Core-Shell Optimization Kit (PN: AQ0-8892)

• Increasemethodefficiency,resolution,anddetection

• Improveresultsforsensitiveanddemandingapplications

o Minimizedeadvolumebetweeninjectoranddetector

o Facilitatequickandreliableconnections

o Createsmallvolumepeaksthatcanbedefinedathigh detectoracquisitionrates

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ThisCore-ShellOptimizationKitinstallationguideoutlinesafewchanges thatshouldbemadetoyourcurrentHPLCsystemtoprovideUHPLC-like performanceimprovements.

Thefittingsandtubingusedinthiskitarecarefullychosentominimizedead volumeandreducebandbroadening.CombinedwithyourCore-ShellcolumnandacolumnprotectionsystemsuchasSecurityGuard™ULTra,thiskitwillprovidereliableconnectionsandqualityperformanceeverytime.

KIT COMPONENTS

Description Kit UOM

PEEKsil™Tubing0.100mmIDx¹/16in. ODx20cmL,red 2/Pk

PEEKsil™Tubing0.100mmIDx¹/16in. ODx10cmL,red Ea

Sure-Lok™HighPressurePEEK™1-PcMaleNutFitting, 10-32,For¹/16inTubing 10/Pk

Sure-Lok™MaleNutFittingTighteningTool,aluminum Ea

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Injector

F L O W

Pre-Heater

SecurityGuard ULTRAColumn Protection System

UHPLC Column

Detector(≥ 40 Hz)

Installing Your Core-Shell Optimization Kit

PEEKsil Tubing20 cm (200 mm)

PEEKsil Tubing10 cm (100 mm)

PEEKsil Tubing20 cm (200 mm)

UHPLC Column Protection

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INSTallINg yOUr COrE-ShEll OPTIMIzaTION KIT:

1. Inspectthe¹/16inPEEKsil™tubingpiecestoensurethetubingiscleananddefect-free.PEEKsiltubingisprecisionpre-cutandshouldnotbecutagain.Ifadditionaltubingisneededorshorterlengthsaredesired,replace-menttubingisavailable.SeeOrderingInformationonpage5.

2. Itisrecommendedthatkitconnectionsbemadethroughyoursysteminthesamedirectionastheflow,i.e.,beginattheinjectorandcompleteyourconnectionsatthedetectorend.

3. Selecttheproperlengthoftubingfortheconnectionbetweentheinjectorandthecolumn,orbetweeninjectorandcolumnpre-heater,ifapre-heaterispresent.a200mmlengthisrecommendedforthisconnection.

4. InspecteachSure-Lok™PEEKmalenutfittingbeforeuse.

a.Note:–Beforere-useinspectandreplacethenutifthreadcompression and/orwearisobserved.

5. SlideoneSure-LokPEEKmalenutfittingontothetubingandinsertthetubingendwiththefittingintotheinjectorport.Seatthetubingallthewaydownintotheinjectorport.Thetubeshouldbecenteredandpushedintotheportuntilitbottomsout.

6. Whileholdingthetubinginplace,turn(screwclockwise)thePEEKmalenutfittingbyhandasfarasitwillgo.Seating(sealing)offittingsismadebytheincreasedfrictionfromcompression.asecureconnectionusuallytakesonlyabouta¼turnbeyondthepointwherethefittingferrulefirststartstograbthetubing.

IMPOrTaNT:Forapplicationsover3000psi(207bar),itisimperativethatthealumi-mumTighteningTool(aQ0-8530),includedinthiskit,beusedtopreventthetubingfromleakingorslipping.Tightenthenutabout¼to½turn,butdonotovertighten.

Note:Ifyouexperienceleaking,tightenanother¼turn.

7. SlideasecondPEEKmalenutfittingontothetubingandcompletethe connectiontoyourguardcolumn(seeFigure1),column,orpre-heater,repeatingthepositioningandtighteningsteps(5and6)above.

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8. Ifacolumnpre-heaterispresentwithcompatiblefittings,usethe100mmofPEEKsiltubingandtwoPEEKmalenutfittingstomaketheconnectionsbe-tweenthepre-heaterandtheinletofyourguardcolumnorCore-Shellcolumn.

9. Lastly,usingthesecondpieceof200mmtubingandtwomorePEEKmale nutfittings,maketheconnectionbetweentheoutletofthecolumnandthe detector,inthesamemannerthattheconnectionbetweeninjectorandcolumnismade.

Congratulations! TheinstallationofyourCore-ShellOptimizationKitisnowcomplete!

COMPONENT rEOrDErINg INfOrMaTION:

Description Kit UOM replacement Part No.

