Curriculum Vitae JUAN LOPEZ GARRIGA

Education Interests: Implementation of a coherent links between graduate and undergraduate research and educational curriculum and cultures to enhance university students' didactic multiplicity and knowledge. Support and establish pre-college education and university interactions (K-20+ Outreach University Programs), through, for example, Science Shows, the Global Learning and Observation in Benefit of the Environment (GLOBE) and the Graduate and Undergraduate Students Enhancing Science and Technology (STEM) in K-12 Schools (GUEST K-12) programs, that help to prepare the next generations of university students and professionals.

Research Interests: Structure, dynamics, and function relationships in hemeproteins involved in ligand transfer (hemoglobins), electron transfer (cytochromes), and ligand reactivity. For example, using site directed mutagenesis we tailor the structure of unique hemoglobins while FTIR, resonance Raman, vibrational analysis, and NMR spectroscopies are used to establish factor that define and modify these hemeproteins chemical structures. Time-resolved (pump and probe) absorption, IR, and rR techniques are used to determine the kinetic behavior of the reaction between hemoglobin and ligand (i.e. O2, CO, NO, and H2S) for the development of biosensors. Similarly, chemical dynamics in the picoseconds and femtoseconds time regime are used to further understanding the basis for sulfur reactivity with hemeproteins and its link to biological function.

Administrative Interest: Contribute to the vision and mission of the University of Puerto Rico at Mayagüez by promoting human learning, change, and societal events coherently in phase with the sciences, arts, technology, and engineering of the XXI century. Exercise the decision making process of consensus toward the projection and transformation of the University. Facilitate activities that can unerringly enhance knowledge, inquiry, and creativity of the members of the university community which restructure critical thinking and lead to representations of the dynamical sense of the world.

Present Positions Acting Dean of the College of Arts and Sciences UPR-Mayaguez. March 2010- Member of the Puerto Rico Academy of Arts and Sciences October 2005- Committee member to develop policies, mechanisms, and strategies for research In Biomedical Science in Puerto Rico, Project BRIN-INBRE NIH January 2000- Professor, University of Puerto Rico Mayagüez Campus, July 1997- Puerto Rico Global Learning Observations to Benefit Environment,GLOBE Director May 1996- Science on Wheels Program Director. September 1991- UPRM Chemistry Department, Graduate Committee. August 1989- Phone: (787) 265-5453 Email: juan.lopez16@upr.edu Fax: (787) 265-5453 sonw@caribe.net

Education Postdoctoral, Los Alamos National Laboratory. Mentor: Dr. W. Woodruff. July 1988-1989 Postdoctoral, Department Chemistry, Michigan State Univ. Mentor, Prof. G.T. Babcock Sept. 1986-July 1988 Ph.D. Physical Chemistry, Michigan State Uni. Mentor Prof. G.T. Babcock Sept. 1981- Aug. 1986 M.S. Chemistry, University of Puerto Rico-Mayagüez, Mayagüez P.R. May 1980 B.S. Chemical Engineering, UPR- Mayagüez, Mayagüez P.R. December 1975 H.S. Maria Cadilla High School, Arecibo Puerto Rico. May 1970

Honors & Awards ACS Ambassador in dissemination of Chemistry, 34th Senior Technical Meeting, Mayagüez P.R. Nov. 2010 Annual GK-12 program-NSF, Media Award, Washington, D.C. March 2009 ACS National Chemluminary Award “Outstanding Continuing Public Relations Program of a Local Section”, Boston, MA August 2007. Dedication of the 3th Biotechnology Week in Puerto Rico September 2006. Puerto Rico INDUNIV Research Consortium Recognition to the Science on Wheel Program September 2006. Member of the Puerto Rico Academy of Arts and Sciences October 2005. Recognition Award by Hostos High School, GLOBE Program May 2005. Recognition Award by the Department of Education of Puerto Rico, Office of Corporative Alliances

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Pro-Education. December 2004. Recognition Award by Graduate Students Chemistry UPR at Mayagüez . November 2004 Industrial and Educational Chemical Association of Puerto Rico Excellence Trajectory Award. July 2004. ACS-PR Award in Chemical Education (ACS-Puerto Rico Section). November 2003. Industrial Biotechnology, Distinguish Professor, UPR-Mayagüez.November 2003, 2004, 2005 Alumni Distinguish Professor, University of Puerto Rico at Mayagüez. September 2001. 2000 USA Presidential Award for Excellence in Science, Mathematics, Engineering Mentoring (PAESMEM). March 2000. Igaravídez Award-ACS in Research and Public Service (ACS-Puerto Rico).November 2000 University of Puerto Rico, Office of the President Science Award, October 2000 University of Puerto Rico Mayagüez Scholar Award. June 1997, 1998 EPSCoR-UPR Scholarly Productivity Award. February 2001,1999, 1996, 1994, 1992, 1990 Camille and Henry Dreyfus Teacher-Scholar Award, 1994 Provost Affirmative Action Postdoctoral Michigan State University, 1987 Monsanto Industrial Fellow, Michigan State University, 1986 Chemistry Outstanding Teaching Assistant, Michigan State University, 1984 Regional High School Science Fair Award, Maria Cadilla, Puerto Rico. 1970

Languages Catalonian, Spanish, and English

Previous Positions Acting Director of the UPR-Mayaguez Research and Development Center May 2010- July 2010 Associate Dean of Research College of Arts and Ciences UPR-Mayaguez. December 2009- March 2010 AD HOC Committee member to develop policies, mechanisms, and strategies for research and graduate school at the University of Puerto Rico at Mayagüez. January 2008- December 2009 ACS-Puerto Rico, Local Section Alternate Councilor January 2008-December 2009 ACS-Puerto Rico Local Section President . January 2005- December 2006 UPRM Research and Development Center, Member Board of Directors. Sept 2003-2006 ACS-Puerto Rico Local Section President. January 2005- December 2005 IUPAC Education Committee. January 2002-2004 ACS Student Chapter Co-Advisor. August 2002-2004 Director Center for Protein Research of the UPR-NIH-COBRE. August 2001- 2003 Academic Senator, UPR- Mayagüez Campus. September 1999- January 2002 Academic Affair Senate Committee. September 1999- February 2002 Chemistry Department Strategic Plan Committee. August 1995- January 1998 Associate Professor, Department of Chemistry UPRM. August 1992 -June 1996 ACS Southwest Chairperson. September 1995- 1996 Liaison Council of Undergraduate Research. October 1994-1996 Co-Director EPSCoR-DOE UPR-Mayagüez program. October 1994-1996 SEED program of the American Chemical Society. May 1990-1996

Assistant Professor, Department of Chemistry, UPRM. July 1989-1992 Research Associate, Chemistry, UPR-Mayagüez Campus. August 1980 - July 1981 Instructor of General Chemistry, UPR-Arecibo P.R. January 1976 - August 1977 Academic Monsanto Research Industrial Fellow (MSU) Summer 1986 Positions Research Assistant, (MSU) August 1983 - 1986 During Teaching Assistant, (MSU) August 1981 - 1983 Education Teaching Assistant, (UPR) August 1977 - 1980

Memberships. American Chemical Society, American Association for the Advancement of Science, Inter-American Photochemical Society, National Science Teachers Association

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UNIVERSITY SERVICE AND EDUCATION: Outreach Programs and Public service Science on Wheels (1990-present). Development of an outreach K-12 program " Science On Wheels" which consist from nine to fifteen Science Shows per semester. The number of Science shows, in cooperation with the teamwork of 136 university students (26 graduate and 101 undergraduates) has been leading 328 science shows presentations in school, Science parks, shopping center (like Plaza las Americas), Public places (like Paseo de la Princesa) to more than 100,000 students, teachers and general public. Television programs presented like Jacobo y el Arte, Entrevistando a Nuestros Representantes, television news, and radio interviews helped the Science on Wheels program to be present in all Puerto Rico. There is an international component of the program at the Parque de las Ciencias in Granada, Spain. Detailed information about the program can be obtained from http://sonw.uprm.edu. Teachers Training in Science and Technology. GLOBE (Global Learning and Observations to Benefit the Environment) Program, CBL (Calculator bases laboratory), SMI and (scientific method and inquiry) from 1997 to present, 350 schools participated on the GLOBE workshops. The number of activities in cooperation with the teamwork of 167 university students (114 graduate and 53 undergraduates) lead to the training of 609 teachers in one week long 22 workshops,and 1,337 teachers in one day 70 workshops, and 1,371 direct visits to schools. The direct impact have been training of near 25,000 students pre-college students in science and technology.

UNIVERSITY RESEARCH INTEGRATED TO EDUCATION: Training, Publications, and Funding Total number of Peer Review Publications: 68 Publications in process: 2 Invited Seminars Presentations: 103 (32 National and International and 71 Local Puerto Rico Presentations in Technical Meetings: 102 (60 National-International and 42 Local Puerto Rico) Active Research Proposals, October 2004 to April 2013: 4, total funding ~ $1,668,305 Active K-12 Educational Proposals, March 2004 to March 2013: 4, total funding ~ $480,000 Active Training Undergraduate –Graduate Educational Proposals: August 2010- July 2014 $5,130,133 Research proposal Granted: 23, November 1989 – present. Total funding ~ 16,380,253 K-12 Educational Proposals Granted: 17, Nov.1989 – present . Total funding ~ $ 7,480,721 Total funding for all programs from May 1989 – present ~ $ 28,991,107

Graduate and Undergraduate Student Training (1989 - present ) Number of graduate students who successfully finished the PhD degree: 4 Number of graduate students who successfully finished the MS degree: 46 Number graduate student send to collaborating International Laboratories (Spain, France, Switzerland): 12 Number of undergraduate students trained in the laboratory: ~240 Actual number of PhD graduate students in the laboratory: 10 Actual number of undergraduate students in the laboratory: 12 Graduate Students Presentations in Technical Meetings: 233 (80 National/ International and 153 Local) Undergraduate Students Presentations in Technical Meetings: 101 (14 National/International and 87 Local) High Schools Students Presentations in Technical Meetings: 20 (9 National/International and 11 Local) Number of graduate university students trained to work with K-12 students and teachers: 167 Number of undergraduate students trained to work with K-12 students and teachers: 127

Education and Course Development. Teaching undergraduate and graduate courses in inorganic chemistry, physical chemistry, biophysics, and biospectroscopy. Integrated Inorganic Laboratory, undergraduate (in collaboration with Dr. Mayra Cadiz) 1990

Physical Chemistry for Non-Majors (in collaboration with Dr. Ismael Scott) 1991 Bio-inorganic Chemistry, Graduate and undergraduate (Special Topics) 1992 Bio-spectroscopy Techniques 1998

Professional Development International School on Biological Crystallization 2006 Certification of GLOBE Teachers Trainer of Trainees. 2000

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School in Ultrafast Dynamical Processes in Condense Phase 1996

Conferences and Meetings Organization 1. Center for Protein Research of the University of Puerto Rico. Summer seminar series in Protein Structure, Function and Dynamics. San Juan. August 2003 2. Center for Protein Research of the University of Puerto Rico “First Colloquium in Protein Structure, Function and Dynamics: Frontiers in Protein Spectroscopy” Ponce, Feb. 2004 3. Center for Protein Research of the University of Puerto Rico “Second Colloquium in Protein Structure, Function and Dynamics, San Juan Feb. 2005 4. Local Section 29th ACS Senior Meet. La Pargera, Lajas P.R November 2005 5. Local Section 41st ACS Junior Technical Meeting, UPR Cayey, March 2006 6. Local Section 30th ACS Senior Meet. Dorado. P.R November 2006 7. Annual GK-12 conference-NSF,Washington, D.C. March 2009 8. International crystallization competition high school students Puerto Rico-Spain May 2009

Agency Reviewed Proposals

1. National Science Foundation 2. Philip Morris External Research Program 3. Resource Center University of Puerto Rico at San Juan 4. Development and Research Center, University of Puerto Rico at Mayagüez 5. Pre-college Science fair referee

Reviewer of Publications for: American Chemical Society (Journal of American Chemical Society, Biochemistry), Elsevier, and Springer.

Research Publications (total 68) 1. "The Raman Spectra of Selenomethionine and Selenocystine" J. Lopez-Garriga, T.C. Jao and W.E. Rudzinkski, J. Inorg. Biochem. 1981, 14, 177-188. 2. "Factors Influencing the C=N Stretching Force Constant in Neutral and Protonated Schiff's Bases" J.J. López-Garriga, G.T. Babcock and J.F. Harrison, Biophysical J. 1985, 96. 3. "Increase in the C=N Stretching Frequency Upon Complexation of Trans-Retinylidene n-butylamine with General Lewis Acid", J.J. López-Garriga, G.T. Babcock and J.F. Harrison, J. Am. Chem. Soc., 1986, 108, 7131-7133. 4. "Factors Influencing the C=N Stretching Frequency in Neutral and Protonated Schiff's Bases" J.J. López-Garriga, G.T. Babcock and J.F. Harrison, J. Am. Chem. Soc., 1986, 108, 7241-7251. Increase in the C=N Stretching Frequency Upon Complexation of Trans-Retinylidene 5. n-butylamine and Other Schiff's Bases with General Lewis Acid",J.J. López-Garriga, G.T. Babcock and J.F. Harrison, Proceedings X on Raman Spectroscopy, (W.L. Peticolas,B. Hudson, ed) University of Oregon Press, pp. 1434 - 1435, 1986. 6. "Rehybridization of the C=N Bond Upon Protonation of Methylimine Increases the C=N Stretching Force Constant". J.J. López-Garriga, G.T. Babcock and J.F. Harrison, J. Am. Chem. Soc., 1986, 108, 7251-7254. 7. "Factors Influencing the C=N Stretching Frequency in Neutral and Protonated Schiff's Bases" J.J. López-Garriga, Ph.D. Thesis, 1986 Michigan State University. 8. Metal-Ligand Vibrations of Cyanoferric Myeloperoxidase and Cyanoferric Horseradish Peroxidase: Evidence for a Constrained Heme Pocket for Myeloperoxidase" J.J. López-Garriga, W.A. Oertling, R.T. Kean, H. Hoogland,R. Wever, and G.T. Babcock, Proceedings XI Raman Spectroscopy, London, U.K., 980-982, 1988. 9. "Time-Resolved Infrared Observation of a CuB+-CO Phototransient in Bovine Cytochrome Oxidase at 300 K.", R.B. Dyer, O. Einarsdóttir, P.M. Killough, J.J. López-Garriga and W.H. Woodruff. J. Am. Chem. Soc. 1989, 111, 7657-7659.

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10. "The Orientation of CO in Carbonmonoxy Cytochrome Oxidase and Its Transient Photoproducts. Direct Evidence from Time-Resolved Infrared Linear Dichroism" R.B. Dyer, J.J. López-Garriga, O. Einarsdóttir, and W.H. Woodruff. J. Am. Chem. Soc. 1989, 111, 8962-8963 11. "Metal-Ligand Vibrations of Cyanoferric Myeloperoxidase and Cyanoferric Horseradish Peroxidase: Evidence for a Constrained Heme Pocket for Myeloperoxidase" J.J. López-Garriga, W.A. Oertling, R.T. Kean, H. Hoogland, R. Wever, and G.T. Babcock, Biochemistry, 1990, 29, 9387-9395. 12. "Resonance Raman Characterization of Free-Base Porphycene and Ni(II), Co(II), Fe(II) and Fe(III) Porphycene Complexes". W.A. Oertling, W. Wu, J.J. López-Garriga, Y. Kim, C.K. Chang, J. Am. Chem. Soc., 1991, 113, 127-134. 13. "Photodissociation and recombination of carbonmonoxy cytochrome oxidase: Dynamics from picoseconds to kiloseconds" O. Einarsdóttir, R.B. Dyer, D.D. Lemon, P.M. Killough, S.M. Hubig, J.J. López-Garriga, G. Palmer and W.H. Woodruff. Biochemistry , 1993, 32, 12013-24. 14.Spectroscopic Evidence for the Rehybridization mechanism Upon Protonation of Retinal Schiff's Bases" J. López-Garriga and J. Lastra-Hernandez. Proceedings XIV Raman Spectroscopy (N.-T Yu and X.-Y. Li Editors) J. Wiley & Sons Ltd. pp 98-99, 1994. 15. J.Colon, J. Lopez-Garriga, A. Navarro, M. Maldonado, Characterization of Ligand Binding and Electron Transfer Properties HbI from L. pectinataJ. Inor. Biochem. 1995, 59, 447. 16. J. Colon, A. Navarro, M. Maldonado. Structure and Dynamic Behavior of Heme-CO of Hemoglobin I from Lucina pectinata" J. López -Garriga, Spectroscopy of Biological Molecules (J.C. Merlin, S.Turrell, J.P. Huvenne Ed) Kluwer Acad. Pub. Boston. pp 233-234, 1995 17. Resonance Raman Studies of AquoHbI from Lucina pectinata@ Y. Echevarria, , J. Cerda, J. Colon , J. López -Garriga. Proceedings XIV Raman Spectroscopy (S.A. Asher and P.B. Stein Editors) J. Wiley & Sons Ltd. pp 438-439, 1996 18. Photocatalyst of VHOC using a TiO2 Photosensitized by Phthalocyanines. J. Cajicas, J. López -Garriga. 212th ACS National Meeting, Orlando, Florida, Vol 32(2), 36-37. 1996 19. A. Navarro, J. Gonzalez-Lagoa, R. Lopez, J. Lopez-Garriga J. Colon, Control of CO Binding States and Dynamics in HbI of Lucina pectinata by Aromatic Residues. Inorg. Chim. Acta. 1996, 243, 161-166. 20. Time-Resolved Vibrational Spectroscopy P. Ford, J.Harris, J. Lopez-Garriga, J.I. Zink. Inter-American Photochemical Society, 1996, 19, 20-23. 21. Determination of VHOC including Fluoro Derivatives from Tropical Natural Environments. D. Acosta, I. Vazquez, B. Cruz, J. López -Garriga. 212th ACS National Meeting, Orlando, Florida, Vol 32(2), 63-64. 1996 22. New Record of Extremely Halophilic Archaebacteria for Puerto Rico and the Caribbean R. Montalvo, A. Ruiz, J. Lopez-Garriga, Caribbean J. Science 1997, 33, 98-104. 23. Science on Wheels: A Coherent Link Between Educational Cultures. J. Lopez-Garriga, Y. Echevarria, Y. Muñoz, V. Torres, J. Chem. Ed. 1997, 74, 1346-1349. 24. Structural characterization and dynamics events in hemoglobin I from Lucina pectinata: Unusual conformation of propionates and vinyl's heme peripheral groups. E. Silfa, M. Almeida, J. Cerda, S. Wu, J. López -Garriga, Spectrosc. Biol. Mol.: Trends, 79-80, 1997 25. Halogeometricum borinquense gen. nov., sp. Nov., a Novel Halophilic Archaeon from Puerto Rico. R. Montalvo, R.H., Vreeland, A. Oren., M. Kessel, C. Betancourt, J. López-Garriga Inter. J. Syst. Bacteriol. 1998, 48, 1305-1312. 26. Resonance Raman of Heme-Ligand Complexes of HbI from L. pectinata. E. Morales, J. López-Garriga. Proceedings XVI Raman Spectroscopy ( A.M. Heyns Editor) J. Wiley & Sons Ltd. pp, 294-295, 1998 27. Orientation of the Heme Vinyl Groups in the H2S-Binding HbI from Lucina pectinata. E. Silfa, M. Almeida, J. Cerda, S. Wu, J. López-Garriga Biospectroscopy 1998, 4, 311-326. 28. Unusual Flexibility of the Heme Propionates in the Hydrogen Sulfide-Binding HbI of L. pectinata. J. F. Cerda, E. Silfa J. López-Garriga. J. Am. Chem. Soc. 1998, 120, 9312 - 9317. 29. Halogeometricum borinquense, 16S rRNA sequence , R. Montalvo, R.H., Vreeland, A. Oren., M. Kessel, C. Betancourt, J. López-Garriga. Genbank number AF002984, 1998. 30. Resonance Raman Studies of the Heme-Ligand Active Site of HbI from Lucina pectinata. J. Cerda, Y. Echevarria, J. López -Garriga. Biospectroscopy 1999, 5, 289-301 31. The cDNA-Derived Amino Acid sequence of HbI from Lucina pectinata. F.M. Antommattei, T. Rosado, C.L. Cadilla, J. López-Garriga. J. Protein. Chem. 1999, 18, 831-836.

