citrus – clean plant network (ccpn- ) present and...
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CITRUS – CLEAN PLANT NETWORK (C-CPN)PRESENT AND FUTURE
Georgios Vidalakis
Tier 2 For Citrus-CPN
Davis, CAMay 11, 2010
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Citrus Centers of Excellence
Purpose:• To provide a safe mechanism of
introduction of citrus varieties into USA
• To maintain primary sources of disease tested & true to type citrus trees
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Hawaii to follow
So Far in the C-CPN1. FloridaDepartment of Plant Industry:• Citrus Germplasm Introduction Program• Bureau of Citrus Budwood Registration
• Citrus Clonal Protection Program – CCPP-University of California & Citrus Industry
• National Clonal Germplasm Repository for Citrus & Dates–USDA-APHIS
2. California
3. Arizona, Texas, Louisiana, & Alabama• Various schemes (University and State) of Budwood
Certification & Distribution Programs
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Funding• State Departments of Agriculture• Growers’ and Nurserymen’ Tax Box Boards• Universities
Collaborative Ventures
Regulatory Platform for Operation• State• Federal
NCPN
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Elements of Citrus Programs
•Introduction
•Disease diagnosis
•Pathogen elimination
•Maintenance of tree sources
•Citrus material distribution
QUARANTINE
QUARANTINE RELEASE
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Facilities & Equipment:•Updates are required
•Under increasing pest pressure
•New Technologies need to be incorporated
Personnel:•Experienced and close to retirement
•New hires for overlapping and training
•Sustainability, growth, and improved services
NCPN Support for Citrus Programs
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Introduction →
Quarantine Facilities - FL & CA
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Disease diagnosis →Quarantine Facilities - FL & CA
B. LaboratoryA. Bio-indexing
ELISA
sPAGE
PCR
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A. THERMALTHERAPY
→ and Pathogen Elimination B. SHOOT-TIP MICROGRAFTING
Quarantine Facilities - FL & CA
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Quarantine Facilities - FL & CAIntroduction, Disease Bio-Diagnosis, & Pathogen elimination
• Personnel is required to support the quarantine activities
• Upgrade of quarantine facilities and equipment since…..o Pressure from pests and diseases is constantly
increasing
o The only three (3) people in the USA holding the
USDA-APHIS-PPQ permits for Citrus Germplasm
introductions operate in these facilities
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Disease Diagnosis - New TechnologiesQ-PCR
Quantitative Real Time-PCRMOL-PCR
Multiplexed Oligonucleotide Ligation-PCR
si-RNA and deep sequencing technologies become increasingly affordable….
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Maintenance & Distribution of Citrus MaterialCA, TX, and FL
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Maintenance & Distribution of Citrus MaterialCA, TX, and FL
• Support the existing infrastructure with upgrades, personnel, and resources
• Support the CA, TX, & FL expansion and duplication their foundation collections
• Most importantly however….
NCPN should support AZ, LA, and AL to set up small foundation blocks for the varieties important for the local citriculture
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FundingState Departments of AgricultureGrowers’ and Nurserymen’ Tax Box BoardsUniversities
Collaborative Ventures
Regulatory Platform for OperationState and Federal
NCPN
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Facilitate and Enhance Existing National Interactions
• AZ & TX, are depended on CA and FL programs for primary citrus propagative material. Currently, AL is also processing germplasm in CA
• Citrus germplasm is readily exchanged among the programs (CA has aprx. 65 var. from FL and 10 from TX)
The biggest challenge lies ahead…
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The National PassportFor
Citrus Propagative Material
Uncharted waters…
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NCPN Support is Required for the National Passport Program
• Dedicated people
• Travel among the programs
• Exchange of blind samples
• Co-training in the full array of diagnostics and
therapy procedures
• Establishment of the regulatory foundation for:
-Placement of safeguards and checks
-Proper operation according to State and
Federal regulations
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Thank You