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CURRICULUM VITAE

SANKAR MITRA, Ph.D.

Date of preparation: October 02, 2013

A. GENERAL INFORMATION

Office Address: Houston Methodist Research Institute6670 Bertner Ave. Houston TX 77030

Office Telephone: 713-441-7148

Office Fax:

Home Address: 4003 W. Pine Brook Way

Houston, TX 77059-3016

Home Telephone: 281-488-5384 Cell Phone: 832-298-7550

Beeper:

Email: [email protected]

Citizenship: USA

Optional Information: N/A

Birth Date:

Birthplace:

Marital status:

Spouse’s Name: Children’s names and ages:

Race/Ethnicity:

B. EDUCATIONAL BACKGROUND

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Degree Institution name, city and state Dates Attended Year Awarded

Ph.D. University of Wisconsin 1964Madison Sept, 1960-Feb, 1964Biochemistry

M.S. Calcutta University April, 1957-March, 1959 1959Calcutta, India Biochemistry

B.S. Calcutta University April, 1952-March, 1957 1957Calcutta, India Chemistry

C. PROFESSIONAL POSITIONS AND EMPLOYMENT

Post-doctoral training including residency/fellowship

Title Institution Name, city and state Dates

Senior Research Scientist Bose Institute, Calcutta 1966-197 /Associate Professor

Postdoctoral Fellow Stanford Univ Medical School 1964-1965 (Enzymatic synthesis of DNA)

Research Assistant Department of Biochemistry 1960-1964and University Fellow University of Wisconsin

Research Fellow Department of Biochemistry1959-1960

Calcutta University

Academic positions (teaching and research)


Professor Department of Biochemistry & Molecular Biology, 7/2007-8/2013

Radiation Oncology and, Sealy Center for Molecular Medicine

The University of Texas Medical BranchGalveston, Texas

Professor The University of Texas Medical Branch 5/1992-6/2007

Galveston, TexasDepartments of Human Biological Chemistry and Genetics and of Radiation Oncology

Senior Scientist Sealy Center for Molecular Science and 5/1992-6/2007

The University of Texas Medical BranchGalveston, Texas.

Professor (Adjunct) University of Tennessee (Microbiology) 9/1971-4/1992

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Microbiology

Associate Professor Bose Institute 3/1966-4/1971Calcutta Department

Research Associate Department of Biochemistry 1/1964-12/1965Stanford Medical SchoolPalo Alto, CA

Hospital positions (attending physician, if applicable)


Full Member The Houston Methodist Research Institute 10/2012-present

Houston, TX

Employment (other than positions listed above)

Title Institution Name, city and state DatesN/A

D. LICENSURE, BOARD CERTIFICATION, MALPRACTICE (if applicable)Licensure

State Number Date of Issue Date of last registration N/A

DEA Number: N/A

Board Certification

Name of specialty Board Certificate Number Date of CertificationN/A

Malpractice Insurance

Do you have Malpractice Insurance? N/A

Name of Provider:

Premiums paid by:

E. PROFESSIONAL MEMBERSHIPS (medical and scientific societies)

Member/officer Name of Organization Dates held

Elected Fellow American Association for Advancement of Science 1985-present

Member Radiation Research Society 1991-1999

Member American Association for Cancer Research 1995-Present

Member American Society of Biochemistry & Mol Biol 1996- present

Member American Society for Microbiology 1998-2002

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Member Environmental Mutagenesis Genomic Society 1999-2003, 2012-present F. HONORS AND AWARDS

Name of award Date awarded

Panama National Plaque of honor 2011 Mark Brother’s of South Bend Indiana Lectureship Award, Indiana University 2009

Distinguished Faculty Research Award, Graduate School of Biomedical Sciences, UTMB 2003

Traveling Lecturer, Ministry of Education, (JSPS) Japan, 1999, 2008

Japan Society for the Promotion of Science Senior Scientist Award and Visiting Professor, Kyoto University 1991

Technical Achievement Awards, Martin Marietta Energy Systems, Inc. 1985, 1989

Tokten Consultant, United Nations Development Program (India) 1986-87, 1991-92

Guha Memorial Lecturer, Calcutta University (1983

NSF US/India Exchange of Scientists Award 1982

G. INSTITUTIONAL/HOSPITAL AFFILIATION

Primary Hospital Affiliation:

Other Hospital Affiliations:

Other Institutional Affiliations: Houston Methodist Research Institute

H. EMPLOYMENT STATUS

Name of Employer(s): Houston Methodist Research Institute Employment Status: Full-time salaried at Cornell-affiliated hospital

I. CURRENT AND PAST INSTITUTIONAL RESPONSIBILITIES AND PERCENT EFFORT

Teaching (list courses and your role) Dates

School of Medicine (SOM):

Lecture on Principles of Carcinogenesis to 1st year M.D./Ph.D. students 1999-2002 (1 1-hr lecture per year)

Facilitator for Pathobiology Course, MD/PhD Program (4-hr /year Spring 1999-2013

Prematriculation Lecture on Neoplasia to first year medical students (1-hr 2003-2008

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MCT course (1st yr class), 4 1-hr lectures/year on DNA transactions 2010- 2013

Graduate School (GSBS):Molecular and Cell Biology and Genetics Course ( 5 1-hr lectures/yr on DNA replication, repair and mutagenesis 1992 – 2013Biochemistry Course 1996-97Coordinator of Topics Course, "DNA Repair and Mutagenesis” (8 wk 2 credit course

Summer 2000, 2001, 2003Coordinator of a Special Topics Course, “DNA Protein Interaction” 1998, 2000, 2003Biochemistry (12 wk 3 credit course Fall 2003, 2004

Molecular Biology and Genetics Course Spring 2004-PresentTeaching Basic Radiobiology to Residents of Radiation Oncology, TMHRI 2012-2013(14 lectures)

Students/Mentees/Advisees/Trainees: 1992-present

Currently supervises a research group of 2 postdoctoral associates, 1 Ph.D. student (Arijit Dutta, 1st year) and 1 Research Associate and serves as a mentor to Drs. T. K. Hazra, Associate Professor Department of Internal Medicine) , and Kishor K. Bhakat and Bartosz Szczesny, Assistant Professors in the Department of Biochemistry and Molecular Biology (Non-tenure track), Dr. Muralidhar L. Hegde, Assistant Professor (Non-tenure track) Department of Neurology and Scientist, Department of Biochemistry and Molecular Biology, Dr. Partha Sarkar (Department of Neurology). Supervised research efforts of 10 graduate and 10 undergraduate students and about 25 postdoctoral fellows in the past.

Master’s students: 1996-PresentMember Thesis Committees of 8 Ph.D. Students

Undergraduate students: 1998, 2003, 2006-present

Supervisor of three high school students (summer program at UTMB)

GRADUATE STUDENTS (1992-2011):Dora Bocangel, (M.D. Anderson Cancer Center, Houston, TX) Lee Wiederhold received MD/PhD (Asst. Professor of Radiation Oncology, UTMB)Tapan Biswas received Ph.D. and are currently Visiting Scientists at UCSD,San Diego, CA and U. Miami, respectivelyCorey Theriot (Postdoctoral Fellow, NASA Johnson Space Center)Anne Tann (MD/PhD ), (Residency in Radiation Oncology at YTMB)Larry Bellot (Application Specialist, Nikon Inc)Jeff Hill (Univ of Miami)Elias Jackson( North Carolina A7T Univ, Raleigh)

PAST POSTDOCTORAL FELLOWS AND ACHIEVEMENTS AS A MENTOR: 1996-Present

Mentored six associates, Drs. R. Roy, I. Boldogh, T. Izumi, T. K. Hazra, K. K. Bhakat, Bartosz Szczesny and Aditi Das during 1995-Present, in their career development as independent research investigators.

Dr. Steve Boldogh (1995-2013) initially received an NCI R01 grant and subsequently several other grants. He is currently Professor of Microbiology & Immunology at UTMB.

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Dr. Rabindra Roy (1992-2000) moved to American Health Foundation, New York after receiving his NCI R01 grant while at UTMB. He has since received additional R01 and other grants and is currently Associate Professor (tenured) of Molecular Biology/Biochemistry and Lombardi Cancer Center at Georgetown University.

Dr. T.Izumi (1993-2003) moved to LSU Medical Center as Assistant Professor after receiving his first R01 grant from NIH while at UTMB which has just been renewed for another 5 years. He was Associate Professor at LSU Medical School and moved to University of Kentucky Cancer Center, Lexington as Associate Professor (tenured).

Dr. Tapas Hazra, (1994-2008) currently Professor in Department of Internal Medicine & Biochemistry and Molecular Biology recently received his second R01 grant, after his first R01 and an R21 grants.

Dr. Kishor Bhakat,(1997-2013) Assistant Professor of Biochemistry and Molecular Biology (non tenure track) at UTMB received an American Heart Association grant and been awarded his first R01 grant (2011-2016).He is currently tenure track Associate Professor at Univ Nebraska Cancer Center,Omaha.

Dr. Aditi Das (2002-2008) was at M.D. Anderson Cancer Center, Houston as an Instructor until she moved to NIH as Staff Scientist. She is Staff Scientist at NIH.

Dr. Hong Dou (2003-2005) is Assistant Professor at MD Anderson Cancer Center.

Dr. Bartosz Szczesny (2001-2013)is trying to compete for an NIH grant. He received a Pepper Center Young Investigator grant. He is a non tenure track Assistant Professor of Anesthesiology at UTMB.

Dr. Muralidhar L. Hegde, (2005-2013) non-tenure track Assistant Professor of Neurology has received research grants from American Parkinson’s Disease Association (2008-2010), Alzheimer’s Disease Association (2012-2014), UTMB NIEHS Center pilot grant (2012-2013); his research grant is under review in Muscular Dystrophy Association.

Dr. Corey Theriot, (2003-2009) after receiving Ph.D. in 2008, is currently a staff scientist at Johnson Space Center, Houston and Assistant Professor of Preventive Medicine,UTMB Galveston TX.

CHAIR/MEMBER OF PH.D. SUPERVISORY COMMITTEE FOR: 1995-2013

Corey TheriotLee WiederholdDora BocangelAnne TannTapan BiswasElias JacksonKash Choksi Debashish SahuAlex Ersadze

Clinical Care Not applicableDates

N/A

Administrative duties (including committees) Dates

UTMB

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UTMB APT Committee 2012-2016Founding member, Academy of Research Mentors 2012- 2013Steering Committee, Cancer Cell Biology Center 2007-2013Member, MD/Ph.D. Program Advisory Committee 2007–2013Internal Advisory Committee Member, NCI SPORE grant of UTMB 2006Space Review Task Force 2005-06Promotion and Tenure Committee 2003-04GSBS representative to Faculty Senate 2002-04Women’s Reproductive Health Research Center, Advisor 2001-2013Research Advisory Council 1998Children’s Hospital Research Core Advisory Committee 1997-2013tSealy Foundation Grant Reviewer 1996-2013Interviewer of Medical School Applicants, 8-12 per year 1995-2013

DEPARTMENTAL – (UTMB)

Member, Department APT Committee 2008-2011Member, Department Space Committee 2008-2013Ex-Offico and Chair, Academic Promotion & Tenure Committee 2007-2012Chair, Performance Evaluations Appeals Committee 2007-2009Vice Chair, Biochemistry and Molecular Biology 2007-2009Member, Internal Review Committee 2007-2008Chair, Recruitment Committee 2006-07Chair, Space Committee 2006-07Member, Committee to Revise Compensation Plan 2006-07Member, Appointment, Promotion & Tenure 2006-07Chair, Orientation Committee Graduate Program, Human Biol Chem & Genetics(HBC&G)

2004-05Member, Orientation Committee Graduate Program, HBC&G 2003-04Member, Examination Committee, Graduate Program, HBC&G 2002-03Member, Admissions Committee Graduate Program, HBC&G 2002-03Member, Orientation Committee Graduate Program, HBC&G 2002-03Chair, SCMS Science Forum 2002, 2003, 200Member, Credentials Committee 2000-02Chair, Credentials Committee 1999-00Member, Advisory/Orientation Committee, Graduate Program, HBC&G 1998-1999Member, Appeals Committee for Performance Evaluation 1997-98Member, Recruitment Committee 1997-98Chair, Student Advisory Committee 1997Member, Chairman's Advisory Committee 1996-97, 2004-05; 2006-10Member, Faculty Search Committee for Sealy Center for Structural Biology 1996-98

UTMB ActivitiesMember, Advisory Committee, Sealy Center for Molecular Medicine2011-2013NIEHS CENTER FOR MOLECULAR TOXICOLOGY, UTMB

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Member, Internal Advisory Committee 2008-2013Director, Research Core of DNA Repair and Mutagenesis 2004–2013Director, Research Core II, SubCore I, Oxidative Stress-Mediated Gene Regulation 1999-2004Director, Research Core I, Subcore IV, Oxidative Stress 1995-1999

Research Dates

1. Research Activities Genome damage due to gene activation (2012- )Oxidative demethylation of DNA and histones generates reactive oxygen species which oxidize bases and cause single-strand breaks. Their repair is a prerequisite for transcription activation. Specific Lys and Arg in histones are oxidatively demethylated by LSD1 and Jumonji demethylases which generate ROS. We have observed oxidized bases and strand breaks induced at enhancer sites containing methylated histones followed by their repair. The mechanism of repair is being examined.

