clostridia enrichment
DESCRIPTION
HMC 2012 Enrichments & Isolations Presentation. Clostridia Enrichment. Guangfei Liu. Clostridium (Kloster/ klwshr , Spindle). 梭 Shuttle. Endospores. - PowerPoint PPT PresentationTRANSCRIPT
Clostridia Enrichment
Guangfei Liu
HMC 2012 Enrichments & Isolations Presentation
Clostridium (Kloster/, Spindle)
梭 Shuttle
Endospores
Tortora et al. 2008. Microbiology An Introduction, 10th edition.
Clostridium is a genus of Gram-positive bacteria, belonging to the Firmicutes. They are obligate anaerobes capable of producing endospores
The Enrichment
The Enrichment
37 °C
The Enrichment
Pectins Utilization
Pectins are linear polysaccharides derived from galactouronic acid units, where some of the carboxylic acid groups are esterified with methanol. During fermentation, the ester groups are hydrolyzed, and methanol is released
OH
HO
OHOH
HOOC
O
Galactouronic acid Pectins
Methanol was the major nongaseous end product produced by some of the species examined. For C. buwricum strain 4P1 on pectin (mM): 16, methanol; 11.4, butyrate; 7.5, acetate; 2.3, ethanol; 0.8, lactate; and 0.7, isopropanol. The methanol produced by the species examined here was not further consumed. In control experiments, these species did not grow on methanol as energy source.
Inoculation and Incubation
80 °C
37 °C
+ CO2
Fermentation of glucose by Clostridia. Glucose is degraded to pyruvate via the Embden-Meyerhof pathway
Lengeler et al. (2009) Biology of the Prokaryotes
GYT Broth (g/L): 10.0 glucose; 5.0 tryptone; 10.0 yeast extract; 0.5 Na-thioglycolate; 20.0 CaCO3.
Glucose Butyrate- + 2CO2 + 2H2 + H+
ΔG°’ = -255 kJ/mol glucose (85 kJ/mol ATP)
Glucose + 2H2O 2Acetate-+ 2CO2 + 4H2 + 2H+
ΔG°’ = -206 kJ/mol glucose (52 kJ/mol ATP)
3 ATP
4 ATP
Glucose 0.7 Butyrate- +0.6 Acetate-+ 2CO2 + 2.6 H2
+ 1.3 H+
ΔG°’ = -240 kJ/mol glucose (71 kJ/mol ATP)
3.3 ATP
Acid Production and Catalase Test
Negative Positive
Propionic-acid-bacteriaClostridia
Thank you!