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Curriculum vitae

Antonio Gonzalez-Bulnes

Surname: González de Bulnes López Name: Antonio Nationality: Spanish ID card nº: 06998421G. Date of birth: 06/10/1967 Gender: Male

Professional status 2006, Senior Scientist (Científico Titular) Institution: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA

(National Institute of Agricultural and Food Research, Madrid, Spain) Department: Animal Reproduction Address: Avda. Puerta de Hierro s/n. 28040-Madrid E-mail: bulnes@inia.es

Professional status (other positions)

Executive Secretary of the INIA-PHII Scientific Ethic Committee Executive Secretary of the INIA-CISA Scientific Ethic Committee Executive Secretary of the INIA Biohazard Committee Member of the Regulatory Board of the INIA Scientific Ethic Committees Member of the Scientific Ethic Committee of the Public Research Organizations (OPIs) of the Ministry of Economy and Competitiveness

Education Ph.D. 1997. Reproductive Physiology. Universidad Complutense, Madrid, Spain M.V.D. 1991. Animal Medicine. Universidad Complutense, Madrid, Spain

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Previous research and teaching activities

Previous research experience

1. 2001-06, Researcher. INIA, Department of Animal Reproduction (Madrid, Spain). 2. 2000, Postdoctoral Fellow (INIA) at INIA Department of Animal Reproduction, and

University of Nottingham, United Kingdom. 3. 1998-2000, Research Contract at INIA, Department of Animal Reproduction (Madrid,

Spain). 4. 1997-98 Postdoctoral Fellow (INIA) at CIATA (Asturias, Spain) and University of

Edinburgh, United Kingdom. 5. 1992-96, PhD grant at INIA, Department of Animal Production (Madrid, Spain).

Financed research projects 1. Sharing advances in genetic engineering and phenotyping of non-rodent mammals to

develop predictive animal models for translational medicine (SALAAM). COST Action (BM1308) European Union. 2014-2017.

2. The relative roles of metabolic imbalance and oxidative stress on developmental

programming of the phenotype in Iberian pigs. MINECO. 2014-2016.

3. Epigenetics and periconception environment (EPICONCEPT). COST Action (FA1201) European Union. 2013-2016.

4. Influences of prenatal nutritional programming on growth, adipogenesis and

reproductive performance of Iberian pigs. MCINN. 2011-2013. 5. Effects of the supply of antioxidants on reproductive activity and efficiency of sheep

under hypobaric hypoxia in Chilean highlands. Continuation. AECID. 2011. 6. Effects of the supply of antioxidants on reproductive activity and efficiency of sheep

under hypobaric hypoxia in Chilean highlands. AECID. 2010. 7. Effect of nutrition on intramuscular fat and reproductive features in Iberian pigs. CDTI.

2010-2013. 8. Study of catch-up growth in the Iberian pig and its relationship with different metabolic

hormones. ITACyL. 2009-2010. 9. Maternal Interaction with Gametes and Embryos (GEMINI). COST Action (FA0702)

European Union. 2008-2011, 10. Hormonal and cellular characterization of processes determining reproductive

performance in Iberian sows. ITACyL. 2007-2009. 11. Application of assisted reproduction techniques and conservation of genetic

resources in Iberian sows. INIA. 2007-2009.

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12. Application of an animal model based on adult sheep for the study of the effects of

the (2-etilhexil) phthalate (DEHP). UCM. 2007-2008. 13. Effect of cactus (Opuntia ficus) feeding on reproductive yields of sheep and goats

under traditional management in Tunisia. AECI. 2007. 14. Effect of fat supplementation, protein, exciter aminoacids and beta-carotene on

reproductive performance of goats. URUZA-UACH, INIA, UAAAAN-UL, FMVYZ-UAZ. 2006-2010.

15. Effect of maternal gene expression in embryo peri-implantational survival in sheep

under different oestrus synchronization treatments. CICYT. 2005-2007.

16. Adaptation and application of superovulatory techniques for production and conservation of embryos from Rubia del Molar y Negra de Colmenar breeds. INIA. 2004-2006.

17. Application of embryo conservation techniques under field conditions in MOET

programs in Manchega sheep. UCLM. 2004.

18. Treatment with GnRH antagonists during protocols of ovarian stimulation in sheep. UCM. 2003.

19. Identification of ovarian factors limiting production and conservation of in vitro sheep

embryos. MCYT. 2003-2004.

20. Prolactin. Cellular basis of its regulatory function in ovine follicular development. UCM. 2003.

21. Optimization of fertility yields after induced ovulation and artificial insemination in

goats. INIA. 2002-2004.

22. Regulation of follicular development. MRC-UK. 2002.

23. Effects of the use of a single depot injection of GnRH antagonist on follicular function and superovulatory yields in sheep. OCDE. 2002.

24. Sheep preimplantation: expression pattern of estrogens, progesterone and growth

factors in the genital tract and their relationship with embryo viability. CICYT. 2001-2004.

25. Systemic and intraovarian factors responsible of the selection of the ovulatory follicle

and nuclear and cytoplasm maturation of the oocyte in small ruminants. MCYT. 2001-2006.

26. Development of the technology for goat clonation. Application to feral and domestic

goats. Continuation. INIA. 2001-2003.

27. Development of the technology for goat clonation. Application to feral and domestic goats. INIA. 2000-2001.

28. Identification of determinant factors in the production and freezing of small ruminant

embryos in different pre-implantational stages.2000-2004.

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29. Integration of reproductive technologies for a maximum yield in the production of small ruminant embryos. INIA. 2000-2002.

30. Transfer of technologies for genetic selection and reproduction of Malagueña and

Murciana-Granadina goat breeds. FEDER. 1999-2002.

31. Location and expression of prolactin receptor in the ovine ovary during the estrous cycle and the seasonal anoestrus. UCM. 1999-2000.

32. Genetic and environmental differentiation in the control of seasonal reproductive

activity in domestic (Ovis aries) and feral (Ovis gmelini musimon) small ruminants. INIA. 1998-2000.

33. Improvement of artificial insemination of Murciano-Granadina goats and

characterization of its cheese. INIA. 1997-2000.

34. Improvement of the efficiency of transfer and freezing of sheep and goat embryos produced in vivo and in vitro. INIA. 1997-1999.

35. Study of the interaction pasture x animal in different mountain vegetable landscapes.

INIA. 1995-1998.

36. Production and conservation of sheep and goat embryos. CICYT. 1994-1997.

37. Breeding of Cashmere goats in Spain. INIA. 1993-1997.

38. Influence of ovarian status, secretion of ß-endorphins and FSH on the ovulation rate in sheep. INIA. 1993-1995.

39. Improvement of superovulatory yields and efficiency of embryo freezing in sheep.

CICYT. 1991-1993.

SCI publications

1. Parraguez VH, Diaz F, Cofre E, Urquieta B, De Los Reyes M, Astiz S, Gonzalez-Bulnes A. Fertility of a high-altitude sheep model is compromised by deficiencies in both preovulatory follicle development and plasma LH availability. Reprod. Dom. Anim. 2015 (in press).

2. Meza-Herrera C, Gonzalez-Bulnes A, Kridli R, Mellado M, Arechiga-Flores C,

Salinas H, Luginbuhl J. Neuroendocrine, metabolic and genomic cues signalling the onset of puberty in females. Reprod Domest Anim. 2015 (in press).

3. Torres-Rovira L, Gonzalez-Bulnes A, Succu S, Spezzigu A, Manca ME, Leoni GG, Sanna M, Pirino S, Gallus M, Naitana S, Berlinguer F. Predictive value of antral follicle count and anti-Mullerian hormone for follicle and oocyte developmental competence during the early prepubertal period in a sheep model. Reprod. Fertil. Develop. 2015 (in press).

4. Segura J, Calvo L, Óvilo C, González-Bulnes A, Olivares A, Cambero MI, López-Bote CJ. Alternative method for intramuscular fat analysis using common laboratory equipment. Meat Sci. 2015, 103, 24–27.

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5. Hernandez F, Elvira L, Fernández B, Egea M, Gonzalez-Bulnes A, Gonzalez-Martin JV, Astiz S. Effects of intramammary antibiotic therapy during the dry period on the performance of Lacaune dairy sheep under intensive management. J. Dairy Res. 2015, 82, 95–101.

6. Gonzalez-Bulnes A, Ovilo C, Astiz S. Transgenerational inheritance in the offspring

of pregnant women with metabolic syndrome. Current Pharm. Biotech. 2014, 15, 13-23.

7. Gonzalez-Bulnes A, Astiz S, Ovilo C, Lopez-Bote CJ, Sanchez-Sanchez R, Perez-

Solana ML, Torres-Rovira L, Ayuso M, Gonzalez J. Early-postnatal changes in adiposity and lipids profile by transgenerational developmental programming in swine with obesity/leptin resistance. J. Endocrinol. 2014, 223, M17–M29.

8. Astiz S, Gonzalez-Bulnes A, Astiz I, Barbero A, Perez-Solana ML, Garcia-Real, I. Advanced onset of puberty after metformin therapy in swine with thrifty genotype Exp Physiol 2014, 99, 1241–1252.

9. Barbero A, Astiz S, Ovilo C, Lopez-Bote CJ, Perez-Solana M, Ayuso M, Garcia-

Real I, Gonzalez-Bulnes A. Prenatal programming of obesity in a swine model of leptin resistance: modulatory effects of controlled postnatal nutrition and exercise. J Develop Origin Health Dis. 2014, 5, 248–258.

10. Barbero A, Garcia-Real I, Astiz S, Ayuso M, Lopez-Bote CJ, Gonzalez-Bulnes A.

Feasibility of MRI and selection of adequate regions of interest for longitudinal studies of growth and fatness in swine models of obesity. Diagn Interv Imaging. 2014, 95, 839—847.

11. Ovilo C, Gonzalez-Bulnes A, Benitez R, Ayuso M, Barbero A, Perez-Solana ML, Barragan C, Astiz S, Fernandez A, Lopez-Bote C. Prenatal programming in an obese swine model: sex-related effects of maternal energy restriction on morphology, metabolism and hypothalamic gene expression. Br. J. Nutr. 2014; 111: 735–746.

12. Torres-Rovira L, Astiz S, Gonzalez-Añover P, Pallares P, Perez-Garnelo S, Perez-Solana M, Sanchez-Sanchez R, Gonzalez-Bulnes A. Intake of high saturated-fat diets disturbs steroidogenesis, lipid metabolism and development of obese-swine conceptuses from early-pregnancy stages. J Steroid Biochem Mol Biol 2014; 139: 130-137.

13. Astiz S, Gonzalez-Bulnes A, Sebastian F, Fargas O, Cano I, Cuesta P. Maternal

aging affects life performance of progeny in a Holstein dairy cow model. J Develop Origin Health Dis. 2014, 5, 374–384.

14. Manca ME, Manunta ML, Spezzigu A, Torres-Rovira L, Gonzalez-Bulnes A, Pasciu

V, Piu P, Leoni GG, Succu S, Chesneau D, Naitana S, Berlinguer F. Melatonin deprival modifies follicle and corpora lutea growth dynamics in a sheep model. Reproduction 2014, 147, 885–895.

15. Bruno-Galarraga MM, Cueto M, Gibbons AE, Pereyra-Bonnet F, Catalano R, Gonzalez-Bulnes A. Repeatability of superovulatory response to successive FSH treatments in Merino sheep. Small Rumin. Res. 2014, 120, 84–89.

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16. Sakly C, Rekik M, Ben Salem I, Lassoued N,Gonzalez-Bulnes A, Ben Salem H. Reproductive response of fat-tailed Barbarine ewes subjected to short-term nutritional treatments including spineless cactus (Opuntia ficus-indica f. inermis) cladodes. J Anim Physiol Anim Nutr (Berl). 2014; 98:43-49.

17. Barbero A, Astiz S, Lopez-Bote C, Perez-Solana ML, Ayuso M, Garcia-Real I, Gonzalez-Bulnes A. Maternal malnutrition and offspring sex determine juvenile obesity and metabolic disorders in a swine model of leptin resistance. PLoS ONE 2013; 8: e78424.

18. Herreros MA, Encinas T, Torres-Rovira L, Garcia-Fernandez RA, Flores JM, Ros JM, Gonzalez-Bulnes A. Exposure to the endocrine disruptor di(2-ethylhexyl)phthalate affects female reproductive features by altering pulsatile LH secretion. Environ. Toxicol. Pharmacol. 2013; 36: 1141-1149.