2. Temporal regulation of radiation-induced clustered genome damage repair (2011-) Our recent studies demonstrate that repair of ionizing radiation-induced clustered genome damage is temporally coordinated with DSB repair occurring first via NHEJ followed by adjacent base damage repair initiated by DGs. NHEJ component Ku inhibits DGs and thus blocks BER before DSB repair which could otherwise generate more DSBs. We are testing the hypothesis that hnRNP-U acts as a ‘molecular switch’ via phosphorylation to allow NHEJBER transition. We also propose that the secondary DSBs generated during the BER of bi-stranded base damage are repaired by alternative end joining (Alt-EJ) which involves PARP-1 and BER proteins.

3. Back-up functions of DNA glycosylases (DGs) in oxidative base damage repair (2010- ) We have nearly solved the paradox of non-essentiality of individual DGs by investigating the roles of NEIL1/2.Fiber analysis of DNA replication in control and NEIL1 deficient human cells showed the requirement of NEIL1 in replication-coordinated repair as we had predicted. More significantly, we have now shown that NEIL2 can take over NEIL1’s repair function (with overlapping substrate range) in NEIL1 depleted cells.

4. Involvement of hnRNP proteins in repairing oxidative genome damage (2009- ) Based on our hypothesis that the BER complexes are specific, the presence of hnRNP proteins hnRNP-U, TDP-43 and Fus in immunoprecipitates (IPs) of DGs persuaded us to examine the role of these non-canonical proteins in BER. It is evident that these proteins significantly enhance the repair efficiency. The underlying mechanisms are being investigated.

5. Alkylating Drug Resistance of Tumor Cells (2003- 2011) We have acquired some significant insight into the mechanism of alkylating drug resistance of tumor cells. As is well known, acquired or intrinsic drug resistance is the primary cause of failure in chemotherapy. Overexpression of glycoprotein P (MDR1, MRP) has often been implicated in tumor resistance to many drugs. However, resistance to alkylating agents is not related to these transport proteins. We have carried out extensive studies using an isogenic pair of tumor cells which differ in their resistance to nitrogen mustard drugs. While some of the initial studies have just been published, other results, yet to be published, show how the tumor cells acquire resistance via complex cross talks between various regulatory and DNA repair proteins including p53, and how ROS chronically generated by alkylating agents, plays a critical role in drug-induced apoptosis. Our recent results indicate indirect role of APE1 as an activator of the MDR1 gene.

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6. Structure-Function Studies of Base Excision Repair Proteins (2002- ) Base excision repair (BER) is distinct from other repair pathways such as nucleotide excision and mismatch repair processes. Unlike the latter, no genetic disease has been linked to the deficiency of BER proteins. However, BER is extremely important because of its role in repairing endogenous DNA damage induced by oxidative stress, alkylation and other nonenzymatic reactions. Such lesions include modified bases as well as abasic (AP) sites and DNA strand breaks.

In a NIH-funded project, our main objectives are to elucidate the structure-function relation of DNA glycosylases and APE1, involved in the first two steps in the BER pathway. The approaches include site-directed and random mutagenesis of cloned cDNAs, overexpression, and physicochemical characterization of mutant proteins.

7. Discovery of a Distinct Family of Oxidized Base-Specific Mammalian Repair Enzymes (2002- )We discovered in 2002, subsequently confirmed by others, two oxidized base-specific DNA glycosylases in the human genome data base which we named NEIL1 and NEIL2. We also identified a third member of the family and named it NEIL3. We then cloned the genes, expressed the proteins and tested their activities. We could not find any activity in NEIL3. But NEIL1 and NEIL2, members of the E. coli Fpg/Nei family, are structurally distinct from the other two well characterized mammalian oxidized base-specific DNA glycosylases OGG1 and NTH1. These also have unusual properties in using single-stranded and bubble DNA substrates. Our current efforts are aimed at establishing that NEIL1 is involved in preferential repair of replicating DNA while NEIL2 repairs damage in transcribed strand during RNA synthesis. The latter studies are being pursued independently by my junior Faculty Associate Dr. Tapas Hazra who has received a 5-year NIH R01 grant for these studies.

8. In Vitro Base Excision Repair Studies (2001- ) Protein-protein interaction in DNA baseexcision repair in mammalian cells.Although BER involves fewer enzymes than other excision repair pathways, it is evident that the in vivo repair is much more efficient than observed in the in vitro studies. This suggests that enhanced efficiency of the multiple reaction process may be due to interaction among BER proteins. This is supported by our recent studies which show interaction between APE1 and flap endonuclease (FEN1) which acts on the DNA cleaved by APE1. We are exploring other interaction among the repair proteins by using a variety of techniques.

9. Discovery of a Second 8-oxoguanine Repair Enzyme (1999-2001)8-oxoguanine is arguably the most important ROS-induced mutagenic lesion and has been implicated in tumorigenesis and tumor progression. The ubiquitous enzyme, 8-oxoguanine-DNA glycosylase (OGG), is responsible for its repair via the DNA base excision repair pathway. OGG does not remove the oxidized base when paired with A, and 8-oxoG is mutagenic because of its pairing with A during DNA replication. The hypothesis of “GO system” was proposed to provide a rationale for this unusual substrate specificity. Our laboratory has recently identified a second OGG, OGG2, which is also ubiquitous. OGG2 does prefer 8-oxoguanine as the substrate when it is paired with G or A. This observation led us to propose a modified model of the GO system which we named, “Bipartite antimutagenic processing of 8-oxoguanine”. This model predicts that OGG2 removes 8-oxoguanine from the nascent DNA strand when it is incorporated opposite A from the nucleotide pool. Our recent unpublished results showing that OGG2 is strongly stimulated by the mismatch recognition protein MutS (in the E. coli system) support one prediction of the model that OGG2 utilizes the DNA mismatch repair pathway for identifying the nascent strand. Similar studies are currently being carried out with the human OGG2.

10. Regulation of DNA Repair Genes by Signal Transduction (1992-2006)

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Several DNA repair genes including O 6-methylguanine-DNA methyltransferase (MGMT) and the major AP-endonuclease (APE) have been found to be highly regulated in many nontumor and tumor cells. Elucidation of the molecular mechanisms of regulation is important from both basic and clinical perspectives.

a .Regulation of APE (1992- )Preliminary studies from our laboratory showed for the first time that the human AP-endonuclease (APE1) gene is transiently activated by subtoxic level of oxidative stress. Furthermore, the activation is associated with increased resistance only to genotoxic agents that induce reactive oxygen species. This phenomenon of "adaptive response" is distinct from other similar phenomena and may have far reaching implication because APE has other unrelated activities. It is a redox activator of several transcription factors including c-Fos, c-Jun and CREB. It is also a corepressor of parathyroid hormone and possibly several other Ca2-regulating genes due to binding to negative Ca2+ response elements (nCaRE). We have shown that APE may be autoregulated. Thus APE1 binds to nCaRE in its own promoter as a complex with another protein which we have recently identified to be hnRNP-L. More recently, we discovered acetylation of APE1 in vivo which modulates its transcriptional regulatory function and established that mutation in the acetyl acceptor lysine residues in APE1 induces cell lethality.

The major objectives of the NIH-funded projects are to elucidate the molecular mechanisms of APE1’s regulatory role in various pathologies including cancer and aging. In a complementary study funded by a National Institute of Aging Program Project, age-dependent regulation of APE gene and its consequences is being investigated.

b. Regulation of MGMT (1989-2006)It is long known that expression level of MGMT varies widely in tissue-specific fashion and that in some tumor and transformed cells MGMT, (named Mex) expression is undetectable while in many other metastatic tumors MGMT expression is very high. Because MGMT acts singly in removing the major precytotoxic lesion induced by chloroethyl-N-nitrosourea (CNU)-type drugs, e.g. carmustine, there is an excellent correlation between CNU resistance and overexpression of MGMT.

The objective of NIH-funded projects was to elucidate the mechanisms for overexpression as well as extinction of the MGMT gene in human tumor cells. Identification of specific signaling or trans-acting factors may help design drugs to target these in order to downregulate MGMT. Lowering MGMT will invariably result in increased therapeutic index of CNU.

Characterization of MGMT promoter led to identification of several putative cis-elements including glucocorticoid response elements (GRE), AP-1 and Sp1 which were found to be functional in vivo in several cell lines. This observation has profound clinical significance because glucocorticoids are often administered in conjunction with CNU drugs in chemotherapy for some tumors. More recent results indicate that the MGMT gene is activated by trichostatin, an inhibitor o histone deacetylase. The prediction from this study that chromatin remodeling involving the presence of hyperacetylated histone is required for MGMT activation has been confirmed in several ways.

Key Scientific Discoveries Made by the Mitra Lab: (1980-2013)Discovery of a novel role of NEIL1 in replicative DNA repair (2013) Discovery of the role of Fe/Cu as double-edged swords in inducing oxidative genome damage and inhibiting its repair, and its implication in neurodegenerative diseases.( 2010 ) Discovery of hnRNP family members’ role in DNA repair. (2009)

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Discovery of a mitochondrial 5 exonuclease (EXOG) responsible for long-path repair of oxidized AP-sites in DNA. (2008)

Discovery of preformed BER complexes with the glycosylase defining sub-pathways of BER via direct interaction with distal components of the repair pathway. (2007)Identification of mitochondrial APE as a N-terminal truncation product of APE1.( 2006)Establishing two distinct essential functions of APE1. Generation of conditional APE1 mutant mouse embryo fibroblast lines. (2005)

Discovery of a novel base excision repair pathway involving NEILs. (2004)

Discovery of covalent modifications (acetylation) of APE1, a key human repair enzyme and subsequent discovery of acetylaion of human DNA glycosylases, OGG1 and NEIL2 and its physiological impact.(2003-2005)

Discovery and naming of a new family of oxidized base-specific human repair enzymes NEIL1 and NEIL2. (2002)

Discovery of APE1 activation by oxidative stress. (1996)

Cloning and characterization of the human MGMT gene using phenotypic complementation in E. coli. Subsequent cloning of the human N-methylpurine- DNA glycosylase cDNA using the same strategy. ( 1990-1993)

Discovery of an unusual, suicide protein for repairing O6-methylguanine – named Ada and development of quantitative assay; naming of the generic protein as MGMT. (1980)

Current percent effort % Check if activity involves WCMC Students/researchers N/A

Teaching(MD and PhD students) 10 Clinical Care Administration 5 Research_________________ ____ 85_______

Total: 100%

J. RESEARCH SUPPORT (past and present)CURRENT

1R01 CA158910-01A1 (Mitra, PI,15% effort) 03/01/12 – 02/28/17 ($317,485/YR, administrative reduction from approved $385,000/YR) NIH/NCI “Repair Co-ordination of Radiation-Induced Clustered Damage in Mammalian Genomes” The aims of this project are: (1) To identify the requirements for repair of novel, linearized reporter-plasmids with dirty ends and clustered lesions using repair-deficient control and irradiated cells, and with nuclear/chromatin extracts from these cells, and assess relative contribution of Alt-EJ to overall repair. (2) To test the hypothesis that hnRNP-U (and Ku) act as molecular switch(es) via DNA-PK-mediated phosphorylation to co-ordinate NHEJ and BER-SSBR/Alt-EJ, by using cells and ICs containing altered levels of critical proteins (including PARP-1 and DNA polymerases u or λ) and identify phosphatase(s). (3) To test the hypothesis that NHEJ and BER-SSBR (Alt-EJ) proteins form dynamic complexes, by monitoring the kinetics of their formation, repair activity, nuclear co-localization, and role of covalent modifications during IR-induced enhanced complex formation.

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2P01 CA92584 (J. Tainer, PI; Mitra, Co-I: 5% effort) 09/01/11 – 08/31/16 ($63000/YR)NIH/NCI“Structural Cell Biology of DNA Repair Machines”The aims of this project are: (1) Interaction of APE1 with XRCC1, FEN1, PCNA, and HDAC1 (Collaboration with Tainer and Tomkinson); (2) Interaction with NEIL1 and PCNA (Collaboration with Matsumoto and Tainer); (3) Interaction of NEIL1 and NEIL2 with XRCC1/DNA ligase III (Collaboration with Tomkinson and Tainer), and (4) Interaction of NEIL1 and NEIL2 with XPG (collaboration with Cooper and Tainer).Overlap: None.

