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PURDUE UNIVERSITY

Chemical Hygiene Plan and Hazardous Materials Safety Manual

Laboratory Specific Plan

This is the Chemical Hygiene Plan specific to the following areas:

Building(s): BRWN

Room Number(s): 3154, 5106, 5185

Principal Investigator (Supervisor): Patricia Bishop

Department:

Revised (Must be reviewed at least annually.): September 12, 2014

Important Telephone Numbers:

1. 911 for All Emergencies

2. (765) 49‐48221 Purdue Police Department (Non‐Emergency Line)

3. (765) 49‐46919 Purdue Fire Department (Non‐Emergency Line)

4. (765) 49‐46371 Purdue REM (Do Not Use for an Emergency)

All laboratory chemical use areas must maintain a work‐area specific Chemical Hygiene Plan which conforms to

the requirements of the OSHA Occupational Exposure to Hazardous Chemicals in Laboratories (29 CFR 1910.1450).

Purdue University laboratories may use this document as a starting point for creating their work area specific

Chemical Hygiene Plan. Minimally, this cover page is to be edited for work area specificity (non‐West Lafayette

laboratories are to place their own emergency, fire, and police telephone numbers in the space above) and the

Purdue Chemical Hygiene Plan Awareness Certification Form must be completed for all lab employees. This

instruction and information box should remain. This model Chemical Hygiene Plan is the 2014 version; the most

current version can be found on the Forms page at www.purdue.edu/rem.

Department of Chemistry

CHP DocChapter 1.1 P1.2 Sc1.3 C1.4 E1.4.11.4.21.4.31.4.41.4.5

1.5 R1.6 In1.7 C

Chapter 2.1 G2.1.12.1.2

2.2 N2.3 D

Chapter 3.1 P3.2 H3.3 B3.4 R3.5 La

Chapter 4.1 E4.1.14.1.24.1.34.1.44.1.54.1.64.1.7

4.2 A4.2.14.2.2

4.3 P

ument Acro Introduc1:

urpose .......cope ...........HP Use Instrmployee Rig Laborato1 Laborato2 Laborato3 Non‐Lab4 Chemica5adiological &ntegrated Sahemical and Chemica2:

Globally Harm1 Safety Da2 ChemicaNational Fire Department o

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Health Hazardiological Hazadioactive Maser Hazards Laborato4:

ngineering C1 Chemica2 Glove Bo3 Laminar 4 Biologica5 Safety Sh6 Fire Extin7 Fire DooAdministrativ Standard1 Required2ersonal Prot

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onyms List ...ction .................................................ructions ......ghts and Resory Supervisoory Employeory Safety Oforatory Persl Hygiene Of& Environmeafety Plan ....d Laboratoryal Classificatmonized Systata Sheets ..l Labeling ...Protection Aof Transportof Hazardouards .............ds ...............zards ..........Material Hazs .................ory Safety CoControls andl Fume Hoodoxes ............Flow Clean al Safety Cabhowers and nguishers ...rs ...............ve Controls .d Operating d Laboratorytective Equip


Table o

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..............................................................................sponsibilitiesor Responsibe Responsibfficer Resposonnel / Supfficer Respoental Manag....................y Safety Comion Systemstem for Clas........................................Association tation Hazars Chemicals............................................................zards ................................ontrols .......d Safety Equids ....................................Benches ......binets ..........Eyewash Sta............................................................Procedures .y Postings ....pment (PPE)

i

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..............................................................................s ..................bilities .........bilities ..........nsibilities ....pport Staff Rnsibilities ....gement Depa....................

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Table of C

...................

....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................

Contents

...... 1

...... 2 ...... 2 ...... 2 ...... 3 ...... 3 ...... 4 ...... 5 ...... 5 ...... 6 ...... 6 ...... 7 ...... 7 ...... 8 ...... 9 ...... 9 ...... 9 .... 10 .... 13 .... 14 .... 16 .... 16 .... 17 .... 18 .... 18 .... 18 .... 19 .... 19 .... 19 .... 22 .... 23 .... 23 .... 24 .... 25 .... 25 .... 26 .... 26 .... 27 .... 28

Chapter 5.1 La5.1.15.1.2

5.2 H5.3 C5.4 Sa5.5 C5.6 C5.7 C5.7.15.7.25.7.35.7.45.7.55.7.65.7.75.7.8

5.8 C5.9 C5.10 N5.11 S5.12 E5.125.125.125.125.125.125.12

5.13 R5.14 T5.145.145.14

5.15 La5.16 La5.17 La5.175.175.175.17

Laborato5:aboratory Sa1 Laborato2 General Housekeepinhemical Inveafety Data Shemical Labhemical Seghemical Sto1 General 2 Flammab3 Compres4 Reactive5 Acutely T6 Corrosive7 Oxidizers8 Refrigeraompressed ryogenic Liq

Nanoparticle harps Handlquipment, A.1 Centrifug.2 Stirring a.3 Heating .4 Distillatio.5 Laborato.6 High Pre.7 Vacuum esearch Samransporting 4.1 Shipping4.2 Transpor4.3 Transporaboratory Seaboratory Eraboratory El

Training .1 Portable.2 Tempora.3 Wet or D.4

The officia

ory Manageafety Guidelory Safety QuLaboratory Sg ................entories .....Sheets .........beling Requirgregation ....rage RequireChemical Stble Liquids Sssed Gases S Materials SToxic Materie Materials Ss and Organators and FreGas Cylinderquids Safety Safety ........ing Safety ..Apparatus, ages .............and Mixing EDevices ......on and Solveory Glasswarssure SystemSystems .....

mples and ChHazardous C

g Hazardous rting Chemicrting Chemicelf‐Inspectiorgonomics ..lectrical Safe................... Electrical Eqary Wiring RDamp Locatio


ment Plan ..ines .............uestions ......Safety Rules............................................................rements ..........................ements........orage ..........Storage ........Storage ........torage .........ials Storage .Storage .......ic Peroxide Seezers Chemr Safety ...................................................................nd Instrume....................Equipment .......................ent Purificatre .................ms ...................................hemicals DevChemicals ...Chemicals ocals on Campcals on Campons ...................................ety ...................................quipment anequirementons ..............

ii

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Table of C

.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................

Contents

.... 29 .... 29 .... 29 .... 30 .... 31 .... 32 .... 32 .... 32 .... 33 .... 34 .... 34 .... 35 .... 36 .... 37 .... 38 .... 39 .... 39 .... 40 .... 41 .... 43 .... 44 .... 44 .... 45 .... 45 .... 46 .... 46 .... 47 .... 48 .... 48 .... 49 .... 50 .... 51 .... 52 .... 52 .... 54 .... 55 .... 56 .... 56 .... 56 .... 56 .... 58 .... 59

Chapter 6.1 P6.2 Sc6.3 H6.3.16.3.26.3.3

6.4 M6.4.16.4.26.4.36.4.46.4.56.4.66.4.7

6.5 M6.6 P6.7 In

Chapter 7.1 In7.2 W7.3 W7.4 W7.5 W7.6 U7.6.17.6.27.6.37.6.4

7.7 S7.8 S7.9 Li

Chapter 8.1 N8.2 E8.3 C

Chapter 9.1 C9.1.1

9.2 P9.3 SO9.4 La9.5 R

Appendix

Laborato6:urpose .......cope ...........Hazard Asses1 Task Eva2 Location3 Job Title Minimum PP1 Head Pro2 Hearing 3 Respirato4 Eye and 5 Hand Pro6 Body Pro7 Foot ProMinimum PPPE Training njuries, Illnes

Hazardo7:ntroduction Waste IdentifWaste StoragWaste ContaiWaste DisposUnknown Che1 Labeling 2 Identifyi3 Removin4 Preventiink and Trasharps Wasteiquid Chrom Chemica8:

Non‐Emergenmergency Chemical Spil Training 9:

HP Training 1 Annual CPE Training OP Training aboratory ChEM Researcx A: CHP Tr

The officia

ory PPE Polic......................................sment ........luation Haza Evaluation Evaluation HE Requiremeotection ......Protection .ory ProtectioFace Protectotection .....otection ......tection .......E RequiremeRequiremensses, and Meus Waste M...................fication and ge Requireminers ...........sal Proceduremical WastUnknown Cng Unknownng Unknown ng Unknownsh Disposal ..e .................matography Wal Spills .......ncy Chemicahemical Spilll Kits ...............................................CHP Refreshe......................................hemical Safeher’s Guide raining Certi


cy ..........................................................................ard AssessmHazard AsseHazard Asseents for Labo........................................on ...............tion .........................................................................ents for Supnts ................edical Exam

Management....................Labeling .....ents ................................res ...............te .................Chemicals .....n Chemicals .Chemicals fn Chemicals.........................................Waste ............................al Spill Procel Procedures..........................................................er Requirem........................................ety Course (C....................ification For

iii

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..............................................................................

ment .............essment .......essment .......oratories .................................................................................................................................................port Staff an....................inations ......t .................................................................................................................................................................................from the Wo..................................................................................................dures ..........s ............................................................................

ments ...................................................CHM 605) .......................rm ..............

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Table of C

...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................

Contents

.... 60 .... 60 .... 60 .... 60 .... 61 .... 61 .... 62 .... 62 .... 63 .... 63 .... 64 .... 64 .... 64 .... 65 .... 66 .... 66 .... 66 .... 67 .... 69 .... 69 .... 70 .... 71 .... 72 .... 74 .... 75 .... 75 .... 75 .... 76 .... 76 .... 77 .... 77 .... 77 .... 79 .... 79 .... 79 .... 80 .... 81 .... 81 .... 81 .... 82 .... 82 .... 83 .... 83 .... 84

AppendixB.1 PB.1.1B.1.2B.1.3B.1.4B.1.5B.1.6B.1.7B.1.8

B.2 HB.2.1B.2.2B.2.3B.2.4B.2.5

BBB

AppendixTab 1: STab 2: LTab 3: L

x B: OSHA hysical Haza Flammab1 Flammab2 Gases un3 Pyropho4 Water‐R5 Oxidizers6 Organic 7 Explosive8

Health Hazard Irritants 1 Sensitize2 Corrosive3 Hazardo4 Particula5.2.5.1 Car.2.5.2 Rep.2.5.3 Subx C: PeroxiSpecific StanLab‐Specific Lab‐Specific

The officia

Hazard Clasards .............ble Liquids ..ble Solids ....nder Pressurric, Self‐Heaeactive Mats .................Peroxides ...es ...............ds ..................................ers ...............es ...............us Substancarly Hazardorcinogens ....productive Tbstances witde Forming ndard OperaProtocols, RHazard Asse


ss Definition............................................................re .................ating, and Seerials ......................................................................................................................................................es with Toxius Substanc....................Toxins ...........h a High AcuChemicals .

ating ProcedRequiremenessments....

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.......................................................................................................................................................................................................................................................................................gans .........................................................................................................................................................................

Table of C

.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................

Contents

.... 86 .... 87 .... 87 .... 87 .... 88 .... 88 .... 89 .... 89 .... 89 .... 90 .... 90 .... 90 .... 90 .... 91 .... 91 .... 91 .... 92 .... 92 .... 93 .... 94 .... 97 .... 98 .... 99

CHP Document Acronyms List

1


CHP Document Acronyms List

ANSI American National Standards Institute

ASTM American Society of Testing and Materials

CFR Code of Federal Regulations

CHO Chemical Hygiene Officer

CHP Chemical Hygiene Plan

CLSC Chemical and Laboratory Safety Committee

DOT Department of Transportation

EHS Environmental Health and Safety

EPA Environmental Protection Agency

GFCI Ground Fault Circuit Interrupter

GHS Globally Harmonized System of Classification and Labeling of Chemicals

HBr Hydrogen Bromide

HF Hydrofluoric Acid

HEPA High‐Efficiency Particulate Air

HPLC High Performance Liquid Chromatography

IBC Institutional Biosafety Committee

IDEM Indiana Department of Environmental Management

ISP Integrated Safety Plan

LC Liquid Chromatography

LC50 Lethal Concentration 50%

LD50 Lethal Dose 50%

LEL Lower Explosive Limit

LSC Laser Safety Committee

MSDS Material Safety Data Sheet

NFPA National Fire Protection Association

OSHA Occupational Safety and Health Administration

PCB Polychlorinated Biphenyl

PHS Particularly Hazardous Substance

PI Principal Investigator

PPE Personal Protective Equipment

RCRA Resource Conservation and Recovery Act

rDNA Recombinant Deoxyribonucleic Acid

REM Radiological and Environmental Management

RSC Radiation Safety Committee

SAA Satellite Accumulation Area

SDS Safety Data Sheet

SOP Standard Operating Procedure

UEL Upper Explosive limit

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Chapter 1: Introduction

3


4. Protective laboratory practices and equipment are made available and in common use

to minimize the potential for employee exposure to hazardous chemicals.

The CHP was prepared in accordance with the requirements of the Occupational Safety and

Health Administration (OSHA) Occupational Exposure to Hazardous Chemicals in Laboratories

Standard (Lab Standard) found in 29 CFR 1910.1450, and is based on best practices identified in,

among other sources, the “Global Harmonized System of Classification and Labeling of

Chemicals”; “Prudent Practices for Handling Hazardous Chemicals in Laboratories”, published

by the National Research Council, the American Chemistry Society Task Force on Laboratory

Chemical and Waste Management’s “Laboratory Waste Management, A Guidebook”; the

Princeton University “Laboratory Safety Manual”; and the University of California – Los Angeles

“Chemical Hygiene Plan”.

1.3 CHP Use Instructions

The information presented in the CHP represents best practices and provides a broad overview

of the information necessary for the safe operation of laboratories that utilize hazardous

chemicals. It is not intended to be all inclusive. Departments engaged in work with hazardous

chemicals or hazardous operations that are not sufficiently covered by the CHP must customize

this document by adding appropriate sections, in the form of standard operating procedures

(SOPs), hazard assessments, and any other written lab‐specific operating procedures that

address the hazards and how to mitigate risks. The following instructions detail how this CHP

template should be used and customized by each laboratory:

Review this template CHP provided by REM.

Insert your lab‐specific standard operating procedures (SOPs) into your customized CHP

under Tab 1 located in the back of the CHP document. More details regarding SOPs can

be found in Chapter 4 of the CHP.

Insert all other documented lab‐specific rules, requirements, and procedures (e.g.,

equipment protocols, internal lab inspections, etc.,) under Tab 2.

Insert your lab‐specific hazard assessments under Tab 3. More details regarding hazard

assessments can be found in Chapter 6 of the CHP.

Review, update (if necessary), and retrain all employees on the lab‐specific CHP at least

annually.

1.4 Employee Rights and Responsibilities

As part of the OSHA Laboratory Standard, employees and other personnel who work in

laboratories have the right to be informed about the potential hazards of the chemicals in their

work are

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nd PPE;

PE;

nd unsafe co

tory safety t

ance progra

odifications

ry, or chemic

rvisor (PI) to

es are being

PI establish

rogram. The

y operations

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aboratory Su

al (e.g., Labo

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t CHP trainin

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processes in

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involving sa

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Chapter 1: Intro

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Appendix A fo

ng. Failure to

om regulato

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e, or store

OPs required

ng work are

the laborato

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the laborato

provide writ

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a physician

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afety and

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ty

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1.4.4

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how the lab

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Chemical Hyg

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operates an

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afety Officer

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aboratory Sa

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back to the l

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maintenance

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Management

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must:


azardous wa

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lab manager

as:

w who to co

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g and equip

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a laboratory

quired or if o

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fficer Respon

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t Departmen

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6

ocument will only

ste procedu

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r). Having a

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PI to enforce

ective acade

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afety commi

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ment maint

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are safety g

other unique

notify suppo

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ab safety ex

Laboratory S

questions ab

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safety comm

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ude:

ppropriate tr

ittee meetin

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Responsibilit

ten must ent

enance. Sup

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glasses, long

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ort staff.

f the Purdue

ated individ

component

nline.

his person sh

xperience (e.

Safety Office

out daily op

rules;

ment; idea i

mittee meeti

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ties

ter laborato

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Minimum PPE

pants, and c

uirements m

e Radiologica

ual(s), has th

ts of the CHP

Chapter 1: Intro

hould be fam

.g., senior

er in each la

perations

s that each

ings and rep

to be involv

ven and that

ort significan

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members are

E requireme

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P. The Chem

oduction

miliar

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ved in

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nt

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mical


7


Inform Laboratory Supervisors of all health and safety requirements and assist with

the selection of appropriate safety controls (engineering controls, administrative

controls, and PPE);

Ensure that Laboratory Supervisors have the necessary resources to maintain

compliance with the CHP and that all lab staff receive appropriate training;

Act as the liaison between the Laboratory Supervisors and the Chemical Laboratory

Safety Committee;

Conduct periodic lab inspections and immediately take steps to abate hazards that

may pose a risk to life or safety upon discovery of such hazards;

Ensure that SOPs and hazard assessments are being prepared;

Maintain employee exposure‐monitoring records, when applicable;

Help to develop and implement appropriate environmental health and safety

policies and procedures;

Review and evaluate the effectiveness of the CHP program at least annually and

update it as appropriate; and

Actively enforce all applicable safety procedures and ensure the contents of the CHP

are followed; take appropriate actions when safety procedures are not followed.

1.5 Radiological & Environmental Management Department

The Radiological and Environmental Management Department (REM) serves as the

environmental health and safety department for Purdue University. REM’s primary role is to

manage regulatory compliance with all federal, state, and Purdue regulations involving

environmental health and safety issues. REM facilitates a number of programs that apply to

laboratory safety, a few of which include biological safety, laser safety, personal protective

equipment program, radiation safety, development of standard operating procedures, as well

as the CHP. REM also performs numerous safety inspections of facilities throughout the year to

monitor compliance with regulatory requirements. REM provides a variety of services such as

training, chemical, biological, and radioactive waste pickups, and safety consultation. More

detailed information regarding all of REM’s resources and services can be found on the REM

website. (http://www.purdue.edu/rem/)

1.6 Integrated Safety Plan

It is the policy of Purdue University to integrate environmental health and safety into all

operations. The Integrated Safety Plan (ISP) is Purdue University’s safety program, which is

facilitated by REM, and was developed to provide a framework for laboratories to comply with

environmental health and safety (EHS) regulations. The ISP assists in communication of EHS


8


issues across the organization and calls for departmental level safety committees and individual

self‐audits. The ISP provides indemnification from regulatory fines for units with a certified

safety program. An ISP certified safety program must have the following elements:

Regular safety committee meetings;

Means of communicating safety issues to the department in a timely manner;

Upper administrative support for safety;

Self‐audits checklists, which is a self‐inspection program, must be completed for all

areas;

Abatement of deficiencies found during the self‐audits;

An annual safety program audit and walk‐through by REM; and

Recommendation for ISP certification renewal from REM

More information about the ISP program can be found on the REM website

(http://www.purdue.edu/rem/home/files/ispinfo.htm).

1.7 Chemical and Laboratory Safety Committee

Purdue University has established the Chemical and Laboratory Safety Committee (CLSC) with

the responsibility to promote safe and proper chemical management at all Purdue University

Campuses and related facilities. Chemical management includes, but is not limited to, the

procurement and the safe handling, use, storage, and disposal of chemicals. The CLSC reviews

lab safety programs and makes recommendations to the Provost as appropriate. The CLSC

consists of members appointed from the faculty and staff of the major research, teaching, and

service areas where chemicals are handled or used. Although REM facilitates the content of the

CHP, it is ultimately the responsibility of the CLSC to approve changes and updates to the CHP.

Chapt

Chemica

used clas

Labeling

Commun

the sever

classifica

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elements

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standard

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Chapter 2: Chemical Classification Systems

10


1. Identification of the substance or mixture and of supplier

9. Physical and chemical properties

2. Hazards Identification 10. Stability and reactivity

3. Composition/information on ingredients

11. Toxicological information

4. First aid measures 12. Ecological information

5. Firefighting measures 13. Disposal considerations

6. Accidental release measures 14. Transport considerations

7. Handling and storage 15. Regulatory information

8. Exposure controls/personal protection

16. Other information

Figure 2.1 – GHS Required Sections of a Safety Data Sheet

2.1.2 Chemical Labeling

The GHS standardized label elements, which are not subject to variation and must appear

on the chemical label, contain the following elements:

Symbols (hazard pictograms) are used to convey health, physical and environmental

hazard information, assigned to a GHS hazard class and category;

Signal Words such as “Danger" (for more severe hazards) or "Warning" (for less

severe hazards), are used to emphasize hazards and indicate the relative level of

severity of the hazard assigned to a GHS hazard class and category;

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standard phrases assigned to a hazard class and category that describe the nature of

the hazard; and

Precautionary statements are recommended measures that should be taken to

minimize or prevent adverse effects resulting from exposure to the hazardous

chemical.

GHS also standardizes the hazard pictograms that are to be used on all hazard labels and

SDSs. There are 9 pictograms that represent several defined hazards, and include the

harmonized hazard symbols which are intended to convey specific information about each

hazard. Figure 2.2 illustrates these GHS hazard pictograms.

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14

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PA Hazard Ra

Hazard Cla

hazardous m

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15

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16

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essure and a

tandard con

ce of an ignit

mbustible, o

ained in a re

d liquid.

ntains the biv

peroxide, w

nic radicals.

ot necessarily

bute to the c

ously Comb

external ign

Chapter 2: Classe

nline.

hazard class

and handled

ed in the lab

ition to a sp

erencing the

mical as any e

hich is a phys

y classes of c

e that it is fla

, pyrophoric

ed below. R

chemical rea

t such a spe

nditions can

tion source a

r may cause

eceptacle at

valent ‐0‐0‐

here one or

y combustib

combustion

bustible): A l

nition source

es of Hazardous Ch

provides

d. Each chem

boratory, mu

ecific chemi

e SDS for tha

element,

sical or healt

chemicals ar

ammable,

, self‐heatin

efer to Appe

action of

eed as to cau

generate

and have a f

e or contribu

a pressure n

structure an

both of the

ble, may

of other

iquid or soli

e can ignite a

hemicals

mical

ust

cal

at

th

re

g,

endix

use

flash

te to

not

nd

d

after

Chapter 2: Classes of Hazardous Chemicals

17


Self‐Heating Substance: A liquid or solid, other than a pyrophoric substance, which,

by reaction with air and without energy supply, is liable to self‐heat.

Self‐Reactive Substance: A liquid or solid that is liable to undergo strong exothermic

thermal decomposition even without participation of oxygen (air).

Water‐Reactive Substance: A liquid or solid that reacts violently with water to

produce a flammable or toxic gas, or other hazardous conditions.

3.2 Health Hazards

A chemical is a health hazard if there is statistically significant evidence based on at least one

study conducted in accordance with established scientific principles that acute or chronic

health effects may occur in exposed employees. Each health hazard is briefly defined below.

Refer to Appendix B (section B.2) for detailed information on each health hazard.

Carcinogens: Substances that cause cancer. Generally they are chronically toxic

substances; that is, they cause damage after repeated or long‐duration exposure, and

their effects may only become evident after a long latency period. Carcinogens are

separated into two classes: select carcinogens and regulated carcinogens.

Corrosives: Substances that cause destruction of living tissue by chemical corrosion at

the site of contact and can be either acidic or caustic (basic).

Hazardous Substances with Toxic Effects on Specific Organs: Substances that pose

adverse health effects to specific organs such as the liver, kidneys, lungs, etc.