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32. Natrinema thermotolerans sp. nov., a halophilic archaeon from Puerto Rico. A. Oren, J. López -Garriga, R. Montalvo, R. Vreeland, A. Ventosa, M. Kamekura, Inter. J. Syst. and Evol. Microbiol. 2000, 50, 1065-1071. 33. Lucina pectinata full-length cDNA sequence of hemoglobin I.M. Antommattei, T. Rosado, C.L. Cadilla, J. López-Garriga. , Genbank number AF187049F, 1999 34. Hands-on Chemistry Demonstrations and the Global Learning and Observations to Benefit the Environment (GLOBE) Program. R. Camacho, J. López-Garriga, J. Chem. Ed., 2000, 77, 1586-1589. 35. Natrinema thermotolerans 16S rRNA sequence, Oren, J. López-Garriga, R. Montalvo, R. H. Vreeland, A. Ventosa, M. Kamekura Genbank number AF115478, 2000. 36. W. De Jesus, J. Cortes, J. López-Garriga. Formation of Compounds I and Compound II Ferryl Species in the Reactions of Hemoglobin I from Lucina pectinata with Hydrogen Peroxide. Arch. Biochem. Biophys., 2001, 390, 304-308 37. T. Rosado, F. Antommattei, C. Cadilla, J. López-Garriga. Expression and Purification of Recombinant Hemoglobin I from Lucina pectinata. J. Protein. Chem. 2001, 20, 311-31 38. W. De Jesús-Bonilla, E. Ramírez-Meléndez, J. Cerda, J. López-Garriga. Evidence for a Nonhydrogen Bonded Compound II in the Cyclic Reaction of Hemoglobin I from Lucina pectinata with Hydrogen Peroxide Biopolymers (Biospectroscopy) 2002, 62, 176-185. 39. Ramos C, Ramírez E, Ruiz C, Pietri R, Helbing J, Chergui M, López Garriga J Investigation of the Carbonmoxide and Oxy Hemoglobin I Complexes from Lucina pectinata in Femtosecond Transient Absorption. Femtochemistry and Femtobiology (World Scientific Publishing House, Douhal and Santamaria Ed.) 2002, Pag. 720-730. 40. E. Torres C.L. Cadilla, J. López-Garriga. Lucina pectinata, full-length cDNA sequence of hemoglobin II Genbank number (2003) AA089499.1. 41. E. Torres, J. Y. Renta, Y. Rodríguez, J. López-Garriga, C. L. Cadilla. The cDNA-Derived Amino Acid Sequence of Hemoglobin II from Lucina Pectinata. Journal of Protein Chemistry, 2003, 22, 683-690. 42. E. Collazo, R. Pietri, W. De Jesús, C. Ramos, A. Del Toro, R., León, C., Cadilla, J. López-Garriga. Functional Characterization of the Purified Holo form of Hemoglobin I from Lucina pectinata Over-Expressed in Escherichia coli. The Protein Journal. 2004, 23, 239-245. 43. E. Ramirez, C. Ramos, M. Rodríguez, R. Pietri, M. Chergui, J.López-Garriga. Ultrafast Studies of Ferric Complexes of Hemoglobin I from Lucina pectinata. Femtochemistry and Femtobiology 2004 (Ed. M. M. Martin, J. T. Hynes) Elsevier N.Y pag. 395-398. 44. Helbing, J., Bredenbeck, J., Hamm, P. Bonacina, L., van Mouririk, F., Chaussard, F., Gonzalez, A., Chergui, M., Pietri, R., Ramos, C., Ruiz, C., López-Garriga, J. Time-resolved visible and infrared study of the cyano complexes of Myoglobin and Hemoglobin I from Lucina pectinata Biophysical Journal, 2004, 87, 1-11. 45. Ruth Gretchen León, Hélène Munier-Lehmann, Octavian Barzu, Véronique Baudin Creuza, Ruth Pietri, Juan López-Garriga and Carmen L. Cadilla. High-level production of recombinant wild type and mutant sulfide-reactive hemoglobin I from L. pectinata in E. coli. Protein Expression and Purification, 2004, 38, 184-195. 46. Saliceti-Piazza, L., Buxeda, R., López -Garriga, J. Transformation of the Teaching Methodology in a Laboratory Course of the Industrial Biotechnology Curriculum. World Innovations in Engineering Education and Research. 2004, Chapter XX. 47. Ruth Pietri, Laura Granell, Anthony Cruz, Walleska De Jesús, Ariel Lewis, Ruth León, Carmen Cadilla, Juan López Garriga. Tyrosine B10 and Heme-ligand Interactions of Lucina pectinata Hemeglobin II: Control of Heme Reactivity. Biochimica et Biophysica Acta - Proteins and Proteomics 2005, 1747, 195-203. 48. Ruth Pietri, Laura B. Granell, Ruth León, Carmen Cadilla, J.López-Garriga. Hemoglobin I from Lucina pectinata: A model for distal heme-ligand control. Biochimica et Biophysica Acta - Proteins and Proteomics 2006, 1764, 758-765. 49. Gavira, J. A., Camara-Artigas, A., De Jesus, W., López-Garriga., J. , Garcia-Ruiz, J.M. Capillary Crystallization and molecular replacement solution of Hemoglobin II of the Clam Lucina Pectinata. Acta Crystallograph Sect F Struct Biol Cryst Commun, 2006, 62, 196-9. 50. Walleska De Jesús-Bonilla1, Anthony Cruz, Ariel Lewis, José Cerda, Daniel E. Bacelo, Carmen L. Cadilla

, Juan López-Garriga. Hydrogen bonding conformations of Tyrosine B10 tailor the hemeprotein reactivity of ferryl species. J. Biol. Inorg. Chem. 2006, 11, 334-342. 51. Fernandez-Alberti, S., Bacelo, D.E., Binning, R.C., Echave, J., Chergui, M., Lopez-Garriga, J. Sulfide-binding hemoglobins: Effects of mutations on the dynamics. Biophysical J. 2006, 91, 1698-1709.

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52. Gavira, J.A., Camara. A., de Jesús, W., López-Garriga, J., Garcia-Ruiz, J.M. Structure and ligand selection of hemoglobin II from Lucina pectinata. RCSB PDB code 2OLP (2007) 53. Lopez-Garriga, J., Mercado, S., Camacho, R. Expository programs: An effective approach to enhance K-12 engineering education. ICEE, Chapter 6 (Involving Engineering Schools/Universities Technology and Science School Outreach) 2007, 1- 6. 54. Rivera,L.E., Lopez-Garriga,J. and Cadilla,C.L. Lucina pectinata full-length cDNA sequence of hemoglobin III. Genbank number ABS87592, accession EU040120.1 (2007) 55. De Jesús-Bonilla, W., Yiping Jia, Y., Alayash, A.I., López Garriga, J. Heme pocket geometry of Lucina pectinata hemoglobin II restricts nitric oxide and peroxide entry: A model of ligand control for the design of a stable oxygen carrier. Biochemistry, 2007, 46, 10451-10460. 56. Angelines Castro, D. Jimenez, J. López Garriga, M. Torres. Immobilization of myoglobin from horse skeletal muscle in hydrophilic polymer networks. Journal of Applied Polymer Science, 2008, 107(2), 881-890. 57. Rivera, L. Lopez-Garriga, J., Cadilla, C.L. Characterization of the full length mRNA coding L. pectinata HbIII revealed an alternative polyadenylation site. Gene 2008, 410, 122-128 58. Gavira, J., Camara-Artigas, A., De Jesus, W., Pietri, R, Lewis, A., Yeh, S-R, López-Garriga, García-Ruiz, J. M. Structure and ligand selection of hemoglobin II from L. pectinata J. Biol. Chem. 2008, 283, 9414-9423. 59. Ramirez, E., Cruz, A., Rodriguez, D., Uchima,L., Pietri, R., Santana, A., Lopez-Garriga, J., Lopez, G.E. Effects of active site mutations in Hemoglobin I from Lucina pectinata: A molecular dynamic study. Molecular Simulations. 2008, 34, 715-725. 60. Carlos R. Ruiz-Martínez, Carlos A. Nieves, Rafael A. Estremera, José A. Gavira, Luis A. González-Ramírez, Juan J. López-Garriga and Juan M. García-Ruiz. Crystallization and Diffraction Patterns of the Oxy and Cyano Forms from the Lucina pectinata Hemoglobins Complex. Acta Crystallograph Sect F Struct Biol Cryst Commun. 2009, F65, 25-28. 61. R., Pietri, León, R.G., Kiger, L., Marden, M. C., Bacelo, D., Binning Jr. R. Cadilla, C. L., López-Garriga, J. Ligand-binding control in HemoglobinI from L. pectinata: Structural implications of B10, E7 and E11 mutants. Biochemistry 2009, 48, 4881-4894. 62. O'Neill, E., Collins, D., López-Garriga, J., Macchiavelli, R., Cruz, J., Center for Resources in General Education (CIVIS): Towards Student Success in General and STEM Education. ASEE/IEEE Frontier in Education 2009, TIA-A7. 63. Ruiz, C., Nieves, C., Estremera, R., Lopez-Garriga, J., Jimenez, J. Ciencia sobre ruedas en Granada: Una experiencia de actualización científica y tecnológica en el aula. Enseñanza de las Ciencias, Investigación en Didáctica de las Ciencias, 2009, 1319-1322 64. Carlos A. Nieves-Marrero, Carlos R. Ruiz-Martínez, Rafael A. Estremera-And&uacutejar, Luis A. González, Juan López-Garriga and Jose A. Gavira. Two-Step Counterdiffusion protocol for the crystallization of Hemoglobin II from Lucina pectinata in the pH range 4 to 9. Acta Crystallograph Sect F Struct Biol Cryst Commun. 2010, F66, 264-268. 65. C. Ramos, R. Pietri, W. Lorenzo, E. Roman, L.Granell, C. Cadilla and J. López-Garriga. Recombinant hemoglobin II from Lucina pectinata: A large-scale method for hemeprotein expression in E. coli. The Protein Journal 2010, 29(2), 143 – 151. 66. Bonaventura, C., Henkens, R., W. Jesus, W., Lopez-Garriga, J., Jia, Y., Alayash, A., Parker , C., Crumbliss. A., Extreme Differences between Hemoglobins I and II of the Clam Lucina pectinalis in Their Reactions with Nitrite. BBA - Proteins and Proteomics (2010) 1804, 1988–1995 67. Román-Morales, E., Pietri, R., Ramos, B., Vinogradov, S,N., Lopez-Garriga, J. Structural determinants for the formation of sulfhemeprotein complexes. Biochemical and Biophysical Research Communications. (2010) In press. 68. Ruth Pietri, Elddie Román-Morales, Juan López-Garriga, Hydrogen Sulfide and hemeproteins: knowledge and mysteries. Antioxidants & Redox Signaling Forum Review (2010) In press.

Submitted Publications 1. Ruiz-Martinez, C., Nieves-Marrero, C., Estremera, R., Garcia-Caballero, A., Gavira, J., Lopez-Garriga, J., Garcia-Ruiz, J.M. A highly efficient screening methodology for protein crystallization based on the counter-diffusion technique. J. Applied Crystallography, November 2010

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Publications in process 1. Linear relation between FeC and CN modes for hemeproteinsCN complexes. Cruz-Balberdi, J. López-Garriga. 2. López-Garriga, J. Mercado-Feliciano, S. Expository Program K-20+: Enhancing Chemistry and Technology Education”

Graduate Students Thesis (Total 50, 46 MS and 4 PhD) 1. Factors Influencing the C=N Stretching Frequency: (C=N/C=N-H) Stretch-Bend Model or

Rehybridization Model. Juan E. Lastra Hernandez (M.S.) May 1993 2. Interaction Between Inner Membrane Mitochondria Cytochrome c Oxidase and Nitrous Oxide:

Spectroscopic Studies. Jesus Hernandez Baez (M.S.) May 1993 3. Time-Resolved Infrared Spectroscopy: Heme-proteins Photoligand Dynamics. Raul A.

López-Mejias (M.S.) May 1994 4. Determination of Volatile Halogenated Organic Compound in Tropical Marine Algae

Catalino C. Suárez Ramírez (MS.) May 1994 5. Determination of Volatile Halogenated Organic Compound in Tropical Terrestrial Ecosystems. Anibal

Quintana Cheeseborough (M.S.) May 1995 6. Spectroscopic Studies of Hemoglobin I from Lucina pectinata. Angela M. Navarro

Vargas (M.S.) May 1995 7. Ab-initio and Spectroscopic Studies on Protonated and Unprotonated Schiffs Bases

Model Compounds. Alejandro O. Cuello Ellena (M.S.) May 1995 8. UV/VIS and FTIR Spectroscopic Studies of Lyophilized Cytochrome c Oxidase in

Mitochondria Inner Membrane and Complexes. Alma G. Irizarry Zambrana (M.S.) May 1996 9. Taxonomic Studies on Extremely Halophilic Archaebacteria Isolated from the Solar

Salters of Cabo Rojo, Puerto Rico. Rafael Montalvo Rodriguez. (M.S.) May 1996 10. 1H NMR Studies of the Lucina pectinata Met-Cyano and Met-Hydrogen Sulfide

Hemoglobin I Complexes. Eilyn Raquel Silfa Olaverria (M.S.) May 1997 11. Resonance Raman Spectroscopy of Deoxy, Oxy, and Carbon Monoxy Hemoglobin I

from the Clam Lucina pectinata. Jose F. Cerda Colon (M.S.) May 1997 12. Resonance Raman Spectroscopy of Cyano and sulfide Hemoglobin I Complexes

from the Clam Lucina pectinata. Yolanda Echevarria (M.S.) May 1997 13. 1H NMR Spectrum Assignment of the Heme Pocket of HbI-CO Complex from Lucina pectinata.

Maritza Almeida (M.S.) May 1997 14. Crystallization 1-Br, 2-Naphthylidene-n-methylimina and its Protonated Derivatives.

Patricia M. Osorio Suarez (M.S.) December 1997 15. Determination of Volatile Halogenated Organic Compound and Dimethylsulfide in Open Sea in Tropical Environmental Systems Domingo Acosta (M.S.) December 1997 16. Determination of VHOC in Tropical Marine Systems by SPTD-SM and GC-MS. Breda Cruz, (M.S.) December 1997

17. Biodegradation of Organophosphorus Compounds by Extremely Halophilic Bacteria. Celia R. Quiñones-Seiglie (M.S.) May 1988

18. Isolation and Characterization of Carotenoids from New Halophilic Bacterial Species. Anuja Katiyar (M.S.) May 1999

19. Biodegration of Volatile Organic Compounds by New Extremely Halophilic Bacteria: Solid Phase Microextraction and GC/MS. Maria Isabel Meléndez Santana (MS) May 1999

20. Cloning and Sequencing of cDNAs Encoding Hemoglobin I from Lucina pectinata. Frances Antomnattei (M.S.) July 1999 21. Isolation and Purification of Lucina pectinata Hemoglobins using Preparative Isoelectric Focusing. Zuleica Sanoguet (M.S.) December 1999

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22. Femtosecond Spectroscopy Studies of Carbon Monoxide Hemoglobin I Complex from Lucina pectinata. Carlos Ramon Ruiz-Martinez (M.S.) May 2000

23. Femtosecond Spectroscopic Studies of Cyanide Hemoglobin I Complex from Lucina pectinata. Ruth Pietri Meléndez (M.S.) May 2000

24. Reactions of Hemoglobin I from Lucina pectinata with Hydrogen Peroxide: The Formation of the Ferryl Intermediate. Walleska W. De Jesus Bonilla (M.S.) May 2000

25. Resonance Raman of Heme-Ligand Isotopic Complexes of HbI from Lucina Pectinata. Erick Morales (M.S.) December 2000

26. Cloning of HbI cDNA from Lucina pectinata into a Prokaryote His-Tagged Expression Vector. Tanya Rosado, (M.S.) December 2000

27. Visible Light Sensitization of Titanium Dioxide by Copper Phathalocyanine. Erick Acosta Otero (M.S.) December 2000

28. Encapsulation and Ligand Reaction of Hemoglobin I from Lucina Pectinata in Sol-Gels. Lymaries Estarda (M.S.) May 2001

29. FTIR Studies of Ferrous and Ferric Hemoglobin I Cyanide Complexes from Lucina pectinata. Yohmary Ortiz Pijuán (MS) July 2001

30. Expression, Purification and Site-directed mutagenesis of HbI from Lucina pectinata Vanessa N. Collazo Vélez (MS) July 2001

31. Investigation of the Hemoglobin I Carbonmonoxide and Oxy Complexes from Lucina pectinata by Femtosecond transient Absorption. Cacimar Ramos (MS) May 2002 32. NMR Investigation of the influence of Distal Gln E7 on the Structural Properties of Lucina pectinata. Brenda Judith Ramos Santana (MS) May 2003 33. The cDNA-Derived Amino Acid Sequence of Hemoglobin II from Lucina pectinata. Elineth Torres Mercado (M.S.) May 2003 34. Functional Characterization of the Purified Hola Form of HbI from Lucina pectinata Over expressed in Escherichia Coli. Evys A. Collazo Santigo (MS) May 2003 35. Theoretical calculations of the cyanide ligand behavior in HbI from Lucina pectinata. Lisa Torres González (MS) June 2003 36. Distal Heme Pocket Interactions and Analysis of cysteine sites in Hemoglobins (I, II, and II) cyanide complex from Lucina pectinata. Johana Ramos Lorenzo (MS) June 2003 37. Ultrafast time-resolved absorption study of ferrous, ferric, and ferryl hemoglobin I ligand complexes of Lucina pectinata. Eunice Ramírez-Meléndez (MS) December 2003 38. Ligand Stabilization in the cyanide hemoglobin (I, II, III) complexes from Lucina pectinata. Anthony Cruz-Balberdi. (MS) December 2003 39. Ligand-binding control in HbI from L.pectinata: Structural implications of B10, E7 and E11 mutants. Ruth Gretchen (PhD) in collaboration with Dra. C. Cadilla (Director), December 2004 40. The Role of Ultrafast Events of Hemoglobin I and II Ligand complexes in Lucina pectinata. Maria M. Rodriguez Guilbe (MS) June 2005. 41. Effect of the E11 on the ligand binding in HbI from L. pectinata Laura Granell-Ortiz (MS) June 2007 42. The role of position E11 in the stabilization of the HbI-SH2 complex from L. pectinata Yahaira López-Cuevas (MS) December 2007 43. Assessment of the stability and biological activity of model proteins encapsulated in hydrogels. Celimar Valentin (MS) in collaboration with Dr. Madeline Torres Director, Dec. 2007 44. Expository programs: A model to influence learning in Chemistry, Samirah Mercado (MS) Dec. 2007. 45. Oxidation Reactions of Lucina pectinata hemoglobins: Model system to desing heme proteins based blood substitutes. Wallesla De Jesus-Bonilla (PhD), June 2008 46. Mechanism of sulfhemoglobin formation. Elddie Marie Roman (MS), June 2008 47. Detection of hydroperoxy complex in the oxidative reactions of myoglobin with hydrogen peroxide. Hector Arbelo (MS), December 2008 48. Expression of Recombinant Hemoglobin II from L. pectinata. Wilmarie Lorenzo (MS), June 2009 49. Characterization of the Genes Coding for Hemoglobins from Lucina pectinata Linda Edith Rivera (PhD) in collaboration with Dra. C. Cadilla (Director) June 2009 50. Hemoglobin I from Lucina pectinata: A model for hydrogen sulfide reactivity within hemeproteins, Ruth

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Pietri Meléndez (PhD) June 2009.