Melo International grant, PanamaGenome damage repair in brain disordersMitra, Co-PI (5% effort ; 2011-2013) Supports exchange student from Panama is conducting research in Mitra Lab

PENDING:

2R01 CA81063-12A1 (Mitra, PI; 15% effort) 6/01/14 – 5/31/19 NIH/NCI ( revised application to be submitted from TMHRI in 10-13)“Repair of Oxidized Bases in Mammalian Genomes”The aims of this project are: (1) NEIL1 forms distinct repair complexes in G1 vs. S-phase cells, and in response to oxidative stress; (2) NEIL1 preferentially repairs base damage in both template and nascent DNA during replication by interacting with the replicating complex; (3) damage in replicating plasmid is preferentially repaired in vivo relative to nonreplicating plasmid; and (4) NEIL1-induced cleavage at the damage site of the replicating DNA strand leads to double-strand breaks. Overlap: Competing renewal. 1R01GM105090-01A1 (Mitra, PI; 15% effort) to be funded 2/01/2014 – 1/31/2018 ($385,000/YR)NIH/NIGMS“Repair of Oxidative Genome Damage Associated with Gene Activation”The aims of this project are: (1) To test the hypothesis that enhancer-specific base damage and SSBs during gene activation and their repair are universal. (2) To test the hypothesis that efficient SSBR/BER linked to gene activation requires specific complexes whose formation is enhanced by the ligand. RECENTLY COMPLETED:John Sealy Memorial Bridging Grant (Mitra, PI 5% effort) 01/01/2013 – 12/31/2013 ($50,000) Salary support 0%

1U13CA13602001 (Mitra, PI,5% effort)NIH/NCIConference Grant for partial support of the “3rd US/EU Conference on Repair of Endogenous Genome Damage” to be held February 21-25, 2009 at Moody Gardens Hotel, Galveston, TX

2R01 CA81063-13 (Mitra, PI; 15% effort) 02/01/07 – 01/31/13 (with no cost extension)NIH/NCI“Repair of Oxidized Bases in Mammalian Genomes”The aims of this project are: (1) NEIL1 forms distinct repair complexes in G1 vs. S-phase cells, and in response to oxidative stress; (2) NEIL1 preferentially repairs base damage in both template and nascent DNA during replication by interacting with the replicating complex; (3) damage in replicating plasmid is preferentially repaired in vivo relative to nonreplicating plasmid; and (4) NEIL1-induced cleavage at the damage site of the replicating DNA strand leads to double-strand breaks.

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https://public.era.nih.gov/grantfolder/piAppDetails/genericStatus.do?encryptedParam=xayxPtEGwPE.dvWMAsVrQPdLc2VhmZKpTGspM5wnJXToffYKHvZv4tI.

Overlap: Competing renewal.

2 R01 CA53791-21S1 (Mitra, PI,15% effort) 07/01/06 – 5/31/13 (with no cost extension)NIH/NCI“Repair of Endogenous Damage in Mammalian Genomes” The aims of this project are: (1) generate transfectants of APE1 conditional MEF mutants for stable expression of APE1’s regulatory function in order to exclusively examine APE1’s repair activity for preventing apoptosis. (2) test the hypothesis that APS and OAS in the mt genome provide the initial ROS signal for apoptosis by measuring ROS and the kinetics of APS and OAS formation in the absence of APE1; (3) purify and clone RAAP in order to show that its inhibition prevents APE1’s cleavage and hence mt accumulation, leading to apoptosis, and finally (4) test the hypothesis that the nuclear APS and OAS activate both p53 and p73 for inducing apoptosis.

R01 ES/CA 08457-10 (Mitra, PI,20% effort) 05/01/06 – 04/30/11NIH/NIEHS “Regulatory Functions of APE1 – An Essential Repair Protein”

1PO1AG021830 NIH/NIA 09/15/04 – 07/31/10(J.Papaconstantinou, PI; Mitra, PL)“Oxidative Stress, Mitochondrial Dysfunction and Aging”Project 2 (PL, Mitra) “Age-Dependent Modulation of AP-Endonuclease Functions”

PAST:

NCI Division of Cancer Biology’s Activities to Promote Research Collaborations Coordinating Project Title: “Structural Cell Biology of XPG Interactions in DNA Repair” Coordinating Principal Investigator: Priscilla Cooper, Ph.D. 1998-2001

National Institutes of Health, National Center for Research Resources, DHHS 1 S10 RR12954, Shared Instrument Grant ($86,800). 1998-1999

NIA P01 Grant, titled, “Effects of Aging on Molecular Responses to Stress” (P.I. of Project 6). 1997- 2002

Supplemental equipment funds ($30,000) from NIEHS and NCI. 1997-1998

National Institute of Environmental Health Sciences, R01 ES/CA08457-06, “Regulation of Radiation/Oxidative Damage Repair in DNA”, (P.I.). 1996-2005

University of Texas Medical Branch Center on Aging, “Effect of Aging on Oxidative stress-Response in Mammals” (P.I.). 1996-97

National Institute of Environmental Health Sciences, R01 ES 07572-08, “Regulation of Alkylation Damage Repair Genes of Mammals” (P.I.). (Voluntary Termination). 1995-2000

John Sealy Memorial Endowment Fund for Biomedical Research, "Cloning of Alkylation Resistance-Conferring Genes from Human Breast Tumors" (P.I.). 1994-1995

National Cancer Institute, Grant RO1 CA 31721-22, titled "DNA repair and nitrosamine-induced carcinogenesis" (P.I.). (Voluntary Termination). 1982-97

K. EXTRAMURAL PROFESSIONAL RESPONSIBILITIES

EDITORIAL BOARD

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DNA Repair (2013-)Journal of Biological Chemistry (USA; 2007-2012)Molecules to Cells, (Korea) (2005-Present)Journal of Radiation Research (Japan) (2007-PresentAnticancer Research (Greece) (2002-Present)Mechanisms of Aging and Development (2011-Present)

JOURNAL REVIEWER 1985-Present

Anticancer ResearchCancer ResearchCarcinogenesisFree Radicals Biol. Med.Mutation Research, Journal of Biological ChemistryBiochemistryBiochemica Biophys Acta.; Antimicrobial Agents and ChemotherapyEnvironmental Health Perspectives; Molecular and Cellular BiologyMutagenesisNatureNucleic Acids Research Oncogene SciencePNAS, PLos OnePLos BiologyJ.Biol Chem. J. Mol Biol.Mol Cell BiolOncogene

GRANT REVIEWER - Reviewer of NSF and DOE grant applications.

Reviewer of CSR NIH MGB, MGC, CE ad hoc until 2009)

Convener, US/EU Repair of Endogenous Genome Damage Meeting, Galveston (2009) Ad Hoc Reviewer, CPA Study Section, CSR, USPHS (2003-Present)

NIA Site Visit Team, Washington State University (2002, 2003)

External Reviewer, Canadian National Cancer Institute Grants (2002)

NCI P01 Reviewer of Indiana University (2001)

Convener, BER 2000 Workshop, Galveston (2000)

Ad hoc Reviewer NCI Intramural Program at Frederick (1998, 2006)

Reviewer of NIEHS Center Grant Review Committee, Vanderbilt (1998, 2003)

Member of Site Visit Review Panel, National Institute of Environmental HealthSciences, (1996-Present)

Consultant, MBRS Program, Tuskegee University (1996-Present)

Member, Molecular and Cell Biology Study Section, Breast Cancer Research

14

Program, University of California (1995)

Reviewer, German/Israel Collaborative Research Program (1994-Present)

Moderator, NIH Workshop, "Molecular Studies Relevant to Radiation Oncology" (1994)

Consultant, National Cancer Institute (1992-Present)

Member, Radiation Study Section, Division of Research Grants, NIH (1992-1996)

Special member, Site Visit Panels for Program Projects and Cancer Centers and Consultant, National Cancer Institute (1990-Present)

NCI SEP for R13 grants (2010-)Lytmos Inc (2009-)INTERNATIONAL

Reviewer, UK Cancer Research 2012

Reviewer, Telethon Foundation Grant, Italy 2008

Ad hoc reviewer of grants submitted to Department of Biotechnology, India2007

Ad hoc reviewer of grants submitted to Cancer Research, U.K. 2009

External Reviewer, Canadian National Cancer Institute Grants 2002

Advisory Committee, International Institute for Anticancer Research, Greece 1997

Reviewer, German/Israel Collaborative Research Program 1994-

NATIONAL

Ad hoc member, Board of Scientific Counselors, NIEHS 2012

Ad hoc member of Board of Scientific Counselors at National Cancer Institute for reviewing intramural program 2012

Ad hoc member of Board of Scientific Counselors at National Institute on Aging for intramural program review 2012

Ad hoc reviewer, Bankhead Cooley Foundation grants (2011 Member, External Advisory Board of Markey Cancer Center, University of Kentucky, Lexington

2010-PresentMember, External Advisory Committees of NCI P01 grants, MD Anderson Cancer Center

2010-2014Ad hoc reviewer of grants submitted to Phillip Morris Research Foundation

2007Reviewer of CSR NIH MGB, MGC, CE (ad hoc 1-2 per year)

2004-2009Ad hoc Council Member, National Institute on Aging

2004Member, External Advisory Committees of NCI P01 grants, Emory University

2004-2007Ad Hoc Reviewer, CPA Study Section, CSR, USPHS

2003-PresentNIA Site Visit Team, Washington State University 2002, 2003NCI P01 Reviewer of Indiana University 2001

15

Convener, BER 2000 Workshop, Galveston 2000

Reviewer of NIEHS Center Grant Review Committee, Vanderbilt1998, 2003

Ad hoc Reviewer NCI Intramural Program at Frederick 1998, 2006

Consultant, MBRS Program, Tuskegee University 1996-PresentMember of Site Visit Review Panel, National Institute of Environmental Health Sciences

1996-Present Member, Molecular and Cell Biology Study Section, Breast Cancer Research Program, University of California 1995 Moderator, NIH Workshop, "Molecular Studies Relevant to Radiation Oncology"

1994 Member, Radiation Study Section, Division of Research Grants, NIH

1992-1996 Consultant, National Cancer Institute 1992-Present Consultant, National Cancer Institute 1992-PresentPresent Special member, Site Visit Panels for Program Projects and Cancer Centers and Consultant,

National Cancer Institute 1990-Present

Reviewer of NSF and DOE grant applications 1988-2000

OTHER

External Advisor, Program Project Grant, American Health Foundation2003

External Advisor, Program Project Grant, Indiana University School of Medicine 2003

Organization Committee, M.D. Anderson Basic Research Symposium 2002

Martin Marietta Energy Systems Awards Committee 1989-91

Division representative, Oak Ridge National Laboratory Seed Money Committee1984-86

L. BIBLIOGRAPHY

Peer Reviewed Articles

1. Hegde ML Hegde PM, Bellot LJ, Mandal SM, Hazra TK, Li GM, Boldogh I, Tomkinson AE and Mitra S. (2013) Prereplicative Repair of Oxidized Bases in Human Genome Is Mediated by NEIL1 DNA Glycosylase in Association with Replication Proteins. PNAS, 2013, 110(33):E3090-9 (Highlighted in FOX News and various media)

2. Szczesny B, Olah G, Walker DK, Volpi E, Rasmussen BB, Szabo C, Mitra S. Deficiency in repair of the mitochondrial genome sensitizes proliferating myoblasts to oxidative damage. PLoS One. 2013 8(9):e75201

3. Sengupta S, Mitra S and Bhakat KK (2013) Dual Regulatory Roles of Human AP-endonuclease (APE1/Ref-1) in CDKN1A/p21 Expression. PLoS One 8(7) e68467.

4. Hegde ML, Tsutakawa SE, Hegde PM, Holthauzen LM, Li J, Oezguen N, Hilser VJ, Tainer JA and Mitra S. (2013) The Disordered C-terminal Domain of Human DNA Glycosylase NEIL1

16

http://www.ncbi.nlm.nih.gov/pubmed/24066171


Contributes to Its Stability via Intramolecular Interactions. J Mol Biol. 425: 2359-2371. 2013 PMID:23542007

5. Tsutakawa SE, Shin DS, Mol CD, Izumi T, Arvai AS, Mantha AK, Szczesny B, Ivanov IN, Hosfield DJ, Maiti B, Pique ME, Frankel KA, Hitomi K. Cunningham RP, Mitra S, Tainer JA. (2013) Conserved Strucutral chemistry for Incision Acivity in Structurally Non-homologous Apurinic/Apyimidince Endonuclease APE1 and Endonuclease IV DNA repair enzymes. J Biol Chem 288(12):8445-55. PMID:233554.

6. Hegde ML, Banerjee S, Hegde PM, Bellot LJ, Hazra TK, Boldogh I, Mitra S. (2012) Enhancement of NEIL1 protein-initiated oxidized DNA base excision repair by heterogeneous nuclear ribonucleoprotein U (hnRNP-U) via direct interaction. J Biol Chem 287(51)34202-11. PMID:22902625.

7. Della-Maria J, Hegde ML, McNeill DR, Matsumoto Y, Tsai MS, Ellenberger T, Wilson DM, Mitra S, Tomkinson AE. (2012) The interaction between Polynucleotide Kinase Phosphatase and the DNA Repair Protein XRCC1 is Critical for Repair of DNA Alkylation Damage and Stable Association at DNA Damage Sites. J Biol Chem 287:39233-39244.

8. Hegde ML, Hegde PM, Dutta A, Boldogh I and Mitra S. (2012) Human DNA Glycosylase NEIL1’s interactions with downstream repair proteins is critical for efficient repair of oxidized genome damage and enhanced cell survival. Biomolecules. 2, 564-578

9. Hegde ML, Izumi T and Mitra S. (2012) Oxidized Base Damage and Single-Strand Break Repair in Mammalian Genomes: Role of Disordered Regions and Posttranslational Modifications in Early Enzymes. Progress in Molecular Biology and Translational Science 110:123-53.. PMID:22749145.