High Acute Toxicity Substances: Substances that may be fatal or cause damage to target

organs as the result of a single exposure or exposures of short duration. Acute toxins are

quantified by a substance’s lethal dose‐50 (LD50) or lethal concentration‐50 (LC50),

which is the lethal dose of a compound to 50% of a laboratory tested animal population

(e.g., rats, rabbits) over a specified time period.

Irritant: Substances that cause reversible inflammatory effects on living tissue by

chemical action at the site of contact.

Reproductive Toxins: Substances that may affect the reproductive capabilities, including

chromosomal damage (mutations) and effects on fetuses (teratogens).

Sensitizer (also called allergen): A substance that causes exposed individuals to develop

an allergic reaction in normal tissue after repeated exposure to the substance.

Chapter 2: Classes of Hazardous Chemicals

18


3.3 Biological Hazards

The Purdue University Institutional Biosafety Committee (IBC) is the campus‐based committee

that has the responsibility for reviewing and approving all proposals, activities, and experiments

involving an organism or product of an organism that presents a risk to humans, plants,

animals, or the environment. The PI must submit to the IBC an application to use rDNA,

synthetic nucleic acids, potential pathogens, human tissue, fluids, and/or cell lines in their

research. The IBC review is conducted in accordance with the guidance and requirements of

National Institutes of Health, the Centers for Disease Control, and Purdue University policies,

and the Biosafety Manual. All PIs have an obligation to be closely familiar with EHS guidelines

applicable to their work and to adhere to them. More detail regarding the IBC process can be

found on the Purdue Office of the Vice President for Research webpage:

(http://www.purdue.edu/research/vpr/rschadmin/rschoversight/rdna/forms.php).

3.4 Radioactive Material Hazards

The Purdue University Radiation Safety Committee (RSC) is the campus‐based committee that

has the responsibility for reviewing and approving all proposals, activities, and experiments

involving radioactive material and radiation producing devices. The PI must submit to the RSC

through REM, an application to use radioactive material or radiation‐producing devices. Use of

radioactive materials at Purdue University is authorized under a license issued by the US

Nuclear Regulatory Commission or a registration with the Indiana State Department of Health

and all work must comply with applicable regulations. The policies and procedures for handling

radioactive materials are contained in the Purdue University Radiation Safety Manual.

(http://www.purdue.edu/rem/home/booklets/radman.pdf)

3.5 Laser Hazards

The Purdue University Laser Safety Committee (LSC) is the campus‐based committee that has

the responsibility for reviewing and approving all proposals, activities, and experiments

involving laser radiation devices. PIs must submit to the LSC through REM, an application to use

Class 3B and Class 4 lasers or laser devices. The use of lasers is subject to OSHA regulations and

utilizes current ANSI standards to develop guidance. The policies and procedures for handling

lasers are contained in the Purdue University Laser Safety Guidelines.

(http://www.purdue.edu/rem/home/booklets/laserguide.pdf)

Chapt

Laborato

Elements

exposure

strategie

from the

eliminati

controls,

responsib

correctly

4.1 R

There are

inhalatio

the labor

people th

measure

4.2 E

Exposure

engineer

chemical

S

fo

Is

w

U

4.2.1

A che

expo

requi

State

Later 4:

ory safety co

s of these th

e to hazardo

es on the pre

workplace o

on or substi

and PPE mu

bility is to fo

y and effectiv

Routes of E

e four prima

n, absorptio

ratory are by

hrough more

s are in plac

ngineering

e to hazardo

ring controls

ls include:

ubstitution w

or glass bott

solation of th

water‐sensiti

Use of forced

Chemical

emical fume

sure to haza

ired to be us

ements foun

The officia

aborato

ntrols includ

hree categor

us chemical

emise that th

or substitute

tution are n

ust be used t

ollow admini

vely.

Exposure

ary routes of

on, ingestion

y inhalation

e than one o

ce for each o

g Controls

us materials

s. Engineerin

with less haz

les);

he operator

ve chemical

d ventilation

Fume Hood

hood is a ty

ardous or tox

sed inside of

d in Section


ry Safet

de engineeri

ies should b

s. The hiera

he best way

e a less haza

ot feasible o

to provide th

istrative con

f exposure in

n, and injecti

and/or skin

of these expo

of these expo

and Safet

s must be co

ng controls to

zardous equ

or the proce

s); and

systems (e.g

ds

ype of local v

xic fumes, va

f a chemical

2 on a SDS s

19

ocument will only

y Contro

ng controls,

e used in a l

archy of cont

to control a

ardous techn

options, adm

he necessary

trols, use en

n which haza

on. Of these

absorption.

osure mode

osure routes

ty Equipme

ontrolled to t

o reduce or

ipment, che

ess (e.g., use

g., chemical

ventilation in

apors, or du

fume hood,

such as “do

y be maintained on

ols

, administrat

layered appr

trols prioriti

hazard is to

nique, proce

ministrative c

y protection

ngineering c

ardous subst

e, the most l

Many haza

s, so it is crit

s.

ent

the greatest

eliminate ex

emicals, or p

e of a glove b

fume hood,

nstallation th

sts. To dete

, first check t

not breathe

Chapter 4: La

nline.

tive controls

roach to min

zes hazard m

o systematic

ess, or mater

controls, eng

n. The labora

ontrols, and

tances can e

likely routes

rdous chem

tical that pro

t extent feas

xposures to

processes (e.

box when ha

, biological s

hat is design

rmine if a ch

the SDS for t

e dust, fumes

aboratory Safety C

s, and PPE.

nimize empl

mitigation

ally eliminat

rial. If

gineering

atory employ

d wear PPE

enter the bo

s of exposure

icals may aff

otective

sible by use o

hazardous

g., safety ca

andling air‐ o

safety cabine

ned to limit

hemical is

that chemic

s, or vapors”

Controls

oyee

te it

yee’s

dy:

e in

fect

of

ans

or

et).

al.

” or

“toxic

chem

solut

used,

c by inhalati

mical fume ho

ions) whene

, the followi

Chemical

optimum

sash shou

chemicals

height sho

any splash

optimum

intended

to add or

Figure

Chemical

device to

interpret

before us

of chemic

understan

The officia

on” indicate

ood for all w

ever possible

ng guideline

fume hoods

hood perfo

uld be positio

s that could

ould be set a

hes, explosio

air flow dyn

to be used w

remove equ

e 4.1 – Chem

fume hoods

indicate ade

this gauge a

ing hazardo

cal fume hoo

nds the spec


e the need fo

work involvin

e. If a chemic

es must be fo

s must be m

rmance as il

oned at this

generate to

at a level wh

ons, or othe

namics are a

with the sash

uipment from

mical Fume H

s must be eq

equacy of flo

and check th

us chemical

ods on camp

cific function

20

ocument will only

or ventilatio

ng the handl

cal fume hoo

ollowed at a

arked to ind

lustrated in

height whe

xic aerosols,

here the ope

r violent rea

chieved. Mo

h fully open

m the chemi

Hood Sash A

quipped with

ow. All lab e

at the chem

s in the fum

pus, so it is im

ns of each ch

y be maintained on

n. As a best

ing of open

od is require

all times:

dicate the pr

Figure 4.1. T

never worki

, gases, or va

erator is shie

actions whic

ost chemical

. The sash sh

ical fume ho

Approved W

h a continuo

mployees m

mical fume ho

e hood. The

mportant th

hemical fum

Chapter 4: La

nline.

practice, alw

chemicals (e

ed or recom

roper sash po

The chemica

ng with haza

apors. In gen

elded to som

h could occu

l fume hood

hould only b

ood.

Working Heig

ous reading m

must know ho

ood is opera

ere are many

at the lab em

e hood used

aboratory Safety C

ways use a

e.g., prepari

mended to b

osition for

al fume hood

ardous

neral, the sa

me degree fro

ur and wher

s are not

be fully open

ght

monitoring

ow to read a

ating proper

y different ty

mployee

d.

Controls

ng

be

d

ash

om

e

ned

and

ly

ypes

Only appa

be placed

experime

fume hoo

Chemical

or materi

dedicated

storage ch

All chemic

(e.g., bea

to label a

temporar

chemical

practical,

Figure 4.2

Do not all

Never put

All chemic

face veloc

velocity re

Contact R

The officia

aratus and c

d in the chem

nts should b

od.

fume hoods

al storage. C

d to chemica

hemical fum

cal containe

kers, flasks,

secondary c

ry label can b

fume hoods

the hood sa

– Alternativ

low the vent

t your head

cal fume hoo

city, which s

eadings on a

REM with qu


hemicals ess

mical fume h

be removed

s used for ex

Chemical fum

al storage. N

me hoods.

ers used in ch

reaction ves

container th

be used as s

s must be lab

ash or wall m

ve Labeling o

ts or air flow

inside of an

ods should b

hould be be

a routine bas

estions rega

21

ocument will only

sential to th

hood. Extran

and stored i

xperimental

me hoods us

o experimen

hemical fum

ssels, vials, e

at is in proce

hown in Sec

beled as wel

may be labele

of Chemical

w baffles to b

operating c

be routinely

etween 70 –

sis and reco

arding chem

y be maintained on

e specific pr

eous materi

in a safe loca

work should

sed for chem

ntal work sh

me hoods, inc

etc.) must be

ess (e.g., rea

ction 5.7 of t

ll. If labeling

ed as illustra

l Fume Hood

be blocked.

hemical fum

checked for

125 feet pe

rds this info

ical fume ho

Chapter 4: La

nline.

rocedure or

ials from pre

ation outsid

d not be use

mical storage

hould be con

cluding seco

e labeled. If

action vesse

the CHP. Rea

g the vessel i

ated in Figur

d Reaction V

me hood.

r airflow by m

r minute. RE

rmation on

oods (765) 4

aboratory Safety C

process sho

evious

e the chemi

ed for chemi

e should be

nducted in

ondary conta

is not practi

l, flask), a

action vesse

tself is not

re 4.2.

Vessels

measuring t

EM conducts

the hood lab

9‐46371.

Controls

ould

cal

cal

ainers

ical

ls in

he

s face

bel.

4.2.2

A glov

handle

sensit

should

Glove Box

ve box, as illu

e material in

ive items ins

d be followe

All person

work in a

features a

instructio

pressure

controls (

Prior to u

be docum

change). G

to the glo

Plugging p

plugged p

Chemical

glove box

The glove

after glov

The officia

xes

ustrated in F

n a defined a

side or the u

d by all pers

nnel must re

glove box o

and limitatio

on on elemen

differential b

e.g., control

se, a visual g

mented (date

Gloves shou

ove box man

ports that ar

port should h

resistant glo

x gloves to p

e box pressu

ves are chang


Figure 4.3, is

atmosphere

user on the o

sonnel using

Figure 4

eceive docum

ccurs. All tra

ons of a glov

nts such as t

between the

lling oxygen

glove inspec

e, manufactu

ld not be us

ufacturer’s r

re never or i

have a stub g

oves (e.g., di

rotect from

re must be c

ged. The pre

22

ocument will only

a sealed co

(typically in

outside, or b

g a glove box

4.3 – Glove

mented train

ained person

e box before

the ventilatio

e glove box a

concentrati

ction must be

urer, model

ed until they

recommend

nfrequently

glove and a

isposable nit

contaminat

checked eve

essure check

y be maintained on

ntainer that

ert). Glove b

both. The fol

x:

Box

ning from th

nnel must un

e use. The tr

on and vacu

and outside

ions and mo

e performed

of glove, an

y fail; they s

dations or wh

y used is reco

glove port c

trile gloves)

ion.

ery day, befo

k must be do

Chapter 4: La

nline.

t is designed

boxes can be

lowing reco

e PI or deleg

nderstand th

raining must

um controls

atmosphere

oisture), etc.

d. Changing

d person pe

should be ch

henever nec

ommended.

cap installed

should be u

ore use and i

ocumented.

aboratory Safety C

to allow on

e used to pro

mmendation

gate before

he design

t include det

s that mainta

e, atmosphe

of a glove m

erforming

hanged accor

cessary.

A properly

.

used under t

immediately

Controls

e to

otect

ns

any

tailed

ain a

eric

must

rding

he

y

4.2.3

A lam

preve

Air is

There

mate

towa

chem

4.2.4

A bio

labor

conta

serve

Keep shar

Do not wo

Follow all

associate

All equipm

must be l

glove box

Laminar F

minar flow cl

ent contami

drawn thro

efore it is cri

erials ever be

rds the user

mical fume ho

4 Biologica

ological (or b

ratory works

aminated wi

e as a means

The officia

rps in an app

ork in the glo

safe work p

d with work

ment and ch

abeled. Do n

x.

Flow Clean B

ean bench, a

nation of se

ugh a filter a

itical that ab

e used in a la

r. Application

oods.

Figu

l Safety Cab

iosafety) saf

space for saf

th) infectiou

s to protect t


proved conta

ove box unle

practices for

ing in the glo

hemicals in th

not allow ite

Benches

as shown in

miconducto

and blown in

bsolutely no

aminar flow

ns that invol

ure 4.4 – Lam

binets

fety cabinet,

fely working

us materials.

the laborato

23

ocument will only

ainer while i

ess the light

using and h

ove box.

he glove box

ems, particul

Figure 4.4, i

r wafers, sam

n a very smo

hazardous c

clean bench

lve the use o

minar Flow C

, as shown in

with materi

. The primar

ory worker a

y be maintained on

in the glove

ing is workin

handling com

x must be or

larly chemic

is an enclose

mples, or an

ooth, lamina

chemicals, in

h, as the vap

of chemicals

Clean Bench

n Figure 4.5,

ials contami

ry purpose o

nd the surro

Chapter 4: La

nline.

box.

ng.

mpressed gas

rganized and

als to accum

ed bench de

ny particle se

ar flow towa

nfectious and

pors are blow

s should be c

h

, is an enclos

inated with

of a biosafety

ounding env

aboratory Safety C

s that may b

d all chemica

mulate in the

signed to

ensitive devi

rds the user

d/or radioac

wn directly

conducted in

sed, ventilat

(or potentia

y cabinet is t

vironment fro

Controls

be

als

e

ice.

r.

ctive

n

ted

lly

to

om

patho

partic

so th

chem

4.2.5

All la

and e

the c

with

awar

statio

some

and/o

begin

deco

All ey

prope

grow

show

of ea

ogens. All ex

cles. Biologic

e use of che

micals should

Safety Sh

boratories u

eye wash sta

enterline of

a highly visib

e of the loca

ons. If lab pe

eone else in

or eye wash

n treatment.

ntamination

yewash stati

er working o

wth from red

wer and eyew

ch station. A

The officia

xhaust air is

cal safety ca

emicals shou

d be conduct

Fig

owers and E

using hazard

ations. Safety

the spray pa

ble sign and

ation and kn

ersonnel are

the lab that

unit for 15

. If an uninju

n of the affec

ons must be

order. This w

ucing perfor

wash station

Any deficien


filtered as it

binets are n

ld be kept to

ted in chemi

ure 4.5 – Bio

Eyewash Sta

ous chemica

y showers m

attern in all

maintain an

ow how to p

exposed to

is not expos

minutes or u

ured individu

cted individu

e flushed by

will keep the

rmance. REM

s. This inspe

cies are repa

24

ocument will only

t exits the bi

not designed

o a minimum

ical fume ho

ological Safe

ations

als must hav

must have a m

directions a

n unobstruct

properly use

a hazardous

sed should d

until emerge

ual is presen

ual.

laboratory p

system free

M performs a

ection evalua

aired either

y be maintained on

osafety cabi

d to be used

m. Applicatio

oods.

ety Cabinet

ve immediate

minimum cle

t all times. I

ted path to i

e the safety s

s chemical, t

dial 911) and

ency respons

t, this perso

personnel on

e of sedimen

annual inspe

ates the bas

by REM staf

Chapter 4: La

nline.

inet, removi

with chemic

ons that invo

e access to s

earance of 2

dentify the s

it. All lab per

shower and

they should

d use the saf

se have pers

on should ass

n a weekly b

nt and preve

ections of al

ic mechanic

ff or by Purd

aboratory Safety C

ng harmful

cal applicatio

olve the use

safety showe

24 inches fro

safety statio

rsonnel mus

eyewash

dial 911 (or

fety shower

sonnel arrive

sist with the

basis to ensu

nt bacterial

l campus saf

cal functiona

due Physical

Controls

ons

of

ers

om

on

st be

e and

e

ure

fety

ality

Chapter 4: Laboratory Safety Controls

25


Facilities maintenance staff. If the safety shower or eye wash unit becomes inoperable,

notify your building deputy immediately.

4.2.6 Fire Extinguishers

All fire extinguishers should be mounted on a wall in an area free of clutter. Each fire

extinguisher on campus is inspected on an annual basis by the Purdue Fire Department. All

laboratory personnel should be familiar with the location, use, and classification of the

extinguishers in their laboratory. Ensure that the fire extinguisher being used is appropriate

for the type of material on fire before attempting to extinguish any fire. Table 4.1 illustrates

the fire classification system, which should be used to determine the most suitable fire

extinguisher for a particular area. Laboratory personnel are not required to extinguish fires

that occur in their work areas and should not attempt to do so unless:

It is a small, contained fire that can be quickly and safely extinguished (e.g., small

trash can sized fire);

Appropriate training has been received and the individual feels the fire can be safely

extinguished; and

It is necessary to extinguish a fire in order to exit an area (e.g., fire is blocking an

exit).

If a fire occurs in the laboratory and is extinguished by lab personnel, the Purdue University

Fire Department must still be contacted immediately by dialing 911.

Table 4.1 – Fire Classifications System

Classification Fire Type Class A Ordinary fire (wood and paper)

Class B Flammable liquids and gases

Class C Electric fire

Class D Combustible metal fire

Class K Kitchen fire

4.2.7 Fire Doors

Many laboratories may contain fire doors as part of the building design. These doors are an

important element of the fire containment system and should remain closed unless they are

on a magnetic self‐closure or other automated self‐closing system. Never disable an

automatic door closure device (e.g., placing a block under the door). If you are unsure of

whether a door is fire rated or not, contact REM at (765) 49‐46371 and a staff member will

come to the area to evaluate the specific door in question.

4.3 A

Administ

hazards a

following

E

d

m

C

sa

w

d

R

U

La

S

G

re

b

P

4.3.1

SOPs

proce

mitig

know

Supe

The O

perfo

chem

Administra

trative contr

associated w

g:

nsuring that

ocumented

materials

areful plann

afety in mind

written SOPs

etail in Chap

estricting ac

Using safety s

abeling all ch

ubstitution o

Good housek

egular decon

ench‐tops a

rohibiting ea

Standard

are written

edure and in

gated. SOPs m

wledgeable a

rvisor is ultim

OSHA Lab Sta

ormed in the

micals:

Explosive

Water‐rea

Particular

and acute

Compress

The officia

tive Contr

rols are proc

with the use

t employees

training for

ning of exper

d. Planning i

and hazard

pter 6) for sa

ccess to area

signs or plac

hemicals

of toxic mate

keeping and

ntamination

nd fume hoo

ating and dr

Operating P

instructions

nclude inform

must be pre

nd involved

mately respo

andard requ

e lab. High ha

s

active, pyrop

rly hazardou

ely toxic sub

sed gases


rols

edural meas

of hazardou

are provide

safe work w

riments and

includes the

assessment

afe performa

as where haz

cards to iden

erials with le

good person

of areas tha

ods

inking wher

Procedures

s that detail

mation abou

pared by lab

with the ex

onsible for a

uired SOPs to

azard tasks i

phoric, self‐h

s substance

stances

26

ocument will only

sures which

us materials.

ed adequate

with hazardo

procedures

developme

ts (discussed

ance of the w

zardous mat

ntify hazardo

ess toxic ma

nal hygiene s

at are possib

e chemicals

the steps th

ut potential h

boratory per

xperimental

approving SO

o be develop

include any w

heating, or s

s, which incl

y be maintained on

can be take

Administrat

ous

with

ent of

d in

work

terials are us

ous areas (de

terials, whe

such as rout

bly chemical

are used or

hat will be pe

hazards and

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27

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Chapter 4: Laboratory Safety Controls

28


The Abbreviations, Acronyms, and Chemical Formulas list is required for all labs that

use abbreviations, acronyms, and/or chemical formulas as a means to label chemical

containers, including secondary containers such as beakers, flasks, and vials. This list,

which can be found on the REM webpage, is not all inclusive and any abbreviations

not listed must be added by laboratory personnel.

(http://www.purdue.edu/rem/home/files/guide.htm)

There are several other lab postings that may also be required that are not discussed in the

CHP, particularly if radioisotopes and/or biological agents are used in the lab. This

information should be obtained by reviewing the Radiation Safety Manual and/or Biological

Safety Manual. Additional information regarding lab postings and labels can be found on

the REM webpage. (http://www.purdue.edu/rem/home/files/contlabl.htm)

4.4 Personal Protective Equipment (PPE)

Personal protective equipment (PPE) should be used to supplement engineering controls.

However, PPE should never be used as a substitute for engineering controls when engineering

controls are required. PPE must be worn at all time in the laboratory when handling hazardous

chemicals. Proper PPE selection can be determined in the following ways:

Ask the Laboratory Supervisor about proper PPE selection.

Review the SOP and associated hazard assessment for the task to be performed.

Review Section 8, “Exposure Controls/Personal Protection” of the SDS for the

chemical(s) being used. This will provide basic information on the PPE recommended for

use with the particular chemical. The SDS addresses "worst case" conditions; therefore,

all the equipment described may not always be necessary for a specific job. In addition,

the SDS may not provide sufficient information concerning a specific respirator or type

of glove appropriate for the chemical.

Additional PPE requirements are detailed in the Laboratory PPE Policy in Chapter 6 and Chapter

9 of the CHP.

Chapt

An effect

Requirem

storage,

known by

Purdue.

5.1 L

All labora

chemical

or solid)

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5.1.1

Many

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tive laborato

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segregation

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atory emplo

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of the chem

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Laborator

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What kind

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to protect

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The officia

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process gene

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the proper

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work?


ry Mana

ment plan is

lab houseke

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nel. This chap

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have a good

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type of faci

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uestions

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many of the f

mable, explos

and if so, ho

ccidental ing

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g., chemical‐

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olent chemi

procedures

training to h

ties appropri

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29

ocument will only

agement

essential to

eeping, chem

cals, and equ

pter details h

understandi

Basic factors

lities and eq

hazardous m

safety. Askin

factors that

sive, corrosiv

ow can I be e

gestion, acci

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‐resistant glo

toxic compou

ical reaction

for disposal

handle the c

iate for the t

atible chemi

ur and what

ent? What i

y be maintained on

t Plan

o operating a

mical invento

uipment safe

how laborat

ing of the ha

s such as the

quipment inv

materials occ

ng and answe

should be c

ve, or reactiv

exposed to t

idental punc

t myself?

oves, respira

unds, or cou

?

of the chem

hemicals an

type of mate

icals?

steps can I t

s the worst

Chapter 5: Labo

nline.

a safe lab en

ories, proper

ety must be

tories should

azards assoc

e physical st

volved with

curs.

ering the fol

considered w

ve?

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cture)?

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micals?

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take to mini

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the procedu

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be using?

mize the

at could resu

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and

ed at

he

uid,

ure

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?

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Chapter 5: Laboratory Management Plan

30


5.1.2 General Laboratory Safety Rules

It is extremely important that all laboratory safety rules are known and followed by lab

personnel. Not only is it important that the rules are understood and followed, it is also

important that the Laboratory Supervisor enforce all lab safety rules. A culture of safety

must be adopted by all employees before a lab safety program can be successful. The

following general laboratory safety rules should be followed at all times:

Prior to beginning work in the lab, be prepared for hazardous materials emergencies

and know what actions to take in the event of an emergency. Plan for the worst‐case

scenario. Be sure that necessary supplies and equipment are available for handling

small spills of hazardous chemicals. Know the location of safety equipment such as

the nearest safety shower and eyewash station, fire extinguisher, spill kit, and fire

alarm pull station.

