1

Curriculum Vitae

Name Dr. MUHAMMAD ALI

Nationality Pakistani

Date of Birth March 7, 1983

Marital Status Married

Expertise of spouse She is pharmacist in General Hospital, Faisalabad, Pakistan.

Moreover, she is Ph.D. scholar of Pharmacology from UAF,

Pakistan.

Current Position Assistant Professor (Biochemistry, Molecular & Cell Biology)

Incharge Cell Culture and Research Lab (CCRL), FIRST

Incharge Laboratory Animal Research Center (LARC), FIRST

Incharge Mammalian Molecular Genetics Lab (MMGL), FIRST Akhuwat Faisalabad Institute of Research Science and Technology (FIRST),

HEC Approved Supervisor HEC Approved Supervisor of Biological and Medical Sciences

Fields of Specialization Biochemistry, Cell and Molecular Biology, Metabolism

[Gene Expression, Cell Signalling, Cancer Biology, Oncogenetics,

Diabetes, Liver injury, CRISPR/Cas9, siRNA, Molecular Cloning,

Gene Editing in Mice, Molecular Genetics, IHC, ICC, Flow

cytometry]

Postal Address P-539/A, Street # 5, Shadab Colony, Jhang Road, Faisalabad, 38000

PAKISTAN

Official Postal Address Akhuwat FIRST City Office (Dr. Ehsan-ul-Haq Clinic),

P-69, Jinnah Colony, NearChatri Wali Ground, FAISALABAD

Email Addresses mali@gcuf.edu.pk; muhammadali.1@hotmail.com

(Cell.) +92-333-6606630, Skype: muhammad.ali1114

Qualification

2012-2017 Ph.D. (Biochemistry) CGPA: 3.85/4.30 (First Class)

Yonsei University, Seoul, South Korea

Specialization: Molecular & Cell Biology

Thesis Title: TERT Induces Autophagy by Regulating the Kinase Activity of mTORC1

2010-2012 M.Phil. CGPA: 3.94/4.30 (First Class)

Yonsei University, Seoul, South Korea

Major courses studied: Advanced Biochemistry, Analytical Chemistry

Thesis Title: Flow Field-Flow Fractionation / Laser Light Scattering Analysis of

Polysaccharides for Medical Applications

2005-2007 M.Sc. CGPA: 3.78/4.00 (First Class)

University of Agriculture, Faisalabad, Pakistan (UAF)

Major courses studied: Analytical Chemistry, Biochemistry

Thesis Title: Antioxidant and Antimicrobial Potential of Essential oil and Various

Extracts of Fennel (Foeniculum vulgare Mill.) seeds

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Job experience

Post Held BPS / Salary

Institution / Organization

Duration

From To Year Month Assistant Professor BPS-19 Akhuwat FIRST April 2019 Continue 0 9

Assistant Professor Visiting Akhuwat FIRST 08 March 2018

March 2019 1 0

Assistant Professor IPFP GCUF March 2017 March 2018 1 0

Research Associate Equal to BPS 17

Yonsei University, South Korea

March 2010 Dec 2011 1 9

Research Associate Equal to BPS 19

Yonsei University, South Korea

March 2015 February 2017 2 0

Scientific Assistant SPS-III NIBGE, Faisalabad May 2003 Feb 2010 6 10

Total Experience 13 4

Publications [IF is 24.569; Citations 152] .

1. Ali, M.*, et al., Upstream Signalling Regulates Hyperactivation of mTORC1 in Type 2 Diabetes and Hypertriglyceridemia. BMB reports 2017. 50(12): 601‐609 [IF = 3.089]

2. Ali, M., et al., Telomerase reverse transcriptase induces basal and amino acid starvation-induced autophagy

through mTORC1. BBRC, 2016. 478 (3): p. 1198-1204. [IF = 2.466]

3. Devkota, S., Jeong, H., Kim, Y., Ali, M., et al., Functional characterization of EI24-induced autophagy in the degradation of RING-domain E3 ligases. Autophagy, 2016. 12 (11) 2038-2053. [IF = 11.753]

4. Ali, M., et al., Extracting Extra-Telomeric Phenotypes from Telomerase Mouse Models. Yonsei medical journal,

2014. 55(1): p. 1-8. [IF = 1.537]