PEEKsilTubing0.100mmIDx¹/16in. ODx20cmL,red 2/Pk aT0-8896(5/Pk)

PEEKsilTubing0.100mmIDx¹/16in. ODx10cmL,red Ea aT0-8897(5/Pk)

Sure-LokHighPressurePEEK1-PcMale NutFitting,10-32,For¹/16inTubing 10/Pk aQ0-8503(10/Pk)

Sure-LokMaleNutFittingTighteningTool, aluminum Ea aQ0-8530(Ea)

Figure1.ConnectingPEEKsilTubingwithPEEKmalenutfittingtoGuardColumn

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aDDITIONal TIPS TO aChIEvE aND KEEP OPTIMal UhPlC PErfOrMaNCE ON yOUr hPlC

Tooptimizeyourseparation,werecommendthefollowing:

Sample• Minimizesampleinjectionvolumes(uselow-volumesyringes)• Filterallsampleswitha0.20µmPhenexsyringefilter (seedetailsonpages10-11).

• MakesamplediluentstrengthweakerthanthemobilephaseMicroparticulates• Filtermobilephasesolventsbeforeuse• Makefreshmobilephaseoften• Performroutinepumppistonsealmaintenance(washingandregularreplacement)• replacepurgevalvePTFEfritsperiodically(monitorbaselinebackpressure forincreases)

• PerformroutineinjectionvalverotormaintenanceInjector • Uselowdispersioninjectionvalvesandloops• Useasmall-volumeinjectorneedleseat• UseweaksolventinjectionsorgradientsColumn• Selectandoptimizethecolumnformaximumpeakresolution(ruggedness)• Optimizecriticalpeaksawayfromearlyelution• reducecolumnlengthand/orIDtoshortenruntime• reduceflowratetoreducebackpressureifneeded• Usealow-volumein-linefilterorguardsystemtoprotectthecolumnsuchas SecurityGuardULTracolumnprotectionsystem

• MatchpackedguardmaterialstothecolumnI.D.andphase• Employtemperaturecontrol(includingamobilephasepre-heaterbeforethe column)

Connections, Tubing and fittings• Maketight,optimizedconnectionsatallpoints,betweeninjector,column anddetector

• Usesmalldiametertubing,micro-volumefittingsandunions• Usepre-cutPEEKsil™tubingwhentubingdiametersarelessthan0.005in/0.127mmDetector• Minimizevolumeoftheassociatedheatexchanger• Useoptimizeddetectorcellgeometry(includeslengthandradius)• Decreasedetectortimeconstant(useafasteracquisitionrate)

o Forbestresultswhenusingcore-shellcolumns,collectdataatarateof 40Hzormore.

Formorein-depthtipsandhintsonoptimizingyourHPLC, visitwww.phenomenex.com/verify

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PEEKsil™ TUbINg

• Minimizes extra-column effects and band broadening• Precision-cut,ultra-squaretubeends• Exceptionallysmoothinnersurfaces

PEEKsilispolymer-sheathedfusedsilicatubing.ThePEEK™portionismechani-callystrongandhasidealsealingcharacteristicswhenusedwithconventionalmetalorPEEKferrulesystems.Thetubinginthiskitiscapableofwithstand-inghighpressuresupto10,000psi(689bar).Theexceptionallysmoothinnersurfacesarefreeoftheimperfectionscommoninsteeltubing.Thislessensthepossibilityofpathblockages,whilealsoultimatelyreducingbandbroadening.Theprecision-cut,ultra-squareandsmoothtubeendsenableoptimallowvolumeconnectionstobemade,whichwillimproveoverallchromatographicperfor-mance.Forhigherefficienciesandimprovedresolution,PEEKsiltubingisrecom-mendedtohelpoptimizeyourHPLC/UHPLCsystem.

Notes:PEEKsiliscompatiblewithmostorganicsolventsandhasaneffectivepHrangefrom0to10.

Polymide

Fused Silica Lining

Polymer (PEEK)Outer Sheath

0 ID

ID

Figure2.PEEKsiltubingcut-away

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UhPlC / hPlC SUrE-lOK™ PEEK MalE NUT fITTINg

•Pressure-ratedto10,000psi(689bar)whenusedwithyourCore-Shell Optimization Kit

•Sealswithouttheneedforwrenches

MadeofaproprietaryPEEKblend,theseultra-highperfor-mancepolymericfittingsaresuitableforallbutthemostextremehigh-pressureapplications,andbestforion-andbio-chromatography.Theseuniversal,one-piecemalenutfittingwillholdany¹/16indiameterPEEK,stainlessortita-niumtubing.Usethetighteningtool(aQ0-8530,seepage9)toeasilysecure yourconnectionsinordertoavoidleaksandtubingslippage.

