contents introduction - bohemia genetics
TRANSCRIPT
Dongsheng Biotech Co., Ltd. was established in 2005 in
Guangzhou China.
We focus on R&D and produce fundamental molecular
biology reagents. In the past 5 years, Dongsheng Biotech
has successfully grown up from a start-up to a professio-
nal organization. Our products have worldwide sales and
establish an excellent reputation.
We were ISO 9001:2008 certificated by BSI. We are also
authorized import and export manufacture in China. With
professional R&D group and strict control of the production
process, we now have customers all over the world.
Our mission is to provide professional and high quality
products for life science research. To make your research
more professional and more accurate, we are always
making our best efforts.
Introduction
Contents
DNA Laddes & Markers
For technical and sales inquiries:
Dongsheng Biotech Co., Ltd
Tel: +86-20-87791356, +86-20-28983724
Fax: +86-20-87791381
E-mail: [email protected]
Website: www.dongshengbio.com
Ordering Information
5
PCR, qPCR & RT-PCR 20
DNA Purification Kits 38
RNA Purification Kits 46
Silica Spin Column 50
1
2
3
4
Dongsheng Biotech Co., Ltd. was established in 2005 in
Guangzhou China.
We focus on R&D and produce fundamental molecular
biology reagents. In the past 5 years, Dongsheng Biotech
has successfully grown up from a start-up to a professio-
nal organization. Our products have worldwide sales and
establish an excellent reputation.
We were ISO 9001:2008 certificated by BSI. We are also
authorized import and export manufacture in China. With
professional R&D group and strict control of the production
process, we now have customers all over the world.
Our mission is to provide professional and high quality
products for life science research. To make your research
more professional and more accurate, we are always
making our best efforts.
Introduction
Contents
DNA Laddes & Markers
For technical and sales inquiries:
Dongsheng Biotech Co., Ltd
Tel: +86-20-87791356, +86-20-28983724
Fax: +86-20-87791381
E-mail: [email protected]
Website: www.dongshengbio.com
Ordering Information
5
PCR, qPCR & RT-PCR 20
DNA Purification Kits 38
RNA Purification Kits 46
Silica Spin Column 50
1
2
3
4
Key Products :
•
•
•
•
•
Taq DNA Polymerases
PCR Mix
DNA Ladders
PCR & Gel Extraction Kits
TRAzol
Product Line:
1. DNA Electrophoresis Products
2. PCR Products
3. DNA Purification Products
4, RNA Purification Products
5. Other Associative Reagents
.
Dongsheng Biotech provides wide range of molecular
biology products for life science research.
Additionally, all product provides custom synthesis.
Make Your Research
More Professional
More Accurate (1) Laboratory reagent kits producers, a smaller group of custo-
mers (<50 major customers worldwide) who purchase substa-
ntial quantities of nucleotide monomers as raw material for
specialized laboratory kits for use in research laboratories.
(2) Diagnostic kits producers who use our nucleotide monomers
as diagnostic agents in hospitals, blood collection agencies
and pathology laboratories primarily for amplification.
(3) Research laboratories in universities, industries, governments
and hospitals & medical schools.
Serve three major customer groups:
Dongsheng Biotech Product Application area
Key Products :
•
•
•
•
•
Taq DNA Polymerases
PCR Mix
DNA Ladders
PCR & Gel Extraction Kits
TRAzol
Product Line:
1. DNA Electrophoresis Products
2. PCR Products
3. DNA Purification Products
4, RNA Purification Products
5. Other Associative Reagents
.
Dongsheng Biotech provides wide range of molecular
biology products for life science research.
Additionally, all product provides custom synthesis.
Make Your Research
More Professional
More Accurate (1) Laboratory reagent kits producers, a smaller group of custo-
mers (<50 major customers worldwide) who purchase substa-
ntial quantities of nucleotide monomers as raw material for
specialized laboratory kits for use in research laboratories.
(2) Diagnostic kits producers who use our nucleotide monomers
as diagnostic agents in hospitals, blood collection agencies
and pathology laboratories primarily for amplification.
(3) Research laboratories in universities, industries, governments
and hospitals & medical schools.
Serve three major customer groups:
Dongsheng Biotech Product Application area
10bp ladder
20bp ladder
low ladder
50bp ladder
50bp ladder plus
100bp ladder
100 bp ladder plus
1kb ladder
1kb ladder plus
200 bp ladder
500bp ladder
?DNA/Hind
Marker 1
Marker 2
Marker 3
Marker 4
TMDS 2000
TMDS 5000
TMDS 10000
TMDS 15000
M1011
M1012
M1021
M1022
M1031
M1032
M1041
M1042
M1051
M1052
M1061
M1062
M1071
M1072
M1181
M1182
M1191
M1192
M1151
M1152
M1251
M1252
M1201
M1202
M1081
M1082
M1091
M1092
M1121
M1122
M1231
M1232
M1101
M1102
M1111
M1112
M1221
M1222
M1161
M1162
80-300bp
60-300bp
25-700bp
50-500bp
50-1,000bp
100-1,500bp
100-3,000bp
500-10,000bp
100-10,000bp
200-4,000bp
500-5,000bp
125-23,130bp
100-600bp
100-1,200bp
200-4,500bp
500-15,000bp
100-2,000bp
100-5,000bp
250-10,000bp
250-15,000bp
Product name Cat # Size Range
20x Swift DNA Electrophoresis Buffe
50xTAE Buffer
10xTBE
Gel Loading Dye(6x)
Gel Loading Dye, Blue(6x)
Gel Loading Dye, Three-colour (6x)
Gel Loading Dye, Orange(6x)
Gel Loading Dye, SDS (6x)
DSView Nucleic Acid Stain
Agarose
TM
M9011
M9021
M9031
M9041
M9051
M9061
M9071
M9081
M7011
N9051
500ml
500ml
500ml
5x1ml
5x1ml
5x1ml
5x1ml
5x1ml
1ml
500g
DNA Ladders & Markers Product List
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1. DNA Ladders & Markers
E-mail: [email protected] Fax: +86-20-87791381 6
10bp ladder
20bp ladder
low ladder
50bp ladder
50bp ladder plus
100bp ladder
100 bp ladder plus
1kb ladder
1kb ladder plus
200 bp ladder
500bp ladder
?DNA/Hind
Marker 1
Marker 2
Marker 3
Marker 4
TMDS 2000
TMDS 5000
TMDS 10000
TMDS 15000
M1011
M1012
M1021
M1022
M1031
M1032
M1041
M1042
M1051
M1052
M1061
M1062
M1071
M1072
M1181
M1182
M1191
M1192
M1151
M1152
M1251
M1252
M1201
M1202
M1081
M1082
M1091
M1092
M1121
M1122
M1231
M1232
M1101
M1102
M1111
M1112
M1221
M1222
M1161
M1162
80-300bp
60-300bp
25-700bp
50-500bp
50-1,000bp
100-1,500bp
100-3,000bp
500-10,000bp
100-10,000bp
200-4,000bp
500-5,000bp
125-23,130bp
100-600bp
100-1,200bp
200-4,500bp
500-15,000bp
100-2,000bp
100-5,000bp
250-10,000bp
250-15,000bp
Product name Cat # Size Range
20x Swift DNA Electrophoresis Buffe
50xTAE Buffer
10xTBE
Gel Loading Dye(6x)
Gel Loading Dye, Blue(6x)
Gel Loading Dye, Three-colour (6x)
Gel Loading Dye, Orange(6x)
Gel Loading Dye, SDS (6x)
DSView Nucleic Acid Stain
Agarose
TM
M9011
M9021
M9031
M9041
M9051
M9061
M9071
M9081
M7011
N9051
500ml
500ml
500ml
5x1ml
5x1ml
5x1ml
5x1ml
5x1ml
1ml
500g
DNA Ladders & Markers Product List
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http://dongshengbio.com Tel: +86-20-877913565
1. DNA Ladders & Markers
E-mail: [email protected] Fax: +86-20-87791381 6
PCR and plasmid digestion by restriction enzymes are used for
the production of DNA fragments for our ladders/markers.
These products are stable during prolonged incubations at
Distinguishing features:
DNA Markers and Ladders
room temperature and multiple freeze-thaw cycles.
Storage
Bright, sharp bands for highly accurate sizing
Precise DNA concentration, quantitative
High resolution, low-background
Stable and uniform with different batches
Stable for 3 months at RT, 2 years -20℃.
Guideline for DNA Electrophoresis
Table 1. Different kinds of Agarose for different size of DNA range.
Agarose (%) Standard agarose High EEO agarose Low melt agarose Low melt low EEO agarose
Table 2. Different concentration of SDS-PAGE gel for different size of DNA range
SDS-PAGE gel (Polyacrylamide gel) is for separation of small size DNA range (≤1000bp)
SDS-PAGE gel (%)
3.55.
Effective DNA range
100 bp~1 kb80 bp~5
1. Electrophoresis gel
1.1 Agarose gel
1.2 SDS-PAGE gel
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2. Gel electrophoresis system
2.1 Electrophoresis buffer
Table 3.Guideline for electrophoresis buffer
Type
Swift DNAelecctrophoresis buffer
Feature
High speed
Low buffer capabilityHigh speed for ds linear DNATAE Buffer
TBE Buffer High buffer capabilityGood DNA separation, but low recovery rate
Application
High voltage and quick electrophoresisSmall amount but needs recovery DNA
Recommend for genomic DNAMacromolecular super helical DNADNA or RNA above 1500 bp
Recommend for DNA or RNA below 1500 bp
2.2 Electrophoresis condition
Table 4. Guideline for voltage and buffers for agarose gel electrophoresis
DNA size
<1 kb
1~12 kb
>12 kb
High throughput electrophoresis (~10kb)
Voltage
5~10 V/cm
4~10V/cm
1~3 V/cm
~23 V/cm
Buffer
TEB or Swift DNA electrophoresis buffer
TAE or TBE or Swift DNA electrophoresis buffer
TAE or Swift DNA electrophoresis buffer
Swift DNA electrophoresis buffer
DNA mobility in electrophoresis is effective by DNA size and structure, electrophoresis voltage, etc.
3. Guideline for DNA Ladders choice
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E-mail: [email protected] Fax: +86-20-87791381 8
DNA Ladders & Markers
PCR and plasmid digestion by restriction enzymes are used for
the production of DNA fragments for our ladders/markers.
These products are stable during prolonged incubations at
Distinguishing features:
DNA Markers and Ladders
room temperature and multiple freeze-thaw cycles.
Storage
Bright, sharp bands for highly accurate sizing
Precise DNA concentration, quantitative
High resolution, low-background
Stable and uniform with different batches
Stable for 3 months at RT, 2 years -20℃.
Guideline for DNA Electrophoresis
Table 1. Different kinds of Agarose for different size of DNA range.
Agarose (%) Standard agarose High EEO agarose Low melt agarose Low melt low EEO agarose
Table 2. Different concentration of SDS-PAGE gel for different size of DNA range
SDS-PAGE gel (Polyacrylamide gel) is for separation of small size DNA range (≤1000bp)
SDS-PAGE gel (%)
3.55.
Effective DNA range
100 bp~1 kb80 bp~5
1. Electrophoresis gel
1.1 Agarose gel
1.2 SDS-PAGE gel
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2. Gel electrophoresis system
2.1 Electrophoresis buffer
Table 3.Guideline for electrophoresis buffer
Type
Swift DNAelecctrophoresis buffer
Feature
High speed
Low buffer capabilityHigh speed for ds linear DNATAE Buffer
TBE Buffer High buffer capabilityGood DNA separation, but low recovery rate
Application
High voltage and quick electrophoresisSmall amount but needs recovery DNA
Recommend for genomic DNAMacromolecular super helical DNADNA or RNA above 1500 bp
Recommend for DNA or RNA below 1500 bp
2.2 Electrophoresis condition
Table 4. Guideline for voltage and buffers for agarose gel electrophoresis
DNA size
<1 kb
1~12 kb
>12 kb
High throughput electrophoresis (~10kb)
Voltage
5~10 V/cm
4~10V/cm
1~3 V/cm
~23 V/cm
Buffer
TEB or Swift DNA electrophoresis buffer
TAE or TBE or Swift DNA electrophoresis buffer
TAE or Swift DNA electrophoresis buffer
Swift DNA electrophoresis buffer
DNA mobility in electrophoresis is effective by DNA size and structure, electrophoresis voltage, etc.
3. Guideline for DNA Ladders choice
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DNA Ladders & Markers
5 µl/lane, 8 cm gel0.5×TBE, 5 V/cm, 1h
─ 300 ? 280? 260
? 240? 220
? 200
? 180
? 160
? 140
? 120
? 100
? 60
? 80
40
40
40
100
40
40
40
40
100
40
40
40
40
10
% S
DS
-PA
GE
bp ng/5l
10bp ladder M1011/M1012
─ 300
bp ng/5l
? 200? 190
? 160
? 140
? 150
? 130
? 120
? 110
? 100
? 90
? 80
4040
100
40
40
100
40
40
40
40
40
40
─ 280 ─ 260 ─ 240
─ 220
? 180? 170
4040
40
404040
10
% S
DS
-PA
GE
10bp ladder is ideal for determining the size of double-stranded DNA from 80 to
300bp base pairs. The ladder consists of 18 linear double-stranded fragments.
The and p fragment are present at increased intensity to allow easy
identification. All fragments are precisely quantified and mixed during the
production. For 5 l loading, all fragments except 100bp and 200bp are 40ng. The
100bp and 200bp fragment are 100ng. This ladder is pre-mixed with loading dye
100bp 200b
Description
and is ready to use.
Contents(bp)
80, 90, , 110, 120, 130, 140, ,
220, 240,260, 280, 300
100 150, 160,170,180, 190, 200
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 168ng/ l
Recommended Electrophoresis Condition
8cm 5% Agarose Gel, 0.5x TBE, 5V/cm, 1h
20cm 10% SDS-PAGE Gel, 1x TBE, 8V/cm, 3h
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
10 bp ladder Cat: M1011-50µg, M1012-5x50µg
20 bp ladder Cat. # M1021-50µg, M1022-5x50µg
20bp ladder is ideal for determining the size of double-stranded DNA from 60 to
300bp base pairs. The ladder consists of 13 linear double-stranded fragments.
The and p fragment are present at increased intensity to allow easy
identification. All fragments are precisely quantified and mixed during the
production. For 5 l loading, all fragments except 100bp and 200bp are 40ng. The
100bp and 200bp fragment are 100ng. This ladder is pre-mixed with loading dye
100bp 200b
Description
and is ready to use.
Contents(bp)
60, 80, , 120, 140, 160, 180, , 220, 240,260, 280, 300100 200
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 128ng/ l
Recommended Electrophoresis Condition
8cm 5% Agarose Gel, 0.5x TBE, 5V/cm, 1h
20cm 10% SDS-PAGE Gel, 1x TBE, 8V/cm, 3h
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
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10
3%
ag
aro
se
3%
ag
aro
se
50bp ladder is ideal for determining the size of double-stranded DNA from 50 to
500bp base pairs. The ladder consists of 8 linear double-stranded fragments.
The fragment is present at increased intensity to allow easy identification.
All fragments are precisely quantified and mixed during the production. For
5 l loading, all fragments except 250bp is 40ng. The 250bp is 100ng. This ladder
250bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
50, 100, 200, , 300, 400,500150, 250
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 76ng/ l
Recommended Electrophoresis Condition
8cm 3% Agarose Gel, 0.5x TBE, 5V/cm, 1h
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
50 bp ladder Cat: M1041-50µg, M1042-5x50µg
50 bp ladder plus Cat. # M1051-50µg, M1052-5x50µg
50bp ladder plus is ideal for determining the size of double-stranded DNA from 50 to
1000bp base pairs. The ladder consists of 13 linear double-stranded fragments.
The and p fragment are present at increased intensity to allow easy
identification. All fragments are precisely quantified and mixed during the
production. For 5 l loading, all fragments except 250bp and 500bp are 40ng. The
250bp and 500bp fragment are 100ng. This ladder is pre-mixed wi th loading dye
250bp 500b
Description
and is ready to use.
Contents(bp)
50, 100, ,300, 400, , 600, 700,800, 900, 1000 150, 200, 250 500
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 128ng/ l
Recommended Electrophoresis Condition
8cm 3% Agarose Gel, 0.5x TBE, 5V/cm, 1h
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
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E-mail: [email protected] Fax: +86-20-87791381
DNA Ladders & Markers
5 µl/lane, 8 cm gel0.5×TBE, 5 V/cm, 1h
─ 300 ? 280? 260
? 240? 220
? 200
? 180
? 160
? 140
? 120
? 100
? 60
? 80
40
40
40
100
40
40
40
40
100
40
40
40
40
10
% S
DS
-PA
GE
bp ng/5l
10bp ladder M1011/M1012
─ 300
bp ng/5l
? 200? 190
? 160
? 140
? 150
? 130
? 120
? 110
? 100
? 90
? 80
4040
100
40
40
100
40
40
40
40
40
40
─ 280 ─ 260 ─ 240
─ 220
? 180? 170
4040
40
404040
10
% S
DS
-PA
GE
10bp ladder is ideal for determining the size of double-stranded DNA from 80 to
300bp base pairs. The ladder consists of 18 linear double-stranded fragments.
The and p fragment are present at increased intensity to allow easy
identification. All fragments are precisely quantified and mixed during the
production. For 5 l loading, all fragments except 100bp and 200bp are 40ng. The
100bp and 200bp fragment are 100ng. This ladder is pre-mixed with loading dye
100bp 200b
Description
and is ready to use.
Contents(bp)
80, 90, , 110, 120, 130, 140, ,
220, 240,260, 280, 300
100 150, 160,170,180, 190, 200
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 168ng/ l
Recommended Electrophoresis Condition
8cm 5% Agarose Gel, 0.5x TBE, 5V/cm, 1h
20cm 10% SDS-PAGE Gel, 1x TBE, 8V/cm, 3h
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
10 bp ladder Cat: M1011-50µg, M1012-5x50µg
20 bp ladder Cat. # M1021-50µg, M1022-5x50µg
20bp ladder is ideal for determining the size of double-stranded DNA from 60 to
300bp base pairs. The ladder consists of 13 linear double-stranded fragments.
The and p fragment are present at increased intensity to allow easy
identification. All fragments are precisely quantified and mixed during the
production. For 5 l loading, all fragments except 100bp and 200bp are 40ng. The
100bp and 200bp fragment are 100ng. This ladder is pre-mixed with loading dye
100bp 200b
Description
and is ready to use.
Contents(bp)
60, 80, , 120, 140, 160, 180, , 220, 240,260, 280, 300100 200
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 128ng/ l
Recommended Electrophoresis Condition
8cm 5% Agarose Gel, 0.5x TBE, 5V/cm, 1h
20cm 10% SDS-PAGE Gel, 1x TBE, 8V/cm, 3h
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
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10
3%
ag
aro
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3%
ag
aro
se
50bp ladder is ideal for determining the size of double-stranded DNA from 50 to
500bp base pairs. The ladder consists of 8 linear double-stranded fragments.
The fragment is present at increased intensity to allow easy identification.
All fragments are precisely quantified and mixed during the production. For
5 l loading, all fragments except 250bp is 40ng. The 250bp is 100ng. This ladder
250bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
50, 100, 200, , 300, 400,500150, 250
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 76ng/ l
Recommended Electrophoresis Condition
8cm 3% Agarose Gel, 0.5x TBE, 5V/cm, 1h
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
50 bp ladder Cat: M1041-50µg, M1042-5x50µg
50 bp ladder plus Cat. # M1051-50µg, M1052-5x50µg
50bp ladder plus is ideal for determining the size of double-stranded DNA from 50 to
1000bp base pairs. The ladder consists of 13 linear double-stranded fragments.
