Curriculum Vitae Michele Cianci, Ph.D.

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Place and date of birth Treviso (Italy), 19th of December 1972 Citizenship: Italian Current address: Department of Agricultural, Food and Environmental Sciences

Università Politecnica delle Marche Via Brecce Bianche, 60131 Ancona, Italy

e-mail address m.cianci@univpm.it Web pages http://www.univpm.it/Entra/Engine/RAServePG.php/P/32071001041

9/idsel/738/docname/MICHELE%20CIANCI WoS Researcher ID http://www.researcherid.com/rid/I-5577-2012

CURRENT POSITION 2016/11 - present Assistant Professor, Department of Agricultural, Food and Environmental

Sciences, Marche Polytechnic University, Ancona (Italy) PREVIOUS POSITIONS 2006/09 - 2016/08 Project Leader - Faculty member at European Molecular Biology Laboratory

(EMBL) - Hamburg (DE). 2002/05 - 2006/08 Developmental Research Scientist at Science & Technologies Facilities

Council (STFC) - SRS Daresbury Laboratory (UK). EDUCATION 1999 - 2002

PhD in Crystallography, The Victoria University of Manchester, (United Kingdom), advisor Prof. J.R. Helliwell.

1992 - 1998 Degree in Chemistry at Universita’ degli studi di Padova (IT), advisors, Prof. M. Mammi and G. Zanotti.

AWARDS and HONORS 2014 Associate Professor (05/E1 - II Fascia - Biochemistry); Italian Ministry for

Education, University and Research (MIUR) 2008 Italian Crystallographic Association, AIC Nardelli Prize

2005

CCLRC (Science and Technology Facilities Council), UK, promotion to Senior Staff Scientist.

1999 Ph.D Scholarship, EPSRC (Engineering and Physical Sciences Research Council), UK

1999 Ph.D Scholarship, The University of Manchester, UK 1998 Chemistry professional society (IT); Italian Ministry for Education, University

and Research (MIUR) GRANT AWARDS and PARTICIPATION role 2007/01 - 2010/12 Project leader - DE/BMBF/05/ES7YE1, “Synchrotronstrahlfuehrungen fuer

strukturbiologische Forschung am PETRA III Speicherring”, EMBL Hamburg, Principal Investigator: Stefan Fiedler

2006/11 - 2008/03 Co-Applicant - BBSRC, BBE0019711, “Provision of the MAD/XAFS facility for the UK structural biology community”, STFC Laboratories, Principal Investigator: Hasnain S, Co-Applicants: Antonyuk S, Cianci M, Strange R.

2001/08 - 2006/08 Staff Scientist - UK/BBSRC/B15474, “NW Structure Genomics Centre's high-throughput MAD beamline for pathogen genomes”, STFC – Laboratories, Principal Investigator: Helliwell, JR and Hasnain S

Curriculum Vitae Michele Cianci, Ph.D.

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TEACHING, COURSES AND FACULTY ACTIVITIES 2016/present Biochimica 1 (ITA) - C.d.L. C.L.T. - Scienze e Tecnologie Alimentari (STAL),

54 hours, 6 CFU 2016/present Enzymology (EN) - C.d.L. C.L.M. - Food and Beverage Innovation and

Management (FABIAM), 54 Hours, 6 CFU 2016/present From experimental design to the writing of a scientific paper and research

evaluation (EN) – PhD course, 12 Hours 2008/2016 User and experiment supervision at MX beamlines at EMBL-DESY (DE). 2002/2006 User and experiment supervision at MX beamlines at Daresbury (UK). 2014/present Supervision of Dr. Abdul Samad (in collaboration with Dipl. Ing. Jiri Janata,

Laboratory for Biology of Secondary Metabolism, Prague, Czech Republic) 2014/2016 Supervision of Ph.D. student Luca Mazzei (in collaboration with Prof. Stefano

Ciurli, Univerisità di Bologna, Italy) 2011/2013 Supervision of Ph.D. student Ben Stauch (in collaboration with Dr. John

Overington, European Bioinformatics Institute (EBI), Cambridge, UK) 2004/2006 Supervision of Ph.D. student Helal Ahmed (joint-supervision with Prof. JR

Helliwell, Univeristy of Manchester, UK). 2004/2006 Supervision of Ph.D. student Nicola Pasquato (joint-supervision with Prof. G