5. Mustafa, G., Arif, R., Bukhari, SA, Ali, M., Sharif, S. and Atta, A. Structural and functional annotation of

citrate synthase from Aspergillus niger ANJ-120. PJPS 2018. [IF = 0.649]

6. Ali, M., et al., Characterization of sodium hyaluronate blends using frit inlet asymmetrical flow field-flow

fractionation and multiangle light scattering. Analytical and bioanalytical chemistry, 2012. 402(3): p. 1269-

1276. [IF = 3.431]

7. Anwar, F., Ali, M. et al., Antioxidant and antimicrobial activities of essential oil and extracts of fennel seeds from Pakistan. Flavour and fragrance journal, 2009. 24(4): p. 170-176. [IF = 1.644]

Books/Manuals . 1. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2018, 1st edition, ISBN: 978-969-23317-0-8.

2. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series II), 2019. 1st edition, ISBN: 978-969-23317-2-2.

3. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2019, 2nd edition, ISBN: 978-969-23317-1-5.

4. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series III), xxxx, 1st edition, ISBN: 978-969-23317-x-

x. (In write up)

Development of advanced lines (verities) of rice .

1. I have played an important role (2003-2010) in the development of bacterial blight resistant advanced lines

of Super Basmati (rice variety) (Our rice advanced line BR1 got the top position and showed the highest

yield in National Uniform Rice Yield Trial 2013) [National Institute for Biotechnology and Genetic

Engineering, Faisalabad]

Patents submitted to .

1. Bukhari, S.A., Ali, M., Salman, M., Anjum, F. Identification of new strain of Lactobacillus coryniformis from rice

rinsed water (receipt: 3176301, dated: 03/01/2018; patent application # 666/2017, dated: 19/12/2017)

2. Bukhari, S.A., Salman, M., Ali, M., Hussain A.I. Identification of new strain of Lactobacillus plantarum from rice

rinsed water (receipt: 3176302, dated: 03/01/2018; patent application # 667/2017, dated: 19/12/2017)

3. Bukhari, S.A., Anjum, F., Ali, M., Shahid M. Nanoparticles encapsulation of date palm mucilage for colonic drug

delivery (receipt: 3176468, dated: 24/01/2018; patent application # 49/2018, dated: 24/01/2018)

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Research summary of Dr. Muhammad Ali .

My expertise lies in solving cell-signalling pathways using cancer cell lines, genetically engineered (GMO) &

chemically induced mouse models of human diseases like diabetes, liver injury, and breast cancer. I majorly work

on the functional role of PI3K-AKT-mTORC1 and MAPK-ERK cell signalling pathways. I study the pheonomena

and signalling of autophagy and mitophagy in diabetes, breast & liver cancers. I usually suggest to adopt the idea

of signalling modulation rather than merely inhibition. To understand these signaling pathways, I use tools of genetic

engineering (CRISPR/Cas9), RNA interference (shRNA, siRNA), protein interaction (Co-IP, Western blotting,

ICC), and tissue phenotype (IHC).

My first discovery came out when I found a novel role of TERT in the inhibition of kinase activity of mTORC1.

Furthermore, TERT-mediated inhibition of mTORC1 induced basal and amino acid starvation autophagy and thus

cell survival. Second, I discovered that EI24 protein degrades TRAF6 to attenuate MAPK/ERK and STAT3

signaling pathways that implicate breast cancer. I also remained extensively involved in the generation of genetically

modified mice for human diseases models. I have written some projects to suggest therapeutic strategies for

diabetes and liver fibrosis. These days, I am also included several medicinal plant to treat diabetes and liver injury.

WAT

LPL

TriglycerideOver-nutrition

Amino acid

Vagal nerve Sympathetic nerve

LPL

mTOR

S6K

TG secretion

Communication from liver to brain to adipose tissues

Brain

TG

Glucneo.

