Claude Dubois agr.

Director Product Development & Technical Services

IR4 Biopesticide Workshop, September 21th, 2016

• Manufactured in Canada by AEF.• Manufactured in Canada by AEF.• Organic acids based bactericide & Fungicide.• Berries & Fruits & Veggies

Suppressed bacteria growth and have bactericidal activity;

Antifungal effects on (powdery mildew)

Conidia germination;

Germ tube formation and elongation;

Appressorium formation;

Hyphae branching and haustoriumformation.

Strawberry trial

Incidence of Angular leaf spot

Means not followed by a common letter are significantly different (P=0.05) as determined by LSD protected test

Trial with large commercial operation in 2014. Treatment applied by grower with a boomsprayer (200 psi). Sprayed volume: 700 L/ha. Strawberry variety: Seascape

10 pints/acre

Powdery mildew on GerberaMean percentage of leaf area covered with PM

a

12 p/ac

10 p/ac

Independent trial conducted from May to June, 2015 in a designated research greenhouse at Kwantlen Polytechnic University (KPU), Langley, British Columbia, Canada .

Treatment applied 4 times, every 7 days on May 29, June 5, June 12 and June 19.

Nova applied 3 times every 10 days on Mai 29, June 8 and June 18.

Mean percentage of leaf area covered with powdery mildew rated on the Horsfall-Barrett visual scale of 0-11 and transformed to percentages. Mean of five plants per plot.

Means followed by the same letter are not significantly different according to Tukey’s HSD test at P=0.05.

aa

aab b b

15 p/ac

25

30

35

40

% Severity of powdery mildew on Jonagold(Cornell extension)

a

10 pints/acre

0

5

10

15

20

25

Control FungOUT Merivon

4 fl oz/acre

b

c

%

Column numbers followed by the same letter are not significantly different at P=0.05 asdetermined by Tukey contrasts for multiple comparisons

Biofungicide

• Active ingredients: Garlic extract

• Liquid formulation

• Rate: 1,8%; 7 – 9 L/ha

• Againts :

– Apple scab

– Powdery mildew

– Botrytis (preliminary)

C) Before treatment, the conidia are in chains and the mycelium develop abundantly on the leaf surface.

D) After treatment with Buran, the conidia and the mycelium are plasmolyzied and are drying out

© Université Laval

a

20%

25%

30%

35%Powdery Mildew Severity on berries at harvest (%) october 2, 2015

Column numbers followed by the same letter are not significantly different at P=0.05 asdetermined by LSD; Severity : Prob. > F = <,0001*

bb

bcbc

c

0%

5%

10%

15%

20%

Control Sirocco 1,4kg/ha

Sirocco 2,8kg/ha

Potassium Bicarbonate

5kg/ha

Buran 9l/ha + Sirocco

0,5kg/ha

Pristine commercial

rate

xxxx xxxx xxxx

Botrytis Severity on berries at harvest (%) october 2, 2015

a

15%

20%

25%

Column numbers followed by the same letter are not significantly different at P=0.05 asdetermined by LSD; Severity : Prob. > F = 0,0003*

b b b bb

0%

5%

10%

15%

Control Sirocco 1,4kg/ha

Sirocco 2,8kg/ha

Potassium Bicarbonate

5kg/ha

Buran 9l/ha + Sirocco

0,5kg/ha

Pristine commercial

rate

%

xxxxxxxx xxxx

Bioherbicide AEF-1201

•Pine oil extract•Approved in organic production•Non selective herbicide (activity on weed and seed)

Dissolves the waxy coating of plantsDissolves the waxy coating of plantsCauses dessication of seed

Electron microscope photos showing different rate (control, 2,5% and 10%)

Bioherbicide AEF-1201

Evaluation 28 DAT, cherry Lambert

Flamming ControlAEF 1201 (2x)

Bioessay with AEF-1201

Arion rufus species