common reference intervals addition or takeover
TRANSCRIPT
![Page 1: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/1.jpg)
![Page 2: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/2.jpg)
• Working Party• Meeting 2013 and 2014• Australasian Involvement• Publications in Pipeline• Paediatric WP• Pregnant Ranges Evaluated• Recommendations for action
![Page 3: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/3.jpg)
• Results to Date• Tests added to List• Action by Laboratories• Battachyria analysis• Data extraction analysis• Median of Values obtained• Calculated Ranges
![Page 4: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/4.jpg)
• Survey results• Bias Assessment• Battacharyia• Aussie Normals• Manufacturers RI• Calculated Results Survey• Back to basics check
![Page 5: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/5.jpg)
0.0%
5.0%
10.0%
15.0%
20.0%
25.0%
30.0%
35.0%
4.8 4.9 5.0 5.1 5.2 5.3 5.4 5.5 5.6 5.7
Upper reference interval for Potassium mmol/L
4.8
4.9
5.0
5.1
5.2
5.3
5.4
5.5
5.6
5.7
![Page 6: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/6.jpg)
3.8
3.9
4
4.1
4.2
4.3
4.4
4.5
4.6
4.7
4.8
4.9
5
5.1
5.2
5.3
5.4
3.8 3.9 4 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 5 5.1 5.2 5.3 5.4
ADVIA 2400 Architect AU2700 DxCIntegra Modular RxL VitrosLinear (ADVIA 2400) Linear (Architect) Linear (AU2700) Linear (DxC)Linear (Integra) Linear (Modular ) Linear (RxL) Linear (Vitros)
![Page 7: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/7.jpg)
Potassium Variation
• Instrument Variation
• Pre Analytical Variables
• ISE Technology
• Precision of Assay
![Page 8: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/8.jpg)
ISE Nernst Equation
• Use of common equation
• Differences due to electrode factors
• Variations minimal
• CV 1-2% obtained
![Page 9: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/9.jpg)
0.0%
10.0%
20.0%
30.0%
40.0%
50.0%
60.0%
70.0%
95 100 105 110 115 120 125
Upper reference interval for Creatinine umol/L Male
95
100
105
110
115
120
125
![Page 10: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/10.jpg)
4048566472808896
104112120128136144152
40 48 56 64 72 80 88 96 104 112 120 128 136 144 152
Alan
lytic
al p
altfo
rm a
vera
ge c
once
ntra
tion
Average
Creatinine
ADVIA 2400 Architect AU2700 DxCIntegra Modular RxL VitrosLinear (ADVIA 2400) Linear (AU2700) Linear (DxC) Linear (Integra)Linear (Modular ) Linear (RxL) Linear (Vitros)
![Page 11: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/11.jpg)
Creatinine Standardisation
• Equation for eGFR – CKD - EPI• Standardisation of all methods• Various intercepts in place – method
dependent or are they?• Review of manufacturers calculation
parameters
![Page 12: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/12.jpg)
0.0%
5.0%
10.0%
15.0%
20.0%
25.0%
30.0%
35.0%
40.0%
40 45 50 55 60 65
Upper reference interval for GGT
40
45
50
55
60
65
![Page 13: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/13.jpg)
• Manufacturer Decision• Allowable Limits of Performance – Calibrator• Allowable limits for Performance – Extinction
Coefficients• Commonality of Calculation Factors• Method Variations
![Page 14: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/14.jpg)
Enzyme Calculation
• Manufacturer Dependent
• Calibration from Extinction Coefficient
• Calibration from Prepared Calibrator
• Dry Chemistry Estimation
![Page 15: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/15.jpg)
0
5
10
15
20
25
30
35
40
45
50
55
0 5 10 15 20 25 30 35 40 45 50 55
ADVIA 2400 Architect AU2700 DxCIntegra Modular RxL VitrosLinear (ADVIA 2400) Linear (Architect) Linear (AU2700) Linear (DxC)Linear (Integra) Linear (Modular ) Linear (RxL) Linear (Vitros)
![Page 16: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/16.jpg)
Enzyme Kinetics
• 1St Order Reaction• Zero Order Reaction• Initial Velocity Reaction• Km Values• Inhibitors – Competitive, Non Competitive and
Uncompetitive
![Page 17: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/17.jpg)
Colorimetric Reactions
• Optimisation of Chemistries• Wavelength Selection• Reaction reading times• Calibrator values• Calculation from data obtained
![Page 18: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/18.jpg)
![Page 19: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/19.jpg)
Reaction Traces
• End point reactions
• Rate Reactions
• Similarity between Manufacturers
• Choice of slope parameters
![Page 20: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/20.jpg)
• Abbott Diagnostics Architect• Roche Diagnostics Modular• Roche Diagnostics Integra• Beckman Coulter DxC• Beckman Coulter Olympus• Siemens Healthcare Dimension• Siemens Healthcare Advia• Ortho Clinical Diagnostics Vitros.
![Page 21: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/21.jpg)
0.0
5.0
10.0
15.0
20.0
25.0
30.0
0 5 10 15 20 25 30
Iron
Obt
aine
d
Iron Average
Iron
ADVIA2400Architect
AU2700
DxC
![Page 22: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/22.jpg)
5.0
10.0
15.0
20.0
25.0
30.0
5 10 15 20 25 30
Iron
Obt
aine
d
Iron Average
Iron
ADVIA 2400ArchitectAU2700DxCModularVitros
![Page 23: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/23.jpg)
• Decision ? Implement LDH L-P, ALT, Urate.
• Platform Suitability
• Manufacturer Involvement – LDH (L-P)
![Page 24: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/24.jpg)
• Involve Manufacturers
• Obtain Calculated Values for Comparison
• Comparisons with Population results
• Implement Standardised Calculation values
![Page 25: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/25.jpg)
• Gus Koerbin• Ken Sikaris• Graeme Jones• Maxine Reed• George Koumentakis• Jill Tate• Jan Gill• All who have participated in the 2013 Symposium• All involved with the Harmonisation Initiative
![Page 26: COMMON REFERENCE INTERVALS ADDITION OR TAKEOVER](https://reader031.vdocuments.us/reader031/viewer/2022020701/61f63d6538a8550a744863fd/html5/thumbnails/26.jpg)