cpc: classification of renal glomerulonephritis glomerular ... · glomerular proliferation 1....
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C PC :G lom eru lonephrit is G era ld B A ppe l, M D V ivette D ’A gati, M D
C lass ification o f Rena lG lom eru la r D iseases
• M orpho log ica l• Im m uno log ica l• Etio log ica l• C lin ica l
V u lne rab ility o f G lom eru lusto IC In ju ry
1 . 20 -25% Card iac O utput2 . H igh g lom eru la r cap illa ry p ressu re3 . Fenestrated endothe lium4 . Concentrat ion (s iev ing e ffect)
M echan ism s o f Im m uno log icIn ju ry to the G lom eru lus
1 . G lom eru la r depos it ion o fc ircu lat ing A g-A b com p lexes
2 . B ind ing o f C ircu lat ing A b tostructu ra l g lom eru la r A g ( i.e . anti-GBM A b )
3 . In s itu im m une com p lex fo rm ation
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G lom eru la r P ro life rat ion
1 . Endocap illa ry
2 . Extracap illa ry(c rescentic )
Patte rns o f G lom eru la r D isease
G loba lV s2 .Segm enta l
D iffuseV s1 . Foca l
Signs of GlomerularDisease
Erythrocyte Casts
Deformed-Crenated Urinary RBC’s
Large amounts Albuminuria
( >3g/D )
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• 7 y o W M c/o x severa l days bad so reth roat + low g rade tem peratu re ; he isg iven acetam inophen , and recoversuneventfu lly . 2 w ks late r deve lops dark ,coca-co la co lo red u rine and notesu rinat ing less . O n P x peda l edem a andan e levated b lood p ressu re .
• Labs:– U/A rbc’s , rbc casts , 2+ p rot .– C reatin ine 2 .4 m g/d l– Com p lem ent 22 (no rm a l 50 -150)– C3 leve l low– A SLO 1250 (no rm a l < 250)
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N ephrit ic Synd rom e
• D ecreased GFR
• O ligu ria
• Edem a
• Hypertens ion
• A ctive u rinary sed im ent
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6
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Post-S treptococca l GN
• Fo llow s certa in se ro type streptococca lin fections – so re th roats , im petigo , etc .
• Ch ild ren m ore com m on than adu lts• T im e lag betw een in fection & k idney
d isease• N ephrit ic p ictu re com m on• Sero log ic tests fo r s trept in fections +• Low com p lem ent and C3 leve ls• Exce llent p rognos is ch ild ren , + /- in adu lts
Serum Complement in GN
• Low Levels Post-infectious GN SLE Cryoglobulinemia
Idiopathic MPGN
• Normal Levels MCD, FSGS, Memb Neph, Amyloidosis, IgA, DM, ANCA + RPGN, Goodpastre’s, HSP, etc.
• A 16 y o h igh schoo l jun io r noticesdark b row n u rine a fte r p lay ingbasketba ll. U rinary sed im ent hasrbc ’s and rbc casts .
• Labs:– C reatin ine 1 .1 m g/d l– C reatin ine c learance 128 cc/m in– 660 m g p rote inu ria/day– Sero log ic tests a re no rm a l o r
negative
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D em ograph ics o f IgAN ephropathy
A ges 4 – 80 (m ean 25 ) years(65% o f patients in 2 n d/3 rd decade)
M /F = 2/1Rare in b lacks
Inc idence (% p rim ary g lom eru lopath ies)5 -10% N . A m erica
U .K .S cand inav ia
20 -30% EuropeA ustra lia
25 -45% A sia
C lass ification
• Prim ary– IgA N ephropathy– Henoch-S chon le in Pu rpura
• Secondary– L ive r C irrhos is– Inflam m ato ry Bow e l D isease
Pathogenes is
1 . D efective hepatic c learance– L ive r c irrhos is
2 . Increased IgA p roduction– A ssoc iat ion w ith e levated se rum IgA– O nset m ay fo llow UR I o r Gastroenterit is
3 . D efect o f antigen exc lus ion at the m ucosa l su rface
– UR I– Gastroenterit is– Ce liac d isease
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S tructu re o f Hum anSecreto ry IgA (s igA )
IgA Nephropathy
• M ost com m on id iopath ic GN in w orld• D efined by IgA depos it ion in
m esang ium• Presents- Young – g ross hem aturia A du lts – P ro te inu ria +
hem aturia• N ot ben ign hem aturia ( Berger’s D is
)• 20 -30 % p rog ress ESRD over 20
years• Rx – A CE inh ib . + S tds , F .O ., M M F
Cort icoste ro ids in IgA N : acontro lled tria l
86 Pts U p rot 1 -3 .5g/D P creat < 1 .5 m g/d lRx cyc lic Pu lse SM + Q O D stds v s PBO x 6 m o .Endpo int 50% rise in P creat. Fo llow 6 y rs
Endpo int 9 /43 Rx vs . 14/43 PBO ( p< .05 )H igh r isk P ts : vascu la r sc le ros is , m a les , no S te ro id RxN o m ajo r s ide e ffects
Pozz i e t a l. Lancet 353 :883 , 1999
IgA N ephropathy : AContro lled T ria l o f S te ro ids
(Pozz i, e t a l)
Contro lled T ria l o f F ish O ils inIgA N
106 Pts 78M /28F age 36yo U p rot > 1 g/D HBP 60%Rx M ax EPA 12g/D ( 58 ) v s O live o il ( 51
)Rx 2yr fo llow 5 y r Endpo int 50% increase P creat.