The and p fragment are present at increased intensity to allow easy
identification. All fragments are precisely quantified and mixed during the
production. For 5 l loading, all fragments except 250bp and 500bp are 40ng. The
250bp and 500bp fragment are 100ng. This ladder is pre-mixed wi th loading dye
250bp 500b
Description
and is ready to use.
Contents(bp)
50, 100, ,300, 400, , 600, 700,800, 900, 1000 150, 200, 250 500
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 128ng/ l
Recommended Electrophoresis Condition
8cm 3% Agarose Gel, 0.5x TBE, 5V/cm, 1h
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
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E-mail: [email protected] Fax: +86-20-87791381
DNA Ladders & Markers
100bp ladder is ideal for determining the size of double-stranded DNA from 100 to
1500bp base pairs. The ladder consists of 11 linear double-stranded fragments.
The fragment is present at increased intensity to allow easy identification.
All fragments are precisely quantified and mixed during the production. For
5 l loading, all fragments except 500bp is 40ng. The 500bp is 100ng. This ladder
500bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
100, 300,400, , 600,700,800,900,1000,1500200, 500
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 100ng/ l
Recommended Electrophoresis Condition
8cm 1.7% Agarose Gel, 0.5x TBE, 5V/cm, 1h
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
100 bp ladder Cat: M1061-50µg, M1062-5x50µg
100bp ladder plus is ideal for determining the size of double-st randed DNA from 100 to
3000bp base pairs. The ladder consists of 14 linear double-st randed fragments.
The and fragment are present at increa sed intensi ty to allow easy
identif ication. Al l fragments are precisely quantif ied and mixed during the
product ion. For 5 l loading, all fragments except 500bp and 1200bp are 40ng. The
500bp and 1200bp fragment are 100ng. This ladder is pre-mixed with loading dye
500bp 1200bp
Description
and is ready to use.
Contents(bp)
100, 200, ,600,700,800,900,1000, , 1500,2000,3000300, 400,500 1200
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 136ng/ l
Recommended Electrophoresis Condition
8cm 1.7% Agarose Gel, 0.5x TBE, 5V/cm, 1h
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
100 bp ladder plus Cat. # M1071-50µg, M1072-5x50µg
1.7
% a
ga
rose
1.7
% a
ga
rose
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1kb ladder is ideal for determining the size of double-stranded DNA from 500 to
10000bp base pairs. The ladder consists of 10 linear double-stranded fragments.
The and fragments are present at increased intensity to allow easy
identification. Al l fragments are precisely quantified and mixed during the production.
For 5 l loading, all fragments except 2000bp and 5000bp are 40ng. The 2000bp
and 5000bp are 100ng. Th is ladder
2000bp 5000bp
is pre-mixed wi th loading dye and is ready to use.
Description
Contents(bp)
500, 1000, , 3000, 4000, 1500, , 6000, 8000, 100002000 5000
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 104ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
1 kb ladder Cat: M1181-50µg, M1182-5x50µg
1kb ladder plus is ideal for determining the size of double-stranded DNA from 100 to
10000bp base pa irs. The ladder consists of 15 linear double-stranded fragments.
The and p fragment are present at increased intensity to al low easy
ident ificat ion . Al l fragments are prec isely quantified and mixed during the
production. For 5 l loading, al l fragments except 500bp and 3000bp are 40ng. The
500bp and 3000bp fragment are 100ng. Thi s ladder is pre-mixed wi th loading dye
500bp 3000b
Description
and is ready to use.
Contents(bp)
100, ,700,1000,1500,2000, , 4000, 5000,6000, 8000, 10000 200, 300,400, 500 3000
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 144ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min
1kb ladder plus Cat. # M1191-50µg, M1192-5x50µg
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l1
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E-mail: [email protected] Fax: +86-20-87791381 12
DNA Ladders & Markers
100bp ladder is ideal for determining the size of double-stranded DNA from 100 to
1500bp base pairs. The ladder consists of 11 linear double-stranded fragments.
The fragment is present at increased intensity to allow easy identification.
All fragments are precisely quantified and mixed during the production. For
5 l loading, all fragments except 500bp is 40ng. The 500bp is 100ng. This ladder
500bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
100, 300,400, , 600,700,800,900,1000,1500200, 500
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 100ng/ l
Recommended Electrophoresis Condition
8cm 1.7% Agarose Gel, 0.5x TBE, 5V/cm, 1h
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
100 bp ladder Cat: M1061-50µg, M1062-5x50µg
100bp ladder plus is ideal for determining the size of double-st randed DNA from 100 to
3000bp base pairs. The ladder consists of 14 linear double-st randed fragments.
The and fragment are present at increa sed intensi ty to allow easy
identif ication. Al l fragments are precisely quantif ied and mixed during the
product ion. For 5 l loading, all fragments except 500bp and 1200bp are 40ng. The
500bp and 1200bp fragment are 100ng. This ladder is pre-mixed with loading dye
500bp 1200bp
Description
and is ready to use.
Contents(bp)
100, 200, ,600,700,800,900,1000, , 1500,2000,3000300, 400,500 1200
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 136ng/ l
Recommended Electrophoresis Condition
8cm 1.7% Agarose Gel, 0.5x TBE, 5V/cm, 1h
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
100 bp ladder plus Cat. # M1071-50µg, M1072-5x50µg
1.7
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1kb ladder is ideal for determining the size of double-stranded DNA from 500 to
10000bp base pairs. The ladder consists of 10 linear double-stranded fragments.
The and fragments are present at increased intensity to allow easy
identification. Al l fragments are precisely quantified and mixed during the production.
For 5 l loading, all fragments except 2000bp and 5000bp are 40ng. The 2000bp
and 5000bp are 100ng. Th is ladder
2000bp 5000bp
is pre-mixed wi th loading dye and is ready to use.
Description
Contents(bp)
500, 1000, , 3000, 4000, 1500, , 6000, 8000, 100002000 5000
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 104ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
1 kb ladder Cat: M1181-50µg, M1182-5x50µg
1kb ladder plus is ideal for determining the size of double-stranded DNA from 100 to
10000bp base pa irs. The ladder consists of 15 linear double-stranded fragments.
The and p fragment are present at increased intensity to al low easy
ident ificat ion . Al l fragments are prec isely quantified and mixed during the
production. For 5 l loading, al l fragments except 500bp and 3000bp are 40ng. The
500bp and 3000bp fragment are 100ng. Thi s ladder is pre-mixed wi th loading dye
500bp 3000b
Description
and is ready to use.
Contents(bp)
100, ,700,1000,1500,2000, , 4000, 5000,6000, 8000, 10000 200, 300,400, 500 3000
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 144ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min
1kb ladder plus Cat. # M1191-50µg, M1192-5x50µg
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
1%
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DNA Ladders & Markers
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Low ladder is ideal for det ermining the size of doubl e-st randed DNA from 25bp to
700bp base pai rs. The ladder cons ists of 10 linear doubl e-st randed fragm ent s.
The and fragm ent s are presen t at incr ease d intensi ty to al low easy
ident ificat ion. Al l fragm ent s are preci sel y quant ified and mixed dur ing the produc tion.
For 5 l loadi ng, al l fragm ent s exc ept 100bp and 300bp are 40ng. The 100bp and
100bp 300bp
Description
Contents(bp)
25, , , 150,200, ,400,500,70050,75 100 300
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 104ng/ l
Recommended Electrophoresis Condition
8cm 3% Agarose Gel, 0.5x TBE, 5V/cm, 1h
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
Low ladder Cat: M1031-50µg, M1032-5x50µg
200bp ladder is ideal for determining the size of double-stranded DNA from 200 to
4000bp base pa irs. The ladder consists of 12 linear double-stranded fragments. The
fragment is present at increased intensity to al low easy ident ificat ion. Al l
fragments are prec isely quantified and mixed during the production. For 5 l loading,
al l fragments except 1000bp is 40ng. The 1000bp fragment is 100ng. Thi s ladder is
1000bp
Description
pre-mixed with loading dye and is ready to use.
Contents(bp)
200, ,1200,1400,1600,1800,2000,2200,4000400, 600,800,1000
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 108ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 40min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
200bp ladder Cat. # M1151-50µg, M1152-5x50µg
300bp are 100ng. This ladder is pre-mixed with loading dye and is ready to use.
1%
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500bp ladder is ideal for determining the size of double-stranded DNA from 500 to
5000bp base pairs. The ladder consists of 10 linear double-stranded fragments.
The fragment is present at increased intensity to allow easy identification.
All fragments are precisely quantified and mixed during the production. For 5 l
loading, all fragments except 2000bp are 40ng. The 2000bp is 100ng. This ladder
2000bp
Description
Contents(bp)
500,1000, , 2500, 3000,3500,4000, 1500, 4500, 5000, 2000
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 92ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
500bp ladder Cat: M1251-50µg, M1252-5x50µg
The Hind III digest of lambda DNA yields 8 fragments suitable for use as
molecular weight standards for agarose gel electrophoresis. Lambda
DNA/Hind III is pre-mixed with loading dye and is ready to use.
Description
Contents(bp)
125, 564, 2027,2322,4361,6557,9461,23130
Storage:
3 months at room temperature, 2 years at -20℃.
Recommended Electrophoresis Condition
8cm 0.7% Agarose Gel, 1x TAE, 7V/cm, 45min
Lambda DNA/Hind III Cat. # M1201-50µg, M1202-5x50µg
Recommended Loading : 2-5 l/lane
is pre-mixed with loading dye and is ready to use.
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E-mail: [email protected] Fax: +86-20-87791381 14
DNA Ladders & Markers
1%
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3%
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Low ladder is ideal for det ermining the size of doubl e-st randed DNA from 25bp to
700bp base pai rs. The ladder cons ists of 10 linear doubl e-st randed fragm ent s.
The and fragm ent s are presen t at incr ease d intensi ty to al low easy
ident ificat ion. Al l fragm ent s are preci sel y quant ified and mixed dur ing the produc tion.
For 5 l loadi ng, al l fragm ent s exc ept 100bp and 300bp are 40ng. The 100bp and
100bp 300bp
Description
Contents(bp)
25, , , 150,200, ,400,500,70050,75 100 300
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 104ng/ l
Recommended Electrophoresis Condition
8cm 3% Agarose Gel, 0.5x TBE, 5V/cm, 1h
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
Low ladder Cat: M1031-50µg, M1032-5x50µg
200bp ladder is ideal for determining the size of double-stranded DNA from 200 to
4000bp base pa irs. The ladder consists of 12 linear double-stranded fragments. The
fragment is present at increased intensity to al low easy ident ificat ion. Al l
fragments are prec isely quantified and mixed during the production. For 5 l loading,
al l fragments except 1000bp is 40ng. The 1000bp fragment is 100ng. Thi s ladder is
1000bp
Description
pre-mixed with loading dye and is ready to use.
Contents(bp)
200, ,1200,1400,1600,1800,2000,2200,4000400, 600,800,1000
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 108ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 40min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
200bp ladder Cat. # M1151-50µg, M1152-5x50µg
300bp are 100ng. This ladder is pre-mixed with loading dye and is ready to use.
1%
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http://dongshengbio.com Tel: +86-20-8779135613
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500bp ladder is ideal for determining the size of double-stranded DNA from 500 to
5000bp base pairs. The ladder consists of 10 linear double-stranded fragments.
The fragment is present at increased intensity to allow easy identification.
All fragments are precisely quantified and mixed during the production. For 5 l
loading, all fragments except 2000bp are 40ng. The 2000bp is 100ng. This ladder
2000bp
Description
Contents(bp)
500,1000, , 2500, 3000,3500,4000, 1500, 4500, 5000, 2000
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 92ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 100ng/ l, Other bands: 40ng/ l
500bp ladder Cat: M1251-50µg, M1252-5x50µg
The Hind III digest of lambda DNA yields 8 fragments suitable for use as
molecular weight standards for agarose gel electrophoresis. Lambda
DNA/Hind III is pre-mixed with loading dye and is ready to use.
Description
Contents(bp)
125, 564, 2027,2322,4361,6557,9461,23130
Storage:
3 months at room temperature, 2 years at -20℃.
Recommended Electrophoresis Condition
8cm 0.7% Agarose Gel, 1x TAE, 7V/cm, 45min
Lambda DNA/Hind III Cat. # M1201-50µg, M1202-5x50µg
Recommended Loading : 2-5 l/lane
is pre-mixed with loading dye and is ready to use.
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E-mail: [email protected] Fax: +86-20-87791381 14
DNA Ladders & Markers
3%
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Mar ker 1 is ideal for determining the size of double-stranded DNA from 100 to
600bp base pairs. The ladder consists of 6 linear double-stranded fragmen ts.
The fragmen t is present at increased intensity to allow easy identification.
Al l fragmen ts are precisely quantified and mixed during the production. For
5 l loading, all fragmen ts except 400bp are 50ng. The 400bp is 125ng. Th is ladder
400bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
100, 300, , 500, 600200, 400
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 76ng/ l
Recommended Electrophoresis Condition
8cm 3% Agarose Gel, 0.5x TBE, 7V/cm, 40min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 125ng/ l, Other bands: 50ng/ l
Marker 1 Cat: M1081-50µg, M1082-5x50µg
Marker 2 is ideal for determining the size of double-stranded DNA from 100 to
1200bp base pa irs. The ladder consists of 6 linear double-stranded fragments.
The fragment is present at increased intensity to al low easy ident ificat ion.
Al l fragments are prec isely quantified and mixed during the production. For
5 l loading, al l fragments except 700bp are 50ng. The 700bp is 125ng. Thi s ladder
700bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
100, , 900,1200300, 500, 600, 700
Concentration: 75ng/ l
Recommended Electrophoresis Condition
8cm 1.7% Agarose Gel, 0.5x TBE, 5V/cm, 1h
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 125ng/ l, Other bands: 50ng/ l
Marker 2 Cat. # M1091-50µg, M1092-5x50µg
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Storage:
3 months at room temperature, 2 years at -20℃.
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Marker 3 is ideal for determining the size of double-stranded DNA from 200 to
4500bp base pa irs. The ladder consists of 7 linear double-stranded fragments.
The fragment is present at increased intensity to al low easy ident ificat ion.
Al l fragments are prec isely quantified and mixed during the production. For
5 l loading, al l fragments except 1200bp are 75ng. The 1200bp is 150ng. Th is ladder
1200bp
Description
Contents(bp)
200, 500, , 2000, 3000,800, 45001200
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 85ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 40min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 150ng/ l, Other bands: 75ng/ l
Marker 3 Cat: M1121-50µg, M1122-5x50µg
Marker 4 is ideal for det ermining the size of doubl e-st randed DNA from 500 to
15000bp base pai rs. The ladder cons ists of 6 linear doubl e-st randed fragm ent s.
The fragm ent is present at incr eased intensi ty to al low easy ident ificat ion.
Al l fragm ent s are preci sel y quant ified and mixed dur ing the product ion. For
5 l loadi ng, al l fragm ent s exc ept 2500bp are 50ng. The 2500bp is 125ng. Thi s ladder
2500bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
500, , 5000, 8000,15000 1200, 2500
Concentration: 75ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min
Marker 4 Cat. # M1231-50µg, M1232-5x50µg
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 125ng/ l, Other bands: 50ng/ l1
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is pre-mixed with loading dye and is ready to use.
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E-mail: [email protected] Fax: +86-20-87791381 16
DNA Ladders & Markers
Storage:
3 months at room temperature, 2 years at -20℃.
3%
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Mar ker 1 is ideal for determining the size of double-stranded DNA from 100 to
600bp base pairs. The ladder consists of 6 linear double-stranded fragmen ts.
The fragmen t is present at increased intensity to allow easy identification.
Al l fragmen ts are precisely quantified and mixed during the production. For
5 l loading, all fragmen ts except 400bp are 50ng. The 400bp is 125ng. Th is ladder
400bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
100, 300, , 500, 600200, 400
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 76ng/ l
Recommended Electrophoresis Condition
8cm 3% Agarose Gel, 0.5x TBE, 7V/cm, 40min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 125ng/ l, Other bands: 50ng/ l
Marker 1 Cat: M1081-50µg, M1082-5x50µg
Marker 2 is ideal for determining the size of double-stranded DNA from 100 to
1200bp base pa irs. The ladder consists of 6 linear double-stranded fragments.
The fragment is present at increased intensity to al low easy ident ificat ion.
Al l fragments are prec isely quantified and mixed during the production. For
5 l loading, al l fragments except 700bp are 50ng. The 700bp is 125ng. Thi s ladder
700bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
100, , 900,1200300, 500, 600, 700
Concentration: 75ng/ l
Recommended Electrophoresis Condition
8cm 1.7% Agarose Gel, 0.5x TBE, 5V/cm, 1h
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 125ng/ l, Other bands: 50ng/ l
Marker 2 Cat. # M1091-50µg, M1092-5x50µg
1.7
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http://dongshengbio.com Tel: +86-20-8779135615
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Storage:
3 months at room temperature, 2 years at -20℃.
1%
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Marker 3 is ideal for determining the size of double-stranded DNA from 200 to
4500bp base pa irs. The ladder consists of 7 linear double-stranded fragments.
The fragment is present at increased intensity to al low easy ident ificat ion.
Al l fragments are prec isely quantified and mixed during the production. For
5 l loading, al l fragments except 1200bp are 75ng. The 1200bp is 150ng. Th is ladder
1200bp
Description
Contents(bp)
200, 500, , 2000, 3000,800, 45001200
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 85ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 40min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 150ng/ l, Other bands: 75ng/ l
Marker 3 Cat: M1121-50µg, M1122-5x50µg
Marker 4 is ideal for det ermining the size of doubl e-st randed DNA from 500 to
15000bp base pai rs. The ladder cons ists of 6 linear doubl e-st randed fragm ent s.
The fragm ent is present at incr eased intensi ty to al low easy ident ificat ion.
Al l fragm ent s are preci sel y quant ified and mixed dur ing the product ion. For
5 l loadi ng, al l fragm ent s exc ept 2500bp are 50ng. The 2500bp is 125ng. Thi s ladder
2500bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
500, , 5000, 8000,15000 1200, 2500
Concentration: 75ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min
Marker 4 Cat. # M1231-50µg, M1232-5x50µg
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 125ng/ l, Other bands: 50ng/ l
1%
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is pre-mixed with loading dye and is ready to use.
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E-mail: [email protected] Fax: +86-20-87791381 16
DNA Ladders & Markers
Storage:
3 months at room temperature, 2 years at -20℃.
DS 2000 is ideal for determining the size of double-stranded DNA from 100 to
2000bp base pairs. The ladder consists of 6 linear double-stranded fragmen ts.
The fragmen t is present at increased intensity to allow easy identification.
Al l fragmen ts are precisely quantified and mixed during the production. For
5 l loading, all fragmen ts except 750bp are 75ng. The 750bp is 150ng. Th is ladder
750bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
100, 500, , 1000, 2000200, 750
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 105ng/ l
Recommended Electrophoresis Condition
8cm 1.7% Agarose Gel, 0.5x TBE, 7V/cm, 40min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 150ng/ l, Other bands: 75ng/ l
DS 2000 Cat: M1101-50µg, M1102-5x50µg1
.7%
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TM
TM
DS 5000 is ideal for determining the size of double-stranded DNA from 100 to
5000bp base pa irs. The ladder consists of 9 linear double-stranded fragments.
The fragment is present at increased intensity to al low easy ident ificat ion.