Zanotti, Università di Padova, Italy)

PEER- REVIEWING 2003 - present. Referee for peer-reviewed journals: Acta Crystallographica Section D, Journal of

Synchrotron Radiation, Journal of Applied Crystallography, Federation of American Societies for Experimental Biology (FASEB) Journal, Genome Research, Structure

2007 - present Referee for funding agencies: Swiss National Science Foundation PROFESSIONAL SOCIETIES 2001 - present Italian and European Crystallographic Association (AIC, ECM) 2007 - present Societa’ Italiana Luce Sinchrotrone (SILS) 2006 - 2016 German Crystallographic Association (DKG) 1999 - 2006 British Crystallographic Association (BCA) CONFERENCE SCIENTIFIC COMMITTEE MEMBERSHIP

2015/01 Milan, Italy - EMBL Hamburg Dissemination Meeting - Integrated Facility for Structural Biology - M.Bolognesi, M. Cianci

2013 - 2010 Italy - XVIII to the XXI Meeting of the Italian Society for Synchrotron Radiation (SILS 2010 - 2013) - Turchini S., Cianci M., Mariani C., Balerna, M., Laberti, C., Cruciani, G., Paolasini, L.

2007

EMBL Hamburg, Germany - Beam line design workshop - ‘Macromolecular Crystallography@ Petra III’, organizers: T. Schneider, M. Cianci, G. Bourenkov

SELECTED CONFERENCE and WORKSHOP TALKS AND SEMINARS 2016/03 Stuttgart, (UK) - 24th Annual Meeting of the German Crystallographic Society

(DGK) “Sulfur SAD phasing of the multimeric anaphase-promoting complex Cdc23Nterm with a tailored X-ray beam size of 2.69 Å wavelength at EMBL P13 beamline at PETRA III@DESY”

2015/01 Nottingam (UK) - CCP4 Study week-end “EMBL P13 beamline at PETRA III @DESY: harnessing sulfur anomalous signal from biological macromolecules with a 4.6keV X-ray beam”

Curriculum Vitae Michele Cianci, Ph.D.

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2014/12 Trieste (Italy) - ICTP workshop - Advanced Workshop on Structural Biology: “Using Synchrotron Radiation to Visualise Biological Molecules – A Macromolecular crystallography experiment”

2014/02 Stuttgart (Germany) - CECAM workshop "Biomolecules under non-natural conditions" - “Analysis of counter-ion effects in nonaqueous enzymology of subtilisin”

2012/09 Hamburg (Germany) - 6th International Congress on Biocatalysis – “Analysis of counter-ion effects in nonaqueous enzymology of subtilisin”

2011/07 Hamburg (Germany) - Selected Biosciences Meeting- “Lessons of Diffraction Resolution and how the Structures of the Various Crustacyanins have been Solved”

2010/05 Hamburg, (Germany) - EMBO Conference Series Catalytic mechanisms by biological systems: at the interface between chemistry and biology, “Crystallographic Analysis of Counter-Ion Effects on Subtilisin Enzymatic Action in Acetonitrile”.

2009/09 Salerno (Italy) - Italian Crystallographic Association Congress. “Biocrystallography for human health”.

2008/09 Setri Levante (Italy) 1st SIMP - Italian Crystallographic Association Congress. Nardelli Prize Lecture.

2008/08 Osaka (Japan) International Union Crystallography, XXI Congress, MS10; "The Interdependence of Wavelength, Redundancy and Dose on a Sulfur SAD Experiment. ".

2007/08 Soleil, Paris (France), 3rd BioXAS Study Weekend "Metalloproteomics" Workshop. - Facilities for Complementing X-ray Macromolecular Crystallography: Present and Future.

2007/07 Hamburg (Germany), EMBO BioXAS Practical Course on metalloproteins and organism tissue, “Combined PX and polarized XANES on protein crystals”

PUBLICATION LIST Michele Cianci, Ph.D.

