Parasite Risk Assessment with Integrated Tools 

in EU fish production value chains

PARASITE BPARASITE IN BRIEF(2013‐15)

8th Workshop for NRLs for Parasites Rome, 23‐24 May 2013

the EC launched a SPECIFIC CALL (First Time!!) for a better managing of this 

EMERGENT HAZARDEMERGENT HAZARD

THEME KBBE.2012.2.4-02

(Food safety and quality issuesy q y

related to parasites in seafood)

Funding scheme:  Collaborative Project

targeted to SMEs (30%)

What about the “Human fishery product‐borne parasitic diseases” in the EU context? (SoA) y p p

• The status quo 

about this hazard isabout this hazard is 

unsatisfactory!

•Many gaps have a y gaps a e

been identified 

between discovery 

and market up‐take!p

The PARASITE project will focus mainly onanisakid nematodes We have placed less emphasisanisakid nematodes. We have placed less emphasison trematodes and cestodes because, although zoonoticflatworms infect freshwater fish in many parts of theworld, consumption of freshwater fish in the EUis relatively low and localised.

The CRLP has reported that, in the EU, the only parasitestransmitted to humans through consumption of freshwater fish are thetrematode Opisthorchis felineus (responsible for around 180trematode Opisthorchis felineus (responsible for around 180infections due to consumption of marinated tench fillets, mainly inItaly, since 2003) and the cestode Diphyllobotrium latum (theetiological agent of about 80-90 infections per year in the EU due toetiological agent of about 80 90 infections per year in the EU due tothe consumption of raw or undercooked fillets of several perch andsalmon species, mainly in Estonia, Finland, France, Italy, Lithuania,Poland and Romania).Poland and Romania).

Fish products are AT RISKEconomic impact

Food security concern (industry)Food security concern (industry)

unfit for human consumptionhigh quality standard!

Consumer is AT RISKHealth impact Food safety concern (administration/consumer)Food safety concern (administration/consumer)

68

1012zoonoses allergy

024

2006

-120

06-3

2006

-520

06-7

2006

-920

06-1

120

07-1

2007

-320

07-5

2007

-720

07-9

2007

-11

2008

-120

08-3

2008

-520

08-7

2008

-920

08-1

120

09-1

2009

-320

09-5

2009

-720

09-9

We know the CONCERN…but there is no idea on the real dimension of this HAZARD

National Horizon Scanning (2010‐12): a Strategic Agenda with SHARED VISION 

http:// www.parcode.es

Management structurePARASITE Consortium 21 partners (15 RTDs; 6  SMEs)

12 countries (9 EC + 3 Asiatic region)

different background

Fish providing countries

TECHNET

different background

Nov, 2010International Symposium

P. no ORGANIZATION NAME SHORT NAME COUNTRY TYPE

1AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (COORDINATOR)

CSIC ES RTD

2 NATIONAL AND SEAFOOD RESEARCH NIFES NO RTD

3 UNIVERSITA DI TUSCIA-VITERBO UT-URS IT RTD

4 AGENCE NATIONALE DE SECURITE SANITAIRE ANSES FR RTD

5 CENTRO TECNOLOGICO DEL MAR - FUNDACION CETMAR CETMAR ES RTD

6 SERVICIO MADRILEÑO DE SALUD SERMAS ES RTD

7 HAVSTOVAN - FAROE MARINE RESEARCH INSTITUTE FAMRI FO RTD

8 ISTITUTO SUPERIORE DI SANITA ISS IT RTD8 ISTITUTO SUPERIORE DI SANITA ISS IT RTD

9 INSTITUTE FOR BIOTECHNOLOGY AND ENVIRONMENT, IBE VN RTD

10 ZEIJHAN OCEAN UNIVERSITY OF CHINA ZOUC CC RTD

11 CENTRAL LUZON STATE UNIVERSITY CLSU FL RTD11 CENTRAL LUZON STATE UNIVERSITY CLSU FL RTD

12 MAX RUBNER-INSTITUT MRI DE RTD

13 UNIVERSITY OF COPENHAGEN UOC DK RTD

14 INSTITUT ZA OCEANOGRAFIJU I RIBARSTVO ( AND FISHERIES) IZOR CR RTD

15 UNIVERSITY OF ABERDEEN UNIABDN UK RTD

16 LARPRO ENGINEERING, S.L. LARPRO ES SME

17 COOPERATIVA DE ARMADORES DE PESCA DEL PUERTO DE VIGO ARVI ES SME

18 COMERCIAL HOSPITALARIA GRUPO 3, S.L. CHG3 ES SME

19 TECHNET, S.A. TNET DE SME

20 HERMES, S.A. HERMES NO SME

21 NEDERLOF’S VISHANDEL B.V. NEDERLOF´S NL SME

Risk Analysis (GMPs in 80’s, HACCP in 90’s)