Al l fragments are prec isely quantified and mixed during the production. For
5 l loading, al l fragments except 1000bp are 75ng. The 1500bp is 150ng. Thi s ladder
1000bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
100, 250, 500, 750, , 1500, 2000, 3000, 50001000
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 150ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 40min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 150ng/ l, Other bands: 75ng/ l
DS 5000 Cat: M1111-50µg, M1112-5x50µgTM
TM
1%
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http://dongshengbio.com Tel: +86-20-8779135617
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1%
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1%
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DS 10000 is ideal for det ermining the size of doubl e-st randed DNA from 250 to
10000bp base pai rs. The ladder cons ists of 7 linear doubl e-st randed fragm ent s.
The fragm ent is presen t at incr ease d intensi ty to al low easy ident ificat ion.
Al l fragm ent s are preci sel y quant ified and mixed dur ing the produc tion. For
5 l loadi ng, al l fragm ent s exc ept 2000bp are 50ng. The 2000bp is 125ng. Thi s ladder
2000bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
250, 1000, , 4000,7000, 10000500, 2000
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 85ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 125ng/ l, Other bands: 50ng/ l
DS 10000 Cat: M1221-50µg, M1222-5x50µgTM
TM
DS 15000 is ideal for determining the size of double-stranded DNA from 250 to
10000bp base pa irs. The ladder consists of 7 linear double-stranded fragments.
The fragment is present at increased intensity to al low easy ident ificat ion.
Al l fragments are prec isely quantified and mixed during the production. For
5 l loading, al l fragments except 2500bp are 50ng. The 2500bp is 125ng. Thi s ladder
2500bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
250, 1000, , 5000, 7500, 10000, 150002500
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 85ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 125ng/ l, Other bands: 50ng/ l
DS 15000 Cat: M1161-50µg, M1162-5x50µgTM
TM
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E-mail: [email protected] Fax: +86-20-87791381 18
DNA Ladders & Markers
DS 2000 is ideal for determining the size of double-stranded DNA from 100 to
2000bp base pairs. The ladder consists of 6 linear double-stranded fragmen ts.
The fragmen t is present at increased intensity to allow easy identification.
Al l fragmen ts are precisely quantified and mixed during the production. For
5 l loading, all fragmen ts except 750bp are 75ng. The 750bp is 150ng. Th is ladder
750bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
100, 500, , 1000, 2000200, 750
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 105ng/ l
Recommended Electrophoresis Condition
8cm 1.7% Agarose Gel, 0.5x TBE, 7V/cm, 40min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 150ng/ l, Other bands: 75ng/ l
DS 2000 Cat: M1101-50µg, M1102-5x50µg
1.7
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TM
TM
DS 5000 is ideal for determining the size of double-stranded DNA from 100 to
5000bp base pa irs. The ladder consists of 9 linear double-stranded fragments.
The fragment is present at increased intensity to al low easy ident ificat ion.
Al l fragments are prec isely quantified and mixed during the production. For
5 l loading, al l fragments except 1000bp are 75ng. The 1500bp is 150ng. Thi s ladder
1000bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
100, 250, 500, 750, , 1500, 2000, 3000, 50001000
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 150ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 40min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 150ng/ l, Other bands: 75ng/ l
DS 5000 Cat: M1111-50µg, M1112-5x50µgTM
TM
1%
ag
aro
se
http://dongshengbio.com Tel: +86-20-8779135617
1
DN
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ad
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rs &
Ma
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rs
1%
ag
aro
se
1%
ag
aro
se
DS 10000 is ideal for det ermining the size of doubl e-st randed DNA from 250 to
10000bp base pai rs. The ladder cons ists of 7 linear doubl e-st randed fragm ent s.
The fragm ent is presen t at incr ease d intensi ty to al low easy ident ificat ion.
Al l fragm ent s are preci sel y quant ified and mixed dur ing the produc tion. For
5 l loadi ng, al l fragm ent s exc ept 2000bp are 50ng. The 2000bp is 125ng. Thi s ladder
2000bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
250, 1000, , 4000,7000, 10000500, 2000
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 85ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 125ng/ l, Other bands: 50ng/ l
DS 10000 Cat: M1221-50µg, M1222-5x50µgTM
TM
DS 15000 is ideal for determining the size of double-stranded DNA from 250 to
10000bp base pa irs. The ladder consists of 7 linear double-stranded fragments.
The fragment is present at increased intensity to al low easy ident ificat ion.
Al l fragments are prec isely quantified and mixed during the production. For
5 l loading, al l fragments except 2500bp are 50ng. The 2500bp is 125ng. Thi s ladder
2500bp
Description
is pre-mixed with loading dye and is ready to use.
Contents(bp)
250, 1000, , 5000, 7500, 10000, 150002500
Storage:
3 months at room temperature, 2 years at -20℃.
Concentration: 85ng/ l
Recommended Electrophoresis Condition
8cm 1% Agarose Gel, 1x TAE, 7V/cm, 45min
Recommended Loading : 2-5 l/lane
Concentration: Typical bands: 125ng/ l, Other bands: 50ng/ l
DS 15000 Cat: M1161-50µg, M1162-5x50µgTM
TM
1
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A L
ad
ers
& M
ark
ers
E-mail: [email protected] Fax: +86-20-87791381 18
DNA Ladders & Markers
TMDS is a collection of 30 highly-purified individual DNA fragmentsranging from 10-15,000 bp.
Custom DNA Ladders Service
We offer exceptionally pure DNA fragments that
are free of any truncated or degraded molecules.10 bp
15 bp
20 bp
25 bp
35 bp
50 bp
75 bp
100 bp
150 bp
200 bp
250 bp
300 bp
400 bp
500 bp
600 bp
700 bp
800 bp
900 bp
1000 bp
1200 bp
1500 bp
2000 bp
2500 bp
3000 bp
4000 bp
5000 bp
6000 bp
8000 bp
10000 bp
15000 bp
Storage Buffer (TE Buffer)
10 mM Tris-HCl (pH 7.6) with 1 mM EDTA
Quality Control
Tested on an Agilent 2100 bioanalyzer and by ap-
propriate gel electrophoresis. DNA concentration
is determined by spectrophotometer. The abse-
nce of nucleases confirmed by appropriate tests..
Storage
2 years at -20℃
Bulk orders can be customized to fit your needs:
Individual DNA fragments or mixtures.
Custom ladders of any combination and conce-
ntration.
Reference bands in a custom-made DNA ladder.
Flexible formulations.
Ready-to-use versions premixed with any load-
ing dye.
From microgram to gram quantities.
M8011
M8021
M8031
M8041
M8051
M8061
M8071
M8081
M8091
M8101
M8111
M8121
M8131
M8141
M8151
M8161
M8171
M8181
M8191
M8201
M8211
M8221
M8231
M8241
M8251
M8261
M8271
M8281
M8291
M8301
DNA Fragnent Size Cat # Amount
0.1
DNA Ladder/Marker Customized Service
http://dongshengbio.com Tel: +86-20-8779135619
1
DN
A L
ad
de
rs &
Ma
rke
rs
目录目录目录目录
PCR相关产品1 PCR概述 ---------------------------------------------1.1 PCR原理及示意图 -----------------------------------------------
1.2 PCR实验注意事项 -----------------------------------------------
1.3 PCR产品选择指南 -----------------------------------------------
1.4 PCR产品简介 ----------------------------------------------------
2 嗜热DNA聚合酶 ------------------------------------Taq DNA 聚合酶 -------------------------------------------------------
TM HS Taq DNA 聚合酶 -------------------------------------------------
Pfu DNA 聚合酶 -------------------------------------------------------
Taq Plus DNA 聚合酶 -------------------------------------------------
Long Taq DNA 聚合酶 -------------------------------------------------TM FS Taq DNA 聚合酶 --------------------------------------------------
3 PCR Mix ---------------------------------------------PCR Mix ----------------------------------------------------------------
TM HS Taq Mix -----------------------------------------------------------
Pfu Mix ------------------------------------------------------------------
Plus Mix ----------------------------------------------------------------
Long Taq Mix ----------------------------------------------------------
SYBR Green qPCR Mix ---------------------------------------------- +Power SYBR Green PCR Master Mix(Rox ) ----------------------
TM FS Mix -----------------------------------------------------------TM FS Mix Direct for Blood ---------------------------------------TM FS Mix Direct for Tissue --------------------------------------
4 PCR Kit ----------------------------------------------PCR Kit -----------------------------------------------------------------
TM HS Kit -----------------------------------------------------------------
Pfu Kit ------------------------------------------------------------------
Long Taq Kit -----------------------------------------------------------TM FS Kit -----------------------------------------------------------------
5 PCR教学试剂盒 - - - - - - - - - - - - - - - - - - - - - - - - - - - -500 bp PCR反应试剂盒 ----------------------------------------------
1000 bp PCR反应试剂盒 --------------------------------------------
6 重组菌落PCR检测试剂盒 -------------------------通用菌落PCR检测试剂盒 --------------------------------------------
7 PCR辅助试剂 ---------------------------------------dNTPs ------------------------------------------------------------------
超纯水 ------------------------------------------------------------------
25 mM MgCl ----------------------------------------------------------2
PCR Enhancer --------------------------------------------------------
10×PCR Buffer --------------------------------------------------------
PCR标本处理液 -------------------------------------------------------
8 PCR常见问题分析 ---------------------------------
2626
26
29
31
3232
34
36
38
40
42
4444
46
48
50
52
54
56
58
60
62
6464
66
68
70
72
7474
74
7575
7676
76
76
77
77
78
79
2. PCR, qPCR & RT-PCR
TMDS is a collection of 30 highly-purified individual DNA fragmentsranging from 10-15,000 bp.
Custom DNA Ladders Service
We offer exceptionally pure DNA fragments that
are free of any truncated or degraded molecules.10 bp
15 bp
20 bp
25 bp
35 bp
50 bp
75 bp
100 bp
150 bp
200 bp
250 bp
300 bp
400 bp
500 bp
600 bp
700 bp
800 bp
900 bp
1000 bp
1200 bp
1500 bp
2000 bp
2500 bp
3000 bp
4000 bp
5000 bp
6000 bp
8000 bp
10000 bp
15000 bp
Storage Buffer (TE Buffer)
10 mM Tris-HCl (pH 7.6) with 1 mM EDTA
Quality Control
Tested on an Agilent 2100 bioanalyzer and by ap-
propriate gel electrophoresis. DNA concentration
is determined by spectrophotometer. The abse-
nce of nucleases confirmed by appropriate tests..
Storage
2 years at -20℃
Bulk orders can be customized to fit your needs:
Individual DNA fragments or mixtures.
Custom ladders of any combination and conce-
ntration.
Reference bands in a custom-made DNA ladder.
Flexible formulations.
Ready-to-use versions premixed with any load-
ing dye.
From microgram to gram quantities.
M8011
M8021
M8031
M8041
M8051
M8061
M8071
M8081
M8091
M8101
M8111
M8121
M8131
M8141
M8151
M8161
M8171
M8181
M8191
M8201
M8211
M8221
M8231
M8241
M8251
M8261
M8271
M8281
M8291
M8301
DNA Fragnent Size Cat # Amount
0.1
DNA Ladder/Marker Customized Service
http://dongshengbio.com Tel: +86-20-8779135619
1
DN
A L
ad
de
rs &
Ma
rke
rs
目录目录目录目录
PCR相关产品1 PCR概述 ---------------------------------------------1.1 PCR原理及示意图 -----------------------------------------------
1.2 PCR实验注意事项 -----------------------------------------------
1.3 PCR产品选择指南 -----------------------------------------------
1.4 PCR产品简介 ----------------------------------------------------
2 嗜热DNA聚合酶 ------------------------------------Taq DNA 聚合酶 -------------------------------------------------------
TM HS Taq DNA 聚合酶 -------------------------------------------------
Pfu DNA 聚合酶 -------------------------------------------------------
Taq Plus DNA 聚合酶 -------------------------------------------------
Long Taq DNA 聚合酶 -------------------------------------------------TM FS Taq DNA 聚合酶 --------------------------------------------------
3 PCR Mix ---------------------------------------------PCR Mix ----------------------------------------------------------------
TM HS Taq Mix -----------------------------------------------------------
Pfu Mix ------------------------------------------------------------------
Plus Mix ----------------------------------------------------------------
Long Taq Mix ----------------------------------------------------------
SYBR Green qPCR Mix ---------------------------------------------- +Power SYBR Green PCR Master Mix(Rox ) ----------------------
TM FS Mix -----------------------------------------------------------TM FS Mix Direct for Blood ---------------------------------------TM FS Mix Direct for Tissue --------------------------------------
4 PCR Kit ----------------------------------------------PCR Kit -----------------------------------------------------------------
TM HS Kit -----------------------------------------------------------------
Pfu Kit ------------------------------------------------------------------
Long Taq Kit -----------------------------------------------------------TM FS Kit -----------------------------------------------------------------
5 PCR教学试剂盒 - - - - - - - - - - - - - - - - - - - - - - - - - - - -500 bp PCR反应试剂盒 ----------------------------------------------
1000 bp PCR反应试剂盒 --------------------------------------------
6 重组菌落PCR检测试剂盒 -------------------------通用菌落PCR检测试剂盒 --------------------------------------------
7 PCR辅助试剂 ---------------------------------------dNTPs ------------------------------------------------------------------
超纯水 ------------------------------------------------------------------
25 mM MgCl ----------------------------------------------------------2
PCR Enhancer --------------------------------------------------------
10×PCR Buffer --------------------------------------------------------
PCR标本处理液 -------------------------------------------------------
8 PCR常见问题分析 ---------------------------------
2626
26
29
31
3232
34
36
38
40
42
4444
46
48
50
52
54
56
58
60
62
6464
66
68
70
72
7474
74
7575
7676
76
76
77
77
78
79
2. PCR, qPCR & RT-PCR
http://dongshengbio.com Tel: +86-20-87791356
PCR (polymerase chain reaction) is a technique that is central to molec-
ular biology research. It is used in applications such as cloning, gene ex-
DNA amplification.
Dongsheng Biotech offers different series of products to help you achi-TM
eve PCR success. For enzymes, our Taq Polymerase, HS Taq DNATM
Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and
Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity
PCR performance. We also have Long Taq DNA Polymerase for long
We provi de four ser ies of PCR product s for di fferent uses:
PCR Polymerase Series
PCR Mix Series
PCR Kit Ser ies
qPCR Mix Series
RT-PCR Series
PC
R, q
PC
R &
RT
-PC
R
2
21
pression analysis, genotyping, sequencing, and mutant genes.
PCR Products
PCR, qPCR & RT-PCR
E-mail: [email protected] Fax: +86-20-87791381
Propertie s of PCR enzymes
HS TaqTM
TMFS Taq
Enzyme
Taq
Pfu
Feature Application
General PCR/RT-PCR
DNA Mark/DNA Sequence
TA Clone
++
++
Fidelity
+ ++
PCR Product ends
3'-dA+++
+ ++++ 3'-dA
+++ + ++++ 3'-dA
5'→3' Activity
5'? 3' Activity
5'→3'Activity
5'→3' Activity
Fast-3 times of Taq
Amplification size
High fidelity PCR
Blunt end vector cloning
RT-PCR/cDNA clone and
express/SNP
+++++ +++
5'-3' Exonclease activity
Taq Plus
LongTaq
Fusion pfu High fidelity PCR
Long fragment PCR
++++
+++++++++
+++
++++++++
+++
++
++++
3'-dA/
Blunt end
GC rich template
Complicated template
High repeat sequencesHigh fidelity-4 times of Taq
Blunt end
General PCR/RT-PCR
DNA Mark/DNA Sequence
TA Clone
5'-3' Exonclease activity
5'? 3' Activity
High fidelity-4 times of Taq
5'→3' Activity
5'-3' Exonclease activity3'-dA/
Blunt end
GC rich template
Long template
Sequencing
Blunt end5'? 3' Activity
5'-3' Exonclease activity
Sensivity
PC
R, q
PC
R &
RT
-PC
R
2
22
General PCR/RT-PCR
DNA Mark/DNA Sequence
TA Clone
More and more convenient
Mo
re a
nd
mo
re re
find
fun
tion Cored by genetic engineering technology
Based by Taq DNA Polymerase
http://dongshengbio.com Tel: +86-20-87791356
PCR (polymerase chain reaction) is a technique that is central to molec-
ular biology research. It is used in applications such as cloning, gene ex-
DNA amplification.
Dongsheng Biotech offers different series of products to help you achi-TM
eve PCR success. For enzymes, our Taq Polymerase, HS Taq DNATM
Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and
Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity
PCR performance. We also have Long Taq DNA Polymerase for long
We provi de four ser ies of PCR product s for di fferent uses:
PCR Polymerase Series
PCR Mix Series
PCR Kit Ser ies
qPCR Mix Series
RT-PCR Series
PC
R, q
PC
R &
RT
-PC
R
2
21
pression analysis, genotyping, sequencing, and mutant genes.
PCR Products
PCR, qPCR & RT-PCR
E-mail: [email protected] Fax: +86-20-87791381
Propertie s of PCR enzymes
HS TaqTM
TMFS Taq
Enzyme
Taq
Pfu
Feature Application
General PCR/RT-PCR
DNA Mark/DNA Sequence
TA Clone
++
++
Fidelity
+ ++
PCR Product ends
3'-dA+++
+ ++++ 3'-dA
+++ + ++++ 3'-dA
5'→3' Activity
5'? 3' Activity
5'→3'Activity
5'→3' Activity
Fast-3 times of Taq
Amplification size
High fidelity PCR
Blunt end vector cloning
RT-PCR/cDNA clone and
express/SNP
+++++ +++
5'-3' Exonclease activity
Taq Plus
LongTaq
Fusion pfu High fidelity PCR
Long fragment PCR
++++
+++++++++
+++
++++++++
+++
++
++++
3'-dA/
Blunt end
GC rich template
Complicated template
High repeat sequencesHigh fidelity-4 times of Taq
Blunt end
General PCR/RT-PCR
DNA Mark/DNA Sequence
TA Clone
5'-3' Exonclease activity
5'? 3' Activity
High fidelity-4 times of Taq
5'→3' Activity
5'-3' Exonclease activity3'-dA/
Blunt end
GC rich template
Long template
Sequencing
Blunt end5'? 3' Activity
5'-3' Exonclease activity
Sensivity
PC
R, q
PC
R &
RT
-PC
R
2
22
General PCR/RT-PCR
DNA Mark/DNA Sequence
TA Clone
More and more convenient
Mo
re a
nd
mo
re re
find
fun
tion Cored by genetic engineering technology
Based by Taq DNA Polymerase
http://dongshengbio.com Tel: +86-20-87791356
Product Name Cat # Size Description
Taq DNA Polymerase
TM HS Taq DNA Polymerase
Pfu DNA Polymerase
Taq Plus DNA Polymerase
Long Taq DNA Polymerase
TMFS Taq DNA Polymerase
P1011
P1012*
P1013
P1014*
P1015
P1081
P1082
P1083
P1084
P1021
P1022*
P1023
P1024*
P1031
P1032*
P1033
P1034*
P1061
P1062*
P1063
P1064*
P1071
P1072*
P1073
P1074*
500U
500U
1,000U
1,000U
18,000U
250U
500U
1,000U
18,000U
250U
250U
1,000U
1,000U
250U
250U
1,000U
1,000U
250U
250U
1,000U
1,000U
250U
250U
1,000U
1,000U
Robust Taq DNA Polymerase for routine
use incl.10x PCR Buffer, 6xloading buffer,
Mg2+ plus or free. Supply with 5U/ and
2.5U/ packages.
µl
µl
TM High specificity, incl. 10x HS PCR buffer,
6x loading buffer, Mg2+ plus or free.
Supply with 5U/ and 2.5U/ two
packages.
µl µl
High fidelity, incl.10x pfu buffer,
6xloading buffer, Mg2+ plus or free.
Supply with 5U/ and 2.5U/ two
packages.