National and International Invited Seminars Presentations (Total 32) 1. "Rehybridization of the C=N Bond Upon Protonation of Schiff's Bases Increases the Stretching Frequency". Los Alamos National Laboratory, INC-4, Los Alamos, N.M April 1988 2. "Time-Resolved Infrared Observation of a CuB+-CO Phototransient in Bovine Cytochrome Oxidase at 300K" R.B. Dyer, O. Einarsdóttir, P.M. Killough and W.H. Woodruff. Gordon Research Conference "Metals in Biology" Ventura, California January 1989 3. "Transient Absorbance, Time-Resolved Infrared and Time-Resolved Resonance Raman Studies: Photodissociation of ligands from Cytochrome Oxidase", Harvard Medical School, Wellman Laboratory,

Massachusetts. July 1989 4. "Novel Applications for FT-Raman" Second Pan American Chemical Congress, San Juan, P.R. Sept. 1991 5. Orientation of the Fe-CO Moiety in Heme-CO Complexes" XX-Latin American Chemical Congress, Santo Domingo May 1992. 6. "Interaction of Heme-Proteins with Compounds from Tropical Marine Environments: A Bioactive Probe" Marine Natural Products. La Pargera P.R. March 1993 7. "Spectroscopic Techniques Applied to Biotechnology" 13th International Chemical Education Conf." San Juan P.R. August 1994 8. Applications Time-resolved IR Spectroscopy.NSF-International Workshop, Foz do Iguacu, Brazil.May 1996 9. Structural Events in Hemoglobin I Ligand Complexes of Lucina pectinata XIIth International Conf. on Invertebrates Dioxygen Binding Proteins, Roscoff, France, July 2000 10. GLOBE program in Puerto Rico: Implementation Panel. Six Annual GLOBE Conference, Blaine, Washington State. July 2001 11. “GLOBE and Outreach programs in Puerto Rico” Texas Science Summit, San Antonio, Texas. EU January 2002 12. R. Camacho-Zapata, J. López-Garriga. Science on Wheels Educational Center: An integration of Outreach Programs from Industry, Government, Academia, and Scientific Societies. 7th Annual GLOBE Conference, Washington July 2002 13. “Science on Wheels Educational Center: An integration of Outreach Programs from Industry, Government, Academia, and Scientific Societies”. Science Education Department, Virginia Commonwealth University. VA. October 2002 14. “Hemoglobin I from Lucina pectinata: Structure, Function, and Technological Applications”. Chemistry Department, Virginia Commonwealth University. VA. October 2002 15. Students in Outreach programs. Duke Marine/Freshwater Biomedical Center, Beaufort, NC April 2003 16. “Structure and Technological Applications of HbI from L. pectinata” Department of Physiology and Biophysics Albert Einstein College of Medicine Bronx, NY April 2003 17. Structural Events in HbI from Lucina pectinata. Barcelona Scientific ParK,Barcelona, Spain July 2003 18. Graduates and Undergraduate Students Leadership in Pre-College Education, University of Virgen Island in Saint Croix. March 2004 19. Hemoglobins Ligand Complexes of Pectinata, University of Virgen Island in Saint Thomas. March 2004 20. Structural Events of Hemoglobins Ligand Complexes from Lucina Pectinata, Chemistry Division, Argonne National Laboratory, Chicago, IL. June 2004. 21. Expository Programs : Enhancing Science Education to the Public. Parque de las Ciencias, Granada, Spain. May 2006 22. Expository Programs: Enhancing Chemistry and Technology Education. Rutgers University. April 2006 23. Expository Program K20+: Enhancing Chemistry and Technology Education. Holly Cross University, Pennsylvania. April 2006 24. Expository Programs: An Approach to Enhance Science Education.Caribbean Institute for Bio-Exploration (CIBEX), Santo Domingo, Dominican Republic, June 2006 25. Expository Programs: An Approach to Enhance Science Education. Expanding opportunities for K-12 students Meeting. Rutgers University. October 2006

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26. Expository Programs(II): Enhancing Science Education to the Public. Parque de las Ciencias, Granada, Spain. August 2007 27. Hemoglobins of L. pectinata: Structure and function relation. CSIC, Granada, Spain. August 2007 28. The GLOBE program in Puerto Rico. Centro de Investigación y Documentación Educativa (CIDE), Ministerio de Educación y Ciencia, Madrid. December 2007. 29. The NSF GK-12 of the University of Puerto Rico at Mayaguez. Universidad Politecnica de Catalunya. Barcelona, Spain. December 2007. 30. The NSF GK-12 of the University of Puerto Rico at Mayaguez, Centro de documentación i experimentación en ciencias i tecnología. Barcelona, Spain. December 2007. 31. Heme- proteins structure and function. Parque Ciencias de Granada, Spain. Sept 2008 32. International Activities in reseach and education, GK-12 conference NSF, Washington, D.C. March 2009

Puerto Rico Invited Seminars Presentations (Total 71) 1. Rehybridization Mechanism Nitrogen Electron Lone Pair" UPR, Rio Piedras P.R June 1988 2. Time-Resolved Infrared Observation of a CuB+-CO Phototransient in Cytochrome Oxidase" Chemistry Department, University of Puerto Rico, Mayagüez, P.R May 1989 3. Time-Resolved Infrared Observation of a CuB+-CO Phototransient in Bovine Cytochrome Oxidase at 300K" Chemistry Dept., UPR May 1989 4. Cytochrome a3 is Low Spin Following Photodissociation of CO from Reduced Cytochrome Oxidase". Chemistry Department, U.P.R Mayagüez. September 1989 5. Lasers and Chemistry" A.C.S. University of Puerto Rico, Mayagüez. September 1990 6. Halogenation Processes in Tropical Marine Biota" Marine Science Department,UPR-Mayagüez Aug 1991 7. Dynamics Photoligands in Cytochrome Oxidase" Symposium Apply Chemistry UPR Arecibo May 1992 8. "Time-Resolved Infrared Spectroscopy and Proteinquakes" Laser EPSCoR June 1992 9. "Ligand in Cytochrome Oxidase" Catholic University of Puerto Rico. October 1992 10. "Structural Relations in Cytochrome Oxidase" Ponce School of MedicineDec. 1992 11. "Science Shows and Chemistry" A.C.S., UPR, Mayagüez Campus September 1993 12. Undergraduate Research in Chemistry@ UPR-Mayagüez. October 1995 13. Science on Wheels. National Chemistry Week, URP Ponce. P.R. November 1995 14. Importance of Re-Training K-12 Teachers@ Carmen Salas, Jayuya P.R. March 1996 15. Lasers in Science Colegio San Jose (7-12 grade) San German. P.R. October 1996 16. GLOBE: Global Learning and Observations to Benefit the Environment. 8th Conferencia Estatal para el Mejoramiento Profesional de los Maestros de Ciencias y Matemáticas, Departamento de Educación, Gobierno de Puerto Rico, Río Grande, PR. November 1997 17. GLOBE: An International Educational Program Tercer Encuentro Regional de Educación y Pensamiento. University of Puerto Rico at Mayagüez. May 1998 18. GLOBE: An International Interdisciplinary Programa. 1er Congreso Caribeño en Tecnología en la Enseñanza de las Ciencias y Matemáticas. NASA-Mayagüez, P.R. May 1998 19. Structural Events and Technological Applications of HbI from Lucina pectinata Chemistry Department, UPR-Mayagüez August 2000 20. Hemoglobins of L. pectinata: Evolution to Technology. Biotechnology Day, Mayagüez, P.R. May 2001 21. Hemoglobins as Artificial oxygen Carrier Systems: Studies, Advantages, and Limitations. 60th Chemical Conference, Colegio de Quimicos Rio Grande, P.R. August 2001 22. Biophysical Studies of HbI from L. pectinata. Medical School University of Puerto Rico. August 2001 23. Global Learning and Observations to Benefit the Environment: Teachers Enhancement in Science and Technology (GLOBE-TEST). Puerto Rico 1st Space Grant Consortium, Cerromar , Dorado, P.R. Nov. 2001 24. Technological and biophysical Studies of HbI from Lucina pectinata. University of Puerto Rico, Aguadilla, Campus, P.R. March 2002 25. Science on Wheels Educational Center: An integration of Outreach Programs Industry, Government, and Academia Pfizer Inc. Education Week Dorado P.R May 2002 26. Science and Technology for Students Retention” 8th Annual del College Board. San Juan,P.R..Oct. 2002 27. Hemoglobin I from L. pectinata: Structure and Technological Applications. Chemistry Department,

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ACS-Student Chapter, Mayagüez, P.R. October 2002 28. R. Camacho-Zapata and J. López -Garriga. Science and Technology: Key elements for the retention of k-12 students, 8th Annual College Board Conference, San Juan October 2002 29. C. Lugo, M. López, R. Camacho-Zapata, and J. López -Garriga. Science Shows and Demonstrations. Puerto Rico Space Grant Convention, San Juan Puerto Rico October 2002 30. New Opportunities for Collaborative Biomedical Research in Academia” Latin American and Caribbean Biotechnology Congress, Mayagüez Resort and Casino, PR. November 2002 31. Why to study is important. College Carrier Day, Community School Blanca Malaret, Sabana Grande, Puerto Rico February 2003 32. Curricular Innovation in the Chemistry Curriculum. INDUNIV-CTP. P.R. April 2003 33. Structural Events and Technological Applications of Hemoglobins Ligand Complexes from L.Pectinata. Bioengineering Colloquium. Mayagüez Resort and Casino, PR. May 2003 34. Science and technology Enterprises, Club Rotarios, San German, PR. October 2003

Graduate and Undergraduate Students Leadership in Pre-College Environments, ACS 27TH Senior Technical Meeting, Quebradillas, P.R. November 2003 35. Challenges for graduate and undergraduate chemistry students. ACS Chapter-University of Puerto Rico at Arecibo, Arecibo. P.R. December 2003 36. Graduate and Undergraduate Students Leadership in Pre-College Environments, Metropolitan University, Project FEST, San Juan. April 2004 37. Structural Events of hemoglobin ligand complexes from L. pectinata. J. López -Garriga, C. Cadilla. Spring Conference in Protein Research, COBRE II-NIH Center, San Juan. April 2004 38. Research and environmental education. UMET, San Juan P.R. April 2004 39. Chemical events in Hemoglobins from L. Pectinata. Physics Department, University of Puerto Rico, Mayagüez P.R. April 2004 40. Calculator bases laboratory a Teachers Enhancement in Science and Technology. PROMISE-TEST, Biology Department, University of Puerto Rico, Mayagüez P.R. May 2004 41. Structural Events and Technological Applications of Hemoglobins from the symbiosis of Lucina Pectinata. Department of Agriculture UPR-Mayagüez September 2004 42. Hemoglobins’ Symbiosis of Lucina pectinata. Biology Honor Society. Biology Department, University of Puerto Rico, Mayagüez P.R. November 2004 43. Raman and resonance Raman spectroscopy in biological systems. Puerto Rico Medical School, San Juan , P.R. March 2005 44. K-20+ efforts in Biotechnology: a model for pre-college education. Invitation on a press conference on Biotechnology by the Governor of Puerto Rico, Anibal Acevedo Vila. March 2005 45. Symbiosis Processes in Lucina pectinata. GLOBE workshop, Chemistry Deparment, University of Puerto Rico, Mayaguez Campus, Mayaguez P.R. June 2005 46. The chemistry at the house. 3er ACS Students initiation, UPR-Aguadilla, Campus, P.R. October 2005. 47. Chemistry History. Puerto Rico Academy of Arts and Sicences. University of Puerto Rico, Mayaguez Campus, Mayaguez P.R. October 2005. 48. Expository Program K20+: Enhancing Chemistry and Technology Education. UPR-Utuado Campus, Utuado, Puerto Rico. Mayo, 2006 49. Outreach Programs of the Science on Wheels Educational Center. Fideicomiso de Puerto Rico, San Juan, P.R., June 2006 50. Expository Programs: An Approach to Enhance Science Education, Pfizer- Feria de la Salud, San Juan P.R. September 2006 51. La Quimica en las Americas, ExpoChem 2006, UPR-Mayaguez, P.R. November 2006 52. Heme oxidation state control in the hemoglobins from Lucina pectinata: Structural implications for ligand selection. Chemistry Department, UPR-Humacao. March 2007 53. Heme oxidation state control in the hemoglobins from Lucina pectinata: Structural implications for ligand selection. Biology Department, UPR-Arecibo April 2007

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54. Science shows an approach to enhance Chemical Educaction. Industrial and Educational Chemical Association of the Commonwealth of Puerto Rico. August 2007 55. Science Demonstrations and Chemical Educaction. 4to Congreso Alianzas Educativas Pfizer. Hotel Caribe Hilton, San Juan. Puerto Rico. September 2007 56. Science shows and Science education. SALUDexpo Coliseo Roberto Clemente, San Juan. Sept 2007. 57. Science demostrations and the impact in education. Parque Criollo de Ciencias y tecnologia. Caguas, Puerto Rico. November 2007 58. Chemistry Demonstrations for Special students. Chemistry Department, UPR-Mayagüez. Jan. 2008. 59. The GLOBE program at UPR-Mayagüez. NASA-TCESS, Electrical Engineering Department, University of Puerto Rico at Mayagüez. February 2008 60. Heme oxidation control in hemoglobins from Lucina pectinata: Structural implications for reactivity. Ana G. Mendez University System, Cupey, P.R.. February 2008 61. Heme oxidation control in the hemoglobins from Lucina pectinata: Structural implications for reactivity. Turabo University. P.R. February 2008 62. Understanding science evolution. Pre-college research symposium, UMETUniversity, Ana G. Mendez University System, Cupey, P.R. May 2008 63. Science and the Public. GLOBE worshop for teachers, sponsor by Pfizer, UPR-Arecibo, June 2008 64. Oxidation hemeproteins-glucose oxidase. Life-Scan Johnson and Johnson, Aguadilla, P.R. July 2008 65. Science for the Public. ACS Local Section “Science Café” La Terlulia, Mayaguez, P.R. Sept. 2008 66. Successful research and education proposal writing. Research and Developmet Center, UPR-Mayaguez, P.R. October 2008 67. Science for the Public. ACS-Puerto Rico Local Section “Science Café”,Camuy, P.R. October 2008 68. Invited Speaker ACS-Student Chapter University Puerto Rico, Mayagüez. October 2008 69. Pre-university research for teachers and estudents, School Distric, Mayaguez, Puerto Rico. Nov. 2008 70. The role of H2S in Invertebrate organism. Summer NASA Camp. Cabo Rojo.Puerto Rico. July 2009 71. Undergraduate research initiatives at universities, centers and national laboratories, Society of Hispanic Professional Engineers (SHPE), UPR- Mayagüez, PR, November 2010

National and International Presentations in Technical Meetings (Total 60) 1. "Laser Raman Spectroscopic Study of Lectin-Sugar Interactions" López -Garriga, J. and Jao, T.C. 8th NIH-MBRS , Albuquerque, New Mexico. April 1981 2. "Aromatic Schiff's Bases and Frequency Shift on the C=N Stretching Frequency Upon Protonation and Deuteration". J.J. López-Garriga, G.T. Babcock and J.F. Harrison. Joint Great Lakes and Regional Meeting of A.C.S. Kalamazoo,USA, May 1984 3. "Factors Influencing the C=N Stretching Force Constant in Schiff's Bases" J.J. López-Garriga, G.T. Babcock 29th Ann. Meeting Biophysical Society, Baltimore. March 1985 4. "Rehybridization of the C=N Bond Upon Protonation of Hydrogen Cyanide Increases Stretching Frequency" J.J. López-Garriga, G.T.Babcock ,XI Ann. Midwest Chem.ANL May 1988 5. "Time-Resolved Infrared Studies of Phototransient CO-Cytochrome Oxidase" R.B. Dyer, J. López -Garriga, O. Einarsdóttir, and W.H. Woodruff. 4th International Conference On Time-Resolved Vibrational Spectroscopy, Princeton, New Jersey, USA. June 1989 6. "Time-Resolved Infrared Studies of the Binding and Photodynamics of CO in Carbonmonoxy Complexes of Cytochrome Oxidase" W.H. Woodruff, R, J. López -Garriga, O. Einarsdóttir, and P.Killough, 4th Inter.Conf. TR Vibrational Spect.,Princeton, NJ June 1989 7. "Time-Resolved Infrared Linear Dichroism Studies of the Orientation of CO in Complexes of Cytochrome Oxidase" W.H. Woodruff, R.B. Dyer, J. López -Garriga, O. Einarsdóttir, 4th International Bioinorganic Chem, MIT, Cambridge, Massachusetts July 1989 8. "Photodissociation and Recombination Dynamics of Carbonmonoxy Cytochrome Oxidase" O. Einarsdóttir, R.B. Dyer, J. López -Garriga, O. Einarsdóttir, P.M. Killough, and W. Woodruff, 4th International Conf. On Bioinorganic Chemistry, M.I.T.,Cambridge,USA July 1989 9. The FeCO Vibrational Frequencies and the FeCO Angle in Heme-CO Complexes J. López -Garriga, W. Woodruff, W.A. Oertling, G.T. Babcock, 2nd Pan American Chemical Congress San Juan, September 1991

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10. "N2O Interaction With Bovine Heart Inner Mitochondria Membrane " J. López -Garriga, M. Rivera J. Hernandez, ASBMB/DBC-ACS Meeting, San Diego, CA. May 1993 11. "Electron Transfer Reactions: Lucina pectinata's Hemoglobin I" J. L. Colon, M. Maldonado, A. Navarro,J. López -Garriga 207th ACS National Meeting, San Diego March 1994 12. "Characterization of the CO complex of HbI and its electron-transfer properties". A. Navarro J. López -Garriga, J.L. Colon. 210th ACS Nat. Meet. Chicago August 1994 13. "Structure-Function Relationship in Hemoglobin (HbI) from Lucina pectinata" Seven Time-Resolved Vibrational Spectroscopy Conf." Santa Fe, New Mexico. R. López-Mejia , J. López –Garriga. June 1995 14. "Science on Wheels: A Link Between Educational Cultures" J. López -Garriga, I. Munoz, Y. Echevarria. Chem.Ed. 1995, Old Dominion Univ. Norfolk, Virginia . August 1995 15. "Characterization of Ligand Binding and Electron Transfer Properties of Lucina pectinata HbI. M. Maldonado, A. Navarro, R. Leon, J. López -Garriga, J.L. Colon. Seventh International Conference on Bioinorganic Chemistry, Lübeck, Germany. September 1995 16. Spectroscopic Characterization and Molecular Modeling Studies of L. pectinata HbI. M. Maldonado, A. Navarro, J. López -Garriga, J.L. Colon. 3rd Panamerican Chemical Congress, San Juan, P.R. September 1995 17. Sea-Atmosphere Interaction in the Tropics. CO2 in the Oceans International Symposium. University of

Puerto Rico, Mayagüez Campus, Mayagüez P.R. January 1996 18. CO Dynamics in Hemoglobin I from Lucina pectinata. 8th Interamerican Photochemical Society Conference, Foz do Iguacu, Brazil . May 1996 19. Science on Wheels: An Integrated Pre-college Outreach-Program. 5th Chemical Congress of North America, Cancún, Mexico. November 1997 20. What can a clam tell us about binding in heme proteins J.L. Colon, M. Maldonado, R. Leon, A, Marti, A. Calcaño, J, López -Garriga, Metal in Biology Gordon Conference, Ventura, California, January 1998 21. Biological Applications of Time-resolved Infrared Spectroscopy. 17th IUPAC Symposium on Photochemistry. Sitges (Barcelona, Spain) p374, July 1998 22. GLOBE and Science on Wheels Programs: International Science Outreach Initiatives. J. López Garriga, W. De Jesus, R. Camacho, W. Nazario, D. Mora, M. Camacho, and J. Fuentes 15th Biennial Conference Chemical Education, Waterloo, Canada. August 1998 23. GLOBE: An International-Interdisciplinary Educational Program. J. López -Garriga, R. Camacho, W. Nazario, W. De Jesus NASA University Research Centers, Earth Systems Sciences and Education. (T. Coleman, et al.,. ISBN:1-889335-07-x) Vol. III pag. 784-788, 1998 24. Probe studies of the Unique Heme Pocket Microenvironment of the Sulfide Transport HbI of Lucina pectinata. J.L. Colon, M. Maldonado, R. Alarcon, R. Leon, A, A. Calcaño, J. López -Garriga XXXIII Inter. Conference on Coordination Chemistry, Florence, Italy Aug 1998 25. Biodegration of Organophosphorus Compounds by Extremely Halophilic Bacteria. Celia R. Quiñones-Seiglie and Juan López -Garriga 218th ACS National Meeting, Boston, 1998 26. Ultrafast Absorption Measurements of Hemoglobin I- Carbon Monoxide complex from L. pectinata. J. López -Garriga, C. Ruiz, R. Pietri, K. Rodriguez. Femtochemistry IV Leuven, Belgium. July 1999 27. GLOBE: Interdisciplinary Education to Enhance K-12 Curriculum J. López -Garriga, R. Camacho NASA University Research Centers: Technical Advances in Aeronautics, Space Sciences and Technology, Earth Systems and Education 2000 28. Structural, Dynamical, and Technological Events of Hemoglobin I from L. pectinata R. Pietri, C. Ramos, W. De Jesús, E. Ramírez, M. Chergui, J. López-Garriga. Symposium in Honor to Prof. Gerald T. Babcock. MSU, East Lansing MI. June 2001 29. The Graduate and Undergraduate Students Teaching Acquaintance and Leadership Behind Chemistry Outreach Programs. R. Camacho-Zapata, J. López-Garriga Prof. Gerald T. Babcock Seminar series MSU, East Lansing MI. June 2001 30. Status of the GLOBE program in Puerto Rico. R. Camacho-Zapata, M. Colombani, J. López-Garriga Six Annual GLOBE Conference, Blaine, Washington State. July 2001 31. Nonhydrogen Bonded Compound II in the Cyclic Reaction of Hemoglobin I from Lucina pectinata with Hydrogen Peroxide” W. De Jesús-Bonilla, E. Ramírez J. Cerda, J. López-Garriga. International Conf. On Bioinorganic Chemistry, Florence, Italy. August 2001 32. Heme Reduction and Ultrafast Geminate Recombination Cyanide HbI L. pectinata R. Pietri, C. Ruiz, C.