19. Gonzalez-Bulnes A, Ovilo C, Lopez-Bote C, Astiz S, Ayuso M, Perez-Solana ML,

Sanchez-Sanchez R, Torres-Rovira L. Fetal and early-postnatal developmental patterns of obese-genotype piglets exposed to prenatal programming by maternal over- and undernutrition. Endocr Metabol Immune Disord Drug Targets, 2013; 13: 240-249.

20. Lassoued N, Rekik M, Gonzalez-Bulnes A, Ben Salemb I, Tounsi A. Prolific strains

of Barbarine sheep are characterized byincreased ovulation rate due to extended period of ovulatoryfollicle recruitment and co-dominance effects. Small Rumin Res. 2013; 114: 134-139.

21. Torres-Rovira L, Tarrade A, Astiz S, Mourier E, Perez-Solana ML, de la Cruz P, Gomez-Fidalgo E, Sanchez-Sanchez R, Chavatte-Palmer P, Gonzalez-Bulnes A. Sex and breed-dependent organ development and metabolic responses in fetuses from lean and obese/leptin resistant swine. PLoS ONE 2013; 8: e66728.

22. Gonzalez-Bulnes A, Astiz S, Sanchez-Sanchez R, Perez-Solana ML, Gomez-

Fidalgo E. Maternal diet-induced obesity in swine with leptin resistance modifies puberty and pregnancy outputs of the adult offspring. J Develop Origin Health Dis 2013; 4: 290–295.

23. Torres-Rovira L, Gonzalez-Añover P, Astiz S, Caro A, Lopez-Bote C, Ovilo C,

Pallares P, Perez-Solana ML, Sanchez-Sanchez R, Gonzalez-Bulnes A. Effect of an obesogenic diet during the juvenile period on growth pattern, fatness and metabolic, cardiovascular and reproductive features of swine with obesity/leptin resistance. Endocr Metabol Immune Disord Drug Targets, 2013; 13: 143-151.

24. Astiz S, Gonzalez-Bulnes A, Perez-Solana ML, Sanchez-Sanchez R, Torres-

Rovira L. In vitro release of ovarian progesterone is decreased during the oestrous cycle and pregnancy of swine with obesity/leptin resistance. Reprod Domest Anim. 2013; 48: e44–e48.

25. Arias-Álvarez M, García-García RM, Lorenzo P, Gutiérrez-Adán A, Sakr OG, González-Bulnes A, Rebollar PG. Embryo gene expression in response to maternal supplementation with glycogenic precursors in the rabbit. Anim. Reprod Sci. 2013; 142: 173– 182.

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26. Parraguez VH, Urquieta B, de los Reyes M, Gonzalez-Bulnes A, Astiz S, Muñoz A. Steroidogenesis in sheep pregnancy with intrauterine growth retardation by high-altitude hypoxia: effects of maternal altitudinal status and antioxidant treatment. Reprod Fertility Develop 2013; 25: 639–645.

27. Parraguez VH, Urquieta B, Perez L, Castellaro G, de los Reyes M, Torres-Rovira

L, Aguado-Martínez A, Astiz S, Gonzalez-Bulnes A. Fertility in a high-altitude environment is compromised by luteal dysfunction: the relative roles of hypoxia and oxidative stress. Reprod Biol Endocrinol 2013; 11: 24.

28. Herreros MA, Gonzalez-Bulnes A, Iñigo-Nuñez S, Contreras-5 Solis I, Ros JM,

Encinas T. Toxicokinetics of di(2-ethylhexyl) phthalate (DEHP) and its effects on the luteal function in sheep. Reprod Biol. 2013; 13: 66-74.

29. Meza-Herrera CA, Vargas-Beltran F, Tena-Sempere M, González-Bulnes A,

Macias-Cruz U, Veliz-Deras FG. Short-term betacarotene-supplementation positively affects ovarian activity and serum insulin concentrations in a goat model. J Endocrinol Invest. 2013; 36: 185-189.

30. Ruiz-González I, Sánchez M, García-Fernández R, García-Palencia P, Sánchez B,

González-Bulnes A, Flores J. Different influence of ovine estrus synchronization treatments on caruncular early angiogenesis. Histol Histopathol. 2013;28: 373-383.

31. Gonzalez-Bulnes A, Ovilo C, Lopez-Bote CJ, Astiz S, Ayuso M, Perez-Solana ML, Sanchez-Sanchez R, Torres-Rovira L. Gender-specific early postnatal catch-up growth after intrauterine growth retardation by food restriction in swine with obesity/leptin resistance. Reproduction. 2012; 144: 269-278.

32. Gonzalez-Bulnes A, Torres-Rovira L, Ovilo C, Astiz S, Gomez-Izquierdo E,

Gonzalez-Añover P, Pallares P, Perez-Solana ML, Sanchez-Sanchez R. Reproductive, endocrine and metabolic feto-maternal features and placental gene expression in a swine breed with obesity/leptin resistance. Gen Comp Endocrinol. 2012; 176: 94-101.

33. Torres-Rovira L, Gonzalez-Añover P, Pallares P, Perez-Solana ML, Astiz S,

Gomez-Izquierdo E, Sanchez-Sanchez R, Gonzalez-Bulnes A. The interaction between ovulation rate and embryo survival in determining prolificacy of different strains of obese swine with gene polymorphisms for leptin receptors. Anim Prod Sci 2012; 52: 58–63.

34. Torres-Rovira L, Astiz S, Caro A, Lopez-Bote C, Ovilo C, Pallares P, Perez-Solana

ML, Sanchez-Sanchez R, Gonzalez-Bulnes A. Diet-induced swine model with obesity/leptin resistance for the study of metabolic syndrome and type 2 diabetes. Sci World J. 2012; 2012: 510149.

35. Gonzalez-Bulnes A, Ovilo C. Genetic basis, nutritional challenges and adaptive

responses in the prenatal origin of obesity and type-2 diabetes. Curr Diabetes Rev. 2012; 8: 144-54.

36. Gonzalez-Añover P, Vigo E, Encinas T, Torres-Rovira L, Pallares P, Gomez-

Izquierdo E, Sanchez-Sanchez R, Mallo F, Gonzalez-Bulnes A. Prepuberal evolution of plasma leptin levels in gilts of thrifty genotype (Iberian pig) and lean commercial crosses (Large White×Landrace). Res Vet Sci. 2012; 93: 100-102.

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37. Gonzalez-Bulnes A, Astiz S, Encinas T, Gonzalez-Añover P, Perez-Solana M, Sanchez-Sanchez R, Torres-Rovira L, Tresguerres JA. Characterization of a distinctive pattern of periovulatory leptin secretion and its relationship with ovulation rate and luteal function in swine with obesity/leptin resistance. Peptides. 2012; 37: 290–293.

38. Abecia JA, Forcada F, González-Bulnes A. Hormonal control of reproduction in

small ruminants. Anim Reprod Sci. 2012; 130: 173-179. 39. Berlinguer F, Gonzalez-Bulnes A, Spezzigu A, Contreras-Solis I, Succu S,

McNeilly AS, Naitana S, Leoni GG. Effect of aging on follicular function may be relieved by exogenous gonadotropin treatment in a sheep model. Reproduction. 2012; 144: 245-255.

40. Gómez-Fernández J, Gómez-Izquierdo E, Tomás C, González-Bulnes A,

Sánchez-Sánchez R, de Mercado E. Inclusion of seminal plasma in sperm cryopreservation of Iberian pig. Anim Reprod Sci. 2012; 130: 82-90.

41. Rekik M, Gonzalez-Bulnes A, Lassoued N, Ben Salem H, Tounsi A, Ben Salem I.

The cactus effect: an alternative to the lupin effect for increasing ovulation rate in sheep reared in semi-arid regions?. J Anim Physiol Anim Nutr (Berl). 2012; 96: 242-249

42. Ruiz-González I, Sánchez M, García-Fernández R, García-Palencia P, Sánchez B,

Letelier C, González-Bulnes A, Flores J. Endometrial expression of IFNAR-1 and oxytocin receptor (OTR) is not improved by prostaglandin analogues when compared to progestagens in ewes. Reprod Domest Anim. 2012; 47: 274–280

43. Ruiz-González I, Sánchez MA, García-Palencia P, Sánchez B, García-Fernández

RA, González-Bulnes A, Flores JM. Differences in uterine immunoexpression of PR, ERα and OTR when comparing prostaglandin- to progestagen-based protocols for ovine estrus synchronization. Anim Reprod Sci. 2012; 133: 93-100.

44. Berlinguer F, Gonzalez-Bulnes A, Contreras-Solis I, Spezzigu A, Torres-Rovira L, Succu S, Naitana S, Leoni GG. Glucogenic supply increases oocyte developmental competence in sheep. Reprod Fertility Develop 2012; 24: 1055–1062.

45. Abecia JA, Forcada F, González-Bulnes A. Pharmaceutical control of reproduction

in sheep and goats. Vet Clin North Am Food Anim Pract. 2011; 27: 67-79. 46. Cuervo-Arango J, García-Roselló E, García-Muñoz A, Valldecabres-Torres X,

Martínez-Ros P, González-Bulnes A. The effect of a single high dose of PGF2α administered to dairy cattle 3.5 days after ovulation on luteal function, morphology, and follicular dynamics. Theriogenology. 2011; 76: 1736-1743.

47. Cueto MI, Gibbons AE, Pereyra-Bonnet F, Silvestre P, González-Bulnes A.

Effects of season and superovulatory treatment on embryo yields in fine-wool Merinos maintained under field conditions. Reprod Domest Anim.2011;46:770-775.

48. Gonzalez-Añover P, Encinas T, Sanz E, Letelier CA, Torres-Rovira L, de Mercado

E, Pallares P, Sanchez-Sanchez R, Gonzalez-Bulnes A. Preovulatory follicle dynamics and ovulatory efficiency in sows with thrifty genotype and leptin resistance due to leptin receptor gene polymorphisms (Iberian pig). Gen Comp Endocrinol. 2011; 170: 200-206.

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49. Gonzalez-Añover P, Encinas T, Torres-Rovira L, Pallares P, Muñoz-Frutos J,

Gomez-Izquierdo E, Sanchez-Sanchez R, Gonzalez-Bulnes A. Ovulation rate, embryo mortality and intrauterine growth retardation in obese swine with gene polymorphisms for leptin and melanocortin receptors. Theriogenology. 2011; 75: 34-41.

50. Gonzalez-Añover P, Encinas T, Torres-Rovira L, Sanz E, Pallares P, Ros J,

Gomez-Izquierdo E, Sanchez-Sanchez R, Gonzalez-Bulnes A. Patterns of corpora lutea growth and progesterone secretion in sows with thrifty genotype and leptin resistance due to leptin receptor gene polymorphisms (Iberian Pig). Reprod Domest Anim. 2011; 46: 1011-1016.

51. Gonzalez-Bulnes A, Pallares P, Ovilo C. Ovulation, Implantation and Placentation

in Females with Obesity and Metabolic Disorders: Life in the Balance. Endocr Metabol Immune Disord Drug Targets. 2011; 11: 285-301.

52. Hernandez F, Elvira L, Gonzalez-Martin JV, Gonzalez-Bulnes A, Astiz S. Influence of age at first lambing on reproductive and productive performance of Lacaune dairy sheep under an intensive management system. J Dairy Res. 2011; 4: 1-8.

53. Letelier CA, Contreras-Solis I, García-Fernández RA, Sánchez MA, García-

Palencia P, Sánchez B, Ariznavarreta C, Tresguerres JA, Flores JM, Gonzalez-Bulnes A. Effects of oestrus induction with progestagens or prostaglandin analogues on ovarian and pituitary function in sheep. Anim Reprod Sci. 2011; 126: 61-69.

54. Letelier CA, Sanchez MA, Garcia-Fernandez RA, Sanchez B, Garcia-Palencia P,

Gonzalez-Bulnes A, Flores JM. Deleterious effects of progestagen treatment in VEGF expression in corpora lutea of pregnant ewes. Reprod Domest Anim. 2011; 46: 481-488.