µl µl
Mix with Taq Polymerase and Pfu
Polymerase, long amplification and
high fidelity. Incl. 10xTaq Plus buffer,
Mg2+ plus or free. Supply with 5U/
and 2.5U/ two packages.
µl
µl
For long PCR, incl. 10xLong Taq buffer,
6xloading buffer, Mg2+ plus or free.
Supply with 5U/ and 2.5U two
packages.
µl /µl
TMFast, incl. 10xFS PCR buffer, 6xloading
buffer, Mg2+ plus or free. Supply with
5U/ and 2.5U/ two packages.µl µl
Taq Mix (2x)
TM HS Mix (2x)
Pfu Mix (2x)
P2011
P2011
P2081
P2082
P2021
P2022
1ml
5x1ml
1ml
5x1ml
1ml
5x1ml
Pre mix with Taq DNA Polymerase, dNTPs,
Mg2+ and Reaction buffer at optional conc-
entrations
TM Pre mix with HS Taq DNA Polymerase,
dNTPs, Mg2+ and Reaction buffer at optional
concentrations
. Pre mix with Pfu DNA Polymerase, dNTPs,
Mg2+ and Reaction buffer at optional conc-
entrations.
PC
R, q
PC
R &
RT
-PR
2
23
TMFS Taq Mix Direct for Blood (2x)
TM FS Taq Mix Direct for Tissue (2x)
P3021
P3022
P3031
P3032
1ml
5x1ml
1ml
5x1ml
Pre mix with Long Taq DNA Polymerase, dNTPs,
Mg2+ and Reaction buffer at optional concentrations.
TM Pre mix with FS Taq DNA Polymerase, dNTPs, Mg2+
and Reaction buffer at optional concentrations.
TM Contains FS Taq Mix T(2x) and sample preparation
solution (Extraction solution and Neutralization solution
PCR, qPCR & RT-PCR
Long Mix (2x)P2061
P2062
1ml
5x1ml
TM FS Mix (2x)P3011
P3012
1ml
5x1m
TM Pre mix with FS Taq DNA Polymerase, dNTPs, Mg2+
and Reaction buffer at optional concentrations.
Plus Mix (2x) P2031
P2031
1ml
5x1ml
Pre mix with Taq Plus DNA Polymerase, dNTPs,
Mg2+ and Reaction buffer at optional concentrations.
PCR Products
E-mail: [email protected] Fax: +86-20-87791381
dNTP Mix (2.5mM)
dNTP Mix (10mM)
dATP (100mM)
dTTP (100mM)
dCTP (100mM)
dGTP (100mM)
P9011
P9012
P9013
P9071
P9081
P9091
P9101
1ml
0.5ml
1ml
0.5ml
0.5ml
0.5ml
0.5ml
Contains 250U Taq DNA Polymerase and
5x1ml CR Reaction Mix (2x)
Contains 250U HS Taq DNA Polymerase
and 5x1ml HS Reaction Mix (2x)
Contains 250U FS Taq DNA Polymerase
and 5x1ml FS Reaction Mix (2x)
Contains 250U Long Taq DNA Polymerase
and 5x1ml Long Reaction Mix (2x)
With 15mM Mg2+
Without 15mM Mg2+
With Mg2+ concentration set
To prepare blood or tissue sample for
fast PCR
PC
R, q
PC
R &
RT
-PC
Rs
2
PCR, qPCR & RT-PCR
24
PCR Kit
TMHS Kit
Pfu Kit
Long Taq Kit
TMFS Kit
P3012
P3082
P3022
P3062
P3072
5x1ml
5x1ml
5x1ml
5x1ml
5x1ml
Contains 250U Pfu DNA Polymerase
and 5x1ml Pfu Reaction Mix (2x)
10xPCR buffer
MgCl (25mM)2
Nuclease-free Water
PCR Enhancer
PCR Sample Preparation Solution
P5011
P5011a
P5011b
P9031
P9021
P9022
P9023
P9041
P9051
P9052
4x1.25ml
4x1.25ml
6x1ml
4x1.25ml
5x1ml
100ml
500ml
500ul
50 preps
250 preps
Product Name Cat # Size Description
RT-PCR Products
Product Name Cat # Size Description
Mix reaction (2x) for real-time PCR
With low Rox+
With high Rox+
SYBR Green qPCR Mix
P2091
P2092
P2091a
P2092a
P2091b
P2092b
1ml
5x1ml
1ml
5x1ml
1ml
5x1ml
qPCR Products
Product Name Cat # Size Description
®
®SYBR Green qPCR Mix (Rox )+
M-MLV (recombinant)
RT-PCR
RNasin (RNase inhibitor)
Oligo d(T) Primer15
Random Primer (6mer)
DEPC-treated Water
RNA Stabilization Solution
R1041
R1042
R1011
R1012
R2011
R2021
R2031
R2041
R2042
R2071
R2072
5000U
10000U
20 rxns
100 rxns
1000U
20µl
20µl
30ml
100ml
20ml
100ml
http://dongshengbio.com Tel: +86-20-87791356
Product Name Cat # Size Description
Taq DNA Polymerase
TM HS Taq DNA Polymerase
Pfu DNA Polymerase
Taq Plus DNA Polymerase
Long Taq DNA Polymerase
TMFS Taq DNA Polymerase
P1011
P1012*
P1013
P1014*
P1015
P1081
P1082
P1083
P1084
P1021
P1022*
P1023
P1024*
P1031
P1032*
P1033
P1034*
P1061
P1062*
P1063
P1064*
P1071
P1072*
P1073
P1074*
500U
500U
1,000U
1,000U
18,000U
250U
500U
1,000U
18,000U
250U
250U
1,000U
1,000U
250U
250U
1,000U
1,000U
250U
250U
1,000U
1,000U
250U
250U
1,000U
1,000U
Robust Taq DNA Polymerase for routine
use incl.10x PCR Buffer, 6xloading buffer,
Mg2+ plus or free. Supply with 5U/ and
2.5U/ packages.
µl
µl
TM High specificity, incl. 10x HS PCR buffer,
6x loading buffer, Mg2+ plus or free.
Supply with 5U/ and 2.5U/ two
packages.
µl µl
High fidelity, incl.10x pfu buffer,
6xloading buffer, Mg2+ plus or free.
Supply with 5U/ and 2.5U/ two
packages.
µl µl
Mix with Taq Polymerase and Pfu
Polymerase, long amplification and
high fidelity. Incl. 10xTaq Plus buffer,
Mg2+ plus or free. Supply with 5U/
and 2.5U/ two packages.
µl
µl
For long PCR, incl. 10xLong Taq buffer,
6xloading buffer, Mg2+ plus or free.
Supply with 5U/ and 2.5U two
packages.
µl /µl
TMFast, incl. 10xFS PCR buffer, 6xloading
buffer, Mg2+ plus or free. Supply with
5U/ and 2.5U/ two packages.µl µl
Taq Mix (2x)
TM HS Mix (2x)
Pfu Mix (2x)
P2011
P2011
P2081
P2082
P2021
P2022
1ml
5x1ml
1ml
5x1ml
1ml
5x1ml
Pre mix with Taq DNA Polymerase, dNTPs,
Mg2+ and Reaction buffer at optional conc-
entrations
TM Pre mix with HS Taq DNA Polymerase,
dNTPs, Mg2+ and Reaction buffer at optional
concentrations
. Pre mix with Pfu DNA Polymerase, dNTPs,
Mg2+ and Reaction buffer at optional conc-
entrations.
PC
R, q
PC
R &
RT
-PR
2
23
TMFS Taq Mix Direct for Blood (2x)
TM FS Taq Mix Direct for Tissue (2x)
P3021
P3022
P3031
P3032
1ml
5x1ml
1ml
5x1ml
Pre mix with Long Taq DNA Polymerase, dNTPs,
Mg2+ and Reaction buffer at optional concentrations.
TM Pre mix with FS Taq DNA Polymerase, dNTPs, Mg2+
and Reaction buffer at optional concentrations.
TM Contains FS Taq Mix T(2x) and sample preparation
solution (Extraction solution and Neutralization solution
PCR, qPCR & RT-PCR
Long Mix (2x)P2061
P2062
1ml
5x1ml
TM FS Mix (2x)P3011
P3012
1ml
5x1m
TM Pre mix with FS Taq DNA Polymerase, dNTPs, Mg2+
and Reaction buffer at optional concentrations.
Plus Mix (2x) P2031
P2031
1ml
5x1ml
Pre mix with Taq Plus DNA Polymerase, dNTPs,
Mg2+ and Reaction buffer at optional concentrations.
PCR Products
E-mail: [email protected] Fax: +86-20-87791381
dNTP Mix (2.5mM)
dNTP Mix (10mM)
dATP (100mM)
dTTP (100mM)
dCTP (100mM)
dGTP (100mM)
P9011
P9012
P9013
P9071
P9081
P9091
P9101
1ml
0.5ml
1ml
0.5ml
0.5ml
0.5ml
0.5ml
Contains 250U Taq DNA Polymerase and
5x1ml CR Reaction Mix (2x)
Contains 250U HS Taq DNA Polymerase
and 5x1ml HS Reaction Mix (2x)
Contains 250U FS Taq DNA Polymerase
and 5x1ml FS Reaction Mix (2x)
Contains 250U Long Taq DNA Polymerase
and 5x1ml Long Reaction Mix (2x)
With 15mM Mg2+
Without 15mM Mg2+
With Mg2+ concentration set
To prepare blood or tissue sample for
fast PCR
PC
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PCR, qPCR & RT-PCR
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PCR Kit
TMHS Kit
Pfu Kit
Long Taq Kit
TMFS Kit
P3012
P3082
P3022
P3062
P3072
5x1ml
5x1ml
5x1ml
5x1ml
5x1ml
Contains 250U Pfu DNA Polymerase
and 5x1ml Pfu Reaction Mix (2x)
10xPCR buffer
MgCl (25mM)2
Nuclease-free Water
PCR Enhancer
PCR Sample Preparation Solution
P5011
P5011a
P5011b
P9031
P9021
P9022
P9023
P9041
P9051
P9052
4x1.25ml
4x1.25ml
6x1ml
4x1.25ml
5x1ml
100ml
500ml
500ul
50 preps
250 preps
Product Name Cat # Size Description
RT-PCR Products
Product Name Cat # Size Description
Mix reaction (2x) for real-time PCR
With low Rox+
With high Rox+
SYBR Green qPCR Mix
P2091
P2092
P2091a
P2092a
P2091b
P2092b
1ml
5x1ml
1ml
5x1ml
1ml
5x1ml
qPCR Products
Product Name Cat # Size Description
®
®SYBR Green qPCR Mix (Rox )+
M-MLV (recombinant)
RT-PCR
RNasin (RNase inhibitor)
Oligo d(T) Primer15
Random Primer (6mer)
DEPC-treated Water
RNA Stabilization Solution
R1041
R1042
R1011
R1012
R2011
R2021
R2031
R2041
R2042
R2071
R2072
5000U
10000U
20 rxns
100 rxns
1000U
20µl
20µl
30ml
100ml
20ml
100ml
Taq DNA Polymerase -routine Taq
Storage:
2 years at -20℃.
Code
P1011
P1012*
P1013
P1014*
P1015
Size
500U
500U
1000U
1000U
18,000U
* means supplied with dNTPs.
Description
Taq DNA Polymerase is a themostable recombinant DNA Polymerase derived
from Thermos aquaticus. It is a robust Taq for routine PCR use. Its molecular
weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5kb
(simple template). The elongation velocity is 0.9~1.2kb/min (70~75℃ ). It has
5' ? 3' exonuclease activity but lacks 3' ? 5’exonuclease activity that results
in a 3'-dA overhangs PCR product.
Description
Storage:
2 years at -20℃.
Contents:
Taq DNA Polymerase(5U/ l), 10xPCR Buffer, 6xLoading buffer,
dNTPs*(P1012, P1014)
Code
P1081
P1082
P1083
P1084
Size
250U
500U
1000U
18,000U
Contents:
, 10x PCR Buffer, 6xLoading bufferTM TM HS Taq DNA Polymerase(5U/ l) HS
TMHS Taq DNA Polymerase -high specificity
TM HS Taq DNA Polymer ase is a thermos table recombi nant DNA Polymer ase
derived from Thermos aquaticus. It contents the different reaction buffer that
is designed to provide highly specific ampl ification of nucleic acid templ ates
by PCR . The elongation velocity is 0.9~1.2kb/min .It is heat-stable
and wi ll synthesize DNA at elevated
(70~75 )
tempe ratures from single-stranded templ ates
Pfu DNA Polymerase -high fidelity
Storage:
2 years at -20℃.
Code
P1021
P1022*
P1023
P1024*
Size
250U
250U
1000U
1000U
* means supplied with dNTPs.
Description
Pfu DNA Polymerase has superior thermostability and proofreading properties
compared to other thermostable polymerase. It can amplify DNA target up to
2kb (simple template). Its fidelity can reach 10 time of Taq DNA Polymerase.
The elongation velocity is 0.2~0.4kb/min (70~75℃ ). It results in blunt-ended
PCR products, which are ideal for cloning into blunt-ended vectors.Pru DNA
Polymerase is superior for techniques that require high-fidelity DNA synthesis.
Contents:
Pfu DNA Polymerase(2.5U/ l), 10xPfu Buffer, 6xLoading buffer,
dNTPs* (P1022, P1024)
in the presence of a primer.
http://dongshengbio.com Tel: +86-20-87791356
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Taq Plus DNA Polymerase -high fidelity, high efficiency
Storage:
2 years at -20℃.
Code
P1031
P1032*
P1033
P1034*
Size
250U
250U
1000U
1000U
* means supplied with dNTPs.
Description
Taq Plus DNA Polymerase is a mixture of Taq and Pfu polymerase, blends the
processivity of taq with the high fidelity of pfu. The two enzymes act synergis-
tically during PCR to generate more accurate, longer and greater yield PCR
products. It can amplify DNA target up to 20kb (simple template).
The error rate
Description
Storage:
2 years at -20℃.
Contents:
Taq Plus DNA Polymerase(2.5U/ l), 10xPCR Buffer, 6xLoading buffer,
dNTPs*(P1032, P1034)
Code
P1061
P1062*
P1063
P1064
Size
250U
250U
1000U
1000U
Contents:
, 10x Long PCR Buffer I, 10x Long PCR Buffer II
PCR Enhancer, 6xLoading buffer
Long Taq DNA Polymerase(5U/ l)
Long Taq DNA Polymerase -for complex templates
Long Taq DNA Polymerase, a combination of Taq and Pfu, is a special formulation
desigen for amplifying large fragment. This specially formulated Long Taq was
shown to amplify long templates from λ phage genome of up to 20 kb. It is also a
better choice for amplifying , such as . It will
complex templates GC-rich templates
TMFS Taq DNA Polymerase
Storage:
2 years at -20℃.
Code
P1071
P1072*
P1073
P1074*
Size
250U
250U
1000U
1000U
* means supplied with dNTPs.
Description TM FS Taq DNA Polymerase, developed by Dongsheng Biotech, is the latst
generation Taq-based DNA Polymerase. It processes high amplification
efficiency as Taq polymerase does, and fast elongation ability as KOD
polymerase does,can be used as a variety of PCR. The elongation rate of TM FS Taq is 2 times higher than regular Taq. It will save the amplification time
Contents:
, 10x PCR Buffer, 6xLoading bufferTM TM FS Taq DNA Polymerase(5U/ l) FS
result in 3’-dA overhangs PCR products, which can be used In TA clone.
-fast Taq
-5is 7.5x10 per nucleotide per cycle.
by half.
PCR, qPCR & RT-PCR
E-mail: [email protected] Fax: +86-20-87791381
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Taq DNA Polymerase -routine Taq
Storage:
2 years at -20℃.
Code
P1011
P1012*
P1013
P1014*
P1015
Size
500U
500U
1000U
1000U
18,000U
* means supplied with dNTPs.
Description
Taq DNA Polymerase is a themostable recombinant DNA Polymerase derived
from Thermos aquaticus. It is a robust Taq for routine PCR use. Its molecular
weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5kb
(simple template). The elongation velocity is 0.9~1.2kb/min (70~75℃ ). It has
5' ? 3' exonuclease activity but lacks 3' ? 5’exonuclease activity that results
in a 3'-dA overhangs PCR product.
Description
Storage:
2 years at -20℃.
Contents:
Taq DNA Polymerase(5U/ l), 10xPCR Buffer, 6xLoading buffer,
dNTPs*(P1012, P1014)
Code
P1081
P1082
P1083
P1084
Size
250U
500U
1000U
18,000U
Contents:
, 10x PCR Buffer, 6xLoading bufferTM TM HS Taq DNA Polymerase(5U/ l) HS
TMHS Taq DNA Polymerase -high specificity
TM HS Taq DNA Polymer ase is a thermos table recombi nant DNA Polymer ase
derived from Thermos aquaticus. It contents the different reaction buffer that
is designed to provide highly specific ampl ification of nucleic acid templ ates
by PCR . The elongation velocity is 0.9~1.2kb/min .It is heat-stable
and wi ll synthesize DNA at elevated
(70~75 )
tempe ratures from single-stranded templ ates
Pfu DNA Polymerase -high fidelity
Storage:
2 years at -20℃.
Code
P1021
P1022*
P1023
P1024*
Size
250U
250U
1000U
1000U
* means supplied with dNTPs.
Description
Pfu DNA Polymerase has superior thermostability and proofreading properties
compared to other thermostable polymerase. It can amplify DNA target up to
2kb (simple template). Its fidelity can reach 10 time of Taq DNA Polymerase.
The elongation velocity is 0.2~0.4kb/min (70~75℃ ). It results in blunt-ended
PCR products, which are ideal for cloning into blunt-ended vectors.Pru DNA
Polymerase is superior for techniques that require high-fidelity DNA synthesis.
Contents:
Pfu DNA Polymerase(2.5U/ l), 10xPfu Buffer, 6xLoading buffer,
dNTPs* (P1022, P1024)
in the presence of a primer.
http://dongshengbio.com Tel: +86-20-87791356
PC
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RT
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Taq Plus DNA Polymerase -high fidelity, high efficiency
Storage:
2 years at -20℃.
Code
P1031
P1032*
P1033
P1034*
Size
250U
250U
1000U
1000U
* means supplied with dNTPs.
Description
Taq Plus DNA Polymerase is a mixture of Taq and Pfu polymerase, blends the
processivity of taq with the high fidelity of pfu. The two enzymes act synergis-
tically during PCR to generate more accurate, longer and greater yield PCR
products. It can amplify DNA target up to 20kb (simple template).
The error rate
Description
Storage:
2 years at -20℃.
Contents:
Taq Plus DNA Polymerase(2.5U/ l), 10xPCR Buffer, 6xLoading buffer,
dNTPs*(P1032, P1034)
Code
P1061
P1062*
P1063
P1064
Size
250U
250U
1000U
1000U
Contents:
, 10x Long PCR Buffer I, 10x Long PCR Buffer II
PCR Enhancer, 6xLoading buffer
Long Taq DNA Polymerase(5U/ l)
Long Taq DNA Polymerase -for complex templates
Long Taq DNA Polymerase, a combination of Taq and Pfu, is a special formulation
desigen for amplifying large fragment. This specially formulated Long Taq was
shown to amplify long templates from λ phage genome of up to 20 kb. It is also a
better choice for amplifying , such as . It will
complex templates GC-rich templates
TMFS Taq DNA Polymerase
Storage:
2 years at -20℃.
Code
P1071
P1072*
P1073
P1074*
Size
250U
250U
1000U
1000U
* means supplied with dNTPs.