Do not work alone in the laboratory if you are working with high hazard materials

(e.g., acutely toxics, reactives, or processes that involve handling a large volume of

flammable materials, > 1 liter).

If working with a high‐hazard chemical, ensure that others around you know what

you are working with and understand the potential hazards.

Limit access to areas where chemicals are used or stored by posting signs and/or

locking doors when areas are unattended.

Purchase the minimum amount of hazardous materials necessary to efficiently

operate the laboratory.

Ensure that adequate storage facilities (e.g., chemical storage rooms, flammable

safety cabinets) and containers are provided for hazardous materials. Ensure that

hazardous materials are properly segregated by chemical compatibility.

Ensure that ventilation is adequate for the chemicals being used. Understand how

chemical fume hoods function and be able to determine if the hood is not

functioning properly.

Use good personal hygiene practices. Keep your hands and face clean; wash

thoroughly with soap and water after handling any chemical.

Smoking, drinking, eating, and the application of cosmetics are forbidden in areas

where hazardous chemicals are in use. Confine long hair and loose clothing.

Never smell or taste a hazardous chemical. Never use mouth suction to fill a pipette.

When using equipment that creates potential hazards (e.g., centrifuge), ensure that

the equipment is being used following the manufacturer’s guidelines and

instructions. If equipment requires routine maintenance (e.g., HEPA filters need to

be changed), ensure the maintenance is performed by a qualified individual.


31


Use required PPE as instructed by the PPE Policy detailed in Chapter 6.

5.2 Housekeeping

Housekeeping is an important element to a laboratory safety program. A clean, well‐maintained

lab improves safety by preventing accidents and can enhance the overall efficiency of the work

being performed. The following laboratory housekeeping guidelines should be followed:

All doorways and hallways must be free of obstructions to allow clear visibility and exit.

The laboratory should be uncluttered without excessive storage of materials that could

cause or support a fire (e.g., paper, cardboard, flammable liquids, etc.).

Fire protection sprinklers must be unobstructed; a minimum of 18 inches of clearance is

required below the sprinkler head. If the laboratory does not have fire protection

sprinklers, there must be a minimum of 24 inches of clearance below the ceiling.

Do not store items that block fire extinguishers or eyewash and safety shower stations.

Do not store items in front of electrical boxes/panels in the lab.

A routine cleaning schedule should be established. All work surfaces should be kept as

clean as possible. All potentially chemically contaminated work area surfaces (e.g.,

chemical fume hood deck, countertops) should be cleaned routinely (e.g. daily, weekly).

For operations where spills and contamination are likely (e.g., agarose gel

electrophoresis/ethidium bromide applications), cover work spaces with a bench paper

or liner. The soiled bench paper should be changed on a routine basis or as needed.

All chemical spills must be cleaned up immediately. Refer to Chapter 8 of the CHP for

detailed chemical spill cleanup procedures.

Do not allow materials to accumulate in laboratory hoods and remove used tissues, foil,

gloves, or other unnecessary objects immediately after use. The safety of the workspace

and the hood ventilation may be compromised when excessive chemicals and

equipment are kept in hoods.

Ensure that all waste (e.g., trash, chemically contaminated waste, etc.) is placed in the

appropriate containers. Do not overfill waste containers.

All equipment should be cleaned and returned to storage after each use.

Equipment should be stored in a safe and orderly manner that prevents it from falling.

Chemical containers must be clean, properly labeled, and returned to storage upon

completion or usage. Avoid storing liquids above eye level.

Do not store heavy or frequently used items on top shelves. Locate items used daily

close to the work area.

5.3 C

It is a pru

inventory

chemical

Dependin

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of Home

5.4 S

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Every che

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ls. REM reco

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afety Data

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ortant that a

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tions, chem

ete and up‐to

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Facility Anti‐

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an storing h

mental main

all lab perso

manufacturer

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hazard.com/

earch (http:/

equiremen

nt in the labo

erly labeled.

bottles, beak

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e, or structu

nd (e.g., MeO

Most chemica

s all of the n

32

ocument will only

tain a chemi

wn chemicals

tories take a

sed and stor

room, work

‐Terrorism S

on that is imp

el and SDS a

re using a ch

boratory Sup

ccess to SDS

ard copies o

office), or c

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oratory, whe

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oid using

ure unless th

OH = Metha

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(765) 49‐463

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33


information, so care should be taken to not damage or remove these labels. It is recommended

that each bottle also be dated when received and when opened to assist in determining which

chemicals are expired and require proper disposal. Detailed information and strategies for the

labeling of research samples is discussed in Section 5.9 of the CHP. These same strategies can

be used when labeling secondary chemical containers as well.

5.6 Chemical Segregation

All chemicals must be stored according to chemical compatibility. Once segregated by chemical

compatibility, they can then be stored alphabetically. Information regarding chemical

compatibility can be found in the SDS, primarily in Section 7, “Handling and Storage” and

Section 10, “Stability and Reactivity”. If unsure of proper segregation procedures, contact the

Laboratory Supervisor for assistance. Chemical segregation can be achieved by either isolation

(e.g., organic solvents stored in a flammable cabinet), physical distance (e.g., acids and bases

are stored on opposite sides of a chemical storage room), or secondary containment (e.g.,

placing oxidizing acids such as nitric acid into a secondary containment to segregate from

organic acids such as formic acid as shown in Figure 5.7). In the most general terms, proper

segregation can be achieved by:

Storing acids away from bases and toxics;

Storing oxidizers away from organic chemicals; and

Storing reactive and acutely toxic materials away from all other chemicals.

Table 5.1 illustrates a more detailed chemical compatibility logic that can be used for chemical

storage. Hazard classes marked by an X need to be segregated from each other (e.g., Acid,

inorganic must be segregated from Base, inorganic). Contact REM at (765) 49‐40121 with

questions regarding chemical segregation.


34


Table 5.1 – Chemical Compatibility Chart

Acid,

inorganic Acid, organic

Acid, oxidizer

Base, inorganic

Base, organic

Oxidizer Toxic,

inorganic Toxic, organic

Reactive Organic solvent

Acid, inorganic X X X X X

Acid, organic X X X X X X X

Acid, oxidizer X X X X X X X

Base, inorganic X X X X

Base, organic X X X X X

Oxidizer X X X X X Toxic,

inorganic X X X X

Toxic, organic

X X X X X

Reactive X X X X X X X X X Organic solvent

X X X

5.7 Chemical Storage Requirements

Proper storage of chemicals is an essential component to a laboratory safety program.

Improper chemical storage practices can cause undesired chemical reactions, which may form

hazardous products that can lead to employee exposure or possibly fires and property damage.

All lab employees should carefully read each chemical’s SDS and container label before deciding

how to store a chemical, as these will often indicate any special storage requirements that may

be necessary. The following subsections describe chemical storage requirements in more detail.

5.7.1 General Chemical Storage

The following general chemical storage guidelines must be followed in all laboratories:

Each chemical in the laboratory must be stored in a specific location and returned

there after each use. Acceptable chemical storage locations may include flammable

cabinets, corrosive cabinets, laboratory shelves, or appropriate laboratory

refrigerators or freezers.

Chemical containers must be in good condition and appropriate for the chemical

that they contain and be free from exterior contamination.

Fume hoods should not be used as permanent chemical storage areas, unless

designated as such. Not only does this create potentially unsafe conditions by having

extraneous chemicals stored near chemical reactions and processes, excess chemical


35


bottles in the hood may also seriously impair the ventilating capacity of the hood.

Only chemicals being used in the process or experiment being conducted in the

hood are allowed to be stored in the hood and should be removed when the process

or experiment is complete.

Chemicals should not be permanently stored on bench tops. Avoid storing any

chemical containers on the floor. Under no circumstance should chemical

containers, or anything else, be stored in aisle ways, corridors, or in front of doors.

Hazardous liquids should not be stored on shelves above eye‐level unless there is a

SOP detailing safe handling procedures.

Chemicals should be stored at an appropriate temperature and humidity level and

never be stored in direct sunlight.

Periodic cleanouts of expired or unneeded chemicals should be conducted to

minimize the volume of hazardous chemicals stored in the laboratory.

Always follow the chemical manufacturer’s storage instructions, if provided.

5.7.2 Flammable Liquids Storage

Flammable liquids include any liquid with a flash point no greater than 93 °C (200 °F). The

following guidelines for storing flammable liquids must be followed in all laboratories:

Flammable and combustible liquids should be stored in flammable storage cabinets,

as shown in Figure 5.1, whenever possible. No more than 10 gallons of flammable

liquid is permitted to be stored outside of a flammable storage cabinet unless it is

stored in a flammable safety can equipped with a spring‐loaded lid and an internal

screen as shown in Figure 5.2.

Domestic refrigerators or freezers must never be used to store flammable liquids.

Flammable liquids can only be stored in refrigerators or freezers that are designed

for flammable materials (most refrigerators are not intended for flammable

storage).

Flammable liquids must be stored in well‐ventilated areas free from ignition sources.

Some organic solvents (e.g., diethyl ether) have a shelf‐life and can form organic

peroxides over time while in storage. These “peroxide formers” must be dated when

received from the chemical manufacturer and disposed of once expired. If any time‐

sensitive chemicals are found to be past the manufacturer’s expiration date, they

must be submitted to REM for hazardous waste disposal immediately. See Appendix

C for a list of commonly found organic solvents that potentially form organic

peroxides.

Fig

5.7.3

Comp

less t

comp

gure 5.1 – Fla

Compress

pressed gase

than 280 kPA

pressed gase

Compress

location, a

All cylinde

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the safety

they are c

restrainin

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secured b

should be

The officia

ammable St

sed Gases St

es are define

A at 20 °C or

es must be fo

sed gas cylin

and in an up

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that are not

y cap. Multip

capped (not

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that are in u

by a chain or

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orage Cabin

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ed as gases t

r as a refrige

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nders (cylind

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t in use (mea

ple cylinders

in use). Only

ang chained)

use, meanin

r strap as sho

from impact

36

ocument will only

net

that are cont

rated liquid.

ll laboratori

ers) must be

on at all time

s if full and s

aning that th

s may be sec

y capped cy

) as shown in

g there is a r

own in Figur

t or damage.

y be maintained on

Figure 5.2 –

tained in a r

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e stored in a

es.

should neve

he cap is on)

cured togeth

linders can b

n Figure 5.3.

regulator att

re 5.4. Cylind

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Chapter 5: Labo

nline.

– Flammable

receptacle at

ing guideline

a secure, we

er be comple

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her (gang‐ch

be secured w

.

tached, mus

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oratory Manageme

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t a pressure

es for storing

ll ventilated

etely emptie

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n

not

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5.7.4

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Figure 5.3 –

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posted to

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– Not In‐Use

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ls include ex

water‐react

flammable

etal immers

ls include co

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unt of reactiv

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ve materials

ould have th

ot be deface

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ment practice

storage locat

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o indicate the


e Cylinders

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tives. Many

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must be cle

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d in any way

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tions for rea

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eir presence

37

ocument will only

yrophorics, s

reactive ma

g., lithium alk

al oil). Other

eratogenicity

materials m

s stored in th

materials mu

early labeled

name, hazar

y.

placed into s

ctive materi

cabinets that

sically safe r

patible chem

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y be maintained on

Figure 5.4 –

self‐heating

terials are a

kyl compoun

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ust be follow

he lab must

ust be prope

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rd labels, and

econdary co

ials include i

t do not con

refrigerators

micals. If poss

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e hazards as

Chapter 5: Labo

nline.

– In‐Use Cyli

and self‐rea

lso toxic and

nds dissolved

azards often

c peroxide fo

wed in all lab

be kept to a

erly disposed

riginal manu

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ontainment a

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sible, store a

or reactives.

shown in Fig

oratory Manageme

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d are dissolv

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n associated

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boratories:

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d.

Toxic Materi

terials are d

the result of

nes for stori

storage locat

mmable sto

y strong acid

e clearly post

oxic materia

agement pra

e, store all a

erials. Signs


re 5.5 – Rea

ials are wate

nd/or water.

d in such a w

are received

(such as the

container is m

e, or inert ga

als Storage

efined as su

f a single exp

ng acutely t

tions for acu

orage cabine

ds), or non‐d

ted.

ls should be

actice.

cutely toxic

should be p

38

ocument will only

ctive Chemi

er and/or air

. Therefore,

way that rigo

in a speciall

e Aldrich Sur

maintained.

s remains in

bstances tha

posure or ex

oxic materia

utely toxic m

ts that do no

omestic refr

e stored in se

materials in

posted to ind

y be maintained on

icals Storage

r reactive an

reactives m

orously exclu

ly designed s

re‐Seal packa

Ensure that

n the contain

at may be fa

xposures of s

als must be f

materials incl

ot contain in

rigerators or

econdary co

n a cabinet d

dicate their p

Chapter 5: Labo

nline.

e

nd can spont

must be hand

udes air and

shipping, sto

aging system

t sufficient p

ner while rea

atal or cause

short duratio

followed in a

ude desicca

ncompatible

r freezers. Th

ntainment a

edicated on

presence an

oratory Manageme

taneously ign

dled under a

moisture.

orage, or

m), ensure th

protective

active mater

e damage to

on. The

all laborator

tors, glove

e chemicals

hese locatio

at all times a

nly for acutel

d unique

ent Plan

nite

n

hat

rials

ries:

ns

as a

ly

5.7.6

Corro

chem

stora

wher

chem

cyani

oxidiz

with

meth

5.7.7

Oxidi

comb

other

The amou

Any expir

waste.

All acutely

label, whi

label shou

Corrosive

osive materi

mical corrosio

ge method f

re acids and

micals where

ide compoun

zing acids (e

each other.

hod) or by se

F

Oxidizers

zing materia

bustible, may

r material. A

The officia

unt of acutel

ed or unnec

y toxic mate

ich should h

uld not be de

e Materials S

als are defin

on at the site

for corrosive

bases are se

a toxic gas w

nds). Organi

e.g., nitric ac

Segregation

econdary con

Figure 5.6 –

and Organi

als are defin

y generally b

An organic pe

Acetic Acid

Formic Acid


ly toxic mate

cessary mate

erials should

ave the chem

efaced in an

Storage

ned as substa

e of contact

e materials i

egregated at

would be ge

c acids (e.g.,

id, perchlori

n can be ach

ntainment a

Segregation

c Peroxide S

ed as substa

by yielding o

eroxide is an

d

39

ocument will only

erial stored i

erials must b

be clearly la

mical name,

ny way.

ances that c

and can be

s inside of a

t all times. A

enerated upo

, acetic acid,

ic acid), as th

ieved either

s shown in F

n Using Seco

Storage

ances which,

oxygen, caus

n organic sub

y be maintained on

in the lab sh

be properly d

abeled with

, hazard labe

ause destru

either acidic

corrosive st

Acids must al

on contact w

, formic acid

hese types o

r by physical

Figure 5.6.

ondary Cont

, while in its

se, or contrib

bstance whic

Nitr

Chapter 5: Labo

nline.

hould be kep

disposed of

the original

els, and picto

ction of livin

c or basic (ca

torage cabin

lso be segreg

with an acid

d) must be st

of acids are i

distance (p

tainment

self are not n

bute to the c

ch contains

ric Acid

oratory Manageme

pt at a minim

as hazardou

manufactur

ograms. The

ng tissue by

austic). The

net or lab ca

gated from

(e.g., reactiv

tored away f

ncompatible

referred

necessarily

combustion

the bivalent

ent Plan

mum.

us

rer’s

e

best

binet

ve

from

e

of

t ‐O‐

O‐ str

of the

storin

5.7.8

A num

and/o

follow

ructure and

e hydrogen a

ng oxidizers

Oxidizers

methyl et

location a

paper, an

If possible

oxidizers.

The amou

minimum

All materi

chemical

Refrigera

mber of gen

or freezers i

wing proced

Domestic

flammabl

stored in

flammabl

flammabl

Lab refrig

beverage

posted w

All chemic

Ensure th

compatib

oxidizer s

chemicals

There mu

must be c

Chemicals

prevent p

The officia

may be con

atoms have

and organic

(e.g., hydro

thyl ketone p

and kept awa

d organic ch

e, store all st

unt of oxidiz

m.

ial must be c

name, hazar

tors and Fre

eral precaut

n the labora

ures must b

refrigerator

e liquids. Fla

refrigerator

e materials

e storage).

gerators or fr

s for consum

ith a sign tha

cals stored i

at the chem

le with each

uch as hydro

s.

ust not be an

completely s

s should be

pressure buil


sidered a de

been replac

c peroxides m

gen peroxid

peroxide, be

ay from com

hemicals (e.g

trong oxidizi

ers and orga

clearly labele

rd labels, an

eezers Chem

tions need to

atory. When

e followed:

rs or freezer

ammable liq

s or freezers

(most refrig

reezers mus

mption. Lab r

at states “No

n a refrigera

micals stored

h other. For e

ogen peroxid

ny open chem

sealed or cap

allowed to w

ldup.

40

ocument will only

erivative of h

ced by organ

must be follo

e, sodium n

enzoyl perox

mbustible ma

g., organic so

ing agents in

anic peroxid

ed; the origi

nd pictogram

mical Storage

o be taken w

working wit

rs must neve

uids are onl

s that are de

erators are

t never be u

refrigerators

o Food or Dr

ator or freez

in a refriger

example, do

de in a refrig

micals in a re

pped and saf

warm to roo

y be maintained on

hydrogen pe

nic radicals. T

owed in all la

itrate) and o

xide) must be

aterials such

olvents and

n a chemical

es stored in

inal manufac

ms should no

e

when storing

th freezers o

er be used to

y allowed to

esigned for

not intende

used to store

s/freezers sh

rink”.

zer must be l

rator or free

o not store a

gerator with

efrigerator o

fely stored.

m temperat

Chapter 5: Labo

nline.

eroxide, whe

The followin

aboratories:

organic pero

e stored in a

h as wood, p

organic acid

cabinet ded

the lab sho

cturer’s labe

ot be defaced

g chemicals i

or refrigerato

o store

o be

d for

e food or

hould be

labeled.

ezer is

n

h organic

or freezer. A

ture before s

oratory Manageme

ere one or bo

ng guidelines

:

oxides (e.g.,

a cool, dry

ressboard,

ds).

dicated only

uld be kept

el with the

d or covered

in refrigerat

ors, the

All containers

sealing to

ent Plan

oth

s for

for

at a

d.

ors

s


41


Shelves in refrigerators or freezers should all have suitable plastic trays for

secondary containment in the refrigerator and freezer compartments. If plastic trays

are not available, liquid chemicals should be placed in secondary containers to

contain spills.

Remember that power outages and technology failures can cause internal

temperatures to rise, which can impact chemical contents. Be aware of unusual

odors, vapors, etc., when opening the refrigerator or freezer.

An inventory should be posted on the refrigerator door.

Chemical refrigerator or freezers should be located away from laboratory exits.

Refrigerators and freezers should be cleaned‐out and manually defrosted as

necessary.

When defrosting a freezer, consideration should be taken regarding potential

chemical contamination of the water. If the water draining from a defrosted

refrigerator may be chemically contaminated, contact REM at (765) 49‐40121 for

further assistance.

5.8 Compressed Gas Cylinder Safety

Compressed gas storage requirements are discussed above in Section 5.7.3. However, there

are additional important safety requirements for use of compressed gases in laboratories

detailed below:

Gas cylinder connections and fittings must be inspected frequently for deterioration.

Never use a leaking, corroded, or damaged cylinder and never refill compressed gas

cylinders.

When stopping a leak between cylinder and regulator, always close the valve before

tightening the union nut.

The regulator must be replaced with a safety cap when the cylinder is not in use.

The safety cap must be in place when a gas cylinder is moved. For large gas cylinders

(>27 inches), an approved gas cylinder cart should be used.

The cylinder must be strapped to the cart and the protective cap must be in place

before moving the cylinder. A cylinder should never be moved or transported without

the protective cap. The proper way to move a large gas cylinder is illustrated in Figure

5.7.

Never dispense from a cylinder if it is on a gas cylinder cart.

A few co

on. It is e

time‐sen

submitte

sensitive

H

H

H

H

H

The com

strictly fo

storage p

(HBr) cyli

moisture

and/or H

walls of t

increases

dangerou

cylinder t

mpressed ga

extremely im

sitive gases

ed to REM fo

compressed

Hydrogen Flu

Hydrogen Bro

Hydrogen Sul

Hydrogen Cya

Hydrogen Ch

pressed gase

ollowed. The

past the exp

inders have

e can slowly

HBr slowly re

the cylinder

s due to the

us projectile

that explode

The officia

as cylinders

mportant tha

are found to

or hazardous

d gases:

uoride, anhy

omide, anhy

lfide, anhydr

anide, anhyd

loride, anhy

es listed abo

ere have bee

iration date

a shelf‐life o

enter the cy

eact with the

weaken due

hydrogen g

es and a toxic

ed in a Purdu


Figure 5.7 –

have a shelf

at these chem

o be past the

s waste dispo

ydrous

ydrous

rous

drous

drous

ove have a sh

en numerous

. For examp

of one to two

ylinder, whic

e internal me

e to the corr

eneration. U

c gas release

ue Universit

42

ocument will only

– Gas Cylind

f‐life and can

micals are id

e manufactu

osal immedi

helf‐life prov

s incidents i

le, hydrogen

o years, dep

ch initiates c

etal walls of

osion, while

Ultimately, th

e. Figure 5.8

y laboratory

y be maintained on

der Cart

n become m

dentified and

urer’s expira

iately. The fo

vided by the

nvolving the

n fluoride (H

pending on t

orrosion. As

the cylinder

e at the same

hese cylinde

8 shows a 30

y in 2011.

Chapter 5: Labo

nline.

more hazardo

d managed p

ation date, th

ollowing is a

e manufactu

ese compoun

HF) and hydro

he vendor. O

s the corrosio

r to produce

e time the in

ers fail and c

‐year old HF

oratory Manageme

ous as time g

properly. If a

hey must be

a list of time‐

rer that mus

nds related t

ogen bromid

Over time,

on continue

e hydrogen. T

nternal press

create extrem

F lecture bot

ent Plan

goes

any

e

‐

st be

to

de

es, HF

The

sure

mely

ttle

5.9 C

A cryoge

most com

cryogenic

human ti

following

U

W

cr

lo

C

o

Ex

in

re

A

te

P

u

Figu

Cryogenic L

nic liquid is

mmon cryog

c liquids are

issue and ca

g precaution

Use and store

Wear approp

ryogenic liqu

ong sleeves,

ryogenic liqu

peration. Co

xcessive ven

n the vessel’

emoved from

All systems co

emperatures

ressure relie

p. Any syste

The officia

ure 5.8 – HF

Liquids Saf

defined as a

enic liquid u

extremely c

n also pose

ns should be

e cryogenic l

priate PPE wh

uids includes

long pants,

uids will ven

ontainers are

nting and/or

s integrity o

m service an

omponents

s.

ef valves mu

em section th


Cylinder Inc

fety

a liquid with

used in a lab

cold. Cryoge

an asphyxia

taken when

liquids in we

hile handling

s chemical s

and closed‐t

nt (boil off) f

e typically of

an isolated

r a problem

nd taken to a

piping, valve

st be in plac

hat could be

43

ocument will only

cident at Pur

a normal bo

oratory sett

nic liquids a

tion hazard

n handling cr

ell ventilated

g cryogenic l

plash goggle

toe shoes.

rom their st

f a vacuum j

ice build‐up

in the proce

a safe, well‐v

es, etc., mus

ce in systems

e valved off w

y be maintained on

rdue Univer

oiling point b

ing is liquid

nd their vap

if handled in

ryogenic liqu

d areas only.

liquids. Prop

es, a face shi

orage conta

jacketed des

p on the vess

ess line. A le

ventilated ar

st be designe

s and piping

while contai

Chapter 5: Labo

nline.

rsity in 2011

below ‐150 °

nitrogen. By

pors can rap

n confined s

uids:

.

per PPE for h

ield, cryogen

ainers as par

sign to minim

sel walls may

eaky contain

rea immedia

ed to withsta

g to prevent

ning cryoge

oratory Manageme

1

°C (‐240 °F).

y definition,

idly freeze

paces. The

handling

nic‐safe glov

t of normal

mize heat los

y indicate a

er should be

ately.

and extreme

pressure bu

nic liquid mu

ent Plan

The

all

ves,

ss.

fault

e

e

uild

ust


44


have a pressure relief valve. The pressure relief valve relief ports must be positioned to

face toward a safe location.