55. Meza-Herrera CA, Hernández-Valenzuela LC, González-Bulnes A, Tena-Sempere

M, Abad-Zavaleta J, Salinas-Gonzalez H, Mellado M, Veliz-Deras F. Long-term beta-carotene supplementation enhances serum insulin concentrations without effect on the onset of puberty in the female goat. Reprod Biol 2011; 11: 236-249.

56. Meza-Herrera CA, Torres-Moreno M, López-Medrano JI, González-Bulnes A, Veliz FG, Mellado M, Wurzinger M, Soto-Sanchez MJ, Calderón-Leyva MG. Glutamate supply positively affects serum release of triiodothyronine and insulin across time without increases of glucose during the onset of puberty in female goats. Anim Reprod Sci. 2011; 125: 74-80.

57. Muñoz-Frutos J, Encinas T, Pallares P, Torres-Rovira L, Gonzalez-Añover P,

Gomez-Izquierdo E, Sanchez-Sanchez R, Gonzalez-Bulnes A. Developmental competence of antral follicles and their oocytes after gonadotrophin treatment of sows with gene polymorphisms for leptin and melanocortin receptors (Iberian pig). J Assist Reprod Genet. 2011; 28: 437-443.

58. Pallares P, Perez-Solana ML, Torres-Rovira L, Gonzalez-Bulnes A. Phenotypic

characterization by high-resolution three-dimensional magnetic resonance imaging evidences differential effects of embryo genotype on intrauterine growth retardation in NOS3-deficient mice. Biol Reprod. 2011; 84: 866-871.

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59. Rosales-Nieto CA, Gamez-Vazquez HG, Gudino-Reyes J, Reyes-Ramirez EA, Eaton M, R. Stanko L, Meza-Herrera CA, Gonzalez-Bulnes A. Nutritional and metabolic modulation of the male effect on the resumption of ovulatory activity in goats. Anim Prod Sci. 2011; 51: 115–122.

60. Torres-Rovira L, Pallares P, Gonzalez-Añover P, Perez-Solana ML, Gonzalez-

Bulnes A. The effects of age and reproductive status on blood parameters of carbohydrate and lipid metabolism in Iberian obese sows. Reprod Biol. 2011; 11: 165-171.

61. Torres-Rovira L, Pallares P, Vigo E, Gonzalez-Añover P, Sanchez-Sanchez R,

Mallo F, Gonzalez-Bulnes A. Plasma leptin, ghrelin and indexes of glucose and lipid metabolism in relation to the appearance of post-weaning oestrus in Mediterranean obese sows (Iberian pig). Reprod Domest Anim. 2011; 46: 558-560.

62. Arias-Alvarez M, García-García RM, Torres-Rovira L, González-Bulnes A, Rebollar

PG, Lorenzo PL. Influence of leptin on in vitro maturation and steroidogenic secretion of cumulus-oocyte complexes through JAK2/STAT3 and MEK 1/2 pathways in the rabbit model. Reproduction. 2010;139(3):523-32.

63. Arias-Alvarez M, García-García RM, Torres-Rovira L, González-Bulnes A, Rebollar

PG, Lorenzo PL. Influence of hormonal and non-hormonal estrus synchronization methods on follicular and oocyte quality in primiparous lactating does at early postpartum period. Theriogenology. 2010;73(1):26-35.

64. Gonzalez-Añover P, Encinas T, Gomez-Izquierdo E, Sanz E, Letelier CA, Torres-

Rovira L, Pallares P, Sanchez-Sanchez R, Gonzalez-Bulnes A. Advanced onset of puberty in gilts of thrifty genotype (Iberian pig). Reprod Domest Anim. 2010;45(6):1003-7.

65. Gonzalez-Bulnes A, Pallares P, Vazquez MI. Ultrasonographic imaging in small

ruminant reproduction. Reprod Domest Anim. 2010 Jun;45 Suppl 2:9-20. 66. Herreros MA, Gonzalez-Bulnes A, Iñigo-Nuñez S, Letelier C, Contreras-Solis I,

Ros-Rodriguez JM, Encinas T. Pregnancy-associated changes in plasma concentration of the endocrine disruptor di(2-ethylhexyl) phthalate in a sheep model. Theriogenology. 2010 15;73(2):141-6.

67. Letelier C, García-Fernández RA, Contreras-Solis I, Sanchez MA, Garcia-Palencia

P, Sanchez B, Gonzalez-Bulnes A, Flores JM. Progestogen treatments for cycle management in a sheep model of assisted conception affect the growth patterns, the expression of luteinizing hormone receptors, and the progesterone secretion of induced corpora lutea. Fertil Steril. 2010 1;93(4):1308-15.

68. Meza-Herrera CA, Ross TT, Hallford DM, Hawkins DE, Gonzalez-Bulnes A. High

periconceptional protein intake modifies uterine and embryonic relationships increasing early pregnancy losses and embryo growth retardation in sheep. Reprod Domest Anim. 2010 45(4):723-8.

69. Pallares P, Garcia-Fernandez RA, Criado LM, Letelier CA, Fernandez-Toro JM,

Esteban D, Flores JM, Gonzalez-Bulnes A. Substantiation of ovarian effects of leptin by challenging a mouse model of obesity/type 2 diabetes. Theriogenology. 2010;73(8):1088-95.

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70. Pallares P, Gonzalez-Bulnes A. The effect of embryo and maternal genotypes on prolificacy, intrauterine growth retardation and postnatal development of Nos3-knockout mice. Reprod Biol. 2010;10:241-8.

71. Salem IB, Rekik M, Gonzalez-Bulnes A, Lassoued N, Kraïem K. Differences in

preovulatory follicle dynamics and timing of preovulatory LH surge affect fertility of maiden sheep reared in semi-arid extensive conditions. Anim Reprod Sci. 2010;117: 60-66.

72. Sosa C, Gonzalez-Bulnes A, Abecia JA, Forcada F, Meikle A. Short-term

undernutrition affects final development of ovulatory follicles in sheep synchronized for ovulation. Reprod Domest Anim. 2010;45(6):1033-8.

73. Vázquez MI, Blanch MS, Alanis GA, Chaves MA, Gonzalez-Bulnes A. Effects of

treatment with a prostaglandin analogue on developmental dynamics and functionality of induced corpora lutea in goats. Anim Reprod Sci. 2010 ;118:42-7.

74. Flores-Najera MJ, Meza-Herrera CA. Echavarría G, Villagomez E, Iñiguez L, Salinas H, Gonzalez-Bulnes A. Influence of nutritional and socio-sexual cues upon reproductive efficiency of goats exposed to the male effect under extensive conditions. Anim Prod Sci 2010; 50: 897–901.

75. Iñigo-Nuñez S, Herreros MA, Encinas T, Gonzalez-Bulnes A. Estimated daily intake of pesticides and xenoestrogenic exposure by fruit consumption in the female population from a Mediterranean country (Spain). Food Control 2010; 21: 471–477.

76. Arellano-Rodriguez, G., Meza-Herrera, C.A., Rodriguez-Martinez, R., Dionisio-

Tapia, R., Hallford, D.M., Mellado, M., Gonzalez-Bulnes, A. Short-term intake of beta-carotene supplemented diets enhances ovarian function and progesterone synthesis in an animal model. Journal of Animal Physiology and Animal Nutrition 2009; 93:710-715.

77. Berlinguer, F., Leoni, G., Succu, S., Spezzigu, A., Madeddu, M., Satta V.,

Bebbere, D., Contreras-Solís, I., Gonzalez-Bulnes, A., Naitana, S. Exogenous melatonin positively influences follicular dynamics, oocyte developmental competence and blastocyst output in a goat model. Journal of Pineal Research 2009; 46: 383–391

78. Contreras-Solis, I., Vazquez, B.; Diaz, T., Letelier, C.A., Lopez-Sebastian, A.,

Gonzalez-Bulnes, A. Ovarian and endocrine responses in tropical sheep treated with reduced doses of cloprostenol. Anim. Reprod. Sci. 2009; 114: 384–392

79. Contreras-Solis, I., Vazquez, B.; Diaz, T., Letelier, C.A., Lopez-Sebastian, A.,

Gonzalez-Bulnes, A. Efficiency of estrous synchronization in tropical sheep by combining short-interval cloprostenol-based protocols and ‘‘male effect’’. Theriogenology 2009, 71: 1018–1025

80. Gonzalez-Añover, P., Encinas, T., Gomez-Izquierdo, E., Sanz, E., Sánchez-

Sánchez, R., Gonzalez-Bulnes, A. Accuracy of in vivo and ex vivo ultrasonographic evaluation of ovarian follicles and corpora lutea in sows. Theriogenology 2009; 71: 1433–1439.

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81. Letelier, C.A., Contreras-Solis, I., Garcia-Fernandez, R.A., Ariznavarreta, C., Tresguerres, J.A.F., Flores, J.M., Gonzalez-Bulnes, A. Ovarian follicular dynamics and steroid production in sheep given sponges impregnated with 20 or 40 mg of fluorogestone acetate. Theriogenology 2009; 71: 676–682

82. Pallares, P., Fernandez-Valle, M.E., Gonzalez-Bulnes, A. In vivo virtual histology

of mouse embryogenesis by ultrasound biomicroscopy and magnetic resonance imaging. Reproduction, Fertility and Development 2009; 21: 283–292

83. Pallares, P., Gonzalez-Bulnes, A. A new method for induction and synchronization

of estrus and fertile ovulations in mice by using exogenous hormones. Lab Animals 2009; 43: 295–299

84. Pallares, P., Gonzalez-Bulnes, A. The use of ultrasound imaging for early

diagnosis of pregnancy and determination of litter size in the mouse. Laboratory Animals 2009; 43: 91–95

85. Pallares, P., Letelier, C.A., Gonzalez-Bulnes, A. Progress toward ‘‘in vivo virtual

histology’’ of ovarian follicles and corpora lutea by ultrasound biomicroscopy. Fertility and Sterility 2009; 91: 624-626.

86. Urrutia-Morales J, Meza-Herrera CA, Escobar-Medina FJ, Gamez-Vazquez HG,

Ramirez-Andrade BM, Diaz-Gomez MO, Gonzalez-Bulnes A. Relative roles of photoperiodic and nutritional cues in modulating ovarian activity in goats. Reproductive Biology 2009; 9: 283-294.

87. Contreras-Solis, I., Diaz, T., Lopez, G., Caigua, A., Lopez-Sebastian, A.,

Gonzalez-Bulnes, A. Systemic and intraovarian effects of corpus luteum on follicular dynamics during oestrous cycle in hair breed sheep. Animal Reproduction Science 2008 85:162-165.

88. Meza-Herrera CA, Hallford DM, Ortiz JA, Cuevas RA, Sanchez JM, Salinas H,

Mellado M, Gonzalez-Bulnes A. Body condition and protein supplementation positively affect periovulatory ovarian activity by non LH-mediated pathways in goats. Animal Reproduction Science. 2008 106:412-420.

89. Contreras-Solis, I., Gómez-Brunet, A., Encinas, T., Gonzalez-Bulnes, A., Santiago-

Moreno, J., López-Sebastian, A. Influence of vehicle on kinetics of exogenous progesterone administered either by subcutaneous and intramuscular routes to sheep. Research in Veterinary Science 2008 85:162-165

90. Fernandez-Moro, D., Veiga-Lopez, A., Ariznavarreta, C., Tresguerres, J.A.F.,

Encinas, T., Gonzalez-Bulnes, A. Preovulatory follicle development in goats following oestrus synchronization with progestagens or prostaglandins. Reproduction in Domestic Animals 2008 43:9-14.

91. Gómez-Brunet, A., Santiago-Moreno, J., del Campo,A., Malpaux, B., Chemineau,

P., Tortonese, D., Gonzalez-Bulnes, A., López-Sebastian, A. Endogenous circannual cycles of ovarian activity and changes in prolactin and melatonin secretion in wild and domestic female sheep maintained under a long day photoperiod. Biology of Reproduction 2008 78:552-562.

92. Gonzalez-Bulnes A, Veiga-Lopez A. Evidence of intraovarian follicular dominance

effects during controlled ovarian stimulation in sheep model. Fertility and Sterility. 2008 89:1507-13

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93. Herreros, M.A., Iñigo, S., Sanchez-Perez, E., Encinas, T., Gonzalez-Bulnes, A.

Contribution of fish consumption to heavy metals exposure in women of childbearing age from a Mediterranean country (Spain). Food and Chemical Toxicology 2008 46:1591-1595.