Description TM FS Taq DNA Polymerase, developed by Dongsheng Biotech, is the latst
generation Taq-based DNA Polymerase. It processes high amplification
efficiency as Taq polymerase does, and fast elongation ability as KOD
polymerase does,can be used as a variety of PCR. The elongation rate of TM FS Taq is 2 times higher than regular Taq. It will save the amplification time
Contents:
, 10x PCR Buffer, 6xLoading bufferTM TM FS Taq DNA Polymerase(5U/ l) FS
result in 3’-dA overhangs PCR products, which can be used In TA clone.
-fast Taq
-5is 7.5x10 per nucleotide per cycle.
by half.
PCR, qPCR & RT-PCR
E-mail: [email protected] Fax: +86-20-87791381
PC
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RT
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Taq Mix (2x) -routine PCR
Storage:
2 years at -20℃.
Code
P2011
P2012
Size
1ml
5x1ml
Description
Description
Storage:
2 years at -20℃.
Contents: 2xTaq Mix, Nuclease-free Water
Code
P2081
P2082
Size
1ml
5x1ml
Contents: 2x TMHS Mix, Nuclease-free Water
TMHS Mix (2x) -high specificity
Pfu Mix (2x) -high fidelity
Storage:
2 years at -20℃.
Code
P2021
P2022
Size
1ml
5x1ml
Description
Pfu Mix (2x) is a premixed, ready-to-use solution containing Pfu Taq DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for
efficient PCR amplification. To prepare the final PCR, only primers and template
DNA are added. Mix contributes to highly reproducible PCR by reducing the
risk of pipetting errors, miscalculation and contamination. It also contributes to
Pfu
Contents: 2x Pfu Mix, Nuclease-free Water
Taq Mix (2x) is a premixed, ready-to-use solution containing Taq DNA Polym-2+erase, dNTPs, Mg and Reaction Buffer at optional concentrations for efficient
PCR amplification. To prepare the final PCR, only primers and template DNA
are added. This pre-mixed formulation saves time and reduces contamination
due to the fewer pipetting steps required for PCR set up. The mix retains all
features of Taq DNA Polymerase.
TM TM HS HS
TM HS
Mix (2x) is a premixed, ready-to-use solution containing Taq DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for
efficient PCR amplification. To prepare the final PCR, only primers and template
DNA are added. Mix provides increased sensitivity, specificity, and yield,
while allowing assembly of reactions at RT. This mix contributes to highly rep-
roducible PCR by reducing the risk of pipetting errors, miscalculation and
contamination. It is convenient and also save time.
higher sensitivity by adding intersifier and optimizer. Pfu Taq is superior for tech-
nique that requires high fidelity DNA synthesis.
http://dongshengbio.com Tel: +86-20-87791356
PC
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Plus Mix (2x) -high fidelity, high efficiency
Storage:
2 years at -20℃.
Code
P2031
P2032
Size
1ml
5x1ml
Description
Description
Storage:
2 years at -20℃.
Contents: 2xPlus Mix, Nuclease-free Water
Code
P2061
P2062
Size
1ml
5x1ml
Contents: 2xLong Taq Mix, Nuclease-free Water
Long Taq Mix (2x) -for complex templates
TMFS Mix (2x) -fast PCR
Storage:
2 years at -20℃.
Code
P2071
P2072
Size
1ml
5x1ml
Description
Contents: 2x Mix, Nuclease-free WaterTMFS
Plus Mix (2x) is a premixed, ready-to-use solution containing Taq Plus DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for
efficient PCR amplification. To prepare the final PCR, only primers and template
DNA are added. Plus Mis contributes to highly reproducible PCR by reducing
the risk of pipetting errors, miscalculation and contamination. It contains both
Long Taq Mix (2x) is a premixed, ready-to-use solution containing Long Taq DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for
efficient PCR amplification. To prepare the final PCR, only primers and template
DNA are added. Plus Mis contributes to highly reproducible PCR by reducing
the risk of pipetting errors, miscalculation and contamination. It also contributes
higher sensitivity by adding enhancer. It is good for complex templates, such as
GC-rich templates.
TM TM FS FS
The elongation rate of
.
Mix (2x) is a premixed, ready-to-use solution containing Taq DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for
efficient PCR amplification. To prepare the final PCR, only primers and template
DNA are added.This mix contributes to highly reproducible PCR by reducing the
risk of pipetting errors, miscalculation and contamination.
PCR, qPCR & RT-PCR
E-mail: [email protected] Fax: +86-20-87791381
PC
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TM FS Taq is 2 times higher than regular Taq
Taq Mix (2x) -routine PCR
Storage:
2 years at -20℃.
Code
P2011
P2012
Size
1ml
5x1ml
Description
Description
Storage:
2 years at -20℃.
Contents: 2xTaq Mix, Nuclease-free Water
Code
P2081
P2082
Size
1ml
5x1ml
Contents: 2x TMHS Mix, Nuclease-free Water
TMHS Mix (2x) -high specificity
Pfu Mix (2x) -high fidelity
Storage:
2 years at -20℃.
Code
P2021
P2022
Size
1ml
5x1ml
Description
Pfu Mix (2x) is a premixed, ready-to-use solution containing Pfu Taq DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for
efficient PCR amplification. To prepare the final PCR, only primers and template
DNA are added. Mix contributes to highly reproducible PCR by reducing the
risk of pipetting errors, miscalculation and contamination. It also contributes to
Pfu
Contents: 2x Pfu Mix, Nuclease-free Water
Taq Mix (2x) is a premixed, ready-to-use solution containing Taq DNA Polym-2+erase, dNTPs, Mg and Reaction Buffer at optional concentrations for efficient
PCR amplification. To prepare the final PCR, only primers and template DNA
are added. This pre-mixed formulation saves time and reduces contamination
due to the fewer pipetting steps required for PCR set up. The mix retains all
features of Taq DNA Polymerase.
TM TM HS HS
TM HS
Mix (2x) is a premixed, ready-to-use solution containing Taq DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for
efficient PCR amplification. To prepare the final PCR, only primers and template
DNA are added. Mix provides increased sensitivity, specificity, and yield,
while allowing assembly of reactions at RT. This mix contributes to highly rep-
roducible PCR by reducing the risk of pipetting errors, miscalculation and
contamination. It is convenient and also save time.
higher sensitivity by adding intersifier and optimizer. Pfu Taq is superior for tech-
nique that requires high fidelity DNA synthesis.
http://dongshengbio.com Tel: +86-20-87791356
PC
R, q
PC
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RT
-PC
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2
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Plus Mix (2x) -high fidelity, high efficiency
Storage:
2 years at -20℃.
Code
P2031
P2032
Size
1ml
5x1ml
Description
Description
Storage:
2 years at -20℃.
Contents: 2xPlus Mix, Nuclease-free Water
Code
P2061
P2062
Size
1ml
5x1ml
Contents: 2xLong Taq Mix, Nuclease-free Water
Long Taq Mix (2x) -for complex templates
TMFS Mix (2x) -fast PCR
Storage:
2 years at -20℃.
Code
P2071
P2072
Size
1ml
5x1ml
Description
Contents: 2x Mix, Nuclease-free WaterTMFS
Plus Mix (2x) is a premixed, ready-to-use solution containing Taq Plus DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for
efficient PCR amplification. To prepare the final PCR, only primers and template
DNA are added. Plus Mis contributes to highly reproducible PCR by reducing
the risk of pipetting errors, miscalculation and contamination. It contains both
Long Taq Mix (2x) is a premixed, ready-to-use solution containing Long Taq DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for
efficient PCR amplification. To prepare the final PCR, only primers and template
DNA are added. Plus Mis contributes to highly reproducible PCR by reducing
the risk of pipetting errors, miscalculation and contamination. It also contributes
higher sensitivity by adding enhancer. It is good for complex templates, such as
GC-rich templates.
TM TM FS FS
The elongation rate of
.
Mix (2x) is a premixed, ready-to-use solution containing Taq DNA 2+Polymerase, dNTPs, Mg and Reaction Buffer at optional concentrations for
efficient PCR amplification. To prepare the final PCR, only primers and template
DNA are added.This mix contributes to highly reproducible PCR by reducing the
risk of pipetting errors, miscalculation and contamination.
PCR, qPCR & RT-PCR
E-mail: [email protected] Fax: +86-20-87791381
PC
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TM FS Taq is 2 times higher than regular Taq
prepared sample
Code
P2071a
P2072a
Size
1ml
5x1ml
Description
TMFS Mix Direct for Blood (2x)
Storage:
2 years at -20℃.
Contents: 2x Mix B, Nuclease-free WaterTMFS
TMFSTM FS
Mix Direct for Blood (2x) is a premixed, ready-to-use solution
containing Taq DNA Polymerase, dNTPs, and all other PCR
components, except DNA template and primers. This mix is specific
for whole blood amplification. It contribute to fast, specific, sensitive
and reproducible PCR by reducing the fisk of contributes to highly
reproducible PCR by reducing the risk of pipetting errors, miscalc-
ulation and contamination. This product cut off the process of com-
TMFS Mix Direct for Tissue (2x)
Contents: 2x Mix T, Extraction Solution, Neutralization Solution
Nuclease-free Water
TMFS
whole or diluted blood
2x Mix B TMFS
primers water
plicated and waste time DNA extraction.
Code
P2071b
P2072b
Size
1ml
5x1ml
Description
Storage:
2 years at -20℃.
TMFSTM FS
Mix Direct for Tissue (2x) is a premixed, ready-to-use solution
containing Taq DNA Polymerase, dNTPs, and all other PCR
components, except DNA template and primers. This mix is specific
for tissue direct amplification. It contribute to fast, specific, sensitive
and reproducible PCR by reducing the fisk of contributes to highly
reproducible PCR by reducing the risk of pipetting errors, miscalc-
ulation and contamination. This product cut off the process of com-
plicated and waste time DNA extraction.
Sample preparation
2x Mix T TMFS
primers water
http://dongshengbio.com Tel: +86-20-87791356
PC
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PCR Kit -routine PCR
Storage:
2 years at -20℃.
Code
P3012
Size
5x1ml
Description
Description
Storage:
2 years at -20℃.
Code
P3082
Size
5x1ml
Contents: 2x TM TMHS Reaction Mix, HS Taq DNA Polymerase (2.5U/ l)
TMHS Kit -high specificity
Pfu Kit -high fidelity
Storage:
2 years at -20℃.
Code
P3022
Size
5x1ml
Description
Pfu Kit contains a Pfu DNA Polymerase, and a premixed, ready-to-use 2xPfu
Reaction Mix to perform 200 PCR reactions.
The PCR Kit contains a recombinant, high purified Taq DNA Polymerase, and
a premixed, ready-to-use 2xPCR Reaction Mix to perform 200 PCR reactions.
TM TM HS HS Kit contains a Taq DNA Polymerase, and a premixed, ready-to-use
2x Reaction Mix to perform 200 PCR reaction. TMHS
2+2xPfu Reaction Mix is a premixed solution containing dNTPs, Mg and Pfu
buffer at optional concentrations for efficient PCR amplification. PCR amplifi-
cation sensitivity is controllable because the amount of Pfu DNA Polymeras
Contents: 2xPCR Reaction Mix, Taq DNA Polymerase(2.5U/ l)
2+2xPCR Reaction Mix is a premixed solution containing dNTPs, Mg and PCR
buffer at optional concentrations for efficient PCR amplification. PCR amplifi-
cation sensitivity is controllable because the amount of Taq DNA Polymeras
is flexible.
2+2x Reaction Mix is a premixed solution containing dNTPs, Mg and
buffer at optional concentrations for efficient PCR amplification. PCR
amplification sensitivity is controllable because the amount of Taq DNA
TMHSTMHS
TM HS
Contents: 2xPfu Reaction Mix, Pfu DNA Polymerase(2.5U/ l)
PCR, qPCR & RT-PCR
E-mail: [email protected] Fax: +86-20-87791381
PC
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-PC
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Polymerase is flexible.
is flexible.
prepared sample
Code
P2071a
P2072a
Size
1ml
5x1ml
Description
TMFS Mix Direct for Blood (2x)
Storage:
2 years at -20℃.
Contents: 2x Mix B, Nuclease-free WaterTMFS
TMFSTM FS
Mix Direct for Blood (2x) is a premixed, ready-to-use solution
containing Taq DNA Polymerase, dNTPs, and all other PCR
components, except DNA template and primers. This mix is specific
for whole blood amplification. It contribute to fast, specific, sensitive
and reproducible PCR by reducing the fisk of contributes to highly
reproducible PCR by reducing the risk of pipetting errors, miscalc-
ulation and contamination. This product cut off the process of com-
TMFS Mix Direct for Tissue (2x)
Contents: 2x Mix T, Extraction Solution, Neutralization Solution
Nuclease-free Water
TMFS
whole or diluted blood
2x Mix B TMFS
primers water
plicated and waste time DNA extraction.
Code
P2071b
P2072b
Size
1ml
5x1ml
Description
Storage:
2 years at -20℃.
TMFSTM FS
Mix Direct for Tissue (2x) is a premixed, ready-to-use solution
containing Taq DNA Polymerase, dNTPs, and all other PCR
components, except DNA template and primers. This mix is specific
for tissue direct amplification. It contribute to fast, specific, sensitive
and reproducible PCR by reducing the fisk of contributes to highly
reproducible PCR by reducing the risk of pipetting errors, miscalc-
ulation and contamination. This product cut off the process of com-
plicated and waste time DNA extraction.
Sample preparation
2x Mix T TMFS
primers water
http://dongshengbio.com Tel: +86-20-87791356
PC
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PC
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RT
-PC
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PCR Kit -routine PCR
Storage:
2 years at -20℃.
Code
P3012
Size
5x1ml
Description
Description
Storage:
2 years at -20℃.
Code
P3082
Size
5x1ml
Contents: 2x TM TMHS Reaction Mix, HS Taq DNA Polymerase (2.5U/ l)
TMHS Kit -high specificity
Pfu Kit -high fidelity
Storage:
2 years at -20℃.
Code
P3022
Size
5x1ml
Description
Pfu Kit contains a Pfu DNA Polymerase, and a premixed, ready-to-use 2xPfu
Reaction Mix to perform 200 PCR reactions.
The PCR Kit contains a recombinant, high purified Taq DNA Polymerase, and
a premixed, ready-to-use 2xPCR Reaction Mix to perform 200 PCR reactions.
TM TM HS HS Kit contains a Taq DNA Polymerase, and a premixed, ready-to-use
2x Reaction Mix to perform 200 PCR reaction. TMHS
2+2xPfu Reaction Mix is a premixed solution containing dNTPs, Mg and Pfu
buffer at optional concentrations for efficient PCR amplification. PCR amplifi-
cation sensitivity is controllable because the amount of Pfu DNA Polymeras
Contents: 2xPCR Reaction Mix, Taq DNA Polymerase(2.5U/ l)
2+2xPCR Reaction Mix is a premixed solution containing dNTPs, Mg and PCR
buffer at optional concentrations for efficient PCR amplification. PCR amplifi-
cation sensitivity is controllable because the amount of Taq DNA Polymeras
is flexible.
2+2x Reaction Mix is a premixed solution containing dNTPs, Mg and
buffer at optional concentrations for efficient PCR amplification. PCR
amplification sensitivity is controllable because the amount of Taq DNA
TMHSTMHS
TM HS
Contents: 2xPfu Reaction Mix, Pfu DNA Polymerase(2.5U/ l)
PCR, qPCR & RT-PCR
E-mail: [email protected] Fax: +86-20-87791381
PC
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30
Polymerase is flexible.
is flexible.
Storage:
2 years at -20℃.
Code
P3062
Size
5x1ml
Description
Description
Storage:
2 years at -20℃.
Code
P3072
Size
5x1ml
Contents: 2x TM TMFS Reaction Mix, FS Taq DNA Polymerase (2.5U/ l)
TMFS Kit -fast PCR
dNTPs
Storage:
2 years at -20℃.
Code
P9011
P9012
P9013
P9014
P9071
P9081
P9091
P9101
R2051
Size
1ml
0.5ml
1ml
4x0.25ml
0.5ml
0.5ml
0.5ml
0.5ml
0.5ml
Description
Long Taq Kit contains a Long Taq DNA Polymerase, PCR Enhancer, and a
premixed, ready-to-use 2xPCR Reaction Mix to perform 200 PCR reactions.
TM TM FS FS Kit contains a Taq DNA Polymerase, and a premixed, ready-to-use
2x Reaction Mix to perform 200 PCR reaction. TMFS
Contents: 2xLong Reaction Mix, PCR Enhancer, Taq DNA Polymerase(2.5U/ l)
2+2xLong Reaction Mix is a premixed solution containing dNTPs, Mg and Long
PCR buffer at optional concentrations for efficient PCR amplification. PCR
amplification sensitivity is controllable because the amount of Lont Taq DNA
.
2+2x Reaction Mix is a premixed solution containing dNTPs, Mg and
buffer at optional concentrations for efficient PCR amplification. PCR
amplification sensitivity is controllable because the amount of Taq DNA
TMFSTMFS
TM FS
Long Taq Kit -for complex templates
Product Name
dNTP Mix (2.5mM)
dNTP Mix (10mM)
dNTP Mix (10mM)
dNTP Set (100mM)
dATP (100mM)
dTTP (100mM)
dCTP (100mM)
dGTP (100mM)
dNTPs (10mM, RNase-free)
dNTP Mix is a aqueous solutions at PH 7.0
containing dATP, dTTP, dCTP, dGTP each
at a final concentration of 2.5mM (or 10mM).
http://dongshengbio.com Tel: +86-20-87791356
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Polymerase is flexible.
Polymeras is flexible
dNTPs (10mM, RNase-free) is ideal for RT-
PCR reaction.
PCR Buffer
Storage:
2 years at -20℃.
Code
P5011
P5011a
P5011b
P9031
Size
4x1.25ml
4x1.25ml
6x1.25ml
4x1.25ml
Description Product Name2+10xPCR Buffer (with 15mM Mg )
2+10xPCR Buffer (without Mg )
10xPCR Buffer2+ (with Mg concentration set)
25mM MgCl2
10xPCR Buffer is recommended
for all PCR applications with Taq
DNA Polymerase (both recombinant
Nuclease-free Water
Storage:
2 years at -20℃.
Code
P9021
P9022
P9023
Size
5x1ml
100ml
500ml
Description Product Name
Nuclease-free WaterNuclease-free Water is deionized and
0.22 m membrane-filtered. It is suitable
for all molecular biology applications.
PCR Enhancer
Storage:
2 years at -20℃.
Code
P9041
Size
0.5ml
Description Product Name
PCR EnhancerPCR Enhancer is used in conjunction with
DNA polymerase to optimized PCR of pro-
blemetic and/or GC-rich templates. The
system offers higher specificity, broader
magnesium concentration, broader anneal-
ing temperature and improved thermostable
PCR Sample Preparation Solution
Storage:
2 years at room temperature
Code
P9051
P9052
Size
50 pres
200 preps
Description Product Name
PCR Sample Preparation SolutionPCR Sample Preparation Solution is widely
used for sample preparation before PCR
reaction. It can be used for samples like
tissue, hair, buccal swab, culture cells,
Contents: Extraction Solution
Neutralization Solution
PCR, qPCR & RT-PCR
E-mail: [email protected] Fax: +86-20-87791381
PC
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serum, DNA virus.
DNA polymerase.
or native)
Storage:
2 years at -20℃.
Code
P3062
Size
5x1ml
Description
Description
Storage:
2 years at -20℃.
Code
P3072
Size
5x1ml
Contents: 2x TM TMFS Reaction Mix, FS Taq DNA Polymerase (2.5U/ l)
TMFS Kit -fast PCR
dNTPs
Storage:
2 years at -20℃.
