HONEY

Congratulations!

Honey is a Maltese, AmericanStaffordshire Terrier, Shih TzuMixIn the following pages, you will learn about:

• KEY BREEDS DETECTED

• KEY BREED HISTORY, APPEARANCE, & BEHAVIOR

• MDR1 SCREENING

• MIXED-BREED ANCESTRY

• HOW KEY PHYSICAL TRAITS ARE INHERITED

• ADDITIONAL INFORMATION

KEY BREEDS DETECTED

YOU HAVE A MALTESE, AMERICAN STAFFORDSHIRE TERRIER, SHIH TZU MIXThe Wisdom Panel computer algorithm performed over seven million calculations using 11 different models (from a singlebreed to complex combinations of breeds) to predict the most likely combination of pure and mixed breed dogs in thelast 3 ancestral generations that best fit the DNA marker pattern observed in Honey. The ancestry chart depicting thebest statistical result of this analysis is shown in the picture below.

Maltese MalteseMixedBreed

MixedBreed

American Staffs.Terrier

MixedBreed

ShihTzu*

MixedBreed

MalteseMixedBreed American Staffs.

Terrier MixShih Tzu

Mix

MalteseMix

Honey

American Staffs.Terrier / Shih Tzu Mix

Interested in the Mixed Breed ancestry? Look for the Mixed Breed Signatures page later in the report!

*Breed detected, however at a lower confidence. Such results are not included in accuracy calculations.

KEY BREED HISTORY, APPEARANCE & BEHAVIOR

MALTESEAll dogs should be considered individual animals. Because each is a product

of their unique environment and handling, they may exhibit different traits and

behaviors than those listed here.

HEIGHT:

8 - 10 in

WEIGHT (SHOW):

6.5 - 9 lb

WEIGHT (PET):

5 - 13 lb

Mal

tese

EARS MUZZLE TAIL

The history of this breed traces back at least twenty-eight centuries and the Maltese is one of the few known breeds tohave retained its name of origin. The Roman Emperor Claudius, and Rome’s Governor of Malta, Publius each owned aMaltese. Publius was known to indulge his devotion to his pet with poetry and art immortalizing the dog. It was oncebelieved that the Maltese possessed curative powers. The dog was placed on a pillow and given the finest foods. TheMaltese was imported into Britain during the nineteenth century and became the preferred pet of the aristocracy. In 1888the breed was registered with the American Kennel Club and by the 1990’s, the Maltese was ranked in the top 15 of allbreeds, remaining a very popular breed in the United States today.

The Maltese has been bred to have little variation in appearance. It has silky long white hair which almost scrapes theground. Sometimes the ears have some slight lemon or tan coloring, though this is discouraged.

Do You Recognize Any of These Maltese Traits In Honey?

Intelligent, lively, and playful dogs.Maltese can be quick learners doing well with reward-basedtraining using treats or favorite toys.

Maltese can be energetic, although because of their smallsize they do well in apartments.

Can be quite gentle. Can also bark to defend itself fromstrangers or other animals.

KEY BREED HISTORY, APPEARANCE & BEHAVIOR

AMERICANSTAFFORDSHIRE TERRIER

All dogs should be considered individual animals. Because each is a product

of their unique environment and handling, they may exhibit different traits and

behaviors than those listed here.

HEIGHT:

17 - 19 in

WEIGHT (SHOW):

40 - 60 lb

WEIGHT (PET):

38 - 68 lb

Am

eric

an S

taffo

rdsh

ireTe

rrie

r

EARS MUZZLE TAIL

The American Staffordshire Terrier can trace its roots all the way back to the nineteenth century in England. The EnglishStaffordshire Terrier was created when various terriers were crossed with the Bulldog. The crossbreeding resulted in theactive and powerful breed that came to the attention of the United States public in 1870. American breeders then focusedtheir attention and efforts to increasing the size and weight of the American Staffordshire Terrier. These breeding effortsresulted in a Staffordshire Terrier that was recognized as a separate breed by the American Kennel Club in 1936. In1972, the current name of “American Staffordshire Terrier” was adopted. The American Staffordshire Terrier was onceused primarily for fighting. That practice, however, was banned in the early 1900’s and two separate variations of thebreed were gradually developed.

The American Staffordshire Terrier can be any color, with solid, particolor, or patched black, brown, tan, brindle (blackand brown striped), liver, red or fawn. Black or blue mask, brindle or tan points are commonly seen traits in this breed.Ears may also appear different due to cropping.

Do You Recognize Any of These American Staffordshire Terrier Traits In Honey?

Intelligent, hard-working, and stoic dogs.Very loyal to family and usually good with children. May notget along well with other dogs.

American Staffordshire Terriers seem to enjoy dog sportssuch as agility, flyball, rally and competitive obedience.