94. Letelier, C., Gonzalez-Bulnes, A., Herve, M., Correa, J., Pulido, R. Enhancement

of ovulatory follicle development in maiden sheep by short-term supplementation with steam-flaked corn. Reproduction in Domestic Animals 2008 43:222-227.

95. Letelier, C., Mallo, F., Encinas, T., Ros, J.M., Gonzalez-Bulnes, A. Glucogenic

supply increases ovulation rate by modifying follicle recruitment and subsequent development of preovulatory follicles without effects on ghrelin pathway. Reproduction 2008 136:65-72

96. Pallares P, Garcia-Fernandez RA, Criado LM, Letelier CA, Esteban D, Fernandez-

Toro JM, Flores JM, Gonzalez-Bulnes A. Disruption of the endothelial nitric oxide synthase affects ovulation, fertilization and early embryo survival in a knockout mouse model. Reproduction 2008; 136:573-579.

97. Pallares P, Gonzalez-Bulnes A. Feasibility of ultrasound biomicroscopy for non-

invasive, sequential and translational assessment of ovarian features in rodent models. Reproductive Biology 2008; 8:279-284.

98. Pallares P, Gonzalez-Bulnes A. Intrauterine growth retardation in endothelial nitric

oxide synthase-deficient mice is established from early stages of pregnancy. Biology of Reproduction 2008, 78:1002-1006.

99. Pallares P, Gonzalez-Bulnes A. Non-invasive ultrasonographic characterization of

phenotypic changes during embryo development in non-anesthetized mice of different genotypes. Theriogenology 2008, 70:44-52.

100. Veiga-Lopez A, Dominguez V, Souza CJ, Garcia-Garcia RM, Ariznavarreta C,

Tresguerres JA, McNeilly AS, Gonzalez-Bulnes A. Features of follicle-stimulating hormone-stimulated follicles in a sheep model: keys to elucidate embryo failure in assisted reproductive technique cycles. Fertility and Sterility. 2008 89: 1328-1337.

101. Veiga-Lopez, A., Encinas, T., McNeilly, A.S., Gonzalez-Bulnes, A. Timing of

preovulatory LH surge and ovulation in superovulated sheep are affected by follicular status at start the FSH treatment. Reproduction in Domestic Animals 2008 43:92-98.

102. Gibbons, A., Bonnet, F.P., Cueto, M.I., Catala, M., Salamone, D.F., Gonzalez-

Bulnes, A. Procedure for maximizing oocyte harvest for in vitro embryo production in small ruminants. Reproduction in Domestic Animals 2007, 42: 423-426

103. Berlinguer, F., Gonzalez-Bulnes, A., Succu, S., Leoni, G., Mossa, F., Bebbere, D.,

Ariznavarreta, C., Tresguerres, J.A.F., Veiga-Lopez, A., Naitana, S. Effects of progestagens on follicular growth and oocyte developmental competence in FSH-treated sheep. Domestic Animal Endocrinology 2007, 32: 303–314

104. Garcia-Palencia, P.; Sanchez, M.A.; Nieto, A.; Vilar, M.P.; Gonzalez, M.; Veiga-

Lopez, A.; Gonzalez-Bulnes, A.; Flores, J.M. Sex steroid receptor expression in the oviduct and uterus of sheep with estrus synchronized with progestagen or prostaglandin analogues. Animal Reproduction Science 2007, 7: 25-35.

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105. Gonzalez-Añover, P., Encinas, T., Veiga-Lopez, A., Ammoun, I., Contreras, I.,

Ros, J.M., Ariznavarreta, C., Tresguerres, J.A.F., Gonzalez-Bulnes, A. Effects of breed on follicular dynamics and oestradiol secretion during the follicular phase in sheep. Reproduction in Domestic Animals 2007, 42: 29-33.

106. Gomez-Brunet, A.; Santiago Moreno, J.; Montoro, V.; Garde, J.; Pons, P.;

Gonzalez-Bulnes, A.; Lopez, A. Reproductive performance and progesterone secretion in estrus-induced Manchega ewes treated with hCG at the time of AI. Small Ruminant Research 2007, 71: 117–122.

107. Meza-Herrera, C., Ross, T., Hallford, D., Hawkins, D., Gonzalez-Bulnes, A. Effects

of body condition and protein supplementation on LH secretion and luteal function in sheep. Reproduction in Domestic Animals 2007, 42:461-465.

108. López-Sebastian, A., Gonzalez-Bulnes, A., Carrizosa, J.A., Urrutia, B., Díaz-Delfa,

C., Santiago-Moreno, J., Gómez-Brunet, A. New estrus synchronization and artificial insemination protocol for goats based on male exposure, progesterone and cloprostenol during the non breeding season. Theriogenology 2007, 68:1081-1087.

109. Gonzalez Añover P, Gonzalez-Bulnes A, Veiga-Lopez A, Garcia-Garcia RM, McNeilly AS, Encinas T. Effects of growth hormone and gonadotrophin releasing hormone antagonists on ovarian follicle growth in sheep. Journal of Veterinary Pharmacology 2006, 29: 373-377.

110. Veiga-Lopez, A., Gonzalez-Bulnes, A., Tresguerres, J.A.F., Dominguez, V.,

Ariznavarreta, C., Cocero, M.J. Causes, characteristics and consequences of anovulatory follicles in superovulated sheep. Domestic Animal Endocrinology 2006, 30: 76-87.

111. Garcia-Garcia, R.M., Gonzalez-Bulnes, A., Dominguez, V., Veiga-Lopez, A.,

Cocero, M.J. Survival of frozen-thawed sheep embryos cryopreserved at cleavage stages. Cryobiology 2006, 52: 108-113.

112. Cueto, M.; Gibbons, A.; Alberio, R.; Taddeo, H.; Gonzalez-Bulnes, A. Timing of

emergence of ovulatory follicles in polyovulatory goats. Animal Reproduction Science. 2006, 91: 27

113. Garcia-Garcia, R.M., Dominguez, V., Gonzalez-Bulnes, A., Veiga-Lopez, A.,

Cocero, M.J. Effect of embryo developmental stage and culture conditions on number and quality of ovine in vitro produced blastocysts. Zygote. 2006, 14: 181-187

114. Berlinguer, F., Gonzalez-Bulnes, A., Succu, S., Leoni, G., Veiga-Lopez, A., Mossa,

F., Garcia-Garcia, R.M., Cocero, M.J., Naitana, S. GnRH antagonists enhance follicular growth in superovulated sheep but affect developmental competence of oocytes collected by ovum pick-up. Theriogenology. 2006, 65:1099-1109

115. Ammoun, I.; Encinas T.; Veiga-Lopez, A.; Ros, J.M.; Contreras, I.; Gonzalez-

Añover, P.; Cocero, M.J.; McNeilly, A.S.; Gonzalez-Bulnes, A. Effects of breed on kinetics of ovine FSH and ovarian response in superovulated sheep. Theriogenology 2006, 66: 896-905.

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116. Santiago Moreno, J.; Gómez-Brunet, A.; Toledano-Díaz, A.; Picazo, R.A.; Gonzalez-Bulnes, A.; Lopez-Sebastián, A. Seasonal Endocrine Changes and Breeding Activity in Mediterranean Wild Ruminants. Reproduction in Domestic Animals 2006, 41: 72-81.

117. Gonzalez-Bulnes, A.; Carrizosa, J.A.; Urrutia, B.; Lopez-Sebastian, A. Oestrous

behaviour and development of preovulatory follicles in goats induced to ovulate using the male effect with and without progesterone priming. Reproduction, Fertility and Development, 2006, 18: 745–750

118. Veiga-Lopez, A., Cocero, M.J., Dominguez, V., McNeilly, A.S., Gonzalez-Bulnes,

A. Follicular wave status at the beginning of the FSH treatment modifies reproductive features in superovulated sheep. Reproductive Biology 2006, 6: 243-264

119. Garcia R, Sanchez M, Letelier C, Garcia Palencia P, Sanchez B, Vilar M,

Gonzalez-Bulnes A, Flores J. OC11 Changes in Fibroblast Growth Factor-2 in Ovine Follicle Development. Reproduction in Domestic Animals 2006, 41: 105.

120. Gonzalez-Bulnes, A., Souza, C.J.H., Scaramuzzi, R.J.; Campbell, B.K., Baird, D.T.

Long-term suppression of reproductive function by a single dose of gonadotropin-releasing hormone antagonists in a sheep model. Fertility and Sterility 2006, 86: 1121-1128.

121. Santiago-Moreno, J.; Gómez-Brunet. A. A. Toledano-Díaz, A.; González-Bulnes,

A.; Picazo, R.A.; López-Sebastián, A. Influence of age on the relationship between annual changes in horn growth rate and prolactin secretion in the European mouflon (Ovis gmelini musimon). Animal Reproduction Science 2005, 85: 251-261.

122. González-Bulnes, A.; López-Sebastián, A.; García-García, R. M.; Veiga-López, A.;

Souza, C. J. H.; McNeilly, A.S. Restoration of endocrine and ovarian function after stopping GnRH antagonist treatment in goats. Theriogenology. 2005, 63: 83-91.

123. Gonzalez-Bulnes, A.; Díaz-Delfa, C.; Garcia-Garcia, R.M.; Urrutia, B.; Carrizosa,

J.A.; Lopez-Sebastian, A. Origin and fate of preovulatory follicles after induced luteolysis at different stages of the luteal phase in goats. Animal Reproduction Science. 2005, 86: 237-245.

124. Santiago-Moreno, J.; Gomez-Brunet, A.; Gonzalez-Bulnes, A.; Toledano-Dıaz, A.;

Malpaux, B.; Lopez-Sebastian, A. Differences in reproductive pattern between wild and domestic rams are not associated with inter-specific annual variations in plasma prolactin and melatonin concentrations. Domestic Animal Endocrinology. 2005, 28: 416-429.

125. García-García, R.M.; González-Bulnes, A.; Domínguez, V.; Veiga-López, A.;

Cocero, M.J. Culture of early stage ovine embryos to blastocyst enhances survival rate after cryopreservation. Theriogenology. 2005, 63: 2233-2242.

126. Veiga-López, A.; González-Bulnes, A.; García-García, R.M.; Domínguez, V.;

Cocero, M.J. Role of previous ovarian status in ovulation rate and early embryo development in response to superovulatory FSH treatments in sheep. Theriogenology. 2005, 63: 1973-1983.

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127. Gonzalez-Bulnes, A.; Veiga-Lopez, A.; Garcia, P.; Garcia-Garcia, R.M.; Ariznavarreta, C.; Sanchez, M.A.; Tresguerres, J.A.F.; Cocero, M.J.; Flores, J.M. Effects of progestagens and prostaglandin analogues on ovarian function and embryo viability in sheep. Theriogenology. 2005, 63: 2523-2534.

128. Gonzalez-Bulnes, A.; Berlinguer, F.; Cocero, M.J.; García-García, R.M.; Leoni, G.;

Naitana, S.; Rosati, I.; Succu, S.; Veiga-Lopez, A. Induction of the presence of corpus luteum during superovulatory treatments enhances in vivo and in vitro blastocysts output in sheep. Theriogenology. 2005, 64: 1392-1403.

129. Lopez-Alonso, A.; Encinas, T.; García-García, R.M.; Veiga-López, A.; Ros, J.M.;

McNeilly, A.S.; Gonzalez-Bulnes, A. Administration of single short-acting doses of GnRH antagonist modifies pituitary and follicular function in sheep. Domestic Animal Endocrinology. 2005, 29: 476-487.

130. Lopez-Alonso, A.; Encinas, T.; Veiga-López, A.; García-García, R.M.; Cocero,

M.J.; Ros, J.M.; McNeilly, A.S.; Gonzalez-Bulnes, A. Follicular growth, endocrine response and embryo yields in sheep superovulated with FSH after pretreatment with a single short-acting dose of GnRH antagonist. Theriogenology. 2005, 64: 1833-1843.

131. Santiago-Moreno, J.; Gonzalez-Bulnes,A.; Gómez-Brunet,A.; Toledano-Diaz,A.;

Lopez-Sebastian, A. Prediction of gestational age by transrectal ultrasonographic measurements in the mouflon (Ovis gmelini musimon). Journal of Zoo and Wildlife Medicine 2005, 36(3): 457-462.