SREBP1

IR

Fatty liver

IR

Blood TG

Autophagy links hyperglycemia and fatty liver (Cross talk among liver, adipose tissue, and brain)

In-vivo

CRISPR-Cas9 are delivered, on a vehicle, to

the patient for gene therapy

CRISPR-Cas9 are used for genetic engineering of

cultured cells/cells

Therapeutically modified cells are

expanded and returned to the patient

Ex-vivo

Cells are removed from

patient

Gene therapy (Future plan)(Gene therapy by using tools of genetic engineering)

TSC1

TSC2

P

PPP

RHEBGTP

RHEBGDP

mTORC1

PI3KAKT

P P

PIP3PIP2

CYTOSOL

Grb2 RasGTPSOS

RTK

TERT

Isolation membrane

ULK1/2Atg13 FIP200

PAtg14L Becn1

Vps34

S6KP

4E-BP1P

LC3-II

EI24

TRAF6

ATM

Radiation

Raf

MEK1/2

Erk1/2

NUCLEUS

Lipid metabolism

Survival Translation

Transcription Acetylation

Erk1/2

P

P

PI3K-AKT-mTOR and MAPK/ERK signaling pathways

GFP-LC3-II

Autophagolysosome

Biochemistry, Molecular and Cell biology FUNCTION

GENE PROTEIN

Recombinant DNA technology

(CRISPR/Cas9)

Citrate

Isocitrate

α-ketoglutarate

Succinyl CoA

Succinate

Fumarate

Malate

Oxaloacetate

Aconitase

Isocitrate

dehydrogenawe

α-ketoglutarate

dehydrogenase

Succinyl CoA

sunthetaseSuccinyl CoA

dehydrogenase

Fumarase

Malate

dehdrogenase

Citrate

synthase NAD+

NADH + H+

NAD+ + CoA

NADH + H+

+CO2

GDP + Pi

GTP +CoAFAD

FADH2

H2O

NAD+

NADH + H+

H2O +

Acetyl CoA

CoA

Citric acid cycle

Indels

Double strand break

Premature stop codon

PAM sequence

Cas9 cuts both strands, 3 bp away from PAM sequence

XX

PAM sequence

gRNA

NUCLEUS

CRISPR/Cas9 Technology for Genome Editing

Molecular biology

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Professional training taken . 1. Pakistan 2018 2018 1st National workshop on Basics of Bioinformatics.

2. Korea 2012 2015 Radioactive safety and operational training for life science

3. Korea 2012 2016 Experimental animal management and handling training

4. Korea 2010 2016 Laboratory and chemical safety training

5. Korea Jan-2012 2012 Course for mouse necropsy for in vivo experiment

6. Korea 2013 2016 Laboratory animal science and ethics for life science

7. Korea 2012 2012 Generation and handling of GEMs for human disease research

8. Pakistan 2009 (12-13 Oct) Hybrid Rice Seed Production Technology

Membership of learned societies .

1. Korea The Korean Society for Biochemistry and Molecular Biology (KSMCB)

2. Pakistan The Pakistan Society for Biochemistry and Molecular Biology (PSBMB)

3. Korea The Korean Association for Laboratory Animal Science (KALAS)

4. Korea The Korean Chemical Society (KCS)

Honors, distinctions & awards .

1. Ph.D. 90% Overseas Scholarship, P-II, B-II, HEC, Pakistan (3.85/4.3) 2012

2. M.Phil. 90% Overseas Scholarship, P-II, B-II, HEC, Pakistan (3.94/4.3) 2010

3. M.Sc. Merit Scholarship, UAF, Faisalabad (3.78/4.00, 78.83%) 2006

4. F.Sc. Distinction-award, GC Science, Faisalabad (76.77%) 2000

5. Matric Stood first in Govt. Farooq High School, Faisalabad (82.00 %) 1999

Submitted project . 1. Changes of mitophagy in the liver of mouse model of diabetes (PI, Rs. 0.452 million SRGP # 21-1477), 2017

2. Therapeutic potential of TERT-mediated restriction of mTORC1 for the treatment of liver fibrosis and chronic hepatitis (submitted as PI, PSF, August 2019. 4.6 million)

3. Therapeutic evaluation of CRISPR/Cas9-mediated knockout and repair of EI24 gene in the breast cancer (submitted as PI, PSF. August 2019. PKR 4.8 million)

4. Evaluation of Therapeutic potential of liver-specific expression of TERT for the treatment of type 2 diabetes (submitted as PI, PSF. August 2019. PKR 4.6 million)

5. Development of primary cell culture adapted attenuated infectious bursal disease candidate vaccine in broiler (submitted as Co-PI, PSF. August 2019. PKR 4.14 million)

