cns studies with rodentpack2 telemetry system
DESCRIPTION
The rodentPACK2 transmits data via radiotelemetry, offering a well-tolerated, humane alternative to tethered EEG. Direct inputs from the device to cortical and deep brain regions provide a high signal-to-noise ratio, facilitating the detection of EEG events. The EEG analyzer module, within ecgAUTO software, allows scientist to easily: generate FFT power spectrums with user defined frequency bands detect epilepsy seizures differentiate between four sleep stages and calculate global sleep score.TRANSCRIPT
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cliquer pour ajouter un titreEpileptic seizure detection& sleep scoring
EEG telemetrysemi-invasive biopotential, temperature & activity in rodents
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software for CNS studiesrodentPACK2 EEG telemetry
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sources of EEG/EMG data
epilepsy seizure detection
sleep scoring
rodentPACK2 for EEG telemetry
using third party files and telemetry
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sources of EEG/EMG data
A
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emka SOFTWARE ACQUIRE & PROCESS DATA
• recorded by emka iox data acquisition software
• recorded from third party device with analog outputs
• converted from text file
• converted from third party acquisition software
• recorded directly from third party telemetry
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epileptic seizure detection
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EEG ANALYZER MODULEwithin ecgAUTO software
Automatic software seizure detection is peformed using combined analysis of various parameters
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EPILEPSY SEIZURE DETECTIONwithin ecgAUTO software
• detection is based on a user defined multicriteria condition involving
1 to 4 parameters and associated thresholds
• among these parameters, some may be derived from FFT computations
• analysis is performed in successive epochs of adjustable length
and overlap percentage
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EPILEPSY SEIZURE DETECTIONwithin ecgAUTO software
Example of detection settings:
• epochs of n seconds (typically 2 to 10)
• successive epochs overlap by x% (typically 0 to 50%)
• epoch is a candidate if its energy, or power within some frequency range (typically 3Hz to 10hz) exceeds a threshold
• seizure consists of a minimum number of consecutive candidate epochs (typically 2 to 3)
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EPILEPSY SEIZURE DETECTIONwithin ecgAUTO software
Epochs of 4s, with 50% overlap. Single threshold on total epoch energy.Main data table shows epoch status and detected seizures
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EPILEPSY SEIZURE DETECTION
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plot with individual spectrums shown along input EEG trace
EPILEPSY SEIZURE DETECTION
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EPILEPSY SEIZURE DETECTION
Detected seizures are visible:
• in main results table
• listed in a specific seizure table for review and edition
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SEIZURE SUMMARY TABLE
Seizure table shows:
• index
• time in file
• duration
• limits on trace
• individual epochs
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SEIZURE SUMMARY TABLE
Seizure table other key features:
• display seizures listed per time, or increasing/decreasing duration
• summary per time frame (number and total duration of all seizures in successive 15mn frames),
• status of criteria used to detect candidate epochs
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EPILEPSY SEIZURE EDITION
• manual edition of seizures limits is possible
• invalid seizures can be deleted
• undetected seizures can be created
• valid seizures can be exported to text file
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EPILEPSY SEIZURE EDITIONAutomatically detected limits (in green)
Manual cursor to modify limits (blue & red arrows)
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EPILEPSY SEIZURE EDITIONManually added seizure shown in yellow on trace and in summary table
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EPILEPSY SEIZURE INVALIDATIONInvalidated seizure in dotted red on trace, in pink in table
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EPILEPSY SEIZURE EDITION TRACKING
All manual edition actions:
• shown in summary table and on data traces• are recorded in data file• can be reversed
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EPILEPSY SEIZURE DATA EXPORT
Seizure text format table with date, time, seizure length
Full epoch per epoch table also available__________ __________ __________ epilepsy table report
cpu-date cpu-time site-time(hS01+)__EPL_epoch#(hS01+)__EPL_lengthS
january 7, 2009 15:49:13.000 00:01:21.991 17 65.59january 7, 2009 15:50:13.000 00:02:21.987 8 29.592january 7, 2009 15:53:09.000 00:05:17.991 41 157.599january 7, 2009 15:54:57.000 00:07:05.984 1 1.594january 7, 2009 15:57:37.000 00:09:45.974 5 17.593january 7, 2009 15:58:13.000 00:10:21.991 8 25.612january 7, 2009 15:59:01.000 00:11:09.988 10 37.591january 7, 2009 16:00:57.000 00:13:05.981 22 85.588january 7, 2009 16:03:13.000 00:15:21.992 32 121.605january 7, 2009 16:04:09.000 00:16:17.988 12 45.591january 7, 2009 16:04:37.000 00:16:45.986 5 17.593january 7, 2009 16:06:41.000 00:18:49.978 3 9.593january 7, 2009 16:07:41.000 00:19:49.975 6 21.592january 7, 2009 16:08:41.000 00:20:49.990 8 29.592january 7, 2009 16:10:17.000 00:22:25.984 3 9.593january 7, 2009 16:10:57.000 00:23:05.982 1 1.594january 7, 2009 16:12:13.000 00:24:21.977 3 9.593__________ __________ __________ __________ ____________________ __________ _____18 end of report text file section
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sleep scoring
C
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• power in up to 6 adjustable frequency ranges
• absolute and relative power values
• versatile scheduling of analysis, epoch lengths, overlap factor, windowing
POWER SPECTRUM ANALYSISEEG analyzer module withing ecgAUTO software
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From top: cortex, hippocampus, muscle, sleep score, active wake, quiet wake, REM sleep, SW sleep
• easily differentiate
between
four sleep stages
• calculate global sleep
scores
• FFT power spectrums
• epileptic seizures detection
• optional video for
confirmation
of sleep score
SLEEP SCORING
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Spectrum shown for each epoch - Automated and manual scores
SLEEP SCORING
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SLEEP SCORING MODULE
• define thresholds applicable to any parameter from any input
• software applies logical rules to attribute 1 out of 4 sleep stages to each epoch
• trend graph displays parameters as well as sleep stages
• on the fly, adjust thresholds and see the effect on sleep scoring
• reassign scores, also using up to 15 custom labelled scores
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SPECTRUM DATA EXPORT
Export FFT spectrum from each epoch
Possibility to group frequency data to limit number of data columns.