132. González-Bulnes, A.; Díaz-Delfa, C.; Urrutia, B.; Carrizosa, J.A.; López-

Sebastián, A. Ultrasonographic screening of ovulatory process in goats. Small Ruminant Research. 2004, 52: 165-168.

133. González-Bulnes, A.; Santiago-Moreno J.; García-García, R. M.; Souza, C. J. H.;

López-Sebastián, A.; McNeilly, A.S. Effect of GnRH antagonists treatment on gonadotrophin secretion, follicular development and inhibin A secretion in goats. Theriogenology. 2004, 61: 977-985

134. González-Bulnes, A.; García-García, R. M.; Carrizosa, J.A.; Urrutia, B.; Souza, C.

J. H.; Santiago-Moreno J.; Cocero, M.J.; López-Sebastián, A.; McNeilly, A.S. Plasma inhibin A determination at start superovulatory FSH treatments predictive for embryo outcome in goats. Domestic Animal Endocrinology 2004, 26: 259-266.

135. Santiago-Moreno, J.; López-Sebastián, A.; del Campo, A.; González-Bulnes, A.;

Picazo, R.A.; Gómez-Brunet. A. Effect of constant-release melatonin implants and prolonged exposure to a long day photoperiod on prolactin secretion and hair growth in mouflon (Ovis gmelini musimon). Domestic Animal Endocrinology 2004, 26: 303-314.

136. González-Bulnes, A.; Souza, C. J. H.; Campbell, B.K.; Baird, D.T. Systemic and

intraovarian effects of dominant follicles on ovine follicular growth. Animal Reproduction Science 2004, 84: 107-119.

137. González-Bulnes, A.; Souza, C. J. H.; Campbell, B.K.; Baird, D.T. Effect of Ageing

on Hormone Secretion and Follicular Dynamics in Sheep with and without the Booroola Gene (FecB). Endocrinology 2004, 145: 2858-2864.

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138. González-Bulnes, A.; Baird, D.T.; Campbell, B.K.; Cocero, M.J.; García-García, R.M.; Inskeep, E.K.; López-Sebastián, A.; McNeilly, A.S.; Santiago-Moreno, J.; Souza, C.J.H.; Veiga-López, A. Multiple factors affecting the efficiency of MOET in sheep and goats. Reproduction, Fertility and Development, 2004: 16, 421-435.

139. Souza, C.J.H.; González-Bulnes, A.; Campbell, B.K.; McNeilly, A.S.; Baird, D.T.

Mechanisms of action of the principal prolific genes and their application to sheep production. Reproduction, Fertility and Development, 2004: 16, 395-401.

140. González-Añover, P.; Encinas, T.; García-García, R.M.; Veiga-López, A.;

Cocero, M.J.; McNeilly, A.S.; González-Bulnes, A. Ovarian response in sheep superovulated after pre-treatment with growth hormone and GnRH antagonists is weakened by failures in oocyte maturation. Zygote. 2004, 12: 301-304.

141. Picazo, R.A.; García Ruiz, J.P.; Santiago Moreno, J.; Gonzalez-Bulnes, A.; Muñoz,

J.; Silván, G.; Lorenzo, P.L.; Illera, J.C. Cellular localization and changes in expression of prolactin receptor isoforms in sheep ovary throughout the estrous cycle. Reproduction. 2004, 128: 245-253.

142. González-Bulnes, A.; Carrizosa, J.A.; Díaz-Delfa, C.; García-García, R.; Urrutia,

B.; Santiago-Moreno J.; Cocero, M.J.; López-Sebastián, A. Effects of ovarian follicular status on superovulatory response of dairy goats to FSH treatments. Small Ruminant Research. 2003, 48: 9-14

143. González-Bulnes, A.; García-García, R.M.; Santiago-Moreno J.; Domínguez, V.;

López-Sebastián, A.; Cocero, M.J. Reproductive season affects inhibitory effects from large follicles on superovulatory response in ewes. Theriogenology. 2003, 60: 281-288

144. González-Bulnes, A.; García-García, R. M.; Castellanos, V.; Santiago-Moreno, J.;

Ariznavarreta, C.; Domínguez, V.; López-Sebastián, A.; Tresguerres, J. A. F.; Cocero, M. J. Influence of maternal environment on the number of transferable embryos obtained in response to superovulatory FSH treatments in ewes. Reproduction, Nutrition, Development 2003, 43: 17-28

145. Santiago-Moreno, J.; Gómez-Brunet, A.; González-Bulnes, A.; Malpaux, B.;

Chemineau, P.; Pulido-Pastor, A.; López-Sebastián, A. Seasonal ovulatory activity and plasma prolactin concentrations in Spanish ibex (Capra pyrenaica hispanica) maintained in captivity. Reproduction, Nutrition, Development 2003, 43: 217-224.

146. González- Bulnes A.; Santiago Moreno, J.; Cocero M.J.; Souza C.J.H.; Groome

N.P.; García-García R.M.; López Sebastián, A.; Baird D.T. Measurement of inhibin A and follicular status predicts the response of ewes to superovulatory FSH treatments. Theriogenology. 2002, 57: 1263-1272

147. González-Bulnes, A.; García-García, R. M.; Santiago-Moreno J.; López-Sebastián,

A.; Cocero, M. J. Effects of follicular status on superovulatory response in ewes is influenced by presence of corpus luteum at first FSH dose. Theriogenology. 2002, 58: 1607-1614

148. González-Bulnes, A.; García-García, R. M.; Souza, C. J. H.; Santiago-Moreno J.;

López-Sebastián, A.; Cocero, M. J.; Baird, D. T. Patterns of follicular growth in superovulated ewes and influence on endocrine and ovarian response. Reproduction in Domestic Animals 2002, 37: 357-361.

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149. González-Bulnes, A.; Santiago Moreno, J.; García García, R.M.; del Campo, A.;

Gómez Brunet, A.; López Sebastián, A. Origin of the preovulatory follicle in Mouflon sheep (Ovis gmelini musimon) and effect on growth of remaining follicles during the follicular phase of estrous cycle. Anim. Reprod. Sci. 2001; 65: 265-272.

150. Santiago Moreno, J.; González-Bulnes, A.; Gómez-Brunet, A.; López-Sebastián,

A.; Tortonese, D.J. The timing of the onset of puberty, extension of the breeding season and length of post-partum anoestrus in the female European Mouflon (Ovis gmelini musimon). Journal of Zoo and Wildlife Medicine. 2001, 32: 230-235

151. Santiago Moreno, J.; González de Bulnes, A.; Gómez-Brunet, A.; Cocero M.J.; del

Campo, A.; García-García R.M.; López-Sebastián, A. Procedure for successful interspecific embryo transfer between mouflon (Ovis gmelini musimon) and Spanish Merino sheep (Ovis Aries). Journal of Zoo and Wildlife Medicine. 2001, 32: 336-341

152. Santiago Moreno, J. López-Sebastián,A.; González de Bulnes, A.; Gómez-Brunet,

A.; Chemineau, P. Seasonal changes in ovulatory activity, plasma prolactin and melatonin concentration in Mouflon (Ovis gmelini musimon) and Manchega (Ovis aries) ewes. Reproduction, Nutrition and Development. 2000; 40: 421-430.

153. Santiago-Moreno, J.; González-Bulnes A.; Gómez Brunet, A.; del Campo, A.; Picazo, R.; López Sebastián, A. Nocturnal variation of prolactin secretion in the Mouflon (Ovis gmelini musimon) and domestic sheep (Ovis aries): seasonal changes. Animal Reproduction Science. 2000; 64 (3-4): 211-219.

154. Santiago Moreno, J.; Gómez Brunet, A.; González de Bulnes, A.; López

Sebastián, A. Effect of season on the resumption of pstpartum cyclic ovarian activity in Mouflon (Ovis gmelini musimon) and Manchega ewe (Ovis aries). Small Ruminant Research. 2000, 36: 69-74

155. Santiago Moreno, J.; Gómez Brunet; González de Bulnes, A.; Villar, D.; López

Sebastián, A. Attainment of puberty in the European Mouflon (Ovis gmelini musimon) and the domestic Manchega ewe (Ovis aries). Reproduction in Domestic Animals. 2000 35: 49-52.

156. González de Bulnes, A.; Santiago Moreno, J.; Gómez Brunet; López Sebastián, A.

Relationship between ultrasonographic assessment of the corpus luteum and plasma progesterone concentration during the oestrous cycle in monovular ewes. Reproduction in Domestic Animals. 2000; 35: 65-68.

157. Picazo, R.A.; González de Bulnes, A.; Gómez Brunet, A.; del Campo, A.;

Granados, B.; Tresguerres, J.; López Sebastián, A. Effects of bromocriptine administration during the follicular phase of the oestrous cycle on prolactin and gonadotrophins secretion and follicular dynamics in Merino monovular ewes. Journal of Reproduction and Fertility. 2000 120 (1): 177-186.

158. Santiago Moreno, J.; González de Bulnes, A.; Gómez-Brunet, A.; López-

Sebastián, A. Effect of birth date on body weight, scrotal circumference and horn dimension growth in captive juvenile Mouflon (Ovis gmelini musimon) rams. Game and Wildlife Sciences. 2000; 17(3): 179-187

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159. González de Bulnes, A.; Santiago Moreno, J.; Cocero, M.J.; López Sebastián, A. Effects of FSH commercial preparations and follicular status on follicular growth and superovulatory response to in Spanish Merino ewes. Theriogenology. 2000; 54 (7): 1055-1064.

160. González de Bulnes, A.; Santiago Moreno, J.; Gómez Brunet, A.; Inskeep, E.K.;

Townsend, E.C.; López Sebastián, A. Follicular dynamics during the oestrous cycle in dairy goats. Animal Science. 1999, 68(3), 547-554.

161. López Sebastián, A.; González de Bulnes, A.; Santiago Moreno, J.; Gómez

Brunet, A.; Townsend, E.C.; Inskeep, E.K. Effects of follicular status at treatment on follicular development and ovulation in response to FSH in Spanish Merino ewes. Theriogenology. 1999, 52: 505-514.

162. González de Bulnes, A.; Osoro, K.; López Sebastián, A. Ultrasonographic

assessment of the ovarian response in eCG-treated goats. Small Ruminant Research. 1999, 34: 65-59

163. González de Bulnes, A.; Santiago Moreno, J.; López Sebastián,A. Estimation of

fetal development in Manchega dairy ewes by transrectal ultrasonographic measurements. Small Ruminant Research. 1998, 27(3), 243-250.

164. Gonzalez de Bulnes, A.; García-Fernández, P.; Mayenco Aguirre, A.M.; Sánchez-

Muela, M. Ultrasonographic imaging of canine mammary tumours. The Veterinary Record. 1998, 143, 687-689

165. López Sebastián, A.; González de Bulnes, A.; Santiago Moreno, J.; Gómez

Brunet, A.; Townsend, E.C.; Inskeep, E.K. Patterns of follicular development during the oestrous cycle in monovular Merino del Pais ewes. Animal Reproduction Science. 1997, 48, 279-291.

166. Meana, A.; Luzón-Peña, M.; Santiago Moreno, J.; De Bulnes, A.; Gómez Bautista,

M. Natural infection by gastrointestinal and bronchopulmonary nematodes in Mouflons (Ovis Musimon) and their response to Netobimin treatment. Journal of Wildlife Disease. 1996, 32(1), 39-43.

167. Gómez Bautista, M.; Luzón-Peña, M.; Santiago Moreno, J.; De Bulnes, A.; Meana,

A. Coccidial infection in Mouflon, Ovis Musimon, in Central Spain. Journal of Wildlife Disease. 1996, 32(1), 125-129.

Participation in international scientific meetings. Conferences 1. Embryo viability and offspring development in Iberian pigs. II Conference FATTY

PIG – Science and Utilization. Herceghalom (Hungría), 20-22 November 2013. 2. Actualización en sistemas de manejo reproductivo. XXXVIII Congreso Nacional de

la SEOC. Malaga, 18-20 September 2013. 3. Estrategias sostenibles de manejo reproductivo en pequeños rumiantes. 2012

Reunión Bianual sobre Reproducción Animal. Universidad Autónoma del Estado de México y Universidad Autónoma de Tamaulipas. Temascaltepec (Mexico), 4-5 October 2012.