10. Boldogh I, Hajas G, Aguilera-Aguirre L, Hegde ML, Radak Z, Bacsi A, Sur S, Hazra TK, Mitra S. (2012) Activation of ras signaling pathway by 9-oxoguanine DNA glycosylase bound to its excision product, 8-oxoguanine. J Biol Chem 287(25):20769-73. PMID:2256894.

11. Mantha AK, Dhiman M. Taglialatela G, Perez-Polo RJ, Mitra S. (2012) Proteomic study of amyloid beta (25-35) peptide exposure to neuronal cells: Impact on APE1/Ref-1’s protein-protein interaction. J Neurosci Res. 90(6):1230-9. PMID:22488727.

12. Sengupta S, Chattopadhyay R, Mantha AK, Mitra S, Bhakat KK. (2012) Regulation of mouse-renin gene by apurinic/apyrididinic-endonuclease 1 (APE1/Ref-1) via recruitment of histone deacetylase 1 corepressor complex. J Hypertens (5):917-25. PMID:22441348.

13. Hegde ML, Mantha AK, Hazra TK, Bhakat KK, Mitra S, Szczesney B. (2012) Oxidative genome damage and its repair: implications to aging and neurodegenerative diseases. Mech Aging Develop. 133(4):157-68. Pmid:22313689.

14. Oezguen N, Mantha AK, Izumi T, Schein CH, Mitra S, Braun W. (2012) MD simulation and experimental evidence for Mg2+ binding at the B site in human AP endonuclease 1. Bioinformation 7(4):184-98. PMID:22102776.

15. Choi K, Chen J, Mitra S, Sarna SK. Impaired Integrity of DNA after Recovery from Inflammation Causes Persistent Dysfunction of Colonic Smooth Muscle. Gastroenterology. 2011:141 1293-1301. PMID: 21745450

16. Hegde ML, Hegde PM, Rao KS, Mitra S. Oxidative genome damage and its repair in neurodegenerative diseases: function of transition metals as a double-edged sword. J Alzheimers Dis. 2011;24 Suppl 2:183-98. PMID: 21441656

17








paperpal:23612479?host=www.ncbi.nlm.nih.gov&proxies=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

17. Tann AW, Boldogh I, Meiss G, Qian W, Van Houten B, Mitra S, Szczesny B. Apoptosis induced by persistent single-strand breaks in the mitochondrial genome: Critical role of EXOG (5' EXO/Endonuclease) in their repair. J Biol Chem. 2011:286 31975-83. PMID: 21768646

18. Zhang H, Xie C, Spencer HJ, Zuo C, Higuchi M, Ranganathan G, Kern PA, Chou MW, Huang Q, Szczesny B, Mitra S, Watson AJ, Margison GP, Fan CY. Obesity and hepatosteatosis in mice with enhanced oxidative DNA damage processing in mitochondria. Am J Pathol. 2011 Apr;178(4):1715-27. PMID: 21435453

19. Sengupta S, Mantha AK, Mitra S, Bhakat KK. Human AP endonuclease (APE1/Ref-1) and its acetylation regulate YB-1-p300 recruitment and RNA polymerase II loading in the drug-induced activation of multidrug resistance gene MDR1. Oncogene. 2011 30(4):482-93. PMID: 20856196

20. Hegde ML, Hazra TK and Mitra S. Functions of disordered regions in early base excision repair proteins. Cell Mol Life Sci 67:3573-87, 2010. PMID: 20714778

21. Hegde ML, Hegde PM, Holthauzin LM, Hazra TK, Rao KSJ, and Mitra S. Specific inhibition of NEIL-mediated repair of oxidized bases in genome by copper and iron: Potential etiological linkage to neurodegenerative disorders. J Biol Chem 285:28812-25, 2010. PMID: 20622253 *Highlighted in Spotlight section of Chemical Research Toxicology (Rouzer CA, Metals and DNA Repair, Chem. Res. Toxicol., 2010, 23 (10), pp 1517– 1518). Faculty1000 citation.

22. Banerjee D, Mandal SM, Das A, Hegde ML, Das S, Bhakat KK, Boldogh I, Sarkar PS, Mitra S, and Hazra TK. Preferential repair of oxidized base damage in the transcribed genes of mammalian cells. J Biol Chem, 2011;286(8):6006-16. PMID: 21169365

23. Szczesny B, Tann AW, Mitra S. Age- and tissue-specific changes in mitochondrial and nuclear DNA base excision repair activity in mice: Susceptibility of skeletal muscles to oxidative injury. Mech Ageing Dev. 131(5):330-7, 2010. PMID: 20363243

24. Zheng L, Dai H, Zhou M, Guo Z, Wu X, Wu J, Su L, Zhong X, Huang G, Kernstine K, Pfeifer G, Hegde ML, Mitra S and Shen B. FEN-1 mutations that specifically disrupt its interaction with PCNA cause aneuploidy-associated cancer. Cell Res, 2011, 21(7) 1052-1067

25. Theriot CA, Hegde ML, Hazra TK and Mitra S. (2010) RPA Physically Interacts With The Human DNA Glycosylase NEIL1 To Regulate Excision Of Oxidative DNA Base Damage In Primer-Template Structures. DNA Repair 9:642-652, 2010. PMID: 20338831

26. Bocangel, D., Sengupta, S., Mitra, S., and Bhakat, K. K. p53-Mediated down-regulation of the human DNA repair gene O6-methylguanine-DNA methyltransferase (MGMT) via interaction with Sp1 transcription factor. Anticancer Res., 29:3741-3750, 2009. PMID: 19846904

27. Barnes, T., Kim, W-C., Mantha, A. K., Kim, S-E., Izumi, T., Mitra, S. and Lee, C. H. Identification of apurinic/apyrimidinic endonuclease 1 (APE1) as the endoribonuclease that cleaves c-myc mRNA. Nucleic Acids Research 37: 3946-3958, 2009. PMID: 19401441 [PubMed - as supplied by publisher]

28. Bhattaracharyya, A., Chattopadhyay, R., Burnette, B. R., Cross,, J. V., Mitra, S., Ernst, P. B., Bhakat, K. K., and Crowe, S. E. Acetylation of Apurinic/Apyrimidinic Endonuclease-1 Regulates Helicobacter pylori-Mediated Gastric Epithelial Cell Apoptosis. Gastroenterology 136:2259-2269, 2009. PMCID: PMC2694750 [Available on 2010/06/01]

29. Vascotto, C., Cesaratto, L., Zeef, L. A., Deganuto, M., D'Ambrosio, C., Scaloni, A., Romanello, M., Damante, G., Taglialatela, G., Delneri, D., Kelley, M. R., Mitra, S., Quadrifoglio, F., and Tell, G. Genome-wide analysis and proteomic studies reveal APE1/Ref-1 multifunctional role in mammalian cells. Proteomics 9:1058-1074, 2009. PMID: 19180539 [PubMed - in process]

18











30. O'Hara, A. M., Bhattacharyya, A., Bai, J., Mifflin, R. C., Ernst, P. B., Mitra, S., and Crowe, S. E. Tumor necrosis factor (TNF)-induced IL-8 expression in gastric epithelial cells: Role of reactive oxygen species and AP endonuclease-1/redox factor (Ref)-1. Cytokine 46:359-369, 2009. PMID: 19376732

31. Hegde, M. L., Theriot, C. A., Das, A., Hegde, P. M., Guo, Z., Gary, R. K., Hazra, T. K., Shen, B., and Mitra, S. Physical and functional interaction between human oxidized base-specific DNA glycosylase NEIL1 and flap endonuclease 1. J. Biol. Chem. 283:27028-27037, 2008. PMCID: PMC2556012

32. Fantini, D., Vascotto, C., Deganuto, M., Bivi, N., Gustincich, S., Marcon, G., Quadrifoglio, F., Damante, G., Bhakat, K.K, Mitra, S., Tell, G. APE1/Ref-1 regulates PTEN expression mediated by Egr-1. Free Radic Res. 42: 20-29, 2008. PMCID: PMC2677450

33. Hegde, M., Hazra, T. K., and Mitra, S. Early steps in DNA base excision/single-strand interruption repair pathway in mammalian cells. Cell Res. 18:27-47, 2008. PMCID: PMC2692221; PMID: 18166975

34. Mantha, A. K., Oezguen, N., Bhakat, K. K., Izumi, T., Braun, W., and Mitra, S. Unusual role of a cysteine residue in substrate binding and activity of human AP-endonuclease 1. J. Mol. Biol. 379: 28-37, 2008. PMCID: PMC2708089

35. Szczesny, B., Tann, A. W., Longley, M. J., Copeland, W. C., and Mitra, S. Long patch base excision repair in mammalian mitochondrial genomes. J. Biol. Chem. 283:26349-26356, 2008. PMCID: PMC2546560

36. Maiti, A. K., Boldogh, I., Spratt, H., Mitra, S., and Hazra, T. K. Mutator phenotype of mammalian cells due to deficiency of NEIL1 DNA glycosylase, an oxidized base-specific repair enzyme. DNA Repair 7: 1213-1220, 2008.

37. Chattopadhyay R, Das, S., Maiti, A. K., Boldogh, I., Xie, J., Hazra, T. K., Kohno, K., Mitra, S., and Bhakat, K. K. Regulatory Role of Human AP-Endonuclease (APE1/Ref-1) in YB-1-Mediated Activation of the Multidrug Resistance Gene MDR1. Mol. Cell. Biol. 28:7066-7080, 2008. PMCID: PMC2593380; PMID: 18809583.

38. Adhikari, S., Kennel, S. J., Roy, G., Mitra, P. S., Mitra, S., and Roy, R.., Discrimination of lesion removal of N-methylpurine-DNA glycosylase revealed by a potent neutralizing monoclonal antibody. DNA Repair 7:31-39, 2008.

39. Dou, H., Theriot, C. A., Das, A., Hegde, M. L., Matsumoto, M., Boldogh, B., Hazra, T. K., Bhakat, K. K., and Mitra, S. Interaction of the Human DNA Glycosylase NEIL1 with Proliferating Cell Nuclear Antigen: The Potential for Replication-Associated Repair of Oxidized Bases in Mammalian Genome. J. Biol. Chem. 283: 3130-3140, 2008.

40. Zaky, A., Busso, C., Izumi, T., Chattopadhyay, R., Bassiouny, A., Mitra, S. and Bhakat, K. K. Regulation of the human AP-endonuclease (APE1/Ref-1) expression by the tumor suppressor p53 in response to DNA damage. Nucleic Acid Res. 36: 1555-1566, 2008. PMCID: PMC2275136

41. Hegde, M. L., Hazra, T. K., and Mitra, S. Early steps in the DNA base excision /single-strand interruption repair pathway in mammalian cells. Cell Research. 18:27-47, 2008. PMCID: PMC2692221

42. Das A, Boldogh I, Lee J W, Harrigan J, Hegde ML, Piotrowski J, Souza-Pinto N, Ramos W, Greenberg M, Hazra TK, Mitra S, Bohr V. The human Werner syndrome protein stimulates repair of oxidative DNA base damage by the DNA glycosylase NEIL1. J. Biol. Chem. 282:26591-26602, 2007.

19

http://www.pubmedcentral.gov/articlerender.fcgi?tool=nihms&artid=2692271

43. Mitra, S., Izumi, T., Boldogh, I., Bhakat, K. K., Chattopadhyay, R. and Szczesny, B. Intracellular Trafficking and Regulation of Mammalian AP-endonuclease 1 (APE1), An Essential DNA Repair Protein. DNA Repair Special Issue. 6:461-469, 2007.

44. Mitra, S. MGMT: A personal perspective. DNA Repair 6:1064:1070, 2007.

45. Bacsi, A., Woodberry, M., Widger, W., Papaconstantinou, J., Mitra, S., Peterson, J.W., Boldogh, I. Localization of superoxide anion production to mitochondrial electron transport chain in 3-NPA-treated cells. Mitochondrion 6:235 – 244, 2006.

46. Guan, X., Bai, H., Shi, G., Theriot, C. A., Hazra, T. K., Mitra, S., and Lu, A-L. The human checkpoint sensor Rad9-Rad1-Hus1 interacts with and stimulates NEIL1 glycosylase. Nucleic Acids Research 35:2463-2472, 2007.

47. Bhakat, K. K., Mokkapati, S. K., Boldogh, I., Hazra, T. K., and Mitra, S. Acetylation of human 8-oxoguanine-DNA glycosylase by p300 and its role in 8-oxoguanine repair in vivo. Mol. Cell. Biol. 2006; 26:1654-1665.

48. Das, A., Wiederhold, L., Leppard, J. B., Kedar, P., Prasad, R., Wang, H., Boldogh, I., Karimi-Busheri, F., Weinfeld, M., Tomkinson, A. E., Wilson, S. H., Mitra, S., and Hazra, T. K. NEIL2-initiated, APE-independent repair of oxidized bases in DNA: Evidence for a repair complex in human cells. DNA Repair (Amst) 5:1439-1448, 2006.

49. O'Hara, A. M, Bhattacharyya, A., Mifflin, R. C., Smith, M. F., Ryan, K. A., Scott, K. G-E., Naganuma, M., Casola, A., Izumi, T., Mitra, S., Ernst, P. B. and Crowe, S. E.. Interleukin-8 induction by helicobacter pylori in gastric epithelial cells is dependent on apurinic/apyrimidinic endonuclease-1/redox factor-11. J. of Immunology 177:7990-7999, 2006.

