Clinostat Experiment The Scientific Writing Competition

For Student

KIBO-ABC WORKSHOP Bali, 30 November 2015

Rasdewita K. (rasdewita.kesumaningrum@lapan.go.id),

Space Science Center-LAPAN

The Scientific Writing Competition Clinostat Experiment

Theme: Space Simulation on Life Science In cooperation with the School of Life Sciences

& Technology, Institut Teknologi Bandung(SITH-ITB) and the National Institute of Aeronautics and Space (LAPAN).

Clinostat Using a Clinostat provided by LAPAN,

http://iss.jaxa.jp/en/kuoa/news/movie_1506/

A Clinostat is an experimental device that simulates microgravity environments

http://iss.jaxa.jp/en/kuoa/news/movie_1506/http://iss.jaxa.jp/en/kuoa/news/movie_1506/

Competition Process The five finalists were selected from among 28 proposals from

Indonesian high schools. Finalists proposals were selected by a panel of judges from

LAPAN and SITH-ITB, based on their experiments feasibility and the potential benefit of its results.

Each experiment were conducted by a team of three students with one assisting teacher.

The winners of the final competition was decided based on their experiments process and results.

These experiment ideas from Indonesias next generations are highly anticipated to one day grow into ideas for microgravity experiments in the Kibo module on the International Space Station, and to contribute to better utilization of the space environment by Indonesia and other Asia-Pacific countries. The competition is held as a part of Space Science Festival, an annual event organized by LAPAN to celebrate World Space Week (worldspaceweek.org).

The Proposal

Proposal title Students Microgravity effect on the metamorphosis of Tenebrio molitor Erma Kumala Dewi et.al. Trembesi seeding using clinostat Ridho Syifa Ezra Ibrahim et.al. Microgravity effect on the growth of green beans Aditya Negara et.al. Microgravity effect on the growth of chicken embryo Inggrid Dewi Rucita S et.al. Microgravity effect on Plant Stanol Ester level Ragil Pangestu et.al. Relation between microgravity and Auxin to the plants growth on the ground and space using clinostat Selly Febrilia Mayora et.al. The growth of green beans, red beans and peanuts in the microgravity condition Dliyaul Mushthafa et.al. Microgravity effect on the growth of green beans Muh Lucky Kurniawan Putra et.al. Comparison of green bean phototropisme rate data to microfravity effect using clinostat Fikry Iqbal Fadhillah Romadhan et.al. Lentils growth experiment in microgravity conditions using clinostat Nanda Faradiza Jolanet Adam et.al. microgravity effect of butterfly pupa growth Novianty Lubis et.al. microgravity effect of gene aux expression to Arabidopsis plant Daud Wahyu Imani et.al. Modification on the growth of sweet potatoes plants (Ipomoea batatas) to the effect of microgravity Muhammad Rasyid Ridho et.al. Microgravity effect on the growth of dragonfly Agra Dhanurwedha Sakti et.al. Microgravity effect to Giberelin hormone activity on seed germination of monocots and dicots Ihya Fakhrurizal Amin et.al. Farming in the environment affected by microgravity Rizky Amira et.al. Microgravity effect on the growth of paddy plant Agung Yoga Pangestu et.al. Autotomy and regenerastion of lizard on microgravity Dina Ainiyyah et.al. The effect of microgravity environtment simulation to the growth of Agaricus bisporus Ervin Naufal Arrasyid et.al. Microgravity effect to the movement pattern of spring and pendulum Erick Wicaksono Ramas et.al. Microgravity effect on the growth of Bryopsyda David Khowanto et.al. Microgravity effect on the growth of Capsicum frutescens L. and its fermentation distribution and also CO2 supply on photosynthesis process Lutfiah Amanda Harris et.al. The effect of microgravity and electromgnetic radiation to the growth of paddy plants Gigih Mahendra Putra et.al. effectivity of Rhizobium japonicum on Soya plants growth that treated by microgravity Maulana Nur Ikhsan et.al. Microgravity effect on the growth of Bryophyllum pinnatum Citra Faradina Fitriyanti et.al. Microgravity effect on the growth of living things David Sun Ong et.al. The effect of smoke on mammals plungs on microgravity Merci Fourte Yusuf et.al. Microgravity (Clinostat) effect on the growth of layered bulbs of onion Iqbal Fadhlurrahman et al