IMPOrTaNT!althoughthekitmalenutfittingsarepressureratedto12,000psi(827bar),thekittubingispressureratedtoonly10,000psi(689bar).Therefore,whenusedwiththekittubing,thesefittingsCaNNOTbeusedatpressureshigherthantheratingofthetubingitself.allfittingsdohavelimitedlifetimes.Phenomenex recommendsinspectingthethreadsbeforere-useandreplacingyourfittings aftermultipletighteningcyclesorwheneverthreadcompressionand/orwearis observed.

aQ0-8503

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PEEK MalE NUT fITTINg TOOl

• Quick and easy tightening of PEEK fittings

Usethishandytooltotightenanystandard,short-orlong-styleknurl-headedPEEKmalenutfittingslike aQ0-8503.Thetoolcanalsobeusedwithmanyofthelow-pressurenutscommonlyusedinthelab.

Quick Use Notes:Push/slidethenarrowendofthetoolallthewaydownontotheheadofthenut.Iftheconnectionisbeingmadetothecolumnside,useawrenchonthecolumnfittingtoholditfrommoving,whileusingthefittingtool toturnthenutclockwiseuntiltightened.Slidethetooloffthenuttocomplete theinstallation.

Note:Thetorqueachievedwilldependonthepolymerconstructionofthe nut,thedegreeofnutheadengagement,aswellasnormalmanufacturing tolerances.

SaMPlE fIlTraTION: Phenex™ Syringe filters

WhenusingsmallerparticleHPLC/UHPLCcolumnswithlow-volumeconnections,itisessentialthatmicroparticulatesberemovedfirstbypre-filtration.Phenex™syringefilterseffectivelyremoveunwantedsampleparticulatesbeforetheycanenterthesystemflowpath,causingblockedinjectors,tubing,orcloggedcolumnsthatdegradeperformance.Pre-filteringwillextendyourcolumn’slifetime,increasesystemuptime,andpreventunnecessarytroubleshootingandrework.

ForaqueousorMixedOrganicSolutions,usePhenex-rC(regenerated Cellulose)syringefilters.For100%OrganicSolutions,usePhenex-PTFE(Poly-tetrafluoroethylene)syringefilters.Forcolumnspackedwith≤4μmchromato-graphicmedia,asyringefilterwith0.20μmfiltermembraneisrecommended. Forcolumnspackedwith≥5μmchromatographicmedia,asyringefilterwith0.45μmfiltermembraneisrecommended.

Seeproductselectionandorderinginformationonpage10-11

aQ0-8503

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Selection Guide

1. chooSe filter diameter baSed on Sample volume

UhPlC/hPlC SaMPlE fIlTraTION

4 mm Diameter 15 Diameter 25-28 mm Diameter≤ 2 mL Sample Volume 2 - 10 mL Sample Volume 10 - 100 mL Sample Volume

PhenexSyringeFilterOrderingInformation

4 mm diameterfor ≤ 2 ml sample volumes

15 mm diameterfor 2 - 10 ml sample volumes

25 -28 mm diameterfor 10 - 100 ml sample volumes

membrane type/Size

part no. unit part no. unit part no. unit

0.20 µm phenex-rc(Regenerated Cellulose)

AF0-3203-12 100/Pk AF0-2203-12 100/Pk AF0-8203-12 4 100/Pk

AF0-3203-52 500/Pk AF0-2203-52 500/Pk AF0-8203-52 4 500/Pk

phenex-peS 2

(Polyethersulfone)— — — — AF0-8208-12 6 100/Pk

— — — — AF0-8208-52 6 500/Pk

phenex-ptfe 5

(Polytetrafluoroeth-ylene)

AF0-3202-12 100/Pk AF0-2202-12 100/Pk AF0-1202-12 100/Pk

AF0-3202-52 500/Pk AF0-2202-52 500/Pk AF0-1202-52 500/Pk

phenex-nY(Nylon)

AF3-3207-12 100/Pk AF0-2207-12 100/Pk AF0-1207-12 100/Pk

AF3-3207-52 500/Pk AF0-2207-52 500/Pk AF0-1207-52 500/Pk

phenex-Gf/nY(Glass Fiber/Nylon)

1.20 µmphenex-Gf 1,2

(Glass Fiber)Prefiltration of heavily contaminated or highly viscous samples. When used in-line preceding a 0.20 μm membrane filter, clog-ging of the membrane filter is prevented and sample clean up is

AF0-8515-12 6 100/Pk

AF0-8515-52 6 500/Pk

Above syringe filters are non-sterile. Housing is made of medical-grade polypropylene (PP) and offer luer lock inlet/slip outlet connections, unless otherwise indicated.