50. Chattopadhyay, R., Wiederhold, L., Szczesny, B., Boldogh, I., Hazra, T. K., Izumi, T., and Mitra, S. Identification and characterization of mitochondrial abasic (AP)-endonuclease in mammalian cells. Nucleic Acids Res 34:2067-2076, 2006.

51. Das, A., Hazra, T. K., Boldogh, I., Mitra, S., and Bhakat, K. K. Induction of the human oxidized base-specific DNA glycosylase NEIL1 by reactive oxygen species. J. Biol. Chem. 280:35272-35280, 2005.

52. Boldogh, I., Bacsi, A., Choudhury, B. K., Dharajiya, N., Alam, R., Hazra, T. K., Mitra, S., Goldblum, R. M. and Sur, S. ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation. J. Clin. Invest.115:2169-2179, 2005.

53. Jackson, E. B., Theriot, T. A., Chattopadhyay, R., Mitra, S., and Izumi, T. Analysis of nuclear transport signals in the human apurinic/apyrimidinic endonuclease (APE1/Ref1). Nucleic Acids Research 33:3303-3312, 2005.

54. Szczesny, B. and Mitra, S. Effect of aging on intracellular distribution of abasic (AP) endonuclease 1 in the mouse liver. Mech. Ageing Dev. 126:1071-1078, 2005.

55. Izumi, T., Brown, D. B., Naidu, C. V., Bhakat, K. K., MacInnes, M. A., Saito, H., Chen|, D. J. and Mitra, S. Two essential but distinct functions of the mammalian AP-endonuclease. Proc. Natl. Acad. Sci. 102:5739-5743, 2005.

56. Das, A., Rajagopalan, L., Mathura, V. S., Rigby, S. J., Mitra, S. and Hazra, T. K. Identification of a zinc finger domain in the human NEIL (Nei like)-2 protein. J. Biol. Chem., 279, 47132-47138, 2004.

57. Mokkapati, S. K., Wiederhold, L., Hazra, T. K., and Mitra, S. Stimulation of DNA glycosylase activity of OGG1 by NEIL1: Functional collaboration between two Human DNA glycosylases.

20

Biochemistry. 43:11596-11604, 2004.

58. Wiederhold, L., Leppard, J. B., Kedar, P., Karimi-Busheri, F., Rasouli-Nia, A., Weinfeld, M., Tomkinson, A. E., Izumi, T., Prasad, R., Wilson, S. H., Mitra, S., and Hazra, T. K. AP Endonuclease-independent DNA Base Excision Repair in Human Cells. Molecular Cell 15, 209-220, 2004.

59. Szczesny, B., Bhakat, K. K., Mitra, S., and Boldogh, I. “Age-dependent modulation of DNA repair enzymes by covalent modification and subcellular distribution.” Mech. Ageing Dev. 125, 755-765, 2004.

60. Ding, S-Z., O’Hara, A. M., Denning, T. L., Dirden-Kramer, B., Mifflin, R. C., Reyes, V. E., Ryan, K. A., Elliott, S. N., Izumi, t., Boldogh, I., Mitra, S., Ernst, P. B., and Crowe, S. E. Helicobacter pylori and H2O2 increase AP endonuclease-1/redox factor-1 expression in human gastric epithelial cells. Gastroenterology 127:845-858, 2004.

61. Bhakat, K. K., Hazra, T. K. and Mitra, S. Acetylation of the Human DNA Glycosylase NEIL2 and Inhibition of Its Activity. Nucleic Acids Res. 32, 3033-3039, 2004.

62. Ribar, B., Izumi, T. and Mitra, S. The major role of human AP-endonuclease homolog Apn2 in repair of abasic sites in Scizosaccharomyces pombe. Nucleic Acids Res. 32, 115-126, 2004.

63. Dou, H., Mitra, S. and Tapas K. Hazra, T. K. Repair of oxidized bases in DNA bubble structures by human DNA glycosylases NEIL1 and NEIL2. J. Biol. Chem. 278:49679-49684, 2003.

64. Bhakat, K. K., Izumi, T., Yang, S. H., Hazra, T. K. and Mitra, S. Role of acetylated human AP-endonuclease (APE1/Ref-1) in regulation of the parathyroid hormone gene, EMBO J. 22:6299-6309, 2003.

65. Szczesny, B., Hazra, T. K., Papaconstantinou, J., Mitra, S. and Boldogh, I. Age-dependent deficiency in import of mitochondrial DNA glycosylases required for repair of oxidatively damaged bases. PNAS 100:10670-10675, 2003. *(Special press release by PNAS.)

66. Boldogh, I., Roy, G., Lee, M-S., Bacsi,A., Hazra, T. K., Bhakat, K. B., Das,G. C., and Mitra, S. Reduced DNA double strand breaks in chlorambucil resistant cells are related to high DNA-PKcs activity and low oxidative stress. Toxicology 193:137-152, 2003.

67. O'Hara, A. M, Ding, S. Z., Izumi, T., Mitra, S., Mifflin, R. C., and Crowe, S. E. La role du stress oxydatif dans I’infection a helicobacter pylori. ILa Lettre de I’Infectiologue 13:8-13, 2003.

68. Bhakat, K. K. and Mitra, S. CpG Methylation-Dependent Repression of the Human 06–Methylguanine-DNA Methyltransferase Gene Linked to Chromatin Structure Alteration. Carcinogenesis 24:1337-1345, 2003.

69. Hazra, T. K., Izumi, T., Kow, Y. W., and Mitra, S. The discovery of a new family of mammalian enzymes for repair of oxidatively damaged DNA, and its physiological implications. Carcinogenesis 24:155-157, 2003.

70. Thiviyanathan, V., Somasunderam, A., Hazra, T. K., Mitra, S., and Gorenstein, D. G. Solution structure of a DNA duplex containing 8-hydroxy-2'-deoxyguanosine opposite deoxyguanosine, J. Mol. Biol. 325:433-442, 2003.

71. Izumi, T., Wiederhold, L. R., Roy, G., Roy, R., Jaiswal, A., Bhakat, K. K., Mitra, S., and Hazra, T. K. Mammalian DNA base excision repair proteins: their interactions and role in repair of oxidative DNA damage. Toxicology 193:43-65, 2003

21

72. Mitra, S., Izumi, T., Boldogh, I., Bhakat, K., Hill, J. W., and Hazra, T. K. Choreography of oxidative DNA damage repair in mammalian cells involving distinct protein ensembles. Invited Article in Free Radicals in Molecular Biology, 33:15-28, 2002.

73. Bocangel, D. B., Finkelstein, S., Schold, S. C., Bhakat, K. K., Mitra, S., and Kokkinakis, D. M. Multifaceted resistance of gliomas to temozolomide. Clin. Cancer Res. 8:2725-2734, 2002.

74. Biswas, T., Clos II L. J., SantaLucia, J. Jr., Mitra, S., and Roy, R. Binding of Specific DNA Base Pair Mismatches by N-Methylpurine-DNA Glycosylase and its Implication in Initial Damage Recognition. J. Mol. Biol. 320:503-513, 2002.

75. Hazra, T. K., Kow, Y. W., Hatahet, Z., Imhoff, B., Boldogh, I., Mokkapati, S. K., Mitra, S., and Izumi, T. Identification and characterization of a novel human DNA glycosylase for repair of cytosine-derived lesions. J. Biol. Chem., 277:30417-30420, 2002.

76. Hazra, T. K.; Izumi, T.; Boldogh, I.; Imhoff, B.; Kow, Y. W.; Jaruga, P.; Dizdaroglu, M. Identification and characterization of a human DNA glycosylase for repair in modified bases in oxidatively damaged DNA. PNAS, 99:3523-3528, 2002*. (*Special citation by the journal)

77. Mitra, S., Izumi, T., Boldogh, I., Bhakat, K. K., Hill, J. W., and Hazra, T. K. Choreography of oxidative damage repair in mammalian genomes. Free Rad. Biol. Med. 33:15-28, 2002.

78. Kuninger, D. T., Izumi, T., Papaconstantinou, J., and Mitra, S. Human AP-endonuclease 1 and hnRNP-L interact with a nCaRE-like repressor element in the AP-endonuclease-1 promoter. Nucleic Acids Res., 30:823-829, 2002.

79. Hill, J. W., Hazra, T. K., Izumi, T., and Mitra, S. 2001. Stimulation of human 8-oxoguanine-DNA glycosylase by AP-endonuclease: potential coordination of the initial steps in base excision repair. Nucleic Acids Res. 29:430-438.

80. Mitra, S., Boldogh, I., Izumi, T., and Hazra, T. K. Complexities of the DNA base excision repair pathway for repair of oxidative DNA damage. Invited Article In: Environmental and Molecular Mutagenesis (Special Issue) 38:180-190, 2001.

81. Hazra, T. K., Muller, J. G., Manuel, R. C., Burrows, C. J., Lloyd, R. S. and Mitra, S. Repair of hydantoins, one electron oxidation product of 8-oxoguanine, by DNA glycosylases of Escherichia coli. Nucleic Acids Res. 29, 1967-1974, 2001.

82. Bhakat, K. K., and Mitra, S. Regulation of the human o6-methylguanine-DNA methyltransferase gene by transcriptional coactivators cAMP response element-binding protein-binding protein and p300. J. Biol. Chem. 275, 34197-34204, 2000.

83. Hazra, T. K., Izumi, T., Venkataraman, R., Kow, Y. K., Dizdaroglu, M., and Mitra, S. Characterization of a novel 8-oxoguanine-DNA glycosylase activity in E. coli and identification of the enzyme as endonuclease VIII. J. Biol. Chem. 275:27762-27767, 2000.

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84. Wang, G., Hazra, T. K., Mitra, S., Lee, H-M., and Englander, E. W. Mitochondrial DNA damage and a hypoxic response are induced by CoCl2 in rat neuronal PC12 cells. Nucl. Acids Res., 28:2135-2140, 2000.

85. Izumi, T., Hazra, T. K., Boldogh, I., Tomkinson, A. E., Park, M. S., Ikeda, S., and Mitra, S. Requirement of human AP-endonuclease 1 for repair of 3-blocking damage at DNA single-strand breaks induced by reactive oxygen species. Carcinogenesis 21:1329-1334, 2000.

86. Mol. C.D., Izumi, T., Mitra, S. and Tainer, J.A. DNA-bound structures and mutants reveal abasic DNA binding by APE1 DNA repair and coordination. Nature 403:451–456, 2000.(Special commentary in Nature).

87. Roy, R., Biswas, T., Lee, J. C., and Mitra, S. Mutation of a unique aspartate residue abolishes the catalytic activity but not substrate binding of the mouse N-methylpurine-DNA glycosylase (MPG). J. Biol. Chem. 275:4278-4282, 2000.

88. Roy, G., Horton, J. K., Roy, R., Denning, T., Mitra, S., and Boldogh, I. Acquired alkylating drug resistance of a human ovarian carcinoma cell line is unaffected by altered levels of pro- and anti-apoptotic proteins. Oncogene 19:141-150, 2000.

89. Izumi, T., Malecki, J., Chaudhry, M. A., Weinfeld, M., Hill, J. H., Lee, J. C., and Mitra, S. Intragenic suppression of an active site mutation in the human apurinic/apyrimidinic endonuclease. J. Mol. Biol. 287:47-57, 1999.

90. Biswas, T., Ramana, C. V., Srinivasan, G., Boldogh, I., Hazra, T. K., Chen, Z., Tano, K., Thompson, E. B., and Mitra, S. Activation of human O6-methylguanine-DNA methyltransferase gene by glucocorticoid hormone. Oncogene. 18:525-532, 1999.

91. Horton, J. K., Roy, G., Piper, J. T., Awasthi, Y. C., Mitra, S., Alaoui-Jamali, Boldogh, I., and Singhal, S. Characterization of a chlorambucil-resistant human ovarian carcinoma cell line overexpressing glutathione S-transferase . Biochemical Pharmacology. 58:693-702, 1999.

92. Edwards, M., Kent, T. A., Rea, H. C., Wei, J., Quast, M., Izumi T., Mitra, S., and Perez-Polo, J. R. APE/Ref-1 responses to ischemia in rat brain. Neuroreport. 9:4015-4018, 1998.

93. Edwards, M., Rassin, D. K., Izumi, T., Mitra, S., and Perez-Polo, J. R. APE/Ref-1 responses to oxidative stress in aged rats. J. Neurosci. Res. 54:635-638, 1998.

94. Hazra, T. K., Izumi, T., Maidt, L., Floyd, R. A., and Mitra, S. The presence of two distinct 8-oxoguanine repair enzymes in human cells: Their potential complementary roles in preventing mutation. Nucleic Acids Research 26:5116-5122, 1998.

95. Boldogh, I., Ramana, C. V., Chen, Z., Biswas, T., Hazra, T. K., Grosch, S., Grombacher, T., Mitra, S., and Kaina, B. Regulation of expression of the DNA repair gene O6-methylguanine-DNA methyltransferase via protein kinase C-mediated signaling. Cancer Research 58:3950-3956, 1998.