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4. Protocolos de Superovulación en Ovinos. Simposio Internacional de Biotecnologia Reproductiva en Bovinos, Pequeños Rumiantes y Bufalos. Bogotá (Colombia), 16-17 October 2012.

5. Importancia y Manejo de la Receptora de Embriones Ovinos. Simposio

Internacional de Biotecnologia Reproductiva en Bovinos, Pequeños Rumiantes y Bufalos. Bogotá (Colombia), 16-17 October 2012.

6. Genetic, metabolic and nutritional factors affecting reproductive physiology of fatty

pig breeds. Studies on the Iberian pig. FATTY PIG – Science and Utilization. Herceghalom (Hungary), 17-18 November 2011.

7. In vivo imaging of reproductive features in biomedicine animal models. 16th

International Congress on Animal Reproduction. Budapest (Hungary), 12-17 July 2008.

8. State of the art in assisted reproduction in small ruminants. XVI International

Scientific and Professional Congress of the Mediterranean Federation for Health and Production of Ruminants. Zadar (Croatia). 22-26 April 2008.

9. Dinamica follicolare: reclutamento, selezione e dominanzia. Nuove acquisizioni e

future oportunità nella reproduzione dei ruminanti. Sassari (Italy). 14 September 2007.

10. Avanços na TE em pequenos ruminantes. XXI Reunião Anual da SBTE. Costa do

Sauípe (Brasil). 23-26 August 2007. 11. Tecnologia de reprodução assistida em pequenos ruminantes. XXI Reunião Anual

da SBTE. Costa do Sauípe (Brazil). 23-26 August 2007. 12. Consideraciones en el manejo de machos en programas de inseminación artificial.

III Jornadas de Investigación en Reproducción Animal. Barquisimeto. (Venezuela). 6-9 March 2006.

13. Uso de la bioestimulación para la inducción de celos en ovinos y caprinos. III

Jornadas de Investigación en Reproducción Animal. Barquisimeto. (Venezuela). 6-9 March 2006.

14. Métodos hormonales y de manejo para la sincronización de ovulaciones en

programas de inseminación artificial. III Jornadas de Investigación en Reproducción Animal. Barquisimeto. (Venezuela). 6-9 March 2006.

15. Estado actual de las técnicas de superovulación y obtención de embriones en

ovinos y caprinos. III Jornadas de Investigación en Reproducción Animal. Barquisimeto. (Venezuela). 6-9 March 2006.

16. Influencia de los factores ováricos en la respuesta ovárica y la viabilidad

embrionaria en protocolos de superovulación en ovinos. Diseño de protocolos alternativos. III Jornadas de Investigación en Reproducción Animal. Barquisimeto. (Venezuela). 6-9 March 2006.

17. Estado actual de las técnicas de conservación y transferencia de embriones ovinos.

III Jornadas de Investigación en Reproducción Animal. Barquisimeto. (Venezuela). 6-9 March 2006.

21

18. Tecnologías de Reproducción en la Hembra. Congreso Internacional en Ciencias Veterinarias. Puebla (México) 21-23 November 2005.

19. Ovarian follicular dynamics and dominance effects in sheep. VI Congresso Società

Italiana di Fisiología Veterinaria. Stintino (Italy), 2-4 June 2005. 20. Multiple factors affecting the efficiency of multiple ovulation and embryo transfer in

sheep and goats. Reproduction in Small Ruminants. Colonia (Uruguay), 5-6 August 2004.

21. Mechanisms of action of the principal prolific genes and their application to sheep

production. Reproduction in Small Ruminants. Colonia (Uruguay), 5-6 August 2004. 22. Fatores que afetam a eficiencia da ovulaçao múltipla e transferencia de embrioes

em ovinos e caprinos. Embrapa Clima Temperado. Pelotas (Brasil), 2 August 2004. 23. Estado actual y tendencias futuras de las técnicas de reproducción asistida en

rumiantes. Asociación Mexicana de Médico Veterinarios Especialistas en Bovinos. Ciudad de México (México), 18 September 2003.

24. Reproductive characterization in the female mouflon (Ovis gmelini musimon). IV

International Mouflon Symposium. Nuoro (Italy), 10-12 October 2002. 25. Variability in the response to the photoperiodic cue for the regulation of circannual

reproductive rhythms of the wild and domestic sheep. 6th Annual Conference of the European Society for Domestic Animal Reproduction. Parma (Italy), 12-14 September 2002.

26. Superovulación, producción y transferencia de embriones. II Seminario Internacional en Reproducción Animal y Producción de Leche y de Carne Xochilmico (Mexico), 28 February - 1 March 2002.

27. Biotécnicas aplicadas a la reproducción de pequeños rumiantes. III Simposio

Cearense de Ciencia Animal. Fortaleza-Ceará, Brazil, 3-6 Septiembre 2001. 28. Ovarian follicular dynamics during the oestrous cycle and superovulation in sheep

and goats. 4th Annual Conference of the European Society for Domestic Animal Reproduction. Prague, Czech Republic, 23-25 November 2000.

29. The effect of age on the extension of the breeding season in the female mouflon

(Ovis gmelini musimon). International Congress on Mouflons. Sopron, Hungary. 27-29 October 2000

30. Estrus synchronization and artificial insemination in sheep. Symposium on the

development of sheep production systems in hot arid zones with special reference to Kuwait. Kuwait, 20-22 April 1998

31. Superovulation and embryo transfer in sheep. Symposium on the development of

sheep production systems in hot arid zones with special reference to Kuwait. Kuwait, 20-22 April 1998

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Participation in international scientific meetings. Short communications 1. Antimullerian hormone (AMH) as an age-related predictor of the antral follicle count

(AFC) and the response to exogenous FSH ovarian reserve test (EFORT) in sheep. 17th International Congress on Animal Reproduction (ICAR). Vancouver (Canada), 29 July - 2 August 2012.

2. Short- or long-term hypobaric hypoxia decreases corpus luteum size and insulin-like

growth factors in cycling sheep. 17th International Congress on Animal Reproduction (ICAR). Vancouver (Canada), 29 July - 2 August 2012.

3. Programming of hypothalamic gene expression in obese swine after nutritional

restriction in mid-late gestation. Prenatal Programming and Toxicity International Conference (PPTOX III). Paris (France), 14-16 May 2012.

4. Gender-specific early-postnatal catch-up growth after fetal growth retardation by

maternal food restriction in swine with obesity/leptin resistance. Prenatal Programming and Toxicity International Conference (PPTOX III). Paris (France), 14-16 May 2012.

5. Short-term undernutrition affects female fertility and embryo/foetal development in

obese pigs. 4th COST Action FA0702 GEMINI General Conference. Maternal Interactions with Gametes and Embryos. Gijon (Spain). 30 September – 2 October 2011.

6. Prostaglandin synchronization is associated to silent estrus in ewes reared at high

altitude. 2011 Annual Meeting of the European Federation of Animal Science. Stavanger (Norway). 29 August-2 September 2011.

7. Phenotypic characterization by high-resolution 3D-mri evidences differential effects of

embryo genotype on IUGR in Nos3-deficient mice. 3rd COST Action FA0702 GEMINI General Conference. Maternal Interactions with Gametes and Embryos. Soustons (France), 1-2 October 2010

8. Fat content in the diet affects early-embryo weight in sows with gene polymorphisms

for leptin and melanocortin receptors. 3rd COST Action FA0702 GEMINI General Conference. Maternal Interactions with Gametes and Embryos. Soustons (France), 1-2 October 2010.

9. Role of STAT3 pathway in the leptin-induced signaling during oocyte in vitro

maturation and steroidogenic response in rabbit model. 2010 Annual Meeting of the International Embryo Transfer Society. Córdoba (Argentina), 9-12 January 2010

10. Deleterious effects of progestagen treatment on functionality of preovulatory follicles

in sheep. 2010 Annual Meeting of the International Embryo Transfer Society. Córdoba (Argentina), 9-12 January 2010.

11. Incidence of embryo mortality and intrauterine growth retardation in a swine model

with obesity and leptin resistance (Iberian pig). 2nd Cost Action FA0702 Gemini General Conference. Maternal Interactions with Gametes and Embryos Alghero (Sardinia, Italy), 1-3 October 2009.

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12. Effects of leptin on maternal ovarian function and oocyte developmental competence in a mouse model of obesity/type 2 diabetes. 2nd Cost Action FA0702 Gemini General Conference. Maternal Interactions with Gametes and Embryos Alghero (Sardinia, Italy), 1-3 October 2009.

13. Kinetics of intramuscular di(2-ethylhexyl) phthalate in female sheep: a model to

research in female reproductive toxicology. 11th European Congress on Veterinary Pharmacology and Toxicology. Leipzig (Germany), 12 - 16 July 2009.

14. Influence of leptin on steroidogenic secretion and nuclear in vitro maturation via

MAPK pathway in the rabbit oocyte model. 2009. Annual Meeting of the Society for Reproduction and Fertility. Oxford (United Kingdom), 12-14 July 2009.

15. Relative roles of maternal and embryo genotypes in pregnancy outputs and postnatal

development of Nos3-knockout mice. Cost Action – Gemini "Maternal Interaction with Gametes and Embryos". Lanks (Poland), 7-8 May 2009.

16. Morphological and functional features of corpora lutea induced by administration of

cloprostenol in goats. II International Symposium on Animal Biology of Reproduction (ISABR 2008. Sao Paulo (Brazil), 19-22 November 2008.

17. Effect of Ageing on Follicle and Corpora Lutea Dynamics in Sheep. 12th ESDAR

Annual Conference. Utrecht (Holland), 20-23 November 2008. 18. Improvement of synchronization of preovulatory LH surge and ovulation in tropical

sheep treated with cloprostenol by the combination with male effect. 12th ESDAR Annual Conference. Utrecht (Holland), 20-23 November 2008.

19. Ultrasound biomicroscopy (UBM) for translational studies of feto-placental

development in non-anesthetized living mouse models of preeclampsia and intrauterine growth restriction. 2008 World Molecular Imaging Congress. Nice (France), 10-13 September 2008.

20. Ultrasound biomicroscopy imaging of ovarian features in animal models. 2008 World

Molecular Imaging Congress. Nice (France), 10-13 September 2008. 21. Effect of administration of L-glutamate on the onset of puberty in goats I: plasma

levels of triiodothyronine. IX International Conference on Goats. Sustainable goat production: Challenges and opportunities of small and large enterprises. Queretaro (México), 31 August-5 September 2008.

22. Effect of administration of L-glutamate on the onset of puberty in goats II: plasma

levels of triiodothyronine. IX International Conference on Goats. Sustainable goat production: Challenges and opportunities of small and large enterprises. Queretaro (México), 31 August-5 September 2008.

23. Sincronization of oestrus and ovulation in mice by administration of progesterone and

prostaglandin analogues. 16th International Congress on Animal Reproduction. Budapest (Hungary) 12-17 July 2008.

24. Synchronization of oestrus and ovulation in non-seasonal West African ewes treated

with cloprostenol during early stages of luteal development. 16th International Congress on Animal Reproduction. Budapest ((Hungary) 12-17 July 2008.

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25. Synchronization with progestagen affects LH receptor and function of corpus luteum in sheep. 16th International Congress on Animal Reproduction. Budapest (Hungary) 12-17 July 2008.

26. Expression of VEGF in Corpora Lutea of progestagen synchronized-sheep. 16th

International Congress on Animal Reproduction. Budapest (Hungary) 12-17 July 2008.

27. Development and function of corpus luteum in progestagen-synchronized sheep. 11th

ESDAR Annual Conference. Celle (Germany), 19-23 September 2007. 28. Expression of oestrogen receptor-α (ER-α) in sheep is affected by follicular size and

stage of the oestrous cycle. 11th ESDAR Annual Conference. Celle (Germany), 19-23 September 2007.

29. Efficacy of Low Doses of Cloprostenol for Synchronisation of Oestrous and Ovulation

in West African Ewes (Venezuela). 11th ESDAR Annual Conference. Celle (Germany) 19-23 September 2007.

30. Effectiveness of Low Doses of Cloprostenol for Estrus Induction in West African Ewes

(Venezuela). 10th ESDAR Annual Conference. Portoroz-Lipica (Slovenia), 7-9 September 2006.

31. Follicular development in response to exogenous FSH is affected within breeding

season. 7th International Ruminant Reproduction Symposium. Wellington (New Zealand), 13-17 August 2006.