6. Study of biological significance of rabbit GDF11 against human small cell lung cancer cells (SCLC) progression (submitted as Co-PI, PSF. August 2019)

7. Evaluating the roles of natural bioactive compounds-mediated induction of mitophagy, autophagy, and mTORC1 activity in the diabetic mouse model of high energy fed diet (submitted as PI, IFS. November 2019. USD 14,900)

8. Evaluation of Selected Medicinal Plants and Bioactive Compounds Against Dengue Virus Serotype Prevalent in Pakistan (will submit in NRPU in December 2019)

9. Molecular determinates and investigation of new targets against mouse model of type 2 diabetes. (will submit in NRPU in December 2019)

Running projects of students . 1. Natural bioactive compounds and crude extracts-induced mitochondrial turnover in the mouse model of type 2

diabetes 2. Preventive effect of hydroalcohlic extracts of Rhazya stricta and Arbutus unedo L. against mouse model of type-

II-diabetes 3. Evaluation of in vitro and in vivo antioxidant potential of Nigella sativa L. and Allium sativum L. extract on the

mouse model of type 2 diabetes 4. Antidiabetic potential of hydroalcoholic extracts of Stevia rebaudiana and Andrographis folium in diet induced

diabetes mouse model 5. Combined anti cancer, antioxidant protective effect of “Artemisia Absinthium” and “Citrus Aurantium” extract

on Albino mice model of hepatitis.

Departmental duties performed .

1. Member of IT committee (FIRST)

2. Member of purchase committee (FIRST)

3. Management of departmental events / functions (FIRST)

4. Member Equipment purchase committee (FIRST)

Students produced as Assistant Professor .

1. Supervised 2 M.Phil. students (GCUF)

2. Supervised 15 BS (H) students (GCUF)

3. Supervising 8 BS students for 1 year research plan (Akhuwat FIRST)

4. Supervising 1 M.Phil. student (GCUF)

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Areas of research interests & experience .

Biochemistry, molecular and cell biology:

1. Viability of cells under the regulation of mTORC1 (PI3K-AKT-mTORC1 signaling axis)

2. Cancer cell survival in relation to autophagy (TERT-mTORC1-S6K signaling axis)

3. Molecular signalling of breast cancer cells (RAS-RAF-MEK-ERK sinaling axis)

4. Role of Autophagy and mitophagy in diabetes and its signalling pathways

5. Functional studies of PI3K-AKT-mTOR signaling pathway and its implication to liver cancer

6. The role of TERT in the inhibition of mTORC1 that relieves liver cancer cells from ER stress

7. The role of EI24 in breast cancer through the inhibition of MAPK/ERK pathway

8. The role of TRAF6 in the activation of MAPK/ERK signaling in breast cancer

9. CRISPR/Cas9-facilitated genome editing in cancer cell lines and mice

10. Generation of conventional & conditional transgenic and knockout mice for human disease models

11. Testing of natural bioactive compounds against type II diabetes

12. Testing of natural bioactive compounds against liver injury

13. Testing of natural bioactive compounds against dengue disease

14. Solving signalling pathway for fatty liver disease

15. Pathway finding by in-silico analysis (correlation of gene signature)

Bio-analytical chemistry:

16. Extraction and activity guided the evaluation of natural antioxidant extracts from indigenous plant

sources (separation, identification, and quantification)

17. Extraction of essential oils of herbal and medicinal importance by various classical and non-classical

methods and their characterization

18. Antioxidant and antimicrobial activities of extracts from the indigenous plant

19. Analytical approach to characterize polysaccharides of the range of 1E6 g/mole to 100E6 g/mole for

surgical applications using field flow fractionation and laser light scattering approach

20. Flow-Field flow fractionation/laser light scattering analysis for the blends of polysaccharides for

medical applications

21. In-vitro studies for the interaction of sodium hyaluronate with most abundant proteins (human serum

albumin and ovalbumin)

22. Antioxidant, autophagy, and cell death analysis of cancer cells when provided with unsaturated fatty

acids and steroids

Biotechnology:

23. In-silico study to find pathways leading to oncogenesis by using online cancer database

24. Study of HCC and breast cancer using mouse models and human samples

25. Pyramiding of disease related genes in mouse lines

26. Adulteration testing of Super Basmati, using molecular markers for specific DNA loci, for the export

of Basmati Rice to European countries

27. Molecular marker-facilitated pyramiding of bacterial blight resistance genes in Super Basmati rice

(Our rice advanced line BR1 got the top position and showed the highest yield in National Uniform Rice

Yield Trial 2013)

28. Identification and mapping of bacterial blight resistance genes form Pakistani rice verities

29. Genetic diversity assessment of Pakistani Basmati landraces and Basmati breeding lines using DNA-

based SSR and RAPD analysis

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Expertise of scientific techniques .