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M Cianci, G Bourenkov, G Pompidor, I Karpics, J Kallio, I Bento, M Roessle, F Cipriani, S Fielder, TR Schneider (2017). P13, the EMBL macromolecular crystallography beamline at the low-emittance PETRA III ring for high- and low-energy phasing with variable beam focusing. Journal of Synchrotron Radiation 24, 323-332. L Mazzei, M Cianci, F Musiani, G Lente, M Palombo, S Ciurli (2017). Inactivation of urease by catechol: Kinetics and struture. Journal of Inorganic Biochemistry, 166, 182-189. U Zander, M Cianci, N Foos, Catarina S Silva, L Mazzei, C Zubieta, A de Maria, M H Nanao (2016). Acta Crystallographica, Structural Biology Section D72, 1026-1035. L Mazzei, M Cianci, F Musiani, S Ciurli (2016). Inactivation of Urease by 1,4-Benzoquinone: Chemistry at the Protein Surface (2016). Dalton Transactions. 45, 5455 - 5459. M Cianci*, M Groves, D Barford, TR Schneider (2016). Data collection with a tailored X-ray beam size at 2.69 Å wavelength (4.6 keV): Sulfur SAD phasing of Cdc23Nterm. Acta Crystallographica, Structural Biology Section D72, 403-412. L Mazzei, M Cianci, S Benini, L Bertini, F Musiani, S Ciurli (2016). The inhibition of urease by sulfite: a kinetic and structural study. Journal of Inorganic Biochemistry, 154, 42-49. M Schürmann, R Meijers, TR Schneider, A Steinbüchel, M Cianci* (2015). 3-Sulfinopropionyl coenzyme A (3SP-CoA) desulfinase from Advenella mimigardefordensis DPN7T: crystal structure and function of a desulfinase with an acyl-CoA dehydrogenase fold. Acta Crystallographica, Structural Biology Section D71, 1360-1372. S Begum, M Cianci, B Durbeej, O Falklöf, A Hädener, J. R. Helliwell, M. Helliwell, A. Regan, I. Watt (2015). On the Origin and Variation of Colors in Lobster Carapace. Physical Chemistry Chemical Physics. 17, 16723-16732. B Stauch, SJ Fisher, M Cianci* (2015). Open and closed states of Candida antarctica Lipase B: protonation and the mechanism of interfacial activation. Journal of Lipid research. 56, 2348-2358. M Cianci, C Folli, F Zonta, P Florio, R Berni, G Zanotti (2015). Structural evidence for asymmetric ligand binding to transthyretin. Acta Crystallographica, Structural Biology Section D71, 1572-1592. J Wuerges, L Caputi, M Cianci, S Boivin, R Meijers, S Benini (2015). The crystal structure of Erwinia amylovora levansucrase provides a snapshot of the products of sucrose hydrolysis trapped into the active site. Journal of Structural Biology 191, 290-298. P Florio, C Folli, M Cianci, D Del Rio, G Zanotti, R Berni (2015). Transthyretin binding heterogeneity and anti-amyloidogenic activity of natural polyphenols and their metabolites. Journal of Biological Chemistry. 290, 29769-29780. S Benini, L Caputi, M Cianci (2014) Cloning, purification, crystallization and 1.57 Å resolution X-ray data analysis of AmsI, the tyrosine phosphatase controlling amylovoran biosynthesis in the plant pathogen Erwinia amylovora. Acta Crystallographica, Structural Biology Communications Section F70, 1693-1696. M Toccafondi, M Cianci, S Benini (2014). Expression, purification, crystallization and preliminary X-ray analysis of glucose-1-phosphate uridylyltransferase (GalU) from Erwinia amylovora. Acta Crystallographica, Structural Biology Communications Section F70, 1249-1250. M Cianci*, JR Helliwell, J Negroni and PJ Halling (2014). Extensive counter-ion interactions with subtilisin in aqueous medium. RSC Advances, 4, 36771–36776. S Benini, M Cianci, L Mazzei, S Ciurli (2014). Fluoride inhibition of Sporosarcina pasteurii urease: structure and thermodynamics. Journal of Biological Inorganic Chemistry, 19, 1243-61.

PUBLICATION LIST Michele Cianci, Ph.D.