There have been reported incidents of American StaffordshireTerriers being aggressive with other pets or people.

KEY BREED HISTORY, APPEARANCE & BEHAVIOR

SHIH TZUAll dogs should be considered individual animals. Because each is a product

of their unique environment and handling, they may exhibit different traits and

behaviors than those listed here.

HEIGHT:

10 - 11 in

WEIGHT (SHOW):

10 - 18 lb

WEIGHT (PET):

10 - 21 lb

Shih

Tzu

EARS MUZZLE TAIL

The Shih Tzu was a breed that was created in Tibet during the seventeenth century. The development of the Shih Tzuwas achieved by the crossbreeding of the Lhasa Apso and Pekingese breeds. This crossbreeding occurred when a LhasaApso was given as a gift by the Dalai Lama to the Emperor of China. The Emperor had a Pekingese in his palace and thisPekingese mated with the Lhasa, resulting in the world’s first litter of Shih Tzu puppies. Eventually, the breed becamevery popular in the Imperial Court, but in 1908 after the death of Empress T’zu Hsi, most Shih Tzu breeding came toa stop. This was further complicated by the Communist Revolution in China, which nearly caused the breed to becomeextinct. The Shih Tzu was saved, however, by a pool of 14 dogs that were introduced into England. The Shih-Tzu did notfind its way to America until after World War II, when they were brought back with American soldiers. The breed receivedofficial American Kennel Club recognition in 1969.

The Shih Tzu is a breed with a wide variety of colors, with all permissible under the breed standard. The colors can beblacks, browns, silvers, creams, and brindle (black and brown pattern) in solid, particolor or tricolor with lots of white.Black markings or a black mask are also seen in this breed.

Do You Recognize Any of These Shih Tzu Traits In Honey?Alert, active, playful and are usually friendly dogs. Canparticipate in dog sports such as agility and rally orcompetitive obedience.

Stubborn tendencies may be lessened by using reward-basedtraining involving small treats and favorite toys.

Shih Tzus are moderately active when indoors and since theyare small do well in apartments.

May be intimidated by other dogs, causing defensive barkingleading to confrontations.

MIXED-BREED ANCESTRY

What Does the Mixed-breed Ancestry Mean for HONEY?

A portion of Honey’s ancestry was predicted to be mixed beyond the three generations we test for. It isdifficult to identify strong individual breed signals in this mixed portion, so we have listed the geneticbreed groups with the strongest statistical likelihood below. The breed groups are listed in order of strengthwith the most likely at the top of the list.

The most common ancestral trait of this group is being usedfor hunting. Some use acute powers of scent to follow a trailwhile others demonstrate the gift of stamina as they rundown a quarry. Beyond these two common traits, however,generalizations about hounds are hard to come by as the groupis comprised of a very diverse lot of breeds.

Example Breeds: Basset Hound, Beagle, Treeing WalkerCoonhound, Bloodhound

This group consists of dogs typically bred for the specificpurpose of human companionship, and many are popular petsbecause of their gentle nature. They became more common asthe concept and luxury of dogs as pets prevailed.

Example Breeds: Bichon Frise, Pug, Shih Tzu, Keeshond,Pomeranian

The Terrier Group ancestors were bred to hunt and kill vermin.They are often characterized as feisty and energetic dogswhose sizes range from fairly small to much larger.

Example Breeds: Russell Terrier, Soft-coated Wheaten Terrier,Standard Schnauzer, Chihuahua, Miniature Pinscher

The Asian Group is comprised mainly of breeds from the Asianand Arctic regions of the world. Often bred for guarding orworking they have been invaluable assets to man throughoutthe ages.

Example Breeds: Alaskan Malamute, Chinese Shar-Pei, ChowChow, Siberian Husky

Detection Threshold

MDR1 SCREENING

MDR1 Screening Results

Condition Gene Mode of Inheritance Test Results

Multi-Drug Sensitivity MDR1 Dominant Normal/Normal

Please be sure to share these results with your veterinarian at yourfirst opportunity. They may be critical to the health care of your dog.

Test Results AnalysisMDR1 Normal/Normal - These dogs have two copies of the normal MDR1 gene and do not have the MDR1mutation. They will not pass on the mutation to their offspring. These dogs are not at increased risk forexperiencing side effects from drugs that are pumped by P-glycoprotein.

About MDR1MDR1, or Multi-Drug Resistance-1 is a genetic mutation found in many of the herding breeds, some sight-houndbreeds and many mixed breed dogs. The MDR1 gene is responsible for production of P-glycoprotein, which is atransport pump that plays an important role in limiting drug absorption and distribution (particularly to the brain),and in enhancing the excretion of many drugs used in dogs.