32. Functionality of largest follicles is affected in gonadotrophin-stimulated cycles. 8th

International Congress on Endocrinology. Glasgow (United Kingdom), 1-5 April 2006. 33. Waves of follicle development during estrous cycle in young West African ewes in

Venezuela. XIX Reunión de la Asociación Latinoamericana de Producción Animal. Tampico (México), 26-28 October 2005.

34. Follicular development in response to a superovulatory treatment in sheep is delayed

by the presence of a dominant follicle. 21th Scientific Meeting, European Embryo Transfer Association. Kesthelzy (Hungary), 9-10 September 2005.

35. Reliability of plasma inhibin A measurement for surveillance of ovarian stimulation in

a sheep model. 2005 European Congress of Endocrinology. Goteborg (Sweden), 3-7 September 2005.

36. Effect of follicular status on sheep embryo yields is mediated by changes in the

preovulatory LH surge. 20th Endocrine Society of Australia (ESA) & Society for Reproductive Biology (SRB) Annual Scientific Meeting Perth (Australia), 4-7 September 2005.

37. Evidence for follicular co-dominance effects in superovulated sheep. 9th Annual

Meeting of the European Society of Domestic Animal Reproduction. Murcia (Spain), 1-3 September 2005.

38. Effects of progestagen on follicular growth and oocyte developmental competence in

FSH-treated sheep. 9th Annual Meeting of the European Society of Domestic Animal Reproduction. Murcia (Spain), 1-3 September 2005.

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39. Influence of the milk productive state on the response to a MOET protocol in Manchego ovine of milky aptitude. 9th Annual Meeting of the European Society of Domestic Animal Reproduction. Murcia (Spain), 1-3 September 2005.

40. Accuracy of ultrasonographic evaluation of presence and size of corpus luteum in

monovular hair sheep9th Annual Meeting of the European Society of Domestic Animal Reproduction. Murcia (Spain), 1-3 September 2005.

41. Timing of periovulatory events after a multiple-dose FSH treatment in sheep. Biennial

Joint Meeting of the UK Fertility Societies. Warwick, (United Kingdom), 3-6 April 2005. 42. Status of follicular population at starting a superovulatory treatment affects

occurrence of periovulatory events in sheep. Biennial Joint Meeting of the UK Fertility Societies. Warwick, (United Kingdom), 3-6 April 2005.

43. Fertilization in superovulated sheep is disturbed by oestrogenic anovulatory follicles.

24th Joint Meeting of the British Endocrinology Societies. Harrogate, (United Kingdom), 4-6 April 2005.

44. Ovarian response and developmental competence of oocytes collected by OPU in

sheep treated with GnRH-antagonist. International Embryo Transfer Society Meeting 2005. Copenhagen (Denmark), 9-11 January 2005

45. Cryoresistance of ovine embryos increases along cleavage stage.8th Annual

Conference. European Society for Domestic Animal Reproduction. Varsow (Poland), 23-25 September 2004

46. Seasonal changes in testicular activity, plasma prolactin, and melatonin

concentrations in mouflon (Ovis gmelini musimon) and Spanish merino (Ovis aries) rams. 8th Annual Conference. European Society for Domestic Animal Reproduction. Varsow (Poland), 23-25 September 2004

47. Inhibin A concentrations in sheep treated with growth hormone and GnRH antagonist.

Reproduction in Small Ruminants. Colonia (Uruguay), 5-6 August 2004 48. The effect of a single short-acting dose of GnRH antagonist on pituitary function in

sheep. Reproduction in Small Ruminants. Colonia (Uruguay), 5-6 August 2004 49. In vitro culture affects cryotolerance of ovine blastocysts irrespective of their origin.

Reproduction in Small Ruminants. Colonia (Uruguay), 5-6 August 2004 50. The presence of large follicles affects ovum pick-up efficiency in unstimulated sheep.

Reproduction in Small Ruminants. Colonia (Uruguay), 5-6 August 2004 51. The use of urinary and recombinant human FSH preparations to induce

superovulation in sheep and the effect on FSH and LH concentrations.British Society for Animal Science. York (United Kingdom), 5-7 April 2004.

52. Effects of growth hormone and GnRH antagonists on follicular and oocyte

development in sheep. International Embryo Transfer Society Meeting 2004. Portland, Oregon (U.S.A.), 10-15 January 2004.

53. Endocrine and ovarian function after GnRH antagonist treatments in goats.

International Embryo Transfer Society Meeting 2004. Portland, Oregon (U.S.A.), 10-15 January 2004.

26

54. Follicular growth in sheep superovulated with FSH after pre-treatment with GnRH

antagonists. International Embryo Transfer Society Meeting 2004. Portland, Oregon (U.S.A.), 10-15 January 2004.

55. Correlation with annual changes in the rate of horn growth of prolactin secretion and

testicular size in captive adult mouflon rams (Ovis gmelini musimon). XXVIth International Congress of the International Union of Game Biologist. Braga (Portugal). 1-6 September 2003.

56. Modification of follicular status in sheep treated with single short-acting doses of

GnRH antagonist. Joint Summer Meeting 2003. Society for the Study of Fertility. Aberdeen (United Kingdom), 13-17 July 2003.

57. The effect of a single long-acting injection of GnRH antagonist on ovarian follicular

development in sheep. Joint Summer Meeting 2003. Society for the Study of Fertility. Aberdeen (United Kingdom), 13-17 July 2003.

58. The effect of daily injections of GnRH antagonists on pituitary function in goats. Joint

Summer Meeting 2003. Society for the Study of Fertility. Aberdeen (United Kingdom), 13-17 Julio 2003.

59. Prolactin receptors in sheep ovary: regulated expression of the long but not the short

prolactin receptor isoform throughout the estrous cycle. International Embryo Transfer Society Meeting 2003. Auckland (New Zealand), 13-16 January 2003.

60. Testicular diameter and secretory patterns in melatonin and prolactin related to

photoperiod in Mouflon rams (Ovis gmelini musimon). IV International Mouflon Symposium. Nuoro (Italy), 10-12 October 2002.

61. Effects of melatonin implants on reproductive seasonality of Mouflon ewes (Ovis

gmelini musimon). IV International Mouflon Symposium. Nuoro (Italy), 10-12 October 2002

62. Influence of dominant follicles on maturation potential of oocytes from superovulated

ewes.6th Annual Conference. European Society for Domestic Animal Reproduction. Parma (Italy), 12-14 September 2002

63. Influence of physical ablation of dominant follicles on sheep superovulatory yields. 6th

Annual Conference. European Society for Domestic Animal Reproduction. Parma (Italy), 12-14 September 2002

64. Cryosurvival of sheep early embryos is influenced by intrinsic sensitivity to freezing.

18th Scientific Meeting, European Embryo Transfer Association. Kerkrade (Holland), 6-7 September 2002

65. Influence of ovarian status on number and viability of sheep early embryos. 18th

Scientific Meeting, European Embryo Transfer Association. Kerkrade (Holland), 6-7 September 2002

66. Differential reproductive response in Mouflon (Ovis gmelini musimon) and Manchega

(Ovis aries) ewes to artificial photoperiods. 5th International Congress of Neuroendocrinology. Bristol (United Kingdom), 31 August-4 September 2002.

27

67. Modification of follicular status in goats treated with GnRH antagonist (Teverelix INN). Sixth International Symposium on Reproduction in Domestic Ruminants. Crieff (United Kingdom), 14-17 August 2002

68. Extension of the breeding season and seasonal changes in plasma prolactin

concentrations in Spanish Ibex (Capra pyrenaica hispanica). Sixth International Symposium on Reproduction in Domestic Ruminants. Crieff (United Kingdom), 14-17 August 2002

69. Measurement of inhibin A and follicular status reflects embryo output in superovulated

goats. Joint Summer Meeting 2002. Society for Reproduction and Fertility. Leeds (United Kingdom), 7-10 July 2002.

70. Effects of urinary and recombinant human FSH preparations on superovulation in

sheep. Joint Summer Meeting 2002. Society for Reproduction and Fertility. Leeds (United Kingdom), 7-10 July 2002

71. Effect of ovarian status on maturation potential of sheep oocytes. Joint Summer

Meeting 2002. Society for Reproduction and Fertility. Leeds (United Kingdom), 7-10 July 2002

72. Localization of long and short prolactin receptors in the sheep ovary throughout the

estrous cycle. International Embryo Transfer Society Meeting 2002. Iguaçu (Brazil), 13-16 January 2002.

73. Plasma progesterone concentrations and estimation of fetal development in Mouflon

sheep (Ovis gmelini musimon) by transrectal ultrasonographic measurements. XXV Congress of the International Union of Game Biologists. Lemesos (Cyprus) 3-7 September 2001

74. Relationship between periovulatory and preimplantational estradiol levels and

superovulatory yields in sheep. International Embryo Transfer Society Meeting 2001. Omaha, Nebraska (U.S.A.). 13-16 January 2001.

75. Influence of season on inhibitory effects of dominant follicles in superovulated ewes.

Joint Winter Meeting 2000. Society for the Study of Fertility. Utrecht (Holland), 15-16 December 2000.

76. Presence of a corpus luteum at progestagen insertion positively affects embryo yields

from superovulated ewes. 4th Annual Conference of the European Society for Domestic Animal Reproduction. Prague, Czech Republic. 23-25 November 2000.

77. Ovarian follicular development during the follicular phase of the estrous cycle in

Mouflon. International Congress on Mouflons. Sopron, Hungary. 27-29 October 2000. 78. Effects of follicular population on endocrine response and ovarian response in

superovulated ewes. 16th Scientific Meeting, European Embryo Transfer Association. Santander (Spain), 8-9 September 2000.

79. Systemic and intraovarian effects of persistent large follicles on ovine follicular

growth. Joint Summer Meeting 2000. Society for the Study of Fertility. Edinburgh (United Kingdom), 31 July-2 August 2000.

28

80. Evidence of an endogenous circannual reproductive rhythm in female Mouflons (Ovis gmelini musimon) reared under a constant long-day photoperiod. ICAR 2000 Satellite Symposium on Reproduction in Small Ruminants. Sandnes (Norway), 30 June-1 July 2000.

81. Influence of plasma progesterone levels on number of transferable embryos obtained

from superovulated ewes. ICAR 2000 Satellite Symposium on Reproduction in Small Ruminants. Sandnes (Norway), 30 June-1 July 2000.

82. Ultrasonographic assessment of the seasonal changes in accessory glands and

testes in Mouflon rams (Ovis gmelini musimon). ICAR 2000 Satellite Symposium on Reproduction in Small Ruminants. Sandnes (Norway), 30 June-1 July 2000.

83. Embryo viability decreases during early development into genital tract of

superovulated ewes. International Embryo Transfer Society Meeting 2000. Utrecht (Holland) 7-9 January 2000.

84. Measurement of inhibin A predicts the superovulatory response to exogenous FSH in

sheep. Joint Winter Meeting 1999. Society for the Study of Fertility. Warwick (United Kingdom), December 1999

85. Seasonal changes in plasma melatonin concentrations in the mouflon (Ovis gmelini

musimon). VIII Meeting of the European Pineal Society. Amiens (France), 3-7 Julio 1999.

86. Gonadotrophin secretion and follicular dynamics after bromocriptine administration

during the follicular phase of the sheep estrous cycle. Joint Winter Meeting 1997. Society for the Study of Fertility. London (United Kingdom), December 1997.

87. Daily rhyhms of prolactinemia during different photoperiods in the mouflon (Ovis

ammon musimon). Joint Summer Meeting 1997. Society for the Study of Fertility. York (United Kingdom), 17-19 July 1997

88. Effect of follicular status prior to superovulatory treatments on the number and

viability of ovine embryos. I Congresso Iberico de Reproduçao Animal. Estoril (Portugal), 3-6 July 1997.

89. Effects of follicular development and superovulatory protocols on ovulation rate in

ewes I Congresso Iberico de Reproduçao Animal. Estoril (Portugal), 3-6 July 1997. 90. Variations in ovine follicular development during superovulatory treatment with

different FSH preparations. 12th Scientific Meeting of the European Embryo Transfer Association. Lyon (France), 13-14 September 1996.

91. Effect of follicular population on ovulatory response after a single injection of FSH-P

in ewes. 13th International Congress on Animal Reproduction. Sydney (Australia), 30 June- 4 July 1996.