Transfer operations involving open cryogenic containers, such as Dewars must be done

slowly, while wearing all required PPE. Care must be taken not to contact non‐insulated

pipes and system components.

Open transfers will be allowed only in well‐ventilated areas.

Do not use a funnel while transferring cryogenic liquids.

Use tongs or other similar devices to immerse and remove objects from cryogenic

liquids; never immerse any part of your body into a cryogenic liquid.

5.10 Nanoparticle Safety

The American Society of Testing and Materials (ASTM) Committee on Nanotechnology has

defined a nanoparticle as a particle with lengths in two or three dimensions between 1 and 100

nanometers (nm). Nanoparticles can be composed of many different base materials and may be

of different shapes including: nanotubes, nanowires, and crystalline structures such as

fullerenes and quantum dots. Nanoparticles present a unique challenge from an occupational

health perspective as there is a limited amount of toxicological data currently available for

review. However, some studies have shown that existing exposure control technologies have

been effective in reducing exposure to nanoparticles. Refer to the REM webpage for detailed

procedures and guidance regarding the safe handling of nanoparticle.

(http://www.purdue.edu/rem/home/booklets/nanopolicy.pdf)

5.11 Sharps Handling Safety

Sharps are defined as items capable of puncturing, cutting, or abrading the skin such as glass or

plastic pipettes, broken glass, test tubes, petri dishes, razor blades, needles, and syringes with

needles. Sharps are often contaminated with hazardous chemicals and/or infectious agents, so

multiple hazards are often encountered. Employees that routinely work with sharps must be

aware of the risk of being punctured or lacerated. It is important for these employees to take

precautions and properly handle sharps in order to prevent injury and potential disease

transmission. These employees should use appropriate PPE (e.g., puncture‐resistant gloves),

tools, barrier protection, sharps waste containers, and engineering controls to protect

themselves. Refer to the REM webpage for detailed procedures regarding sharps handling and

disposal procedures. (https://www.purdue.edu/rem/home/booklets/sharps.pdf)

5.12 E

5.12.

The f

Befor

Durin

quipment

1 Centrifug

following saf

re centrifuga

Centrifug

Centrifug

the labora

Train each

review th

Use only r

expiration

Check tub

before ea

Make sur

clean.

Examine O

Never ove

Always ca

Always ba

Check tha

and secur

When usi

and move

ng centrifuga

Close lids

stopped.

Do not ex

The opera

and the m

Stop the c

and check

The officia

, Apparatu

ges

fety guidelin

ation:

es must be p

es cannot be

atory.

h operator o

e user manu

rotors comp

n date for ul

bes, bottles,

ach use.

e that the ro

O‐rings and

erfill centrifu

ap tubes bef

alance bucke

at the rotor i

re it.

ng swinging

e freely.

ation:

at all times

xceed safe ro

ator should n

machine app

centrifuge im

k load balan


us, and Ins

es should be

properly inst

e placed in t

on proper op

ual.

patible with t

tracentrifug

and rotors f

otor, tubes,

replace if w

uge tubes (d

ore centrifu

ets, tubes, a

is seated on

bucket roto

during oper

otor speed.

not leave th

ears to be ru

mmediately

ces.

45

ocument will only

strument S

e followed w

talled and o

the hallway o

perating pro

the centrifug

ge rotors.

for cracks an

and spindle

orn, cracked

don't exceed

gation.

nd rotors pr

the drive co

ors, make su

ration. Neve

e centrifuge

unning safel

if an unusua

y be maintained on

Safety

when operat

perated only

of a building

cedures,

ge. Check th

nd deformiti

are dry and

d, or missing

¾ full).

roperly.

orrectly, clos

re that all b

r open a cen

e until full op

y without vi

al condition

Chapter 5: Labo

nline.

ting centrifu

y by trained

g; they must

he

ies

g.

se the lid on

uckets are h

ntrifuge unti

perating spe

ibration.

(noise or vib

oratory Manageme

ges:

personnel.

remain insid

the centrifu

hooked corre

il the rotor h

ed is attaine

bration) beg

ent Plan

de of

uge,

ectly

has

ed

ins

After

5.12.

Stirri

labor

and s

opera

hood

way t

Only

powe

equip

stirrin

opera

failur

consi

5.12.

Labo

baths

oven

less a

unatt

supp

exten

A num

the la

r centrifugat

Allow the

Wear new

Check ins

thorough

Wash han

2 Stirring a

ng and mixin

ratories inclu

shakers. The

ations that a

, and it is im

that prevent

spark‐free in

er stirring an

pment used

ng and mixin

ated for fair

re, electrical

dered.

3 Heating D

ratories com

s, salt baths,

s. Steam hea

are required

tended with

ly of water f

nded period

mber of gen

aboratory. W

The officia

ion:

e centrifuge t

w pair of out

ide of centri

ly if necessa

nds after rem

nd Mixing E

ng devices co

ude stirring m

se devices a

are performe

mportant tha

ts the genera

nduction mo

nd mixing de

for laborato

ng devices, e

ly long perio

overload or

Devices

mmonly use h

, sand baths,

ated devices

because the

assurance t

for steam ge

of time.

eral precaut

When workin


to come to a

ter gloves to

ifuge for pos

ary.

moving glove

quipment

ommonly fo

motors, mag

are typically

ed in a chem

t they be op

ation of elec

otors should

evices or any

ory operation

especially sti

ods without

r blockage of

heating devi

, air baths, h

s are genera

ey do not pr

that their tem

eneration is s

tions need to

ng with heat

46

ocument will only

a complete s

remove rot

ssible spills a

es.

ound in

gnetic stirrer

used in lab

mical fume

perated in a

ctrical sparks

d be used in

y other rotat

ns. Because

rring motor

constant att

f the motion

ices such as

hot‐tube furn

lly preferred

resent shock

mperature w

sufficient pri

o be taken w

ing devices,

y be maintained on

stop before

tor and samp

and leaks, cl

rs,

s.

ting

s and magne

tention, the

n of the stirr

ovens, hot p

naces, hot‐a

d whenever

k or spark ris

will never ex

ior to leavin

when workin

consider th

Chapter 5: Labo

nline.

opening.

ples.

ean centrifu

etic stirrers,

consequenc

ring impeller

plates, heati

air guns, and

temperatur

sks and can b

xceed 100 °C

g the reactio

ng with heat

e following:

oratory Manageme

uge and roto

are often

ces of stirrer

r should be

ing mantles,

d microwave

es of 100 °C

be left

C. Ensure the

on for any

ing devices

ent Plan

or

r

, oil

C or

e

in

5.12.

The p

dang

perso

distill

chlor

benze

follow

cond

The actua

such a fas

accidenta

If a heatin

exposed,

The exter

ventilatio

cannot be

liquids or

Fail‐safe d

a reaction

loss of rea

power if t

flow of co

pressure

4 Distillatio

process of th

erous. If not

onnel exposu

led in labora

ride, diethyl

ene, and hex

wed while th

ucted in the

The therm

hood if po

Ensure th

Ensure th

Inspect al

Keep all a

consisten

the fume

The officia

al heating ele

shion as to p

ally touching

ng device be

the device s

rnal cases of

n and, there

e spilled ont

vapors.

devices can

n increases s

action solve

the tempera

ooling water

or loosening

on and Solve

hermal solve

t handled pr

ure can resu

atories includ

ether, tolue

xanes. The g

hermal distil

e lab:

mal solvent d

ossible.

at all heat g

at all water

ll glassware

air and wate

t efforts to n

hood where


ement in any

prevent a lab

g the wire ca

ecomes so w

should be eit

all variable

efore, should

o them and

prevent fires

significantly

nt, or loss of

ature of the h

r through a c

g of the wate

ent Purificat

ent distillatio

operly, fire,

ult. A few com

de tetrahydr

ne, dimethy

guidelines be

lation of org

distillation sy

generating eq

connections

for defects b

r‐sensitive d

not store or

e distillation

47

ocument will only

y laboratory

boratory wor

rrying the el

orn or dama

ther discard

autotransfo

d be located

where they

s or explosio

because of a

f cooling. So

heating devi

condenser is

er supply ho

ion Systems

on is inheren

explosion, a

mmon chem

rofuran, met

ylformamide

elow should

ganic solvent

ystem shoul

quipment ha

s on the con

before settin

drying agents

use other fl

is taking pla

y be maintained on

y heating dev

rker or any m

lectric curre

aged that its

ed or repair

ormers have

d where wate

will not be e

ons that may

a change in

ome devices

ice exceeds

stopped ow

ose to a cond

s

ntly

and/or

micals

thylene

e,

be

ts is

d be installe

as a shut‐off

ndenser are c

ng them up i

s under iner

ammable or

ace.

Chapter 5: Labo

nline.

vice should

metallic con

ent.

s heating ele

red before it

perforation

er and other

exposed to f

y arise if the

line voltage,

will turn off

some prese

wing to the lo

denser.

ed inside of a

f device insta

clamped sec

in the exper

rt atmosphe

r hazardous

oratory Manageme

be enclosed

ductor from

ement is

is used agai

s for cooling

r chemicals

flammable

e temperatur

, the acciden

f the electric

t limit or if t

oss of water

a chemical f

alled.

curely.

riment.

re. Make

chemicals in

ent Plan

d in

m

in.

g by

re of

ntal

c

the

r

fume

nside

5.12.

Broke

cuts t

risk o

5.12.

Work

explo

press

The f

the la

5 Laborator

en laborator

to multiple s

of accidents,

Temperat

glassware

temperat

Only roun

specifical

Inspect gl

using. Som

the glassw

there is a

is fine, wh

present.

Don’t kee

graduated

dispose o

Always w

temperat

6 High Pres

king with hig

osion, and th

sure systems

following pro

aboratory:

High‐pres

appropria

protected

Vessels, c

the stress

must not

All pressu

The officia

ry Glasswar

ry glassware

stitches and

the followin

ture changes

e with cold w

ures.

nd‐bottomed

ly designed f

assware for

metimes a h

ware with a

defect. A rin

hile a dull “th

ep cracked g

d cylinder is

f it.

ear appropr

ures. Always

ssure System

gh pressure s

he possible h

s must be se

ocedures sho

ssure operat

ately selecte

d by pressure

connecting h

ses encounte

corrode or o

ure equipme


e

is dangerou

eye damage

ng guideline

s can shatte

water, espec

d or thick‐wa

for operatio

any small im

airline crack

pen and liste

nging tone in

hud” indicat

lassware. If t

chipped or

riate PPE wh

s wear safet

ms

systems in a

hazards of fly

et up and ope

ould be follo

tions should

d for the op

e‐relief cont

hoses, and an

ered at the i

otherwise re

nt should be

48

ocument will only

us. Glasswar

e are commo

s should be

r any labora

ially after au

alled (e.g., P

ons at reduce

mperfection

k may be pre

en to the to

ndicates the

tes there is a

the bottom

broken, pro

en working

ty glasses.

laboratory

ying glass, ch

erated with

owed when w

be perform

eration, pro

trol devices.

ny apparatu

ntended op

eact when in

e visually ins

y be maintained on

re‐related inj

on to lab wo

followed:

atory glasswa

utoclaving o

Pyrex) evacu

ed pressure

s before

esent. Tap

ne to tell if

e glassware

a flaw

of a

perly

with glassw

can result in

hemical exp

careful cons

working wit

ed only in p

operly labele

s must be st

erating tem

n contact wit

spected befo

Chapter 5: Labo

nline.

juries rangin

orkers. In ord

are. Never fl

r exposure t

ated reactio

should be u

are and vary

n over‐press

osure, and f

sideration o

h high press

ressure vess

ed and instal

trong enoug

peratures an

th the mater

ore each use

oratory Manageme

ng from sma

der to reduc

lash‐cool

to any high

on vessels

sed.

ying

urization,

fire. All high

f potential r

sure systems

sels

led, and

h to withsta

nd pressures

rials it conta

e.

ent Plan

ll

ce the

risks.

s in

and

s and

ains.

5.12.

Vacu

chem

consi

vacuu

7 Vacuum S

um work can

micals, and fi

deration of

um apparatu

Do not all

Protect p

Assemble

of the flas

Avoid put

glass appa

Place vac

possibility

necessary

prevent in

explosion

When usi

compone

made of P

should be

guard aga

speed and

should be

When usi

experime

pump and

people w

related od

following

o M

o M

lin

o To

th

de

The officia

Systems

n result in an

re. All vacuu

the potentia

us in the lab

low water, s

umps with c

e vacuum ap

sk.

tting pressur

aratus from

uum appara

y of being ac

y, place tran

njury from fl

n.

ng a rotary e

nts of the ro

Pyrex or sim

e completely

ainst flying g

d application

e gradual.

ng a vacuum

ntal apparat

d the piping

ho must wo

dors from be

vacuum tra

ake sure the

ake sure the

ne.

o prevent co

e vacuum so

evice as appr


n implosion

um operation

al risks. The

oratory:

solvents, or c

cold traps an

paratus in a

re on a vacu

exploding.

tus in such a

ccidentally h

sparent plas

lying glass in

evaporator,

otary evapor

ilar glass. Gl

y enclosed in

glass should t

n of vacuum

m source, it i

tus and the v

from the po

rk on the va

eing emitted

pping guide

e flask is pro

e vacuum ho

ntamination

ource should

ropriate.

49

ocument will only

and the pos

ns must be s

following gu

corrosive ga

nd vent their

manner tha

um line to p

a way that th

it is minimiz

stic around i

n case of an

the glass

rator should

ass vessels

n a shield to

the compon

to the flask

s important

vacuum sou

otentially da

cuum lines o

d back into t

lines should

operly clamp

ose is connec

n, all lines lea

d be equippe

y be maintained on

ssible hazard

set up and o

uidelines sho

ses to be dra

r exhaust int

at avoids stra

prevent stop

he

zed. If

t to

be

nents implod

k whose solv

to place a t

rce. The vac

maging effe

or system, a

he laborato

be followed

ped and secu

cted to the v

ading from e

ed with filtra

Chapter 5: Labo

nline.

ds of flying g

operated wit

ould be follo

awn into vac

to an exhaus

ain, particul

cocks from p

de. Increase

vent is to be

rap between

cuum trap pr

cts of the m

nd prevents

ry or system

d:

ured.

vacuum line

experimenta

ation or othe

oratory Manageme

glass, splatte

th careful

owed when u

cuum system

st hood.

arly to the n

popping out

in rotation

evaporated

n the

rotects the

material, prot

s vapors and

m exhaust. Th

, not the gas

al apparatus

er trapping

ent Plan

ering

using

ms.

neck

t or

tects

d

he

s

s to

5.13 R

Research

Samples

create un

requirem

A

St

te

co

b

A

n

a

la

is

sa

as

Sa

St

lo

h

Sa

th

to

If

d

o Fo

th

o Fo

ad

o Fo

co

of

o Fo

sc

Research Sa

h samples an

often accum

nsafe and no

ments apply t

All samples m

torage in be

emporarily s

ork, Parafilm

e used.

All samples m

ame, date th

nd the name

abeling syste

s not accepta

amples. Acro

s long as an

amples shou

tockpiling un

onger necess

azardous wa

amples mus

he best of yo

oxicity.

f the hazard(

etermine wh

The officia

or particulate

e size range

or most aque

dequate to p

or solvents a

old enough t

f collecting fl

or highly rea

rubbing dev

amples an

nd chemicals

mulate in lab

on‐complian

to samples d

must be kept

akers or flas

storing samp

m®, or some

must be labe

he sample w

e of generat

em that is on

able as the o

onyms can b

up‐to‐date l

uld be dispos

nusable sam

sary must be

aste program

t be stored a

our ability co

(s) of a samp

hether it is h


es, use filtra

e being gene

eous or non‐

prevent liqui

nd other vo

o condense

luid that cou

ctive, corros

vice capable

nd Chemica

s developed

bs for years a

t conditions

developed in

t closed exce

sks should b

ples in beake

other closur

led with the

was develope

or. Chemica

nly known to

only means o

be used as a

legend is po

sed of within

mples in not a

e properly d

m.

according to

onsidering th

ple are unkn

hazardous or

50

ocument will only

tion capable

rated.

‐volatile liqu

ds from gett

latile liquids

vapors gene

uld be aspira

sive, or toxic

of trapping

als Develo

in the lab (s

and are diffic

s if not mana

n the laborat

ept when in

e temporary

ers or flasks,

re device mu

e chemical

ed/received,

al structure o

o lab person

of labeling

labeling syst

sted in the l

n 6 months

an acceptab

isposed of in

o the primary

he propertie

own, the La

r not. Assum

y be maintained on

e of efficient

uids, a filter f

ting to the v

s, use a cold

erated, follo

ated out of t

c gases, use a

the gas.

ped in the

samples) mu

cult to ident

aged proper

tory:

use.

y. If

a

ust

,

or a

nel

tem

ab.

unless active

le practice. A

n a timely m

y hazard cla

es that are kn

boratory Su

me all sample

Chapter 5: Labo

nline.

tly trapping

flask at room

vacuum sour

trap of suffi

owed by a filt

the cold trap

a sorbent ca

e Lab

ust be manag

tify and disp

ly. The follow

ely being us

All samples

manner using

ss; this shou

nown or ass

pervisor mu

es are toxic u

oratory Manageme

the particles

m temperatu

rce.

icient size an

ter flask cap

p.

anister or

ged respons

ose of and c

wing

ed for analy

that are no

g REM’s

uld be done t

sumed such

ust attempt t

unless other

ent Plan

s in

ure is

nd

pable

ibly.

can

ysis.

to

as

to

rwise

d

o

d

(7

If

la

th

to

P

F

te

re

If

S

re

5.14 T

Transpor

accidents

chemical

Purdue‐o

emonstrate

f other simil

etermine if a

765) 49‐4012

f samples are

abel every sa

he same haz

oxic) can hav

harmaceutic

igure 5.9 can

emporary ba

esponders, a

f the chemic

upervisor m

equirements

F

Transportin

rting chemic

s and potent

l off campus

owned vehic

The officia

d. This can b

lar compoun

a chemical is

21 for assist

e consolidat

ample conta

zard class (e.

ve one label

cal Compoun

n be used to

asis. This typ

as well as giv

al substance

ust comply w

s for prepara

igure 5.9 – E

ng Hazardo

als is a pote

tial injuries.

using a ship

cle, and how


be accomplis

nds. At a min

s flammable

ance with id

ed for storag

iner. For exa

g., miscellan

on the outs

nds” or a sim

o identify con

pe of informa

ves REM the

e is produced

with the Haz

ation of SDSs

Example Tem

ous Chemi

ntially hazar

The followin

pping compa

w to safely m

51

ocument will only

shed by liter

nimum, the L

e, corrosive,

dentifying th

ge (e.g., vial

ample, a box

neous pharm

side of the bo

milar descrip

nsolidated sa

ation comm

information

d for anothe

zard Commu

s and contai

mporary Sam

cals

rdous proces

ng subsectio

any, how to t

ove chemica

y be maintained on

rature review

Laboratory S

oxidant, or

e hazards of

boxes), it is

x containing

maceutical co

ox stating “M

ption. A labe

amples, and

unicates the

n necessary

er user outsi

unication Sta

iner labeling

mple Contai

ss that must

ons discuss h

transport ch

als by foot a

Chapter 5: Labo

nline.

w or reviewi

Supervisor s

reactive. Ca

f samples.

s not always

g sample vial

ompounds c

Miscellaneo

l such as the

d should only

e hazards to

for proper d

de of the lab

andard inclu

g.

ner Label

t be done pr

how to prope

hemicals on

cross campu

oratory Manageme

ng the haza

hould be ab

ll REM at (76

necessary to

s which are

considered t

us Toxic

e one shown

y be used on

emergency

disposal.

b, the Labor

uding the

roperly to av

erly ship

campus usin

us.

ent Plan

rds

ble to

65)

o

all in

to be

n in

n a

atory

void

ng a


52


5.14.1 Shipping Hazardous Chemicals off Campus

Shipping chemicals, research samples, or other similar materials off campus is potentially

regulated by the Department of Transportation (DOT) and/or other regulatory agencies.

Chemicals regulated for shipping require very specific types of packaging, labeling, and

documentation and must be prepared by trained personnel. REM makes the determination

on whether a chemical is classified as hazardous for transportation purposes. Unless the

researcher is DOT trained, they are not authorized to make this determination. Shipments

that are not prepared by trained personnel can result in delays, loss of research samples,

and potential regulatory fines. REM can provide assistance by either providing shipment

services, or if necessary, training personnel on the proper shipping procedures. More

information about shipping chemicals can be found on the REM webpage.

(http://www.purdue.edu/rem/hmm/shiphm.htm)

As previously stated, REM prepares all regulated chemicals for shipment according to DOT

requirements. However, laboratory personnel prepare the inner container (e.g., vial, jar)

and provide it to REM for shipment. When selecting an inner container to be given to REM

for an off‐campus hazardous materials shipment, the following guidelines must be followed:

The chemical must be compatible with the container. For example, corrosive

chemicals must not be placed in metal containers; hydrofluoric acid in any

concentration must not be placed in glass containers.

Chemical permeability should be considered when selecting a plastic container,

especially for organic solvents. The container must be able to effectively contain the

chemical during transportation under normal conditions.

The container must have an appropriate lid that is able to close and seal, meaning

the container will not leak during transportation under normal conditions. Any

containers that do not properly seal (e.g., beaker, flask, test tube) will not be

shipped off campus by REM.

5.14.2 Transporting Chemicals on Campus via Purdue Vehicle

Purdue University is a state agency and therefore is exempt from Department of

Transportation (DOT) hazardous materials regulations. However, the “intent” of the DOT

regulations is still required when transporting chemicals on campus using a motor vehicle.

This essentially means that all chemical containers must be properly packaged, labeled, and

segregated according to hazard class. Do not attempt to move large volumes (e.g., greater

than 5 gallons in total volume) of chemicals across campus. If a large volume of chemicals

needs to be moved across campus, such as an entire lab move, contact REM (765) 49‐40121


53


for further assistance. The following procedures must be followed in order to properly and

legally transport chemicals across campus:

Only Purdue‐owned vehicles are permitted to be used to transport chemicals. For

liability and insurance purposes, no personal vehicles should ever be used to

transport hazardous chemicals.