96. Johnson, R. E., Torres-Ramos, C. A., Izumi, T., Mitra, S., Prakash, S., and Prakash, L. Identification of APN2, the Saccharomyces cerevisiae homolog of the major human AP-endonuclease HAP1, and its role in the repair of abasic sites. Genes & Dev. 12:3137-3143, 1998.

97. Ikeda, S., Biswas, T., Roy, R., Izumi, T., Boldogh, I., Kurosky, A., Sarker, A., Seki, S., and Mitra, S. Purification and characterization of hNTH1, a human homolog of Escherichia coli endonuclease III: direct identification of Lys-212 as the active nucleophilic residue. J. Biol. Chem. 273: 21585-21593, 1998.

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98. Ramana, C. V., Boldogh, I., Izumi, T., and Mitra, S. Activation of AP-endonuclease in human cells by reactive oxygen species and its correlation with their adaptive response to genotoxicity of free radicals. Proc. Natl. Acad. Sci . USA, 95:5061-5066, 1998.

99. Bhattacharyya, D., Hazra, T. K., Behnke, W. D., Chong, P.-S., Kurosky, A., Lee, J. C., and Mitra, S. Reversible folding of ADA Protein (O 6-methylguanine-DNA methyltransferase) of Escherichia coli . Biochemistry, 37:1722-1730, 1998.

100. Izumi, T. and Mitra, S. Deletion analysis of human AP-endonuclease: Minimum sequence required for the endonuclease activity. Carcinogenesis, 19:525-527, 1998.

101. Roy, R., Biswas, T., Hazra, T. K., Roy, G., Grabowski, D. T., Izumi, T., Srinivasan, G., and Mitra, S. Specific interaction of wild type and truncated mouse N-methylpurine-DNA glycosylase with ethenoadenine-containing DNA. Biochemistry. 37:580-589, 1998.

102. Izumi, T., Tano, K., Tatsuka, M., and Mitra, S. Molecular cloning and characterization of the promoter of the human N-methylpurine-DNA glycosylase (MPG) gene. Carcinogenesis 18:1837-1839, 1997.

103. Hazra, T. K., R. Roy, T. Biswas, D. T. Grabowski, A. E. Pegg, and S. Mitra.. Specific recognition of O 6-methylguanine in DNA by active site mutants of human O 6-methylguanine-DNA methyltransferase. Biochem. 36:5769-5776, 1997.

104. Tano, K., Dunn, W. C., Darroudi, F., Shiota, S., Preston, R. J., Natarajan, A. T., and Mitra, S. Amplification of the DNA repair gene O 6-methylguanine-DNA methyltransferase associated with resistance to alkylating drugs in a mammalian cell line. J. Biol. Chem. 272:13250-13254, 1997.

105. Roy, G., Roy, R., and Mitra, S. Quantitative reverse transcriptase polymerase chain reaction for measuring the N-methylpurine-DNA glycosylase mRNA level in rodent cells. Analytical Biochem. 246:45-51, 1997.

106. Grombacher, T., Mitra, S., and Kaina, B. Induction of the alkyltransferase (MGMT) gene by DNA damaging agents and the glucocorticoid dexamethasone and comparison with the response of base excision repair genes. Carcinogenesis. 17:2329, 2336., 1996

107. Roy, R., Kumar, A., Lee, J. C., and Mitra, S. The domains of mammalian base excision repair enzyme N-methylpurine-DNA glycosylase: Interaction, conformational change and role in DNA-binding and damage recognition. J. Biol. Chem. 271:23690-23697, 1996.

108. Roy, R., Kennel, S. J., and Mitra, S. Distinct substrate preference of human and mouse N-methylpurine-DNA glycosylases. Carcinogenesis 17:2177-2182, 1996.

109. Zhao, Q. Z., Liang, X. L., Mitra, S., Gourdon, G., and Alter, B. P. Cloning and characterization of the mouse alpha globin cluster and a new hypervariable marker. Mammalian Genome 7:749-753, 1996.

110. Izumi, T., Henner, W. D., and Mitra, S. Negative regulation of the major human AP-endonuclease, a multifunctional protein. Biochemistry 35:14679-14683, 1996.

111. Yen, L., Woo, A., Christopoulopoulos, G., Batist, G., Panasci, L., Roy, R., Mitra, S., and Alaoui-Jamali, M. A. Enhanced host cell reactivation capacity and expression of DNA repair genes in human breast cancer cells resistant to bi-functional alkylating agents. Mutation Res. 337: 179-190, 1995.

112. Tatsuka, M., Ibeanu, G. C., Izumi, T., Narayan, S., Kim, N. K., Kang, W., Roy, G., and Mitra, S. Structural organization of mouse DNA repair gene, N-methylpurine-DNA glycosylase (MPG).

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DNA and Cell Biol. 14: 37-45, 1995.

113. Roy, R., Shiota, S., Kennel, S. J., Raha, R., von Wronski, M., Brent, T. P., and Mitra, S. A comparative study of the biochemical properties of human and mouse recombinant O6-methylguanine-DNA methyltransferases. Carcinogenesis 16: 405-411, 1995.

114. Kato, Jr., T., Todo, T., Ayaki, H., Ishizaki, K., Morita, T., Mitra, S., and Ikenaga, M. Cloning of a marsupial DNA photolyase gene and lack of a related sequence in placental mammals. Nucl. Acids Res. 22: 4119-4124, 1994.

115. Roy, R., Brooks, C., and Mitra, S. Purification and biochemical characterization of recombinant N-methylpurine-DNA glycosylase of the mouse. Biochemistry. 33: 15131-15140, 1994.

116. Dosanjh, M. K., Roy, R., Mitra, S. and Singer, B. 1,N 6-ethenoadenine is preferred over 3-methyladenine as substrate by a cloned human N-methylpurine-DNA glycosylase (3-methyladenine-DNA glycosylase, MPG). Biochemistry. 33: 1624-1628, 1994.

117. Bessho, T., Roy, R., Yamamoto, K., Kasai, H., Nishimura, S., Tano, K., and Mitra, S. Repair of 8-hydroxyguanine in DNA by mammalian N-methylpurine-DNA glycosylase. Proc. Natl. Acad. Sci. USA. 90: 8901-8904, 1993.

118. Walter, C. A., Lu, J., Bhakta, M., Mitra, S., Dunn, W., Herbert, D. C., Weaker, F. J., Toog, T., Garza, P., Adrian, G. S., and Kamolvarin, N. Brain and Liver targeted Overexpression of O6-methylguanine DNA Methyltransferase in Transgenic Mice. Carcinogenesis 14: 1537-1543, 1993.

119. von Wronski, M. A., Harris, L. C., Tano, K., Mitra, S., Bigner, D. D., and Brent, T. P. Cytosine methylation and suppression of O6-methylguanine-DNA methyltransferase expression in human rhabdomyosarcoma cell lines and xenographs. Oncology Research. 4: 167-174, 1992.

120. Ibeanu, G., Hartenstein, B., Dunn, W. C., Chang, L-Y, Hofmann, E., Coquerelle, T., Mitra, S., and Kaina, B. Overexpression of human DNA repair protein N-methylpurine-DNA glycosylase results in the increased removal of N-methylpurines in DNA without a concomitant increase in resistance to alkylating agents in Chinese hamster ovary cells. Carcinogenesis. 13: 1989-1995, 1992.

121. He, X., Ostrowski, L. E., von Wronski, M. A., Friedman, H. S., Wikstrand, C. J., Bigner, S. H., Rasheed, A., Batra, S. K., Mitra, S., Brent, T. P. and Bigner, D. D. Expression of O6-methylguanine-DNA methyltransferase in six well characterized human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992.

122. Shiota, S., von Wronski, M., Tano, K., Bigner, D. D., Brent, T. P., and Mitra, S. Characterization of cDNA encoding mouse DNA repair protein O6 -methylguanine-DNA methyltransferase: Structure and high level expression of the wild type and mutant proteins in E. coli. Biochemistry 31: 1897-1903, 1992.

123. Ostrowski, L. E., Bigner, S. H., von Wronski, M. A., Rasheed, A., Schold, S. C., Brent, T. P., Mitra, S. and Bigner, D. D. Expression of O6-methylguanine-DNA methyltransferase in malignant human glioma cell lines. Carcinogenesis 12: 1739-1744, 1991.

124. Wang, Y., Kato, T., Ayaki, H., Ishizaki, K., Tano, K., Mitra, S. and Ikenaga, M. Correlation between DNA hypomethylation and expression of O6-methylguanine-DNA methyltransferase gene in cultured human tumor cells. Mutat. Res. 273: 221-230, 1992.

125. Natarajan, A. T., Valentine, M., Brent, T. P., Darroudi, F., Mitra, S., and Tano, K. Chromosomal localization of human O6-methylguanine-DNA methyltransferase by in situ hybridization. Mutagenesis 7: 83-85, 1992.

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126. Kaina, B., Fritz, G., Mitra, S. and Coquerelle, T. Transfection and expression of human O6-methylguanine-DNA methyltransferase (MGMT) in Chinese hamster cells: The role of MGMT in protection against genotoxic effects of alkylating agents. Carcinogenesis 12: 1857-1867, 1991.

127. Harris, L. C., Potter, P. M., Tano, K., Shiota, S., Mitra, S. and Brent, T. P. Characterization of the promoter region of the human O6-methylguanine-DNA methyltransferase gene. Nucl. Acids Res. 19: 6163-6167, 1991.

128. Fritz, G., Tano, K., Mitra, S. and Kaina, B. Induction of the O6-methylguanine-DNA methyltransferase gene in rat liver cells by DNA damaging treatments. Mol. Cell. Biol. 11: 4660-4668, 1991.

129. Ostrowski, L. E., Pegram, C. N., von Wronski, M., Humphrey, P. A., He Xuanmin, Shiota, S., Mitra, S., Brent, T. P. and Bigner, D. D. Production and characterization of antipeptide antibodies against human O6-methylguanine-DNA methyltransferase. Cancer Res. 51: 3339-3344, 1991.

130. Waters, L. C., Sikpi, M. O., Preston, R. J., Mitra, S. and Jaberaboansari, A. Mutations induced by ionizing radiation in a plasmid replicated in human cells. Similar, nonrandom distribution of mutations in unirradiated and X-irradiated DNA. Radiat. Res. 127: 190-201, 1991.

131. Tano, K., Shiota, S., Remack, J. S., Brent, T. P., Bigner, D. D. and Mitra, S. The origin of O6-methylguanine-DNA methyltransferase in Chinese hamster ovary cells transfected with human DNA. Mutat. Res. 255: 175-182, 1991.

132. Chakravarti, D., Ibeanu, G., Tano, K. and Mitra, S. Cloning and expression in E. coli of a cDNA encoding the human repair protein for N-alkylpurines in DNA. J. Biol. Chem. 266: 15710-15715, 1991.

133. Jaberaboansari, A., Dunn, W. C., Preston, R. J., Mitra, S. and Waters, L. C. Sequence analysis of alpha particle-induced point mutations in a plasmid replicated in human cells. Radiat. Res. 127: 202-210, 1991.

134. Dunn, W. C., Tano, K., Horesovsky, G., Preston, R. J. and Mitra, S. The role of 06-alkylguanine in cell killing and mutagenesis in Chinese hamster ovary cells. Carcinogenesis 12: 83-89, 1991.

135. von Wronski, M. A., Shiota, S., Tano, K., Mitra, S., Bigner, D. D. and Brent, T. P. Structural and immunological comparison of indigenous human O6-methylguanine-DNA methyltransferase with that encoded by a cloned cDNA. J. Biol. Chem. 266: 1064-1070, 1991.

136. Bhattacharyya, D., Foote, R. S., Boulden, A. M. and Mitra. S. Physicochemical studies of human 06-methylguanine-DNA methyltransferase. Eur. J. Biochem. 193: 337-343, 1990.

137. Tano, K., Shiota, S., Collier, J., Foote, R. S. and Mitra, S. Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine. Proc. Natl. Acad. Sci. USA 87: 686-690, 1990.

138. Sikpi, M. O., Waters, L. C., Kraemer, K. H., Preston, R. J. and Mitra. S. N-methyl-N-nitrosourea-induced mutations in a shuttle plasmid replicated in human cells. Mol. Carcin. 3: 30-36, 1990.

139. Tano, K., Bhattacharyya, D., Foote, R. S., Mural, R. J. and Mitra, S. Site-directed mutation of E. coli ada gene. Effects of substitution on methyl acceptor cysteine-321 by histidine in Ada protein. J. Bacteriol. 171: 1535-1543, 1989.

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140. Washington, W. J., Foote, R. S., Dunn, W. C., Generoso, W. M. and Mitra. S. Age-dependent modulation of tissue-specific repair activity for 3-methyladenine and 06-methylguanine in DNA in inbred mice. Mech. Ageing and Dev. 48: 43-52, 1989.

141. Schold, S. C., Brent, T. P., von Hofe, E., Friedman, H. S., Mitra, S., Bigner, D. D., Swenberg, J. A. and Kleihues, P. 06-alkylguanine-DNA alkyltransferase and sensitivity to procarbazine in human brain tumor xenografts. J. Neurosurg. 70: 573-577, 1989.