The Finalist

Experiment time : Juli October 2015 During experiment, progress report: 2

Team (School) Title

Erma Kumala Dewi et.al.(SMAN 3 Malang)

Microgravity Effect on the metamorphosis of mealworms (Tenebrio molitor)

Ervin Naufal Arrasyid et.al.(SMAN 1 Bandung)

The effect of microgravity environment simulation to the growth of Pleorotus ostreatus

Maulana Nur Ikhsan et.al. (SMAN 17 Makasar)

Efectivity of Rhizobium Japonicum on Soya plants growth that treated by microgravity

Gigih Mahendra Putra et.al.(MAN Sidoarjo)

The effect of microgravity and electromagnetic radiation to the growth of paddy plant

David Khowanto et.al. (SMAK Imanuel Pontianak)

Microgravity effect on the growth of mosses (Bryopsyda)

The Final Presentation

Microgravity Effect on the Metamorphosis of Mealworms (Tenebrio molitor)

Sumber: http://www.trubus-online.co.id/tru/ wp-content/uploads/2014/11/ulat-hongkong1.jpg

Metamorphosis is a biological process by which an animal

physically develops after birth or hatching, involving a

conspicuous and relatively abrupt change in the animal's

body structure through cell growth and differentiation.

Mealworms has complete metamorphosis stage

Mealworm is an object that relatively easy to find, adaptivable and usable as decomposer

Hypothesis: There is the effect of microgravity on metamorphosis time and morphology of mealworms (Tenebrio molitor)

http://www.trubus-online.co.id/tru/wp-content/uploads/2014/11/ulat-hongkong1.jpghttp://www.trubus-online.co.id/tru/wp-content/uploads/2014/11/ulat-hongkong1.jpg

31 days Observations The objects under microgravity experiment have metamorphosis time longer than those under normal condition This is thought because of the fisiological adaptation

of object on clinostat, that affect the stimulation or inhibition of certain metamorphosis hormones

The morphology difference between mealworms beetle under microgravity and normal condition is not significant, (1 of 12 is different, the object has problem on its motility (motion ability) , beetle movement seemed to drag.

The Effect of Microgravity Environment Simulation to the Growth of Pleorotus ostreatus

Kingdom : Fungi Divisi : Basidiomycota

Kelas : Agaricomycetes Ordo : Agaricales Famili : Pleurotaceae Genus : Pleurotus Spesies : P. ostreatus

Hypotesis 1. Gravity affect the growth and development

of Pleurotus ostreatus both in normal and almost zero gravity condition

2. Pleurotus ostreatus grows much faster under normal condition.

(the growth effect examined is on the morphology of Fungi)

Pleorotus ostreatus: widely cultivated mushroom with economic values in Indonesia

Results

Gravity and Microgravity environtment didnt affect the growth rate of Pleorotus ostreatus

There is a difference in morphology between Pleorotus ostreatus under normal condition and microgravity environment

The experiment variables: 1. independent

variable : gravity: 2. Controlled

variables: baglog quality, temperature, pH, water, nutriition, light, humidity.

3. Dependent variables: morphology differences of Pleurotus ostreatus under normal gravity and microgravity environment

Microgravity Effect on the Growth of Mosses (Bryopsyda)

Bryopsyda has important role on the ecosystem: Maintain soil moisture provide humus to the soil Indicator mineral deposits

Hypothesis: The microgravity doesnt affect the area covered by moss leaves

The microgravity affects the distribution of moss leaves

Results

Distribution area of spores of moss

under microgravity is more equitable and more widely than distribution area of moss spore

under normal condition

Moss leaves need long time period (about 6 monts) for its growth and development so it can be seen by naked eye Such a relatively short experiment

time span, the observations done by microscope, to examine the spread of spores of moss leaves that already have sporangium

Effectivity of Rhizobium Japonicum on Soya Plants Growth that Treated

Under Microgravity Condition

Soybean is a one of source of protein, that is important especially in the food resources.

soybean seed placed on clinostat

Soybean plants with root nodules

Object: Soybean seeds (Glycine max L.), pure isolates bacterium Rhizobium japonicum Rhizobium is shaped Gram-negative bacilli.