Ensure that each container has an appropriate, tight fitting lid. The lid should have

the ability to contain the contents of the container even if it becomes inverted

during transport. Examples of inappropriate lids include cracked caps, loosely fitting

rubber stoppers, or Parafilm®.

Chemicals should be segregated according to the primary hazard class. For example,

do not place an oxidizer such as ammonium nitrate in the same container as an

organic solvent such as acetone.

All containers should be packaged upright.

Chemical containers should be placed in some type of outer packing such as a box,

bin or bucket. Containers should remain securely packaged during loading,

transport, and unloading. Glass to glass contact should be avoided. Bubble wrap,

newspaper, and vermiculite are good examples of packaging material that will

prevent glass to glass contact.

The outer containers should remain tightly secured during transport. Measures

should be taken to avoid movement of the outer containers. For example, the

containers should be secured using a strap or an empty box can be used to fill the

gap between the last box and the sidewall of the vehicle.

The outer container must be labeled in a manner that identifies the contents (e.g.

corrosives, flammables).

Transport with two or more people if possible.

Be prepared for unseen accidents. At least one person should be knowledgeable of

the materials being transported. An inventory with an estimated volume or weight

per hazard should be recorded and available during transport (e.g., 5 gallons of

flammable liquid and 10 pounds of toxic solids).

Prepare a spill kit prior to transport. Material such as appropriate PPE, absorbent

material, and an empty bucket is sufficient for most small spills.

Carry a cell phone and know who to call in the event of an emergency. The Purdue

Fire Department will respond to on‐campus emergencies. Dial 911 from a Purdue

phone or (765) 49‐48221 from a cell phone to contact Purdue Police dispatch.

5.14.

Trans

camp

volum

a larg

(765)

movi

3 Transport

sporting sma

pus buildings

mes (e.g., gre

ge volume of

) 49‐40121 fo

ng chemical

PPE must

time whic

or even a

public are

brought a

Purdue S

breakable

customer

container

transport

Room. Ch

purchase

Purdue St

container

bottle car

plastic wi

enough to

container

covers are

acids/alka

bottle car

Laborator

stable and

Plan the r

Freight el

chemicals

should op

person is

The officia

ting Chemic

all volumes o

s) is accepta

eater than 5

f chemicals n

or further as

s on campus

be worn wh

ch the chem

ppropriate (

eas). Howeve

along in the e

Stores’ stock

e containers

r has an appr

r in which to

ing before le

hemical requ

a transport

tores. Appro

r means a co

rrier made o

th carrying h

o hold the co

r if broken in

e recommen

alis; and met

rrier is illustr

ry carts used

d in good co

route ahead

evators, not

s whenever

perate the el

transporting


als on Camp

of chemicals

ble as long a

5 gallons in t

needs to be

ssistance. Th

s by way of f

hen handling

icals are mo

(e.g., employ

er, appropri

event of a sp

room perso

of any size u

roved transp

place the ch

eaving the S

uisitioners m

container fr

oved transpo

ommercially

of rubber, me

handle(s) wh

ontents of th

n transit. Car

nded, but no

tal, rubber, o

rated in Figu

d to transpor

ondition. Tra

of time so a

t passenger

possible. Th

levator alon

g hazardous

54

ocument will only

pus via Foot

s across cam

as it is done

otal volume

moved, suc

he following

foot:

g potentially

oved on cam

yees should

ate PPE and

pill or incide

onnel shall n

unless the

port

hemical for

tock

may

rom

ort

available

etal, or

hich is large

he

rrier lids or

ot required.

or plastic for

ure 5.10.

rt chemicals

nsport only

as to avoid a

elevators, sh

e individual

e if possible

chemicals.

y be maintained on

t

mpus via foot

properly. Do

e) of chemica

h as an enti

procedures

y contaminat

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not wear ch

spill contain

ent.

ot dispense

Rubber or p

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transporting

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Figure 5.

Chapter 5: Labo

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t (e.g., from

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hemical‐resis

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10 – Chemic

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arge

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n

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sary

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ld be

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us

cals

n a

arrier


55


5.15 Laboratory Security

All laboratory personnel have a responsibility to protect university property from misuse and

theft of hazardous materials, particularly those that could threaten human health. At a

minimum, the following security measures should be employed in all campus laboratories:

The laboratory door should remain locked when not occupied.

Always feel free to question anyone that enters the lab that you do not know and ask to

see identification if necessary.

If you see anything suspicious or someone displays suspicious behavior, immediately

report it to the Purdue Police Department by dialing 911 (emergency) or (765) 494‐8221

(non‐emergency).

Any sensitive information or particularly hazardous chemicals should not be stored out

in the open where anyone can readily have access to them. These types of materials

should be stored in a secure location and lab personnel should always be present when

these materials are in use.

5.16 Laboratory Self‐Inspections

REM performs laboratory inspections for various purposes (e.g., routine building safety and

compliance inspections). However, the Laboratory Supervisor a qualified designee should also

inspect the laboratory for compliance with the requirements of the CHP at a minimum on an

annual basis. Lab personnel have a much greater understanding of the unique hazards and

issues that are encountered in their individual lab than REM does. The goal of these inspections

is to identify and correct unsafe and non‐compliant conditions that could potentially result in an

injury to lab personnel or a fine from a regulatory agency (e.g., open hazardous waste

container). All deficiencies found during the inspection should be reviewed and corrected. The

following elements should be performed during these inspections:

Housekeeping practices should be reviewed. Chemicals should be stored appropriately

and labeled. Evidence of spills and/or chemical contamination should be cleaned. All

glassware and equipment should be stored appropriately, etc.

Hazard assessments should be updated if process changes have occurred. For example,

the lab is now performing organic synthesis and working with organometallic

compounds.

Training records should be updated and documented if new lab personnel have not yet

been trained or if any processes have changed.

Excess or outdated chemicals should be properly disposed of by REM.

Sa

n

A recomm

Checklist

(https://w

5.17 L

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57


Extension cords may only be used to provide temporary power and must be used

with Ground Fault Circuit Interrupter (GFCI) protection during maintenance and

construction activities and in damp or wet locations.

Portable cord and plug connected equipment and extension cords must be visually

inspected before use for external defects such as loose parts, deformed and missing

pins, or damage to outer jacket or insulation, and for possible internal damage such

as pinched or crushed outer jacket. Any defective cord or cord‐and‐plug‐connected

equipment must be removed from service and no person may use it until it is

repaired and tested to ensure it is safe for use.

Extension cords must be of the three‐wire type. Extension cords and flexible cords

must be designed for hard or extra hard usage. The rating or approval must be

visible.

Portable equipment must be handled in a manner that will not cause damage.

Flexible electric cords connected to equipment may not be used for raising or

lowering the equipment.

Extension cords must be protected from damage. Sharp corners and projections

must be avoided. Flexible cords may not be run through windows or doors unless

protected from damage, and then only on a temporary basis. Flexible cords may not

be run above ceilings or inside or through walls, ceilings or floors, and may not be

fastened with staples or otherwise hung in such a fashion as to damage the outer

jacket or insulation.

Extension cords used with grounding type equipment must contain an equipment‐

grounding conductor; the cord must accept a three‐prong, or grounded, plug.

Operating equipment with extension cords without a grounding plug is prohibited.

Attachment plugs and receptacles may not be connected or altered in any way that

would interrupt the continuity of the equipment grounding conductor. Additionally,

these devices may not be altered to allow the grounding pole to be inserted into

current connector slots. Clipping the grounding prong from an electrical plug is

prohibited.

Flexible cords may only be plugged into grounded receptacles. Adapters that

interrupt the continuity of the equipment grounding connection may not be used.

All portable electric equipment and flexible cords used in highly conductive work

locations, such as those with water or other conductive liquids, or in places where

employees are likely to contact water or conductive liquids, must be approved for

those locations.

Employee's hands must be dry when plugging and unplugging flexible cords and cord

and plug connected equipment if energized equipment is involved.

5.18.

Temp

cords

main

If the con

cord conn

receptacl

Lamps for

sockets m

Temporar

designed

Extension

the sectio

Temp3

porary electr

s, cables and

tenance, rep

Ground‐fa

circuits, in

In genera

Listed or

Receptac

Flexible c

and inten

run throu

or similar

building w

Suitable d

disconnec

Lamps for

damage, e

working s

cord must

material a

Flexible c

and proje

damage w

The officia

nection cou

nector is wet

e connection

r general illu

must be grou

ry lights mus

for this purp

n cords are c

on on “Requ

porary Wirin

rical power a

d extension c

pair, or expe

ault protecti

ncluding ext

l, all equipm

labeled dou

les must be

ords and cab

ded use. Th

ugh holes in w

openings, w

walls, ceiling

disconnectin

ction of all u

r general illu

either by ele

surface or by

t be equippe

and must be

ords and cab

ections are to

when they p


ld provide a

t from being

ns must be h

umination m

nded.

st not be sus

pose.

onsidered to

irements for

g Requirem

and lighting

cords, may o

erimental wo

ion (e.g. GFC

ension cords

ment and too

ble insulated

of the groun

bles must be

ey may not

walls, ceiling

where attach

gs, floors, rug

ng switches o

ungrounded

umination m

evating the f

y providing a

ed with a ha

e equipped w

bles must be

o be avoided

ass through

58

ocument will only

conducting

g immersed i

handled only

ust be prote

spended by t

o be tempor

r Temporary

ents

installations

only be used

ork. The follo

CI) must be p

s, used for c

ols connecte

d tools and a

nding type.

e of an appro

be used as a

gs or floors,

hed to buildi

gs or carpeti

or plug conn

conductors

ust be prote

fixture above

a suitable gu

ndle of mold

with a substa

e protected f

d. Flexible co

doorways o

y be maintained on

g path to the

in water), th

y with insula

ected from b

their cords u

rary wiring, a

y Wiring” in t

s 600 volts o

d during and

owing additi

provided on

construction

d by cord an

appliances n

oved type an

a substitute

where run t

ing surfaces,

ing .

nects must b

of each tem

ected from a

e 8 feet abo

uard. Hand la

ded compos

antial bulb g

from accide

ords and cab

or other pinc

Chapter 5: Labo

nline.

e employee’s

he energized

ating protect

breakage, an

unless they h

and must als

this program

or less, includ

for renovat

ional require

all tempora

or mainten

nd plug must

need not be

nd suitable f

for the fixed

through doo

, or where c

e installed t

mporary circu

accidental co

ve the floor

amps supplie

sition or othe

guard.

ntal damage

bles must be

ch points.

oratory Manageme

s hands (e.g.

d plug and

tive equipm

nd metal she

have been

so comply w

m.

ding flexible

ion,

ements appl

ary‐wiring

ance activiti

t be grounde

grounded.

for the locat

d wiring, whe

rways, wind

oncealed be

o permit the

uit.

ontact or

or other

ed by flexibl

er approved

e. Sharp corn

e protected f

ent Plan

. if a

ent.

ell

with

e

ly:

ies.

ed.

tion

ere

dows

ehind

e

e

d

ners

from

5.18.

Work

shou

until

while

Wet or Da4

k in wet or d

ld not be pe

the liquid ca

e performing

Only use e

Place a dr

Remove s

there is st

Do not us

Keep elec

The officia

amp Locatio

amp work lo

rformed unl

an be cleane

g work in dam

electrical co

ry barrier ov

standing wat

tanding wate

se electrical

ctrical cords


ons

ocations (i.e

less it is abso

ed up. The fo

mp location

rds that hav

ver any wet o

ter before b

er;

extension co

away from s

59

ocument will only

., areas surro

olutely critic

ollowing spe

s:

ve GFCIs;

or damp wo

eginning wo

ords in wet o

standing wat

y be maintained on

ounded or n

cal. Electrica

cial precaut

rk surface;

ork. Work is

or damp loca

ter.

Chapter 5: Labo

nline.

near water o

l work shou

ions must be

prohibited i

ations; and

oratory Manageme

or other liqui

ld be postpo

e incorporat

n areas whe

ent Plan

ids)

oned

ted

ere

Chapt

6.1 P

The purp

Purdue la

protect t

has been

1910.132

should ne

6.2 S

This Labo

chemical

encompa

Lafayette

facilities

confined

laborato

Purdue U

REM at (7

(http://w

6.3 H

The haza

and pres

minimize

certificat

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readily av

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time a pr

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hemselves a

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ever be used

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765) 49‐467

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Hazard Ass

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that all lab

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rocess is mo

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Laboratory P

es are aware

against chem

n accordance

910.140, 29

d in place of

Policy applie

afety and he

ue PPE Policy

egional camp

ons. This Lab

y, welding op

rs hazards n

ersonal Prote

31 for assist

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essment

ent is a proce

along with o

m the hazar

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for defined t

ents are per

posted in eac

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hazard asses

dified or wh


ry PPE P

Personal Pro

e of the PPE

mical, radiolo

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CFR 1910.95

f engineering

es to all pers

ealth hazards

y that applie

puses, resea

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perations, an

not covered

ective Equip

tance.

home/bookle

ess of identi

other relevan

rds. Hazard a

nt, which is

tasks. The La

rformed and

ch location. T

eceive docum

sment shou

hen a new ta

60

ocument will only

Policy

tective Equi

requiremen

ogical, biolog

equirements

5). As briefly

g and admin

sonnel that w

s. This policy

es to all area

arch farms a

E Policy doe

nd high volta

in this Labor

pment (PPE)

ets/PPEPolic

ifying the ha

nt protection

assessments

a written do

aboratory Su

the certifica

The Laborat

mented train

ld be review

sk which pre

y be maintained on

pment (PPE

nts and proce

gical, or mec

s of the OSH

y discussed i

nistrative con

work with or

y is a part of

s (not just la

nd agricultu

s not cover

age) in all op

ratory PPE P

Policy for m

cy.pdf)

azards assoc

n measures

s are perform

ocument det

upervisor is

ation(s) is w

tory Supervis

ning on app

wed at least a

esents a haz

Chapte

nline.

) Policy is to

edures to ad

chanical haz

HA PPE regul

n Chapter 4

ntrols.

r around haz

f the larger,

aboratories)

ural centers a

all potential

perations or

Policy, then r

more informa

iated with a

that must b

med by com

tailing the ha

responsible

written, signe

sor is also re

licable haza

annually and

zard is introd

r 6: Laboratory PP

o ensure that

dequately

zards. This po

ations (29 C

of the CHP,

zardous

all‐

of the West

and related

l hazards (e.

r settings. If a

refer to the

ation or cont

defined tas

be employed

pleting a

azard

for ensuring

ed, dated, an

esponsible fo

rd assessme

d updated an

duced into th

PE Policy

t all

olicy

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nd

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ents.

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Chapter 6: Laboratory PPE Policy

61


Hazard assessments can be organized using three formats: by individual task (e.g., pipetting

hazardous liquids), by location (e.g., Chemistry Laboratory Room 1250), or by job title (e.g.,

Chemistry Lab Technician). Any of these formats is acceptable and often will be used in

conjunction with each other to provide the safest laboratory work environment possible for

employees. The following subsections describe each hazard assessment format in more detail.

6.3.1 Task Evaluation Hazard Assessment

Task evaluation hazard assessments should be conducted for specific tasks such as

preparing dilute hydrochloric acid solutions or an ozonolysis reaction and workup. These

types of hazard assessments should be written in a very detailed manner. The following

describes the steps that should be taken to perform a task evaluation hazard assessment:

Describe the task.

List hazards associated with each body part.

Determine PPE requirements for each hazard.

List other control measures required such as engineering and administrative

controls.

See the REM webpage for the task evaluation hazard assessment template.

(http://www.purdue.edu/rem/home/files/forms.htm#ppe)

6.3.2 Location Evaluation Hazard Assessment

Location evaluation hazard assessments should be conducted for specific

areas/laboratories. These types of hazard assessments should be written in a

comprehensive manner that includes the majority of hazards present in a specific location

(e.g., flammable and corrosive liquids). This type of hazard assessment is the most

commonly used in laboratories and should be posted in a location within the lab where it is

easily accessed by personnel (e.g., posted near the front door of the lab). If employees

perform specific tasks not covered by the laboratory hazard assessment, then it will be

necessary to perform another type of hazard assessment such as the task evaluation

assessment that does address the specific hazards of that task. The following describes the

steps that should be taken to perform a task evaluation hazard assessment:

Identify the hazards.

List each task where hazard is present.

Determine PPE requirements for each task.

List other control measures required engineering and administrative controls.


62


See REM webpage for the location evaluation hazard assessment template.


6.3.3 Job Title Evaluation Hazard Assessment

Job title evaluation hazard assessments should be conducted for specific positions. These

types of hazard assessments should be written in a comprehensive manner that includes

the majority of hazards that a specific job position (e.g., Animal Care Technician) routinely

encounters during the normal course of work. This type of hazard assessment is commonly

used for positions where the hazards encountered do not frequently change. If the

employee encounters a hazard that is not covered by the job title evaluation hazard

assessment, then it will be necessary to perform another type of hazard assessment such as

the task evaluation hazard assessment that does address the specific hazards of that task.

The following describes the steps that should be taken to perform a task evaluation hazard

assessment:

Identify hazards that the position title may encounter while performing normal

duties.

List each task where hazard is present.

Determine PPE requirements for each task.

List other control measures required.

See the REM webpage for the job title evaluation hazard assessment template.


6.4 Minimum PPE Requirements for Laboratories

This section details the minimum PPE requirements for all laboratories using hazardous

chemicals. These requirements do not apply to labs that involve solely mechanical, computer,

laser or other non‐ionizing radiation, or electrical operations. The requirements listed do not

cover all operations in all laboratories. Some operations and procedures may warrant further

PPE, as indicated by the SDS, the SOP for the chemical(s) being used, facility policies, or

regulatory requirements. Figure 6.1 illustrates the minimum PPE required when using

hazardous chemicals in a laboratory.

6.4.1

If the

worn

there

6.4.2

Heari

exces

diffic

noise

Head Pro

ere is a serio

n. Each affect

e is a potenti

Hearing P

ing protectio

ssively noisy

ult to comm

e level evalua

The officia

Figure 6.

tection

us risk of ch

ted employe

ial for injury

Protection

on is not typ

y (e.g., opera

municate with

ation.


.1 – Approp

emical splas

ee must wea

y to the head

pically requir

ating equipm

h co‐worker

63

ocument will only

riate PPE fo

sh to the hea

ar protective

d from falling

red in labora

ment that is l

rs while in th

y be maintained on

r the Labora

ad, a chemic

e helmets wh

g objects or

atory setting

oud, air han

he lab, conta

Clos

Lon

CheGlo

Lab

Safe

or G

Chapte

nline.

atory

cal‐resistant

hen working

“bump” haz

gs. However,

ndling unit is

act REM (765

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ng Pants

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r 6: Laboratory PP

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PE Policy

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6.4.3

The u

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(http

6.4.4

Each

face p

from

that m

worn

labor

shield

trans

prote

appro

lab if

(e.g.,

opera

6.4.5

Each

when

chem

extre

Respirato

use of respir

ving hazardo

ble. When v

rd, respirato

ective equipm

ble hazards.

ection factor

piratory Prot

proper equip

pment users

spirator use

ired. Contac

ram or visit t

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4 Eye and F

affected em

protection e

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the employ

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ations).

Hand Pro

affected em

n the hands

micals, cuts, a

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The officia

ory Protectio

ators in the

ous material

ventilation is

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. Respirator

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tection Prog

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concerning

rs when req

t REM at (76

the REM we

rdue.edu/rem

Face Protect

mployee mus

equipment w

plash, flying d

n the laborat

by all indivi

Splash‐proo

ore appropri

ardous liquid

ment must b

he work bei

yee is sitting

a desktop co

otection

mployee mus

may be expo

abrasions, p

cal‐resistant


on

laboratory s

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selection is

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gram”. This p

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the method

uired, and m

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tion

st use appro

when expose

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tory. Safety

duals that a

f safety gogg

iate depend

ds outside of

be American

ng done. Eye

at a worksta

omputer, ha

st wear appr

osed to skin

unctures, or

t gloves mus

64

ocument will only

setting is not

onducted in

ate to provid

nt may be ne

but no one d

based on th

are not to b

program incl

e job, trainin

ds for proper

medical surve

1 with quest

ooklets/RPP9

priate eye a

ed to hazards

ther exposur

glasses mus

re occupying

gles and/or

ing on the ty

f a chemical

n National St

e and face p

ation or desk

aving a lab m

ropriate han

contact of h

r harmful tem

st be worn w

y be maintained on

t typically ne

a chemical

de protection

ecessary. The

device will p

e chemical a

be used exce

ludes a revie

ng of all resp

r use and ca

eillance of re

tions about t

98.pdf)

nd

s

res

st be

g the

a face

ype of work

fume hood

tandards Ins

protection m

k that is awa

meeting at a t

d protection

hazardous

mperature

while handlin

Chapte

nline.

ecessary sin

fume hood w

n against an

ere is a varie

provide prote

and process

ept in conjun

ew of the pro

piratory prot

are of such e

espirator us

the Respirat

being perfo

or glove box

stitute (ANSI

may not be re

ay from chem

table away f

n

ng any

r 6: Laboratory PP

ce all work

whenever

n inhalation

ety of respira

ection again

hazard, and

nction REM’s

ocess to ens

tective

quipment, f

ers when

tory Protecti

ormed (e.g.,

x). All eye

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equired in th

mical proces

from hazard

PE Policy

atory

st all

d the

s

sure

fitting

ion

and

he

sses

dous

hazar

shou

appro

speci

hazar

glove

Do no

chart

(nitri

chem

gene

such

heat‐

The v

well.

solut

prote

a bas

shou

Chem

eleva

prior

fount

6.4.6

Each

body

at ris

skirt

the la

apron

or ad

chem

trans

sized

recom

rdous chemi

ld be assum

opriate glove

fic hazardou

rdous chemi

e manufactu

ot purchase

t. It is recom

le gloves are

micals than la

ral chemical

as liquid nit

‐resistant glo

volume of ha

For example

ion, disposa

ection. But if

se bath for in

ld be selecte

mical‐resistan

ators, offices

to handling

tains, teleph

Body Prot

affected em

from recog

k of injury sh

must be wo

aboratory ar

ns, or protec

djacent to, al

mical bottle is

sferred, pipe

for the indiv

mmended) t

The officia

ical containe

ed that all c

es, it is impo

us chemical

icals than ot

rer’s glove c

gloves from

mended tha

e typically m

atex gloves d

l handling. W

rogen or aut

oves must be

azardous che

e, if working

ble chemica

f working wi

nstance, a m

ed to provide

nt gloves mu

s) to avoid co

any equipm

hones, comp

tection

mployee mus

nized hazard

hould be cov

rn at all time

rea; shorts a

ctive suits ar

l procedure

s open and t

tted, etc.). L

vidual and b

o their full le


er; regardles

hemical con

ortant to eva

being handle

hers. The SD

chart should

m a manufact

at each lab p

more versatile

do) with a m

When handli

toclaves, ap

e worn.

emical being

g with a sma

al‐resistant g

th a large vo

more durable

e adequate

ust not be w

ontaminatio

ment that cou

uter work st

st wear prote

ds. All unpro

vered. Full le

es by all indi

re not perm

re required t

s using haza

the chemica

Laboratory c

be fastened (

ength. Labo

65

ocument will only

ss of whethe

tainers are c

aluate the ef

ed. Some glo

DS for the sp

be consulte

turer that do

purchase a g

e and provid

minimum of a

ng chemical

propriate pr

g handled sh

ll volume of

gloves provid

olume of sod

e glove such

protection.

worn outside

n of public a

uld likely res

tations). Dis

ective clothi

otected skin

ength pants

viduals that

mitted. Lab co

to be worn w

rdous chem

l is being po

coats must b

(snap button

ratory coat s

y be maintained on

er the contai

contaminate

ffectiveness

oves are mo

pecific chem

ed to select t

oes not prov

eneral purpo

de resistance

a 4 mil thickn

s with harm

rotection suc

hould be con

f a sodium hy

de adequate

dium hydrox

as a butyl ru

of the labor

areas. Glove

sult in cross‐

posable glov

ing to protec

surfaces tha

or full‐lengt

t are occupy

oats, covera

while workin

micals (e.g.,

oured,

be appropria

ns are

sleeves mus

Chapte

nline.

ner is open

ed). When se

of the glove

ore suitable f

ical being ha

the most ap

vide an adeq

ose disposab

e to a wider

ness that is s

mful tempera

ch as cryoge

nsidered as

ydroxide

e

xide as with

ubber

ratory (e.g.,

es should als

‐contaminat

ves must nev

ct the

at are

th

ing

lls,

ng on,

tely

st be of a suf

r 6: Laboratory PP

or closed (it

electing

e type to the

for certain

andled and t

propriate glo

quate glove

ble nitrile gl

range of

suitable for

ature extrem

enic gloves o

hallways,

o be remove

tion (e.g., wa

ver be reuse

fficient lengt

PE Policy

t

e

the

ove.

ove

mes

or

ed

ater

ed.

th to

preve

when

It is r

durin

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trave

in com

respo

coats

facilit

empl

6.4.7

Close

labor

perm

must

wher

acros

expo

the fo

need

6.5 M

Support s

to perfor

present i

with haza

include s

unique sa

support s

6.6 P

Laborato

with haza

ent skin expo

n working wi

ecommende

ng these proc

ratory coats

eling directly

mmon areas

onsible for p

s. They may

ties. Alterna

oyees to rep

Foot Prot

ed toe shoes

ratory; open

mitted in any

wear prote

re there is a

ss the foot, p

sed to electr

oot (e.g., cle

to worn as

Minimum P

staff (e.g., cu

rm routine ta

n the labora

ardous chem

safety glasse

afety require

staff and/or

PPE Trainin

ory Superviso

ardous mate

The officia

osure while

ith pyrophor

ed that 100%

cedures to m

should not

y to an adjac

s such as bre

roviding lau

not be clean

tives to laun

place contam

tection

must be wo

toe shoes a

circumstanc

ctive footwe

high‐risk of

piercing the

rical or chem

eaning up a c

well.

