“Cherry Picker”

Blake Hondl, Amit Mehta,

Jon Millin, Ryan Pope

Advisor: Professor Tompkins

BME 301 Spring 2004

University of Wisconsin - MadisonBiomedical Engineering Design Courses

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Client

Noël R. Peters, M.S. Keck-UWCCC Small Molecule Screening

Facility

Overview

Problem Statement Background Design Constraints Design Alternatives Proposed Design Future Work

Problem Statement

A device to guide a micropipette user in the transfer of small volumes of compounds between 384-well micro liter plates. The device will increase the efficiency of transfers and reduce user error.

384-Well Plate

Background

Drug-like chemicals used in biological assays

Substance tested against 36,000 known chemicals

Chemical mixed with reagents in micro-liter wells

Biomek® FX Laboratory Workstation

Background

Well plates loaded into plate reader

Typically 1-4 wells meet the absorbance, fluorescence or luminescence requirements, known as “hits”

“Hits” are retested EnVison® Plate Reader

Design Constraints

Must interface with Microsoft Excel

Effectively guide the user to the appropriate wells

Minimize required bench space Withstand exposure to various chemicals

and reagents Lightweight and inexpensive (<$1000)

Current Competition

Matrix Memowell®

– 96 well pipetting aid– Lights up 4 wells when

used with 384-well plate– Costs approx. $1000

Quadra Cherry Picker– Automated– Costs $150,000

http://www.matrixtechcorp.com

http://www.tomtec.com

Computer Interface

Client uses a Dell PC with Windows® XP

Use a port to connect device to PC

– Monitor (VGA) port– USB or serial port

Send data through port to device

http://www.dell.com

Software

Choose a programming language– Java– C++

Choose a programming environment and compiler to create application

– Microsoft® Visual®

– Metrowerks™ CodeWarrior™

Application will take input from user and Excel file

Proposed Designs

384 Fiber Optic Array384 LED ArrayLCD Screen

Fiber Optics

384 individually controlled fibers

Low intensity light Very small light source

www.fiberopticproducts.com

384 LED Array

Similar to Memowell device Microcontroller interfaced with a Computer 384 surface mount LED’s Light up both row and column High intensity light source

http://www.globalspec.com

LED Schematic

Proposed Solution

LCD screen to show output of application

– 2 wells fixed in place on screen

– Lines (cross-hairs) displayed to identify wells to user

Application– Takes input from both an

Excel file listing “hits” and user

Picture of 384 well plate on lcd screenof a laptop.

Decision Matrix

  Fiber Optic LED LCD

Feasibility 3 2 1

Cost 2 2 3

Ease of use 3 2 1

Manufacturing (labor) 3 3 1

Adaptability 3 3 1

       

Average score 2.8 2.4 1.4

  Good Better Best

Future Work

Connect LCD screen through external port Determine best platform for implementation Experiment with output to LCD screen Determine necessary output for every well Write code to illuminate proper wells for all

possible combinations Construct user interface

Any Questions?

References

“LCD Basics: Monitor Technology 101.” ViewSonic. http://www.viewsonic.com/monitoruniversity/lcdbasics.htm

“LCD Video Controller.” Subassembly Product Guide. http://www.trans2000.com/manual/adboard_manual.pdf

“Product Specification for LB104V03 Liquid Crystal Display.” Products Engineering Dept. LG. Philips LCD Co., Ltd. http://www.jacoflatpanels.com/lcdpdfs/LB104V03-A1_CAS(Ver0.1).pdf

“Java Excel API Tutorial”.http://www.andykhan.com/jexcelapi/tutorial.html

Conclusion: LCD Device

Because of labor savings over soldering 768 individual LED’s (384 wells per plate times 2 plates) and because of the rapid adaptability of an LCD by the implementation of new programming, it was decided to pursue the LCD screen design further.

LCD Design

LCD contains:– One or more cold cathode fluorescent lamps

(CCFL)– Liquid crystal grid– Controlling circuitry

Video receiver circuit LCD driver circuit Inverter

System Diagram

ComputerUserApplication

Device

Typical LCD Schematic