common bleach dilutions

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Table 1. Common Bleach Dilutions Sodium hypochlorite % Bleach solution ratio Bleach dilution ppm (available chlorine) Comments **always use on cleaned surfaces 0.025% 1:200 1.5 tbs (0.6 oz.) bleach to 1 gallon water 250 ppm common household use 0.1% 1:50 1/8 cup (1 oz.) bleach to 1 gallon water 1,000 ppm commonly used 0.16% 1:32 1/2 cup (4 oz.) bleach to 1 gallon water 1,563 ppm commonly used 0.5% 1:10 1.5 cups (12 oz.) bleach to 1 gallon water 5,000 ppm very strong solution and should be used in a limited manner 3.33% 2:3 2 parts bleach to 3 parts water 33,333 ppm effective against foot-and- mouthdisease virus - use with caution Source: Glenda Dvorak Table 2. Characteristics of Selected Disinfectants Disinfectant Category Alcohols Aldehydes Biguanides Halogens: Hypochlorite Halogens: Iodine Compounds Oxidizing Agents Phenols Quaternary Ammonium Compounds (QAC) Sample Trade Names Ethyl alcohol Isopropyl alcohol Formaldehyde Glutaraldehyde Chlorhexidine Nolvasan Virosan Bleach Betadyne Providone Hydrogen peroxide Peracetic acid- Virkon S Oxy-Sept 333 One-Stroke Environ Pheno-Tek II Tek-Trol Roccal DiQuat D-256 Mechanism of Action Precipitates proteins • Denatures lipids • Denatures proteins • Alkylates nucleic acids • Alters membrane permeability • Denatures proteins • Denatures proteins Denature proteins and lipids • Denatures proteins • Alters cell wall permeability • Denatures proteins • Binds phos pholipids of cell membrane Advantages • Fast acting • Leaves no residue • Broad spectrum • Broad spectrum • Broad spectrum • Short contact time • Inexpensive • Stable in storage • Relatively safe • Broad spectrum • Good efficacy with organic material • Non- corrosive • Stable in storage • Stable in storage • Non- irritating to skin • Effective at high tempera tures and high pH (9- 10) Disadvantages • Rapid evaporation Flammable • Carcinogenic • Mucous membranes and tissue irritation • Only use in well ventilated areas • Only functions in limited pH range (5-7) • Toxic to fish (environmental concern) • Inactivated by sunlight • Requires frequent application • Corrodes metals • Mucous membrane and tissue irritation • Inactivated by QACs • Requires frequent application • Corrosive • Stains clothes and treated surfaces Damaging to some metals • Can cause skin and eye irritation Precautions Flamma ble Car cinogenic Never mix with acids; toxic chlorine gas will be released May be toxic to animals, especially cats and pigs

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7/28/2019 Common Bleach Dilutions

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Table 1. Common Bleach Dilutions

Sodium

hypochlorite %

Bleachsolution

ratio

Bleach dilutionppm

(available

chlorine)

Comments**always use on cleaned

surfaces

0.025% 1:200

1.5 tbs (0.6 oz.)

bleach to 1 gallonwater

250 ppm common household use

0.1% 1:501/8 cup (1 oz.)bleach to 1 gallon

water

1,000 ppm commonly used

0.16% 1:321/2 cup (4 oz.)bleach to 1 gallonwater

1,563 ppm commonly used

0.5% 1:101.5 cups (12 oz.)bleach to 1 gallonwater

5,000 ppmvery strong solution and shouldbe used in a limited manner

3.33% 2:32 parts bleach to 3parts water

33,333 ppm

effective against foot-and-

mouthdisease virus - use withcaution

Source: Glenda Dvorak

Table 2. Characteristics of Selected Disinfectants

Disinfectant

Category

Alcohols Aldehydes BiguanidesHalogens:

Hypochlorite

Halogens:

Iodine

Compounds

Oxidizing

Agents

Phenols

QuaternaAmmoniu

Compoun(QAC)

Sample TradeNames

EthylalcoholIsopropylalcohol

FormaldehydeGlutaraldehyde

ChlorhexidineNolvasan

Virosan

BleachBetadyneProvidone

Hydrogenperoxide

Peraceticacid-

Virkon SOxy-Sept

333

One-StrokeEnvironPheno-TekII Tek-Trol

RoccalDiQuat

D-256

Mechanism of Action

•Precipitates

proteins• Denatureslipids

• Denaturesproteins• Alkylatesnucleic acids

• Alters

membranepermeability

• Denaturesproteins

• Denaturesproteins

•Denatureproteinsand lipids

• Denaturesproteins

• Alters cellwallpermeability

• Denature

proteins• Binds phpholipids ocell

membrane

Advantages

• Fastacting• Leaves noresidue

• Broadspectrum

• Broadspectrum

• Broadspectrum• Shortcontact time

• Inexpensive

• Stable instorage• Relativelysafe

• Broadspectrum

• Goodefficacy with

organicmaterial• Non-corrosive

• Stable instorage

• Stable instorage• Non-

irritating toskin• Effective high tempe

tures andhigh pH (9

10)

Disadvantages

• Rapid

evaporation•Flammable

• Carcinogenic• Mucousmembranes

and tissueirritation• Only use inwell ventilated

areas

• Only

functions in

limited pHrange (5-7)• Toxic to fish

(environmentalconcern)

• Inactivatedby sunlight• Requires

frequent

application• Corrodesmetals

• Mucousmembraneand tissueirritation

• Inactivatedby QACs• Requiresfrequent

application• Corrosive• Stainsclothes and

treatedsurfaces

Damagingto somemetals

• Can causeskin and eyeirritation

Precautions Flammable Carcinogenic

Never mixwith acids;toxic chlorinegas will be

released

May be toxicto animals,especiallycats and

pigs

7/28/2019 Common Bleach Dilutions

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Table 2. Characteristics of Selected Disinfectants

Disinfectant

CategoryAlcohols Aldehydes Biguanides

Halogens:

Hypochlorite

Halogens:

IodineCompounds

Oxidizing

AgentsPhenols

QuaternaAmmoniu

Compoun(QAC)

VegetativeBacteria

Effective Effective Effective Effective Effective Effective Effective

Yes-GramPositive

Limited-Gram

Negative

Mycobacteria Effective Effective Variable Effective Limited Effective Variable Variable

EnvelopedViruses

Effective Effective Limited Effective Effective Effective Effective Variable

Non-envelopedViruses

Variable Effective Limited Effective Limited Effective Variable Not Effectiv

SporesNotEffective

Effective Not Effective Variable Limited Variable Not Effective Not Effectiv

Fungi Effective Effective Limited Effective Effective Variable Variable Variable

Efficacy with

Organic MatterReduced Reduced ?

Rapidly

reduced

Rapidly

reducedVariable Effective Inactivated

Efficacy withHard Water

? Reduced ? Effective ? ? Effective Inactivated

Efficacy with

Soap/Detergents? Reduced Inactivated Inactivated Effective ? Effective Inactivated