Code
P9011
P9012
P9013
P9014
P9071
P9081
P9091
P9101
R2051
Size
1ml
0.5ml
1ml
4x0.25ml
0.5ml
0.5ml
0.5ml
0.5ml
0.5ml
Description
Long Taq Kit contains a Long Taq DNA Polymerase, PCR Enhancer, and a
premixed, ready-to-use 2xPCR Reaction Mix to perform 200 PCR reactions.
TM TM FS FS Kit contains a Taq DNA Polymerase, and a premixed, ready-to-use
2x Reaction Mix to perform 200 PCR reaction. TMFS
Contents: 2xLong Reaction Mix, PCR Enhancer, Taq DNA Polymerase(2.5U/ l)
2+2xLong Reaction Mix is a premixed solution containing dNTPs, Mg and Long
PCR buffer at optional concentrations for efficient PCR amplification. PCR
amplification sensitivity is controllable because the amount of Lont Taq DNA
.
2+2x Reaction Mix is a premixed solution containing dNTPs, Mg and
buffer at optional concentrations for efficient PCR amplification. PCR
amplification sensitivity is controllable because the amount of Taq DNA
TMFSTMFS
TM FS
Long Taq Kit -for complex templates
Product Name
dNTP Mix (2.5mM)
dNTP Mix (10mM)
dNTP Mix (10mM)
dNTP Set (100mM)
dATP (100mM)
dTTP (100mM)
dCTP (100mM)
dGTP (100mM)
dNTPs (10mM, RNase-free)
dNTP Mix is a aqueous solutions at PH 7.0
containing dATP, dTTP, dCTP, dGTP each
at a final concentration of 2.5mM (or 10mM).
http://dongshengbio.com Tel: +86-20-87791356
PC
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-PC
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Polymerase is flexible.
Polymeras is flexible
dNTPs (10mM, RNase-free) is ideal for RT-
PCR reaction.
PCR Buffer
Storage:
2 years at -20℃.
Code
P5011
P5011a
P5011b
P9031
Size
4x1.25ml
4x1.25ml
6x1.25ml
4x1.25ml
Description Product Name2+10xPCR Buffer (with 15mM Mg )
2+10xPCR Buffer (without Mg )
10xPCR Buffer2+ (with Mg concentration set)
25mM MgCl2
10xPCR Buffer is recommended
for all PCR applications with Taq
DNA Polymerase (both recombinant
Nuclease-free Water
Storage:
2 years at -20℃.
Code
P9021
P9022
P9023
Size
5x1ml
100ml
500ml
Description Product Name
Nuclease-free WaterNuclease-free Water is deionized and
0.22 m membrane-filtered. It is suitable
for all molecular biology applications.
PCR Enhancer
Storage:
2 years at -20℃.
Code
P9041
Size
0.5ml
Description Product Name
PCR EnhancerPCR Enhancer is used in conjunction with
DNA polymerase to optimized PCR of pro-
blemetic and/or GC-rich templates. The
system offers higher specificity, broader
magnesium concentration, broader anneal-
ing temperature and improved thermostable
PCR Sample Preparation Solution
Storage:
2 years at room temperature
Code
P9051
P9052
Size
50 pres
200 preps
Description Product Name
PCR Sample Preparation SolutionPCR Sample Preparation Solution is widely
used for sample preparation before PCR
reaction. It can be used for samples like
tissue, hair, buccal swab, culture cells,
Contents: Extraction Solution
Neutralization Solution
PCR, qPCR & RT-PCR
E-mail: [email protected] Fax: +86-20-87791381
PC
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serum, DNA virus.
DNA polymerase.
or native)
Real Time qPCR Master Mix
Keep Features:
·High Specificity
·High Sensitivity
·Universal
eliminate non-specific and formation of primer dimers.
detects low copy number targets.
suitable for most real-time thermal cyclers.
Plasmid DNA 10ng, 1ng, 0.1ng, 0.01ng, 0.001, 0.0001ng/ul in BIO-RAD CFX96
DongshengDongsheng
Plasmid DNA 1ng, 0.1ng, 0.01ng, 0.001ng/ul in BIO-RAD CFX96
Company ACompany A
SYBR Green qPCR Mix
SYBR Green qPCR Mix (High Rox+)
SYBR Green qPCR Mix (Low Rox+)
Probe qPCR Mix
Probe qPCR Mix (High Rox+)
Probe qPCR Mix (Low Rox+)
Product
Machine
Instrument guide table
Rox Reference Dye
http://dongshengbio.com Tel: +86-20-87791356
PC
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SYBR Green qPCR Mix -realtime PCR
Storage:
Protect from light, 2 years at -20℃.
Code
P2091
P2092
Size
1ml
5x1ml
Description
Contents: 2xSYBR Green qPCR Mix , Nuclease-free Water
SYBR Green qPCR Mix (2x) is designed for high-performance, high-through-
put, real-time PCR. The kit contains a Hot Start Taq DNA Polymerase engineered
a process of molecular evolution. This results in a unique enzyme, specifically
designed for qPCR using SYBR Green I dye chemistry.
SYBR Green qPCR Mix (2x) is a convenient premix of the components (except
primers, template, and water) necessary to perform real-time polymerase chain
reaction (PCR) using SYBR Green I dye with enhanced sensitivity and specificity.
The SYBR Green dye binds to double-strand (ds) DNA, thus providing a fluore-
sent signal that reflects the amount of dsDNA product generated during PCR.
Amplification Melt Curve
Above are with Dongsheng’s SYBR Green qPCR Mix in STRATAGENE Mx3005.
Sample: HBV Serum, Objective band: 210bp.
Ampification condition: 95C 20s in the first cycle, 95C 10s, 55C 20s and 72C 30s in the less 40 cycles.
Recommended PCR
Reaction ( 20µl
reaction system)
Components
2xSYBR Green qPCR Mix
Forward primer (10µM)
Reverse primer (10 µM)
Template DNA
Nuclease-free, Water
Volume
10 µl
0.4 µl
0.4 µl
variable
to 20 µl
Final concentration
1X
0.2 µM
0.2 µM
<100ng
-
Recommend PCR
Cycling System
Initial Denaturation
Denature
Anneal
Extend*
Melting Curve Analysis
94℃
95℃
60℃
72℃
3 min
15 sec
15 sec
20 sec
40 cycles
*Dongsheng cat #P9021/P9022 work better for 3 step cycling protocol. We don’t
recommend to use 2 step cycling protocol with this product.
PCR, qPCR & RT-PCR
E-mail: [email protected] Fax: +86-20-87791381
PC
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-PC
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®
®
®
®
®
®
®
®
Real Time qPCR Master Mix
Keep Features:
·High Specificity
·High Sensitivity
·Universal
eliminate non-specific and formation of primer dimers.
detects low copy number targets.
suitable for most real-time thermal cyclers.
Plasmid DNA 10ng, 1ng, 0.1ng, 0.01ng, 0.001, 0.0001ng/ul in BIO-RAD CFX96
DongshengDongsheng
Plasmid DNA 1ng, 0.1ng, 0.01ng, 0.001ng/ul in BIO-RAD CFX96
Company ACompany A
SYBR Green qPCR Mix
SYBR Green qPCR Mix (High Rox+)
SYBR Green qPCR Mix (Low Rox+)
Probe qPCR Mix
Probe qPCR Mix (High Rox+)
Probe qPCR Mix (Low Rox+)
Product
Machine
Instrument guide table
Rox Reference Dye
http://dongshengbio.com Tel: +86-20-87791356
PC
R, q
PC
R &
RT
-PC
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33
SYBR Green qPCR Mix -realtime PCR
Storage:
Protect from light, 2 years at -20℃.
Code
P2091
P2092
Size
1ml
5x1ml
Description
Contents: 2xSYBR Green qPCR Mix , Nuclease-free Water
SYBR Green qPCR Mix (2x) is designed for high-performance, high-through-
put, real-time PCR. The kit contains a Hot Start Taq DNA Polymerase engineered
a process of molecular evolution. This results in a unique enzyme, specifically
designed for qPCR using SYBR Green I dye chemistry.
SYBR Green qPCR Mix (2x) is a convenient premix of the components (except
primers, template, and water) necessary to perform real-time polymerase chain
reaction (PCR) using SYBR Green I dye with enhanced sensitivity and specificity.
The SYBR Green dye binds to double-strand (ds) DNA, thus providing a fluore-
sent signal that reflects the amount of dsDNA product generated during PCR.
Amplification Melt Curve
Above are with Dongsheng’s SYBR Green qPCR Mix in STRATAGENE Mx3005.
Sample: HBV Serum, Objective band: 210bp.
Ampification condition: 95C 20s in the first cycle, 95C 10s, 55C 20s and 72C 30s in the less 40 cycles.
Recommended PCR
Reaction ( 20µl
reaction system)
Components
2xSYBR Green qPCR Mix
Forward primer (10µM)
Reverse primer (10 µM)
Template DNA
Nuclease-free, Water
Volume
10 µl
0.4 µl
0.4 µl
variable
to 20 µl
Final concentration
1X
0.2 µM
0.2 µM
<100ng
-
Recommend PCR
Cycling System
Initial Denaturation
Denature
Anneal
Extend*
Melting Curve Analysis
94℃
95℃
60℃
72℃
3 min
15 sec
15 sec
20 sec
40 cycles
*Dongsheng cat #P9021/P9022 work better for 3 step cycling protocol. We don’t
recommend to use 2 step cycling protocol with this product.
PCR, qPCR & RT-PCR
E-mail: [email protected] Fax: +86-20-87791381
PC
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RT
-PC
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®
®
®
®
®
®
®
®
Melt CurveAmplification
Above are with Dongsheng’s SYBR Green qPCR Mix (High Rox+) in STRATAGENE Mx3005
+SYBR Green qPCR Mix (Rox ) -high accurate real-time PCR
Storage:
Protect from light, 2 years at -20℃.
Code
P2091a
P2092a
P2091b
P2092b
Size
1ml
5x1ml
1ml
5x1ml
Description
Contents:
#P2091a/P2092a: 2xSYBR Green qPCR Mix (Low Rox ) , Nuclease-free Water+
SYBR Green qPCR Mix (Rox ) (2x) is designed for high-performance, high-
throughput, real-time PCR. The kit contains a Hot Start Taq DNA Polymerase
engineered a process of molecular evolution. This results in a unique enzyme,
specifically designed for qPCR using SYBR Green I dye chemistry. It also
contains rox reference dye, which is ideal for using in amplification and detec-
tion of DNA in qPCR on real-time instruments (e.g. ABI) that supports normali-
zation with rox reference dye .
+
Recommended PCR
Reaction ( 20µl
reaction system)
Components
2xSYBR Green qPCR Mix (Rox )
Forward primer (10µM)
Reverse primer (10 µM)
Template DNA
Nuclease-free, Water
+
Volume
10 µl
0.4 µl
0.4 µl
variable
to 20 µl
Final concentration
1X
0.2 µM
0.2 µM
<100ng
-
Recommend PCR
Cycling System
Initial Denaturation
Denature
Anneal
Extend*
Melting Curve Analysis
94℃
95℃
60℃
72℃
3 min
15 sec
15 sec
20 sec
40 cycles
*Dongsheng cat #P9021a/P9022a/P9021b/P9022b work better for 3 step cycling
protocol. We don’t recommend to use 2 step cycling protocol with this product.
#P2091b/P2092b: 2xSYBR Green qPCR Mix (High Rox ) , Nuclease-free Water+
http://dongshengbio.com Tel: +86-20-87791356
PC
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®
®
®
®
®
®
M-MLV (recombinant) Cat.# R1041-5000U, R1042-10000U
Storage:
2 years at -20℃.
R1041
25 µl
100 µl
R1042
50 µl
200 µl
Description
Contents
M-MLV (200U/ µl)
5xfirst-strand buffer (5x)
Moloney Murine Leukemia Virus Reverse Trans-
criptase (M-MLV RT) uses single-stranded RNA
or DNA in the presence of a primer to synthesize
a complementary DNA strand. This enzyme is
isolated from E. coli expressing a portion of the
pol gene of M-MLV on a plasmid. The enzyme is
used to synthesize first-strand cDNA up to 5kb.
RT-PCR Kit Cat.# R1011-20 rxns, R1012-100 rxns
Storage:
2 years at -20℃.
R1011
20µl
12µl
20µl
20µl
80µl
1ml
50µl
R1011
100µl
60µl
100µl
100µl
400µl
5x1ml
250µl
Description
Contents
M-MLV (200U/ µl)
RNasin (40U/µl)
(50µM)
Random primer (50µM)
5xfirst-strand buffer (5x)
RNase-free ddH2O
dNTPs (10mM each)
Oligo d(T) 15
RT-PCR Kit is optimized to synthesize first-strand
cDNA from purif ied poly (A)+ or total RNA.
The kit Reverse Transcriptase is a version of M-MLV
RT that has been engineered to reduce RNase H
activity and provide increased thermal stability. The
enzyme is used to synthsize cDNA at a temperature
range of 42-55℃ , providing increased specificity,
higher yields of cDNA, and more full-length product
R1041can be used for 25 reactions, R1042 can
be used for 50 reactions (20 µl PCR system).
Rnasin (RNase inhibitor) Cat.# R2011-1000U
Storage:
2 years at -20℃.
R2011
25µl
Description
Contents
RNasin (40U/µl)
Rnase is a ribonuclease inhibitor purified form
human placenta. RNase recuces the activity of
RNase A-type enzymes in a variety of organisms.
It is free of endodeoxyribonucleases, exodeoxy-
ribonucleases, ribonucleases and phosphatases.
PCR, qPCR & RT-PCR
E-mail: [email protected] Fax: +86-20-87791381
PC
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-PC
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Reverse Transcriptase
than other reverse transcriptased.
Melt CurveAmplification
Above are with Dongsheng’s SYBR Green qPCR Mix (High Rox+) in STRATAGENE Mx3005
+SYBR Green qPCR Mix (Rox ) -high accurate real-time PCR
Storage:
Protect from light, 2 years at -20℃.
Code
P2091a
P2092a
P2091b
P2092b
Size
1ml
5x1ml
1ml
5x1ml
Description
Contents:
#P2091a/P2092a: 2xSYBR Green qPCR Mix (Low Rox ) , Nuclease-free Water+
SYBR Green qPCR Mix (Rox ) (2x) is designed for high-performance, high-
throughput, real-time PCR. The kit contains a Hot Start Taq DNA Polymerase
engineered a process of molecular evolution. This results in a unique enzyme,
specifically designed for qPCR using SYBR Green I dye chemistry. It also
contains rox reference dye, which is ideal for using in amplification and detec-
tion of DNA in qPCR on real-time instruments (e.g. ABI) that supports normali-
zation with rox reference dye .
+
Recommended PCR
Reaction ( 20µl
reaction system)
Components
2xSYBR Green qPCR Mix (Rox )
Forward primer (10µM)
Reverse primer (10 µM)
Template DNA
Nuclease-free, Water
+
Volume
10 µl
0.4 µl
0.4 µl
variable
to 20 µl
Final concentration
1X
0.2 µM
0.2 µM
<100ng
-
Recommend PCR
Cycling System
Initial Denaturation
Denature
Anneal
Extend*
Melting Curve Analysis
94℃
95℃
60℃
72℃
3 min
15 sec
15 sec
20 sec
40 cycles
*Dongsheng cat #P9021a/P9022a/P9021b/P9022b work better for 3 step cycling
protocol. We don’t recommend to use 2 step cycling protocol with this product.
#P2091b/P2092b: 2xSYBR Green qPCR Mix (High Rox ) , Nuclease-free Water+
http://dongshengbio.com Tel: +86-20-87791356
PC
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-PC
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®
®
®
®
®
®
M-MLV (recombinant) Cat.# R1041-5000U, R1042-10000U
Storage:
2 years at -20℃.
R1041
25 µl
100 µl
R1042
50 µl
200 µl
Description
Contents
M-MLV (200U/ µl)
5xfirst-strand buffer (5x)
Moloney Murine Leukemia Virus Reverse Trans-
criptase (M-MLV RT) uses single-stranded RNA
or DNA in the presence of a primer to synthesize
a complementary DNA strand. This enzyme is
isolated from E. coli expressing a portion of the
pol gene of M-MLV on a plasmid. The enzyme is
used to synthesize first-strand cDNA up to 5kb.
RT-PCR Kit Cat.# R1011-20 rxns, R1012-100 rxns
Storage:
2 years at -20℃.
R1011
20µl
12µl
20µl
20µl
80µl
1ml
50µl
R1011
100µl
60µl
100µl
100µl
400µl
5x1ml
250µl
Description
Contents
M-MLV (200U/ µl)
RNasin (40U/µl)
(50µM)
Random primer (50µM)
5xfirst-strand buffer (5x)
RNase-free ddH2O
dNTPs (10mM each)
Oligo d(T) 15
RT-PCR Kit is optimized to synthesize first-strand
cDNA from purif ied poly (A)+ or total RNA.
The kit Reverse Transcriptase is a version of M-MLV
RT that has been engineered to reduce RNase H
activity and provide increased thermal stability. The
enzyme is used to synthsize cDNA at a temperature
range of 42-55℃ , providing increased specificity,
higher yields of cDNA, and more full-length product
R1041can be used for 25 reactions, R1042 can
be used for 50 reactions (20 µl PCR system).
Rnasin (RNase inhibitor) Cat.# R2011-1000U
Storage:
2 years at -20℃.
R2011
25µl
Description
Contents
RNasin (40U/µl)
Rnase is a ribonuclease inhibitor purified form
human placenta. RNase recuces the activity of
RNase A-type enzymes in a variety of organisms.
It is free of endodeoxyribonucleases, exodeoxy-
ribonucleases, ribonucleases and phosphatases.
PCR, qPCR & RT-PCR
E-mail: [email protected] Fax: +86-20-87791381
PC
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-PC
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Reverse Transcriptase
than other reverse transcriptased.
Oligo d(T) Primer Cat.# R2021-20µl 15
Storage:
2 years at -20℃.
R2021
20 µl
Description
Contents
(50µM)Oligo d(T) Primer 15
Oligo d(T) Primer is a synthetic single-stranded 15-15
mer oligobucleotide with 5’-and 3’-hydroxyl ends. This
primer is supplied as an aqueous solution and can be
Random Primer Cat.# R2031-20µl (6mer)
DEPC-treated Water Cat.# R2041-30ml, R2042-100ml
Storage:
2 years at 4℃.
R2041
30ml
Description
Contents
DEPC-treated Water
DEPC-treated Water is deionized, diethylpyrocarbonate
(DEPC) treated and 0.22 µM membrane-filtered. It is
recomme nded to be used in any application where RNA
Storage:
2 years at -20℃.
R2031
20 µl
Description
Contents
(50µM)Random Primer (6mer)
Random Primer is a mixture of single-stranded 6-(6mer)
mer random hexanucleotides with 5’-and 3’-hydroxyl
ends. This primer is supplied as an aqueous solution
R2042
100ml
RNA Stabilization Solution Cat.# R2071-20ml, R2072-100ml
Storage:
2 years at room temperature.
R2071
20ml
Description
Contents
RNA Stabilization Solution
RNA Stabi lization Sol ut ion is an aqu eou s tissue storage
reag ent that stabi lize and protects ce llular RNA in intact,
unf rozen tissue samples. It el iminat es the nee d to imm-
edi ately process tissue samples or to freez e samples in
R2072
100ml
used for first strand cDNA synthesis.
and can be used for first strand cDNA synthesis.
is involed.
liquid nitrogen for later processing.
http://dongshengbio.com Tel: +86-20-87791356
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2
3. DNA Purification Kits
Oligo d(T) Primer Cat.# R2021-20µl 15
Storage:
2 years at -20℃.