PPE Requir

ustodians, m

asks such as

atory, but ar

micals. To be

s, long pant

ements mus

visitors of th

ng Require

ors must ens

erials occurs


wearing glo

ric materials

% cotton (or

minimize inju

be worn out

ent laborato

eak rooms, o

ndry service

ned by staff

ndering lab c

minated lab

orn at all tim

and/or sanda

ce. Each affe

ear when wo

objects fallin

sole, and wh

mical hazard

chemical spi

rements fo

maintenance

maintenanc

e not perfor

e present in t

s, and closed

st be followe

he additiona

ments

sure that all

. This trainin

66

ocument will only

ves. Flame r

s or flammab

other non‐s

ury in the ca

tside of a lab

ory work are

offices, or re

es as needed

members at

coats include

coats, or usi

mes when in t

als are not

ected emplo

orking in are

ng on or roll

here the fee

s. If there is

ll on the floo

or Support

e workers) an

ce or take a

rming work w

the laborato

d‐toe shoe.

ed, it is the la

al requireme

employees

ng must be d

y be maintained on

resistant lab

ble liquids gr

synthetic ma

ase of a fire e

boratory unl

ea. Laborato

strooms. Ea

d to maintain

t private resi

e routinely p

ing disposab

the

oyee

eas

ing

et are

a high risk o

or), then che

t Staff and

nd visitors o

tour of the

with or direc

ory, the mini

If additional

ab personne

ents.

receive PPE

documented

Chapte

nline.

oratory coat

reater than

aterial) cloth

emergency.

less the indi

ry coats sho

ch departm

n the hygien

idences or p

purchasing n

ble lab coats

of chemical c

emical‐resist

Visitors

ften must e

lab. These in

ctly adjacent

imum PPE re

l PPE is requ

el’s responsi

training bef

d. Document

r 6: Laboratory PP

ts must be w

1 liter in vol

hing be worn

vidual is

uld not be w

ent is

ne of laborat

public laundr

new lab coat

.

contaminati

tant booties

nter laborat

ndividuals ar

t to any wor

equirements

ired or if oth

bility to not

fore any wor

t PPE trainin

PE Policy

worn

ume.

n

worn

tory

ry

ts for

on to

s may

tories

re

rk

s

her

ify

rk

g


67


using Certification of Training form (Appendix B of the Purdue University Personal Protective

Equipment (PPE) Policy: http://www.purdue.edu/rem/home/booklets/PPEPolicy.pdf) or access

it directly from http://www.purdue.edu/rem/home/forms/CertT.pdf. Each lab employee must

be trained to know at least the following:

When PPE is necessary;

What PPE is necessary;

How to properly don, doff, adjust, and wear PPE;

The limitations of the PPE; and

The proper care, maintenance, and useful life of PPE.

Each affected employee must demonstrate an understanding of the training provided, and the

ability to use the PPE properly, before performing any work requiring the use of PPE. When the

supervisor has reason to believe that an affected employee who has already been trained does

not have the understanding and skill required (e.g., employee is seen handling hazardous

materials without wearing proper PPE), then the supervisor must ensure the employee is

retrained.

6.7 Injuries, Illnesses, and Medical Examinations

Employees must notify their Laboratory Supervisor of all injuries and illnesses regardless of how

the magnitude. The laboratory supervisor must ensure that a First Report of Injury form is

completed. Employees should report to a Purdue approved occupational medical provider

(http://www.purdue.edu/hr/Benefits/wcimmediatecare.html#treatmentFacilities) if medical

attention is required (Note: The Purdue University Student Hospital is not an approved

occupational medical provider). If the injury is serious and presents an emergency situation, dial

911 and emergency responders (Purdue Fire Department if located on the West Lafayette

Campus) will respond and transport the patient to a local hospital emergency room. For more

information regarding the First Report of Injury reporting process, visit the REM webpage

(http://www.purdue.edu/rem/injury/froi.htm).

Departments must provide all employees who work with hazardous chemicals an opportunity

to receive medical attention, including any follow‐up examinations which the examining

physician determines to be necessary, under the following circumstances:

Whenever an employee develops signs or symptoms associated with a hazardous

chemical to which the employee may have been exposed in the laboratory;

Where exposure monitoring reveals an exposure level routinely above the action level

(or in the absence of an action level, the permissible exposure limit) for an OSHA


68


regulated substance for which there are exposure monitoring and medical surveillance

requirements, medical surveillance shall be established for the affected employee as

prescribed by the particular standard; and

Whenever an event takes place in the work area such as a spill, leak, explosion, or other

occurrence resulting in the likelihood of a hazardous exposure, the affected employee

shall be provided an opportunity for a medical examination. All medical examinations

must be performed by or under the direct supervision of a licensed medical care

provider and must be provided without cost to the employee.

Chapt

7.1 In

Hazardou

human h

the Envir

Managem

program

or discar

regulatio

of this ch

Hazardou

mixtures

fluids or

one of th

C

lis

Li

(F

ch

U

lit

e

b

W

M

EPA‐regu

more det

Appendic

(http://w

Hter 7:

ntroductio

us waste is g

health or the

ronmental P

ment. The la

in a safe an

ded in the tr

ons or the W

hapter for in

us wastes ca

generated f

pesticides),

he following

haracteristic

sted).

isted Wastes

F‐listed), was

hemical prod

Universal Wa

thium, nicke

quipment (e

ulbs). Note:

Wastes and c

Mixed Waste

ulated hazar

tailed definit

ces 1 and 2 o

www.purdue

The officia

Hazardou

on

generally def

environmen

rotection Ag

aboratory su

d compliant

rash unless i

West Lafayett

formation a

an be liquid,

from researc

or by‐produ

categories:

c Wastes: in

s: includes w

stes from sp

ducts (U‐ an

aste: include

el‐cadmium,

e.g., thermom

alkaline bat

can be legally

e: hazardous

dous waste

tion of haza

of the Hazar

e.edu/rem/h


us Waste

fined as was

nt. Hazardou

gency (EPA)

pervisor is r

t manner. No

it is certain t

te wastewate

nd guidance

solid, gas, o

ch and teach

ucts of manu

cludes waste

wastes from

pecific indust

d P‐listed).

s certain bat

nickel meta

meters, ther

tteries and in

y disposed o

waste mixe

should not b

rdous waste

rdous Waste

home/bookle

69

ocument will only

e Manag

ste that is da

us waste reg

and the Indi

esponsible f

o chemical w

that doing so

er treatmen

e for accepta

or sludge. Th

hing operatio

ufacturing pr

es that are ig

common ma

tries (K‐liste

tteries (prim

al hydride, an

rmostats), an

ncandescent

of as trash.

d with radio

be mistaken

e, including t

e Disposal Gu

ets/hwdg.pd

y be maintained on

gement

angerous or

gulations are

iana Departm

for managing

waste should

o does not v

nt plant’s req

able sink disp

ey can be di

ons, comme

rocesses. All

gnitable, co

anufacturing

d), and wast

marily rechar

nd mercury

nd certain la

t bulbs are n

oactive wast

for biologic

the D, F, P, a

uidelines.

df)

Chapter 7: Hazard

nline.

potentially h

e strictly enf

ment of Env

g the hazard

d be poured

violate hazar

quirements (

posal practic

iscarded che

ercial produc

hazardous w

rrosive, reac

g and indust

tes from com

rgeable batte

oxide), merc

amps (e.g., f

not consider

e.

cal or radiolo

and U lists, is

dous Waste Mana

harmful to

forced by bo

vironmental

dous waste

down the d

rdous waste

(see section

ces).

emicals or

cts (e.g., clea

waste falls in

ctive, or toxi

trial process

mmercial

eries such

cury‐contain

fluorescent

red Universa

ogical wastes

s provided in

agement

oth

rain

7.7

aning

nto

ic (D‐

es

ning

l

s. A

n

7.2 W

All chem

laborato

properly

a waste s

laborato

chemical

characte

Section 1

characte

All waste

Containe

waste an

label pro

percenta

as “trace

laborato

as “Haza

Waste Iden

ical constitu

ry personne

characterize

stream resul

ry superviso

l products us

rization. Add

13, “Disposa

ristics.

e should be p

ers must be l

nd the words

ovided by RE

ages is not as

e”) as listing

ry and the co

rdous Waste

The officia

ntification

uents in a ha

l. Not only is

ed and dispo

lting from an

or for assista

sed in the ex

ditionally, re

l Considerat

properly lab

abeled and

s "Hazardous

M in a perce

s important

all of the ch

omposition

e – awaiting

Figure 7.1 –


and Label

zardous was

s this require

osed of by R

n experimen

nce. In most

xperimental

eview SDSs (

tions”) to ob

eled as soon

clearly mark

s Waste". Ha

entage form

(+ 5% is acce

emicals that

is unknown,

proper char

– Purdue Uni

70

ocument will only

ing

ste containe

ed by the EP

EM. If there

ntal process,

t cases, care

protocol wi

specifically S

btain informa

n the first dr

ked with wo

azardous wa

at as shown

eptable and

t makeup th

, it should be

racterization

iversity Haza

y be maintained on

er must be id

PA, it also en

is uncertain

laboratory

eful documen

ll result in a

Section 2, “H

ation about

op of waste

rds that des

aste should b

n in Figure 7.

constituent

e waste. If a

e assumed t

n”.

ardous Was

Chapter 7: Hazard

nline.

dentified by

nsures that w

nty about th

employees m

ntation and

ccurate was

Hazard Ident

hazardous c

enters a wa

scribe the co

be listed com

1. Listing ac

ts less than 1

a chemical is

to be hazard

ste Label

dous Waste Mana

knowledgea

waste can be

e compositio

must consul

review of al

ste stream

tification” an

constituents

aste containe

ontents of th

mpletely on

curate

1% can be lis

s found in th

ous and labe

agement

able

e

on of

t the

l

nd

and

er.

he

the

sted

e

eled

Chapter 7: Hazardous Waste Management

71


7.3 Waste Storage Requirements

Hazardous waste containers in Purdue laboratories are stored in satellite accumulation area

(SAA). SAAs are used to manage hazardous waste in laboratories and shops because doing so

provides safe and effective means to accumulate hazardous waste before removal by REM.

Additionally, SAAs provide the least restrictive regulatory option for the accumulation and

storage of hazardous waste containers. The following SAA rules must be followed at all times

when managing hazardous waste in a laboratory:

All waste must be stored in containers.

Containers must be in good condition and

compatible with the waste they contain (no corrosive

waste in metal containers).

Containers must be kept closed at all times except

when adding or removing waste.

Containers must be labeled or clearly marked with

words that describe the contents of the waste (e.g., liquid chromatography waste) and

the words "Hazardous Waste".

Containers must be stored at or near the point of generation and under the control of

the generator of the waste (wastes should remain in the same room they were

generated in). A central waste collection room should not be established.

The waste storage volume should never exceed 55 gallons per SAA.

Containers should be segregated by chemical compatibility during storage (e.g., acids

away from bases, secondary containment can be used as a means of segregation).

Avoid halogenated and non‐halogenated wastes in the same waste container.

Avoid mixing incompatible waste streams in the same container (e.g., acids with bases,

oxidizers with organic solvents) that will potentially create an exothermic reaction in the

waste container. If mixing waste streams does create heat, allow the container to vent

and cool in a chemical fume hood before sealing to avoid over pressurization of the

container as illustrated in Figure 7.2.

Collect all highly toxic, reactive, mercury and any exotic wastes (e.g., dioxin compounds,

PCBs, controlled substances) separately even if they are chemically compatible with

other waste streams. Failing to do so can result in costly disposal fees (e.g., mixing

mercury with an organic solvent waste means that the entire waste stream must be

treated as mercury waste).

All spills and leaks should be cleaned up immediately.

Identification of SAAs is not required by the EPA, but it is recommended as a good

practice.

Hazardous Waste

Satellite

Accumulation Area

CAUTION

7.4 W

REM doe

waste to

can be us

waste co

If reques

returned

room nu

disposed

Figure 7.2

Waste Cont

es not provid

provide con

sed (e.g., 4‐l

ontainers suc

ted, reusabl

d to the gene

mber as illus

d of and not

The officia

2 – Containe

tainers

de container

ntainers. Usu

iter glass jar

ch as 20‐liter

le hazardous

erator's area

strated in Fig

returned.


r Failure Du

rs to campus

ually the orig

r, 5‐gallon gr

r carboys as

s waste stora

a. Mark the c

gure 7.4. Co

72

ocument will only

e to Mixing

s. It is the res

ginal contain

reen metal s

shown in Fig

age containe

container cle

ntainers uns

y be maintained on

Incompatib

sponsibility

ner of the m

solvent can)

gure 7.3 for

ers of 5 gallo

early with "R

suitable for

Chapter 7: Hazard

nline.

ble Waste St

of the gener

main compon

. Purdue Sto

sale.

ons or larger

Return to", t

reuse will be

dous Waste Mana

treams

rator of the

nent of the w

ores also offe

r may be

he building,

e properly

agement

waste

ers

and

Purdue’s

and the P

that som

dumpste

pressuriz

keep the

T

C

Id

co

o

(7

la

R

to

A

B

co

d

If

m

M

Figure 7.

s policy for th

Physical Faci

me chemical r

er or compac

zed during co

following p

riple rinse e

ollect the rin

dentify triple

ontainers by

n the contai

765) 49‐4012

abels.

emove any c

o become pr

Arrange remo

uilding Serv

ontainers to

umpster out

f unable to re

materials from

Materials Pic

The officia

.3 – 20 Liter

he disposal o

ilities Buildin

residues hav

ctor causing

ompaction, w

rocedures a

mpty contai

nsate for dis

e‐rinsed, dry

y placing a “S

iner (Figure

21 to reques

cap that ma

ressurized w

oval of these

ices staff in y

o the designa

tside your b

emove resid

m container

kup Request


Carboy

of empty co

ngs and Grou

ve the poten

a reaction o

which may r

nd informat

ners with a

sposal throug

y, odorless, a

Safe for Disp

7.5). Contac

st a supply o

y cause the

when compac

e containers

your area or

ated area be

uilding.

dual hazardo

rs, submit th

t Form.

73

ocument will only

Figure

ntainers is im

unds staff w

ntial to mix w

or fire. In add

result in resi

tion in mind

solvent capa

gh REM.

and empty

posal” label

ct REM at

of these

container

cting.

with the

r take these

eside the

ous

ese to REM

y be maintained on

e 7.4 – Reus

mplemented

when removi

with other in

dition, seale

dues sprayin

when dispo

able of remo

for pickup u

Figure 7

Chapter 7: Hazard

nline.

sable Waste

d to protect

ng trash. Ple

ncompatible

d containers

ng onto wor

osing of emp

oving the ori

using the Ha

.5 – Safe for

dous Waste Mana

e Container

Purdue faci

ease remem

residues in

s may becom

rkers. Please

ty container

iginal mater

zardous

r Disposal La

agement

lities

ber

the

me

e

rs:

ial.

abel

7.5 W

REM pro

A Hazard

generato

REM staf

The follo

campus l

1. P

t

2. A

3. A

4. C

R

For furth

the REM

Waste Disp

vides pickup

dous Materia

or of the was

ff will come t

wing proced

ocations:

Prior to pick

the waste w

All waste mu

All container

Complete an

REM webpag

er informati

webpage (h

The officia

posal Proce

p services fo

als Pickup Re

ste to initiate

to your lab t

dures must b

up, all wast

as generate

ust be placed

rs must be c

nd submit a

ge to find th

ion regardin

http://www.

Figure 7.6


edures

r all chemica

equest Form

e pickup ser

to pick up th

be followed

te must be p

d.

d in an appr

apped and l

Hazardous M

he online Haz

ng hazardous

purdue.edu

6 – Hazardo

74

ocument will only

al waste gen

m must be co

rvices. Once

he waste. Av

in order to h

placed in a de

opriate cont

abeled.

Materials Pic

zardous Mat

s waste disp

/rem/hmm/

ous Material

y be maintained on

nerated on th

ompleted an

the pickup r

verage turna

have hazard

esignated ar

tainer(s).

ckup Reques

terial Pickup

osal, call RE

/hmm.htm).

s Pickup Req

Chapter 7: Hazard

nline.

he West Lafa

d submitted

request has

round time

ous waste re

rea within th

st Form (Figu

p Request su

M at (765) 4

quest

dous Waste Mana

ayette camp

d by the

been proces

is 3‐5 days.

emoved from

he room whe

ure 7.6). Visi

ubmission fo

49‐40121 or

agement

pus.

ssed,

m

ere

it the

orm.

visit

7.6 U

Unknown

stored in

be taken

properly

noted inc

subsectio

7.6.1

Until

conta

inform

chara

7.6.2

Every

are a

Unknown C

n chemicals

n labs for yea

to assist wit

identified a

clude: corro

ons describe

Labeling U

the unknow

ainer should

mation shou

acterization

Identifyin

y effort shou

few steps th

The officia

Chemical W

are a seriou

ars before la

th proper m

ccording to

sive, flamma

e in detail ho

Unknown Ch

wn chemical

be labeled w

uld be writte

by REM” as

Figure 7

ng Unknown

uld be made

hat can be ta


Waste

s problem in

b personnel

anagement

hazard class

able, oxidize

ow to proper

hemicals

can be prop

with a Hazar

en on the lab

illustrated in

7.7 – Properl

n Chemicals

by laborato

aken to help

75

ocument will only

n laboratorie

l notice the u

of unknown

s before prop

er, reactive, t

rly manage u

perly identifi

rdous Waste

bel: “Unknow

n Figure 7.7.

ly Labeled U

ory personne

p this effort:

y be maintained on

es. Mysterio

unidentified

ns. Unknown

per disposal

toxic, and ra

unknown ch

ed by either

e Disposal Ta

wn hazardou

.

Unknown W

el to identify

Chapter 7: Hazard

nline.

ous chemical

d items. How

n chemicals

l. The hazard

adioactive. T

emicals.

r lab staff or

ag. The follo

us chemical,

aste

y unknown c

dous Waste Mana

ls are often

wever, steps

must be

ds that shou

The following

REM, the

owing

awaiting pr

hemicals. He

agement

can

ld be

g

roper

ere


76


1. Ask other laboratory personnel if they are responsible for, or can help identify the

unknown chemical.

2. The type of research conducted in the laboratory can be useful information for

making this determination. Eliminating certain chemicals as a possibility helps

narrow the problem as well. This is especially important for mercury, PCB, or dioxin

compounds because they must be managed separately from other hazardous waste.

3. For trade products, contact the manufacturer or search online to obtain an SDS.

REM staff can assist you in finding an SDS.

7.6.3 Removing Unknown Chemicals from the Work Area

If it is not possible to identify the material, a "Hazardous Waste" label should be placed on

the container as described above in Section 7.6.1 and a Hazardous Materials Pickup Request

Form should be submitted which describes all of the available information (e.g., 4‐liter

container of clear liquid). Call REM at (765) 49‐40121 if you have a question about an

unknown.

7.6.4 Preventing Unknown Chemicals

Here are a few tips that will help prevent the generation of unknown chemicals:

Label all chemical containers, including beakers, flasks, vials, and test tubes. The

label should be placed on the container, not the cap to avoid accidental mislabeling.

Immediately replace labels that have fallen off or that are deteriorated.

Label containers using chemical names. Do not use abbreviations, structure, or

formulae.

Archived research samples are often stored in boxes containing hundreds of small

vials. Label the outside of the box with the chemical constituents paying special

attention to regulated materials such as radioactive material, organic solvents,

heavy metals and other toxics. If the samples are nonhazardous, label them as such.

Submit frequent Hazardous Materials Pickup Request Forms to reduce the amount

of chemicals in your laboratory.

Employees should dispose of all of their waste before leaving/graduating from

Purdue. The lab and/or department should come up with a system to ensure that all

faculty, staff, and students properly dispose of hazardous waste, including unwanted

research samples, before employees leave.


77


7.7 Sink and Trash Disposal

No chemical waste should be poured down the drain or discarded in the trash unless it is

certain that doing so does not violate hazardous waste regulations or the West Lafayette

wastewater treatment plant’s discharge requirements. In order to ensure improper disposal

does not occur, the detailed instruction and guidelines for acceptable sink disposal is provided

in the Purdue University Hazardous Waste Disposal Guidelines (Chapter 5.6, Appendix E, and

Appendix F: http://www.purdue.edu/rem/home/booklets/hwdg.pdf). Please contact REM at

(765) 49‐40121 for further information regarding non‐hazardous chemical waste disposal.

7.8 Sharps Waste

Sharps are items capable of puncturing, cutting, or abrading the skin such as glass or plastic

pipettes, broken glass, test tubes, petri dishes, razor blades, needles, and syringes with needles.

Sharps waste contaminated with hazardous chemicals must be placed into puncture resistant

containers (e.g., sharps container, plastic or metal container with lid) and properly labeled as

detailed in Chapter 4 of the Purdue University Hazardous Waste Guidelines. All chemically

contaminated waste should be inventoried on a Hazardous Materials Pickup Request Form and

sent to REM for proper disposal.