142. Foote, R. S., Dunn, W. C., Stankowski, L. F., Hsie, A. W. and Mitra, S. Mutagenic potential of modified DNA precursors in vivo: Fate of 06-methyldeoxyguanosine triphosphate in Chinese hamster ovary cells. Indian J. Biochem. Biophys. 25: 472-477, 1988 (Silver Jubilee Issue).

143. Bhattacharyya, D., Boulden, A. M., Foote, R. S. and Mitra, S. Effect of polyvalent metal ions on the reactivity of human 06-methylguanine-DNA methyltransferase. Carcinogenesis 9: 683-685, 1988.

144. Bhattacharyya, D., Tano, K., Bunick, G. J., Uberbacher, E. C., Behnke, W. D. and Mitra, S. Rapid, large-scale purification and characterization of "Ada protein" (06-methylguanine-DNA methyltransferase) of E. coli. Nucl. Acids Res. 16: 6397-6410, 1988.

145. Washington, W. J., Dunn, W. C., Generoso, W. M. and Mitra, S. Tissue-specific variation in repair activity for 3-methyladenine in DNA in two stocks of mice. Mutat. Res. 207: 165-169, 1988.

146. Tano, K., Foote, R. S. and Mitra, S. High-level expression of the cloned ada gene of E. coli by deletion of its regulatory sequence. Gene 64: 305-311, 1988.

147. Boulden, A. M., Foote, R. S., Fleming, G. S. and Mitra, S. Purification and some properties of human DNA-06-methylguanine methyltransferase. J. Biosci. 11: 215-224, 1987.

148. Hartman, F. C., Soper, T. S., Niyogi, S. K., Mural, R. J., Foote, R. S. Mitra, S., Lee, E. H., Machanoff, R. and Larimer, F. W. Function of lys-166 of R. rubrum ribulosebisphosphate carboxylase/oxygenase as examined by site-directed mutagenesis. J.Biol. Chem. 262: 3496-3501, 1987.

149. Niyogi, S. K., Soper, T. S., Foote, R. S., Larimer, F. W., Mural, R. J., Mitra, S., Lee, E. H., Machanoff, R. and Hartman, F. C. Site-directed mutagenesis to determine essential residues of ribulosebisphosphate carboxylase of Rhodospirillum rubrum. J. Biosci. 11: 203-214, 1987.

150. Niyogi, S. K., Foote, R. S., Mural, R. J., Larimer, F. W., Mitra, S., Soper, T. A., Machanoff, R. and Hartman, F. C. Nonessentiality of His-291 of R. rubrum ribulose bisphosphate carboxylase/oxygenase as determined by site-directed mutagenesis. J. Biol. Chem. 261: 10087-10092, 1986.

151. Dunn, W. C., Foote, R. S., Hand, Jr., R. E. and Mitra, S. Cell cycle-specific synthesis of 06-methylguanine-DNA methyltransferase in C3H T10 1/2 mouse cells. Carcinogenesis 7: 807-812, 1986.

152. Wobbe, C. R. and Mitra, S. Proteins tightly associated with the termini of replicative form DNA of Kilham rat virus, an autonomous parvovirus. Proc. Natl. Acad. Sci. USA 82: 8335-8339, 1985.

153. Bhattacharyya, A., Mitra, S. and Pal, B. C. Synthesis of 02 and 04-ethylthymidine 5'-triphosphates. Nucleosides and Nucleotides 3: 353-361, 1984.

154. Yarosh, D. B., Rice, M., Day III, R. S., Foote, R. S. and Mitra, S. MerC human tumor cells lack 06-methylguanine-DNA methyltransferase. Mutat. Res. 131: 27-36, 1984.

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155. Bates, R. C., Snyder, C. E., Banerjee, P. T. and Mitra, S. Autonomous parvovirus LUIII encapsidates equal amounts of plus and minus DNA strands. J. Virol. 49: 319-324, 1984.

156. Foote, R. S. and Mitra, S. Lack of induction of 06-methylguanine-DNA methyltransferase in mammalian cells treated with N-methyl-N'-nitro-N-nitrosoguanidine. Carcinogenesis 5: 277-281, 1984.

157. Snow, E. T., Foote, R. S. and Mitra, S. Base pairing properties of 06-methylguanine in template DNA during in vitro DNA replication. J. Biol. Chem. 259: 8095-8100, 1984.

158. Snow, E. T., Foote, R. S. and Mitra, S. Kinetics of incorporation of 06-methyldeoxyguanosine monophosphate during in vitro DNA synthesis. Biochemistry 23: 4289-4294, 1984.

159. Snow, E. T., Foote, R. S., Dodson, L. A., Masker, W. and Mitra, S. Mutagenic properties of 06-methylguanine in DNA in vivo and in vitro. Proc. XV. Internat. Cong. Genetics, pp. 291-302, 1984.

160. Wobbe, C. R., Mitra, S. and Ramakrishnan, V. The structure of the capsid of Kilham rat virus from small angle neutron scattering. Biochemistry 23: 6565-6569, 1984.

161. Banerjee, P. T., Olson, W. H., Allison, D. P., Bates, R. C., Snyder, C. E. and Mitra, S. Electron microscopic comparison of the sequences of single-stranded genomes of mammalian parvoviruses by heteroduplex mapping. J. Mol. Biol. 166: 257-272, 1983.

162. Burd, P. R., Mitra, S., Bates, R. C., Snyder, C. E., Thompson, L. D. and Stout, E. R. Restriction enzyme analysis of the bovine parvovirus genome. J. Gen. Virol. 64: 2521-2526, 1983.

163. Foote, R. S., Pal, B. C. and Mitra, S. Quantitation of 06-methylguanine-DNA methyltransferase in HeLa cells. Mutat. Res. 119: 221-228, 1983.

164. Hadden, C. T., Foote, R. S. and Mitra, S. Adaptive response of Bacillus subtilis to N-methyl-N'-nitro-N-nitrosoguanidine. J. Bacteriol. 153: 756-762, 1983.

165. Yarosh, D. B., Foote, R. S., Mitra, S. and Day III, R. S. Repair of 06-methylguanine in DNA by demethylation is lacking in MerC human tumor cell strains. Carcinogenesis 4: 199-205, 1983.

166. Pegg, A. E., Weist, L., Foote, R. S., Mitra, S. and Perry. W. Purification and properties of 06-methylguanine-DNA transmethylase from rat liver. J. Biol. Chem. 258: 2327-2333, 1983.

167. Snow, E. T., Foote, R. S. and Mitra, S. Replication and repair of 06-methylguanine in DNA. Prog. Nucleic Acids Res. Mol. Biol. 29: 99-103, 1983.

168. Dodson, L. A., Foote, R. S., Mitra, S. and Masker, W. E. Mutagenesis of bacteriophage T7 in vitro by incorporation of 06-methylguanine during DNA synthesis. Proc. Natl. Acad. Sci. USA 79: 7440-7444, 1982.

169. Pegg, A. E., Roberfroid, M., von Bahr, C., Foote, R. S., Mitra, S., Bresil, H., Likhachev, A. and Montesano, R. Removal from DNA of 06-methylguanine by human liver fractions. Proc. Natl. Acad. Sci. USA 79: 5162-5165, 1982.

170. Snyder, C. E., Schmoyer, R. L., Bates, R. C. and Mitra, S. Calibration of denaturing agarose gels for molecular weight estimation of DNA: Size determination of the single-stranded genomes of parvoviruses. Electrophoresis 3: 210-213, 1982.

171. Mitra, S., Bates, R. C., Snyder, C. E. and Banerjee, P. T. Comparative physicochemical and biological properties of two strains of Kilham rat virus. J. Gen. Virol. 61: 43-54, 1982.

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172. Mitra, S., Pal, B. C. and Foote, R. S. 06-methylguanine-DNA methyltransferase in wild type and ada mutants of Escherichia coli. J. Bacteriol. 152: 534-537, 1982.

173. Banerjee, P. T., Rothrock, R. and Mitra, S. Restriction map of the single-stranded DNA genome of Kilham rat virus strain 171, a nondefective parvovirus. J. Virol. 40: 118-125, 1981.

174. Foote, R. S., Mitra, S. and Pal, B. C. Demethylation of 06-methylguanine in a synthetic DNA polymer by an inducible activity in Escherichia coli. Biochem. Biophys. Res. Commun. 97: 654-659, 1980.

175. Dasgupta, S. and Mitra, S. Structure of nascent replicative form DNA of coliphage M13. Proc. Natl. Acad. Sci. USA 75: 153-157, 1978.

176. Niyogi, S. K. and Mitra, S. Comparison of specific binding sites for Escherichia coli RNA polymerase with naturally occurring hairpin regions in single-stranded DNA of coliphage M13. J. Biol. Chem. 253: 5562-5567, 1978.

177. Niyogi, S. K. and Mitra, S. Isolation and characterization of naturally occurring hairpin structures in single-stranded DNA of coliphage M13. Biochem. Biophys. Res. Commun. 79: 1037-1044, 1977.

178. Dasgupta, S. and Mitra, S. The role of DNA polymerase I-associated 5' exonuclease in replication of coliphage M13 replicative form DNA. Biochem. Biophys. Res. Commun. 78: 1108-1113, 1977.

179. Allison, D. P., Ganesan, A. T., Olson, A. C., Snyder, C. M. and Mitra, S. Electron microscopic studies of M13 bacteriophage DNA replication. J. Virol. 24: 673-684, 1977.

180. Dasgupta, S., Allison, D. P., Snyder, C. M. and Mitra, S. Base-unrepaired regions in supercoiled replicative form DNA of coliphage M13. J. Biol. Chem. 252: 5916-5923, 1977.

181. Mitra, S. and Stallions, D. R. The role of E. coli dnaA function and its integrative suppression in M13 coliphage DNA synthesis. Eur. J. Biochem. 67: 37-45, 1976.

182. Dasgupta, S. and Mitra, S. The role of E. coli dnaG function in coliphage M13 DNA synthesis. Eur. J. Biochem. 67: 47-51, 1976.

183. Dasgupta, R. and Mitra, S. Strand separation of DNA induced by ultra-violet irradiation in vitro. Biochim. Biophys. Acta 374: 145-158, 1975.

184. Mitra, S. and Stallions, D. R. Role of dna genes of Escherichia coli in M13 phage replication. Virology 52: 417-424, 1973.

185. Mitra, S. Inhibition of M13 phage synthesis by rifampicin in some rifampicin-resistant Escherichia coli-mutants. Virology 50: 422-430, 1972.

186. Dasgupta, R. and Mitra, S. Denaturation of DNA in vitro after X-irradiation. Biochem. Biophys. Res. Commun. 40: 793-799, 1970.

187. Datta, B. C. and Mitra, S. The release of 5'-nucleotidase and phosphodiesterase from Vibrio cholerae and Vibrio el tor. Indian J. Med. Res. 58: 1505-1509, 1970.

188. Roy, A. and Mitra, S. Increased fragility of E. coli after infection with bacteriophage M13. J. Virol. 6: 333-339, 1970.

189. Roy, A. and Mitra, S. Susceptibility of E. coli K12 to actinomycin D after infection with phage

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M13. Nature (U.K.) 228: 365-366, 1970.

190. Mitra, S. and Basu, S. 1968. Some biophysical properties of a vibrophage and its DNA. Biochim. Biophys. Acta 155: 143-149, 1968.

191. Mitra, S., Reichard, P., Inman, R., Bertsch, L. and Kornberg, A. Enzymatic synthesis of deoxyribonucleic acid. XXII. Replication of a circular single-stranded template. J. Mol. Biol. 24: 429-447, 1967.

192. Mitra, S. and Kornberg, A. Enzymatic mechanisms of DNA replication. Symp. on Macromol. Metabolism (New York) and J. Gen. Physiol. (Suppl.) 49 (Part 2): 59-79, 1966.

193. Mitra, S. and Kaesberg, P. Biophysical properties of RNA from turnip yellow mosaic virus. J. Mol. Biol. 14: 558-571, 1965.

194. Mitra, S., Enger, M. D. and Kaesberg, P. Physical and chemical properties of RNA from the bacterial virus R17. Proc. Natl. Acad. Sci. USA 50: 68-75, 1963.

195. Enger, M. D., Stubbs, E. A., Mitra, S. and Kaesberg, P. Biophysical characteristics of the RNA-containing bacterial virus R17. Proc. Natl. Acad. Sci. USA 49: 857-860, 1963.

196. Mitra, S. and Kaesberg, P. Interaction of polyamines with turnip yellow mosaic virus RNA. Biochem. Biophys. Res. Commun. 11: 146-151, 1963.

197. Chatterjee, G. C. and Mitra, S. Studies on phospholipid-splitting enzyme of Vibrio el tor. Biochem. J. (U.K.) 83: 384-387, 1962.

198. Mitra, S. and Chatterjee, G. C. Lecithin-splitting enzyme of Vibrio el tor. Nature (U.K.) 189: 837, 1961.

199. Mitra, S. DNA replication in viruses. Ann. Rev. Genet. 14: 347-397, 1980.

200. Snow, E. T. and Mitra, S. Is the incorporation of modified deoxynucleotide precursors during DNA replication a significant factor in mutagenesis? Cancer Invest. 5: 119-125, 1987.

201. Snow, E. T. and Mitra, S. Role of carcinogen-modified deoxynucleotide precursors in mutagenesis. Mutat. Res. 200: 157-164, 1988.

