation (OREF) and presented at the 2013 ORS/OREF Grant Writing Workshop, San Antonio, TX, USA, January 25, 2013.

16) Posttraumatic osteoarthritis: Pathogenesis and novel therapeutic strategies. Invited by the University of Southern California, Keck School of Medicine, Los Angeles, CA, USA, August 9, 2013.

4 Other evidence of scholarship

(includes clinical guidelines, policy documents, contributions to significant position statements by professional organizations, and development of national examinations)

Patents:

Inventor Title of Patent Filing Institution Date Filed Status

Jinxi Wang Prevention and Treatment KUMC 03/19/2009 Issued by USPTO on 9/11/2012, of Osteoarthritis US patent number: 8263546

Description of the Patent: Subjects lacking Nfat1 display osteoarthritis in weight-bearing joints. Osteoarthritic changes associated with Nfat1 deficiency are characterized by articular cartilage degradation, articular chondrocyte proliferation and clustering, progressive articular surface destruction, chondro-osteophyte formation, and exposure of thickened subchondral bone. Methods for screening drug candidates that may be useful for prevention and treatment of Nfat1 deficiency-associated osteoarthritis are presented.

Inventor Title of Patent Filing Institution Date Filed Status

Jinxi Wang Prevention and Treatment KUMC 08/31/2012 Pending, USPTO of Osteoarthritis: Divisional Application

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