Hypothesis: Microgravity and inoculation of Rhizobium japonicum sp. affect the Soybean stem length and number of soybean leaves

Results

soybean stem length and number of leaves t under microgravity /and

inoculation of Rhizobium japonicum sp. are higher than to soybean stem length and number of leaves in normal gravity conditions /and inoculations Rhizobium japonicum.

The Effect Of Microgravity And Electromagnetic Radiation To The Growth Of

Paddy Plant

Hypothesis: the development and growth of rice plants is affected by

microgravity and electromagnetic wave radiation.

a) How is the development of the paddy plant cells to microgravity

environment and radiation of electromagnetic waves?

b) How does the growth of the paddy plant cells to microgravity environment

and radiation of electromagnetic waves?

Results A : rice plants treated with electromagnetic waves and under microgravity condition

B: rice plants under microgravity condition

C: rice plants under normal condition

Grow faster Grow slower Grow normal

Kemotropisme growth (the root moving towards the nutrients)

Kemotropisme growth (the root moving towards the nutrients)

Geotropisme growth (roots move down attracted by gravity)

Root Cell Microscopy results: the structure of the root cells look different from normal.

Root Cell Microscopy results: The structure of root cells look different and tend to be smaller.

Results Cells Root Microscope: Normal root cell structure.

Results Microscopy Stem Cells: Root cell structure does not different

Results Microscopy Stem Cells: Root cell structure does not different

Results Microscopy Stem Cells: Root cell structure does not different

The Winners

1. First Prize : Microgravity Effect on the metamorph of

mealworms (Tenebrio molitor), Team : Erma Kumala Dewi, Radya Kusuma Ardianto, Dinda Ayu

Ramadhani (SMAN 3 Malang). 2. Second Prize: The effect of microgravity environment

simulation to the growth of Pleorotus ostreatus, Team : Ervin Naufal Arrasyid, Jefani Marrosa, Muhammad Rizki Purnama (SMAN 1 Bandung),

3. Third Prize: Microgravity effect on the growth of mosses (Bryopsyda), Team : Hanna Astuti Puteri Wongkar, Manda Petrina, David Khowanto

(SMAK Immanuel Pontianak) 4. Finalist I : Efectivity of Rhizobium Japonicum on Soya

plants growth that treated by microgravity, Team : Maulana Nur Ikhsan, Muhammad Rifqi, Ulfa Larasanty (SMAN 17

Makassar) 5. Finalist II : The effect of microgravity and electromagnetic

radiation to the growth of paddy plant, Team: M. Asad Rosyadi, Gigih Mahendra Putra, Haydar Muhammad N.

(MAN Sidoarjo)

Pre-selection Proposal Try Zero G for Asian Youth

No Title

1 Sedimentation (fluid suspension) by Syahrul Robbiansyah Ramadhan (SMA Yadika 12, Depok)

2 The movement of gas balloon in zero-G condition by Muhamad Iqbal Sarvatra, Nadra Aprilia Taurita (SMAN 97 Jakarta)

3 Where will the car go? by Reyna Cesariyani Rahmadianti Rusuldi (SMAN 5 Surabaya)

4 Playing music in space by Lailatur Maghfiroh, Muhammad Ircham, and Nadia Ayu Laksmidewi (MAN Sidoarjo)

5 The effect of zero gravity environment to torque and collision of Gasing (by M. Arifin Nurdiansyah and Aditrya Maulana Z. MAN Sidoarjo)

6 Bouncing ball in zero gravity by Muhammad Fayd Haekal (SMAN 1 Sumatera Barat)

Clinostat ExperimentThe Scientific Writing Competition Clinostat Experiment ClinostatCompetition ProcessThe ProposalThe FinalistThe Final PresentationMicrogravity Effect on the Metamorphosis of Mealworms (Tenebrio molitor) 9The Effect of Microgravity Environment Simulation to the Growth of Pleorotus ostreatusResultsMicrogravity Effect on the Growth of Mosses (Bryopsyda) 13Effectivity of Rhizobium Japonicum on Soya Plants Growth that Treated Under Microgravity ConditionResultsThe Effect Of Microgravity And Electromagnetic Radiation To The Growth Of Paddy PlantResults The WinnersPre-selection Proposal Try Zero G for Asian Youth