complex lipids. introduction: a 3 week premature baby boy born to a diabetic mother by cesarean...
TRANSCRIPT
Complex LipidsComplex Lipids
• Introduction: A 3 week premature baby boy born to a diabetic mother by cesarean section.
• Presenting complaints: Bluish discoloration of the skin and mucus membranes (cyanosis) with apnea.
• Examination: Unusual breathing movement -- drawing back of chest muscles with breathing. APGAR score less than 5
• Investigations: lecithin/sphingomyelin ratio of amniotic fluid at 34th week of gestation = 1.2
• Laboratory investigations: Blood gas analysis of baby indicates low oxygen and excess acid in the body fluids. Blood culture negative for infection.
• Diagnosis: Acute respiratory distress syndrome (ARDS)/ Infant respiratory distress syndrome (IRDS)
Vignette 3Vignette 3
LIPIDS
SIMPLELIPIDS
COMPLEXLIPIDS
ClassificationClassification - (Structure) - (Structure)
Fats and Fats and Oils Oils
WaxesWaxes PhospholipidsPhospholipids GlycolipidsGlycolipids
Glycero-Glycero-phospho-phospho-lipidslipids
Sphingo-Sphingo-phosphophospholipidslipids
Cerebro-Cerebro-sidessides
Globo-Globo-sidessides
Ganglio-Ganglio-sidessides
Sulfa-Sulfa-tidestides
Simple LipidsSimple Lipids
Triacylglyceride
FATTY ACID
FATTY ACID
FATTY ACID
Complex lipids: Complex lipids: PhospholipidsPhospholipids
GlycerophospholipidsGlycerophospholipids Ether Glycerolipids Sphingophospholipids
GlycerophospholipidsGlycerophospholipids
Phosphatidic acidPhosphatidic acid
FATTY ACID
FATTY ACID
PP
GlycerophospholipidsGlycerophospholipids
(16:0, 18:0)
(18:1, 18:2, 18:3)
PhosphatidylcholinePhosphatidylcholineLung surfactant = 90% lipids (Dipalmitoylphosphatidyl-choline, DPPC; Dipalmitoylecithin) + 10% protein
CardiolipinCardiolipin
DistributionDistribution::
Inner mitochondrial Inner mitochondrial membranemembrane
FunctionFunction::
Maintenance of Maintenance of respiratory respiratory complexescomplexes
Diphosphatidylglycerol
Ether GlycerolipidsEther Glycerolipids
PlasmalogensPlasmalogens
Distribution:
Phosphatidalethanolamine (in nerve tissue)
Phosphatidalcholine (in heart muscle)
Function:
More resistant to oxidative stress therefore provides protection to tissues with active aerobic metabolism
Platelet-activating factorPlatelet-activating factor
DistributionReleased by a variety of cell types, including platelets, neutrophils, basophils, and endothelial cells.FunctionsPAF activates inflammatory cells and mediates hypersensitivity, acute inflammatory, and anaphylactic reactions.
Phosphatidylinositol (PI)Phosphatidylinositol (PI)
Distributionpresent in all tissues and cell types. Especially abundant in brain tissue, (10% of the phospholipids).
Functions:Cell signaling,Reservoir of arachidonic acidProtein anchoring
Stearic acid (18:0)
Arachidonic acid (20:4)
Phosphatidylinositol 4, 5 –Phosphatidylinositol 4, 5 –bisphosphate (PIP2)bisphosphate (PIP2)
Protein anchoringProtein anchoring
SphingophospholipidsSphingophospholipids
SphingophospholipidsSphingophospholipids
SphingosineSphingosine
2-amino-4-octadecene-1,3-diolC-18 alcohol containing two –OH groups, one amino group and one double bond
SphingomyelinSphingomyelin
DistributionConstituent of the myelin sheath of nerve fibers.FunctionsBuilding block of myelin sheathPrimary source of ceramideSignal transduction
Phospholipids - Phospholipids - DegradationDegradation
Niemann-Pick diseaseNiemann-Pick disease
• autosomal recessive disease
• inability to degrade sphingomyelin.
• deficiency of sphingomyelinase - a type of phospholipase C.
Glycolipids/Glycolipids/glycosphingolipidsglycosphingolipids
FATTY ACID
O SINE
CARBOHYDRATE
Glycolipids/Glycolipids/glycosphingolipidsglycosphingolipids
Glycolipids/Glycolipids/glycosphingolipidsglycosphingolipids
Distributionessential components of all membranes in the body. greatest amounts in nerve tissue
Functionsregulation of cellular interactions, growth, and developmentBlood group antigens
CEREBROSIDES
GLOBOSIDES
GANGLIOSIDES
SULFATIDES
CerebrosidesCerebrosides
• ceramide monosaccharides -simplest neutral glycosphingolipids
• Galactocerebroside - the most common cerebroside found in membranes
• Glucocerebroside - serves primarily as an intermediate in the synthesis and degradation of the more complex glycosphingolipids.
• cerebrosides are found predominantly in the brain and peripheral nervous tissue, with high concentrations in the myelin sheath
GalactocerebrosideGalactocerebroside
GlobosidesGlobosides
• Ceramide oligosaccharides
• Addition of monosaccharides (including GalNAc) to a glucocerebroside
e.g.
Cer-Glc-Gal (lactosylceramide)
Cer-Glc-Gal-Gal-GalNac-GalNac (Forssman antigen)
• Negatively charged at physiological pH
• Glycolipids containing sialic acid (N-acetylneuraminic acid, NANA)
• found primarily in the ganglion cells of the central nervous system, particularly at the nerve endings
GangliosidesGangliosides
Nomenclature• is based on the number of sialic acid residues
– 'GM' a single (mono) sialic acid, – GD, GT and GQ two, three and four sialic acid
residues in the molecule, respectively• on the sequence of the carbohydrates.
– The number after the GM, e.g. GM1 refers to the structure of the oligosaccharide.
– These numbers were derived from the relative mobility of the glycolipids on thin layer chromatograms; the larger, GM1, gangliosides migrate the most slowly.
GangliosidesGangliosides
• cerebrosides that contain sulfated galactosyl residues
• negatively charged at physiologic pH
• found predominantly in nerve tissue and kidney
SulfatidesSulfatides
• Defects in sequential degradation of glycolipids lead to a number of lysosomal storage diseases, Sphingolipidosis (cerebrosidoses and gangliosidoses)
• A specific lysosomal hydrolytic enzyme is deficient in each disorder. Therefore, usually only a single sphingolipid (the substrate for the deficient enzyme) accumulates in the involved organs in each disease
SphingolipidosisSphingolipidosis
THE END!THE END!