Molecular biology techniques:

1. Polymerase chain reaction (primers designing for genotyping, cloning, mutation etc.)

2. Site directed mutagenesis, insertion, deletion (molecular cloning)

3. Gene isolation and gene expression studies

4. Molecular cloning to express specific proteins in cancer cell lines and mice

5. DNA extraction and Southern blotting to check mutations in GEM

6. RNA extraction and Northern blotting to observe transcript size and expression

7. cDNA synthesis and quantitative PCR for gene expression studies

8. Protein extraction, SDS-PAGE and Western blotting for cell signaling studies

9. Competent cells making, transformation, preservation, colony PCR, and plasmid extraction

10. Interaction and localization of proteins by Co-IP and Western blotting

11. Protein extraction for functional study of their domain and their interactions

12. Functional modulation of enzymes by mutations in specific amino acids and domains

13. cDNA synthesis and its cloning in expression plasmids

14. Design and making gene knockdown and knockout system using siRNA/shRNA and CRISPR/Cas9

respectively

15. Invitro synthesis of guided RNA to use in CRISPR/Cas9 system

16. Pyramiding of genes in the mouse for cell signaling study in-vivo

17. PCR (RAPD) based genetic diversity study among members of same species

18. Proximate analysis of plant samples for nutrient values

19. Antioxidant and antimicrobial activities of crude extracts for food chemistry purpose

Cell biology and cellular phenotype studies (In-vitro):

20. The cell culture of all kinds of cell lines including primary, cancer, and embryonic stem cells

21. CRISPR/Cas9-mediated genetic changes in cell lines for the functional studies of enzymes and proteins

22. A lentiviral system to knockdown/knockout genes from cell lines and mice for the functional study of

proteins in-vivo

23. Stable cell line generation by lentivirus-mediated gene knockdown

24. FACS analysis for apoptosis, autophagy induction, and stable cell line making

25. Immunocytochemistry to detect protein localization and interaction

26. MTT assay to check the viability of cells

27. Colony formation assays to check the oncogenic potential of proteins in cancer cell lines.

28. Oil-Red-O staining to check lipid accumulation that leads to inflammation in the liver

In-vivo studies using mouse models:

29. Mouse necropsy and organ collection for in-vivo studies

30. RNA, DNA, and protein extraction and then expression test in different organs of mice

31. Immunohistochemistry analysis to check tissue morphology

32. DEN-mediated carcinogenesis for the study of liver cancer

33. Generation of tissue and organ-specific KO and transgenic mice

Bioinformatics techniques:

34. NCBI primer blast link for designing primers for genotyping and cloning

35. DNA and protein homology using NCBI link, Muscle link, Cluster W link, T-coffee link

36. Oncomine for the expression of genes in cancer tissues link

37. Snap-gene for cloning, genotyping, alignment, and map construction link

38. Molecular Signatures Database for GSEA link

39. Pathway finding by In-silico analysis (cBioPortal and Oncomine) link

40. Analysis of diversity among different members of a species using dendogram (Omega 6)

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Research projects completed/contributed .

Sr.# Title of research Period Institution

1)

Pyramiding of Bacterial blight resistance genes in Super

basmati.(Our rice variety BR1 got the top position and showed

the highest yield in National Uniform Rice Yield Trial 2013)

May-2003 to Feb-2010 NIBGE,

Faisalabad

2) Genetic diversity analysis of Chickpea using RAPD primers 2005 “

3) DNA testing for the purity of Super Basmati for the export to

European countries ~2004-2006 “

4) Antioxidant and antimicrobial activities of essential oil and

extracts of fennel (Foeniculum vulgare Mill.) seeds from Pakistan 2007-2008 (M.Sc.)

University of

Agriculture,

Faisalabad

5)

Characterization of sodium hyaluronate blends using frit inlet

asymmetrical flow field-flow fractionation and multiangle light

scattering (for medical applications)

2010-2012 (M.Phil.)