202. Mitra, S. and Kaina, B. Regulation of repair of alkylation damage in mammalian genomes. Prog. Nucl. Acids Res. Mol. Biol. 44, 109-141, 1993.

203. Mitra, S., Hazra, T. K., Roy, R., Ikeda, S., Biswas, T., Lock, J., Boldogh, I., and Izumi, T. Complexities of DNA base excision repair in mammalian cells. Molecules and Cells. 7:305-312, 1997

Other Publications:1. Mitra, S. Does evening sun increase the risk of skin cancer? Proc Natl Acad Sci U S A.

2011, 22; 108(47):18857-8. (Invited Commentary to the article of Gaddamedhi, S. et al.,PNAS 108:18790;2011)

Book Chapters:

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1. Mitra S, Hegde ML, Theriot CA, Das A, Hegde PM and Hazra TK. Complexity in repair of oxidative genome damage and its regulation. Proceedings of Princess Takamatsu Symposium, Tokyo, Japan, 2009.

2. Roy, R. and Mitra, S. “DNA Base Excision Repair: A Recipe for Survival”. In: The DNA Damage Response: Implications on Cancer Formation and Treatment. (K. K. Khanna and Y. Shiloh, Editors), Springer, New York, pages 179-208, 2009.

3. Boldogh, I., Bhakat, K., Bocangel, D., Das, G. C. and Mitra S. “Regulation of DNA repair and apoptosis by p53 and its impact on alkylating drug resistance of tumor cells.” In: DNA Repair in Cancer Therapy. Humana Press, New Jersey, pp. 73-108, 2004.

4. Bhakat, K. K., Yang S.H. and Mitra S. “Acetylation of human AP-endonuclease 1, a critical enzyme in DNA repair and transcription regulation.: In: Methods in Enzymology, RNA Polymerases and Associated Factors, Part D, Volume 371, 292-300, Sankar L. Adhya and Susan Garges, Eds., Elsevier Academic Press, San Diego, 2003.

5. Mitra, S., Hazra, T., and Izumi, T. “Nucleic Acid Synthesis”. In: Encyclopedia of Physical Science and Technology, Third Edition. Robert A. Myers (ed), Academic Press, San Diego, 2001.

6. Hazra, T. K., Hill, J. W., Izumi, T. and Mitra, S. “Multiple DNA Glycosylases for Repair of 8-oxoguanine and Their Potential In Vivo Functions”. In: Prog. Nucl. Acids Res. Mol. Biol., Base Excision Repair, Volume 68, (K. Moldave, S. Mitra, A. McCullough, R. S. Lloyd, S. H. Wilson, eds), Academic Press, San Diego, 2001.

7. Mitra, S., Izumi, T., Boldogh, I., Ramana, C. V., Hsieh, C. C., Saito, H., Lock, J. and Papaconstantinou, J. “Repair of oxidative DNA damage and aging: Central role of AP-endonuclease.” In NATO ASI Proceedings on Advances in DNA Damage and Repair: Oxygen Radical Effects, Cellular Protection, and Biological Consequences. ( M. Dizdaroglu and A. Karakaya, eds.) NATO ASI Series, Chapter 24, 295-311, Kluwer Academic/Plenum Publishers, New York, 1999.

8. Kaina, B., Haas, S., Groson, S., Grombacher, T., Dosch, J., Biswas, T., Boldogh, I., Mitra, S., and Fritz, G. Inducible responses and protective functions of mammalian cells upon exposure to UV light and ionizing radiation. In NATO ASI Proceedings on Fundamentals for the Assessment of Risks from Environmental Radiation: (G. Horneck, ed.) NATO ASI Series, 1999.

9. Hsie, A. W., Stankowski, L. F., Schenley, R. L., Foote, R. S., Mitra, S. and Thielman, H. W. An analysis of alkylating mutagenesis in CHO cells. In: Recent Trends in Medical Genetics (K. M. Marimuthu and P. M. Gopinath, eds.), Pergamon Press, Oxford, pp. 177 185, 1986.

10. Foote, R. S., Dunn, W. C., Lalley, P. A., Thompson, L. H. and Mitra, S. Regulation of 06-methylguanine-DNA methyltransferase in mammalian cells. In: Environmental Mutagenesis and Carcinogenesis (N. K. Notani and P. S. Chauhan, eds.), Bhabha Atomic Research Center, India, pp. 99 115, 1986.

11. Mitra, S., Sikpi, M. O., Preston, R. J. and Waters, L. C. Nonrandom distribution of mutations induced by X rays in a plasmid target in human cells. In: Multilevel Health Effects Research: From Molecules to Man (J. F. Park and R. A. Pelroy, eds.), Battelle Press, Richland, Washington, pp. 67-80, 1989.

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Abstracts: N/A

Presentations:

Invited Lectures at Symposia and Conferences:Invited lecture at Society of Toxicology Annual meeting, Phoenix, AZ (March,2014)Invited keynote lecture, US/EU Conference on DNA Repair, Taos NM, November, 2014Invited lecture at Peking Institute of Research, Beijing, China (October,2013)Invited talk, ISN meeting, Cancun, Mexico, 2013Keynote lecture, 15th Midwest DNA Repair meeting, Lexington, KY, 2013Invited talk, AT-Worskshop Delhi India, 2012Invited talk, EMGS meeting, Seattle, Washington, 2012Invited talk, US-EU conference on DNA Repair, Trondheim Norway, June 2012Invited speaker, 3rd Seeberg Symposium on DNA Repair, Trondheim, Norway (June, 2012)Invited speaker, EMS Annual Meeting, Bellevue, WA (September, 2012)Invited talk, Seeberg Symp, Oslo Norway, 2011Invited talk, INDICASAT, Panama, 2011Invited talk, US-India Conference on DNA Excision Repair, Hyderabad India, 2011Invited speaker, Indo-US Workshop on Base Excision Repair, Brain Function and Aging, Hyderabad, and

Silver Jubilee Meeting of the Soc. Neurochemists, India(January,2011)Invited speaker, 4th US-EU Conference on Base Damage and Repair, Oslo Norway (May, 2011)Invited speaker, Internat. Conference on Drug Discovery and Neurodegenerative Diseases, Panama (May, 2011). Invited talk, EMGS meeting, Dallas, 2010Distinguished Speaker, University of Florida, Gainesville (2010)Invited speaker, Annual EMS Meeting, Fort Worth, TX (October, 2010)Invited speaker and organizer, Genome Maintenance & stability Workshop, Niels Bohr Institute,

Copenhagen (Sept, 2010)Invited Speaker, 2nd Seeberg Symposium, Geiranger, Norway (2009)Invited Speaker, AACR Annual Conference, Denver (2009)Invited Speaker, ASM Conference on DNA Repair and Mutagenesis, Whistler, B. C., Canada (2009)Invited Speaker, Princess Takamatsu Symposium on Cancer and DNA Repair, Tokyo (2009)Invited Speaker, Princess Takamatsu Symposium, Japan, 2009Invited Speaker, Second International Conference on Frontiers in Biomedical and Environmental Health

Sciences: DNA Repair and Cancer Biology, Hangzhou China, (2008)Invited Speaker, 10th International Workshop on Radiation Damage to DNA, Urabandai, Japan (2008)Invited Lecture on “History of DNA Repair” at M.D. Anderson Cancer Center, Smithville and web lecture in

DNA Repair series (K. Kraemer, NIH) (2008)Distinguished “Speaker, Cancer Center, LSU School of Medicine, New Orleans (2008)Invited Speaker, Gordon Conference on Mammalian DNA Repair, Ventura (2007)Distinguished Speaker, Fox Chase Cancer Center, Philadelphia (2007)Invited Speaker, SBDR/EU DNA Repair Symposium, Berkeley (2007)Invited Speaker, Golden Jubilee Symposium of Indian Institute of Chemical Biology, Calcutta, India (2007)Distinguished Speaker, Toxicology Program, University of Kentucky, Lexington (2007)Invited Speaker, DNA Repair: from Molecular Mechanism to Human Disease, The Netherlands (2006)Invited Speaker, The Seeberg Symposium on DNA, Lofoten Islands, Norway (2006)

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Invited Speaker, Gordon Conference on Mammalian DNA Repair, Ventura (2005)Invited Speaker, Gordon Conference on Genetic Toxicology, New London (2005Invited Speaker, Keystone Symposium, Taos, New Mexico (2005)Keynote Address, MGMT Meeting, Keele Conference Park, UK (2005)Invited Speaker, International Conference on Environmental Mutagens (ICEM), San Francisco (2005)Invited Speaker, LVMH Recherche Mitochondria Symposium, Paris, (2005)Invited Speaker, 2nd US-EU Meeting on DNA Repair, Erice, Italy (2005) Session Chair and Speaker: Radiation Research Society Annual Meeting, Denver (2005)Invited Lecture, Emory University, Atlanta (2004)Speaker and Member of Organizing Committee, 8th International Workshop of Radiation Damage to DNA,

Banff (2004)Invited Lecture, ASM Conference on DNA Repair and Mutagenesis, Bermuda (2004)Invited Lecture, 2nd US-Japan DNA Repair Meeting, Honolulu (2004)Gordon Conference on Mammalian DNA Repair, Ventura (2003)Invited Lecture, National Cancer Institute (2003)Invited Speaker, Retreat of University of Texas-San Antonio Nathan Shock Aging Center (2003)Invited Speaker, 1st US/EU Conference on DNA Repair, Virginia (2003)Invited Speaker, DNA Repair Workshop, National Institute of Aging, Biology of Aging Program,

Washington, DC (2003)Invited Lecture, University of Pittsburgh (2002)Invited Lecture, American Health Foundation (2002)Chair, M. D. Anderson Basic Research Symposium (2002)Invited Lecture, Winter Gordon Conference, Mammalian DNA Repair (2001)Invited Lecture, University of Indiana, Combined Lecture Series in the Departments of Medicine and Pediatrics, Indianapolis (2001)Radiation Research Society Annual Meeting, San Juan, PR (2001)Invited Lecture, University of Puerto Rico, Department of Biochemistry (2001)Invited Lecture, German Society of Pharmacology & Toxicology Meeting, Mainz, Germany (2001)Invited Lecture, 3rd Annual Midwest DNA Repair Symposium, Indianapolis (2001)Invited Lecture, 4th Annual John B. Little Symposium, Boston (2001)Invited Lecture, Workshop on DNA Repair, Bethesda (2001)Invited Lecture, University of Texas Health Science Center, San Antonio, Institute of Biotechnology, San Antonio (2001)Invited Lecture, Texas A&M University (2000)Gordon Conference, Ventura, CA (1999)ASBMB Annual Meeting, San Francisco, CA (1999)FASEB Annual Meeting, Copper Mountain, CO (1999)Invited Lectures by the Ministry of Education Science Sports and Culture of Japan

(lectures to be given at different universities and cities in Japan) Hiroshima (1999)Invited Lecture at the Hungarian Academy of Science, Szeged, Hungary (1999)Japan-U.S. Exchange of Scientist Program, Traveling Lecturer (June, 1998)AACR Annual Meeting, New Orleans, LA (1998)12th Symposium of the Protein Society, San Diego, CA (1998)NIEHS Center of M.D. Anderson Cancer Center, Smithville (March, 1998)Radiation Research Society Annual Meeting Symposium (1997)NATO Advanced Study Institute, DNA Damage and Repair, Antalya Turkey (1997)International Institute of Anticancer Research, "Apoptosis", Athens, Greece (1997)Annual B. C. Guha Symposium on 'Recent Trends in Genetic Engineering and Molecular Science', Calcutta, India (1997)Indian Society of Clinical Biochemists Annual Meeting (December, 1997)Eppley Cancer Institute, University of Nebraska Medical Center (1997)University of South Alabama Medical Center, Mobile (1997)Invitation to the Special Commemorative Conference Celebrating 20th Anniversary of the Discovery on

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Oncogenes, Korean Society of Medical Biochemistry & Molecular Biology, Seoul, Korea (1996) Guha Research Conference, India (1996)Institute of Molecular Biology, Seoul National University, Seoul, Korea (1996)14th Annual Meeting of the Neurotrauma Society, Washington, DC (1996)University of Houston, Institute for Molecular Design (1995)Wells Center for Pediatric Research, Hematology/Oncology, Indiana University (1995)NCI-LSA Workshop on Secondary Leukemias, Rockville, MD (1995)Co-Convener, DNA Repair and Mutagenesis XVI International Biochemistry Congress (1994)University of Texas Medical Branch Faculty Research Colloquium (1993)Alfred Benzon Foundation Symposium, Copenhagen (1992)Winter Gordon Conference on DNA Repair, Oxnard, CA (1991)34th Annual Radiation Research Society Symposium, Tokyo, Japan (1991)Korean Society of Molecular Biology Symposium, Seoul, Korea (1991)15th International Cancer Congress, Hamburg (1990)Preuss Foundation Workshop, Boston, MA (1990)

Scientific Sessions as Convener

BER 2000 Workshop, San Luis Hotel, Galveston, TX March, 2000,

3rd US/EU Repair of Endogenous Genome Damage, Moody Gardens Hotel, Galveston, TX February, 2009,

Signature:

Date:

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curriculum vitae - pku · web viewcurriculum vitae sankar mitra, ph.d. date of preparation: october...

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