Endpo int 6% Rx EPA v s 3 3%PBO
Change Pcreat .03 m g/d l v s .14m g/d l
D D T 10% v s40%
D onad io et a l N Eng J M ed1994
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Im m unosuppress ive Rx fo r IgA NChange in P ro te inu ria
Ba lla rd ie , FW , Roberts , ID . J A m Soc N eph, 13 :142 -148 , 2002 .
• A 29 y o sa lesw om an deve lops a rth rit iso f m u lt ip le jo in ts , fever,lym phadenopathy, and a m a la r rash .
• Labs:– U rina lys is 3+ p rote in , c renated rbc ’s– C reatin ine 1 .2 m g/d l– 24 h r. p ro te in 1 .8 g/d l– Com p lem ent 18% (no rm a l 50 -150% )– A N A pos it ive , A nti-D N A antibody pos it ive
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Lupus N ephrit is C lass IV
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Lupus N ephrit is W HO C lass ification
CLA SSESI M in im a l m esang ia lII M esang ia l P ro life rat iveIII Foca l Segm enta l P ro life rat iveIV D iffuse P ro life rat iveV M em branous
Lupus N ephrit is C lass II
Treatment of Lupus Nephritisby Class
• Class I and II – Treat extra-renal findings
• Class III -FPLN – Vigorous Rx if necrotizingfeatures, crescents, extensiveproliferation.
• Class IV – DPLN – Vigorous Rximmunosuppressives
• Class V – Memb LN – Treat to induce remitproteinuria – Nephrotic syndrome
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Predictors of Progression of LupusNephritis in Three Ethnic Groups
N ew Yo rk C ity Cohort:129 pts -51 H , 22 A A , 55 C C lass III - IVLNPred icto rs (age-ad justed hazard rat io )
H ispan ic ethn ic ity (3 .7 )A frican – A m erican race (3 .1 )L iv ing in ne ighborhood w ith h igh poverty (2 .9 )Governm ent insu rance – M ed icare (3 .2 )E levated c reatin ine (4 .3 )P rote inu ria (3 .8 )Hypertens ion (3 .2 )W HO C lass IV (3 .3 ) Barr… A ppe l e t a l,2003
Impact of Race on RenalPrognosis – NYC n= 129
Im pact o f Poverty on Rena lP rognos is- N YC
Probab ility o f D eve lop ing End -Stage Rena lD isease : Com parison A m ong Lupus N ephrit is
T reatm ent Reg im ens
CYC = cyc lophospham ide ; A ZA = azath iop rine .S te inberg A D , S te inberg SC . A rth rit is Rheum . 1991 ;34 :945 -950 .