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Ramos, J. Helbing, M. Chergui, J. Lòpez-Garriga, Vth Femtochemistry Confer, Toledo, Spain.bSept. 2001 33. Status of the GLOBE program in Puerto Rico, R. Camacho-Zapata, J. López-Garriga Seventh Annual GLOBE Conference. Lake Front Chicago, Illinois. July 2002 34. Ultrafast UV-Visible and Infrared Study of the Cyano Complexes of Mb and HbI from L. Pectinata J. Helbing, J. Bredenbeck, P. Hamm, R. Pietri, C. Ramos, J. López-Garriga, F. Chaussard, A. González, F. van Mourik and M. Chergui, Femtochemistry VI, Paris, July 2003 35. Oxygen and carbon monoxide binding kinetics of HbI from Lucina pectinata R.G. Leon, L.Kiger, C.L. Cadilla, J. Juan López-Garriga. XIIIth International Conference on Invertebrates Dioxigen Binding Proteins, Mainz, Germany. September 2003 36. Structural events of HbI Ligand complexes from L. pectinata, López –Garriga, J SERM-ACS 2003, Atlanta. November 2003 37. Graduate and Undergraduate students enhancing Science and technology in GK-12 schools. Annual Meeting for GK-12 Projects Teams, Washington DC. March 2004. 38. Theoretical Study on the interactions of O2, and SH2 with Hemoglobins, Daniel E. Bacelo, Robert C. Binning, Jr, Carmen Cadilla, Juan López -Garriga. 2004 ISQBP President's Meeting, International Society of Quantum Biology and Pharmacology, Como, Italy, June 2004 39. Ultrafast Photophysical Processes of Fe(II) and Fe (III) HbI Ligand Complexes from L. pectinata. XX IUPAC Symposium on Photochemistry, Granada, Spain, July 2004 40. Processes of Fe(II) and Fe (III) HbICN Complexes from L. pectinata. 56th Southeastern Meeting of ACS (SERMACS), Research Triangle Park, NC.Nov. 2004 41. Conformational Processes of HbI-Mutants Ligand Complexes from L. pectinata, 2nd Colloquium Protein Structure, Function, Dynamics Caribe Hilton, San Juan, P.R February 2005 43. Graduate and Undergraduate students enhancing Science and technology in GK-12 schools. Annual Meeting for GK-12 Projects Teams, Washington DC. March 2005 44. Heme oxidation state in the hemoglobins from Lucina pectinata: Structural implications for ligand selection. 3nd Colloquium Protein Structure, Function, Dynamics San Juan, P.R March 2006 45. Expository Programs: An Approach to Enhance Science Education.. 231st ACS, Atlanta GA March 2006 46. Graduate and Undergraduate students enhancing Science and technology in GK-12 schools. Annual Meeting for GK-12 Projects Teams, Washington DC. March 2006 47. The role of structure in ultrafast dynamics of hemoglobins-ligand complex from L. pectinata. 33RD Meeting American Society for Photobiology. Rio Grande, P.R. July 2006 48. Expository Programs: An Approach to Enhance Science Education, 19th Biennial Conference on Chemical Education, Purdue Univ. Indiana. July-August 2006. 49. Crystallization and Molecular Structure of Hemoglobin II from L.pectinata, XIV International Conference on Dioxygen Binding and Sensing Proteins. Stazione Zoologica Anton Dohrn, Naples, Italy, September 2006 50. Graduate and Undergraduate students enhancing Science and technology in GK-12 schools. Annual Meeting for GK-12 Projects Teams, Washington DC. March 2007 51. Torres-Lugo, M., Lopez-Garriga, J. The Effect of Pre-Polymeric Solution and Subsequent Encapsulation in Hydrogel Membranes on the Bio-logical Activity of Myoglobins, 29th International Conference of the IEEE Engineering in Medicine and Biology Society and Biennial French Society of Biological and Medical Engineering, Lyon, France, August, 2007. 52. K-20+ Expository Programs: Enhancing Chemsitry Education, 234Th ACS National Chemical Meeting, Boston, MA. August 2007. 53. GK-12 Expository Programs: Enhancing Science and Technology Education. ICEE-Coimbra, Portugal. September 2007 54. Graduate and Undergraduate students enhancing Science and technology in GK-12 schools. Annual Meeting for GK-12 Projects Teams, Washington DC. March 2008 55. Globe program and the learning of chemistry and environment. 235th ACS National Meeting, New Orleans, LA, April 2008. 56. H2S and heme-oxygen complex activation. 42 IUPAC Congress, Glasgow, England. August 2009 57. K-12 programs enhance skills of chemistry Instructors. 42 IUPAC, Glasgow, England. August 2009 58. López-Garriga, J., Martínez, C.Estremera, R., Nieves, C., Sampedro, C., Juan de Dios Jiménez Ciencias sobre Ruedas en Granada. Una experiencia de actualización científica y tecnológica en el aula. VIII

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Congreso internacional investigacion en la didactica de las Ciencias. Barcelona, Spain. September 2009. 59. Oxyheme activation by hydrogen sulfide. SERMACS, Convention Center, San Juan P.R. October 2009 60. Gold-nanoparticles and Lab-Quest. NISE Network-Wide Meeting, San Francisco, CA. October 2010

Presentations in Technical Meetings in Puerto Rico (Total 42) 1. "Raman Spectra of Biologically Important Seleno-Amino Acids". J. López -Garriga, T.C. Jao and W.E. Rudzinkski, 3rd ACSPR Senior Technical Meeting, Puerto Rico. December 1978 2. "Raman Spectra of Seleno-Amino Acids". J. López -Garriga, T.C. Jao and W.E. Rudzinkski, Annual Meet. ACS, UPR, PR. December 1979 3. "The Raman Spectra of Selenomethionine and Selenocystine" J. López -Garriga, T.C. Jao and W.E. Rudzinkski, 4th ACS Senior Tech. Meet., San Juan, Puerto Rico. December 1979 4. "A Computer Controlled Laser Raman Spectrometer for Study of biological Materials" J. López -Garriga, T.C. Jao 5th ACS-PR Senior Tech Meet., San Juan, December 1980 5. "Cytochrome a3 is Low Spin Following Photodissociation of CO from Reduced Cytochrome Oxidase" J.J. López-Garriga, O. Einarsdóttir, P.M. Killough, R.B. Dyer, S.J. G. Palmer, and W. Woodruff. EPSCoR-AAAS Meeting, Puerto Rico. September 1989 6. "Metal-Ligand Vibrations of Cyanoferric Myeloperoxidase: Evidence for a Constrained Heme-Pocket in Myeloperoxidase" J.J. López-Garriga, W.A. Oertling, R.T. Kean, H. Hoogland, R. Wever, and G.T. Babcock, Second Joint EPSCoR-AAAS Meeting, P.R. September 1990 7. "Rehybridization of the CN Bond in Schiff's Bases Upon Lewis Acid complex Formation" J. López -Garriga XIV Senior Technical Meeting A.C.S. PR. December 1990 8. "Spectroscopic and Ab-initio Studies of Bacteriorhodopsin Model Compounds" J. López -Garriga 4th. Joint EPSCoR-AAAS Mt., San Juan, P.R. October 1991 9. "Spectroscopic Studies of Bacteriorhodopsin Model Compounds" J. López -Garriga 15th ACS Senior Tech. Meet. Ponce, Puerto Rico. November 1991 10. "Behavior of the Nitrogen Electron Lone Pair Upon Protonation of Schiff's Bases: Electronic Structure" J. Lopez-Garriga 16th ACS Senior Tech. Meet. Mayagüez, P.R. Nov. 1992 11. "Time-Resolved Infrared: Applications to Transients Species" J. López -Garriga 5th. Joint EPSCoR-AAAS Meeting Mayagüez, P.R. January 1993 12. "Evidence for the Rehybridization Mechanism Upon Protonation Schiff's Bases" J. López-Garriga 17th ACS Senior Meet. Palmas del Mar . November 1993 13. "Dynamics of Ligand Binding to Hemeproteins Using Time-Resolved Infrared Spectroscopy" J. López -Garriga 5th. Joint EPSCoR-AAAS Mt. Puerto Rico May 1994 14. "Interaction of Heme-proteins with Ligands: Vibrational Studies" J. López -Garriga 18th ACS Senior Meet. Aguadilla, P.R. November 1994 15. "Spectroscopic Characterization of Lucina Pectinata's Hemoglobin I . J. Colon and J. López -Garriga. 18th ACS Senior Meet. Aguadilla, P.R. November 1994 16. "Determination of Volatile Organic Compounds from Tropical Environments" J. López -Garriga, 1st NASA-EPSCoR Conference, Ponce, P.R. February 1995 17. "Structure of Hemoglobin (HbI)-Ligand Complex from L. pectinata" J. López-Garriga, J. Colon and A. Navarro. 7th. Joint EPSCoR-AAAS Mt. San Juan, P.R. April 1995 18. "Structure of HbI from L. pectinata" J. López -Garriga, 19th ACS Senior Meet. Lajas , P.R. Nov. 1995 19. A Synergetic Behavior Between Rehybridization and Resonance Protonated Schiffs Bases. J. López -Garriga. 20th ACS Senior Meet. San Juan P.R. November 1996 20. Orientation of the Heme Peripheral Groups in the Sulfide Reactive Hemoglobin I of Lucina pectinata. J. López -Garriga. 21 th ACS Senior Meet. Lajas, P.R. November 1997 21. Structural Characteristics of the Heme Chromophore of HbI from Lucina pectinata. J. López -Garriga, 22th ACS Senior Meeting, Aguadilla, P.R. November 1998 22. Advanced Photocatalytic Systems: sensitization of Metal Oxides. Erick Acosta and J. López-Garriga, 11th. Joint EPSCoR-AAAS Mt. Ponce, P.R. April 1999 23. Ultrafast Absorption Studies of HbI-Ligand Complex from Lucina pectinata.J. López -Garriga, 23th ACS Senior Meeting, Humacao, P.R. November 1999

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24. Structural Events and Technological Applications of Hemoglobin I from Lucina Pectinata. J. López -Garriga 31th Annual Senior Technical Meeting, Aguadilla, P.R. Nov. 2000 25. Hands-on Chemistry Demonstrations and the Global Learning and Observations to Benefit the Environment (GLOBE) Program" J. López -Garriga . 31thSenior Technical Meeting, Aguadilla, P.R. Nov. 2000 26. University of Puerto Rico Center for Research in Protein Structure, Function, and Dynamics” J. López –Garriga, M. Vera . 14th EPSCoR Meeting, Dorado P.R. May 2002 27.Development for the New Generation of Oxygen Carrier Substitutes: The cyclic Reaction of HbI with H2O2. ACS 26

th Senior Tech. Meet. San Juan, P.R. August 2002 28. Structural Events of Hemoglobins Ligand Complexes of L. pectinata. J. Lopez-Garriga. Spring Conference in Protein Research, Center of Proteins Structure, Function, and dynamics, San Juan, April 2004 29. Oxygen and Carbon monoxide binding kinetics of recombinant and site-direct mutants of L. pectinata HbI. Carmen Cadilla, R.G. Leon, L. Kiger, J. López -Garriga. Spring Conference in Protein Research, COBRE II-NIH –UPR Center, San Juan. April 2004 30. Molecular Studies of Proteins Encapsulated in Soft Materials. M. Torres, G. Lopez, J. Rios, J. López -Garriga, BRIN/INBRE symposium. San Juan, PR. June 2004 31. Factors controlling reactivity of hemeproteins compound I species. J. López Garriga, Walleska de Jesus. XXIX Senior Technical Meeting, Lajas, Puerto Rico, November 2005. 32. Heme oxidation in the hemoglobins from L.pectinata: Ligand reactivity. Spring Conference in Protein Research, COBRE II-NIH –UPR Center, San Juan. March 2006 33. La Quimica en las Americas. ExpoChem. UPR-Mayaguez, November 2006 34. Peroxidaxion of Hemoglobins, UPR- Research interaction in Bioscience, Mayaguez, P.R. December 2006 35. Science & Shows. Biotechnology Summer Camp (UPR-Mayaguez). June 2007 36. Heme oxidations state control in the hemoglobins from L.pectinata: Ligand selection and reactivity. XXXI ACS Senior Technical Meeting, Mayaguez, Puerto Rico, November 2007 37. Expository programs: a way to enhance chemical education. XXXI Senior Technical Meeting, Mayaguez, Puerto Rico, November 2007 38. López-Garriga, J., Wymore, T., Arbelo, H., Brown, S.T., Formation of compound 0 species in the reaction of myoglobin with hydrogen peroxide: Implications for hemeprotein. 32nd Senior Technical Meeting of the ACS, Rincón, Puerto Rico. November 2008 39. Science on Wheels: To estimulate students’ retention, NIH-INBRE (PRAABRE), San Juan, March 2010 40. Science on Wheels Educational Center. CIVIS Project, Mayaguez P.R. September 2010. 41. Structural determinants in the formation fo sulfhemeproteins. Lopez-Garriga, J., Román-Morales, E., Pietri P., Ramos, B., Vinogradov, S. 34th Senior Technical Meeting, Mayagüez P.R. November 2010 42. Lab-quest application to high schools chemistry courses and science fairs. 34th Senior Technical Meeting, Mayagüez P.R. November 2010

Active Research Proposals: 1. NIH/MBRS program. Unraveling the Reaction between Heme (FeIV=O) Moiety and H2S: The Sulfhemoglobin Scenario $468,305 May 2007- April 2011 2. NSF/ Biophysics "Selective Reactivity of H2S with GlnE7 and HisE7 in Hemoglobins: Implications for Organisms. $1,000,000 January 2009 – June 2013

Active Collaborative Research Proposals 3. NSF/ DMR “Wisconsin - Puerto Rico Partnership for Research and Education in Materials”. P.I. Nelson Cardona, Co-PI, J. Lopez-Garriga, J. De Pablo , P.F Nealey September 2009 – August 2013. Total $3,500,000 Funding individual project ~$200,000

Active Collaborative Training Undergraduate –Graduate Educational Proposals: 1. RISE 2 BEST: RISE Enhancing Biomedical Sciences and Biomedical Engineering in Science and

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Technology” P.I. J. Lopez-Garriga, Co-Pi Carlos Rios, Miguel Castro, Eduardo Juan, Madeline Torres, Luisa Guillemar, August 2010- July 2014 $5,130,133

Active K-12 Collaborative Educational Proposals: 1. Title V Department of Education “Center for Resources in General Education: Towards Student Success in General and STEM Education at UPRM” P.I D. L. Collins, E. CoPI O'Neill-Carrillo, J. López Garriga, R. Macchiavelli. November 2008- October 2013. Total ~$2,000,000. Funding for individual project ~$300,000. 2. IdeA- Network for Biomedical Research Excellence (INBRE fase III) “Advancing Competitive Biomedical Research in Puerto Rico” Science on Wheels initiative individual project., $180,000 July 2009- June 2013.

Pending Proposals: None

Collaborative Research with Other Institutions Professor Carmen Cadilla, Ph.D., University of Puerto Rico, School of Medicine, Biochemistry Department. San Juan. P.R. “Site directed mutagenesis of Hemoglobin I from Lucina pectinata.” Professor Majed Chergui, Ph.D. Institut de Physique de la Matiere Condensee, Faculte des Sciences, BSP Universite de Lausanne, Ch-1015 Lausanne, Switzerland. Project “Fs visible and infrared study Hemoglobin I ligand complexes of Lucina pectinata”. Professor Juan Manuel Garcia Ruiz, Crystallographic Center, University of Granada Spain: Crystallization of Hemeproteins from L.pectinata.

Professor Denis Rousseau, Albert Einstein College of New York, N.Y. USA Project “ Heme Proteins”

Research proposal Granted: 1. Pittsburgh Supercomputer, Cray X-MP/48. "On the Molecular Basis for the C=N Frequency Shift Upon Protonation of Retinal Schiff's Bases." G.T. Babcock, J.F. Harrison,J.López -Garriga. January 1987-1988 2. NIH Travel Support "Fourth International Conference on Time-Resolved Vibrational Spectroscopy". Princeton, New Jersey, $ 900 May 1989 3. Research Initiation Program, University of Puerto Rico, Mayagüez Campus," Development of Time-Resolved Infrared Spectroscopy" $ 27,000 November 1989 4. EPSCoR I Program (UPR-NSF Program),"Time-Resolved Infrared Spectroscopy: Dynamics of Photoligands Mitochondrial Complexes" $ 37,954 Jan. 1990- 1991 5. MRIPG Program (NSF) "Molecular Structure and Function Relations of Transients Photoreceptors: Time-Resolved Infrared Studies" $ 11,819 June 1990 - June 1991 6. Puerto Rico Sea Grant Program, University of Puerto Rico, Marine Science "Halogenation Processes in Tropical Marine Systems" $ 3,800 September 1990 7. Research Initiation Program, University of Puerto Rico "On the Molecular Basis for the C=N Energy Shift Upon Protonation of Retinal Schiff's Bases. $ 2,000 October 1990 8. American Chemical Society, The petroleum Research Fund, "Time-Resolved Infrared Spectroscopy: Photoligand-Dynamics Relations of Heme-Proteins" $ 18,000 Oct. 1990 - 1992 9. COTTRELL Corporation "Time-Resolved Infrared Spectroscopy: Dynamics of Photoligands Complexes" $ 15,000 August 1991-1992 10. NIH/MBRS "Photoligand-Orientation Relations in Hemeproteins: The CO-Detoxification Problem" $ 208,234 (Dr. Manuel Hernandez Avila) March 1991-1994 11. EPSCoR II UPR-NSF "Halogenation in Marine Biota." $ 16,666 Feb. 1992-1993 12. Puerto Rico Laser Facility/EPSCoR II Program "Development of Time-Resolved Infrared Spectroscopy" $ 95,000. November 1992-1994 13. EPSCoR-DOE Planning Grant "Novel Catalytic Materials for the Conversion of CO2 into Useful Energy

Products" $ 15,000 (Dr. B. Weiner, Director) July 1992-1993 14. EPSCoR II UPR/NSF, Marine Biotechnology Component "Molecular Aspects of Interaction of Hemoglobin with Compounds from Marine Biota." $20,000 March 1993-1995

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15. Camille and Henry Dreyfus Scholar Award: $ 50,000 June 1994-1995 16. HBCU/MIs-ARMY Research Office "Structure-Function Relationships Between Ligands and Heme-Proteins: CO and NO Selection Threshold" $150,000 September 1993-1995 17. MRCE(NSF) "Structure-Function Relationships in Heme-Proteins: Interaction with Bioactive Species from Tropics $140,000 (Dr. Thomas Tosteson, Director) October 1993-1998 18. Research Improvement in Minority Institutions (RIMI-NSF) "Ultrafast Dynamical Processes Chemical Systems" $ 495,913 (Instrumentation) July 1995-1998 19. NSF-Biophysics (MCD9627773) Structural and Dynamic Characterization of Dual Ligand Binding (H2S

and O2) Processes in an Usual Hemoglobin. $ 195,946 June 1996-1999 20. DOE-EPSCOR "Catalytic Processes in Photooxidation, Combustion and Environmental Detoxification: Advanced Photocatalytic Systems: Sensitization of Metal Oxides. $ 145,000 October 1994-1999 21. NASA-EPSCoR-UPR MARINE-ATMOSPHERIC CHEMISTRY I: Tropical Sea-Atmosphere Measurements of VHOC $230,000 (Dr. J.Gonzalez, Dir) June 1994-2000 22. NSF-Molecular Biophysics: Modulation of the Heme Pocket Conformation or Dual Concerted Mechanism upon Substitution of Hydrogen Sulfide by Oxygen Binding to Unusual Hemoglobin? $ 240,000 June 1999-2002 23. NIH/MBRS program. An Unique H2S-Hemoglobin I Structure: A Site-Directed Mutagenesis Study $ 279,273 July 2000-June 2003 24. Centers of Biomedical Research Excellence - NIH (COBRE-NIH). Center for Research in Protein Structure, Function and Dynamics. (J. López -Garriga Interim PI in collaboration with M. Vera, C. Cadilla, B. Pastrana, E. Cora, K. Griebenow, G. Lopez, J.Rios, E. Melendez) $7,685,135 October 2001-2005 25. NIH/MBRS program. The Unique Hemoglobin I of L. Pectinata: A Study of the Heme (FeIV=O) Moiety $ 379,273 July 2003-April 2007 26. NSF/ Biophysics. Hemoglobin I from Lucina Pectinata: A Model forHydrogen Sulfide Reactivity within Hemeproteins. $450,759. January 2006 – June 2009 27. BRIN/INBRE- NIH. Molecular Studies of Proteins Encapsulated in Soft Materials. M. Torres (PI), G. López, J. Rios, J. Lopez-Garriga. $ 200,000. October 2004-2009

K-12 Educational (Pre-College) Proposals Granted 1. American Chemical Society, Project SEED, $ 5,600 Summer 1990 to 1993 2. Center for Science and Engineering Resources of Puerto Rico, "Undergraduate Research Summer" $ 2,600 May 1990 3. AMOCO Corporation. "Chemical Education" $15,000 March 1991 4. UPR, Program of the Center for Teaching Innovation Interdisciplinary Inorganic Chemistry Laboratory" $3,000 July 1990 5. U.S. Department of Education, MSIP Program "Improvement of the Inorganic Chemistry Laboratory"

$45,000 (Dr. Mayra Cadiz, P.I) March 1992-1994 6. MRCE(NSF)-UPR Outreach Program "Science on Wheels" (Dr. Juan Gonzalez Lagoa, Director) $30,000. September 1992-1994 7. Council Undergraduate Research, Fellowship to Zuleica Carrero $ 3,300 Sum. 1995 8. Howard Hughes Medical Institute. ”Experimental Science Education" Advancement in Biomedical Science Education" $ 100,000 (Dr. J. Gonzalez Lagoa, P.I) July 1991-1995 9. ASA-UPR-CEP Outreach Program "Science on Wheels" $45,000 (Dr. Juan Gonzalez Lagoa, P.I.) January 1994-June 1998 10. SA Minority University Research Centers "Science and Communications on Wheels" $ 300,081 (Dr. Rafael Fernandez Sein, P.I) July 1995- 2000 11. HICREST Undergraduate Research, (two students) $10,000 August 1999-2000 12. Department of Education and University of Puerto Rico. First to Eight GLOBE Workshop in Puerto Rico

$ 560,000 July 1997- October 2005 13. Pfizer Inc. Science on Wheels Demonstrations. $ 431,000 January 1997-Dec 2006 14 NSF Human Resources Development: Graduate and Undergraduate Students Enhancing Science and

