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AMNIVOR

Collagen

New Generation Collagen: Aquatic Source

The collagen is mainly produced from bovine, pig and avian based resources globally.

However, the wide range acceptability and usage of these sources is restricted because of

concern of immunogenicity, disease transmission and religious barrier.

Aquatic origin is found to be an alternate source of collagen worldwide as it delivers a wide

range of benefits like superior absorption, better bioavailability, and minimum chance of disease

transmission through protein impurities.

Among the aquatic sources, marine and freshwater fish sources are now emerging and

replacing bovine, pig and avian resources.

To meet the ongoing demands for safer, ethical and more environmentally friendly

alternate source of collagen, AMNIVOR Medicare is using freshwater fish as a novel source for

manufacturing Type I collagen (AMNICOL). This freshwater fish collagen has low molecular

weight and is natural, pure, colourless, highly stable with intact collagen fibrillar structure and safe

(insignificant immunogenicity). It is produced in an ethical and environmentally-sustainable

manner, widely accepted across the cultures and religions (Halal certification is not essential) and

suitable for most demanding applications.

Unique advantages of AMNICOL

DENATURATION TEMPERATURE (Td)

The denaturation temperature (Td) of collagen from marine source is low (edible jellyfish collagen

~ 26 °C; starfish collagen ~ 24.7 C). This is because of the lower hydroxyproline content of these

sources which is manifested by their habitat and body temperature. However, the denaturation

temperature (Td) of AMNICOL is high (~36 °C) and similar to mammalian originated collagen.

COMPATIBILITY

Marine collagen has superior absorption and bioavailability compared to bovine- or porcine-

sourced collagen. However, the sources are adapted to fresh water resulting in collagen that is

quite different from that found in terrestrial species.

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AMNIVOR

AMNICOL more closely resembles the amino acid profile of human skin compared to marine

sources and therefore, most of the delivered amino acids are viable, reaches to cells and can be

metabolized through various anabolic processes.

HIGHER PURITY

Marine collagen can be contaminated with heavy metals and other contaminants. However, the

source of AMNICOL is freshwater fish and hence it is free from heavy metals, fat or turbid matter,

carbs, sugar, and cholesterol

LESS ODOR AND TASTE

Marine collagen contains higher levels of sodium, which influences its taste. Freshwater fish

originated AMNICOL is naturally low in sodium and therefore, tasteless. The odor of AMNICOL is

less as compared to collagen from marine source

EFFICIENCY

AMNICOL contains amino acid profile similar to animal sourced collagen and therefore beneficial

for management of wound environment

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AMNIVOR

Amino acid Profile

Amino acid Abundance in mammal skin (residues/1000)

AMNIVOR Collagen (residues/1000)

Valine 22 22

Tyrosine 3 3

Tryptophan 0 0

Threonine 19 21

Serine 36 35

Proline 126 120

Phenylalanine 13 13

Methionine 6 14

Lysine 29 26

Leucine 24 23

Isoleucine 11 10

Hydroxyproline 95 80

Hydroxylysine 6 8

Histidine 5 4

Glycine 329 340

Glutamic acid 74 77

Cysteine 1 0

Aspartic acid 47 47

Arginine 49 50

Alanine 109 115

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AMNIVOR

APPLICATIONS

Health Care

Wound healing and therapeutic

tissue engineering

regenerative medicine

cell expansion and cell delivery vehicle

drug delivery system

simulated 3D microenvironment

reconstructive surgeries,

Rehabilitations of different damaged or diseased tissues.

scaffolds

Research

2D and 3D cell culture,

mimicking the in vivo situation in dynamic condition

laboratory reagents

transient 3D-matrix cell for cell expansion

dynamic cell culture

Functional Food & Beverage

substitute of nutrients

Amino acid supplements

ingredient of health drinks and dietary food

Cosmetic and Beauty Care

Improves skin elasticity

Enhances water absorption capacity

Aids in wrinkle reduction

moisturizer

Sheet, film, sponge, powder, particles, spray, gel etc.) for addressing different wound types (e.g.

chronic, diabetic, burn and surgical wound)

AMNIVOR Wound Dressings

Prepared in many forms (sheet, film, sponge, powder, particles, spray, gel etc.)

easy to use, highly conformable, patient friendly

Higher absorption capacity

addressing different wound types (e.g. chronic, diabetic, burn and surgical wound)

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AMNIVOR

Cover wounds of different sizes and nature, from condensed acute scrapings to spreading

chronic injuries, and treat lesions of any dimensions.

Presence of naturally occurring molecule as an antimicrobial and debridement agent.

Comparison of gel-electrophoresis pattern of Type I collagen - isolated from fresh water fish

(Amnivor Medicare) and calf skin (Sigma Aldrich, USA) (b) SEM microscopy of fish collagen

(a) (b)

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AMNIVOR

Products and forms

Collagen Powder Collagen Sheet

Collagen Sponge Collagen Staple Fiber

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AMNIVOR

Collagen Solution Collagen Flakes

Cell proliferation study while coated on a Petridis

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AMNIVOR

Electrophoretic patterns of Type I collagen obtained from(a) calf skin (Sigma Aldrich, USA)

and fish of mixed species and (b) Mrigel and mixed species.

FTIR spectrum of collagen obtained from Mix

Mix Mrigel

(a) (b)

Amide I

Amide II

Amide III

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AMNIVOR

SEM microscopy of the fabricated collagen scaffolds

AMNIVOR collagen is natural, contains no artificial colors, flavors,

additives, or preservatives. It is also free of fat, carbs, sugar, and

cholesterol and low in sodium. It is widely accepted across cultures

and religions.

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AMNIVOR

All Products Offered by Amnivor

Collagen Products

Collagen Forms

Bioink

3D Cell Culture Matrix

Haemostat

Suture

Dental Dressing

Wound Dressing

Collgen Forms

Analytical grade Reagent

Flake

Fiber

Powder

Solution

Sponge

Collagen Bioinks

Premix for 3D printing

Gel for 3D printing

3D Cell Culture Matrix of Collgen

Sponge

Beads

Sheet

Fibre

Gel

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AMNIVOR

Collagen Haemostat

Grannular

Sealant

Gel

Collagen coated suture

Antimicrobial radiopaque bioactive sture

Dental

Collagen plug

Collagen patch

Collagen tape

Collagen based wound Dressing

Particle

Powder

Film

Gel

Sheet

Sponge