confocal laser microscopy at the mhh. laser microscopy facility zentrale forschungseinrichtung...
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Confocal Laser Microscopy at the MHH
Confocal Laser Microscopy at the MHH
Laser Microscopy Facility
Zentrale Forschungseinrichtung Lasermikroskopie
Location:
Building I4, Level 01
Contact:
Rudolf Bauerfeind
Wolfgang Posselt
Tel.: -3914
Confocal Laser Microscopy at the MHH
Please,
visit the web page!
http://www.mh-hannover.de/lasermicroscopy.html
Confocal Laser Microscopy at the MHH
How does a confocal microscope work?
focal plane
confocal pinhole
confocalwide field
Confocal Laser Microscopy at the MHH
What is the advantage of using a confocal laser scanning microscope?
• optical sectioning
• 3D-imageing
• x-z sectioning
• better resolution
• easy multi colour fluorescence
• advantages of laser scanning
• bleaching (FRAP, …)
• ROI scan (photoactivatable
fluorescent proteins)
Katinka Döhner, Virology, MHH
Confocal Laser Microscopy at the MHH
Which fluorescent dyes can I use? Available laser lines [nm]
405
442
458
476
488
496
514
543
594
633
MP 710-920
The choice is only limited by the excitation wave length
Confocal Laser Microscopy at the MHH
Live cell imaging
• Incubation chambers with control of
temperature, humidity, and CO2
for cells grown on glass
• In situ microscopy using water immersion
long distance objectivesChristian Hähnel, Cell Biology
Confocal Laser Microscopy at the MHH
Microscopes and applications
Zeiss META
Leica inverted-2
Leica inverted-
MP
Leica Upright-
MP
Leica Upright-2
Olympus FV1000
Image Xpress
fixed samples
yes yes yes low
resolution yes yes yes
live cell imaging
37°C, CO2 37°C, CO2 37°C, CO2 low
resolution no 37°C, CO2 no
UV no yes no no no yes -
multi-photon
no no yes yes yes no -
good for fast live
cell blue DNA
stains FLIM, FCS
in situ: organs – animals
orange-red dyes (561nm)
slow live cell (hours – days); FRAP
high through
put screening
• The laser microscopy facility is a S2 laboratory
• A cell culture with 2 safety hoods and 2 incubators
can be used to prepare your experiments.
• Computer with full versions of the microscope software
and 3d-imaging software are available
Confocal Laser Microscopy at the MHH
Additional facilities
Confocal Laser Microscopy at the MHH
How can I use the facility?1. Send an e-mail: konfokal@mh-hannover.
2. We will discuss your project.
3. A training session will instruct you
in microscope operation and laser safety.
Bring your own samples!
4. You can use the microscope on your own.
Reservation are made through WWW.
The facility is accessible 24 hr.
5. User will be charged with a user fee:
3-5 Euros per hr for MHH scientists.