Yonsei

University,

South Korea

6) Telomerase reverse transcriptase induces basal and amino acid

starvation-induced autophagy through mTORC1. 2013-2016 (Ph.D.) “

7) Functional characterization of EI24-induced autophagy in the

degradation of RING-domain E3 ligases. 2012-2016 (Ph.D.) “

8) Molecular cloning of TRAF6, EI24, TERT, mTOR 2012-2016 (Ph.D.) “

9) The role of TERT in mitophagy 2016-2016 (Ph.D.)

10) EI24 attenuates TRAF6-mediated MAPK/ERK signaling 2015-2016 (Ph.D.) “

11) Pyramiding of 4 alleles, maintenance,

progress report

SM-ApoE-RageA

SM-ApoE-RageC

SM-ApoE-RageD

SM-ApoE-RageD

SM-ApoE-Tlr4C

SM-ApoE-Tlr4D

SM-ApoE-Tlr4E

Jan-2014 to 27-Aug-2015 (20 m)

Jan-2014 to 27-Aug-2015 (20 m)

Jan-2014 to 27-Aug-2015 (20 m)

Jan-2014 to 27-Aug-2015 (20 m)

Jan 2014 to 06-04-2015 (15 m)

Jan 2014 to 06-04-2015 (15 m)

Jan 2014 to July 2015 (18 m)

SIRIC project;

Yonsei

University

12) Maintenance, correspondence, the data

record

GnRH-IRES-t-

GFP-Tg mice

Feb-2013 to Nov-2013 (9

months) Kyung Jin Kim Yonsei University

13) Maintenance, correspondence, data

record, dispatching of mice YAP-Tg mice

Nov-23-2012 to Dec-9-2012

(~1 month) Dae-Sik Lim; Yonsei University

14) Designing and optimization of WT and

KO specific PCR primers CD47 KO mice

Oct 23, 2015, to Dec-3, 2015

(~1 month) CMHD; Yonsei

University

15) Designing and optimization of WT and

KO specific primers CiiTa KO mice

Nov ll,2015 to Dec-7, 2015

(~1 month) “

16)

Screening, maintenance, genotyping,

sperm freezing, record keeping (TALEN-

mediated)

miR99a KO mice 27-03-2015 to 01-10-2015 (6

months) “

17)

Screening, maintenance, genotyping,

sperm freezing, record keeping (TALEN-

mediated)

Tars KO mice 27-03-2015 to 21-01-2016 (19

months) “

18) Maintenance, genotyping, DEN injection,

sent mice to KRIBB Ei24B6 mice

17-09-2014 to 19-11-2014

(1+14 months)

Je Kyung

Seong; Yonsei

University

19) Expression test of p27 KO mice p27 KO 06-10-2016 to 25-12-2016 (~2

month) CMHD; Yonsei

University

20) Changes of mitophagy in diabetic mice Liver/Diabetes August 2017-continue SRGP

21) Screening of Sfrp4 Inhibitors for the

treatment of type 2 diabetes Sfrp4 March-2017-2019 --

22) Studies on molecular determinants of

stem rust in wheat Sr45, Sr22 March-2017-2019 NRPU

23)

Evaluation of Therapeutic potential of

liver-specific expression of TERT for the

treatment of type 2 diabetes

TERT, mTORC1,

diabetes

Submitted as PI, 2019, PSF,

PKR 4.98 million PSF

24)

Therapeutic evaluation of CRISPR/Cas9-

mediated knockout and repair of EI24

gene in breast cancer

EI24-TRAF6-

MAPK-ERK

signaling axis

Submitted as PI, 2019, PSF,

PKR 6.72 million PSF

25)

Therapeutic potential of TERT-mediated

restriction of mTORC1 for the treatment

of liver fibrosis and chronic hepatitis

Liver fibrosis,

CCl4, TERT

Submitted as PI, 2019, PSF,

PKR 4.98 million PSF

26) Three projects submitted as Co-PI with GCUF and FIRST (PSF 2019)

8

Teaching and research experience of (coursework and research work)