WP2Exposure

AssessmentRisk Assessment

Targeted End Users

WP4H d

WP9Risk

CommunicationWP1

Monitoring

Hazardidentification

CommunicationProject Management

Knowledge  transferForeground‐dissemination

WP5Hazard

characterization

WP8Risk

WP3Sample and

Datamanagement

g

characterizationAnalysis

Traslational R h M d l

WP6Improvement of

detection methods

WP7Interventions

in thefood chainIntegrated Tools

Research Modelswith a best‐value for money approach

Mitigation

Detection

Key performance topics (EFSA)y p p• Surveillance and monitoring in Fish species/Fishing areas/Production systems

Objectives:

1 to identify and characterise species and populations of1. to identify and characterise species and populations ofzoonotic nematode parasites infecting seafood fromdifferent areas.

2. To develop new genetic markers for genotyping Anisakisspecies

• Freshly imported fillets of Nile perch to Germany.• Imported squid to Spain

p3. To establish genes and design primers/probes to be used

as “DNA barcodes”.4. To gather genetic variability data of parasites populations

to be correlated to their infestation levels in order tot bli h i tifi b f l l id i l i l• Imported squid to Spain.

• Fresh or smoked products of tuna on French and Italian markets.• Vietnamese Pangasius production systems.

establish scientific bases for molecular epidemiologicalstudies of each parasite species and their populations indifferent geographical areas.

Key performance topics (EFSA)y p p• Diagnostic awareness of allergic reactions and epidemiological studies

Objectives:

1. To determine if any other anisakid apart from Anisakis spp.that can infect the muscle of fish

have allergenic capacity are able to induce sensitization after oral administration, whether

untreated or heat-treated are specifically recognized by antibodies presented in sera from

fish-eating peoplefish-eating people2. To detect Anisakis spp. allergens in fishery products

(proteomics) and from different regions.4. To characterize cellular and humoral immune responses to

anisakid antigens

Key performance topics (EFSA)y p p• Improvement of detection methods for the industry

Improvement of the visualinspection scheme for detectionby the press-method(hyperespectral computing)

Implementation of molecularmethodology based on RealTime-PCR to detect parasitesand/or their traces in fishery

MALDI-TOF or nano-liquidchromatography connected toion trap mass spectrometry

products.

Ring Trial involving at least five experienced

ANIMALS DNAPROTEINS

Ring Trial involving at least five experiencedlaboratories to evaluate reproducibility of the test/s andreliability of data produced by each laboratory

Beta-testing of validated detection methods at industrial level

Key performance topics (EFSA)Key performance topics (EFSA)• Interventions in the food web to inactivate parasites

at sea At sea on siteon site

Device to kill zoonoticnematodes in offalsonboard

at sea

Application of specific treatments toreduce or inactivate the allergeniccapacit

At sea

Inactivation under novel freezing systems(PSF, CAS), and other treatments like highhydrostatic pressure low voltage currentonboard.

technological solution formanagement of parasitecontaminants in residuesunder the Reglaments

capacity.strategies to eliminate the allergens (e.g.selective precipitations) or reduce theiractivity (e.g. by crosslinking to otherproteins present such as in industrial

hydrostatic pressure, low voltage current,modified atmosphere packaging,electrolyzed-oxidizing water, radiofrequency,ultrasonic waves.Optimization of the conditions in terms of

1069/2009 and 142/2011(Animal by-productsRegulations).

processes used to elaborate surimi gels).Efficiency of the selected treatments will beassessed by immunoblotting usingsensitized-patient’s sera.

quality of the product will be evaluate byVibrational spectroscopy (Fourier Transform(FT)-infrared and FT-Raman) and Low FieldNuclear Magnetic Resonance (LF-NMR)

Key performance topics (EFSA)ey pe o a ce topics (E SA)•Integration of “intelligent” information (emergent, structured, targeted)

