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In this guide:
UniProt (Universal Protein Resource): A comprehensive, high-quality and freely accessible resource of protein sequence and functional information
Sequence repositories
Genomic/Proteomic Research
Large-scale Small-scale
Sharing and using data:
Use/organise
the data
Analyse/re-
analyse the
data
Compare/integrate
with other data sets
Data source in UniProt:
Direct submissions
Gene name
Protein name
PRKN
BRCA1
DONSON
HedgehogDystrophin
Fidgetin
Keywords
Calcium
Antibiotic
DigestionSpecies
Mus musculus
Caenorhabditis elegans
Arabidopsis thaliana
GO term
Kinase
activity
oviposition
lysosome
Disease
Alzheimer
Down
syndrome
Malaria
ATG12
ATG12
ATG5
ATG5
ATG12
ATG5ATG16
ATG16
• Search for the gene names individually using the UniProtKB free text
search bar
• Use the advanced search to search by organism
Under the search bar on the
homepage
Use the retrieve
tool to search
for multiple
entries at a time
List your gene names
Select your parameters
29 entries match the
three gene names
3 entries are curator reviewed
26 entries have not been
reviewed by a curator
O94817
O94817
Q9H1Y0
Q9H1Y0
O94817
Q9H1Y0Q676U5
Q676U5
• Search UniProt using identifiers
• Find corresponding genes and protein entries
Use the ID mapping tool to convert UniProt
identifiers (accession numbers) to gene
names
Convert non-UniProt identifiers to UniProt
accession numbers and vice versa
Q9H1Y0
ATG12
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VPNRHEISPGHDGTWNDNQLQEMAQLRIKHQEEL? =
Use Peptide search tool to find entries containing sequence
Use the Peptide search
tool to search for entries
containing a specific
string of amino acids
Enter peptide
sequence
Specify the organism
ATG12
ATG12
ATG5
ATG5
ATG12
ATG5ATG16
ATG16
Find:
The function of each
component with
evidence source and
publications listed
Find:
Binary one-to-one
interaction and
complex data
Q9H1Y0
ATG12
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VPNRHEISPGHDGTWNDNQLQEMAQLRIKHQEEL? =
Use Blast and Align to find similar proteins within the same
species and in different species
Search for similar
sequences using the
BLAST tool.
You can search for full-
length protein
sequences or small
sections of protein
sequence
The identity column
shows how similar your
search query is to
individual BLAST hits
in the results table
BLAST hits can be filtered
and individual hits can be
selected for further
analysis or added to the
basket
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To indicate functional, structural and evolutionary relationships
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Clicking on the number of
proteins will display all
the protein records in
UniProtKB
Download all proteome
sequence data as a fasta
file for comparison with
raw sequence data
Search for specific diseases using the
diseases tab on the homepage
Phenotypes as a result of
single/double/multiple mutations
Phenotypes as a result of RNAi,
indicating developmental stage if
applicable
Provide name of mutant if
known
Indicate if phenotype is due to
mutations of several amino
acids
PIs: Alex Bateman, Alan Bridge, Cathy Wu
Key staff: Cecilia Arighi (Curation), Lionel Breuza (Curation), Elisabeth Coudert (Curation), Hongzhan Huang
(Development), Damien Lieberherr (Curation), Michele Magrane (Curation), Maria Martin (Development), Peter McGarvey
(Content), Darren Natale (Content), Sandra Orchard (Content), Ivo Pedruzzi (Curation), Sylvain Poux (Curation), Manuela
Pruess (Coordination), Shriya Raj (Coordination), Nicole Redaschi (Development)
Content / Curation: Lucila Aimo, Ghislaine Argoud-Puy, Andrea Auchincloss, Kristian Axelsen, Emmanuel Boutet, Emily
Bowler, Ramona Britto, Hema Bye-A-Jee, Cristina Casals-Casas, Anne Estreicher, Livia Famiglietti, Marc Feuermann,
John S. Garavelli, Penelope Garmiri, George Georghiou, Arnaud Gos, Nadine Gruaz, Emma Hatton-Ellis, Chantal Hulo,
Nevila Hyka-Nouspikel, Florence Jungo, Kati Laiho, Philippe Lemercier, Yvonne Lussi, Alistair MacDougall, Patrick
Masson, Anne Morgat, Sandrine Pilbout, Catherine Rivoire, Karen Ross, Christian Sigrist, Elena Speretta, Shyamala
Sundaram, Nidhi Tyagi, C. R. Vinayaka, Qinghua Wang, Kate Warner, Lai-Su Yeh, Rossana Zaru
Development: Shadab Ahmed, Emanuele Alpi, Leslie Arminski, Parit Bansal, Delphine Baratin, Teresa Batista Neto,
Jerven Bolleman, Borisas Bursteinas, Chuming Chen, Yongxing Chen, Beatrice Cuche, Edouard De Castro, Tunca Dogan,
Elisabeth Gasteiger, Sebastien Gehant, Leonardo Gonzales, Alexandr Ignatchenko, Giuseppe Insana, Rizwan Ishtiaq,
Vishal Joshi, Dushyanth Jyothi, Arnaud Kerhornou, Thierry Lombardot, Jie Luo, Mahdi Mahmoudy, Andrew Nightingale,
Joseph Onwubiko, Monica Pozzato, Sangya Pundir, Guoying Qi, Daniel Rice, Rabie Saidi, Edward Turner, Preethi
Vasudev, Yuqi Wang, Xavier Watkins, Hermann Zellner, Jian Zhang
European Bioinformatics Institute
(EMBL-EBI), Hinxton, Cambridge, UK
SIB Swiss Institute of Bioinformatics
(SIB), Geneva, Switzerland
UniProt Team
Protein Information Resource (PIR),
Washington DC and Delaware, USA
See the full list of upcoming webinars at
https://www.ebi.ac.uk/training/webinars
Please fill in the survey that launches after the webinar – thanks!