Clean uncontaminated broken glassware and plastic sharps should be placed in a corrugated

cardboard box or other strong disposable container. Do not exceed 20 pounds. When ready for

disposal, the box should be taped shut and prominently labeled as “Sharp Objects/Glass –

Discard” or similar wording. The “Safe for Disposal” label (Figure 7.5) should also be affixed to

the outside of the container. Contact your Physical Facilities Building Services department for

specific non‐hazardous waste disposal instructions. More detail regarding sharps, including

biologically contaminated sharps, can be found at the found in the REM Sharps and Infectious

Waste Handling and Disposal Guidelines.

(http://www.purdue.edu/rem/home/booklets/sharps.pdf)

7.9 Liquid Chromatography Waste

Liquid chromatography (LC) is an analytical technique used to separate, identify, quantify, and

purify individual components of a mixture. This technique is very common in biological and

chemical research. The most common type of LC at Purdue is High Performance Liquid

Chromatography (HPLC). Purdue has numerous LC instruments located in laboratories all over

campus. Because organic solvents (e.g., methanol, acetonitrile) are commonly used in the

process, most LC waste is regulated by the EPA as hazardous waste. Consequently, all

containers collecting LC waste must remain closed while the LC unit is in operation. It is neither

acceptab

acceptab

One of th

regulatio

1. P

7

ca

2. A

a

si

co

a

a

sh

fo

ble to place a

ble to use foi

Op

he following

ons for LC wa

urchase an e

.9 shows sev

an be purcha

Safety C

An existing ca

n existing ca

imilar to the

ontainer ope

ny exhaust f

4‐liter conta

hould be rep

or REM picku

The officia

a waste line

il or Parafilm

pen Waste

Figure 7.8

practices m

aste collectio

engineered c

veral examp

ased.

Cans Po

Figure 7.9 –

ap can be mo

ap, a hole ca

e diameter o

ening and w

filter or air v

ainer or 5‐ga

placed with a

up. See Figu


running from

m® as a mean

– Improper

must be emp

on systems:

container an

ples of accep

orted Cap (No

– Proper LC W

odified by th

n be drilled

f the waste

waste line. In

valve tube th

allon carboy

a regular, un

re 7.10 for e

78

ocument will only

m the LC un

ns of closure

LC Waste C

loyed in ord

nd/or cap de

table solutio

Threads) 4 P

Waste Colle

he research

into a cap. T

line; there s

addition, a

hat may be re

be used for

nmodified ca

examples of

y be maintained on

it into an op

e as shown i

Foil

ollection Pr

er to comply

esigned for L

ons for prop

ort (threaded

ection Optio

lab for LC w

The diamete

should be a t

hole should

equired. It is

r waste colle

ap once the

acceptable

Chapter 7: Hazard

nline.

pen waste co

n Figure 7.8

actices

y with hazar

LC waste col

per LC waste

d) Cap Sol

ns for Purch

waste collecti

er of the hole

tight fit betw

be drilled to

s recommen

ection. The m

container is

modified ca

dous Waste Mana

ontainer nor

.

Parafilm®

rdous waste

lection. Figu

collection t

lvent Bottle C

hase

ion. To mod

e should be

ween the

o accommod

nded that eit

modified cap

s full and rea

ps.

agement

r is it

ure

hat

Cap

ify

date

ther

p

ady

Chapt

Chemica

environm

order to

emergen

8.1 N

Non‐eme

toxic or r

in a publ

personne

spill kit. I

should be

material

such as o

appropri

all times

(specifica

informat

8.2 E

Emergen

greater t

compoun

environm

(e.g., res

cleanup.

followed

spill:

C

p

If

ta

Cter 8:

l spills in the

ment. All lab

minimize ris

ncy chemical

Non‐Emerg

ergency chem

reactive mat

ic area such

el using conv

n general, w

e isolated, e

should be a

oil dry or kitt

ate solvent (

and only tra

ally Section 6

ion.

mergency

ncy chemical

han 1 liter, i

nd, present a

mental hazar

pirator) and

The followin

in the even

ease all activ

ersonnel in t

f chemical ex

aken to the n

The officia

hemical

e laboratory

personnel m

sk. In genera

spills, or em

gency Chem

mical spills a

terial, do not

as a hallway

ventional lab

when a non‐e

everyone in t

bsorbed and

ty litter. Dec

(soap and w

ained person

6, “Accident

y Chemical

spills are ge

involve a hig

an immediat

rd, or require

specialized

ng procedur

t of an emer

vities and im

the area are

xposure has

nearest safe


l Spills

can pose a s

must be train

al, chemical s

mergency ch

mical Spill

are generally

t present a s

y. These spil

b PPE (e.g., s

emergency s

the lab shou

d collected u

ontaminatio

water is often

nnel should c

tal Release M

Spill Proce

enerally defi

ghly toxic or

te fire or

e additional

training to p

res should be

rgency chem

mmediately e

e aware of th

occurred to

ety shower a

79

ocument will only

significant ri

ned on how

spills can be

emical spills

Procedure

y defined as

significant fir

ls can be cle

safety glasse

spill occurs i

ld be made

using either

on of the spi

n the most e

conduct the

Measures”) t

edures

ned as

reactive

PPE

properly

e

mical

evacuate the

he spill and a

o the skin or

nd eyewash

y be maintained on

isk to human

to properly

e placed into

s.

es

less than 1

re or environ

eaned up by

es/goggles, l

n the lab the

aware of th

pads or som

ll area shou

effective). Pr

e cleanup. Ad

to obtain che

e affected ar

also evacuat

eyes, the af

h station.

C

nline.

n health and

respond to

o one of two

liter, do not

nmental haz

properly tra

ab coat, glov

e area aroun

e spill, and t

me other abs

ld be conduc

roper PPE sh

dditionally, r

emical‐speci

rea (make su

te).

ffected perso

Chapter 8: Chemic

d the

chemical sp

categories:

involve a hi

zard, and are

ained lab

ves) and the

nd the spill

the spilled

orbent mate

cted using a

ould be wor

review the S

ific cleanup

ure that all

onnel should

cal Spills

pills in

non‐

ghly

e not

e lab

erial

n

rn at

SDS(s)

d be

D

re

o

T

fo

8.3 C

Each labo

purchase

handle sm

with resp

A

ki

N

a

st

C

co

ge

P

co

C

th

Dial 911, whic

esponse, if t

ccurred, a fi

he fire alarm

ollowing occ

o A fire

explos

o The la

enviro

o Toxic

from t

o You a

occup

o Ensur

prope

Chemical S

oratory shou

ed from a ve

mall spills of

ponse and cl

Absorbent m

itty litter, bo

Neutralizing a

nd/or bases

tored in the

ontainers su

ontainerize s

enerated du

PE such as g

oat or apron

aution tape

he spill

The officia

ch will initia

he situation

re or explos

m should be

curs:

and/or expl

sion);

arge spill (wh

onmental ha

vapors are l

the laborato

re unsure of

pants.

e that no on

erly cleaned

pill Kits

uld have a sp

ndor or crea

f the most co

eanup mate

aterials such

ooms, or pill

agents (e.g.,

if high volu

laboratory

uch as drums

spilled mate

uring the clea

gloves, safety

n, chemical‐r

or some oth


te both the

is, or could

ion has occu

pulled, whic

osion has oc

hich is either

azard) is in a

eaving the a

ory into the h

f the hazards

ne else is allo

up by the Pu

pill response

ated by lab p

ommon haza

erials such as

h as pads, bo

ows

Neutrasorb

me of acids

s, buckets, a

erial and con

anup proces

y glasses and

resistant boo

her means to

80

ocument will only

Purdue Polic

become an

urred).

ch will initiat

ccurred (or t

r highly toxic

public area

area where t

hallway or n

s and feel th

owed to ente

urdue Fire D

e kit availabl

personnel, b

ards in the la

s:

ooms, oil dry

b®) for acids

and/or base

and/or bags

ntaminate de

ss

d/or goggles

oties

o warn peop

y be maintained on

ce and Purd

emergency

te building e

there is a thr

c or present

such as a ha

the spill has

neighboring r

hat the spill c

er the area u

Department.

e for use. La

but each spill

aboratory. T

y or

es are

to

ebris

s, lab

ple of

C

nline.

ue Fire Depa

(e.g., chemi

evacuation, i

reat of fire a

s an immed

allway;

occurred, su

rooms;

could be har

until the spil

ab spill kits c

l kit should b

The kit shoul

Chapter 8: Chemic

artment

ical exposure

f any of the

and/or

iate fire or

uch as seepi

rmful to buil

ll has been

can either be

be equipped

ld be equipp

cal Spills

e has

ng

lding

e

d to

ped

Chapt

Effective

must act

trained b

training r

type of re

requirem

49‐6371

(http://w

9.1 C

As discus

technicia

training b

responsib

R

R

R

R

Refer to

CHP train

9.1.1

After

CHP r

the in

Tter 9:

training is c

ively particip

before any w

requirement

esearch bein

ments that ar

or visit the R

www.purdue

CHP Trainin

ssed in Chap

ans/manage

before any w

ble for provi

eview the la

eview lab‐sp

eview lab‐sp

eview any o

Appendix A

ning.

Annual CH

r receiving th

refresher tra

nitial CHP tra

Review of

Review of

Review of

Review of

The officia

raining

crucial to a s

pate in the t

work with ha

ts for all Pur

ng conducte

re not detail

REM Trainin

e.edu/rem/h

ng

pter 1 of the

rs, post‐docs

work with ha

iding CHP tra

ab‐specific C

pecific hazar

pecific SOPs

other lab‐spe

for the CHP

HP Refreshe

he initial doc

aining as we

aining, but s

f the lab‐spe

f the lab‐spe

f any additio

f chemical sp


uccessful lab

training proc

zardous mat

due laborato

d and assoc

ed in this ch

g webpage.

home/files/tr

CHP, all labo

s, visiting sci

azardous ma

aining. Initia

HP in its ent

rd assessmen

ecific protoco

Training Ce

er Requirem

cumented CH

ll. This annu

hould includ

ecific hazard

ecific SOPs

onal lab‐spec

pill and lab e

81

ocument will only

boratory saf

cess to ensur

terials occur

ories. It shou

iated hazard

hapter. For m

raining.htm)

oratory emp

ientists, etc.

aterials occu

al CHP trainin

tirety

nts

ol or require

rtification Fo

ments

HP training,

al refresher

de at least th

assessment

cific rules an

emergency p

y be maintained on

fety program

re that all la

rs. This chap

uld be noted

ds, there ma

more inform

)

ployees (PIs,

.) must recei

rs. The Labo

ng should in

ements

orm, which c

all lab empl

r training can

he following

ts (review of

nd requireme

procedures

nline.

m. Laborator

b employee

pter details t

d that depen

ay be additio

ation, conta

graduate st

ive documen

oratory Supe

clude the fo

can be used

oyees must

n be a conde

g elements:

f PPE require

ents

Chapter 9: T

ry Supervisor

es are effecti

he minimum

nding on the

onal training

act REM at (7

tudents, lab

nted CHP

ervisor is

ollowing:

to documen

receive ann

ensed versio

ements)

Training

rs

vely

m

e

765)

nt

nual

on of

Chapter 9: Training

82


9.2 PPE Training

As discussed in Chapter 6 of the CHP, Laboratory Supervisors must ensure that all lab

employees receive documented PPE training before any work with hazardous materials occurs.

Document PPE training using the Certification of Training form (Appendix B of the Purdue

University Personal Protective Equipment (PPE) Policy:

http://www.purdue.edu/rem/home/booklets/PPEPolicy.pdf) or get it directly from

http://www.purdue.edu/rem/home/forms/CertT.pdf. Each lab employee must be trained to

know at least the following:

When PPE is necessary

What PPE is necessary

How to properly don, doff, adjust, and wear PPE

The limitations of the PPE

The proper care, maintenance, and useful life of PPE

Each affected employee must demonstrate an understanding of the training provided, and the

ability to use the PPE properly, before performing any work requiring the use of PPE.

9.3 SOP Training

As discussed in Chapter 4 of the CHP, SOPs are written instructions that detail the steps that

will be performed during a given procedure and include information about potential hazards

and how these hazards will be mitigated. SOPs must be developed for all high‐hazard tasks that

are performed in the lab, which is defined as work with:

Explosives

Water‐reactive, pyrophoric, self‐heating, or self‐reactive chemicals

Particularly hazardous substances, which includes carcinogens, reproductive toxins, and

acutely toxic substances

Compressed gases

Work involving more than 1 liter of flammable liquids, flammable solids, corrosives,

oxidizers, or organic peroxides at one time

High‐hazard tasks can also include work with equipment that creates particularly

hazardous conditions. Examples include solvent distillation, work with high‐pressure

systems, hydrogenation, work with cryogenic chemicals such as liquid nitrogen, etc.

Chapter 9: Training

83


Laboratory Supervisors must ensure that all applicable personnel receive documented training

on lab‐specific SOPs. More information regarding SOPs can be found on the REM website.

(http://www.purdue.edu/rem/home/files/sop.htm)

9.4 Laboratory Chemical Safety Course (CHM 605)

The Purdue Department of Chemistry offers a graduate level, zero credit fall‐semester course

(CHM 605) that covers the fundamentals of laboratory chemical safety practices. CHM 605

includes a strong focus on learning how to find, read, interpret and use the information in

safety data sheets, chemical labels, and other printed chemical safety information. Other topics

covered include fire protection/prevention, electrical safety, laser and ionizing radiation safety,

machine safety (pumps, autoclaves, centrifuges), and non‐PPE safety equipment (hoods,

extinguishers, fire protection systems and building elements, general ventilation, showers,

eyewashes), and an understanding of administrative controls, engineering controls, and how to

select, use, maintain and decide to retire/replace PPE necessary for laboratory work with

chemicals. Regulatory agency familiarity and compliance topics including OSHA, EPA, DOT, and

NRC are also included. CHM 605 is open to all departments and is a required course for some

departments. All graduate students working in a laboratory environment are strongly

encouraged to take CHM 605.

9.5 REM Researcher’s Guide

The CHP focuses on work with hazardous chemicals in the laboratory. However, other common

types of hazards are present in many research labs as well (e.g., biological hazards, lasers, etc.).

REM has developed the Researcher’s Guide as a tool to assist researchers with compliance and

training requirements for a broad range of common hazards and regulatory programs found in

the laboratory. Visit the REM Researcher’s Guide for more detailed information.

(http://www.purdue.edu/rem/home/files/researchers.htm)

Appendix A: CHP Training Certification Form

84


Appendix A:

CHP Training Certification Form

Appendix A: CHP Training Certification Form

85


PURDUE UNIVERSITY

Chemical Hygiene Plan and Hazardous Materials Safety Manual

Awareness Certification (Please Type or Print Legibly)

For: AAIC; Patricia Bishop; BRWN 3154, 5106, 5185

Principal Investigator, Building, and Room(s)

After reading the "Purdue Chemical Hygiene Plan”, complete and return a copy of this form to

WTHR 173 Chemistry raining Records. Your supervisor may want copies kept in the group as

well. By signing below you acknowledge that you are aware of the Chemical Hygiene Plan and

the policies and procedures applicable to the OSHA Occupational Exposure to Hazardous

Chemicals in Laboratories Standard (29 CFR 1910.1450). Your supervisor will provide additional

information and training as appropriate.

Name: Patricia Bishop Work Telephone: 494‐7188

Email Address: [email protected]

Department: Chemistry

Job Title: Laboratory Manager

Employee Signature: Date: 9/12/2014

Filing:

Completed Chemical Hygiene Plan Awareness Certifications are to be filed in a central

administrative location within each staff member’s department. These and all other safety

training records should be organized in a way that allows original records to be retrieved

quickly and efficiently on request by an OSHA inspector or a REM staff member, and to be

retrieved for a single staff member or for an entire work group (identified by PI/supervisor).

Appendix B: OSHA Hazard Class Definitions

86


Appendix B:

OSHA Hazard Class Definitions

B.1 P

FB.1.1

Flammab

vapor to

ethyl ace

vaporizat

hazards o

F

F

a

liq

B

p

th

A

at

a

Lo

(p

p

(a

U

(p

p

Some org

sensitive

forming c

Chapter

FB.1.2

A flamma

through f

Physical

lammable

ble hazards a

cause a fire

etate, xylene

tion. The fol

of flammabl

lammable li

lash point is

pplication of

quid to ignit

oiling point

ressure of a

he liquid cha

Auto ignition

t which self‐

bsence of an

ower explos

percentage)

roducing a f

arc, flame, h

Upper explos

percentage)

roducing a f

ganic solven

organic per

chemicals.

5.7.2 of the

lammable

able solid is

friction. Rea

The officia

Hazards

e Liquids

are material

in the prese

e) are more h

lowing defin

e liquids:

quid is a liqu

s the minimu

f an ignition

te under spe

is the temp

liquid equa

anges into a

n temperatu

‐sustained co

n ignition so

sive limit (LE

of a gas or a

flash of fire i

eat).

sive limit (U

of a gas or a

flash of fire i

ts (e.g., diet

roxides. See

CHP details

e Solids

a solid whic

adily combus


s

s which und

ence of an ig

hazardous at

nitions are im

uid having a

um tempera

source caus

ecified test c

erature at w

ls the atmos

vapor.

re is the min

ombustion w

urce.

EL) is the low

a vapor in ai

n presence o

EL) is the hig

a vapor in ai

n presence o

thyl ether) h

Appendix 3

flammable l

h is readily c

stible solids

87

ocument will only

er standard

gnition sourc

t elevated te

mportant to

flash point

ture at whic

ses the vapo

onditions.

which the va

spheric press

nimum temp

will occur in

west concent

r capable of

of an ignitio

ghest concen

r capable of

of an ignitio

ave the pote

for addition

liquids stora

combustible

are powdere

y be maintained on

conditions c

ce. Flammab

emperatures

understand

no greater t

ch the

ors of a

por

sure and

perature

the

tration

f

n source

ntration

f

n source (ar

ential to form

nal informati

age requirem

e, or may cau

ed, granular

Appendix B: OSHA

nline.

can generat

ble liquids (e

s due to mo

d when evalu

than 93 °C (2

rc, flame, he

m potentiall

ion regardin

ments.

use or contri

r, or pasty su

A Hazard Class Def

e sufficient

e.g., hexane,

re rapid

uating the

200 °F).

at).

ly shock‐

g peroxide

ibute to a fir

ubstances w

finitions

re

hich

are dang

solids are

powders

GB.1.3

Gases un

pressure

under pr

C

e

te

Li

te

R

o

D

so

All comp

which ca

of the ch

flammab

Chapter

PB.1.4

Pyropho

liquid or

ignition s

Self‐heat

substanc

liable to

in that it

long peri

erous if they

e more haza

).

Gases unde

nder pressur

not less tha

essure inclu

ompressed

ntirely gaseo

emperature

iquefied gas

emperatures

Refrigerated

f its low tem

Dissolved gas

olvent.

ressed gases

n explode a

emicals they

ble (propane

5.7.3 of the

Pyrophoric

ric material

solid that ev

source can ig

ting materia

ce, which, by

self‐heat. Th

will ignite o

ods of time

The officia

y can be eas

rdous when

er Pressure

e are gases w

an 280 kPA a

de the follow

gas is a gas

ous at ‐50 °C

≤ ‐50 °C.

s is a gas wh

s above ‐50 °

liquefied ga

mperature.

s is a gas wh

s are hazard

nd act as a p

y contain su

), and corros

CHP details

, Self‐Heat

(also called

ven in small

gnite after co

al is a solid o

y reaction wi

his endpoint

nly when in

(hours or da


sily ignited b

n widely disp

e

which are co

at 20 °C or as

wing:

which when

C; including a

ich when pa

°C.

as is a gas wh

ich when pa

dous due to t

projectile if r

ch as asphyx

sive (hydrog

compressed

ting, and S

“spontaneo

quantities a

oming in con

or liquid, oth

ith air and w

differs from

large amou

ays).

88

ocument will only

y brief conta

persed in a co

ontained in a

s a refrigerat

n packaged u

all gases wit

ackaged und

hich when p

ackaged und

the fact they

ruptured. Co

xiation (carb

gen chloride)

d gases stora

Self‐Reactiv

ously combu

and without

ntact with th

er than a py

without ener

m a pyrophor

nts (kilogram

y be maintained on

act with an i

onfined spac

a receptacle

ted liquid. G

under pressu

h a critical

er pressure

packaged is m

der pressure

y are stored

ompressed g

bon dioxide)

).

age requirem

ve Materia

stible”) is a

an external

he air.

yrophoric

rgy supply, is

ric substanc

ms) and afte

Appendix B: OSHA

nline.

ignition sour

ce (e.g., fine

e at a

Gases

ure is

is partially li

made partial

is dissolved

in compress

gases also ca

, toxicity (nit

ments.

als

s

e

er

A Hazard Class Def

rce. Flamma

ely divided m

iquid at

lly liquid bec

in a liquid p

sed cylinder

arry the haza

tric oxide),

finitions

ble

metal

cause

phase

rs,

ards

Self‐reac

exotherm

Chapter

WB.1.5

A water‐

to produ

metals (e

are comm

Chapter

OB.1.6

An oxidiz

may gene

Hydroge

found in

examples

Chapter

OB.1.7

An organ

may be c

atoms ha

formulat

B

B

B

R

Chapter

ctive materia

mic thermal

5.7.4 of the

Water‐Reac

reactive mat

ce a flamma

e.g., sodium,

mon water‐r

5.7.4 of the

Oxidizers

zing solid/liq

erally by yie

n peroxide,

a laboratory

s of oxidizing

5.7.7 of the

Organic Per

nic peroxide

considered a

ave been rep

ions (mixtur

e liable to ex

urn rapidly;

e sensitive t

eact danger

5.7.7 of the

The officia

al is a therm

decomposit

CHP details

ctive Mate

terial is a liq

able or toxic

, potassium)

reactive mat

CHP details

quid is a solid

lding oxygen

nitric acid, a

y. Sodium ni

g solids com

CHP details

roxides

is an organic

a derivative o

placed by org

res). Such su

xplosive dec

to impact or

rously with o

CHP details


mally unstabl

ion even wit

the storage

erials

uid or solid

gas, or othe

) and metal h

terials found

the storage

d/liquid whic

n, cause or c

and nitrate s

trate, Sodiu

mmonly foun

oxidizer sto

c liquid or so

of hydrogen

ganic radica

ubstances an

composition

friction; or

other substa

organic pero

89

ocument will only

e liquid or so

thout partici

requiremen

that reacts v

er hazardous

hydrides (e.g

d in laborato

requiremen

ch, while in i

contribute to

olutions are

m perchlora

d in a labora

rage require

olid which co

peroxide, w

ls. The term

nd mixtures m

;

nces

oxide storag

y be maintained on

olid liable to

ipation of ox

nts for reacti

violently wit

s conditions.

g., calcium h

ories.

nts for reacti

itself is not n

o the combu

e examples o

ate, and Pota

atory.

ements.

ontains the

where one or

m also include

may:

ge requireme

Appendix B: OSHA

nline.

o undergo a

xygen (air).

ive chemica

th water

. Alkali

hydride)

ive chemica

necessarily c

ustion of oth

of oxidizing l

assium perm

bivalent ‐0‐0

r both of the

es organic p

ents.