92. Influence of the profiles of melatonin on the circannual rhythm of reproduction in

Mouflon (Ovis ammon musimon). 13th International Congress on Animal Reproduction. Sydney (Australia), 30 June- 4 July 1996.

93. Effect of follicular status prior to administration of exogenous gonadotropin on the

ovulation rate in ewes. International Embryo Transfer Society Meeting 1996. Salt Lake City, Utah (U.S.A.), 7-9 January 1996.

29

94. Ovarian follicular development during the follicular phase of oestrous cycle in

Manchega ewes with different ovulation rate. International Embryo Transfer Society Meeting 1996. Salt Lake City, Utah (U.S.A.), 7-9 January 1996.

95. Seasonal breeding activity and anoestrous postpartum in the mouflon (Ovis ammonn

musimon). Joint Winter Meeting 1995. Society for the Study of Fertility. Oxford, (United Kingdom), 17-19 December 1995

96. Influence of follicular population on ovulation rate in ewes after a single

superovulatory injection of FSH in propylene glycol. Joint Winter Meeting 1995. Society for the Study of Fertility. Oxford, (United Kingdom), 17-19 December 1995

97. Ultrasonic monitoring of ovarian follicular development in ewes during administration

of homologous FSH. International Symposium on Life Cycle of the Ovarian Follicle. Fort Lauderdale, Florida (U.S.A.), 2-5 November 1995.

Reviewer for competitive funding bodies 1. AGAE (Agencia Andaluza de Evaluación de la Calidad y Acreditación Universitaria,

Spain).

2. AgreenSkills (European Comisión).

3. ANEP (Agencia Nacional de Evaluación y Prospectiva, Spain)

4. ANPCyT-FONCyT (Agencia Nacional de Promoción Científica y Tecnológica, Argentina).

5. BBSRC (Biomedicine and Biological Science Research Committee, United Kingdom)

6. CSIC (Comisión Sectorial de Investigación Científica, Uruguay)

7. HMRF (Health and Medical Research Fund, Hong Kong).

8. InnoCash (Fundacion Española de Ciencia y Tecnología, Spain) 9. (FCT (Fundaçao para a Ciencia e a Tecnología, Portugal)

10. NRF (Nacional Research Foundation, South Africa) 11. The Wellcome Trust (United Kingdom).

Editor in Scientific Journals 1. PLOS ONE

2. Asian Pacific Journal of Reproduction

3. Dataset Papers in Biology

4. ISRN Anatomy

5. Physiology Journal

30

Reviewer in Scientific Journals

1. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology 2. Animal: An International Journal of Animal Bioscience 3. Animal Production Science 4. Animal Reproduction 5. Animal Reproduction Science 6. Archivos de Zootecnia 7. Biological Trace Element Research 8. Biology of Reproduction 9. BMC Veterinary Research 10. Canadian Journal of Veterinary Research 11. Ciencias Veterinarias FCV-LUZ 12. Clinical Interventions in Aging 13. Comparative Medicine 14. Diabetes 15. Domestic Animal Endocrinology

16. Endocrine, Metabolic & Immune Disorders - Drug Targets 17. Environmental Toxicology and Pharmacology 18. Fertility and Sterility 19. Free Radical Biology & Medicine

20. GENE 21. General and Comparative Endocrinology 22. Imaging in Medicine 23. International Journal of Developmental Neuroscience 24. International Journal of Experimental Pathology 25. Iranian Journal of Veterinary Research 26. ISRN Anatomy

31

27. Italian Journal of Animal Science 28. Journal of Animal Physiology and Animal Nutrition 29. Journal of Applied Animal Research 30. Journal of Assisted Reproduction and Genetics 31. Journal of Developmental Origins of Health and Disease 32. Journal of Endocrinology 33. Journal of Sexual Medicine 34. Journal of Veterinary Behavior: Clinical Applications and Research 35. Journal of Veterinary Science 36. Laboratory Animals 37. Livestock Science 38. Molecular and Cellular Endocrinology 39. PLoS ONE 40. Reproduction 41. Reproduction in Domestic Animals 42. Reproduction, Fertility and Development 43. Reproduction, Nutrition, Development 44. Reproductive Biology 45. Reproductive Biology and Endocrinology 46. Research in Veterinary Science 47. Small Ruminant Research 48. Spanish Journal of Agricultural Research 49. Theriogenology 50. The Veterinary Journal 51. Translational Research 52. Ultrasound in Medicine and Biology 53. Veterinarni medicina. International journal for veterinary sciences and biomedicine

32

54. Veterinary Medicine International 55. Zygote

Teaching experience. Graduate.

1. 2004-09. Assistant. Chair of Pharmacology and Toxicology, Faculty of Veterinary, Universidad Complutense de Madrid (Spain).

2. 2007-2008. Visiting Professor. Faculty of Veterinary, Universita degli Studi di Sassari

(Italy). 3. 2004. Seminars on Sheep reproduction. Faculty of Veterinary, Universita degli Studi

di Sassari (Italy). 4. 1999-2001. Assistant. Unit of Reproduction and Obstetrics, Faculty of Veterinary,

Universidad Complutense de Madrid (Spain). 5. 1998-99. Seminars of the Unit of Reproduction and Obstetrics, Faculty of Veterinary,

Universidad Complutense de Madrid (Spain). 6. 1996-97. Assistant. Unit of Reproduction and Obstetrics, Faculty of Veterinary,

Universidad Complutense de Madrid (Spain). 7. 1996. Fall Reproductive Physiology Seminars. College of Agriculture and Forestry.

West Virginia University, Morgantown (U.S.A.). 8. 1992-93 Assistant. Unit of Reproduction and Obstetrics, Faculty of Veterinary,

Universidad Complutense de Madrid (Spain). 9. 1990-92. Assistant Scholar, Unit of Reproduction and Obstetrics, Faculty of

Veterinary, Universidad Complutense de Madrid (Spain).

Teaching experience. Post-graduate. 1. Course on Technology of Swine Reproduction and Management of the Animals.

CEU-Universidad Cardenal Herrera. Valencia (Spain). 8-11 October 2012. 2. Present and Future of Reproductive Techniques. Universidad de Salamanca.

Salamanca (Spain), 2-10 March 2012. 3. International Course of Animal Reproduction. Spanish Agency of International

Cooperation. Madrid (Spain). October of each year from 1992 to 2011.

4. Course of Sheep Reproduction. Escuela Agraria de Cogullada. Zaragoza (Spain). January-February 2007.

5. Second Course on Synchronization of Oestrus and Artificial Insemination in Small Ruminants. Universidad Católica de Córdoba. Córdoba (Argentina), 28-31 August 2006.

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6. First Course on Synchronization of Oestrus and Artificial Insemination in Small Ruminants. Universidad Católica de Córdoba. Córdoba (Argentina), 22-25 August 2006.

7. Course on Artificial Insemination by Laparoscopy in Goats. Instituto Nacional de Investigaciones Agrícolas. Ministerio de Ciencia y Tecnología. Barquisimeto (Venezuela), 13-14 March 2006.

8. Fourth Internacional Course on Fisiología de la Reproducción en Rumiantes. Colegio de Postgraduados. Montecillo (México), 24-26 August 2005.

9. Course of Sheep Reproduction and Artificial Insemination. ITACyL and OVIGEN. Zamora (Spain), 17-19 May 2005.

10. Third International Course on Physiology of Reproduction in Ruminants. Colegio de Postgraduados. Montecillo (México), 23-26 September 2003.

11. Course of Assisted Reproduction in Small Ruminants. Universidad Nacional Autónoma de México. México DF (México), 15-18 September 2003.

12. Course of Physiology and Technology of Reproduction in Small Ruminants. Universidad Católica de Córdoba. Córdoba (Argentina), 17-21 March 2003.

13. Seminars in Reproduction and Genetic Improvement in Small Ruminants. Universidad Católica de Córdoba. Córdoba (Argentina), 18 March 2003.

14. Postgraduate in Animal Reproduction and Technology of Artificial Insemination. Universidad Central de Venezuela. Maracay (Venezuela), 28 October-4 November 2002.

15. Course of Human Assisted Reproduction. Universidad de Salamanca. Salamanca (Spain), 1-5 July 2002.

16. Practical Course “Technology of sheep reproduction”. Sociedad Española de Ovinotecnia y Caprinotecnia. Zaragoza (Spain), 25-28 February 2002.

17. Techniques of Fecundation, Reproduction and Embryo Handling. Universidad de Salamanca. Salamanca (Spain), 4 September –6 October 2000.

18. Course de Specialization pour la Production et Pathologie de la Chevre Murciano-Granadina”. HELP Foundation and Spanish Agency of International Cooperation. Murcia (Spain), 12-19 June 2000.

19. First Course of Management, Production and Pathology in Sheep and Goats. Universidad Complutense de Madrid. Madrid (Spain), 7 February- 21 March 1997.

20. First Course of Reproductive Technologies in Small Ruminants. Universidad de Castilla-La Mancha. Albacete (Spain), 26-29 March 1996.

21. Second Course of Reproduction in meat sheep. Community of Extremadura. Badajoz (Spain), 19-29 October 1993.

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Teaching experience. Masters 1. Human Reproduction. Universidad Complutense de Madrid. Madrid (Spain). From

2009. 2. Alterations in Reproduction. Universidad Complutense de Madrid. Madrid (Spain).

From 2009. 3. Biology and Technology of Reproduction. Universidad de Oviedo. Oviedo (Spain).

From 2011.

4. Technology of Reproduction. Universidad de Salamanca. Salamanca (Spain). From 2011.

5. Biotechnology of Reproduction in Veterinary. Universidad Complutense de Madrid.

Madrid (Spain). From 2012.

Director of PhD Theses 1. Non-invasive imaging of the effects of prenatal programming on postnatal growth and

fat deposition in an obese swine model. Alicia Barbero Fernandez. Universidad Complutense de Madrid. PhD degree to be obtained at May 2015.

2. Characterization of process and freezing media for sperm cryopreservation in the

Iberian breed. Eduardo de Mercado de la Peña. Universidad Complutense de Madrid. PhD degree obtained at September 2011.

3. Morphological and functional identification of endogenous factors determining reproductive efficiency in Iberian swine females. Pedro Gonzalez Añover. Universidad Complutense de Madrid. PhD degree obtained at September 2009.

4. Translational study of the effects of the endocrine disruptor di(2-etilhexil) phthalate

(DEHP) on the reproductive function of a female sheep model. Maria Angeles Herreros Guerra. Universidad Complutense de Madrid. PhD degree obtained at September 2009.

5. Non-invasive evaluation of pregnancy and embryogenesis in the mouse by the use of ultrasonographic imaging techniques. María Pilar Pallares García. Universidad Complutense de Madrid. PhD degree obtained at June 2008.

6. Short protocol for oestrus synchronization by the use of cloprostenol and “male effect” in West African sheep under tropical conditions. Ignacio Contreras Solis. Universidad Complutense de Madrid. PhD degree obtained at July 2008.

7. Characterization of ovarian and pituitary functionality in response to progestagen administration for oestrus synchronization in sheep. Claudia Aida Letelier Vasquez. Universidad Austral de Chile. PhD degree obtained at August 2008.

8. Characterization of dominance effects in superovulated sheep. Almudena Veiga López. Universidad Complutense de Madrid. PhD degree obtained at December 2005.

9. Pharmacokinetic and pharmacodynamic study of GnRH antagonists in sheep. Cristina Lopez Alonso Universidad Complutense de Madrid. PhD degree obtained at December 2004.

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Director of Master of Science Theses Adaptation and application of superovulatory techniques for embryo production in Rubia

del Molar and Negra de Colmenar sheep. Imad Ammoun. Mediterranean Agronomic Institute of Zaragoza. Master degree obtained at September 2009.

Financed projects for teaching development 1. Design and kick-off of a web-page for dissemination and knowledge of Animal

Welfare. INIA 2010-2011. 2. Development, by Magnetic Resonance Imaging, of an interactive atlas of

embryogenesis in different laboratory and farm animal species. INIA. 2008-2009. 3. International network for strengthening of teaching and research activities in the

postgraduate program of URUZA-UACH. CONACYT-FOMIX-DURANGO-UACH-URUZA. 2008-2010.

4. Virtual seminars: Information on veterinary drugs generated by EMEA and AEMPS.

UCM. 2006.