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Technology in K-12 Schools (GUEST K-12. (P.I J. López -Garriga in collaboration with Dr. D. E. Alston, Dr. J. Santos, and Dr. J. Cortes) $1,660,000 March 2001-04 15 Howard Hughes Undergraduate Biological Education Program. Teachers Enhancement in Science and Technology: Bioscience Project $151,400 July 2001-2005 16 NASA Minority University Research Centers "GLOBE and Science on Wheels" $ 558,740 (Dr. Rafael Fernandez Sein, P.I). July 2000- 2005 17. Cordis, Inc. Puerto Rico, GLOBE program, $ 30,000 July 2005-2007 18. Pfizer-Puerto Rico. Science on Wheels and Schools Support. $ 34,000 May-2007-08 19. NSF Human Resources Development: Graduate and Undergraduate Students Enhancing Science and Technology in K-12 Schools (GUEST K-12. (P.I Juan López -Garriga with Dr. D. Alston, Dr. J. Santos, and Dr. J. Cortes) $2,160,000 March 2004- July 09 20. NSF Human Resources Development GK-12: International Collaboration in K-12 education between Puerto Rico and Spain. $86,000 December 2007-July 2009. 21. U.S. DEPARTMENT OF EDUCATION COLLEGE ACCESS CHALLENGE GRANT PROGRAM (CACGP) “Activities and Services for Increased Access to Postsecondary Education in Puerto Rico” P.I. José L. Cruz Rivera, June 2008 to December 2010. Total ~ $7,159,281. Funding for individual project $860,000

Graduate Students National/International Presentations in Technical Meetings (Total 80) 1. "Relation Between Narcosis and Mitochondria: Spectroscopic Studies" J. Hernández and J.L. Garriga. 1991 NIGMS Symp. Washington DC. November 1991 2. "Spectroscopic Studies of Cytochrome Oxidase in Mitochondria Membrane" Raul López and Juan L. Garriga, 1991 NIGMS Symp. Washington DC. November 1991 3. "Different Approaches to the Synthesis of Protonated Schiff's Bases Derivatives" J. Lastra and J. López-Garriga, XX-Latin American Chemical Cong., Santo Domingo, May 1992 4. "Interaction of N2O and N2 With Protein Models: Spectroscopic Studies" J. Hernandez and J. López -Garriga, XX-Latin American Chemical Congress, Santo Domingo May 1992 5. "Cytochrome Oxidase-CN in Mitochondria: Time Resolved Infrared" R. López, and J. López -Garriga, XX-Latin American Chemical Congress,Santo Domingo May 1992 6. "Interaction of N2O with Bovine Heart Mitochondria: Spectroscopic Studies" J. Hernandez and J. López -Garriga, MARC/MBRS Symposium, Dorado, P.R. October 1992 7. "Spectroscopic Studies of Protonated Schiff's Base Synthesis" J. Lastra, J.J. López-Garriga, MARC/MBRS Symposium, Dorado, Puerto Rico October 1992 8. "Time Resolved Infrared Spectroscopy: Application to Cytochrome c Oxidase", R. López, and J. López -Garriga, MARC/MBRS Symposium, Dorado, Puerto Rico. October 1992 9. "Cytochrome c Oxidase- Ligand Complex in Inner Mitochondrial: TRIR Studies" R. López -Mejias and J. López -Garriga ASBMB/DBC-ACS Meeting, San Diego, CA May 1993 10. "Outreach Chemistry Education on Wheels" Y. Echevarria- López, J. López -Garriga, Chem. Ed 93, Indianapolis, Indiana. August 1993 11. "Dynamic Interaction Between Nitric Oxide and Mitochondria: Time-Resolved Infrared" A.G. Irrizarry, J.J. López -Garriga, 206th ACS Nat. Meeting, Chicago,IL. August 1993 12. "Ligand Interactions Cytochrome Oxidase From Shark" A. Quintana*, C. Ramos J. López-Garriga, NIGMS-MBRS/MARC Symposium, Atlanta, GeorgiaNovember 1993 13. "Isolation of Halophilic Bacteria from Cabo Rojo, Puerto Rico". R. Montalvo and J. López-Garriga, 93th Meet. American Society for Microbiology. Las Vegas, Nevada, May 1994. 14. "Science on Wheels: An Outreach Program" Y. Echevarria and J. Lopez-Garriga 13th International Chemical Education Conf." San Juan P.R. August 1994 15. "Mitochondrial Inner Membrane Interaction with Nitric Oxide: Spectroscopic Studies" A.G. Irrizarry and J.J. López -Garriga, 208th ACS National Meeting, Washington. August 1994 16. "1NMR Studies of Hemoglobin I from Lucina Pectinata. A. Navarro, M. Maldonado, J. Colon and J.J. López -Garriga. 208th ACS National Meeting, Washington. August 1994 17. "Electron Transfer Activity in Monomeric and dimeric Inner Membrane Cytochrome C Oxidase and

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Cytochrome C." J.F. Cerda, C. Ramos , J. Lopez-Garriga, NIGMS-MBRS/MARC Symposium, Hilton Head Island, South Caroline, December 1994 18. "Methylneimine and Methyleneiminium Ions as Model Compounds to Study Schiff's Bases Behavior by Ab-initio Methods". A. Cuello, J.J. López -Garriga, 209th ACS National Meeting, Anaheim, CA. April 1995. 19. "VHOC from Natural Environments" A. Quintana, J.J. López -Garriga, 209th ACS National Meeting, Anaheim, CA April 1995 20. “A Comparison of Spectroscopic Properties Between Monomeric and Dimeric Cytochrome Oxidase “A. Cerda, C. Ramos, J.L.Garriga MRCE Meeting, Nashville, March 1996 21. “Taxonomic Studies of Archaebacteria” R. Montalvo, J. López -Garriga MRCE Meeting, Nashville, Tennessee. March 1996 22. “Synthesis and Characterization of Soluble Tricarbonyl Polypyridyl Rhenium (I) Complexes used for Lucina pectinata HbI Modification”. R. Alarcon, J.L. Colon, J. López -Garriga 211th ACS National Meeting, New Orleans . USA . March 1996 23. “1H NMR Study of Met-Cyano-HbI Complex of Lucina pectinata E. Silfa,, J. Colon , J. López -Garriga. XVII th Magnetic Resonance in Biological Systems. Colorado August 1996 24. “1H NMR Spectrum Assignment of the Heme Pocket of HbI-CO Complex from Lucina pectinata” M. Almeida, S. Wu, J. Colon, J. López -Garriga. XVII th International Conference in Magnetic Resonance in Biological Systems. Keystone, Colorado. August 1996 25. “1H NMR Spectrum Assignment of the Heme Pocket of HbI-CO Complex from Lucina pectinata” M. Almeida, J. López -Garriga. MRCE Meeting, El Paso, Texas. March 1997 26. “Resonance Raman Studies of Heme-Ligand Complexes from Lucina pectinata. J. Cerda, J. López -Garriga MRCE Meeting, El Paso, Texas. March 1997 27. “Resonance Raman Studies of the HbI-CO from Lucina pectinata. E.Morales, J. Cerda,J. López -Garriga 214th ACS Nat. Meet. Las Vegas, Nevada. Septembe 1997 28. “1H NMR Study of Hemoglobin I from Lucina pectinata. E. Silfa, M. Almeida, S. Wu, J. López -Garriga 214th ACS Nat. Meet. Las Vegas, Nevada.September 1997 29. Dynamics of HbICO conformers from Lucina pectinata. C. Ramos, J. López -Garriga17th IUPAC Symposium on Photochemistry. Sitges (Barcelona, Spain) p376, July 1998 30. Ultrafast Absorption Measurements of HbICO Complex from . pectinata C. Ruiz, R. Pietri, J. López -Garriga 17th IUPAC Symp. on Photochemistry Sitges (Spain) p376, July 1998 31. Science Concepts and Technology: A coherent Link Between Chemistry Activities and GLOBE. De Jesus, J. López -Garriga, R. Camacho, W. Nazario, D. Mora, M. Camacho, and J. Fuentes. 15th Biennial Conference on Chemical Education, Waterloo, Canada August 1998 32. Separation of Lucina pectinata Hemoglobins using Free Flow Electrophores. Zuleica Sanoguet, J.J. López -Garriga 216th ACS Nat. Meet. Boston, MA . August 1998 33.”Isolation of RNA from Lucina pectinata” F.M. Antonmattei, T. Rosado, C. Cadilla, J. López-Garriga 216th ACS Nat. Meet. Boston, MA . August 1998 34. cDNA Cloning and Characterization of Hemoglobin I from Lucina pectinata. F.M. Antonmattei, T. Rosado, V. Collazo, C. Cadilla, J. López -Garriga FASEB, J. 1356, May 1999 35. Investigation of the Hemoglobin I Carbonmonoxide and Oxy Complexes from Lucina pectinata by Femtosecond transient Absorption. C. Ramos, E. Ramirez, R. Pietri, C. Ruiz, J. Helbing, M. Chergui, J. Lòpez-Garriga Vth Femtochemistry Conf., Spain. September 2001 36. “GUEST K-12: An initiative to Improve K-12 Science Education L.Torres, A. Seda, R. Camacho and J. López-Garriga 224th ACS National Meeting Boston, MA August 2002 37. “CBL-2 Ti83 Plus and Probes in K-12 Teachers Development. D.Perez, M. Lugo, R. Camacho, J. López-Garriga* 224th ACS National Meeting Boston, M. August 2002 38. cDNA-Derived Amino Acid Sequence for Hb II from Lucina pectinata. Torres-Mercado, E., J. López-Garriga, C. Cadilla-Vazquez. ACS New Orleans. April 2003 39. Distal heme pocket interactions and analysis of cysteine sites in hemoglobins cyanide complexes from L. pectinata. J.Ramos, J. López Garriga ACS New Orleans April 2003 40. Ligand stabilization in the cyanide hemoglobin (I, II, and II) complexes from Lucina pectinata Anthony Cruz Balberdi, J. López Garriga. ACS New Orleans. April 2003

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41. Transformation of the Teaching Methodology in a Laboratory Course of the Industrial Biotechnology Curriculum in Response to the Student Profile. L. Saliceti, R. Pietri, R. Buxeda, J. Lopez-Garriga International Conference on Engineering Education, Valencia. Spain. July 2003 42. Ultrafast Studies of Ferrous and Ferric Complexes with Hemoglobin I from Lucina pectinata E. Ramírez, C. A. Ramos, R. Pietri, M. M. Rodríguez, J. López-Garriga, M.Chergui. Femtochemistry VI, Paris, July 2003. 43. Kinetic studies expressed HbISH 2 complexes. A. Del Toro, E. Collazo, R. León, J. López-Garriga. XIIIth International Conference on Invertebrates Dioxigen Binding Proteins, Mainz, Germany. September 2003 44. Distal contacts strength of the cyanide hemoglobin complexes from Lucina pectinata A. Cruz, J. Ramos, L. Torres, R. Pietri, H. Arbelo, J. López-Garriga, XIIIth International Conference on Invertebrates Dioxigen Binding Proteins, Mainz, Germany. September 2003. 45. Tyrosine B10 and heme interactions in the HbII from L. pectinata W. De Jesús Bonilla, A. Cruz Balberdi, E. Torres Mercado, A. Lewis Ballester, R.G. León, C. Cadilla, L. Kiger, J. López Garriga. XIIIth International Conference on Invertebrates Dioxigen Binding Proteins, Mainz, Germany. September 2003 46. Oxygen and CO ligand kinetics of Mutated HbI from L. pectinata. R. Gretchen, L. Kiger, M. Marden, C. Cadilla, J. López-Garriga. XIIIth International Conference on Invertebrates Dioxigen Binding Proteins, Mainz, Germany. September 2003. 47. Ligand biding kinetics of recombinant wild type and several mutants of HbI from L. pectinata. R. Gretchen, L. Kiger, M. Marden, C. Cadilla, J. López-Garriga 55th SERM-ACS 2003, Atlanta. November 2003 48. Expression and ligand binding studies of recombinant and mutated forms of HbI, 8th IUBMB Conference and ASBMB Annual Meeting, Boston, MA. Travel award winner. June 2004 49. Graphic Calculator and Calculator Based Laboratory (CBL) Technology: A tool for promoting interest in Pre-college Chemistry. Leslie V. Villalobos, Juan López-Garriga 56th Southeastern Meeting of the American Chemical Society (SERMACS), Research Triangle Park, NC. November 2004 50. Graduate Chemistry Students as Mentors in k-12 Science Education using GLOBE. R. Camacho, López-Garriga. 56th Southeastern SERMACS, Research Triangle Park, NC. November 2004 51. Determination of the Open and Close Conformations of the of the Hemoglobin II from Lucina Pectinata by Ft-Ir. Laura B. Granell, Ruth Pietri, Ariel Lewis, Carmen Cadilla, Juan López-Garriga 56th Southeastern Meeting of the American Chemical Society (SERMACS), Research Triangle Park, NC. November 2004 52. Resonance Raman Studies of the Heme-Ligand Active Site of HbI and HbII.Ariel Lewis, Héctor Arbelo, Ruth G. León, Carmen L. Cadilla, Juan López-Garriga , David Li, Syun-Ru Yeh56th Southeastern Meeting of the American Chemical Society (SERMACS), Research Triangle Park, NC. November 2004 53. Rodríguez Guilbe, M. López Garriga, J. Role of B10 position in HbI and HbII from Lucina pectinata by Femtosecond ultrafast studies. 229th ACS National Meeting, San Diego CA, February 2005 54. Brenda J., Gerd N La Mar, Carmen L. Cadilla, and Juan López-Garriga. Factors influencing the stability of the HbI F29Y CN moiety. 231st ACS National Meeting in Hemes and Heme Proteins, Atlanta. March 2006 55. Brenda J. Ramos, Gerd N La Mar, Carmen L. Cadilla, and Juan López-Garriga. Factors influencing the stability of the HbI F29Y CN moiety. 231st ACS National Meeting Inorganic Science Mix. Atlanta. March 2006 56. Carlos R. Ruiz-Martínez, Carlos A. Nieves-Marrero, Aliana López de Victoria-Suárez, Ingrid M. Montes-Rodríguez, Luis A. González, Juan M. García-Ruiz, and Juan López-Garriga. A crystallization study of the hemoglobins from Lucina pectinata. 231st ACS National Meeting Bioinorganic section. Atlanta. March 2006 57. Carlos R. Ruiz-Martínez, Carlos A. Nieves-Marrero, Aliana López de Victoria-Suárez, Ingrid M. Montes-Rodríguez, Luis A. González, Juan M. García-Ruiz, and Juan López-Garriga. A crystallization study of the hemoglobins from Lucina pectinata. 231st ACS National Meeting Inorganic Science Mix. Atlanta. March 2006 58. S. Mercado, J. López-Garriga. Expository programs: A new approach to enhancing chemistry and technology education. 231st ACS National Meeting. Atlanta. March 2006. 59. Granell, L.,Pietri, R., Leon,R., C.Cadilla, J. Lopez-Garriga. Effect of the distal pocket amino acids on the ligand binding in HbI from Lucina pectinata. 231st ACS National Meeting, Atlanta. March 2006 60. C. R. Ruiz-Martínez, C. A. Nieves-Marrero, J. M. García-Ruiz, J. López-Garriga. A crystallization study of the hemoglobins L. pectinata. International School on Biological Crystallization. Granada, Spain. May 2006 61. Cacimar Ramos- Michael Negrieri, Jean-Louis Martin, J. Lopez-Garriga, Ultrafast kinetics of the HbI and HbI mutants from L. pectinata.33RD Meeting American Society for Photobiology. Rio Grande, P.R. July 2006

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62. De Jesus, W. Y. Jia, A. Alayash and J. López Garriga Redox chemistry of the Hb1 and HbII hemoglobins from the calm Lucina pectinata. XIV International Dioxygen Binding and Sensing Proteins. Stazzione Zoologica Anton Dohrn, Naples (Italy) September, 2006 63. L. Granell, R. Pietri, R. León, C. Cadilla,J. López Garriga. Distal heme pocket amino acids: functional and structural factors in carbonmonoxide binding of Hemoglobin I from L. pectinata. XIV International Dioxygen Binding and Sensing Proteins. Stazzione Zoologica Anton Dohrn, Naples (Italy) September, 2006 64. Lopez-Garriga , J. J. Gavira, W. de Jesús, A. Camara-Artigas and J. García Ruiz. Crystallization and molecular structure of Haemoglobin II from L. pectinata. XIV International Dioxygen Binding Proteins. Stazzione Zoologica Anton Dohrn, Naples (Italy) September, 2006 65. L. López Cabán, M. Negrerie, J. Louis Martin and J. López Garriga. Ultrafast kinetics studies of HbI and HbI mutants from Lucina pectinata. XIV International Dioxygen Binding and Sensing Proteins. Stazzione Zoologica Anton Dohrn, Naples (Italy) September, 2006 66. Ramos B., V. Bondarenko, G. La Mar and J. López. Inside of HbI PheB10Tyr substation the structure and function of HbII from L. pectinata. XIV International Dioxygen Binding and Proteins. Stazzione Zoologica Anton Dohrn, Naples (Italy) September, 2006 67. Linda Rivera Rivera, L., López-Garriga, J., Cadilla, C.L. Characterization of Lucina pectinata Hemoglobin III Gene and cDNA-Derived Amino Acid Sequence. XIV International Conference on Dioxygen Binding and Sensing Proteins. Napole, Italy September, 2006 68. Elddie Marie Roman-Morales Juan Lopez Garriga Important HISE7 in the formation of sulfhemeproteins 233rd National ACS, Chicago, USA, March 2007 69. Samirah Mercado, Lopez-Garriga Juan, Expository programs: A model to influence learning in Chemistry. 41th IUPAC Congress , Turin, Italy, August 2007 70. Román-Morales, Elddie M., Lopez-Garriga,Important His E7 for the Formation of Sulfhemeproteins. 41th IUPAC Congress , Turin, Italy, August 2007 71. Débora Acevedo, Ivelisse Padilla, Samirah Mercado, J. López-Garriga, Chemistry outreach programs: Chemical education impact on teacher assistants. 235th ACS National Meeting, New Orleans, LA. Special Poster selected for the International presentation April 2008. 72. Samirah Mercado, J. López-Garriga, Expository programs: A model to improve teaching and learning of chemistry. 235th ACS National Meeting, New Orleans, LA. April 2008. 73. S. Mercado, J. Lopez-Garriga GLOBE: A model to improve teaching and learning of chemistry, 12th Annual GLOBE Conference in Cape Town, South Africa, June 2008. . 74. Pietri, R., Cadilla, C.L., Lopez-Garriga, J., Factors controlling the reactivity of hydrogen suldie with hemeproteins. XVth International Conference on Oxygen Binding and Sensing Proteins. University of Aarhus, Denmark, August 2008. 75. S. Mercado, Expository Programs: An Approach to Enhance the GLOBE Program 12th Annual GLOBE Conference in Cape Town, South Africa, June 2008. 76. Carlos Nieves, Carlos Ruiz, Rafael Estremera, J. Lopez-Garriga. Intenational Puerto Rico-Spain STEM GK12 education. Annual NSF GK-12 conference-Washington, March 2009 77. Carlos Ruiz, Carlos Nieves, Rafael Estremera, J. Lopez-Garriga. The GK-12 program in Puerto Rico. Annual NSF GK-12 conference-Washington, March 2009 78. Rafael Estremera, Carlos Nieves, Carlos Ruiz, J. Lopez-Garriga. Crystallization: A model to link Research and education in K-12. Annual NSF GK-12-Washington, March 2009 79. Ruiz, C., Nieves, C., Estremera,R., Gavira, J., Garcia-Ruiz, J., Lopez-Garriga, J. New crystallographic structures of the oxygen carrier hemoglobins from L. pectinata: Their functional interpretation. XVIth International Conference on Oxygen Binding and Sensing Proteins, Antwerp, Belgium. August 2010 80. Nieves, C., Ruiz, C., Estremera,R., Gavira, J., Garcia-Ruiz, J., Lopez-Garriga, J. Crystallographic studies of hemoglobin II from L. pectinata in the pH range from 9 to 4. XVIth International Conference on Oxygen Binding and Sensing Proteins, Antwerp, Belgium. August 2010

Graduate Students Presentations in Technical Meetings in Puerto Rico (Total 153) 1. "FTIR Spectroscopic Studies of the Relationship Between Narcosis and Ligand Binding to

