chemical processes and separation technology on a ......2014/09/04 · 2 outline • company...
TRANSCRIPT
Joint gathering of NL GUTS & PIN-NL, 9 April 2014
- Confidential -
Mark Olde Riekerink
Chemical processes and separation technology on a microscale
2
Outline
• Company introduction
• Chemical processes on a microscale
• Continuous flow
• Multistack reactor
• Mesoreactor
• Microreactor
• Separation technologies on a microscale
• Capillary electrophoresis (CE)
• Dielectrophoresis (DEP)
• Acoustophoresis
• Passive microfluidics
confidential
3 - Confidential -
Micronit Microfluidics – Key Facts
Started in 1999 by Ronny van’t Oever & Micha Mulder (Founders)
Location : Enschede, The Netherlands
University of Twente: Hot spot of micro- & nanotechnology
Annual turnover growth > 40%
> 700 customers in > 50 countries worldwide
About 50 employees and growing
Highly educated multi-disciplinary team
ISO 9001 certified, ISO 13485 (in 2014)
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We serve
leading brands in
analytic instrumentation and
diagnostics globally
Our people serve
world’s top 10 technical universities
- Confidential -
International Customer Base
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MESA+, University of Twente
• One of the largest micro-/nanotech groups worldwide
• 500 people, 275 PhD’s and postdocs
2010 Nanolab
• Infrastructure : >60 million euros
• 1250 m2 cleanroom
2012 High Tech Factory (HTF)
• Production facility for micro- and nanotech-based
companies/products
• 13 cleanrooms, 20 laboratories, 46 offices
Enschede – Nanotechnology
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Micronit Cleanroom Facilities (HTF)
• Photolithography processing and
Microstructuring of glass wafers
- Confidential -
7 - Confidential -
Micronit – What We Can Offer
• Developing, prototyping and high-volume
manufacturing of standard & custom Lab-on-a-Chip
products
• Microfluidic solutions for Life Sciences,
Chemistry and Industrial applications
• Unique combination of microfluidics know-how,
semi-conductor, MEMS & micromachining
capabilities
• Market innovator powered by R&D department,
cleanroom facilities and intellectual property
8 - Confidential -
Core Business
MANUFACTURING
• High-accuracy micromachining
• Rapid prototyping
• High-volume manufacturing capabilities
DEVELOPMENT PARTNER
• Co-engineering
• Design for manufacturing
• Microfluidics know-how
RESEARCH TOOLS
• Easy-to-use microfluidic kits to get started
• Off-the-shelf standard microfluidic chip modules
• Custom prototyping & low-volume manufacturing
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Broad Range of Microfluidic Solutions
Droplet Generator Chips
Micromixers
CE Chips
Micro Reactor Chips
Flow Cells
Chip holders and connectors
Fluidic PRO Prototyping
Electrical connections
Macro-to-Micro interfacing
System integration and packaging
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Parallel droplet generation
- Confidential -
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Advantages of Glass (I)
CHEMICAL INERTNESS
• Solvents and water do not dissolve glass
• Minimum interference with reaction or analysis
THERMAL STABILITY
• Very well defined flat surface and mechanically stable
• Glass is stable in time and has a very long shelf life
OPTICALLY CLEAR
• Glass is optically superior, special types even in UV-range
• Glass has a low (auto) fluorescence
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Advantages of Glass (II)
KNOWN MATERIAL
• Glass is relatively easy to coat using traditional procedures
• Easier to comply with regulations
NON-POROUS
• Small molecules won’t diffuse into glass
• No materials can diffuse out of the glass
SUITABLE FOR LOW- AND HIGH-VOLUME MANUFACTURING
• Micromachining in glass in volume is very economical
• Prototyping processes identical to manufacturing processes
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Outline
• Company introduction
• Chemical processes on a microscale
• Continuous flow
• Multistack reactor
• Mesoreactor
• Microreactor
• Separation technologies on a microscale
• Capillary electrophoresis (CE)
• Dielectrophoresis (DEP)
• Acoustophoresis
• Passive microfluidics
confidential
14
Development of continuous flow systems
confidential
Partnership
Access2Flow:
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Chemical processes down to microscale
Laboratory Production Plant
Nano-Microliter
• fast and cost effective
screening
1-10 ml
• optimization via
modular approach
10-100 ml
• production in
parallel reactors
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Multistack reactor (DSM)
• 22 glass layers, of which 10 x 10 ml reaction channels
• 11 cooling layers
Developed for and in
cooperation with DSM
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Mesoreactor
• Reactor volume 3 ml
• 4 bonded glass layers
• Highly efficient mixing structures
- Confidential -
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FLOWID Plug & Produce Flow Chemistry
- Confidential -
Switch from batch to continuous flow
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Microreactor Platform - Fluidic Connect Pro
Standard fluidic devices: (75x25mm)
mixers, reactors, droplet generators, CE
Reactor volume ~10 µl
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FlowStart Microreactor System
• Smart, safe and clean chemistry
• Accelerates the optimization of chemical processes
by automated microreactor technology
• Batch-to-flow translations
The FlowStart microreactor system,
developed in cooperation with Micronit
Microfluidics
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Outline
• Company introduction
• Chemical processes on a microscale
• Continuous flow
• Multistack reactor
• Mesoreactor
• Microreactor
• Separation technologies on a microscale
• Capillary electrophoresis (CE)
• Dielectrophoresis (DEP)
• Acoustophoresis
• Passive microfluidics
confidential
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Capillary electrophoresis (CE)
• Separation of charged molecules (e.g. ions, DNA fragments) based on
their charge and mobility
• Separation accuracy (resolution) dependent on channel length
• Microfluidic applications:
• DNA analysis
• Blood analysis
• Water analysis
confidential
23 - Confidential -
CE for Point-of-Care Applications (Lab-on-a-Chip)
Features Point-of-Care Lithium Chip
• Glass chip with etched channels
• Powderblasted access holes and reservoirs
• Sputtered metal electrodes
• Dry dicing technique
• 1 cm2 surface area
• CE marked
• On-chip reagents
• Plastic packaging
• Manufacturability in high volume
• Quality assured
• Economic pricing (one-time used chip)
• > 100.000 devices/year
Source: www.medimate.com
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CE for Automated DNA Separation (Fingerprinting)
Sources:
www.chem.agilent.com
www.bio-rad.com
www.perkinelmer.com
www.shimadzu.com
Chip-based capillary gel electrophoresis is
a proven technology for e.g. DNA analysis
Forensics
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CE System for DNA Fingerprinting (conceptual)
- Confidential -
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CE Lab-on-a-Chip Prototypes
- Confidential -
Cartridge format
USB stick format
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Dielectrophoresis (DEP) for cell manipulation/sorting
- Confidential -
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Acoustophoresis for separation/concentration
• Ultrasonic standing waves allow high-performance separation/
concentration of cells or particles
confidential
Source: www.cellcare.lth.se
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Microfluidic chip for cell separation/concentration
Yeast separation Blood cell separation from plasma