M onths
Probab ilityo f
End -StageRena l
D isease
IV CYCO ra l CYC + A ZA
O ra l CYC
A ZA
Predn isone
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M u lt icenter T ria l o f M M F vs IV Cycfo r Induction Therapy o f Severe
LN• M u lt icenter, random ized , nonb linded tria l
o f induction RX fo r severe active LN• D es igned as equ iva lence tria l
• Ca lcu lated sam p le s ize : 64/ Rx a rm• Hypothes is : M M F has equ iva lent e fficacy
w ith superio r tox ic ity/to le rab ility p rofi levs. IV C
A CR G inz le r et a l 2003 , A SN A ppe l e t a l 2003
Base line Patient Characte rist ics
33 .2 ± 115 .510 .3 ± 17 .3
24 .1 ± 50 .312 .6 ± 23 .5
U rine sed im entRBC/hpfW BC/hpf
4 .41 ± 3 .514 .06 ± 3 .14U rine p ro te in , g/24 h r1 .08 ± 0 .491 .06 ± 0 .52Screatin ine, m g/dL58 .70 ±80 .64
43 .72 ±66 .88
D uration o f SLE , m o .36 (52% )43 (61% )B lack
2 .69 ± 0 .562 .81 ± 0 .95Sa lbum in , g/L
65 (94% )61 (86% )Fem a le31 .0 ± 9 .032 .5 ± 10 .0A ge ( y rs)IV C (n=69)M M F
(n=71)
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W HO Rena l B iopsy C lass ificationo f S tudy Popu lat ion
8 7M ixed 13 14M em branous ( V )
37 11
39 11
Pro life rat ive C lass IV C lass III
IV C(n=69)
M M F(n=71)
Rem iss ion Rates: M M F vs . IV C
16/71
4/69
21/71
17/69
37/71
21/69
In tent-to -T reatA na lys is
P=N SP=0 .005
P=0 .009
Perc
ent
Resp
ondi
ng
C hange in P redn isone D ose
P = 0 .064
Pred
niso
ne (
mg/
day)
C hange in Serum C reatin ineand
U rine P ro te in ExcretionSerum
Creatin ineU rine
P rote in
Seru
m C
reat
inin
e (m
g/d
L)
Uri
ne P
rote
in (
mg/
dL)
C hange in U rine Sed im entRBC W BC
M M F IV C
RBC
/hpf
WB
C/h
pf
C hange in Com p lem entCom ponents
C3 C4
M M F IV C
C3
(m
g/dL
)
C4
(m
g/dL
)
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C hange in A nti-d sD N A andSerum A lbum in
A nti-d sD N A Serum A lbum in
Ant
i-dsD
NA
sco
re
Seru
m A
lbum
in (
mg/
dL)
MMF vs IVCY Induction - 24Wk Remission Rates: AA vs Others
Com p lete Rem iss ionM M F B lackM M F O therIV CY B lackIV CY O therCom p lete + Part ia lM M F B lackM M F O therIV CY B lackIV CY O ther
A ppe l e t a l, A SN 2003
• A 58 y o insu rance sa lesm an deve lopss inus it is , w e ight loss , m a la ise and a d rycough over th ree w eeks. H is s inus fi lm sshow o pac ification o f the le ft m ax illa rys inus, and he is found to have a cav ita ryles ion on h is chest X -ray .
• Labs:– U rina lys is : rbc’s , w bc ’s , and rbc casts– C reatin ine 2 .7 m g/d l– Serum com p lem ent is no rm a l– A nti-GBM antibod ies a re absent– A N CA is pos it ive
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Pu lm onary -Rena l V ascu lit icSyndrom e
• Pauc i- im m une (usua lly A N CA -assoc iated)
– W egener’s g ranu lom atos is– M icroscop ic P o lyang iit is
• Im m une Com p lex D epos its (g ranu la r)– SLE– C ryog lobu linem ic v ascu lit is
• A nti-G lom eru la r Basem ent M em braneA ntibody D epos its ( linear)
– Goodpastu re ’s Syndrom e
C ircu lat ing a nti-GBMantibod ies w ith linearg lom eru la r IF sta in ing
C ircu lat ing A N CA w ith pauc ity o f g lom eru la r IFim m unog lobu lin sta in ing
G lom eru la r im m unecom p lex loca lizat ion w ith
g ranu la r IF sta in ing
A N T IBO D Y M ED IA TED GLO M ERULO N EPHR IT IS
>80% A N CA +
2 0
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R ap id ly P rog ress iveG lom eru lonephrit is
A severe fo rm o f GN lead ing to RF in daysto m onths
RPGN = C rescentic GNSecondary RPGN ( SLE , HSP , Post-
in fectious, e tc . )P rim ary RPGN - anti-GBM d isease - im m une com p lex
GN - pauc i- im m une GNRx and Course depend on et io logy and
stage
T reatm ent o f RPGN
• A nti-GBM d isease – S te ro ids ,cytotox ics , and p lasm apheres is
• Im m une Com p lex GN – T reatunderly ing d isease
• Pauc i- im m une RPGN ( A N CA + ) –Cytotox ics ( Iv o r P .O . )
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A nti-N eutroph il C ytop lasm icA ntibod ies
• C -A N CA cytop lasm ic aga inst se rine p ro te inase 3 ( PR3 )• P-A N CA perinuc lear aga inst m ye loperox idase ( M PO )• P-A N CA is an a rt ifact o f a lcoho l
fixation
A N CA is to RPGN as A nti-D N A is toSLE
Rena l Pu lm onary Syndrom es
• Goodpastu re ’s Synd . A nti GBM A bs• SLE lung d is . + LN aD N A + CH50• RPGN , W eg.G ., PA N A N CA•• Pu lm onary em bo li RV T ( m em b N S
)• Pneum on ia Im m une
com p lex GN• U rem ic Lung CHF + Rena l
fa ilu re