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Heme-Proteins" J. Hernandez-Baez and J.J. López-Garriga, 25th ACS-PR Junior Technical Meeting, San German, P.R. March 1990 2. "Vibrational Studies on the CO-Heme Discrimination Processes" J. Lastra-Hernandez and J.J. López-Garriga, 25th ACS-PR Junior Tech. Meeting, San German, P.R. March 1990 3. "Time-Resolved Infrared: A Novel Spectroscopic Technique" R. López-Mejía, J.J. López-Garriga, 25th ACS-PR Junior Technical Meeting, San German, P.R. March 1990 4. "Time-Resolved Infrared: Spectroscopic Studies of Mitochondrial Complexes", R. López and J.J. López-Garriga 7th Student Forum for Research UPR., P.R. March 1991 5. "Kinetics Studies of Cytochrome c/Cytochrome Oxidase in the Presence of N2O" J. Hernandez and J.J. López-Garriga 26th ACS-PR Junior Meeting, ,UPR , P.R. March 1991. 6. "Molecular Basis for the C=N Frequency Shift Upon Protonation of Schiff's Bases" J. Lastra and J.J. López-Garriga, 26th ACS-PR Junior Tech. Meet.,UPR, Cayey, P.R. March 1991 7. "DMS Determination and Its Environmental Effects" Anibal Quintana and Juan L. Garriga. 27th. ACS Junior Meet. P.R. March 1992. 8. "TR Infrared Spectroscopy and Heme-Ligand Complexes" R. López -Mejias and J. López-Garriga, Marine Products: Chemical Structure and Bioactivity. Pargera P.R. March 1993 9. "Interaction of Cytochrome C Oxidase with Nitrous Oxide" J. Hernandez, A. Cuello and López-Garriga, Marine Natural Products. Pargera P.R. March 1993 10. "Kinetics Studies in Hemoglobin with CO Ligand" A. Irrizarry, R. López, A. Navarro, J. López-Garriga, 9th Student Forum, School of Medicine,University of Puerto Rico. April 1993 11. "Science on Wheels: An Outreach Program" Y. Echevarria- López, , V. Torres and J. López-Garriga, 17th ACS Senior Tech. Meet. Palmas del Mar, Puerto Rico. November 1993 12. "Ligand Interaction with Dimeric and Monomeric" A.G. Irrizarry and J. López-Garriga. 17th ACS Senior Tech.. Palmas del Mar, P.R. November 1993 13. "Lucina pectinata: Hemoglobin-CO Complex" A. Navarro, N. Rodriguez and J. López-Garriga. 17th ACS Senior Tech. Meet. Palmas del Mar, P.R. November 1993 14. "Characterization of Halobacterium Species from Solar Salters at Cabo Rojo, P.R.". R. Montalvo and J. L. Garriga, 29th Junior ACS Tech. Meet. Mayagüez, P.R. March 1994 15. "Interactions of Nitric Oxide with Cytochrome c Oxidase" A.G.Irizarry-Zambrana and J. López-Garriga 29th Junior ACS Tech. Meet. Mayagüez, P.R. March 1994 16. "FPLC Purification of Lucina pectinata Hemoglobin I" M. Maldonado, A. Navarro, J. López-Garriga and J. Colon 29th Junior ACS Tech. Meet. Mayagüez, P.R. March 1994 17. "A method for the Determination of VHOC in Marine Macroalgae" C. Suarez, A. Quintana, J. López -Garriga, 18th ACS Senior Meet. Aguadilla, P.R. November 1994 18. "Studies of mitochondrial Inner Membrane: Interaction with Ligands" A.G. Irrizarry and J. López -Garriga. 18th ACS Senior Tech. Meet. Aguadilla, P.RNovember 1994 19. "FPLC and Stopped Flow Measurements of Lucina pectinata HB I" R.G. Leon, A. Navarro, J. López-Garriga, J. Colon. 18th ACS Senior Tech. Meet. Aguadilla, P.R. Nov. 1994 20. "Spectroscopic Data and Ab-initio Calculations upon Protonation of Schiff's bases" A. Cuello and J. López-Garriga. 18th ACS Senior Tech. Meet. Aguadilla, P.R. November 1994 21. Time-resolved Infrared Spectroscopy: Hemoglobin Carbon Monoxide Dynamics". R. López and J. López-Garriga 18th ACS Senior Tech. Meet. Aguadilla, P.R. November 1994 22. "Spectroscopic Studies Lucina Pectinata's Hemoglobin I . A. Navarro, M. Vera, J. Colon, and J.J. López-Garriga. 18th ACS Senior Meet. Aguadilla, P.R. November 1994 23. "Hydrogen Bond in Carbon Monoxide Lucina pectinata Hemoglobin I" R.G. Leon, A. Navarro, J. López -Garriga and J. Colon. 30th ACS Junior Tech. Meet. Ponce, P.R. March 1995 24. Stopped-Flow Studies of Lucina pectinata HbI Redox Reactions R.G. Leon, A. Navarro, J. López-Garriga and J. Colon. 30th ACS Junior Tech. Meet. Ponce, P.R. March 1995 25. Molecular Modeling of Lucina pectinata HbI Maldonado, M.; J. Lopez-Garriga and J. Colon 30th ACS Junior Tech. Meet. Ponce, P.R. March 1995 26."Studies of Monomeric and Dimeric Cytochrome Oxidase" J.Cerda, Z.Sanoguet C. Ramos, A. Irizarry, J. López-Garriga, 19th ACS Senior Meet. Lajas, P.R. Nov ember 1995 27. "Ligand Complexes of Hemoglobin I from Lucina pectinata" M. Almeida, E. Silfa, Y. Echevarria, J. Colon,

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J. López -Garriga, 19th ACS Senior Meet. Lajas, P.R. November 1995 28. Heterogeneous Photocatalyst of VHOC using a Photosensitized Catalytic. J. Cajicas, J. López -Garriga. 31st ACS Junior Tech. Meet. Arecibo. P.R. March 1996 29. 1H NMR Study of Lucina pectinata Met-Cyano-Hemoglobin I Complex. E. Silfa, S. Wu, J. Colon , J. López -Garriga. 31st ACS Junior Tech. Meet. Arecibo. P.R. March 1996 30. 1H NMR of the Heme Pocket of HbI-CO complex from Lucina pectinata. M. Almeida, S. Wu, J. Colon, J. López-Garriga. 31st ACS Junior Tech. Meet. Arecibo. P.R. March 1996. 31. Resonance Raman Studies of AquoHbI from Lucina pectinata. Y. Echevarria, J. Cerda, J. Colon , J. López -Garriga. 31st ACS Junior Tech. Meet. Arecibo. P.R. March 1996 32. Modification of Lucina pectinata HbI with a Luminescence Ruthenium Bipyridyl Complex J. Colon , J. López -Garriga. 31st ACS Junior Tech. Meet. Arecibo. P.R March 1996 33. 1H NMR Study of Met-Cyano-HbI Complex of Lucina pectinata E. Silfa, S. Wu, J. Colon J. López -Garriga. 20th ACS Senior Meet. San Juan P.R November 1996 34. 1H NMR Assignment of the Heme Pocket of HbI-CO Complex from Lucina pectinata M. Almeida, J. López -Garriga. 20th ACS Senior Meet. San Juan PR November 1996 35. Photocatalyst of VHOC using a TiO2 Photosensitized by Phthalocyanines J. Cajicas, J. López -Garriga. 20th ACS Senior Meet. San Juan P.R. November 1996 36. Determination of VHOC and Fluoro Derivatives from Natural Environments. D. Acosta, B. Cruz, J. López -Garriga. 20th ACS Senior Meet. San Juan P.R. November 1996 37. Resonance Raman Studies of HbI from Lucina pectinata Y. Echevarria, , J. Cerda, J. Colon J.Lopez-Garriga. 20th ACS Senior Meet. San Juan P.R. November 1996 38. 1H NMR Study of HbI Complexes of Lucina pectinata E. Silfa,, J. López -Garriga. 21 th ACS Senior Meet. Lajas, P.R. November 1997 39. Biodegration of Organophosporous Compounds by extremely Halophilic Bacteria Celia Quinones, J.J. López -Garriga 21 th ACS Senior Meet. Lajas, November 1997 40. Determination of VHOC in Tropical Marine Systems by SPTD-SM and GC-MS. Brenda Cruz, J.J. López -Garriga 21 th ACS Senior Meet. Lajas, P.R November 1997 41. Separation of Lucina pectinata Hemoglobins using Free Flow Electrophoresis Zuleica Sanoguet, J.J. López -Garriga 21 th ACS Senior Meet. Lajas, P.R. November 1997 42. Resonance Raman Studies of HbI Lucina pectinata J. Cerda, J.J. López-Garriga 21 th ACS Senior Meet. Lajas, P.R. November 1997 43. Femtochemistry Laser Assembly for Applications in Ultrafast Resolved Spectroscopy R. Pietri, K. Rodriguez, C. Ruiz, J.López -Garriga 21 th ACS Sen. Meet. Lajas, P.R.Nov. 1997 44. Advance Photocatalytic Systems: Sensitization of Metal. Oxides. Eric Acosta, J. Cajigas, J.J. Lopez-Garriga 21 th ACS Senior Meet. Lajas, P.R. November 1997 45. Crystallization of 1-Br, 2-napthhyliderne-methylamine and its protonated derivatives. Patricia Osorio, J.J. López -Garriga 21 th ACS Senior Meet. Lajas, P.R. November 1997 46. Determination of VHOC and DMS in Open Sea and Bioluminescence Bays Tropical Environments D. Acosta, J. López -Garriga 21th ACS Senior Meet. Lajas,P.R. November 1997 47. Resonance Raman Studies of HbICO Isotopic Complexes of Lucina pectinata Erick Morales, Y. Ortiz, J. López -Garriga 21 th ACS Senior Meet. Lajas, P.R. November 1997 48. Separation of Lucina pectinata Hemoglobins using Free Flow Electrophoresis. Zuleica Sanoguet, J. López -Garriga 33st ACS Junior Tech. Meet. Bayamon. P.R March 1988 49. "Biodegradation of VHOC using new extremely halophilic bacteria." M. Meléndez, Juan López-Garriga, 22th ACS-PR Senior Technical Meeting, Aguadilla, P.R. November 1998 50. Ultrafast absorption measurements of Hemoglobin I-CO complex of L. Pectinata. C. Ruiz, R. Pietri, J, López Garriga, 22th ACS-PR Senior Tech. Aguadilla, P.R. November 1998 51. Resonance Raman of Lucina Pectinata Hemoglobin I Ligand. E. Morales, Y. Ortiz, J. López-Garriga, 22th ACS-PR Senior Tech. Meeting, Aguadilla, P.R. November 1998 52. Advanced photocatytic systems: sensization of TIO2 by CU Phthalocyanine. Erick Acosta J. López-Garriga, 22th ACS-PR Senior Tech. Meeting, Aguadilla, P.R. November 1998 53. CDNA Cloning and Characterization of Hemoglobin I from Lucina Pectinata. F.Antonmmattei, T.Rosado, C.Cadilla, J. López-Garriga, 22th ACS-PR Senior Technical Meeting, Aguadilla, P.R. November 1998

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54. Isolation and Characterization of Carotenoids from New Halobacteria Species. A. Katiyar, J. López-Garriga, 22th ACS-PR Senior Tech. Meeting, Aguadilla, P.R November 1998. 55. Isolation and purification of Hemoglobins I, II and III from Lucina Pectinata Using Isoelectric Focusing.. Zuleika Sanoguet, J.López-Garriga, 22th ACS-PR Senior Technical Meeting, Aguadilla, P.R. November 1998 56. Science on Wheels (SOW): A Cultural Bridge Between Pre-college and University Education. R. Camacho, J. Fuentes, Z. Sanoguet, J. López-Garriga, 22th ACS-PR Technical, Aguadilla, P.R. Nov. 1998 57. GLOBE: Pre-college Education Through World Wide Learning. R. Camacho, W. de Jesús, W. Nazario, E. González and J. López-Garriga, 22th ACS-PR Senior Technical Meeting, Aguadilla, P.R. November 1998 58. cDNA Cloning and Characterization of Hb I from L. pectinata F. Antomnattei, T. Rosado, V. Collazo, J. López -Garriga. 34th ACS Junior Tech. Meet. UPRM. March 1999 59. Cloning of HbI cDNA from Lucina pectinata into a Prokaryote His-Tagged Expression Vector T. Rosado, F.M. Antonmattei, C. Cadilla, J. López -Garriga, 35th ACS Junior Tech. Meet. San Juan P.R. March 2000 60. Resonance Raman Studies of the HbI-ligand Complexes from Lucina pectinata A. Cruz, E. Morales, J. López -Garriga, 35th ACS Junior Tech. Meet. San Juan P.R. March 2000 61. Kinetic Studies of the Binding of Hydrogen Sulfide to Hemoglobin I (HB I) from Lucina pectinata Entrapped in Sol-Gel. M. Camacho-Zapata, L. Estrada,, J. I. Zink, J. López-Garriga, 31th Annual Senior Technical Meeting, Aguadilla, P.R. November 2000 62. Science on Wheels: Adding Excitement to Education in Puerto Rico. R. Camacho, M. Vélez, J. López-Garriga, 31th Annual Senior Technical Meeting, Aguadilla, P.R Nov. 2000 63. Resonance Raman Studies of Methydroxide Hemoglobin I Complex from Lucina Pectinata A. Cruz-Balberdi, J. López-Garriga, E. Moralez, 31th Annual Senior Technical Meeting, Aguadilla, P.R. November 2000 64. Reactions of Hemoglobin I L. Pectinata with Hydrogen Peroxide: Formation of HbI-Compound II Ferryl Species. W. de Jesus, J. López-Garriga, J. Cortés- 31th Annual Senior Technical Meeting, Aguadilla, Nov. 2000 65. Studies of Heme-Isotopic Complexes of Hemoglobin I from Lucina Pectinata A. Cruz, J. López-Garriga, E. Morales, 31th Senior Tech Meet,, Aguadilla, P. Nov. 2000 66. Evidence of Ultrafast Geminate Recombination of Cyanide with HemoglobinI L. pectinata. R. Pietri, C. Ruiz, J. Helbing, M. Chergui, J. López-Garriga, 31th Annual Senior Technical Meeting, Aguadilla, P.R. Nov 2000 67. Unexpected Ultrafast Signal Recombination of the Hemoglobin I Carbonmonoxide Complex from Lucina Pectinata R. Pietri, C. Ruiz, J. Helbing, M. Chergui, J. López-Garriga, 31th Annual Senior Technical Meeting, Aguadilla, P.R. November 2000 68. GLOBE-Al Science at Local Forums. M. Vélez, R. Camacho, M. Camacho, J. López-Garriga, 31th Annual Senior Technical Meeting, Aguadilla, P.R. November 2000 69. Evidence of Ultrafast Geminate Recombination of Cyanide with Hemoglobin I from L. pectinata R. Pietri, C. Ruiz, C. Ramos, J. López-Garriga 13th AAAS & Puerto Rico EPSCoR Meeting, Cerromar, P.R. April 2001 70. Formation of Compound I and Compound II Ferryl Species in the Reactions of Hemoglobin I from Lucina pectinata with Hydrogen Peroxide Walleska De Jesús, E. Ramírez, J. López-Garriga, 13th AAAS & P.R. EPSCoR Ann. Meeting, Cerromar, P.R. April 2001 71. Resonance Raman Studies of methydroxide Hemoglobin I complex of L. pectinata. A. Cruz, J. López-Garriga, 13th AAAS & P.R. EPSCoR Ann. Mett, Cerromar, P.R. April 2001 72. Unexpected Ultrafast Signal Recombination of the Hemoglobin I Carbonmoxide Complex from L. pectinata C. Ruiz, J. López-Garriga, 13th AAAS & EPSCoR Annual Meeting, Cerromar, P.R. April 2001 73. Resonance Raman Studies of HemoglobinII from Lucina pectinata. A. Cruz 60th Chemical Conference, Colegio de Quimicos Rio Grande, P.R. August 2001. 74. Science on Wheels Educational Center: An integration of Outreach Programs from Industry, Government, and Academia. R. Camacho, J. López -Garriga 14th EPSCoR Meeting, Dorado P.R. May 2002 75. Essential Development for the New Generation Oxygen Carrier Substitutes: The Cyclic Reaction of Hemoglobin I with Hydrogen Peroxide. J. López –Garriga, C. Ramos, R. Pietri, W. De Jesús, E. Ramírez, J. Cerda. 14th EPSCoR Meeting, Dorado P.R. May 2002 76. Large scale expression and purification of HbI recombinant protein.” E Collazo, J. López -Garriga ACS 26th Senior Tech. Meet. San Juan, P.R. August 2002 77. FTIR and RR studies of CNHbI and CNHbII complexes from L. pectinata. A.Cruz, J. Ramos, J. López -

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Garriga ACS 26th Senior Tech. Meet. San Juan, P.R. August 2002 78. NMR Investigation of the influence of distal Gln in HbI and HbII from L. pectinata. Ramos, B., J. López –Garriga. ACS 26th Senior Tech. Meet. San Juan, P.R. August 2002 79. The amino Acid sequence of HbII from L. pectinata. E. Torres-Mercado, J. López -Garriga ACS 26th Senior Tech. Meet. San Juan, P.R. August 2002 80. Protein Research Center: Multicampus effort to study protein structure, function, and dynamics. M. Vera, J. López –Garriga. ACS 26th Senior Tech. Meet. San Juan, P.R.Aug. 2002 81. Science on Wheels Educational Center: An integration of Outreach Programs in Scientific Societies” R. Camacho, J. López -Garriga ACS 26th Senior Tech. Meet. San Juan, P.R. August 2002 82. Study of the free electron pair behavior in the LiCN and LiCNH systems. L. Torres, J. López -Garriga, ACS 26th Senior Tech. Meet. San Juan, P.R. August 2002 83. RR studies of MetHbI and N3HbI complexes from L. pectinata. A. Cruz, C. Manrrique, J. López -Garriga ACS 26th Senior Tech. Meet. San Juan, P.R. August 2002 84. Ultrafast studies of the ferryl intermediate of HbI from L. pectinata and H2O2. E. Ramirez, C. Ramos, J. López -Garriga ACS 26th Senior Tech. Meet. San Juan, August 2002 85. Integration of CBL-2 Ti83 Plus and Probes in K-12 Teachers Development. D. Perez, M. Lugo, R. Camacho, J. López-Garriga. Latin American and Caribbean Biotechnology Congress, Mayagüez Resort and Casino, PR. November 2002 86. Educational Improvement in Puerto Rico by the GLOBE program. N. Rodriguez, E. Gomez, J. López -Garriga. Latin American and Caribbean Biotechnology Congress, Mayagüez Resort PR. November 2002 87. The CDNA derived amino Acid sequence of HbII from L. pectinata. E. Torres-Mercado, J. López -Garriga. Latin American and Caribbean Biotechnology Congress, Mayagüez P.R. November 2002 88. RR CNHbII complexes from L. pectinata. A.Cruz, J. López -Garriga Latin American and Caribbean Biotechnology Congress, Mayagüez Resort and Casino, PR. Nov. 2002 89. NMR Investigation of the influence of distal Gln on the structural properties of HbI and HbII from L. pectinata .B. Ramos-Santana, J. López -Garriga. Latin American and Caribbean Biotechnology Congress, Mayagüez Resort and Casino, PR. November 2002 90. Large scale purification of HbI recombinant protein.” E Collazo, J. López -Garriga Latin American and Caribbean Biotechnology Congress, Mayagüez. P.R. November 2002 91. Ultrafast studies of the ferryl intermediate in the reaction of HbI from L. pectinata and H2O2. E. Ramirez, C. Ramos, J. López -Garriga. Latin American and Caribbean Biotechnology Congress, Mayagüez Resort and Casino, PR. November 2002 92. Study of the free electron pair behavior in the LiCN and LiCNH systems. L. Torres, J. López -Garriga, Latin American and Caribbean Biotechnology Congress, Mayagüez Resort and Casino, PR. November 2002 93. Ultrafast Studies of Nitric Oxide Complexes with Hemoglobin I from Lucina Pectinata C. Ramos, R. Pietri, E. Ramírez M. Rodríguez, J. López-Garriga 1st Colloquium in Protein Structure, Function, and Dynamics (pag. 89-91) Ponce P.R. Feb 2003. 94. Studies of Heme-Ligand binding Stabilization in the HbI of Lucina Pectinata by Liquid State Nuclear Magnetic Resonance Spectroscopy B. Ramos-Santana, J. López Garriga. 1st Colloquium in Protein Structure, Function, and Dynamics (pag. 63-65) Ponce P.R. Feb. 2003 95. Distal Contacts in the Cyanide Hb complexes from L. pectinata J. Ramos, A. Cruz , L. Torres, J. López-Garriga 1st Colloquium in Protein Structure, Function, and Dynamics (pag. 19-21) , Ponce P.R. Feb. 2003 96. Functional Characterization of the Purified Holo Hemoglobin I from Lucina Pectinata over expressed in E. coli. E. Collazo R. Pietri, W. de Jesús, C. Cadilla, J. López-Garriga 1st Colloquium in Protein Structure, Function, and Dynamics (pag. 29-31) , Ponce P.R. Feb. 2003 97. Optimization of the Expression and Characterization of L. Pectinata Recombinant HbI in E. Coli. C. Cadilla, R. León, O. Barzu, H. Munier-Lehmann, J. López Garriga. 1st Colloquium in Protein Structure, Function, and Dynamics (pag. 42-44) Ponce P.R. Feb. 2003, 98. Pursing the hydrogen sulfide symbiotic bacteria inside Lucina pectinata, Nydia Irrizary, G. Nadathur,, J. López -Garriga ACS Junior Tech. Meet. March 2004. 99. High level expression and ligand binding kinetics of recombinant and mutated forms of HbI from L. pectinata. R.G. Leon, C. L. Cadilla, L. Kiger, J. López -Garriga. Spring Conference in Protein Research, COBRE II-NIH –UPR Center, San Juan. April 2004