Some dogs, particularly herding breeds or mixed-breed dogs with herding breed ancestry have a mutation in theMDR1 gene that makes them defective in their ability to limit drug absorption and distribution. These dogs are alsoslower to eliminate drugs from the body that are normally transported by P-glycoprotein. All dogs have two copiesof this gene, and dogs with mutations in both copies will be much more sensitive to many commonly used drugs.Even dogs with only one copy of the mutation, however, are more sensitive to drugs than dogs with two normalMDR1 genes. Therefore it is important to test your dog and share your results with your veterinarian so they canprovide your dog with the best possible care.

For more information about MDR1 and a full list of drugs affected by MDR1 please visit:http://www.wisdompanel.com/mdr1_disease_screening/ or http://vcpl.vetmed.wsu.edu

Origins of the TestThe discovery of the mutation of the multi-drug resistant gene (MDR1) and its effects on multi-drug sensitivityin dogs, was made by Washington State University. It is a patent-protected diagnostic test offered by WashingtonState University that has been licensed to Mars Veterinary for use in the Wisdom Panel tests.

HOW KEY PHYSICAL TRAITS ARE INHERITED

The Influence of Breeds on AppearanceThis report reveals the breeds detected in your dog’s DNA. It also includes checklists of physical traits associatedwith these breeds. Breeds can combine their influences in countless, fascinating ways. It’s the unique combinationthat makes your dog one-of-a-kind.

Your dog may behave like, or strongly resemble, just one of the breeds in its makeup. However much more commonlyyour dog’s looks and personality will blend aspects of these breeds. In the example below you can see how many ofFrankie’s physical traits are due to a blending of breed influences.

FEATHERINGThe longer hair on thelegs, tail and around theears, is due to dominantmodifier genes availablefrom the ShetlandSheepdog.

BLACK PIGMENTThis coloring in thenose, eye rims, lips andpads on the feet is dueto one copy of the blackgene variant, availablefrom all three ancestralbreeds.

WHITE SPOTTINGThis is due to a lack of pigment andis often found in the extremities (feet,chest, face, etc.), but can also extendover more of the body. It can be due tomany genes including those found inthe three ancestral breeds here.

BRINDLE COAT COLORThe black and tan striping in Frankie’scoat is a dominant trait coming fromone copy of the brindle gene variant.This is available from the ItalianGreyhound, Parson Russell Terrier andShetland Sheepdog.

SHORT HAIRThis is due to one copyof the gene variant fromthe Italian Greyhound orthe Parson Russell Terrierthat is dominant over thelong coat gene from theShetland Sheepdog.

Wisdom Panel found these breeds in our example dog Frankie:

For additional information, watch an illustrative video at http://youtu.be/UAcPOch4-C8 - it provideseducational content around canine DNA, the basic blueprint for life, and a closer look at inheritedgenes.

ADDITIONAL INFORMATION

Predicted Adult Weight ProfileBased upon the breeds detected in Honey’s ancestry and the genetic markers analyzed by Wisdom Panel, as well asthe information provided that Honey is female and spayed, we have calculated the likely weight that Honey shouldreach when an adult. The Wisdom Panel target weight profile is presented as a range based on the unique mix andproportion of breeds detected.

• The adult weight is predicted to be between 16 - 29 lbs.

Many factors can influence an adult dog’s weight, including sex and neuter status. Both of these factors have beenused to calculate the predicted weight profile. Another major influence on canine weight is body condition score,which is a factor of your dog’s lifetime nutrition and exercise regimen. For more information visit WisdomPanel.comand go to the "How it Works" section.

Talk to Your VeterinarianCongratulations on taking the steps to learn about Honey’s ancestry and to understandwhat breeds are in your mixed breed dog using Wisdom Panel! Knowing your dog’s breedsis more than a novelty, it is a medical necessity. Each breed in your dog's genetic pawprint may have unique health concerns you should know about so that you can providea lifetime of excellent care.

We recommend discussing your dog’s ancestry report and genetic disease screen results with your veterinarian, ashe or she can help you use your Wisdom Panel report to help better care for Honey. The next time you visit yourveterinarian, consider asking these questions in addition to any you come up with on your own:

• Are there any specific issues that may be relevant to my dog’s well-being?

• Are there any special techniques that may help in training a dog with this particular mix?

• Are there any particular dietary considerations to bear in mind for a dog like mine?

How Wisdom Panel WorksThe process started when you sent a sample to our laboratory, where the DNA was extracted from the cells andexamined for the 321 markers that are used in the test. The results for these markers were sent to a computer,that evaluated them using a program designed to consider all of the pedigree trees that are possible in the lastthree generations. The trees considered include a simple pedigree with a single breed (a likely pure-bred dog), twodifferent breeds at the parental level (a first-generation cross), all the way up to a complex tree with eight differentgreat-grandparent breeds allowed.

