cscl preparation of plasmid dna biacore t200 =t Ü 4g c
TRANSCRIPT
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��• P2 • P8 • CsCl Preparation of Plasmid DNA P14� Biacore T200 14F P18 �
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BioMed Resource Core
1. R1438 & R1439
2. 9:00~17:00
3. ( ) 4. A. B. C. D.
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(1) (2) …(3) CFP GFP YFP RFP…(4) (5) siRNA miRNA(6) Adenoviral lentiviral retroviral vectors (7)
213(1) (2) …(3)
74 E.coli competent cell Staphylococcus Streptococcus
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Yeast two-hybrid cDNA library 9
310
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(gene construction)
stable clone
Yeast two hybrid assay
cDNA library
primary culture
cDNA library Genomic DNA library
mini- /midi- kit CsCl banding
BL21 (DE3) Baculovirus
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1. 2. 3. 4.
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Yeast Core Facility for Human Disease Model��
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USA, National BioResource Project/Japan)
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C. elegans Core Facility for Human Disease Model
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• Drosophila Core Facility for Human Disease Model
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NORCOMM etc.) ES
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(Conditional KO)
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Mouse Core Facility for Human Disease Model �
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• • strain 2000 • 500
• NT$ 600/strain
• RNAi knock down ( ) NT$ 800/clone or gene
�• 1 49 1 1 4 NT$ 4000�
• DNA chimera NT$ 200,000 per case �
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DNA(plasmid) DNA DNA
DNA DNA
DNA DNA
1998 DNA
”CsCl banding”
CsCl banding DNA
supercoiled form DNA
(transfection)
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� CsCl gradient & DNA banding :
CsCl (
1.91g/ml) Cs
Cs
Cs gradient
DNA CsCl
78000 rpm DNA
CsCl DNA
1.7g/ml 1.6g/ml RNA
1.8g/ml banding DNA
CsCl ultracentrifuge DNA
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� Ethidium Bromide & DNA banding
DNA DNA DNA
EtBr
AT/ GC
EtBr
(unwind) DNA
supercoiled form DNA linear form EtBr
EtBr
CsCl DNA
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