Cost / Benefitscenarios on theapplication of treatments and

Quantitative riskassessment, with aparticular focus on the probability

Model consumerwillingness to pay fortreatments to reduce the application of treatments and

tools for policymakers andfood producers. In eachscenario the applicableparasite elimination method orrisk evaluation tool will be

of illness from parasitesassociated with the consumptionof raw or partially cooked seafoodproducts and estimating theeffectiveness of proposed risk

incidence of parasites in fishproducts. The new data onconsumer preferences will be derivedfrom a survey of consumers. We

risk evaluation tool will beanalysed with regard to itsdegree of applicability andeffectiveness, vs. cost,comparing to tools and

effectiveness of proposed riskmitigation strategies.

A process based risk assessmentmodel will be developed using

@RISK ft

envisage 500-800 individuals to besurveyed (quota survey) in eachparticipant country for the generationof the data set to be used for thestatistical analysis, which will follow a

methods used currently.

Border controlauthorities and

@RISK software. Themodel will include variability anduncertainty in input data andsimulation will be carried out usingthe Monte Carlo technique The

y ,

multinomialregression-basedmethodology. authorities and

food producerswill be invited to participate, inorder to verify the data usedfor the definition and

the Monte Carlo technique. Themodel will be parameterised for twoareas within the EU, one with high(Spain) and one with low (UK) levelsof human disease incidence.

methodology.

for the definition andevaluation.

PARASITE is aware of the need to be outcome focused.PARASITE is aware of the need to be outcome focused.Connected across the institutional spectrum and socially embedded

DELIVERABLES (34: 50% PU): Risk Assessment (56%): evidence‐based (high quality robust results)

Integrated Tools (20%) marketed aligned (technology push vs. market pull).

Exploitation Plan (24%): IPR valorization instruments

OTHER DISSEMINATION PRODUCTS  Catalogue of technological results

Training workshops (diagnostic tools and operating strategies)

Sh t t t (i d t d i i t ti t ff) Short‐term stages (industry, administration staff)

Work with media professionals (discussion panels)

Specific events (e g 9 ISFP 2015) Specific events (e.g., 9 ISFP 2015)

WEBSITE...

http://parasite-project.eup p p j

The innovation dimension of PARASITE is under the

criterion of IMPACT that means social usefulness of itscriterion of IMPACT, that means social‐usefulness of its

research findings by:

1/ h i th “E dd d l ” f hi h1/ enhancing the “European added value”: safe, high 

quality seafood

2/ strengthening the competitiveness of “European food2/ strengthening the competitiveness of “European food 

producers and markets”

3/ improving “European food safety policies”3/ improving  European food safety policies  

Project Governation:j Consortium Agreement

Grant Agreement

Culture of openness and debate

Consensus model (SC, EDC, EAC)

Road‐mapping the future prospects…

EU ‐ Fish dependence EU fishing industry the fourth largest in the world (6.4 million tons/year)g y g ( /y ) Processing industry 4000 companies (SMEs) and 350,000 peopleWorld‐class fish consumer (22.3 kg; up to 55) Increased International trade (60% of its fish consumption: “Finfish Study 2011”) Increased International trade (60% of its fish consumption:  Finfish Study 2011 )

…a plea for a future collaborative work of the PARASITE network with

CRLP and NRLs in relation to marine parasites with economic andCRLP and NRLs in relation to marine parasites with economic and

public health concern

Visible parasites f i d t i lVisible parasites of industrial concernLISTING OF FLESH PARASITES IN FISH SPECIES, FISH PRODUCTS AND 

SUBPRODUCTSSUBPRODUCTS

“The dirty dozen” PARASITE ARRAY in seafoody f

Microparasites

Mixosporidians (Kudoa spp.) Microsporidians (Pleistophora sp., Spraguea sp.) Flagelates (Giardia spp.) A i l (C idi ) Apicomplexa (Cryptosporidium spp.)

Macroparasites

Trematodes (Didymozoidae) Cestodes (Gymnorynchus sp., Molicola spp., Maccalumtrema xiphiados)  Nematodes (Anisakis spp Pseudoterranova spp ) Nematodes (Anisakis spp., Pseudoterranova spp.) Crustacea (Pennella spp.,…)

Santiago Pascuall ii i Thank you!spascual@iim.csic.es

http://parasite‐project.eu