Our computer used information from over 200 breeds, types and varieties from our breed database to fill thesepotential pedigrees. For each of the millions of combinations of ancestry trees built and considered, the computer gaveeach a score representing how well that selected combination of breeds matched to your dog’s data. The pedigree withthe overall best score is the one which is shown on the ancestry chart. Only breeds that reached our set confidencethreshold for reporting are reported in the ancestry chart.

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...Or Another Kit for You!We'd like to say thank you for purchasing a Wisdom PanelDNA kit with $15 off another kit for you, or to pass alongto friends or family. Just visit WisdomPanel.com and usethis promo code* below at the time of checkout.

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Check Out the Dog Community!Want to share your results with other Wisdom Panelusers and dog lovers? We've got a Dog Community foryou! Just visit WisdomPanel.com and click on "DogCommunity" and you can search for dogs with the samemix of breeds as your own, or upload a photo and storyabout your own dog. It's easy, fun and a great way toshare your results.

Terms and ConditionsEach dog is unique and their physical and behavioral traits willbe the result of multiple factors, including genetics, training,handling, and environment. Using a proprietary analysis anddatabase, Wisdom Panel products can provide insight into thebreed ancestry of your dog, the predicted genetic adult weightrange, and breed-related risks of developing certain geneticdiseases. A dog’s weight range can vary significantly depending onage, diet, and exercise.

Wisdom Panel products are not intended to diagnose diseases orpredict behavior in any particular dog. Wisdom Panel products aredesigned and intended to be used solely to identify the geneticbreed makeup and predict the adult size of such a mixed-breeddog; to confirm the genetic profile observed in a tested dog matchesa particular breed profile; or in certain products to identify risks ofdeveloping certain genetic diseases or conditions. No other purposeis intended, authorized, or permitted.

Upon receipt of the sample, Mars Veterinary will analyze the dog’sDNA to determine the breed(s) present in its recent ancestry.In all cases this is done with a reasonable degree of certainty.Portions of DNA samples of some dogs may not include anypurebreds and those ancestors will simply be noted as being mixedbreeds. Wisdom Panel products are based upon the Mars Veterinarydatabase of validated breeds. If a dog’s ancestry contains breedsnot represented in our database, the results may identify relatedbreeds that are contained in the database.

In the unlikely event that it is not possible to determine breedhistory, predicted adult weight range, or breed related health risks,or that an error in the analysis occurs, liability by Mars Veterinaryor related companies and individuals is expressly disclaimed.Damages, if any, are limited to the payment actually received byMars Veterinary for the specified analysis at issue.

Mars Veterinary’s testing procedures are designed to providereliable and accurate results. Mars Veterinary is not responsible forany errors in obtaining the swab sample or for any injuries or lossthat may occur as a result.

Many countries and provinces have breed-specific ordinances andlaws that may require special handling or prohibit the ownershipof some dogs with a particular breed in their genetic background.Wisdom Panel is not intended to be used by regulatory oranimal control officials to determine whether a particular breedis legislated or banned in a particular country or province. Nor isWisdom Panel intended to be used in any judicial proceedings.Rather, it is intended to be used as a tool or resource in determininga dog's genetic history. Neither Mars Veterinary nor any relatedcompany is responsible for compliance or notification regardingthese matters.

Use of the tests acknowledges consent that Mars Veterinary mayuse any portion of the leftover sample, as well as the data collected,for research and development purposes only.

*This code is good for one-time use, on wisdompanel.com only. It cannot be combined with any other promotions or discounts and cannot be used on/in any other retailer site(s) or storefronts.

STATEMEN

T OF AUTH

ENTICATION

Ow

ner's name: David Edgin

Dog's nam

e: Honey

Date: January 30, 2016

This certifies the authenticity of Honey's canine genetic background

as determined follow

ing careful analysis of more than 3

00

genetic markers using W

isdom P

anel. The purebred dog breedsignature m

atches included in this analysis are those that were

detected in the last three generations of Honey's ancestry using

the proprietary breed detection algorithm at M

ars Veterinary.

Dr. Cynthia Cole DVM, PhD, DACVCP

Research &

Developm

ent Director

Maltese

Maltese

Mixed

Breed

Mixed

Breed

Am

erican Staffs.

TerrierM

ixedB

reedS

hihTzu*

Mixed

Breed

Maltese

Mixed

Breed

Am

erican Staffs.

Terrier Mix

Shih TzuM

ix

MalteseM

ix

Honey

Am

erican Staffs.

Terrier / Shih Tzu M

ix

Honey is a M

altese, American

Staffordshire Terrier, Shih Tzu Mix