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rodentPACK2 telemetry
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TETHER-FREE EEG TELEMETRYrodentPACK2 wireless, low cost, head-mounted EEG for rodents
Acquisition of EEG and other biopotential
signals, as well as activity/ positional data
Applications are varied, e.g. sleep and
epilepsy studies, ECG and EMG recording
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• head-mounted biopotential telemetry for rodents- transmits data via radiotelemetry
• humane alternative to tethered EEG
• high signal to noise ratio
• replaceable battery for lifetime monitoring
TETHER-FREE EEG TELEMETRYrodentPACK2 wireless, low cost, head-mounted EEG for rodents
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• 4 biopotential signals (EEG, ECG, EMG)
each sampled at 1 kHz
• 3-axis acceleration, sampled at 50Hz on
each axis
• global acceleration (computed from 3
axes), for activity assessment
• 1 temperature, sampled at 1Hz
TETHER-FREE EEG TELEMETRYrodentPACK2 wireless, low cost, head-mounted EEG for rodents
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• electrodes implanted in the subject and linked to
a connector permanently glued onto the skull
• transmitter housing amplifiers, transmission electronics
and batteries, to be coupled to the head connector prior to experiment
• receiver to read transmitted data
TETHER-FREE EEG TELEMETRYrodentPACK2 wireless, low cost, head-mounted EEG for rodents
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FEATURES
Radio transmission range 5m or more
Transmission duration with same batteries: 150 hours
(cost of batteries: 3€ ($4US) / pair)
Group housing multiple animals in same cage
Up to 32 animals in same room with no required shielding
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ADVANTAGES
Noise free, high quality EEG signal
EEG electrodes are rigidly welded on the head connector which is itself solidly
linked to the transmitter, therefore animal movements trigger no flexible wire
movement and cause no artefact
3-axis acceleration allowing activity and posture assessment
Very low recurring cost
no transmitter refurbishment is ever necessary
batteries cost 3€ per recording
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MORE ADVANTAGES
Low investment cost
To record simultaneously from 4 rats, your complete hardware investment is less than 13k€ ($18kUS)
Even lower investment cost
By scheduling your recordings, you may further decrease cost.
For example, conducting a study on 16 subjects split in 4 groups of 4, only requires a total hardware investment of 14k€ ($20kUS)
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DATA TRACESfrom rodentPACK2
5s of data from a rat - cortex, hippocampus, EMG, total acceleration
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DATA TRACESfrom rodentPACK2
50s of data from a rat - cortex, hippocampus, EMG, total acceleration
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FREQUENTLY ASKED QUESTIONS
Does the device alter subject behaviour?
After an acclimation of under 24 hours, the rats ignore the device. They do not « touch » it nor do they try to rub it on the cage sides
How long can the implanted electrodes and glued connector stay in place ?
Rats equipped with this types of electrodes and connectors have been routinely used for many years in tethered systems
Rats used for our tests were implanted 12 months ago and are still in good health.
No special precautions were taken during this period to keep the skin-to-glue junction clean or sterile.
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third party data files
easyMATRIX to read telemetry implant signal
E
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ANALYZING DATA FROM THIRD-PARTY SYSTEMS
emka TECHNOLOGIES analysis software can process data in the following
formats:
• produced by emka iox acquisition software
• produced by DATAQUEST or PONEMAH software
• available as text files, for example from powerLab or Biopac
• available in format such as EDF, Ishne, others
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Invasive telemetry acquisition with easyMATRIX
A seamless solution to combine measurements from telemetry implants and the power of emka TECHNOLOGIES software suite
easyMATRIX
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• reads signal from any type of implant through any receiver
• contains its own ambient pressure transducer
• connects to computer through USB port (no need for an add-on A/D
acquisition card)
• also runs when 2 to 4 receivers read signal from a single implant
• iox2 directly records fully calibrated data
• OpenART not required
easyMATRIX
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• up to 16 receivers connect to a single easyMATRIX
• up to 8 analog signals connect to a single easyMATRIX
• perfect synchronization between telemetry implants and analog
signals
•1 easyMATRIX manages 1 to 4 receivers per implant
• up to 4 receivers connect to a single easyMATRIX
• up to 4 easyMATRIX connect to a single computer
easyMATRIX
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LEGACY & easyMATRIX SYSTEMS
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4 animals with 4 receivers/animal
SETUP EXAMPLESETUP EXAMPLE
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16 receivers connected to a single easyMATRIX
SETUP EXAMPLE
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