1. Assistant Professor of Biochemistry, GC University, Faisalabad March 2017- continue

2. Yonsei University, South Korea (Research project of internee Khanh Chu) 2016

3. Yonsei University, South Korea (Research project of internee Ahn) 2015

4. Yonsei University, South Korea (Partial help in Molecular Physiology) 2014-2016

5. Yonsei University, South Korea (Partial help of Biochemistry 1) 2013-2015

6. Yonsei University, South Korea (Partial help of Biochemistry 2) 2013-2015

7. Yonsei University (Partial assistance in Experimental Biochem, fall semester) 2014

8. Yonsei University (Partial assistance in Experimental Biochem, spring semester) 2013

9. Yonsei University, South Korea (Practical course for Tg and KO mouse technology) 2012, 2013, 2014

10. Scientific Assistant at National Institute for Biotechnology & Genetic Engineering 2003-2010

Suggested courses for teaching

1. Molecular Biology (Lehninger 6E, Stryer 7E) Undergraduate & Graduate

2. Cell Biology (Lodish 6E) Undergraduate & Graduate

3. Metabolism (Lehninger 6E) Undergraduate & Graduate

4. Biochemistry 1 and 2 (Lehninger 6E, Stryer 7E) Undergraduate & Graduate

5. Biochemistry Laboratory (Modern Exp. Biochem., Boyer 3E) Undergraduate & Graduate

6. Genetic Engineering (Brown, internet resources) Graduate

7. Advanced Biochemistry (Lehninger 6E) Graduate

8. Advanced Biochemical Techniques (Biochem. & Mol. Biol., Wilson 6E) Graduate

9. Drug Biochemistry (Rang and Dale 7E) Graduate

10. Nutritional Biochemistry (Gropper 6E) Undergrad

Sports (intervarsity/provincial)

1. Seleted from all Faisalabad Karate selection and played all Punjab karate Tournament. 2008

2. Awarded as top 2 athletes in the annual sports event of Department of Chemistry, Yonsei University,

South Korea, September 2010 (chem@yonsei.ac.kr)

3. Took part in soccer, tug of war etc. in the sports event held among all the students of Yonsei University,

South Korea, September 18, 2010 (global@yonsei.ac.kr)

4. Black belt (1st Dan) holder from grand master Young Sun Kim (Kukkiwon), Yonsei University, South

Korea, February 23, 2014 (kystae@naver.com)

5. Silver medalist of phoomse in International Taekwondo Exhibition and Competition event, Kukkiwon,

Seoul, South Korea, September 27, 2014 (kystae@naver.com)

6. Silver medalist in Khyurgi (fight) International Taekwondo Exhibition and Competition event, Kukkiwon,

Seoul, South Korea, September 27, 2014 (kystae@naver.com)

7. Black belt (2nd Dan) holder from grand master Young Sun Kim (Kukkiwon), Yonsei University, South

Korea, December 6, 2015 (kystae@naver.com)

Extra-curricular and volunteer experience

1. Physical fitness training was obtained from Haji Azam Ali and Majid Butt, Faisalabad, Pakistan (2008-

2009).

2. Physical fitness and Taekwondo training assistant of Grandmaster Young Sun Kim at Yonsei International

Taekwondo club, Yonsei University, South Korea, (2014-2016).

3. A representative of HEC student to represent matters in the graduate school office of Yonsei University

(2010-2013).

4. Participation in indoor activities in the yearly gathering of Korean and international students at Yonsei

University, Seoul, South Korea (2010-2015).

5. Participation in the social and national events organized by Pakistani student in South Korea (2010-2016).

6. Worked in the executive committee in the foundation and continuation of Sinchon Masjid in South Korea

(2015-2017).

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Participation in international symposia/ conferences

1. Organized one-day symposium entitles “intellectual Disabilities and Brain Health”. GCUF, 13 December 2019

2. One-day workshop on the tools of bioinformatics. UMT, Lahore. 07 December 2019.

3. Organized One day seminar on ‘Recent Trends in Biotechnology’ and Science Exhibition. Akhuwat FIRST. 29

November 2019.

4. Conducted hands on training on “Primer Designing and Troubleshooting” GCUF, 17 October 2019

5. Organized and delivered lecture in 1st International Symposium on “CRISPR-based genome editing and

precision medicine” 18 December 2018.

