control
DESCRIPTION
A. 1 hr. 12 hr. Control. 24 hr. 72 hr. 48 hr. 120 hr. 96 hr. B. C. 100. 20. **. **. No. foci/cell. % foci+ cells. 50. 10. **. **. **. **. 0. 0. **. **. 0. 1. 2. 3. 4. 5. 6. 0. 1. 2. 3. 4. 5. 6. *. *. Days after X-rays. Days after X-rays. - PowerPoint PPT PresentationTRANSCRIPT
Control 12 hr 24 hr
48 hr 72 hr 96 hr 120 hr
1 hr
10
010
Days after X-rays
No.
foc
i/ce
ll
6
20
50
0
% f
oci+
cel
ls
100B C
3 4 5 10Days after X-rays
63 4 5
**
**
**
* *
**
****
****
2 2
A
Figure S1
Induction and repair of DSBs in NSCs receiving 5 Gy of X-rays. A, Immunofluorescent detection of DNA double strand breaks by the foci of phosphorylated histone H2AX at serine 139 (red). Closed arrow heads indicate fragmented nuclei and phosphorylated H2AX-positive intact nuclei, respectively. Bar indicates 5 µm. B, Time-dependent change in the foci number per cell. C, Time-dependent change in the foci-positive cells. The data represent the mean ± SEM. Significance was evaluated between the control and irradiated cells. **p < 0.01, *p < 0.05.