R2021
20 µl
Description
Contents
(50µM)Oligo d(T) Primer 15
Oligo d(T) Primer is a synthetic single-stranded 15-15
mer oligobucleotide with 5’-and 3’-hydroxyl ends. This
primer is supplied as an aqueous solution and can be
Random Primer Cat.# R2031-20µl (6mer)
DEPC-treated Water Cat.# R2041-30ml, R2042-100ml
Storage:
2 years at 4℃.
R2041
30ml
Description
Contents
DEPC-treated Water
DEPC-treated Water is deionized, diethylpyrocarbonate
(DEPC) treated and 0.22 µM membrane-filtered. It is
recomme nded to be used in any application where RNA
Storage:
2 years at -20℃.
R2031
20 µl
Description
Contents
(50µM)Random Primer (6mer)
Random Primer is a mixture of single-stranded 6-(6mer)
mer random hexanucleotides with 5’-and 3’-hydroxyl
ends. This primer is supplied as an aqueous solution
R2042
100ml
RNA Stabilization Solution Cat.# R2071-20ml, R2072-100ml
Storage:
2 years at room temperature.
R2071
20ml
Description
Contents
RNA Stabilization Solution
RNA Stabi lization Sol ut ion is an aqu eou s tissue storage
reag ent that stabi lize and protects ce llular RNA in intact,
unf rozen tissue samples. It el iminat es the nee d to imm-
edi ately process tissue samples or to freez e samples in
R2072
100ml
used for first strand cDNA synthesis.
and can be used for first strand cDNA synthesis.
is involed.
liquid nitrogen for later processing.
http://dongshengbio.com Tel: +86-20-87791356
PC
R, q
PC
R &
RT
-PC
R
2
375
2
3. DNA Purification Kits
http://dongshengbio.com Tel: +86-20-87791356
DNA Purification Kits
Dongsheng Biotech offers a broad range of kits for the isolation and
purification of genomic and plasmid DNA from a wide variety of samples,
including tissue, cells , blood, serum, bacterial, yeast, and plants samples .
We provide three series for different materials purification:'
Plasmid DNA Extraction Kits
DNA Product Purification Kits
Genimic DNA Purification Kits
DN
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ati
on
Kit
s
3
39
DNA Purification Kits
E-mail: [email protected] Fax: +86-20-87791381
Guides of DNA Purification Kits
Quick Genomic DNA Extraction
Kit
Standard
Agarose Gel Safe, Healthy
Gel Extraction Kit
Eco-Friendly Gel Extraction Kit
PCR Product/
DNA Fragment
SolutionSafe, Healthy
PCR and DNA Fragment
Purification Kit
Eco-Friendly PCR and DNA
Fragment Purification Kit
DNA Product
Purification
Genomic DNA
Purification
Insect/ Animal
Nematoda
Sample size: less than
10mg tissue
Blood
Sample size: 0.l-1ml
flesh blood/ frozen blood
Blood Genomic DNA
Extraction Kit
Sample size: less than
200ul whole bloodQuick Blood Genomic DNA
Extraction Kit
Tissue/
Cell
Bacteria
Yeast
Sample size: less than6 710mg tissue/ 10 -10
culture cell
Sample size: 0.5-2ml/6less than 10 -10 bacteria
Sample size: less than71-5x10 Yeast
Quick Tissue/Culture Cells
Genomic DNA Extraction Kit
Quick Bacteria Genomic
DNA Extraction Kit
Quick Yeast Genomic DNA
Extraction Kit
Application Material Experimental requirement Product Choose
Plasmid DNA
ExtractionBacteria
Sample size:1-5ml
Sample size:1-5ml Safe, Healthy
High Purity Plasmid Miniprep Kit
Sample size:100-200ml
Simple, Convenient
Eco-Friendly Plasmid Miniprep Kit
Plasmid DNA Maxiprep Kit (High
Purifity, Silica-membrant Spin
Column)
Sample size:100-200ml Plasmid DNA Maxiprep Kit (High
Purifity, Silica-precipitation)
Sample size:100-200ml
Endotoxin-Free
Plasmid DNA Maxiprep Kit
(Endotoxin-Free)
Yeast Sample size:1-5ml Yeast Plasmid DNA Miniprep Kit
DN
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fic
ati
on
Kit
s
3
40
http://dongshengbio.com Tel: +86-20-87791356
DNA Purification Kits
Dongsheng Biotech offers a broad range of kits for the isolation and
purification of genomic and plasmid DNA from a wide variety of samples,
including tissue, cells , blood, serum, bacterial, yeast, and plants samples .
We provide three series for different materials purification:'
Plasmid DNA Extraction Kits
DNA Product Purification Kits
Genimic DNA Purification Kits
DN
A P
uri
fic
ati
on
Kit
s
3
39
DNA Purification Kits
E-mail: [email protected] Fax: +86-20-87791381
Guides of DNA Purification Kits
Quick Genomic DNA Extraction
Kit
Standard
Agarose Gel Safe, Healthy
Gel Extraction Kit
Eco-Friendly Gel Extraction Kit
PCR Product/
DNA Fragment
SolutionSafe, Healthy
PCR and DNA Fragment
Purification Kit
Eco-Friendly PCR and DNA
Fragment Purification Kit
DNA Product
Purification
Genomic DNA
Purification
Insect/ Animal
Nematoda
Sample size: less than
10mg tissue
Blood
Sample size: 0.l-1ml
flesh blood/ frozen blood
Blood Genomic DNA
Extraction Kit
Sample size: less than
200ul whole bloodQuick Blood Genomic DNA
Extraction Kit
Tissue/
Cell
Bacteria
Yeast
Sample size: less than6 710mg tissue/ 10 -10
culture cell
Sample size: 0.5-2ml/6less than 10 -10 bacteria
Sample size: less than71-5x10 Yeast
Quick Tissue/Culture Cells
Genomic DNA Extraction Kit
Quick Bacteria Genomic
DNA Extraction Kit
Quick Yeast Genomic DNA
Extraction Kit
Application Material Experimental requirement Product Choose
Plasmid DNA
ExtractionBacteria
Sample size:1-5ml
Sample size:1-5ml Safe, Healthy
High Purity Plasmid Miniprep Kit
Sample size:100-200ml
Simple, Convenient
Eco-Friendly Plasmid Miniprep Kit
Plasmid DNA Maxiprep Kit (High
Purifity, Silica-membrant Spin
Column)
Sample size:100-200ml Plasmid DNA Maxiprep Kit (High
Purifity, Silica-precipitation)
Sample size:100-200ml
Endotoxin-Free
Plasmid DNA Maxiprep Kit
(Endotoxin-Free)
Yeast Sample size:1-5ml Yeast Plasmid DNA Miniprep Kit
DN
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Kit
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Plasmid Extraction Series
Genomic DNA Purification Serious
Quick Genomic DNA Extraction Kit
Blood Genomic DNA Extraction Kit
Quick Blood Genomic DNA Extraction Kit
Quick Tissue/Culture Cells Genomic DNA Extraction Kit
Quick Bacteria Genomic DNA Extraction Kit
Quick Yeast Genomic DNA Extraction Kit
Marine Animal Tissue Genomic DNA Extraction Kit
Product Name Cat # Size
N1111
N1112
N1121
N1122
N1131
N1132
N1141
N1142
N1151
N1152
N1161
N1162
N1171
N1172
50 preps
100 preps
50 preps
100 preps
50 preps
100 preps
50 preps
100 preps
50 preps
100 preps
50 preps
100 preps
50 preps
100 preps
http://dongshengbio.com Tel: +86-20-8779135641
Product Name Cat # Size
High Purity Plasmid Miniprep kit
Eco-Friendly Plasmid Miniprep Kit
Plasmid DNA Maxiprep Kit (High Purity, Silica-mimbrane
Spin Column)
Plasmid DNA Maxiprep Kit (High Purity, Silica preciptation)
Plasmid DNA Maxiprep Kit (Endotoxin-free)
Yeast Plasmid DNA Miniprep Kit
N1011
N1012
N1021
N1022
N1031
N1041
N1051
N1061
N1062
50 preps
100 preps
50 preps
100 preps
10 preps
10 preps
10 preps
50 preps
100 preps
PCP Product Purification Series
Gel Extraction Kit
Eco-Feiendly Gel Extraction Kit
PCR and DNA Fragment Purification Kit
Product Name Cat # Size
N1071
N1072
N1081
N1082
N1091
N1092
50 preps
100 preps
50 preps
100 preps
50 preps
100 preps
3
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Kit
s
DNA Purification Kits
Related Products
Product Name Cat # Size
Proteinase K (20mg/ml)
Proteinase K
(Lyophilized powder)
RNase A (20mg/ml)
RNase A (100mg/ml)
RNase A
(Lyophilized powder)
Dnase I
N9011
N9016
N9017
N9041
N9042
N9046
N9047
N9066
1ml
100mg
1g
1ml
1ml
100mg
1g
1g
High Purity Plasmid Miniprep Kit
Resuspend CellsLyse andNeutralize
Bind DNA
Wash column
Elute pure DNA
Description
High Purity Plasmid Miniprep Kit is designed
for rapid and cost-effective small-scale pre-
paration of high quality plasmid DNA from
recombinant E. Coli cultures. The kit utilizes
an exclusive silicabased membrane techno-
logy in the form of a convenient spin column.
Each spin column can recover up to 40ug of
plasmid DNA. The kit can be used for any size
plasmid and cosmids purification efficiently.
Content
Size
RNase A
Resuspension Solution
Lysis Solution
Neutralization Solution
Wash Buffer PB
Wash Buffer W
Elution Buffer
(10mM Tris-HCl, pH 8.5)
Mini Spin Columns
N1011
50preps
150
15ml
15ml
20ml
30ml
30ml
5ml
50each
µl
For the initial use of the kit, dilute the Wash
Buffer W with ethanol 1:1.5 (v/v).
Figure .A 5 kb PCR fragment was cloned into pCMV vector. Lane M: 1kb ladderLane 1and 2 : Plasmid DNA from overnight bacterial cultures of recombinant clones was purified using the Plasmid Miniprep Kit (N1011). Lane 3 and 4 : analyzed by double digestion with EcoRI and HindIII in 30 min at 37℃.
M 1 2 3 4 M
Feature
Fast-procedure takes only 30 min
Efficient-can get 10-40 g high copy plas-
mid DNA in one prep
High purity-purified DNA is ready for down-
stream applications
µ
Application
Fast isolat ion of high purity plasmid DNA are
ready for di rect use in al l appl icat ions, inc lud ing:
Res triction digestion
DNA Seq uencing
In vitro transcript ion
Store RNase at -20℃.
Other solutuions can be stored for up to
1 year at RT (15-25℃)
Storage
3
DNA Purification Kits
E-mail: [email protected] Fax: +86-20-87791381 42
DN
A P
uri
fic
ati
on
Kit
s
N1012
100preps
300µl
30ml
30ml
40ml
60ml
2x30ml
10ml
100each
N1013
200preps
600µl
60ml
60ml
80ml
120ml
3x40ml
20ml
200each
Plasmid Extraction Series
Genomic DNA Purification Serious
Quick Genomic DNA Extraction Kit
Blood Genomic DNA Extraction Kit
Quick Blood Genomic DNA Extraction Kit
Quick Tissue/Culture Cells Genomic DNA Extraction Kit
Quick Bacteria Genomic DNA Extraction Kit
Quick Yeast Genomic DNA Extraction Kit
Marine Animal Tissue Genomic DNA Extraction Kit
Product Name Cat # Size
N1111
N1112
N1121
N1122
N1131
N1132
N1141
N1142
N1151
N1152
N1161
N1162
N1171
N1172
50 preps
100 preps
50 preps
100 preps
50 preps
100 preps
50 preps
100 preps
50 preps
100 preps
50 preps
100 preps
50 preps
100 preps
http://dongshengbio.com Tel: +86-20-8779135641
Product Name Cat # Size
High Purity Plasmid Miniprep kit
Eco-Friendly Plasmid Miniprep Kit
Plasmid DNA Maxiprep Kit (High Purity, Silica-mimbrane
Spin Column)
Plasmid DNA Maxiprep Kit (High Purity, Silica preciptation)
Plasmid DNA Maxiprep Kit (Endotoxin-free)
Yeast Plasmid DNA Miniprep Kit
N1011
N1012
N1021
N1022
N1031
N1041
N1051
N1061
N1062
50 preps
100 preps
50 preps
100 preps
10 preps
10 preps
10 preps
50 preps
100 preps
PCP Product Purification Series
Gel Extraction Kit
Eco-Feiendly Gel Extraction Kit
PCR and DNA Fragment Purification Kit
Product Name Cat # Size
N1071
N1072
N1081
N1082
N1091
N1092
50 preps
100 preps
50 preps
100 preps
50 preps
100 preps
3
DN
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uri
fic
ati
on
Kit
s
DNA Purification Kits
Related Products
Product Name Cat # Size
Proteinase K (20mg/ml)
Proteinase K
(Lyophilized powder)
RNase A (20mg/ml)
RNase A (100mg/ml)
RNase A
(Lyophilized powder)
Dnase I
N9011
N9016
N9017
N9041
N9042
N9046
N9047
N9066
1ml
100mg
1g
1ml
1ml
100mg
1g
1g
High Purity Plasmid Miniprep Kit
Resuspend CellsLyse andNeutralize
Bind DNA
Wash column
Elute pure DNA
Description
High Purity Plasmid Miniprep Kit is designed
for rapid and cost-effective small-scale pre-
paration of high quality plasmid DNA from
recombinant E. Coli cultures. The kit utilizes
an exclusive silicabased membrane techno-
logy in the form of a convenient spin column.
Each spin column can recover up to 40ug of
plasmid DNA. The kit can be used for any size
plasmid and cosmids purification efficiently.
Content
Size
RNase A
Resuspension Solution
Lysis Solution
Neutralization Solution
Wash Buffer PB
Wash Buffer W
Elution Buffer
(10mM Tris-HCl, pH 8.5)
Mini Spin Columns
N1011
50preps
150
15ml
15ml
20ml
30ml
30ml
5ml
50each
µl
For the initial use of the kit, dilute the Wash
Buffer W with ethanol 1:1.5 (v/v).
Figure .A 5 kb PCR fragment was cloned into pCMV vector. Lane M: 1kb ladderLane 1and 2 : Plasmid DNA from overnight bacterial cultures of recombinant clones was purified using the Plasmid Miniprep Kit (N1011). Lane 3 and 4 : analyzed by double digestion with EcoRI and HindIII in 30 min at 37℃.
M 1 2 3 4 M
Feature
Fast-procedure takes only 30 min
Efficient-can get 10-40 g high copy plas-
mid DNA in one prep
High purity-purified DNA is ready for down-
stream applications
µ
Application
Fast isolat ion of high purity plasmid DNA are
ready for di rect use in al l appl icat ions, inc lud ing:
Res triction digestion
DNA Seq uencing
In vitro transcript ion
Store RNase at -20℃.
Other solutuions can be stored for up to
1 year at RT (15-25℃)
Storage
3
DNA Purification Kits
E-mail: [email protected] Fax: +86-20-87791381 42
DN
A P
uri
fic
ati
on
Kit
s
N1012
100preps
300µl
30ml
30ml
40ml
60ml
2x30ml
10ml
100each
N1013
200preps
600µl
60ml
60ml
80ml
120ml
3x40ml
20ml
200each
Gel Extraction Kit
Excise gel slice with DNA fragment
Add Binding Solution to the gel slice. Incubate 10 min at 50-60℃
Transfer the mixture to the Quick Gel Extraction Columns
CentrifugeDiscard supernatant
Resuspend in wash bufferCentrifugeDiscard wash bufferRepeat 2X
Resuspend in TE bufferIncubate 5 min at 65
Centrifuge and store the micr-ocentrifuge tube containing theeluted DNA at 4 or 20
Content
Size
Binding Buffer BD
Wash Buffer PE
Elution Buffer
(10mM Tris-HCl,pH 8.5)
Spin Columns
N1071
50preps
20ml
15ml
2.5ml
50each
N1072
100preps
40ml
2x15ml
5ml
100each
For the initial use of the kit, dilute the Wash
Buffer PE with ethanol 1:4 (v/v)Feature
Fast-procedure takes only 15 min.
Efficient-gather up to 85% recoveries in
the range of 50bp-40kb.
Safe-not involve phenol extraction
High Purity-purified DNA is ready for
downstream applications.
Fast and efficient extraction of high purity
DNA fragments are ready for direct use in
all applications, including:
Storage
All solutions can be stored for up to 1year
RT (15-25℃).
Restriction digestion
PCR
DNA sequencing
In vitro transcription
Application
TMFigure . Recovery of DS 5000 individual 100-5000 bpDNA fragments from a 1% agarose gel using the DNA Gel M 1 2 3 4 5 6 7 8 9 M
TMLane M: DS 5000 ; Lane1:100bp; Lane 2: 250bp;Lane 3: 500bp; Lane 4: 750bp; Lane 5: 1000bp; Lane 6: 1500bp; Lane 7: 2000bp; Lane 8: 3000bp; Lane 9: 5000bp.
.Extraction Kit.
Description
Gel Ext raction Ki t is dees igned to extract and
purify DNA fragments of 50bp to 40kb from s
tandard or low- mel t agarose ge ls in ei ther Tris
acetate (TAE) or Tris borate (TBE) . Thi s mem-
brane-based system, whi ch can bind up to
40ug DNA, al lows recovery of isolated DNA
fragments in as little as 20 minutes.
3
http://dongshengbio.com Tel: +86-20-8779135643
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ati
on
Kit
s
N10732
200preps
80ml
3x20ml
10ml
200each
DNA Purification Kits
E-mail: [email protected] Fax: +86-20-87791381 44
3
DN
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uri
fic
ati
on
Kit
ss
Quick Blood Genomic DNA Extraction Kit
Content
Size
Solution DS
Solution QS
Proteinase K
Wash Buffer PS
Wash Buffer PE
Elution Buffer
(10mM Tris-HCl, pH 8.5)
Spin Columns
N1131
50preps
15ml
20ml
1ml
30ml
15ml
5ml
50each
N1132
100preps
30ml
40ml
2ml
60ml
30ml
10ml
100each
For the initial use of the kit, dilute the Wash
Buffer PE with ethanol 1:3 (v/v).
Feature
Fast--procedure takes only 30 min
High Efficient--5 g D
Simple--no need to remove erythrocyte from
the whole blood
Safe--no phenol extraction step
µ NA from 200µl whole
blood
Fast and efficient extraction of high
purity DNA fragments are ready for
direct use in all applications, including:
Storage
Store proteinase K at -20℃. Other solutions
can be stored for up to 1year at RT (15-25℃).
Restriction digestion
PCR
labeling
In vitro transcription
Application
Resuspend CellsLyse andNeutralize
Bind DNA
Wash column
Elute pure DNA
bloodSample preparation
Figure . Extract chicken blood DNA and human blood DNA by using the Quick Blood Genomic DNA Extraction Kit.
Lane M: Lambda DNA/Lane1: chicken blood DNALane 2: Lane 3: human blood DNALane 4:
Hind III
EcoR I restriction product for purified chicken blood DN
EcoR I restriction product for purified human blood DNA
M 1 2 3 4 M
Description
Quick Blood Genomic DNA Extraction Kit
provides a simple and rapid method for high
quality genomic DNA purification from whole
blood. The Genomic DNA system uses the
silica-gel-menbrane technology for simple
and fast isolation for genomic DNA without
phenol/choroform.