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100. H2S symbiotic bacteria in L. pectinata. N. Irizarry, N. Govind, C. Rios., J. López -Garriga. Spring Conference in Protein Research, COBRE II-NIH –UPR Center, San Juan. April 2004 101. Resonance Ramanof the heme-ligand active site of Hb’s L. pectinata. A. Lewis, H. Arbelo, D. Li, Y. Syun-Ru, J. López -Garriga. COBRE II-NIH–UPR Center, San Juan. April 2004 102. Roles of HbII of L. pectinata in ultrafast events. M. M. Rodriguez-Guilbe, R. Pietri, C. Ramos, J. López -Garriga. COBRE II-NIH –UPR Center, San Juan. April 2004 103. The role of B10 position in the heme proteins in the reactivity of compound I and compound II. W. De Jesus-Bonilla, R. G. Leon, C. Cadilla, J. López -Garriga. COBRE II-NIH –UPR Center, San Juan. April 2004 104. The role of ultrafast events of hemoglobin I and hemoglobin II from L. Pectinata. M. Rodríguez, R. Pietri, C. Ramos, L. López, J. López-Garriga, XXVIII ACS Senior Technical Meeting, Isabela, P.R. November 2004 105. Tyrosine B10 and heme-ligand interactions of Lucina Pectinata hemoglobin II: control of hem reactivity. R. Pietri, L. Granell, A. Cruz, W. De Jesús, A. Lewis, R. León, C.L. Cadilla and J. López-Garriga, XXVIII ACS Senior Technical Meeting, Isabela, P.R. November 2004 106. The role of B10 position in heme proteins in the reactivity of compound I and compound II. W. De Jesús-Bonilla, C. Cadilla, J. López-Garriga, XXVIII ACS Senior Technical Meeting, Isabela, P.R.Nov. 2004 107. Graduate chemistry students as mentors in k-12 science education using GLOBE. R. Camacho-Zapata, E. Román, Y. López and J. López-Garriga, XXVIII ACS Senior Technical Meeting, Isabela, P.R. Nov. 2004 108. Resonance Raman Studies of the heme-ligand active site of hemoglobins from L. Pectinata. H. Arbelo, C. Cadilla, J. López-Garriga , D. Li, and S.-R. Yeh, XXVIII ACS Senior Meeting, Isabela, P.R. Nov. 2004 109. Stabilization mechanism of sulfide and cyano hemoglobin I complex from L. Pectinata. B.J. Ramos-Santana, D.E. Baceló, J. López-Garriga, XXVIII ACS Senior Technical Meeting, Isabela, P.R. November 2004 110. Pietri-Melendez, Ruth, López -Garriga, J. Flexibility of the distal heme pocket of Lucina pectinata probed by infrared spectroscopy. 2nd Colloquium in Protein Structure, Function and Dynamics Caribe Hilton, San Juan, Puerto Rico, February 2005 111. Ramos-Santana, Brenda, Gerd N. La Mar, Carmen Cadilla, Juan López Garriga. H NMR Structural characterization of Hemoglobin I F29Y mutant CN complex. 2nd Colloquium in Protein Structure, Function and Dynamics Caribe Hilton, San Juan, PR, February 2005 112. León, R. ,M. Marden, D. E. Bacelo, R.C. Binning Jr., C. L. Cadilla, J. López Garriga Ligand-binding of Recombinant and mutant forms of HbI from L. pectinata. 2nd Colloquium in Protein Structure, Function and Dynamics Caribe Hilton, San Juan, February 2005 113. De Jesús Bonilla, Walleska, Ariel Lewis, Carmen Cadilla, and Juan López Garriga Peroxidative reactions of Lucina pectinata hemoglobins. 2nd Colloquium in Protein Structure, Function and Dynamics Caribe Hilton, San Juan, Puerto Rico, February 2005 114. Granell-Ortiz, L., B., R., Pietri, R. León, C. Cadilla, J. López Garriga. Roles of the amino acid residues on positions E7 and B10 of Hemoglobin I from L. pectinata. 2nd Colloquium in Protein Structure, Function and Dynamics Caribe Hilton, San Juan, February 2005 115. Rodríguez Guilbe, María M., Ruth Pietri, Cacimar Ramos, Lourdes López, Juan López Garriga. The role of B10 position in Hemoglobin I and Hemoglobin II from L.pectinata by Femtosecond ultrafast studies. 2nd Colloquium in Protein Structure, Function and Dynamics Caribe Hilton, San Juan, Puerto Rico, February 2005. 116. Granell-Ortiz, Laura Benice, Ruth Pietri, Ariel Lewis, Ruth León, Carmen Cadilla, Juan López-Garriga, Spectroscopic evidence of the roles of the amino acid residues on positions E7 and B10 of Hemoglobin I from Lucina Pectinata. 25th Puerto Rico Interdisciplinary Scientific Meeting & 40th ACS Junior Technical Meeting, Mayagüez, Puerto Rico, March 2005 117. Expository Programs: A New Approach to Enhancing Chemistry and Technology Education. S. Mercado, R. Camacho, J. López Garriga. XXIX Senior Technical Meeting, Lajas, Puerto Rico, November 2005. 118. Determination of open and closed conforations of HbII from L. pectinata. L. B. Granell, R. Pietri, R. Leon, C. Cadilla, J. López Garriga. XXIX Senior Technical Meeting, Lajas, Puerto Rico, November 2005. 119. Implications of E7 and B10 residues in the affinity of H2S for HbI of L. pectinata. Y. López-Cuevas, R. Pietri, J. López-Garriga. XXIX Senior Tech. Meet., Lajas, PR, Nov. 2005. 120. A Cristalization Study of the Hemoglobins from L. pectinata. Ruiz-Martinez, C.; Nieves-Marrero, C.; López de Victoria, A.; Montes, I.; Gonzáles, L.; García-Ruiz, J.; López-Garriga, J. XXIX Senior Technical Meeting, Lajas, Puerto Rico, November 2005.

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121. Short time dynamics of HbI, HbII from L. Pectinata. C. Ramos, L. López, M.Rodríguez, R. Pietri, J. López-Garriga. XXIX Senior Tech. Meeting, Lajas, Puerto Rico, November 2005. 122. 1H NMR Structural characterization of HbI F29Y mutant from L. pectinata. B. Ramos, La Mar, G. N., C. Cadilla, J. López-Garriga. XXIX Senior Tech. Meet. Lajas, PR, Nov. 2005. 123. Effect of the distal pocket amino acids on the ligand binding in HbI. L. Granell, R. Pietry, R. Leon, C. Cadilla, Lopez-Garriga, J. COBREII_spring Conference. San Juan PR March 2006 124. The cDNA-Derived amino acid sequence of HbIII from L. pectinata. L. Rivera, Lopez-Garriga, J, Cadilla, C.L. COBREII_spring Conference. San Juan PR March 2006 125. Lorenzo, W., Cadilla, C.L., Lopez-Garriga, J., Bacterial Expression of Recombinant Hemoglobin II and the Site-directed Mutant HbII PheE11→Tyr from the Clam Lucina pectinataMBRS/RISE Summer Research Conference San Juan, Puerto Rico, August 2006 126. The Assessment of the Biological Stability and Activity Model Proteins Encapsulated in Ionic Hydrogel Membranes. C. Valentín-Rodríguez, C. Román-Cuevas, M. Torres-Lugo, J. López-Garriga. XXX Senior Technical Meeting “ Advances in Chemical Sciences and Engineering in Puerto Rico. Dorado P.R. Nov. 2006 127. Preliminary electrochemical analysis of the hemoglobin I from Lucina pectinata. M. Ortega Núñez, P. Báez Gutiérre, C. Vega Olivencia, J. López Garriga. XXX Senior Technical Meeting “ Advances in Chemical Sciences and Engineering in Puerto Rico. Dorado Nov. 2006 128. Implication of HbI PheB10Tyr substitution in the structure and function of HBII of L. pectinata. B. Ramos, G. La Mar, J. López-Garriga. XXX Senior Technical Meeting “ Advances in Chemical Sciences and Engineering in Puerto Rico. Dorado P.R. November 2006 129. Important HisE7 in the formation of sulhemeproteins. E. Román-Morales, J. López-Garriga. Advances in Chemical Sciences and Engineering in Puerto Rico. Dorado. Nov. 2006 130. Distal heme pocket amino acids: Functional and structural factors in carbonmonoxide binding of Hemoglobin I from Lucina pectinata. L. Granell, R. Pietri, R. León, C. Cadilla, J. López-Garriga. XXX Senior Technical Meeting “ Advances in Chemical Sciences and Engineering in Puerto Rico. Dorado P.R. Nov.2006 131. Ultrafast kinetics of HbI mutants from Lucina pectinata. C. Ramos, L. López, J. López Garriga, M. Negrerie, J. Louis Martin. XXX Senior Technical Meeting “ Advances in Chemical Sciences and Engineering in Puerto Rico. Dorado P.R. November 2006 132. Implications of the distal residues E7 and B10 in the Affinity of H2S and O2 ligands with the Hemoglobin I from Lucina pectinata. R. Pietri, J. López Garriga, R. Leon, C. Cadilla, L. Kiger, M. Marden. XXX Senior Technical Meeting “ Advances in Chemical Sciences and Engineering in Puerto Rico. Dorado P.R. Nov. 2006 133. Detection of hydroperoxy and compound I species in the oxidative reactions of myoglobin. H. Arbelo López, J. López-Garriga. XXX Senior Technical Meeting “ Advances in Chemical Sciences and Engineering in Puerto Rico. Dorado P.R. November 2006 134. The role of E11 in the HbISH2 complex formation in L. pectinata. Y. López-Cuevas, R. Pietri-Meléndez, J. López-Garriga. XXX Senior Technical Meeting “ Advances in Chemical Sciences and Engineering in Puerto Rico. Dorado P.R. November 2006 135. Elddie Marie Roman, J. Lopez-Garriga Important HisE7 in the formation of sulfhemeproteins. XXX Senior Technical Meeting “ Advances in Chemical Sciences and Engineering in Puerto Rico. Dorado P.R. Nov. 2006 136. Factors influencing the stability of the HbI F29Y CN moiety. Brenda J. Ramos, Gerd N La Mar, Carmen L. Cadilla, and Juan López-Garriga. 2nd UPR- Research interaction in Bioscience, Mayaguez, P.R. Feb. 2007 137. Carlos R. Ruiz-Martínez, Carlos A. Nieves-Marrero, Aliana López de Victoria-Suárez, Ingrid M. Montes-Rodríguez, Luis A. González, Juan M. García-Ruiz, and Juan López-Garriga. 2nd UPR- Research interaction in Bioscience, Mayaguez, P.R. February 2007 138. Laura B. Granell, Ruth Pietri, Ruth G. Leon, Carmen L. Cadilla, and Juan Lopez-Garriga. Effect of the distal pocket amino acids on the ligand binding in HbI from Lucina pectinata. 2nd UPR- Research interaction in Bioscience, Mayaguez, P.R. February 2007 139. Immobilization of Hemoglobin form 1 Lucina pectinata using two Different Supports on Glassy Carbon Electrochemical Analysis. Ortega, Mario E. Lopez-Garriga, J. XXXI Senior ACS Technical Meeting, Mayagüez Resort & Casino, Mayaguez P.R. November 2007 140. Oxidation Reactions of Lucina pectinata Hemoglobins: Hbl1 as a Model System to Design a Heme Protein based Oxygen Carrier. De Jesús-Bonilla, Waleska, Lopez-Garriga, J. . XXXI Senior ACS Technical Meeting, Mayagüez Resort & Casino, Mayaguez P.R. Nov. 2007

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141. The Role of Position E11 in the Stabilization of the Hbl-SH2. López-Cuevas, Yahira, Lopez-Garriga, J. XXXI Senior ACS Technical Meeting, Mayagüez Resort & Casino, Mayaguez P.R. Nov. 2007 142. Expression and Purification of Recombinant Hemoglobin 11 from L. pectinata. Lorenzo, Wilmarie, Lopez-Garriga, J.XXXI Senior ACS Technical Meeting, Mayagüez Resort & Casino, Mayaguez P.R. Nov. 2007 143. Structural Impacts of a PheB1OTyr Substitution on Protein Stability of HbI L. pectinata. Ramos, B., Lopez-Garriga, J. XXXI Senior ACS Technical Meeting, Mayagüez Resort & Casino, Mayaguez P.R. Nov. 2007 144. Detection of Hydroperoxy and Ferryl Species in the Oxidative Reactions of Myoglobin. Arbelo-López, Héctor D., Lopez-Garriga, J. XXXI Senior ACS Technical Meeting, Mayagüez Resort, P.R. Nov. 2007 145. Important His E7 for the Formation of Sulfhemeproteins. Román-Morales, Elddie M., Lopez-Garriga, J. XXXI Senior ACS Tech. Meeting, Mayagüez Resort , Mayaguez Nov. 2007 146. Implecations of the Distal Residues E7, E11 and B10 in the Reduction on Fell-H2S Iron Center of the Hemoglobin form Lucina pectinata. Pietri, Ruth, Lopez-Garriga, J. XXXI Senior ACS Technical Meeting, Mayagüez Resort & Casino, Mayaguez P.R. Nov. 2007 147. Ultrafast Kinetics of Hb1 Mutants form Lucina pectinata. Ramos, Cacimar, Lopez-Garriga, J. XXXI Senior ACS Tech. Meeting, Mayagüez Resort, Mayaguez P.R. Nov. 2007 148. Expository programs: A model to influence learning in Chemistry. Samirah Mercado, Lopez-Garriga , J. XXXI Senior ACS Tech. Meeting, Mayagüez Resort, Mayaguez Nov. 2007

149. Rivera Rivera, L., López-Garriga, J., Cadilla, C.L. Alternative Splicing in the 5’ and 3’UTR of HbIII mRNA from Lucina pectinata. XXVII Annual forum of Research and Education. San Juan, P.R December 2007 150. Lorenzo, W., Cadilla, C.L., Lopez-Garriga, J., Overexpression of Recombinant HbII from L. pectinata. 32nd Senior Technical Meeting ACS Rincon, Puerto Rico. November 2008 151. Pietri, R., López-Garriga, J., Cadilla, C.L. Factors controlling the reactivity of hydrogen sulfide with hemeproteins. 32nd Senior Technical Meeting ACS, Rincon, Puerto Rico. Nov. 2008 152. Ramos, C., Pietri, R., Granell, L., López-Garriga, J., Optimization of recombinant and mutant HbI from L. pectinata. 32nd Senior Technical Meeting ACS Rincon, P.R.. Nov. 2008 153. Ortega, M., Vega, C., Tremont, R.,Lopez-Garriga, J. Electrochemical analysis of H2S and HbI using a cystein modified gold electrode. SERMACS, Puerto Rico Convention Center, San Juan P.R. October 2009

Undergraduate Students Presentations National and International (Total 14) 1. "Molecular Models of the C=N Bond of Retinal in Rhodopsin and Bacteriorhodopsin" Y. Echevarria and Juan López -Garrriga NIGMS Symp. Washington DC. November 1991 2. "Identification of Volatile Organic Compounds Produced by Actinomycetes" M. Cortes and J. López -Garriga. ACS Annual Meet. Denver Co. March 1993 3. Time-resolved Infrared Studies of HbI CO Complex from Lucina pectinata C. Ramos, J. Colon, J. López -Garriga. 212th ACS National Meet., Orlando, Florida. August 1996 4. Science Shows: An Outreach Program" H. Ruiz, J. Esteves, W. de Jesus, J. Lopez-Garriga. 212th ACS National Meeting, Orlando, Florida. August 1996 5. "Science on Wheels: An Outreach Program" E. Morales, R. Camacho, W. de Jesus and Juan López -Garriga 14th International Chemical Education Conf." August 1996 6. cDNA Cloning and Characterization of HbI from L. pectinata. E. Torres, F. Antonmattei, C. Cadilla, J. Lopez-Garriga, Hicrest Research Awareness Albuquerque, NM February 2000 7. Resonance Raman Studies of the HbI-ligand Complexes from Lucina pectinata. A. Cruz, E. Morales, J. López -Garriga, Hicrest Research Awareness -Albuquerque, NM. Feb. 2000 8. Cloning and Expression of Unique H2S Binding Hemoglobin from L. Pectinata A. Roman, V. Collazo, C. Cadilla, J. López -Garriga, Biotechnology Met. Washington Sept. 2000 9. Reactivity of Hydrogen Sulfide with Hemeproteins incorporated in Sol-Gel Matrices. T.Bohec, E. Bosques, J. López-Garriga, AICHE annual Meeting 2005, Cincinnati Convention Center, Cincinnati, OH. Nov. 2005 10. Edelmira Bosques, Tatiana Bohec, and Juan López-Garriga, Hydrogen sulfide reactivity with hemeproteins encapsulated in sol-gel matrices. 231st ACS National Atlanta. March 2006 11. Ligand binding kinetics of Hemoglobin II and HbIITyrB10Phe from Lucina pectinata overexpressed in Escherichia coli. T.Bohec, G.Afanador. XIV International Dioxygen Binding and Sensing Proteins. Stazzione Zoologica Anton Dohrn, Naples (Italy) September, 2006

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12. Ligand binding kinetics of Hemoglobin II and HbIITyrB10Phe from Lucina pectinata overexpressed in Escherichia coli. Gustavo Afanador Annual Biomedical conference for minority students, California, Poster presentation Award in Biochemical Sciences. November 2006 13. Pietri, Frances, Nieves, Abner, Pietri, R., Lopez-Garriga, J., Study of the Reaction Between Hemoglobin I from the Clam Lucina pectinata and Hydrogen Sulfide.Annual Biomedical Research Conference for Minority Students (ABRCMS) Orlando, Florida, November 2008 14. Marchany-Rivera, D., Hernández-Mercado, R. M., Cruz, J., Román-Morales, E. M., Ramos, B. J., López-Garriga, J. Sulfhemoglobin: Factors Affecting its formation. 237th ACS National Meeting Undergraduate Poster Section. Salt Lake City, Ut. March 2009

Undergraduate Students Presentations in Local Technical Meetings (Total 87) 1. "Blue Copper Proteins: RES Spectra and ET" C. Ribs, J. Arroyo, J.J. López-Garriga, 25th ACS-PR Junior Technical Meeting, San German, P.R. March 1990 2. "Molecular Models of the C=N Bond of Retinal in Bacteriorhodopsin" Y. Echevarria and J.J. López-Garriga, 26th ACS-PR Junior Meeting, U.P.R. Cayey Campus P.R. March 1991 3. "Study of Bacteriorhodopsin in Halobacterium Halobium from Tropical Environments" Rafael Montalvo and J. López -Garriga, AMP Summer Res. Meet., RUM, P.R. July 1992 4. "Production of VHOC by Wood Decay Fungi in Tropical Forest" Jesus Salas and J. López -Garriga, AMP Undergraduate Summer Research Tech.Meet. RUM, UPR, July 1992 5. "Identification of Volatile Organic Compounds from Actinomycetes" Maria Cortes-Cobos and J. López-Garriga. AMP Summer Research Meeting, RUM, P.R. July 1992 6. "Extraction of Cytochrome C Oxidase from Shark Heart" V. Acosta and J. López -Garriga. 28th. ACS Junior Meet. P.R. March 1993 7. "Isolation of Halobacterium Halobium in Cabo Rojo, Puerto Rico". M. Mendez, R. Montalvo and J. López -Garriga 28th. ACS Junior Meet. P.R. March 1993 8. "Identification of Halobacterium Halobium in Salt Beds of Cabo Rojo, Puerto Rico, R. Montalvo, M. Mendez, and J. López -Garriga. 3rd RCMS Meeting, P.R. May 199 9. "Extraction of Mitochondrial Cytochrome c Oxidase from Shark Heart" C. Ramos, J. L. Garriga, AMP Undergraduate Summer Research Technical Meeting, RUM, UPR, July 1993 10. Purification of Hb from L. Pectinata. A. Navarro, López -Garriga 29thACS Mayagüez, P.R. March 1994. 11. "Mitochondrial Cytochrome c Oxidase from Sphyraena barracuda" R. De Jesus, J. López -Garriga and T. Tosteson 29th Junior ACS Tech. Meet. Mayagüez, P.R. March 1994 12. "Monomeric Cytochrome c Oxidase from Shark" C. Ramos-Acevedo, J. LópezGarriga, 29th Junior ACS Tech.Meet. Mayagüez, Puerto Rico. March 1994 13. "Interactions of Mitochondrial Inner Membrane with Ciguatoxins" Reynaldo De Jesus and J. López -Garriga, 10th Forum, School of Medicine, University of Puerto Rico. April 1994 14. "Isolation and Characterization of Halobacterium" N. Rodriguez J. López -Garriga. 6rd Biannual RCMS Meeting, Ponce, P.R. November 1994 15. "Photosensitization of TiO2 by Cu-Phthalocyanine" M. Sandoval, J.J. López -Garriga, 30th Junior ACS Tech.Meet. Ponce ,PR. March 1995 16. Yellow Fractions Identification from Lucina pectinata Hemoglobins S. Ramos, M. Almeida, E. Silfa, J. López -Garriga. 31st ACS Junior Tech. Meet. Arecibo. P.R. March 1996 17. Tropical Sea-atmosphere Measurements of DMS and VHOC. I. Vazquez, D. Acosta, J. López -Garriga. 31st ACS Junior Tech. Meet. Arecibo. P.R. March 1996 18. Photosensitization of Titanium Dioxide by Copper Phthalocyanines M. C. Abad, M. Sandoval, J. López -Garriga. 31st ACS Junior Tech. Meet. Arecibo. P.R. March 1996 19. Vibrational Analysis of Cu (II) Oxamide and Biuret Complexes N.M. Rivera, S. Hernandez, J. López -Garriga. 31st ACS Junior Tech. Meet. Arecibo. P.R. March 1996 20. Spectroscopic and Kinetic Studies of Cytochrome c Oxidase from Marlin. J. Cerda, Z. Sanoguet, , A. Irrizarry, J. López -Garriga. 31st ACS Junior Tech. Meet. P.R. March 1996