An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a Nylon (NY) membrane. Excellent for filtration of particle-laden samples, such as foods and beverages, environmental, biofuels, and dissolution samples. Use less hand pressure to filter even the most difficult samples. Outlet connection is luer-lock.

AF0-1A47-12 AF0-1A47-52

100/pk500/pk

PhENEx™ SyrINgE fIlTErS

• rapid filtration of hPlC and gC samples prior to analysis• Particulate,PVCandextractable-freefilters• Moreconsistent,reliableperformance

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whY Select 0.20 µm SYrinGe filterS?Forcolumnspackedwith≤3µmchromatographicmedia,asyringefilterwith0.20µmfiltermembraneisrecommended.Phenex0.20µmsyringefiltersprovideaneffectivebarrieragainstunwantedparticulatesfromenteringthesystemflowpath.Thisreducescolumnpluggingleadingtolongercolumnlifetimesandincreasedsystemuptime.

2. chooSe filter membrane accordinG to the characteriSticS of Your Sample and filterinG objective

membrane type / recommended uses

rc regeneratedCellulose(rC)-HydrophilicregeneratedCellulosefiltermembranesarecompatiblewithaverybroadrangeofaqueousandmixed-organicsolutions,makingthemoneofthemostuniversalfiltermaterialspriortochromatography.Phenex-rCfiltersalsoexhibitfast-flowandultra-lowproteinandnon-specificbindingcharacteristics.Duetothebeneficialmaterialcharacteristics,Phenex-rCmembranesarebroadlyrecommendedasanexcellentgeneralpurpose/high-performancesamplefilterformostapplications.

ptfe Polytetrafluoroethylene(PTFE)-Phenex-PTFEmembranesareinherentlyhydrophobicandexcellentforfiltrationof100%organicorhighlyacidicorbasicsamples.

peS Polyethersulfone(PES)-Polyethersulfonemembranesexhibitvery fast-flowandultra-lowproteinbindingcharacteristicsandareideallysuitedforuseinmanylife scienceclarificationapplications.Phenex-PESmembranestypicallyofferbetterchemicalresistancethancelluloseacetateandarebroadlyrecommendedforfilteringcriticalbiologicalsamples, tissueculturemedia,additivesandbuffers.

nY Nylon(NY)-Nylonhasinherenthydrophiliccharacteristicsandworkswellforfiltrationofmany aqueousandmixed-organicsampletypes.Forapplicationsthatrequirelowproteinornon-specificbindingcharacteristics,PhenomenexrecommendsPhenex-rCregeneratedCellulosefilters.

Gf GlassFiber(GF)-Phenex-GFGlassFiberfiltersaremadeofinert borosilicateglassandhaveanominal1.2µmporesize.Theyarecommonlyusedwithhighly viscoussamplesorsamplesthatcontainhighconcentrationsofparticulatematter(e.g.foodanalysis, biologicalsamples,soilsamples,fermentationbrothsamples,removalofyeasts,molds,etc.). CanbeusedaloneorinserieswithotherPhenexfiltermembranessuchasthe0.20µmporePhenex-rCfiltertoreducecloggingofthemembraneandoptimizeflow.

Particle Size

FlowPath

FOOTNOTES:

1. Glass fiber filters are 28 mm diameter and made of borosilicate. They will remove 90 % of all particles >1.2 µm.

2. Housing material is methacrylate butadiene styrene (MBS) polymerisate. Also known as Cyrolite™.

3. Cellulose acetate is surfactant-free.

4. 26 mm diameter.

5. Hydrophobic membrane. Can be made hydrophilic by pre-wetting with IPA.

6. 28 mm diameter.

7. Additional dimensions and membrane types are available. Please contact your local Phenomenex technical consultant or distributor for availability or assistance.

8. Larger quantity purchases at significant savings are available.

Particle Size(µm)

flow Path (µm)

Effective FilterPoreSize(µm)

5 0.72 0.45

3 0.43 0.20

2.6 0.38 0.20

1.7 0.25 0.20

TrademarkedKrudkatcher,Sure-lokandPhenexaretrademarksofPhenomenex,Inc.CyroliteisatrademarkofCY/r0industries.PEEKisatrademarkofICIameica’s.SecurityGuardisatrademarkofPhenomenex,PEEKsilisatrademarkofSGE.KinetexisaregisteredtrademarkofPhenomenexintheU.S.,EU,andotherjurisdictions.

DisclaimersSubjectedtoPhenomenexstandardsTermsandConditionswhichmaybeviewedatwww.phenomenex.com/TermsandConditions

©2011Phenomenex,inc.allrightreserved. GU88

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