Gel Extraction Kit
Excise gel slice with DNA fragment
Add Binding Solution to the gel slice. Incubate 10 min at 50-60℃
Transfer the mixture to the Quick Gel Extraction Columns
CentrifugeDiscard supernatant
Resuspend in wash bufferCentrifugeDiscard wash bufferRepeat 2X
Resuspend in TE bufferIncubate 5 min at 65
Centrifuge and store the micr-ocentrifuge tube containing theeluted DNA at 4 or 20
Content
Size
Binding Buffer BD
Wash Buffer PE
Elution Buffer
(10mM Tris-HCl,pH 8.5)
Spin Columns
N1071
50preps
20ml
15ml
2.5ml
50each
N1072
100preps
40ml
2x15ml
5ml
100each
For the initial use of the kit, dilute the Wash
Buffer PE with ethanol 1:4 (v/v)Feature
Fast-procedure takes only 15 min.
Efficient-gather up to 85% recoveries in
the range of 50bp-40kb.
Safe-not involve phenol extraction
High Purity-purified DNA is ready for
downstream applications.
Fast and efficient extraction of high purity
DNA fragments are ready for direct use in
all applications, including:
Storage
All solutions can be stored for up to 1year
RT (15-25℃).
Restriction digestion
PCR
DNA sequencing
In vitro transcription
Application
TMFigure . Recovery of DS 5000 individual 100-5000 bpDNA fragments from a 1% agarose gel using the DNA Gel M 1 2 3 4 5 6 7 8 9 M
TMLane M: DS 5000 ; Lane1:100bp; Lane 2: 250bp;Lane 3: 500bp; Lane 4: 750bp; Lane 5: 1000bp; Lane 6: 1500bp; Lane 7: 2000bp; Lane 8: 3000bp; Lane 9: 5000bp.
.Extraction Kit.
Description
Gel Ext raction Ki t is dees igned to extract and
purify DNA fragments of 50bp to 40kb from s
tandard or low- mel t agarose ge ls in ei ther Tris
acetate (TAE) or Tris borate (TBE) . Thi s mem-
brane-based system, whi ch can bind up to
40ug DNA, al lows recovery of isolated DNA
fragments in as little as 20 minutes.
3
http://dongshengbio.com Tel: +86-20-8779135643
DN
A P
uri
fic
ati
on
Kit
s
N10732
200preps
80ml
3x20ml
10ml
200each
DNA Purification Kits
E-mail: [email protected] Fax: +86-20-87791381 44
3
DN
A P
uri
fic
ati
on
Kit
ss
Quick Blood Genomic DNA Extraction Kit
Content
Size
Solution DS
Solution QS
Proteinase K
Wash Buffer PS
Wash Buffer PE
Elution Buffer
(10mM Tris-HCl, pH 8.5)
Spin Columns
N1131
50preps
15ml
20ml
1ml
30ml
15ml
5ml
50each
N1132
100preps
30ml
40ml
2ml
60ml
30ml
10ml
100each
For the initial use of the kit, dilute the Wash
Buffer PE with ethanol 1:3 (v/v).
Feature
Fast--procedure takes only 30 min
High Efficient--5 g D
Simple--no need to remove erythrocyte from
the whole blood
Safe--no phenol extraction step
µ NA from 200µl whole
blood
Fast and efficient extraction of high
purity DNA fragments are ready for
direct use in all applications, including:
Storage
Store proteinase K at -20℃. Other solutions
can be stored for up to 1year at RT (15-25℃).
Restriction digestion
PCR
labeling
In vitro transcription
Application
Resuspend CellsLyse andNeutralize
Bind DNA
Wash column
Elute pure DNA
bloodSample preparation
Figure . Extract chicken blood DNA and human blood DNA by using the Quick Blood Genomic DNA Extraction Kit.
Lane M: Lambda DNA/Lane1: chicken blood DNALane 2: Lane 3: human blood DNALane 4:
Hind III
EcoR I restriction product for purified chicken blood DN
EcoR I restriction product for purified human blood DNA
M 1 2 3 4 M
Description
Quick Blood Genomic DNA Extraction Kit
provides a simple and rapid method for high
quality genomic DNA purification from whole
blood. The Genomic DNA system uses the
silica-gel-menbrane technology for simple
and fast isolation for genomic DNA without
phenol/choroform.
3
Website: www.dongshengbio.com Tel: +86-20-8779135645
DN
A P
uri
fic
ati
on
Kit
s
Proteinase K
Storage
N9011:1 year at room temperature, 2 years at -20℃.
N9016/N9017: 2 years at 4℃
Description
Proteinase K is an endolyt ic prot ease that cleaves
pept ide bonds at the carbox yl ic sides of al iphat ic,
arom at ic or hydrop hobic amino ac ids. The Proteinase
K is classi fied as a serine prot ease. The smal lest
pept ide to be hydrol yze d by this enzym e is a tet rapeptide.
Code
N9011
N9016
N9017
Product
Proteinase K (20mg/ml)
Proteinase K
(Lyophilized powder)
Proteinase K
(Lyophilized powder)
Size
1ml
100mg
1g
R
Storage
N9041/N9042:1 year at RT, 2 years at -20℃.
N9016/N9047: 2 years at 4℃
Description
RNase A, Dnase and prot ease-free is an endoribonuclease
that spec ifica lly degrad es sing le-st randed RNA at C and U
res idues. It cleaves the phosphodiester bond between the
5’ -ribose of a nucleotide and the phosphate grou p at tached
Code
N9041
N9042
N9046
N9047
Product
RNase A (20mg/ml)
RNase A (100mg/ml)
RNase A
(Lyophilized powder)
RNase A
(Lyophilized powder)
Size
1ml
1ml
100mg
1g
Nase A
to the 3\-ribose of an adjacent primidine nucleotide.
D
Storage
: 2 years at 4℃
Description
Dnase I, (RNase-fre e) is an endonuclease that nonspe-
cifically cleaves DNA to re lease di-, tri - and oligonucleotide
products with 5’-phosphory lated and 3’-hydroxylated ends.
DNase I acts on single- and double- stra nded DNA, chromatin
Code
N9066
Product
DNase I
(Lyophilized powder)
Size
1g
Nase I
Specificity activity:≥ 200Kunitz units/mg
and RNA:DNA hybrids.
Specificity activity:≥ 100Kunitz units/mg protein
Specificity activity: 40U/mg protein
DNA Purification Kits
E-mail: [email protected] Fax: +86-20-8779138126
3
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Pro
du
cts
4. RNA Purification Kits
3
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Kit
s
Proteinase K
Storage
N9011:1 year at room temperature, 2 years at -20℃.
N9016/N9017: 2 years at 4℃
Description
Proteinase K is an endolyt ic prot ease that cleaves
pept ide bonds at the carbox yl ic sides of al iphat ic,
arom at ic or hydrop hobic amino ac ids. The Proteinase
K is classi fied as a serine prot ease. The smal lest
pept ide to be hydrol yze d by this enzym e is a tet rapeptide.
Code
N9011
N9016
N9017
Product
Proteinase K (20mg/ml)
Proteinase K
(Lyophilized powder)
Proteinase K
(Lyophilized powder)
Size
1ml
100mg
1g
R
Storage
N9041/N9042:1 year at RT, 2 years at -20℃.
N9016/N9047: 2 years at 4℃
Description
RNase A, Dnase and prot ease-free is an endoribonuclease
that spec ifica lly degrad es sing le-st randed RNA at C and U
res idues. It cleaves the phosphodiester bond between the
5’ -ribose of a nucleotide and the phosphate grou p at tached
Code
N9041
N9042
N9046
N9047
Product
RNase A (20mg/ml)
RNase A (100mg/ml)
RNase A
(Lyophilized powder)
RNase A
(Lyophilized powder)
Size
1ml
1ml
100mg
1g
Nase A
to the 3\-ribose of an adjacent primidine nucleotide.
D
Storage
: 2 years at 4℃
Description
Dnase I, (RNase-fre e) is an endonuclease that nonspe-
cifically cleaves DNA to re lease di-, tri - and oligonucleotide
products with 5’-phosphory lated and 3’-hydroxylated ends.
DNase I acts on single- and double- stra nded DNA, chromatin
Code
N9066
Product
DNase I
(Lyophilized powder)
Size
1g
Nase I
Specificity activity:≥ 200Kunitz units/mg
and RNA:DNA hybrids.
Specificity activity:≥ 100Kunitz units/mg protein
Specificity activity: 40U/mg protein
DNA Purification Kits
E-mail: [email protected] Fax: +86-20-8779138126
3
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4. RNA Purification Kits
RNA Purification Kits
Ribonucleic acid (RNA) is a biologically important type of molecule that
consists of a long chain of nucleotide units. RNA is transcribed from DNA
by enzyme called RNA Polymerase and is generally further processed
Obtaining high quality, intact RNA is the first and often the most critical
step in performing many fundamental molecular biology experiments,
including Northern analysis, nuclease protection assays, RT-PCR, RNA
mapping, in vitro translation and cDNA library construction. To be success-
ful, however, the RNA isolation procedure should include some important
Dongsheng Biotech offers an expansive portfolio of RNA Purification
products that now includes purification and stabilization products. Our
unique and trusted TRAzol, General RNA Extraction technologies provide
high yields and quantity demanded by our RNA Research team. We offer
products for RNA extraction and stabilization, as well as for purifying total
by other enzymes. RNA is central to Protein synthesis.
RNA Summarization
steps both before and after and actual RNA purification.
RNA from animals, plants, bacterias and cells.
RN
A P
uri
fic
ati
on
Kit
s
4
.http://dongshengbio.com Tel: +86-20-8779135647
RNA Purification Kits
E-mail: [email protected] Fax: +86-20-87791381
4
RN
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Kit
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48
TRAzol
TRAzol
R1021
20ml1
R1022
100ml
Storage
Store at 2-8℃, propect from light.
TRAzol is stable for up to 12 months when
properly stored.
The isolated RNA is suitable for any
downstream application, including:
RT-PCR
Amplification for array analysis
Hybridization assays
In vitro translation
Application
Reagents required, but not supplied.
Chloroform
Isopropyl alcohol
75% ethanol (RNA-free)
DEPC-treated water
.
.
RNA yield with different starting materials.
Starting Material
Liver tissue
Spleen tissue
Kidney tissue
Skeletal muscle or
brain tissue
Placenta
Cultured epithelial cells
Fibroblasts
Feature
Classical-used for classical RNA isolation
step
High Efficient-stable RNA yield
High Purity-purified RNA is ready for any
downstream application
Description
TRAzol Reagent is a ready-to-use reagent for
the isolation of total RNA from cells and tiss-
ues. The reagent, a mono-phasic solution of
phenol and guanidine isothiocyanate, is an
improvement the single-step RNA isolation
method. During sample homogenization or
lysis, TRAzol Reagent maintains the integrity
of the RNA, while disrupting cells and dissol-
ving cell components. TRAzol Reagent facil-
itates isolation of a variety of RNA species
of large or small molecular size.
Starting
material can be either fresh or frozen tissue, or cell samples of almost any size.
RNA Purification Kits
Ribonucleic acid (RNA) is a biologically important type of molecule that
consists of a long chain of nucleotide units. RNA is transcribed from DNA
by enzyme called RNA Polymerase and is generally further processed
Obtaining high quality, intact RNA is the first and often the most critical
step in performing many fundamental molecular biology experiments,
including Northern analysis, nuclease protection assays, RT-PCR, RNA
mapping, in vitro translation and cDNA library construction. To be success-
ful, however, the RNA isolation procedure should include some important
Dongsheng Biotech offers an expansive portfolio of RNA Purification
products that now includes purification and stabilization products. Our
unique and trusted TRAzol, General RNA Extraction technologies provide
high yields and quantity demanded by our RNA Research team. We offer
products for RNA extraction and stabilization, as well as for purifying total
by other enzymes. RNA is central to Protein synthesis.
RNA Summarization
steps both before and after and actual RNA purification.
RNA from animals, plants, bacterias and cells.
RN
A P
uri
fic
ati
on
Kit
s
4
.http://dongshengbio.com Tel: +86-20-8779135647
RNA Purification Kits
E-mail: [email protected] Fax: +86-20-87791381
4
RN
A P
uri
fic
ati
on
Kit
s
48
TRAzol
TRAzol
R1021
20ml1
R1022
100ml
Storage
Store at 2-8℃, propect from light.
TRAzol is stable for up to 12 months when
properly stored.
The isolated RNA is suitable for any
downstream application, including:
RT-PCR
Amplification for array analysis
Hybridization assays
In vitro translation
Application
Reagents required, but not supplied.
Chloroform
Isopropyl alcohol
75% ethanol (RNA-free)
DEPC-treated water
.
.
RNA yield with different starting materials.
Starting Material
Liver tissue
Spleen tissue
Kidney tissue
Skeletal muscle or
brain tissue
Placenta
Cultured epithelial cells
Fibroblasts
Feature
Classical-used for classical RNA isolation
step
High Efficient-stable RNA yield
High Purity-purified RNA is ready for any
downstream application
Description
TRAzol Reagent is a ready-to-use reagent for
the isolation of total RNA from cells and tiss-
ues. The reagent, a mono-phasic solution of
phenol and guanidine isothiocyanate, is an
improvement the single-step RNA isolation
method. During sample homogenization or
lysis, TRAzol Reagent maintains the integrity
of the RNA, while disrupting cells and dissol-
ving cell components. TRAzol Reagent facil-
itates isolation of a variety of RNA species
of large or small molecular size.
Starting
material can be either fresh or frozen tissue, or cell samples of almost any size.
http://dongshengbio.com Tel: +86-20-8779135649
4
RN
A P
uri
fic
ati
on
Kit
s
General RNA Extraction Kit
Feature
High Efficient-Stable RNA yield.
Reliable performance of high-quality
purified total RNA in downstream appli-
cations.
Storage
Store at 2-8℃, propect from light.
Kit contents are stable for up to
12 months when properly stored.
Content
Size
Solution RL
Wash Buffer RPI
Wash Buffer RW
DEPC-treated water
Rnase-free spin culumn
RNase-free
microcentrifuge tube
R1051
50preps
60ml
18ml
12ml
10ml
50each
50each
The isolated RNA is suitable for any down-
stream application, including:
RT-PCR
Amplification for microarray analysis
Hybridization assays
In vitro transation
Application
Reagents required, but not supplied.
Chloroform
Ethanol (96-100%)
For the initial use of the kit, dilute the Wash
Buffer RPI with ethanol 3:2 (v/v), and dilute
the Wash Buffer RW with ethanol 1:4(v/v).
1 2 3 4 Figure . Extract different species of total RNAby using the Quick Genomic DNA Extraction Kit.
Lane1: Mouse tissue RNA
Lane 2: Zebrafish tissue RNA
Lane3: Leguminosae leaf tissue RNA
Lane4: Nicotianatabacum leaf tissue RNA
Description
Gen eral RNA Extraction Ki t can isolate
total RNA from a wide range of sampl e
types and amou nts. In general, sampl es
are lysed and then homog enized in the
presence of guanidinium isothiocyanate,
a chaotropic salt capable of protecting
the RNA from endogenous RNa se. Af ter
homog enization, ethanol is added to the
sampl e. The sampl e is then processed
through a spin column containing a clean
silica-based membr ane to which the RNA
binds. Any impu rities are effectively rem-
oved by subsequent washing. The purified
total RNA is then eluted in RNa se-free
water.
5. Silica Spin Column
Code
Mini Spin Column I C1011 1000pcs 45 µg
5. Silica Spin Column
Features
Silica membrane based spin column
Compatible with hot brand extraction buffers
Economical for large sample process
High binding capability
High purity of DNA
Wide range of DNA fragment size
Convenient for use
Introduction
Dongsheng Biotech Silica Spin Columns are packed with glass fiber membranes to
rapidly purify cucleic acid using a glass fiber-based extraction method. These columns
can be used to purify single- or double- stranded DNA/RNA from contaminarlts such as
salts, solvents, enzymes or proteins. Nucleic acids are bound to the glass fiber under
high chaotropic salt concentrations, salts and umpurities are removed during the wash
steps. Pure DNA/RNA is eluted from the membrane using water or TE buffer.
Size Binding Ability Application
Purification of PCR products
Extraction of Agarose gel
Extraction of Genomic DNA
Extraction of Plasmid DNA
Extraction of Plasmid DNA
Extraction of Genomic DNA
Extraction of Plasmid DNAMaxi Spin Column C3011 100pcs
Mini Spin Column II C1021 1000pcs 50 µg
Mini Spin Column III C1031 1000pcs 55 µg
1000 µg
Product
5
Sil
ica
Sp
in C
olu
mn
50E-mail: [email protected] Fax: +86-20-87791381
Silica Spin Column
http://dongshengbio.com Tel: +86-20-8779135649
4
RN
A P
uri
fic
ati
on
Kit
s
General RNA Extraction Kit
Feature
High Efficient-Stable RNA yield.
Reliable performance of high-quality
purified total RNA in downstream appli-
cations.
Storage
Store at 2-8℃, propect from light.
Kit contents are stable for up to
12 months when properly stored.
Content
Size
Solution RL
Wash Buffer RPI
Wash Buffer RW
DEPC-treated water
Rnase-free spin culumn
RNase-free
microcentrifuge tube
R1051
50preps
60ml
18ml
12ml
10ml
50each
50each
The isolated RNA is suitable for any down-
stream application, including:
RT-PCR
Amplification for microarray analysis
Hybridization assays
In vitro transation
Application
Reagents required, but not supplied.
Chloroform
Ethanol (96-100%)
For the initial use of the kit, dilute the Wash
Buffer RPI with ethanol 3:2 (v/v), and dilute
the Wash Buffer RW with ethanol 1:4(v/v).
1 2 3 4 Figure . Extract different species of total RNAby using the Quick Genomic DNA Extraction Kit.
Lane1: Mouse tissue RNA
Lane 2: Zebrafish tissue RNA
Lane3: Leguminosae leaf tissue RNA
Lane4: Nicotianatabacum leaf tissue RNA
Description
Gen eral RNA Extraction Ki t can isolate
total RNA from a wide range of sampl e
types and amou nts. In general, sampl es
are lysed and then homog enized in the
presence of guanidinium isothiocyanate,
a chaotropic salt capable of protecting
the RNA from endogenous RNa se. Af ter
homog enization, ethanol is added to the
sampl e. The sampl e is then processed
through a spin column containing a clean
silica-based membr ane to which the RNA
binds. Any impu rities are effectively rem-
oved by subsequent washing. The purified
total RNA is then eluted in RNa se-free
water.
5. Silica Spin Column
Code
Mini Spin Column I C1011 1000pcs 45 µg
5. Silica Spin Column
Features
Silica membrane based spin column
Compatible with hot brand extraction buffers
Economical for large sample process
High binding capability
High purity of DNA
Wide range of DNA fragment size
Convenient for use
Introduction
Dongsheng Biotech Silica Spin Columns are packed with glass fiber membranes to
rapidly purify cucleic acid using a glass fiber-based extraction method. These columns
can be used to purify single- or double- stranded DNA/RNA from contaminarlts such as
salts, solvents, enzymes or proteins. Nucleic acids are bound to the glass fiber under
high chaotropic salt concentrations, salts and umpurities are removed during the wash
steps. Pure DNA/RNA is eluted from the membrane using water or TE buffer.
Size Binding Ability Application
Purification of PCR products
Extraction of Agarose gel
Extraction of Genomic DNA
Extraction of Plasmid DNA
Extraction of Plasmid DNA
Extraction of Genomic DNA
Extraction of Plasmid DNAMaxi Spin Column C3011 100pcs
Mini Spin Column II C1021 1000pcs 50 µg
Mini Spin Column III C1031 1000pcs 55 µg
1000 µg
Product
5
Sil
ica
Sp
in C
olu
mn
50E-mail: [email protected] Fax: +86-20-87791381
Silica Spin Column