6. Project Execution: PPRA rules”. GC University, Faisalabad, Pakistan, 22-26 January 2018

7. Secretary of the event ‘An Awareness Seminar on Diabetes’, GC University, Faisalabad, Pakistan, 14

November 2017

8. KSBMB annual meeting. Young-Pyong, Gangwon Province, Korea, January 2016

9. KSBMB International Conference 2015, COEX, Seoul, Korea, May 12-14, 2015

10. Northeastern Asian Symposium on Autophagy, Busan, Korea, December 19-20, 2014

11. Autophagy symposium. Yang-Yang city, Korea, August 28-29, 2014

12. 2nd international symposium of RCCS (Research Centre for Cellular Homeostasis), Ehwa Women University,

Seoul, South Korea, June 20, 2014

13. 2014 KSBMB annual meeting, COEX, Seoul, Korea, May 14-16, 2014

14. Global Academic Programs 2014 Conference, Grand Hilton, Seoul, Korea, May 1-3, 2014

15. The Korean Association for Laboratory animal science, winter symposium, South Korea. February 20-21, 2014

16. Mouse genetics symposium, Yonsei University, South Korea, December 18, 2013

17. 2013 KSBMB annual meeting. Young-Pyong, Gangwon Province, Korea January 2013

18. Dasan conference for mouse phylogenomics, Seongwipo KAL Hotel, Jeju Island, September 23-25, 2012

19. The 23rd KSMCB winter conference. Yong Pyong Resort, Gangwon Province, South Korea, February 1-3,

2012

20. 2011 annual meeting of KSMS and 2nd AOMSC, Busan Exhibition & Convention Center (BEXCO), South

Korea, August 17–19, 2011

21. The 107th National Meeting (spring) of the Korean Chemical Society, ICC, Jeju, Korea, April 28-29, 2011

22. Fifth International Symposium on the Separation and Characterization of Natural and Synthetic

Macromolecules. Amsterdam, Netherlands, January 26-28, 2011

23. KCS 106th National Meeting & Exposition, Daegu EXCO, South Korea, October 14-15, 2010

24. Yonsei University 125th Anniversary International Symposium. Nano-biomolecular assembly, Yonsei

University, Seoul, South Korea, June 14-17, 2010

25. 105th National Meeting of the Korean Chemical Society, Songdo, South Korea, April 29-30, 2010

26. Green super rice-Hybrid rice seed production, NIBGE, Fsd, Pakistan, October 12-13, 2009

27. 2nd international seminar on analytical sciences, National Center of Excellence in analytical chemistry,

Jamshoro, Sindh University, Pakistan, September 6-8, 2007

Referees

1. Prof. Dr. Farooq Anwar (M.Sc. degree supervisor)

Professor of Analytical Chemistry, Department of Chemistry and Biochemistry, University of Sargodha,

Pakistan, E-mail: fqanwar@yahoo.com

2. Prof. Dr. Abdul Haque (Director Akhuwat FIRST)

Professor, Department of Biotechnology, Akhuwat FIRST, Faisalabad, Pakistan, E-mail: ahaqnibge@gmail.com

3. Prof. Dr. Han-Woong Lee (Ph.D. degree supervisors)

Professor of Biochemistry, Department of Biochemistry, Yonsei University, Sinchon-dong, Seodaemun-gu,

Seoul, 120-749 South Korea. E-mail: hwl@yonsei.ac.kr

4. Prof. Dr. Jaehwan Song (Ph.D. thesis committee member)

Biochemistry, Department of Biochemistry, Yonsei University, Sinchon-dong, Seodaemun-gu, Seoul, 120-

749 South Korea. E-mail: jso@yonsei.ac.kr

5. Prof. Dr. Myeong Hee Moon (M. Phil. degree supervisor)

Professor of Analytical and Bioanalytical Chemistry, Department of Chemistry, Yonsei University, Sinchon-

dong, Seodaemun-gu, Seoul, 120-749 South Korea. E-mail: mhmoon@yonsei.ac.kr

6. Dr. Yusuf Zafar (Chairman PARC)

Pakistan Agricultural Research Council, G-5, Islamabad - Pakistan, E-mail: y_zafar@yahoo.com

7. Dr. Shahid Mansoor (Head of research division at NIBGE and now Director NIBGE)

Chief scientist, ABD, NIBGE, Faisalabad, Pakistan, E-mail: smansoor@nibge.org