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21. Investigation of the Natural Reductant of HbI from Lucina pectinata M. Rodriguez, J. Colon, J. López -Garriga. 31st ACS Junior Tech. Meet. P.R. March 1996 22. Relationship between VHOC and chlorophyll in the Tropics. I. Vazquez, D. Acosta, J. López -Garriga. 32st ACS Junior Tech. Meet. San Juan. P.R. March 1997 23. "Isolation of Hemoglobins II and III from Lucina pectinata" G. Lopez, C. Ramos, Z. Sansoguet J. Lopez-Garriga 32st ACS Junior Tech. Meet. San Juan. P.R. March 1997 24. "Science on Wheels: A Coherent Link Between Educational Perspectives" R. Camacho, M. Camacho, J. López -Garriga 21 th ACS Senior Meet. Lajas, P.R November 1997 25. GLOBE: An Outreach Program that Links International Educational Efforts. R. Camacho, W. Nazario, D. Mora, W. de Jesus, J. Fuentes, M. Camacho, J. López -Garriga 21ACS Senior Meet. Lajas, P.R. Nov. 1997 26. Identification of mRNA of Hemoglobin II from Lucina pectinata. E. Torres F.M. Antonmattei, C. Cadilla, J. López -Garriga, 35th ACS Junior Tech. Meet. P.R. March 200 27. Preparative Isoelectric Focusing: A Technique for Hemoglobin Separation. A. Montalvo, Z. Sanoguet, J. López-Garriga, 35th ACS Junior Tech. Meet. P.R. March 2000 28. FTIR Studies of HbI cyano derivatives from L. pectinata Y. Ortiz, J. López-Garriga. 36th ACS Junior Tech. Meet. P.R. March 2001 29. Preparative Isoelectric Focusing: An appropriate Technique for HB Separation. C. Ramirez, W. De Jesus, J. López -Garriga 36th ACS Junior Tech. Meet. P.R. March 2001 30. Scale-up Production of HbI for Further Spectroscopic Studies A. Roman, V. Collazo, C. Cadilla, J. López -Garriga ,36th ACS Junior Tech. Meet. P.R. March 2001 31. cDNA Sequence of HbII from Lucina pectinata. Elineth Torres, J. López -Garriga SigmaXI Poster Day, UPR-Mayagüez, P.R. April 2001 32. Resonance Raman Studies of methydroxide Hemoglobin I complex of L. pectinata A. Cruz, J. López-Garriga, SigmaXI Poster Day, UPR-Mayagüez, P.R April 2001 33. Ultrafast Geminate Recombination of CN with Hemoglobin I from L. pectinata R. Pietri, C. Ruiz, C. Ramos, J. López-Garriga SigmaXI Day, Mayaguez,PR April 2001 34. Formation of Compound I and Compound II Ferryl Species in the Reactions of Hb I from Lucina pectinata with Hydrogen Peroxide Walleska De Jesús, E. Ramírez, J. López-Garriga, Sigma XI Poster Day, UPR-Mayagüez, P.R April 2001 35. GLOBE-Al Science at Local Forums M. Vélez, R. Camacho, J. López-Garriga, Sigma XI Poster Day, UPR-Mayagüez, P.R. April 2001 36. Cloning and Expression of Unique H2S Binding Hemoglobin from L. Pectinata A. Roman, V. Collazo,C. Cadilla, J. López -Garriga, Sigma XI, UPR-Mayagüez, P.R. April 2001 37. Cloning and Expression of Unique H2S Binding Hemoglobin from L. Pectinata A. Roman, C. Cadilla, J. López -Garriga Biotechnology Day, Mayagüez , Puerto Rico May 2001 38. “Science on Wheels: Science Education Incentives for k-12 Students” Lugo C. Camacho, R., López -Garriga, J. 22nd Puerto Rico Interdisciplinary Scientific Meet. March 2002 39. Ultrafast Studies of Ferrous and Ferric Complexes with Hb I fro m Lucina pectinata. L. López, E. Ramírez, C. Ramos, R. Pietri, M. Rodríguez, M. Chergui, J. López-Garriga. XIV Undergraduate Research Symposium. Metropolitan University, San Juan, P.R. Oct 2003 40. Tyrosine B10 and heme interactions in the HbII from L. pectinata. L. Granell, W. De Jesús Bonilla, A. Cruz Balberdi, E. Torres Mercado, A. Lewis Ballester, R.G. León, C. Cadilla, L. Kiger, J. López Garriga. Metropolitan University (UMET), San Juan, P.R. October 2003 41. Determination of conformations of the HbII from L. pectinata by FTIR. Laura G. Granell, R. Pietri, A. Lewis, C. Cadilla, J. López -Garriga. ACS Junior Tech. Meet. March 2004 42. Expression and Purification of hemoglobin I from Lucina pectinata, using FPLC. A. Quiñones, R. Pietri, J. López -Garriga. ACS Junior Tech. Meet. March 2004 43. FTIR Determination of conformations of the HbII from L. pectinata. L. Granell, R. Pietri, A. Lewis, C. Cadilla, J. López -Garriga SigmaXI Poster Day, UPR-Mayagüez, P.R April 2004 44. Determination of the open and close conformations of the HbII from L. pectinata by FTIR. Laura G. Granell, R. Pietri, A. Lewis, R. Leon, C. Cadilla, J. López -Garriga. Spring Conference in Protein Research, COBRE II-NIH –UPR Center, San Juan. April 2004

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45. Montes-Rodríguez, I., Ariel Lewis, Ruth G. León, Carmen Cadilla, Juan López Garriga, Expression and Purification of HbI mutants in Escherichia Coli. 25th Puerto Rico Interdisciplinary Scientific Meeting & 40th ACS Junior Technical Meeting, Mayagüez, March 2005 46. Aliana López, J. López-Garriga. Crystallization of heme-proteins from Lucina pectinata. X Symposium of Sigma Xi, Chemistry Building, UPR-Mayagüez, P.R. April 2005 47. Aliana López, J. López-Garriga, Crystallization of heme-proteins from Lucina pectinata AABRE External Advisory Committee, Caribe Hilton Hotel, San Juan. May 2005. 48. L. Uchima, A. Cruz, E. Ramirez, A. Santana, E. Lopez, j. Lopez-Garriga, Molecular dynamics simulations of Hemoglobin I active site-mutations. 26th Puerto Rico Interdisciplinary Scientific Meeting & 41th ACS Junior Technical Meeting, UPR-Cayey, March 2006 49. K. Lopez-Alvarz, J. Lopez-Garriga. Purification of recombinant HbI and HbI mutants. 26th Puerto Rico Interdisciplinary Scientific Meeting & 41th ACS Junior Technical Meeting, UPR-Cayey, March 2006 50. Ligand binding kinetics of Hemoglobin II and HbIITyrB10Phe from Lucina pectinata overexpressed in Escherichia coli. Gustavo Afanador. Third Latin American and Caribbean Biotechnology congress. Mayaguez P.R. September 2006 51. Hemoglobin I (HbI) from Lucina pectinata Microinmobilized into Tetramethylorthosilicate Matrix Interacting with Hydrogen Sulfide. Evamariely García, Tatiana Bohec, Shariana Cordero, Edgar Rivera. Advances in Chemical Sciences & Engineering in Puerto Rico XXX ACS. Dorado Del Mar Becah &Golf Resort. November 2006 52. Hemoglobin I from Lucina pectinata Microinmobilized into Tetramethylorthosilicate Matrix Interacting with Hydrogen Sulfide E. Garcia, T. Bohec, S. Cordero, E. Rivera, J. López-Garriga. XXX Senior Technical Meeting “ Advances in Chemical Sciences and Engineering in Puerto Rico. Dorado P.R. November 2006 53. Initial studies of the Kinetic Growth of Symbiotic Bacteria in Lucina pectinata S. Ayala-Conde, M. Ortega-Núñez, J. López-Garriga. XXX Senior Technical Meeting “ Advances in Chemical Sciences and Engineering in Puerto Rico. Dorado P.R. November 2006 54. Valentin, C., Roman, C., Torres-Lugo, M., Lopez-Garriga, J. The assessment of the biological stability and activity of four model proteins encapsulated in ionic hydrogels membranes. AABRE External Advisory Committee, Caribe Hilton Hotel, San Juan. Dec. 2006 55. Garcia, E., Bohec, T., Cordero, S., López-Garriga, J., Hemoglobin I from L. pectinata Microinmobilized into tetramethylorthosillicate matrix interacting with hydrogen sulfide. AABRE External Advisory Committee, Caribe Hilton Hotel, San Juan. Dec. 2006 56. Afanador, G., Bohec, T., Kiger, L., Baudin, V., Marden, M.C., Cadilla, C.L., López Garriga, J. Over expression of HbII and mutants, from L. pectinata, in E. coli. . AABRE External Advisory Committee, Caribe Hilton Hotel, San Juan. Dec. 2006 57. Tatiana Bohec Hemoglobin I from Lucina pectinata Microimmobilized into Tetramethylorthosilicate Matrix Interacting with Hydrogen Sulfide.. 27th Puerto Rico Scientific Meeting & 42nd Junior ACS Technical Meeting. UIA-Bayamón March , 2007 58. T. Bohec, Shariana Cordero Hemoglobin 1 form Lucina pectinata Microinmbilized into Tetramenthylorthosilicate Matrix Interacting with Hydrogen Sulfide.. XXXI Senior ACS Technical Meeting, Mayagüez Resort & Casino, Mayaguez P.R. November 2007 59. Rivera, Christian, Lopez-Garriga, J. Reactivity of hydrogen sulfide with immobilized hemoglobin in Tetramethylortosilicate. 43th ACS Junior Technical Meeting, University of Puerto Rico-Arecibo. March 2008 60. Marchany-Rivera, D., Rodríguez-Medina, E., Román-Morales, E. M., López-Garriga, J. Crystallization of Sulfhemoglobin. 43th ACS Junior Technical Meeting. Arecibo,P. R. March 2008 61. Nieves, Abner, Pietri, Frances, Pietri, R., Lopez-garriga, J. The study between the reaction of Hbl (FeIII) with H2S. . 43th ACS Junior Technical Meeting, University of Puerto Rico, Arecibo Campus, March 2008 62. Pietri, Frances, Nieves, Abner, Pietri, R., Lopez-Garriga, J. Determining the limit of H2S needed in the reaction of HbI (FeIII) H2S. + e-□ HbI (FeII) + H2S (deliver) for the formation of the deoxy product. . 43th ACS Junior Technical Meeting, UPR. Arecibo Campus, March 2008 63. Pietri, Frances, Nieves, Abner, Pietri, R., Lopez-Garriga, J., Study of The Reaction Between Hemoglobin

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I from the Clam Lucina pectinata and Hydrogen Sulfide, Puerto Rico Interdisciplinary Scientific Meeting: Junior Technical Meeting (JTM/PRISM) March 2008 64. Encarnacion, Luis G., Ramos C., Lopez-Garriga, J. Generation of Light Continuous from Transparent Media. 43th ACS Junior Technical Meeting, UPR, Arecibo, March 2008 65. Báez, Pedro, Ortega, M., Lopez-Garriga, J. Development of a Sol-Gel Based Electrochemical Biosensor for the detection of Hydrogen Sulfide. 43th ACS Junior Technical Meeting, University of Puerto Rico, Arecibo Campus, March 2008 66. Burgos, Melisa, Meléndez, Nayda, Lorenzo, Wilmarie, Lopez-garriga, J. Expression and Purification of Recombinant Hemoglobin II from the clam Lucina pectinata. . 43th ACS Junior Technical Meeting, University of Puerto Rico, Arecibo Campus, March 2008 67. Rodríguez, Josiris. Marrero, María del Carmen , Lopez-garriga, J. Oxidation of hemoglobins II and III from L. Pectinata. 43th ACS Junior Technical Meeting, UPR A March 2008 68. Alexandra Martinez-Miller1, Celimar Valentín-Rodríguez, Juan López-Garriga, Madeline Torres-Lugo, Stability of lysozyme crystals in organic solvents and monomer, 43th ACS Junior Technical Meeting, University of Puerto Rico, Arecibo Campus, March 2008 69. Pietri, Frances, Nieves, Abner, Pietri, R., Lopez-Garriga, J., Reaction Between Hemoglobin I from L. pectinata and Hydrogen Sulfide, 32nd Senior Technical Meeting (PR ACS), Rincon P.R. November 2008. 70. Marchany, Darya, Hernández, R.M., Román, E.M., López-Garriga, J., Sulfhemoglobin: Factors affecting its formation. 32nd Senior Technical Meeting of the ACS Rincon, Puerto Rico. November 2008 71. Marrero, María del C., Rodríguez, J., López-Garriga, J.,Oxidation of the hemoglobins II and III from the clam Lucina pectinata. 32nd Senior Technical Meeting of the ACS Rincon, Puerto Rico. November 2008 72. Nieves, A., Pietri, F.M., Pietri, R., López-Garriga, J., Study of the Reaction between HbI from L. pectinata and Hydrogen Sulfide. 32nd Senior Technical Meeting of the ACS Rincon, Puerto Rico. November 2008 73. Hernández-Mercado, R.M., Marchany-Rivera, D., Román-Morales, E.M., Ramos, B.J., Cruz-Díaz, J., Lopez-Garriga, J. Unusual Heme Proteins and their Formation of Sulfhemoglobin. Junior Technological Meeting PRISM. Rio Piedras, PR. March 2009. 74. Formation of Sulfmyoglobin with Sodium Dithionite. Garcia, Erika; Roman, E.; Feliciano, W. Forestier, I., Miranda, B., López-Garriga, J. 45th ACS Junior Technical Meeting, Puerto Rico March 2010. 75. Study of Sulfhemoglobin's Stability; Andujar. B, Marchany, D. López-Garriga, J. 45th ACS Junior Technical Meeting, Puerto Rico, March 2010 76. Myoglobin Encapsulation in Solgel. Berrios, Grace; Rosario, Ivonne; López-Garriga, J. 45th ACS Junior Technical Meeting, Puerto Rico March 2010 77. Egg-white Lysozyme Crystallization by Equilibrium Techniques. Marrero, Ulises. Rodriguez Josiris. Lopez-Garriga, J. 45th ACS Junior Technical Meeting, Puerto Rico March 2010 78. Exploring the Structure and Function of recombinant HbI from L. Pectinata; Ruiz, Doribeth, Ramos, Brenda, López-Garriga, J. 45th ACS Junior Technical Meeting, Puerto Rico March 2010 79. Role of Histidine in Sulfheme Complex. Ortiz, N. Lopez-Garriga, J. 45th ACS Junior Technical Meeting, Puerto Rico March 2010 80. Chemical Reaction Between Mercaptans with Mb:Acidity vs. Steric Hindrance Complex Formation, Quinones, T., López-Garriga, J, 45th ACS Junior Technical Meeting, Puerto Rico March 2010 81. Catalase from Bovine Liver in the Sulf Scenario; Pietri, Frances, López-Garriga, J. 45th ACS Junior Technical Meeting, Puerto Rico, March 2010 82. Purification of hemoglobins from L. Pectinata. Munoz, C., Feliciano, W., Diaz, K., Delgado, C.,Rodríguez, C., González, J., Lopez-Garriga, J. 45th ACS Junior Technical Meeting, Puerto Rico, March 2010 83. Hydrogen Sulfide and Heme-Oxygen Complez Activation; Marrero, M., Román-Morales, E., López-Garriga, J. 45th ACS Junior Technical Meeting, Puerto Rico. March 2010 84. E. Y. García-Gutiérrez, E. López-Alfonso, E. Román-Morales, J. López-Garriga, Sulfmyoglobin fromation with nitric oxide. 34th Senior Technical Meeting, Mayagüez P.R. November 2010 85. Forestier,I., Román Morales, E., López Garriga, J. Sulfmyoglobin fromation with sodium ditthionite. 34th Senior Technical Meeting, Mayagüez P.R. November 2010 86. Quiñones-Ruiz, T., López-Garriga, J. Kinetics of insulin Amyloid fibrils formation in polymeric membranes. 34th Senior Technical Meeting, Mayagüez P.R. November 2010 87. Marrero-Llerena, U., Rodriguez, J., López-Garriga, J. GG-white lysozyme crystallization. 34th Senior

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Technical Meeting, Mayagüez P.R. November 2010

High School Students Presentations in Local Technical Meetings (13) 1. Factors Influencing the C=N Bond in Schiff's Bases" A.M. Minguela, J.J. López-Garriga, VIII Meet. of High School Summer Research, Ponce, P.R. July 1990 2. Binding of CO in Cytochrome Oxidase L.A. Acevedo-Torres and J.J. López-Garriga, VIII Meeting of High School Research, Catholic Univ.of Puerto Rico, Ponce, P.R. July 1990 3. Cytochrome Oxidase Extracted from Beef Heart" R. Valentin, J.J. López-Garriga, IX Meeting of High School Summer Research, Univ. of Puerto Rico, Rio Piedras, P.R. July 1991 4. UV-VIS Studies of Reduced Mitochondrial Cytochrome Oxidase C. Ramos-Acevedo and J.J. López-Garriga, IX Meeting of High School Summer ,Rio Piedras, P.R. July 1991 5. Schiff's Bas Quiñones-Ruiz, T., López-Garriga, J. Kinetics of insulin Amyloid fibrils formation in polymeric

membranes. 34th Senior Technical Meeting, Mayagüez P.R. November 2010e Models of the Retinal C=N Bond Bacteriorhodopsin. W. Bonaparte and J.J. López-Garriga, IX Meet. of High School Summer Research, Univ. Puerto Rico. July 1991 6. Synthesis of Protonated Schiff's Bases. W. Bonaparte, J. Lastra and J.J. López-Garriga, X Meet. of High School Summer Research, University of Puerto Rico July 1992 7. Presence of Cytochrome Oxidase in Sea Cucumber Holothuria glaberrima. Carmen B. Veiga, J.J. López-Garriga, X Meeting of High School Summer Research, U.P.R. July 1993 8. Kinetics of H2S binding to HbI from Lucina pectinata. Alexandra Marie Saavedra. Puerto Rico Science fair, grade 10. Mayagüez P.R. (1er position Regional) December 2002 9. Relationship between dissolved O2 and Salinity in H2O. M. Lefebre. Puerto Rico Science Fair, grade 10. Mayagüez P.R. (1er position Regional) December 2002 10. Relationship between Kinetics of H2S binding to HbI from L. pectinata . A. Marie Saavedra. P.R. Science Fair, grade 11. (1er position Regional) Mayagüez, P.R. December 2003 11. Relationship between Kinetics of H2S binding to HbI from Lucina pectinata and Chemical Structure. A. Marie Saavedra. AMP-Puerto Rico, February 2004 12. Relationship between Kinetics of H2S binding to HbI from Lucina pectinata and Chemical Structure. A. Marie Saavedra. Intel International Science and Engineering Fair. Portland, Oregon, May 2004 13. Kinetics of H2S binding to HbI from Lucina pectinata and Chemical Structure. A. Marie Saavedra. Junior Science and Humanities Symposium, Maryland. May 2004. 14. Sulfhemoglobin formation of GlnE7His mutant of HbI from L. pectinata. L. Ruiz-Nieves, Bessie Rios, J. Lopez-Garriga STEP-UP program NIH, Washington D.C. September 2010 15. Sulfhemoglobin formation from NO y H2S and myoglobin. Erika Garcia, Elddie Roman, J. Lopez-Garriga STEP-UP program NIH, Washington D.C. September 2010 16. Reaction of H2S with hemoglobins from Mercenaria mercenaria. M. Galloza, I. Montes, J. Lopez-Garriga STEP-UP program NIH, Washington D.C. September 2010 17. Isolation of hemoglobins from Codakia Orbicularis. L. Rosa-Lugo, I. Montes, J. Lopez-Garriga STEP-UP program NIH, Washington D.C. September 2010 18. Flash photolysis of hemoglobins CO. J. Roman Rodriguez, H. Arbelo, M. Rosario, J. Lopez-Garriga STEP-UP program NIH, Washington D.C. September 2010 19. Crystallization of the HbI-Cystein protein complexes. A. Moya-Rodriguez, J. Rodriguez, R. Estremera, J. Lopez-Garriga STEP-UP program NIH, Washington D.C. September 2010 20. Characterization of Carbon Nanotubes modified with rHbI and electrochemical techniques. K. Muniz-Rodriguez, R. Diaz-Ayala, J. Lopez-Garriga STEP-UP program NIH, Washington D.C. September 2010