chlorogenic acid and coffee prevent hypoxia-induced retinal degeneration
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Chlorogenic Acid and Coffee Prevent Hypoxia-Induced Retinal Degeneration . Holim Jang , Hong Ryul Ahn , Hyoung Jo , Kyung-A Kim , Eun Ha Lee , Ki Won Lee , Sang Hoon Jung , and Chang Y. Lee . The Retina. Photographic film of the eye Retinal ganglion (RGC-5) cells - PowerPoint PPT PresentationTRANSCRIPT
CHLOROGENIC ACID AND COFFEE PREVENT HYPOXIA-INDUCED RETINAL DEGENERATION
Holim Jang, Hong Ryul Ahn, Hyoung Jo, Kyung-A Kim, Eun Ha Lee, Ki Won Lee, Sang Hoon Jung, and Chang Y. Lee
The Retina Photographic film of the eye Retinal ganglion (RGC-5) cells
Axons form optic nerve
Hypoxia Metabolically active
Oxidative stress Reduction in oxygen supply
Risk factor for sight-threatening disorders
Nitric Oxide (NO) Hypoxia NO binds to Complex IV O2
- formed
Formation of peroxynitrite (oxidizing agent) NO + O2
- ONO2-
Chlorogenic Acid (CGA) Antioxidant phytochemical Major source is coffee High blood absorption rate (33%)
Green coffee
Question Do CGA and coffee protect against
retinal degeneration? In vitro & in vivo
Method: In Vivo Male mice 30-35 kg Optic nerve crush
Left: crush Right: control
CGA & coffee extract (oral)
Method: In Vivo RGC labeling: superior colliculi exposed
Fluorogold Eyeballs enucleated after 5 days Retinal flat mounts
Method: Analysis Hematoxilin & Eosin
staining Nuclei = blue Cytoplasm & proteins
= pink TUNEL staining
Apoptosis = brown
Results: CGA
Results: CGA
Results: CGA
Results: CGA
Results: Coffee Extract
Results: Coffee Extract
Results: Drawing Conclusions CGA’s NO scavenging activity
Optic nerve damage inflammatory response NO release
Supported by in vitro
Discussion Retinal degeneration from hypoxia CGA = NO scavenger Limitations
Mice CGA concentration