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Ferrario, M.; Alesini, D.; Alessandroni, M., …, M Cianci. et al. (2014). IRIDE: Interdisciplinary research infrastructure based on dual electron linacs and lasers. Nucl. Instr. & Methods In Phys. Res. Sect. A, 740, 138-146. L Caputi, M Cianci, S Benini (2013). Cloning, expression, purification, crystallization and preliminary X-ray analysis of EaLsc, a levansucrase from Erwinia amylovora. Acta Crystallographica, Structural Biology Communications Section F69, 570-573. S Benini, P Kosikowska, M Cianci, L Mazzei, AG Vara, Ł Berlicki, S Ciurli (2013). The crystal structure of Sporosarcina pasteurii urease in a complex with citrate provides new hints for inhibitor design. Journal of Biological Inorganic Chemistry, 18, 391-399. F Siewert, J Buchheim, T Hoft, S Fiedler, G Bourenkov, M Cianci, R Signorato (2012). High angular resolution slope measuring deflectometry for the characterization of ultra-precise reflective x-ray optics. Measurement Science & Technology, 23, 074015. D Lousa, M Cianci, JR Helliwell, PJ Halling, AM Baptista, CM Soares (2012). Interaction of Counterions with Subtilisin in Acetonitrile: Insights from Molecular Dynamics Simulations. Journal of Physical Chemistry. B, 116, 5838-5848. SJ Fisher, MP Blakeley, M Cianci, S McSweeney, JR Helliwell (2012). Protonation-state determination in proteins using high-resolution X-ray crystallography: effects of resolution and completeness. Acta Crystallographica, Structural Biology Section D68, 800-809. M Ferrari, C Folli, E Pincolini, TS McClintock, M Rössle, R Berni and M Cianci* (2012). Molecular Basis of crustacean shell colours: structure and properties of H1 and H2 crustacyanins from lobster Homarus Americanus. Acta Crystallographica, Structural Biology Communications Section F68, 846-853. S Benini, M Cianci, S Ciurli (2011). Holo-Ni2+ Helicobacter pylori NikR is a symmetric tetramer containing four square-planar nickel-binding sites at physiological pH. Dalton Trans. 40, 7831–7833. M Cianci*, B Tomaszewski, JR Helliwell and PJ Halling (2010). Crystallographic Analysis of Counterion Effects on Subtilisin Enzymatic Action in Acetonitrile. J.Am.Chem.Soc. 132, 2293-2300. A Arcovito, C Ardiccioni, M Cianci, P D'Angelo, B Vallone, S Della Longa (2010). Polarized X-ray Absorption Near-Edge Structure Spectroscopy of Neuroglobin and Myoglobin Single Crystals. The Journal of Physical Chemistry B, 114, 13223-13231. G Wellenreuther, M Cianci, R Tucoulou, W Meyer-Klaucke, H Haase (2009). Zinc-rich macrophage vesicels store the metal in a complexed form. Biochem Biophys Res Commun. 380, 198-203. M Cianci*, JR Helliwell and A Suzuki (2008). The interdependence of wavelength, redundancy and dose in sulfur sad experiments. Acta Crystallographica, Structural Biology Section D64, 1196-1209. HU Ahmed, MP Blakeley, M Cianci, DWJ Cruickshank, JA Hubbard and JR Helliwell (2007). The determination of protonation states in proteins. Acta Crystallographica, Structural Biology Section D63, 906-922. A Arcovito, M Benfatto, M Cianci, SS Hasnain, K Nienhaus, GU Nienhaus, C Savino, RW Strange, B Vallone, S Della Longa (2007). X-ray structure analysis of a metalloprotein with enhanced active-site resolution using in situ x-ray absorption near edge structure spectroscopy. Proc. Natl. Acad. Sci. U. S. A. 104, 6211-6216. N Pasquato, R Berni, C Folli, S Folloni, M Cianci, S Pantano, JR Helliwell and G. Zanotti (2006). Crystal structure of peach Pru p 3, the prototypic member of the family of plant non-specific lipid transfer protein pan-allergens. Journal of Molecular Biology. 356, 684-694.

PUBLICATION LIST Michele Cianci, Ph.D.