A Hazard Class Def

strongly

ls.

ls.

combustible

her material.

iquids comm

manganate a

0‐ structure

e hydrogen

eroxide

finitions

e,

monly

re

and

EB.1.8

An explo

of substa

gas at su

damage t

when the

designed

combina

reactions

(2,4,6‐tri

If a labor

before an

B.2 H

A chemic

study con

health ef

discussed

IrB.2.1

Irritants a

on living

organic a

powder o

reddenin

SB.2.2

A sensitiz

reaction

include d

and allyli

symptom

xplosives

sive substan

ances) which

ch a temper

to the surro

ey do not ev

d to produce

tion of these

s. An explosi

nitrophenol

ratory plans

ny work occ

Health H

cal is a healt

nducted in a

ffects may o

d below.

rritants

are defined

tissue by ch

and inorgani

or crystalline

ng or discom

ensitizers

zer (allergen

in normal ti

diazomethan

ic halides, an

ms associated

The officia

nce (or mixtu

h is in itself c

rature and p

undings. Pyr

volve gases. A

an effect by

e as the resu

ive compoun

).

to work with

urs.

Hazards

h hazard if t

accordance w

ccur in expo

as chemical

hemical actio

c compound

e form, are i

fort of the s

n) is a substa

ssue after re

ne, chromium

nd many phe

d with allerg


ure) is a solid

capable by c

ressure and

rotechnic su

A pyrotechn

y heat, light,

ult of non‐de

nd that is so

h explosive c

here is statis

with establis

osed employ

s that cause

on at the site

ds, including

rritants. Sym

skin and irrit

ance that cau

epeated exp

m, nickel, fo

enol derivati

gic reactions

90

ocument will only

d or liquid su

hemical reac

at such a sp

bstances are

nic substance

, sound, gas

etonative, se

metimes fou

compounds,

stically signi

shed scientif

ees. Each he

reversible i

e of contact.

g many chem

mptoms of e

ation to resp

uses exposed

osure to the

rmaldehyde

ives. Sensitiz

, or can incr

y be maintained on

ubstance (or

ction of prod

peed that ca

e included e

e (or mixture

or smoke or

elf‐sustaining

und in a labo

, contact RE

ficant evide

fic principles

ealth hazard

nflammator

. A wide vari

micals that ar

xposure can

piratory syst

d individuals

e substance.

e, isocyanate

zer exposure

rease an indi

Appendix B: OSHA

nline.

r mixture

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n cause

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r a

g, exotherm

oratory setti

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nce based o

s that acute

d is defined a

ry effects

iety of

re in a

n include

tems.

s to develop

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es, arylhydra

e can lead to

ividual’s exis

A Hazard Class Def

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on at least on

or chronic

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p an allergic

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o all of the

sting allergie

finitions

acid

ns

ne

s

ylic

es.

CB.2.3

Corrosive

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a

St

a

D

p

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HB.2.4

Substanc

H

as

N

ki

N

n

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w

S

PB.2.5

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be estab

establish

into thre

1. C

Corrosives

e substances

and can be e

trong acids s

cids

trong bases

mmonium h

Dehydrating a

hosphorus p

Oxidizing age

5.7.6 of the

Hazardous

ces with toxi

Hepatotoxins

s nitrosamin

Nephrotoxins

idneys, such

Neurotoxins,

ervous syste

ubstances th

which decrea

ubstances th

Particularly

ces that pose

us substance

lished to pre

hment of des

e primary ty

arcinogens

The officia

s cause dest

either acidic

such as sulfu

such as sod

hydroxide

agents such

pentoxide an

ents such as

CHP details

Substance

c effects on

s, which are

nes and carb

s, which are

h as certain h

which are s

em, such as

hat act on th

ase hemoglo

hat damage

y Hazardou

e such signif

es" (PHSs). T

event the ha

signated are

ypes:


ruction of liv

or caustic (b

uric, nitric, h

ium hydroxi

sulfuric acid

nd calcium o

hydrogen pe

corrosives s

es with Tox

specific org

substances t

bon tetrachlo

substances

halogenated

ubstances th

mercury, ac

he hematopo

bin function

lung tissue s

us Substan

ficant threat

The OSHA La

armful expos

as for their u

91

ocument will only

ving tissue b

basic). Major

ydrochloric

de, potassiu

d, sodium hy

oxide

eroxide, chlo

storage requ

xic Effects

ans include:

that produc

oride.

that cause d

hydrocarbo

hat produce

rylamide, an

oietic system

n and depriv

such as asbe

nces

s to human

boratory Sta

sure of resea

use. Particul

y be maintained on

by chemical c

r classes of c

and hydrofl

um hydroxid

ydroxide,

orine, and br

uirements.

on Specifi

:

e liver dama

damage to th

ons.

e toxic effect

nd carbon di

m (e.g., carbo

e the body t

estos and sili

health are c

andard requ

archers to PH

larly hazardo

Appendix B: OSHA

nline.

corrosion at

corrosive su

uoric

e, and

romine

ic Organs

age, such

he

ts on the

isulfide.

on monoxid

tissues of ox

ica.

classified as "

ires that spe

HSs, includin

ous substan

A Hazard Class Def

t the site of

bstances

e and cyanid

xygen.

"particularly

ecial provisio

ng the

ces are divid

finitions

des),

y

ons

ded

Appendix B: OSHA Hazard Class Definitions

92


2. Reproductive Toxins

3. Substances with a High Acute Toxicity

B.2.5.1 Carcinogens

Carcinogens are chemical or physical agents that cause cancer. Generally they are

chronically toxic substances; that is, they cause damage after repeated or long‐duration

exposure, and their effects may only become evident after a long latency period. Chronic

toxins are particularly insidious because they may have no immediately apparent harmful

effects. These materials are separated into two classes:

1. Select Carcinogens: Select carcinogens are materials which have met certain criteria

established by the National Toxicology Program or the International Agency for

Research on Cancer regarding the risk of cancer via certain exposure routes. It is

important to recognize that some substances involved in research laboratories are

new compounds and have not been subjected to testing for carcinogenicity.

2. Regulated Carcinogens: Regulated carcinogens are more hazardous and have

extensive additional requirements associated with them. The use of these agents

may require personal exposure sampling based on usage. When working with

Regulated Carcinogens, it is particularly important to review and effectively apply

engineering and administrative safety controls as the regulatory requirements for

laboratories that may exceed long term (8 hour) or short term (15 minutes)

threshold values for these chemicals are very extensive.

B.2.5.2 Reproductive Toxins

Reproductive toxins include any chemical that may affect the reproductive capabilities,

including chromosomal damage (mutations) and effects on fetuses (teratogens).

Reproductive toxins can affect the reproductive health of both men and women if proper

procedures and controls are not used. For women, exposure to reproductive toxins during

pregnancy can cause adverse effects on the fetus; these effects include embryolethality

(death of the fertilized egg, embryo or fetus), malformations (teratogenic effects), and

postnatal functional defects. For men, exposure can lead to sterility. Examples of

embryotoxins include thalidomide and certain antibiotics such as tetracycline. Women of

childbearing potential should note that embryotoxins have the greatest impact during the

first trimester of pregnancy. Because a woman often does not know that she is pregnant

during this period of high susceptibility, special caution is advised when working with all

chemicals, especially those rapidly absorbed through the skin (e.g., formamide).

B.2.5

Subst

dama

Acute

(LC50)

popu

chem

Chap

5.3 Subst

tances that h

age to target

e toxins are

), which is th

lation (e.g.,

mical that fal

A chemica

per kg of

test popu

A chemica

weight wh

hours to c

A chemica

of 200 pa

less of mi

continuou

are likely

foreseeab

ter 5.7.5 of t

The officia

ances with a

have a high

t organs as t

quantified b

he lethal dos

rats, rabbits

ls within any

al with a me

body weight

ulations.

al with an LD

hen adminis

certain test p

al with a me

rts per millio

st, fume, or

us inhalation

to be encou

ble manner.

the CHP det


a High Acute

degree of ac

the result of

by a substanc

se of a comp

s) over a spe

y of the follo

edian lethal d

t when adm

D50 of 200 m

stered by con

populations

edian lethal c

on (ppm) by

dust, when

n for one ho

untered by h

tails acutely

93

ocument will only

e Toxicity

cute toxicity

a single exp

ce’s lethal d

pound to 50%

ecified time p

owing OSHA‐

dose (LD50)

inistered ora

mg less per k

ntinuous con

.

concentratio

volume or l

administere

ur, provided

umans whe

toxic compo

y be maintained on

y are materia

posure or ex

ose‐50 (LD50

% of a labora

period. High

‐defined cat

of 50 mg or

ally to certa

kg of body

ntact for 24

on (LC50) in

less of gas o

ed to certain

d such conce

n the chemi

ounds storag

Appendix B: OSHA

nline.

als that may

posures of s

0) or lethal c

atory tested

h acute toxic

tegories:

r less

in

air

r vapor, or 2

n test popula

entration and

cal is used in

ge requirem

A Hazard Class Def

be fatal or c

short duratio

concentratio

d animal

city includes

2 mg per lite

ations by

d/or conditi

n any reason

ents.

finitions

cause

on.

on‐50

any

er or

on

nably

Appendix C: Peroxide Forming Chemicals

94


Appendix C:

Peroxide Forming Chemicals


95


Autoxidation in common laboratory solvents can lead to unstable and potentially explosive

peroxide formation. The reaction can be initiated by exposure to air, heat, light, or

contaminants. Most of these solvents are available with inhibitors to slow the peroxide

formation. Examples of inhibitors include BHT (2,6‐di‐tert‐butyl‐4‐methyl phenol) and

Hydroquinone. There are three categories of peroxide formers:

Group A chemicals are those which form explosive levels of peroxides after prolonged storage,

especially after exposure to air without concentration. Test these for peroxide formation before

using and discard 3 months after opening.

Table C.1 – Group A Chemicals

Butadiene Isopropyl ether

Chloroprene Tetrafluoroethylene

Divinylacetylene Vinylidine chloride

Group B chemicals form peroxides that are hazardous only on concentration by distillation or

evaporation. Test these before distillation and discard after 12 months.

Table C.2 – Group B Chemicals

Acetal Dicyclopentadiene Methyl isobutyl ketone

Acetaldehyde Diethyl ether 4‐Methyl‐2‐pentanol

Benzyl alcohol Diethylene glycol dimethyl ether 2‐Pentanol

2‐Butanol Dioxane 4‐Penten‐1‐ol

Cumene Ethylene glycol dimethyl ether 1‐Phenylethanol

Cyclohexanol 4‐Heptanol 2‐Phenylethanol

2‐cyclohexen‐1‐ol 2‐Hexanol 2‐Propanol

Cyclohexene Methylacetylene Tetrahydrofuran

Decahydronaphthalene 3‐Methyl‐1‐butanol Tetrahydronaphthalene

Diacetylene Methylcyclopentane Vinyl ether


96


Group C chemicals consist of monomers which form peroxides that can initiate explosive

polymerization. Inhibited monomers should be tested before use and discarded after 12

months. Uninhibited monomers should be discarded 24 hours after opening.

Table C.3 – Group C Chemicals

Acrylic acid Styrene

Acrylonitrile Tetrafluoroethylene

Butadiene Vinyl acetate

Chloroprene Vinyl acetylene

Chlorotrifluoroethylene Vinyl chloride

Methyl methacrylate Vinyl pyridine

General Guidelines

Solvents containing inhibitors should be used whenever possible.

All peroxide forming solvents should be tested prior to distillation.

Peroxide forming solvents should be purchased in limited quantities.

Peroxide forming solvents should be marked with the purchase date and the date

opened.

Peroxide forming solvents should be sealed tightly and stored away from light and heat.

Periodic testing should be done on opened containers and the results marked on the

containers.

Testing

Obtain test strips for the range of 0‐100 ppm peroxide.

Record the test results on the bottle.

If the test results are 100 ppm or greater, contact REM (765‐49‐40121) for proper

disposal.

Tab 1: Specific Standard Operating Procedures

Tab 1:

Specific Standard Operating Procedures

Tab 2: Lab‐Specific Protocols, Requirements, Rules

Tab 2:

Lab‐Specific Protocols, Requirements, Rules

Tab 3:

Lab‐Specific Hazard Assessments

CERTIFICATION OF HAZARD ASSESSMENT

HazAs

Supervisor (print): Patricia Bishop Assessment Date(s): September 12, 2014

Signature: Location(s) posted: BRWN 3154

Hazards

Task: hands-on work or being within reach(a) of potential hazards of described activity/items:

Minimum Requirements

Skin/eye damage, poisoning, inhalation of vapor or aerosol

Volume > 10 mL any unshielded(b) corrosive(c) liquids, organic liquids or liquid mixtures, or toxic(d) inorganic liquids/mixtures

Splash goggles, chemical resistant gloves(e), lab coat, skin cover to knees/elbows/throat, closed shoes with socks. Work in hood(f). Shower and eyewash must be available in work area.

Volume > 1 L Same, but cover to ankles/wrists/throat

Volume > 5 L Add face shield covering chin Skin/eye damage

Cryogenic liquids Splash goggles, skin cover to elbows/knees/throat, closed shoe easily removed, socks. Cryogloves for dispensing.

Volume > 1 L Skin cover to throat/wrists/ankles

Skin/eye damage, asphyxiation, body injury, frostbite,

Transport of liquid nitrogen in hallways and elevators

See cryogenic liquids; also all wheeled vessels or carts must restrain Dewar and have wheels large enough to safely traverse elevator door and scales gap.

Self pressurizing vessels weighing > 100 lb gross

Plus skin cover to wrists/throat/ankles, always position blow-off valve away from body

Dispensing from main bulk tank All above and hearing protection Frostbite, eye impact

Dry ice, very cold frozen solids. Safety glasses, insulated gloves, skin cover to elbows/knees/throat, closed shoe w/ socks

Eye damage, Erythema

UV radiation UV blocking goggles, skin cover on all potentially exposed areas

Potential face UV exposure UV face shield Skin/eye damage

Laser radiation Goggles appropriate to beam parameters, closed shoe, no jewelry/reflective items

Class 3b and 4 lasers Skin cover on all potentially exposed areas

Liquid with vol > 1 mL Same, but splash goggles, skin cover to throat/wrists/ankles

Skin/eye damage, poisoning, inhalation of airborne dust

Hazardous solids Safety glasses, goggles for large quantities, chemical resistant gloves, skin cover to elbows/knees/throat, closed shoes/socks

> 100 g any hazardous solid, or > 1 g "chemical requiring designated

area," (list at REM web site(g)) or High potential for airborne particles

Same, except skin cover to wrists/ankles, and only work in hood

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by PI/lab supe

rdinator/Lab M

his SOP:

SOP:

Hazardous C

/ Chemical P

perties:

osive

O4

quid

slightly yellow

4 °F)

rd Ope

lfuric aci

ot complete u

ol/procedure

gned and dat

insert into yo

ation

ervisor:

Manager:

hemical

Properties a

w

1

erating

id, conce

until: 1) lab sp

e is added to

ted by the PI

our Lab‐Specif

Chemistry

9/12/2014

10/1/2014

Pat Bishop

Pat Bishop

494‐7188

494‐7188

Pat Bishop

BRWN 315

☐ Hazard

and Uses

g Proce

entrated

pecific inform

the protocol

and relevant

fic Chemical H

y

4

4

p

p

p, 471‐2024

(Name a

54, 5106, 5185

(Buildin

dous Class

edure

d

mation is ente

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t lab personn

Hygiene Plan.

and Phone Numb

5

ng/Room Numb

Date: 3/19

ered into the

section and

el.

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ber)

er)

9/2014

box

Sulfuric acid

Flash Point

Relative Va

Uses:

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Section 4Highly corr

may cause

shortness o

burns, irrit

destructive

Section 5

Respirator

If Sulfuric a

activity is a

should be

As

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A

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As

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requireme

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4 – Potentiarosive chemica

irritation to th

of breath, and

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acid is being us

absolutely nece

used under an

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When Permissib

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egulations req

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to use/wear a

ww.purdue.edu

se proper glove

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uric acid) is a hemical propertiyte in lead‐acidncipal uses incsynthesis.

is harmful if in

tract with burn

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highly corrosivees, Sulfuric acid batteries, andclude mineral p

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to an atmosph

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booklets/RPP98

hnique to avoid

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d any skin cont

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ed through the

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age to the dige

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can be perform

ols have been

possibility tha

nant (in the abs

ested by REM.

tact. Nitrile glo

ct exposure. Ch

Date: 3/19

al name of thisations includinis also a centracturing, oil refi

skin. Inhalatio

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contact causes

stive tract. It is

quired. If this

med. Respirato

exhausted).

t PEL will be

sence of PEL)

This is a regula

oves are accept

heck the resou

9/2014

acid ng al ining,

on

s

s

ors

atory

table

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Sulfuric acid

NOTE: Con

with the sp

Refer to gl

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Eye Protec

ANSI appro

appropriat

Skin and B

Laboratory

Laboratory

should also

must be w

shoe and a

Hygiene M

Wash thor

water befo

Section 6

Use of Sulf

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velocity be

Section 7

If inhaled:

Move into

immediate

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shoes. Wa

In case of e

Check for a

consult a p

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ove selection c

w.ansellpro.co

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ction:

oved properly

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y coats must be

y coat sleeves m

o wear full leng

orn at all time

ankle should no

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roughly and im

ore removing c

6 – Engineer

furic acid shou

of vapor or mi

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7 – First Aid

the fresh air im

e medical atten

skin contact:

ely flush skin w

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eye contact:

and remove an

physician. Seek

r preferred glov

al being used.

chart from the

om/download/

glove.com/site/

com/

fitting safety g

on the specific

n:

e worn and be

must be of suff

gth pants, or e

s by all individ

ot be exposed.

mediately afte

clothes.

ring Controls

ld be conducte

ist. The chemic

25 feet per min

Procedures

mmediately. C

ntion.

with plenty of w

minated clothin

ny contact lens

k immediate m

ve manufactur

links below:

/Ansell_8thEdit

/default.aspx

glasses or chem

application.

appropriately

ficient length t

quivalent, and

uals that are o

.

er handling. Rin

s

ed in a properl

cal fume hood

nute.

onsult a physic

water for at lea

g before reuse

ses. Rinse thoro

edical attentio

3

rer to ensure th

tionChemicalR

mical splash gog

sized for the i

to prevent skin

d close‐toed sh

occupying the la

nse immediate

y functioning c

must be appro

cian. If not bre

ast 15 minutes

e. Thoroughly c

oughly with ple

on.

hat the gloves

ResistanceGuid

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ndividual and

n exposure wh

oes. Full lengt

aboratory area

ely contaminat

chemical fume

oved and certif

athing give art

while removin

clean shoes be

enty of water f

you plan on u

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ired. A face sh

buttoned to th

ile wearing glo

h pants and clo

a. The area of s

ed clothing an

e hood whenev

fied by REM an

tificial respirat

ng contaminate

efore reuse. Co

for at least 15

Date: 3/19

sing are compa

ield may also b

heir full length

oves. Personne

ose‐toed shoe

skin between t

d skin with ple

ver possible. Av

nd have a face

ion and seek

ed clothing and

onsult a physici

minutes and

9/2014

atible

be

el

s

the

enty of

void

d

ian.

Sulfuric acid

If swallow

Do NOT ind

person. Se

Section 8

Do no

Avoid

Alway

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Note:

Do no

Use in

Keep c

Conta

Store

Sulfur

oxidizi

nitrate

mater

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indica

legend

Transp

bottle

Section 9

Chemical S

Immediate

the neares

to personn

(dial 911 if

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Remove cl

dial 911.

Chemical S

Immediate

by forcibly

Section 1

Life Threat

Dial 911

d

ed:

duce vomiting

ek immediate

8 – Special H

t over purchas

contact with s

ys use inside a c

t allow water t

In case you ne

t use with met

n the smallest p

container uprig

iners which are

in original cont

ic acid must be

ing agents, red

es, perchlorate

rials, Zinc salts,

iners should be

te the name of

d is present in

port all corrosi

carrier.

9 – Spill and

Spill Dial 911

ely evacuate ar

st fire alarm sta

nel, contact RE

f spill occurs af

Spill on Body o

othing and rins

Splash into Eye

ely rinse eyes a

holding the ey

10 – Medica

tening Emerge

unless directe

medical attent

Handling and

se; only purcha

skin and eyes. A

chemical fume

to get into the

eed to dilute th

tal spatula or o

practical quant

ght & tightly cl

e opened must

tainer. Do not

e segregated fr

ducing agents,

es, permangan

and flammab

e labeled appr

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the lab) and fo

ves in seconda

Accident Pr

rea and ensure

ation to evacua

M at 49‐40121

fter hours and

or Clothes:

se body thorou

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and inner surfa

ye open. Seek

l Emergency

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d Storage Re

ase what can b

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container bec

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t be carefully r

store Sulfuric

rom incompati

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le liquids.

ropriately; the

(s) in the conta

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ate the buildin

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assistance is n

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medical attent

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urs, Weekends

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personnel. Nev

equirements

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ms.

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acid in metal c

ible materials.

nitrile, azides, c

ohydrin, Aniline

original manuf

ainer. Avoid us

nt, such as poly

ware of the spil

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al business hou

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gency shower f

ith water from

tion; dial 911.

s And Holidays

ver give anythi

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mist.

t reaction.

acid, always ad

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ated place.

ept upright to

containers.

Sulfuric Acid is

cyanides, chlor

e, carbides, fu

facturer’s labe

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yethylene or o

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urs (7 AM – 4 P

for at least 15

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tory.

dd the acid to w

prevent leaka

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rates, finely po

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abbreviations (

other non‐react

n imminent thr

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minutes. Seek

cy eyewash sta

Date: 3/19

o an unconscio

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(acceptable if a

tive acid/solve

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k medical atten

ation for 15 mi

9/2014

ous

ls,

organic

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ull

hreat

nce

ntion;

inutes

Sulfuric acid

Non‐Life T

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(http://ww

Section 1

Label Was

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streams. M

container.

Store Was

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times. To o

SDSs can b

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none

NOTE: Any

Section 1

Prior t

labora

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I have read

Pat Bisho

Hartmut

d

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ww.purdue.edu

11 – Waste D

te:

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Make sure the w

REM provides

te:

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a Chemical Wa

page for questi

12 – Safety D

copy of the SDS

obtain a copy o

be found online

Index (http://h

13 – Protoco

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14 – Docume

to conducting a

atory personne

tamination, an

rincipal Investi

rovided by the

rincipal Investi

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d and understa

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mergency:

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ot be disposed

waste containe

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Data Sheet (

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hazard.com/ms

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m this SOP req

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manufacturer

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quest Form to

ww.purdue.ed

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quires approva

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procedures.

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sher training w

nt of this SOP:

5

e the First Rep

drain. When po

y labeled; labe

e of charge, cal

designated are

arrange for dis

du/rem/hmm/w

ade available t

ical manufactu

‐Aldrich (http:/

al lab protoc

l from PI.

nature of all u

designated per

olved in workin

aboratory pers

her laboratory

within the last o

:

Sign

port of Injury.

ossible, do not

el should indica

l 49‐40121 to o

ea.

sposal by REM

wststo.htm)

to all personne

urer or REM at

//www.sigmaa

col may be a

users is require

rsonnel must p

ng with this sub

sonnel with a c

personnel hav

one year.

nature

mix acidic and

ate all of the co

obtain labels.

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t 49‐46371. Ma

aldrich.com/un

added here)

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provide trainin

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copy of this SO

ve attended ap

10/

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Date: 3/19

d basic waste

ontents of the

49‐40121 or vis

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any manufactu

nited‐states.htm

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OP and a copy o

ppropriate

Date

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sit the

at all

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Date: 3/19

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