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R Pedrazzani, I Alessandri, E Bontempi, F Cappitelli, M Cianci, E Pantos, L Toniolo, L.E. Depero. Study of sulphation of Candoglia marble by means of micro X-ray diffraction experiments (2006). Applied Physics A-Materials Science & Processing 83, 689-694. M Cianci, S Antonyuk, N Bliss, MW Bailey, SG Buffey, KC Cheung, JA Clarke, GE Derbyshire, MJ Ellis, MJ Enderby, AF Grant, MP Holbourn, D Laundy, C Nave, R Ryder, P Stephenson, JR Helliwell and SS Hasnain (2005). A high-throughput structural biology/proteomics beamline at the srs on a new multipole wiggler. Journal of Synchrotron Radiation 12, 442-452. M Cianci, JR Helliwell, M Helliwell, V Kaucic, NZ Logar, G Mali and NN Tusar (2005). Anomalous scattering in structural chemistry and biology. Crystallography Reviews 11, 245-335. MP Blakeley, M Cianci, JR Helliwell and PJ Rizkallah (2004) Synchrotron and neutron techniques in biological crystallography. Chemical Society Review 33, 548-57. M Cianci*, JR Helliwell, D Moorcroft, A Olczak, J Raftery and PJ Rizkallah (2004). The role of wavelength and source in the search for sulfur atom positions evaluated in two case studies: lysozyme at room temperature and cryo apo crustacyanin A1. Journal of Applied Crystallography 37, 555-564. J Habash, JR Helliwell, J Raftery, M Cianci, PJ Rizkallah, NE Chayen, GA Nneji and PF Zagalsky (2004). The structure and refinement of apocrustacyanin C2 to 1.3 Å resolution and the search for differences between this protein and the homologous apoproteins A1 and C1. Acta Crystallographica, Structural Biology Section D60, 493-498.

JR Helliwell, YP Nieh, G Habbash, PF Faulder, J Raftery, M Cianci, M Wulff and A Hadener (2002). Time-resolved and static-ensemble structural chemistry of hydroxymethylbilane synthase. Faraday Discussion. FD122. A Olczak, M Cianci, Q Hao, PJ Rizkallah, J Raftery and JR Helliwell (2003). Softer-SWAT (Softer-Single Wavelength Anomalous Technique): potential in high-throughput protein crystallography. Acta Crystallographica, Foundation and Advances Section A59, 327-334. NE Chayen, M Cianci, JG Grossmann, G Habbash, JR Helliwell, GA Nneji, J Raftery, PJ Rizkallah and PF Zagalsky (2003). Unravelling the structural chemistry of the colouration mechanism in lobster shell. Acta Crystallographica, Structural Biology Section D59, 2072-2082. M Cianci, PJ Rizkallah, A Olczak, J Raftery, NE Chayen, PF Zagalsky and JR Helliwell (2002). The molecular basis of the coloration mechanism in lobster shell: b-crustacyanin at 3.2 Å resolution. Proc. Natl. Acad. Sci. U. S. A. 99, 9795-9800. M Cianci, PJ Rizkallah, A Olczak, J Raftery, NE Chayen, PF Zagalsky and JR Helliwell (2001). The crystal structure of lobster apocrustacyanin A1 using softer X-rays. Acta Crystallographica, Structural Biology Section D57, 1219-1229. NE Chayen, M Cianci, A Olczak, J Raftery, PJ Rizkallah, PF Zagalsky and JR Helliwell (2000). Apocrustacyanin A1 from the Lobster Carotenoprotein, a-crustacyanin; crystallisation and initial X-ray analysis involving softer X-rays. Acta Crystallographica, Structural Biology Section D56, 1064-1066. M Cianci, F Gliubich, G Zanotti and R Berni (2000). Specific interaction of lipoate at the active site of Rhodanese. Biochimica Biophysica Acta - Protein Structure and Molecular Enzymology 1481, 103-108. RJ Trevino, F Gliubich, R Berni, M Cianci, JM Chirgwin, G Zanotti, PM Horowitz (1999). NH2-terminal sequence truncation decreases the stability of bovine rhodanese, minimally perturbs its crystal structure, and enhances interaction with GroEL under native conditions. Journal of Biological Chemistry 274, 13938-47.

PUBLICATION LIST Michele Cianci, Ph.D.

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Book chapters M Cianci, JR Helliwell, M Helliwell (2012). Structural Diffraction Methods. In: Guagliardi A, Masciocchi N. Crystallography for Health and Biosciences. p. 205-223, VARESE:Insubria University Press, ISBN:9788895362489.

Journal Covers

March 2016, Acta Crystallographica Section D for the article " Data collection with a tailored X-ray beam size at 2.69 Å wavelength (4.6 keV): Sulfur SAD phasing of Cdc23Nterm”.

July 2015, Physical Chemistry Chemical Physics for the article "On the Origin and Variation of Colors in Lobster Carapace".

December 2008, Acta Crystallographica Section D for the article "Interdependence of wavelength, redundancy and dose in sulfur sad experiments".